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Journal : Bionature

The Effect of Synthetic Fertilizer Urea, TSP and ZA on Total Biomass and Chlorophyl Content of Dunaliella sp. Junda, Muhammad; Adhyani, Andi Nurul; Muis, Abd; Kurnia, Nani; Rachmawaty, Rachmawaty
Bionature Vol. 25 No. 1 (2024): April-Juni 2024
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v25i1.2229

Abstract

This research is an experimental research which aims to determine the effect of synthetic fertilizers urea, TSP and ZA on the amount of biomass and chlorophyll content of Dunaliella sp. on a laboratory scale. The research design used was a completely randomized design (CRD) consisting of 5 treatments with 3 replications each to obtain 15 treatment combinations. The research method consisted of several stages, namely preparation of tools and materials consisting of making culture media, making walne medium and making treatment medium, sterilizing tools and medium, preparing Dunaliella sp inoculum. on Walne medium with a static system, as well as optimizing the growth of Dunaliella sp.. The parameters observed in this study were phytoplankton biomass (dry weight), total chlorophyll content, salinity, pH, temperature, and light intensity. Based on the research results, the highest average biomass was found in the P3 treatment, namely the Walne medium treatment with the addition of 105 ppm urea, 80 ppm TSP, and 80 ppm ZA which produced an average of 5.23 g/L. Furthermore, the highest average results for measuring chlorophyll content were found in treatment P4 with walne medium treatment which added 115 ppm urea, 75 ppm TSP and 75 ppm ZA which produced an average of 1.81 µg/mL. So it can be concluded that the synthetic fertilizers urea, TSP and ZA have an effect on the amount of biomass and chlorophyll content on the growth of Dunaliella sp.
Optimization of Pectin Extraction from Pomelo Peels (Citrus maxima) using Response Surface Methodology Pagarra, Halifah; Hartati; Rachmawaty, Rachmawaty; Sahribulan, Sahribulan
Bionature Vol. 25 No. 1 (2024): April-Juni 2024
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v25i1.2340

Abstract

Pectin is an essential polysaccharide that is usually found in the skin of various fruits and vegetables. It acts as a gelling agent and stabilizer in many food and pharmaceutical industries. This study focuses on the potential of grapefruit peel as a source of pectin. Pectin is isolated from hot grapefruit peel powder (albedo). The aim of this study was to extract pectin from grapefruit peel (albedo), and central composite design (CCD) together with the numerical optimization method of response surface methodology (RSM) was used to optimize the processing parameters, including temperature (60, 80 and 100 ° C), pH (1.5, 2.0 and 2.5 ) and time (60, 90 and 120 min) The findings show that the studied parameters have a major impact on the pectin yield. The resulting second-order polynomial model thoroughly explains the relationship between input and output variables and how the data is varied. The highest pectin yield was 28.45% at optimal conditions achieved: temperature: 100° C; pH: 1.5; and extraction time: 120 minutes. Temperature, pH, and extraction time have their own large impact on pectin yield which has a significant effect.
Co-Authors A. Irma Suryani Abd Muis Abda Abda Abdul Muis Adhyani, Andi Nurul Aditya Wardhana Ahmad, Siti Nurfadhilah Mujaahidah Akriyono, Phintoko Akhbar Al Faruq, Abdullah Jundi Al Mubarak, Faqih Ramdhani Alimuddin Ali Alma, Genta Alyanra, Ananda Azzahra Amiruddin, Ilhamsyah Amraeni, Amraeni Andi Bida Purnamasari Andi Muflihunna Angka, Andi Werawe Arifah Novia Arifin, Arifah Novia Aris, Affandy Agusman Arsal, A.Faridah Asham Bin Jamaluddin Baharsyah, Vivi Salvia Baharuddin*, Riski Aprilianti Baharuddin, Riski Aprilianti Cahyadi, Eko Danang Yuangga, Kharisma Danendra, Ravi Darwiwing, Muh. Darwiwing Dina Novita, Dina Djabir, Muh Djafar, Djafar Dwiyana, Aldi Fathur Rahman Febrianto, Satriyadi Fiddah, Andi Izatul Firdaus Daud Gunawan, Matthew Marcellinno Haeruddin, Muhammad Ilham Wardana Halifah Pagarra Hamsinah Hamsinah, Hamsinah HARTATI Hartati Hartati Hartiwiningsih Hartiwiningsih Hartono Hartono Hasan, Nurhidayah Hasanah, Dillah Faradillah Hasanah, Dilllah Faradilla Havizh, Muhammad Hilda Karim Hiola, Siti Fatmah Hiola, St.Fatmah Hisni, Dayan Holiawati, Holiawati I Gusti Ayu Ketut Rachmi Handayani Idris, Irma Suryani Irawan, Panji Irnawati, Jeni Islami, Firda Jaja Suteja Jati, Waluyo Jumadi Jumadi K, Andi Sumarlin Kartono , Koerul Anwar Lasmi, Lasmi Lisdawati Lisdawati Lodang, Hamka M. Risal, M. Mamis, Yonartilia Mapata, Dg. Marjohan, Masno Mattunruang*, Andi Aris Melinda Pratiwi, Melinda Mokhamad Anwar, Mokhamad Mufa’ati, Nurma Dewi Muhammad Junda Muhiddin Palennari Nani Kurnia, Nani Nugroho, Arief Novianto Nur Arisah Nur Izzah Nurdiana Nurdiana Nurhayati B Nurhayati, Hesti Nurmin Arianto, Nurmin Nurviani, Novi Oslan Jumadi Pingki Indrianti Pratiwi AR, Dhita Rahma Rahma Rahmawati, Rahmawati Ramadan , Warisatun Retnaning Sampurnaningsih, Sri Retno Widowati Riandi, Arif Riesso, Andi Sawe Roos, Harjo Nakulo Muhammad Ramadhan Putra Wika Roslina Roslina, Roslina Rudy Kurniawan Rusilowati, Umi Sahribulan, Sahribulan Sahroni, Sahroni Saleh, Andi Rahmat Sampurnaningsih, Sri Retnaning Saparuddin Saparuddin Saputri, Winda Eka Sarah, Sitti Shafa Chairisa HSB, Anas Siti Nuraini Situmorang, Santonius St. Fatmah Hiola Sugiarto S Sugiyanto - Suharto Suharto Sumiati Side Suradi Suradi Susanto Susanto Sutiman Sutiman, Sutiman Syamsiah, Syamsiah Syamsu, Andi Mu'nisa Syatoto, Iman Syawaluddin, Ahmad Tabsir, Muhammad Kusnady Watye, Rina Yanti, Novi Misna Yusminah Hala Yusuf, Yusnaeni Zaerofi, Afif