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Potensi Ekstrak Etanol Daun Murbei (Morus alba L.) sebagai Antifungi terhadap Jamur Candida albicans Utami, Yuri Pratiwi; Rante, Herlina; Handayani, Tuti; Jariah, Ainun; Erianto, Fatricia
Journal of Experimental and Clinical Pharmacy (JECP) Vol 4, No 1 (2024): February 2024
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v4i1.969

Abstract

Senyawa kimia di daun murbei (Morus alba L.), termasuk flavonoid, tanin, steroid, dan saponin. Salah satu golongan senyawa yaitu flavonoid memiliki sifat antioksidan, antibakteri, dan sifat antimikroba yang dapat meningkatkan kontraksi luka. Penelitian dilakukan untuk mengetahui bagaimana potensi daun murbei sebagai antifungi terhadap jamur Candida albicans. Untuk menghasilkan ekstrak yang akan diskrining fitokimia, teknik maserasi menggunakan pelarut etanol 70% digunakan untuk ekstraksi sampel. Uji bebas etanol dan dilanjutkan dengan pengujian antifungi terhadap jamur Candida albicans. Hasil ekstraksi memperoleh persen rendemen ekstrak 3,89%. Pengujian skrining fitokimia mengandung flavonoid, saponin dan tannin. Pengujian ekstrak dengan konsentrasi 1,25%, 2,5%, 5% dan 10% memiliki aktivitas sebagai antifungi terhadap Candida albicans yaitu 9,7 mm ± 0,36, 10,83 mm ± 0,45, 15,8 mm ± 3,31, 26,06 mm ± 3,27 dan ketokonazole sebagai kontrol positif 33,4 mm ± 0,57. Disimpulkan bahwa ekstrak etanol daun murbei memiliki aktivitas sebagai antifungi terhadap Candida albicans. Ekstrak etanol daun murbei dapat menghambat pertumbuhan Candida albicans hingga konsentrasi 1,25%, sehingga dapat dimanfaatkan sebagai antijamur.Chemical compounds in mulberry leaves (Morus alba L.) include flavonoids, tannins, steroids, and saponins. One class of compounds, namely flavonoids, has antioxidant, antibacterial, and antimicrobial properties that can increase wound contraction. Research was conducted to determine the potential of mulberry leaves as an antifungal against the fungus Candida albicans. To produce extracts that will be screened for phytochemicals, a maceration technique using 70% ethanol solvent is used for sample extraction. Ethanol-free test and continued with antifungal testing against Candida albicans fungus. The extraction results obtained an extract yield percentage of 3.89%. Phytochemical screening tests contain flavonoids, saponins, and tannins. Extract testing with concentrations of 1.25%, 2.5%, 5%, and 10% had antifungal activity against Candida albicans, namely 9.7 mm ± 0.36, 10.83 mm ± 0.45, 15.8 mm ± 3.31, 26.06 mm ± 3.27, and ketoconazole as a positive control (33.4 mm ± 0.57). It was concluded that the ethanol extract of mulberry leaves has antifungal activity against Candida albicans. Mulberry leaf ethanol extract can inhibit the growth of Candida albicans up to a concentration of 1.25%, so it can be used as an antifungal.
Identifikasi dan Penetapan Kadar Flavonoid Ekstrak Etil Asetat Daun Mimba (Azadiractha indica A. Juss) Metode Spektrofotometri UV- Vis Utami, Yuri Pratiwi; Imrawati, Imrawati; Mustarin, Rahmah; Mus, Suwahyuni; Jariah, Ainun
Journal of Experimental and Clinical Pharmacy (JECP) Vol 4, No 2 (2024): August 2024
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v4i2.1049

Abstract

Flavonoid terdiri dari 15 molekul yang tersusun dalam susunan C6-C3-C6 yang merupakan senyawa polifenol. Senyawa ini berbentuk kerangka karbon dan terurai menjadi dua set: C6 dan C3. Daun mimba (Azadiractha indica A. Juss) mengandung flavonoid yang memiliki sifat antioksidan, antibakteri, dan antikanker. Penelitian ini bertujuan untuk mengidentifikasi kelompok senyawa dalam daun mimba (Azadirachta indica A. Juss) dan menentukan kadar flavonoidnya. Ekstraksi dilakukan menggunakan teknik maserasi dengan pelarut etil asetat untuk mendapatkan ekstrak yang kemudian diuji melalui skrining fitokimia. Pengukuran kadar flavonoid dilakukan dengan metode spektrofotometri UV-Vis. Dari hasil ekstraksi, diperoleh rendemen ekstrak sebesar 5,83%. Hasil skrining fitokimia menunjukkan adanya kandungan flavonoid dan alkaloid. Kadar flavonoid ditentukan menggunakan kuersetin sebagai standar pembanding, dengan persamaan garis y = 0,0739x + 0,0776. Kesimpulannya, ekstrak etil asetat mengandung flavonoid dengan kadar 1,183 ± 0,018%.Flavonoids consist of 15 molecules arranged in a C6-C3-C6 configuration, which are polyphenolic compounds. These compounds form a carbon framework and are divided into two sets: C6 and C3. Neem leaves (Azadirachta indica A. Juss) contain flavonoids that have antioxidant, antibacterial, and anticancer properties. This study aims to identify the group of compounds in neem leaves (Azadirachta indica A. Juss) and determine their flavonoid content. Extraction was carried out using the maceration technique with ethyl acetate as a solvent to obtain an extract that was then tested through phytochemical screening. Flavonoid content was measured using the UV-Vis spectrophotometric method. The extraction process yielded an extract with a percentage of 5.83%. Phytochemical screening results indicated the presence of flavonoids and alkaloids. The flavonoid content was determined using quercetin as a reference standard, with the equation y = 0.0739x + 0.0776. In conclusion, the ethyl acetate extract contains flavonoids with a concentration of 1.183 ± 0.018%.
Penyuluhan Kesehatan Tentang Gizi Pada Perempuan Sindroma Menopause Lusianawati, Hayu; Irmayanti, Irmayanti; Afni, Fauza; Pannyiwi, Rahmat; Andriani, Fitri; Utami, Yuri Pratiwi
Sahabat Sosial: Jurnal Pengabdian Masyarakat Vol. 1 No. 4 (2023): Sahabat Sosial: Jurnal Pengabdian Masyarakat (September)
Publisher : Asosiasi Guru dan Dosen Seluruh Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59585/sosisabdimas.v1i4.170

Abstract

Abstract Menopause is the final phase where a woman's menstrual bleeding stops completely. At the age of 50 years, women enter menopause, resulting in a decrease or loss of the estrogen hormone, which causes women to experience complaints or disorders that often interfere with their daily activities and can even reduce their quality of life. Several factors that influence mothers not being ready to accept changes during menopause include education, low knowledge, work, attitudes, religion, the role of midwives, social culture and information that is still lacking. One effort to increase mothers' readiness to face menopause is to changing lifestyle and eating patterns, namely by consuming nutrients that can reduce menopausal symptoms and prevent problems that arise after menopause. The aim of this activity is to increase mothers' knowledge about balanced nutrition during menopause. The method for implementing community service activities used is in the form of counseling. After carrying out this community service activity, conclusions that can be drawn include an increase in knowledge about balanced nutrition during the menopause. The hope is that menopausal mothers can adopt a healthy lifestyle by eating nutritious food and doing physical exercise, for example seniors' exercise. By adopting a healthy lifestyle, it is hoped that the quality of life of menopausal women will be better.
Sosialisasi Pelaksanaan Pengelolaan Obat Di Puskesmas Mamajang Aripa, Lusyana; Betan, Abu Bakar; Zaenal, Zaenal; Wahyuni, Sri; Utami, Yuri Pratiwi; Imrawati, Imrawati
Sahabat Sosial: Jurnal Pengabdian Masyarakat Vol. 1 No. 4 (2023): Sahabat Sosial: Jurnal Pengabdian Masyarakat (September)
Publisher : Asosiasi Guru dan Dosen Seluruh Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59585/sosisabdimas.v1i4.171

Abstract

Drug management is a pharmaceutical service activity that starts from planning, procurement, distribution, use and disposal of drugs. The aim is to ensure the continuity and availability of efficient, effective and rational medicines, increase the competency of pharmaceutical staff, create a management information system and implement service quality control and implementation relationships in drug management at the Mamajang Community Health Center, Mamajang District, Makassar City. Collection was carried out through in-depth interview techniques and direct observation. The results of drug planning at the Mamajang Community Health Center are in accordance with the drug management guidelines which are seen based on needs at the Community Health Center. Requests for drugs are in accordance with the procedures for procuring/requesting drugs from the Health Service via LPLPO to the District Pharmacy Warehouse (GFK). The distribution of medicines at the Mamajang Community Health Center is not in accordance with the drug management guidelines from GFK and is not in accordance with the needs at the Community Health Center, due to the lack of budget from the APBD which is channeled through the City Health Service. The conclusion is that the planning and use of drugs is in accordance with procedures, while the distribution of drugs is not in accordance with drug management guidelines because the APBD budget is not channeled through the Health Service. So it is hoped that when distributing medicines from the Makassar City Health Service or (GFK) they should pay attention to the procurement/request for medicines based on the LPLPO. The high population ratio in Pamenang, Tanjung, and Gangga Districts compared to existing health facilities and personnel is a very principal problem related to health education for the community, including information about health. One of them is about the correct procedures for obtaining, using, storing and managing medicines which includes how to use special medicines. The aim of this socialization is to educate human resources at the Mamajang Community Health Center. Socialization of this program is carried out through the delivery of material, discussions, questions and answers, demonstrations by pharmacists. The participants happened to be human resources at the Community Health Center who were very enthusiastic about this program because of the participants' activeness in asking and answering questions about drug management.
Pendidikan Kesehatan, Pemeriksaan dan Deteksi Dini Pencegahan Penyakit Tidak Menular melalui Kegiatan Olah Raga Fisik di Wilayah Kerja Puskesmas Tamalanrea Makassar Hilal, Achmad; Rachman, Arif; Hardianti, Hardianti; Sari, Lely Meriaya; Imrawati, Imrawati; Utami, Yuri Pratiwi
Sahabat Sosial: Jurnal Pengabdian Masyarakat Vol. 2 No. 4 (2024): Sahabat Sosial: Jurnal Pengabdian Masyarakat (September)
Publisher : Asosiasi Guru dan Dosen Seluruh Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59585/sosisabdimas.v2i4.417

Abstract

Non-communicable diseases are a group of diseases that cannot be transmitted, but non-communicable diseases are the main cause of death in 68% of the world. In fact, currently the incidence of non-communicable diseases has reached ±38 million sufferers and is predicted to continue to increase to reach 52 million people in 2030. This activity aims to increase people's knowledge and healthy living behavior regarding non-communicable diseases, empower health cadres and community health centers to carry out early detection of non-communicable diseases through anthropometric measurements, and reduce the prevalence and complications of non-communicable diseases in the community. The benefits of this activity are providing accurate and easy to understand information about non-communicable diseases through leaflets, improving people's health and quality of life, and supporting government programs in preventing and controlling non-communicable diseases. The methods used in this activity are health checks, nutritional counseling and community service demonstrations through training and counseling activities with Health Education, Examination and Early Detection, Prevention of Non-Communicable Diseases through Physical Sports Activities in the Tamalanrea Makassar Health Center Working Area. The results of the education were assessed by completing a pre-post education questionnaire and a significant increase in scores was obtained, meaning that the education carried out had increased the knowledge of women of childbearing age and the elderly regarding non-communicable diseases and their prevention.
Pendampingan dan Sosialisasi dalam Pemberian Edukasi dengan Leaflet (brosur) Penggunaan Obat Tetes Telinga Suminar, Saka; Utami, Yuri Pratiwi; Surya, Sara; Mustarin, Rahmah
Sahabat Sosial: Jurnal Pengabdian Masyarakat Vol. 2 No. 4 (2024): Sahabat Sosial: Jurnal Pengabdian Masyarakat (September)
Publisher : Asosiasi Guru dan Dosen Seluruh Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59585/sosisabdimas.v2i4.450

Abstract

Various drug-related problems can be caused by the community's lack of understanding about the use and proper handling of drugs. Providing education on how to use special drugs such as ear drops is very important because there are critical points in ear drops, especially in the method of instillation. Providing incomplete and unclear information on how to use drugs will result in less than optimal therapy so that the drug will not achieve the desired therapeutic effect. This community service activity aims to increase public knowledge about how to use ear drops properly. This service is carried out by providing information through leaflets and explaining directly to the community. Providing education on how to administer ear drops to the community received a good response because most people do not know how to use them properly. The presence of 36 participants and the participants who attended were very enthusiastic in listening to the explanation and actively asked questions related to the use of ear drops and their handling. This can support the realization of government programs in improving health services for the community. This counseling received significant results. Therefore, the PkM TEAM hopes that with this counseling, it can share information about the correct use and handling of ear drops with their family members.
Study of Narcotics Management in One of the Private Pharmacies in Makassar City Mustarin, Rahmah; Utami, Yuri Pratiwi; Massolo, Selpi
International Journal of Health Sciences Vol. 2 No. 2 (2024): IJHS : International Journal of Health Sciences
Publisher : Asosiasi Guru dan Dosen Seluruh Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59585/ijhs.v2i2.364

Abstract

Drug management is an important aspect of management, because its inefficiency will have a negative impact on health facilities both medically and economically. Drug management includes the stages of planning, procurement, receipt, storage, delivery, destruction, control, recording and reporting. The purpose of this research is to determine the suitability of the management of Narcotic drugs in one of the Makassar City Pharmacies with the regulations stipulated in the Minister of Health Regulation No.73 of 2016 concerning pharmaceutical service standards in Pharmacies and the Food and Drug Supervisory Agency No.4 of 2018 concerning management supervision drugs, medicinal substances, narcotics, psychotropics and pharmaceutical precursors in pharmaceutical service facilities. The type of research used was descriptive using interview and direct observation methods at one of the Makassar City pharmacies. From the results of interviews and observations, the management of narcotic drugs in pharmacies is in accordance with the regulations stipulated in the Minister of Health Regulation No. 73 of 2016 concerning standards of pharmaceutical services in pharmacies and the Food and Drug Supervisory Agency No. 4 of 2018 concerning supervision of the management of drugs and materials. drugs, narcotics, psychotropics and pharmaceutical precursors in pharmaceutical service facilities.
KLT-BIOAUTOGRAFI EKSTRAK DAUN PATIKALA (Etlingera elatior (Jack) R.M. Sm) MENGGUNAKAN VARIASI PELARUT Yuri Pratiwi Utami; Imrawati Imrawati; Hamdayani Lance Abidin; Tuti Handayani
JURNAL KATALISATOR Vol. 8 No. 2 (2023): Jurnal Katalisator Volume 8 No. 2, Oktober 2023
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v8i2.2459

Abstract

Tanaman patikala (Etlingera elatior (Jack) R.M. Sm.) digunakan masyarakat sebagai obat penyakit kulit dan antimikroba pada pangan. Telah banyak dipelajari senyawa antibakteri dari tanaman tetapi antibakteri dengan analisis KLT Bioautografi dari daun patikala belum banyak yang mempelajarinya.  Tujuan dari penelitian ini melakukan analisis untuk mengetahui potensi daun patikala dari berbagai jenis pelarut berdasarkan tingkat kepolaran yang berperan sebagai antibakteri. Metode ekstraksinya adalah maserasi dengan beberapa pelarut yaitu n-heksan, etil asetat, dan etanol. Kemudian dilakukan aktivitas antibakterinya menggunakan analisis KLT-Bioautografi. Analisis golongan senyawa antibakteri  dilakukan dengan KLT-Bioautografi kontak menggunakan eluen n-heksan (7) : etil asetat (3) untuk ekstrak etanol dan n-heksan (8) : etil asetat (2) untuk ekstrak etil asetat dan n-heksan terhadap bakteri Staphylococcus aureus dan bakteri Escherichia coli. Diperoleh 4 noda untuk ekstrak etanol dengan Rf 0,3, 0,8, 0,9 dan 0,94, pada ekstrak etil asetat diperoleh 4 noda dengan Rf 0,2, 0,6, 0,8 dan 0,9 sedangkan pada ekstrak n-heksan diperoleh 5 noda dengan Rf 0,18, 0,6, 0,7, 0,9 dan 0,96. Hasil KLT-Bioautografi menunjukkan golongan senyawa yang bersifat antibakteri, pada ekstrak etil asetat ada pada Rf 0,2 dan pada ekstrak n-heksan ada pada Rf 0,18 . Dapat disimpulkan bahwa noda yang di identifikasikan sebagai antibakteri adalah golongan senyawa flavonoid
Antioksidan Ekstrak Daun Patikala (Etlingera elatior (Jack) R.M. Sm) Penangkal Radikal Bebas ABTS Asal Enrekang Sulawesi Selatan Yuri Pratiwi Utami; Risfah Yulianty; Yulia Yusrini Djabir; Gemini Alam
JURNAL KATALISATOR Vol. 8 No. 2 (2023): Jurnal Katalisator Volume 8 No. 2, Oktober 2023
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v8i2.2572

Abstract

Daun patikala (Etlingeraelatior Jack. R.M. Smith) adalah salah satu tanaman yang dapat digunakan untuk menangkal radikal bebas. Metabolit sekunder tanaman termasuk flavonoid, alkaloid, tannin, dan fenol, yang dapat berfungsi sebagai antioksidan, telah banyak dipelajari antioksidan dari tanaman tetapi antioksidan dari daun patikala belum banyak yang mempelajari dan mengkajinya. Tujuan penelitian ini adalah untuk menentukan kekuatan antioksidan ekstrak daun patikala (Etlingeraelatior Jack R.M. Smith) dalam meredam radikal bebas dengan metode ABTS, asal Enrekang Sulawesi Selatan merupakan asal sampel tanaman yang dietahui bahwa faktor tumbuh suatu tanaman mempengaruhi kadar metabolite sekunder yang berpotensi sebagai antioksidan. Proses ekstraksi dilakukan melalui maserasi menggunakan pelarut, yaitu etanol 70%. Untuk menilai aktivitas antioksidan, digunakan metode peredaman ABTS (2,2'-azino-bis-{3-etilbenzotiazolin-6- asamsulfonat}), diukur pada panjang gelombang 745 nm, lebih rendah dari panjang gelombang vitamin C (739 nm). Hasil yang diperoleh yaitu nilai IC50rata-rata sebesar 37,98 ppm untuk ekstrak etanol daun patikala. Aktivitas antioksidan ekstrak etanol daun patikala sangat kuat karena nilai IC50 kurang dari 50 ppm dan vitamin C 0,15 ppm.Kesimpulan pada penelitian ini yakni bahwa ekstrak etanol daun patikala berpotensi menangkal radikal bebas ABTS sehingga penelitian ini dapat berkonstribusi sebagai sumber informasi pemanfaatan daun patikala sebagai antioksidan.
Penetapan Sifat Fisikokimia Ekstrak Etanol Daun Mimba (Azadiractha indica A. Juss) : Penelitian Eksperimen Berskala Laboratorium Pratiwi Utami, Yuri; Imrawati, Imrawati; Jariah, Ainun; Mustarin, Rahmah; Bone, Mahfuzun; Bachri, Nurjannah
Health Information : Jurnal Penelitian Vol 16 No 3 (2024): September-Desember
Publisher : Poltekkes Kemenkes Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36990/hijp.v16i3.1298

Abstract

Daun mimba (Azadirachta indica A. Juss) banyak digunakan sebagai obat tradisional yaitu untuk mengobati malaria, kencing manis, diare, penurun panas, dan antitukak lambung. Aktivitas suatu ekstrak dapat dipengaruhi oleh senyawa aktif yang terkandung di dalamnya yang bergantung pada sifat fisikokimia dari ekstrak. Penelitian ini bertujuan untuk menentukan sifat fisikokimia ekstrak etanol daun mimba (A. indica A. Juss.). Penelitian bersifat experimental berskala laboratorium yang dimulai dengan sampel diproses hingga menjadi simplisia; kemudian, simplisia diekstraksi melalui metode maserasi dengan pelarut etanol 70%. Penetapan sifat fisikokimia meliputi beberapa parameter yaitu rendemen, organoleptik, kadar senyawa terlarut, penetapan kadar senyawa terlarut, skrining fitokimia, dan penetapan kadar flavonoid total. Hasil penelitian menunjukkan bahwa ekstrak etanol menghasilkan sifat fisikokimia yaitu rendemen ekstrak 19,438 %, organoleptik ekstrak (bentuk (kental); warna (hijau kehitaman); bau (khas); dan rasa (pahit)), penetapan kadar senyawa terlarut (senyawa terlarut air (62.5%); senyawa terlarut pada etanol (71.3%)), skrining fitokimia mengandung (alkaloid, flavonoid, saponin, steroid dan tannin), dan kadar flavonoid total ekstrak yaitu 1.829%.  Dapat disimpulkan bahwa ekstrak etanol daun mimba (A. indica A. Juss.) dapat ditetapkan sifatnya secara fisikokimia dengan beberapa parameter yang telah diuji untuk mengetahui karakteristiknya sehingga disarankan ekstrak ini dapat dikembangkan dalam bentuk sediaan obat bahan alam.
Co-Authors Abd Rasyid Abd. Halim Umar Abdul Halim Umar Abidin, Hamdayani Lance Abubakar Betan Afni, Fauza Agus Firmansyah Ainun Jariah Ainun Jariah Ainun Jariah Ainun Jariah Anto Apriyanto Aprilia Matelda Dwi Kristiyanti Arif Rachman Aripa, Lusyana Asril Asril Burhan Asril Burhan Astri, Andi Astri Sri Nurul Fardila Astuti Amin Benedikta Winne Angela Besse Yuliana Bone, Mahfuzun Burhanuddin Taebe Erianto, Fatricia Fadel Ahmad Haris Fadhilla Maryam Fadillah Maryam Fadillah Maryam Fajriansyah Fajriansyah Fhahri Mubarak Firdaus Fitri Andriani, Fitri Fitriyanti Jumaetri Sami Gemini Alam Hamdayani L. A Hamdayani Lance Abidin Hapiwaty, Saldi Hardianti Hardianti, Hardianti HARDIANTO - Heldawati Heldawati Herlina Rante Hilal, Achmad Humang, Rezki Ihsan Imrawati Imrawati Imrawati Imrawati Imrawati Imrawati Imrawati Imrawati Imrawati, Imrawati Indah Kadullah Irmayanti Irmayanti, Irmayanti Khairuddin Khairuddin L A, Hamdayani Lusianawati, Hayu Mahfuzun Bone Mangosa, Grace Pretty Marwati Marwati Mar’atun Jannah, Imrawati, Muzakkir Baitz, Massolo, Selpi Muh Azwar AR Mus, Suwahyuni Nasution, Ahmad Faisal Nur Aida Kubangun * Nurhayani Nurhedah, Nurhedah Nurhidaya Nurhidaya Nurjannah Bachri Nurjannah Bachri, Nurjannah Nurul Ainun Agustin Nurzadrina Wahyuddin Pannyiwi, Rahmat Prima Astuti Handayani Rahmad Aksa Rahmah Mustarin Rahman, Nur Fausia Rahmat, Rezqiqah Aulia Reny Syahruni Risfah Yulianty Rohana Rohana Rosadika Fatimah Rosdiana Rosdiana S, St. Mujahida Safidni, Elvira Saldi Hapiwaty Sari, Lely Meriaya Sarwati, Dewi Sima, Yenny Sri Wahyuni Suhaera Suminar, Saka Surya, Sara Suwahyuni Mus Suwahyuni Mus Tuti Handayani Tuti Handayani Zainal Wahyu Hendrarti Wahyuddin Wahyuddin Wahyuddin Yulia Yusrini Djabir Zaenal Zaenal, Zaenal Zahira Amody Zainal, Tuti Handayani Zulfahmidah Zulfahmidah