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All Journal Jurnal Sain Veteriner Jurnal Veteriner Microbiology Indonesia Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease Masyarakat, Kebudayaan dan Politik Jurnal Medik Veteriner Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary Journal of Pharmaceutical and Sciences. Journal of Fisheries & Marine
Aksono HP., Eduardus Bimo
Division Of Veterinary Basic Medicine, Faculty Of Veterinary Medicine, Universitas Airlangga, Surabaya-Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Journal : Jurnal Sain Veteriner

 1 
Respon Imun Mencit terhadap Protein 24 dan 71 kDa Toxocara vitulorum dalam Membentuk Antibodi dan Protektifitasnya terhadap Infeksi Buatan Candra Dwi Atma; Kusnoto Kusnoto; Eduardus Bimo Aksono HP.
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5854.416 KB) | DOI: 10.22146/jsv.34684

Abstract

This study aimed to get 24 and 71 kDa protein of T. vitulorum that have a high antigenicity and imunogenicity on ELISA and to get the protein which able to protect mice against artificial infection of L2 T. vitulorum. This study using mice Balb/c aged 6 to 8 weeks. Proteins isolated were 24 and 71 kDa. Proteins 24, 71 kDa and intestinal homogenates immunized in mice with the addition of adjuvant (1: 1) for 3 times with period of 2 weeks. Two weeks after the last booster, serum drawn from mice tested by Indirect ELISA to determine the value of optical density (OD). The next stage, mice were infected L2 with a dose of 10-17 larvae / g of body weight. The results showed the average OD value by ANOVA Factorial antigen P24 was not significantly different with antigen P71 T. vitulorum. Antigen 24 kDa and 71 kDa with different immunization, both were showed P0 significantly different with  P1, P2 and P3. Based on percentage of  L2 in the somatic tissue of mice, P0 were showed 79.1% of total number of L2 early infection, whereas the treatment of P1 were showed 0.04%, P2 and P3 showed as much as 0.02% and 0.04%. 24 and 71 kDa protein of T. vitulorum that have a high antigenicity and imunogenicity.
Co-Authors Adelina Grace Aditya Irawan Ahmad Thoriqul Firdaus Al arif, Mohammad Anam Al-Arief, Mohamad Anam Aldise Mareta Nastri, Aldise Mareta Amal, Mohammad Noor Azmai Ana Adelina Farahdiba Anwar Ma'ruf Anwar Ma'ruf Aprinda Ratna Lovela Aquaresta, Febriana Arianto, Intan Diah Safitri Aryaloka, Suhita Aryati Aryati Ayuti, Siti Rani Bambang Sektiari Lukiswanto Billa, Lutfiah Annisa Boedi Setiawan BUDI UTOMO Candra Dwi Atma Choirul Oktavian Setiyadin Nayiron Chusniati, Sri Dyah Ajeng Suhita Eka Pramyrtha Hestianah Endang Suprihati Erlita Rusnaningtias Ernawati, Rahaju Fajar Ramadhan Subiyantoro Fedik Abdul Rantam Firnanda, Ferian Gandul Atik Yuliani HAK HOTTA Hapsari, Nafisah Nurul Hasib, Abdullah Heni Puspitasari HERNOMO ONTOSENO KUSUMOBROTO Hestiana, Eka Pramyrtha Hidajati , Nove Hidajati, Nove Hidayatik, Nanik Ira Sari Yudaniayanti Ira Sari Yudaniyanti Ismudiono Iwan Sahrial Hamid Juniastuti Juniastuti Kadek Rachmawati Kadek Rachmawati Khairullah, Aswin Rafif Khanand, Nadeem Kuncorojati, Suryo Kurniawan, Muhammad Ridho Hafid Kusnoto Kusnoto Lastoaji, Firman Lilis Mundri Jannah, Lilis Mundri Lovela, Aprinda Ratna Lucia Tri Suwanti, Lucia Tri M. Gandul Atik Yuliani MARIA INGE LUSIDA Maria Inge Lusida Meles, Dewa Ketut Mirni Lamid Mochamad Lazuardi Moses, Ikechukwu Benjamin Mufasirin Muhammad Qushai Y. Matondang, Muhammad Qushai Y. Muhammad Rivai Nanik Hidayatik Nasronudin Nasronudin Niken Meyliana Sari Nove Hidajati Nufus, Faizah Zakiyyatun Nur Syamsiatul Fajar, Nur Syamsiatul Nurfitri, Yulia Nurhusien Yimer Nurrahmad, Nanda Rino Nusdianto Triakoso Perdana, Rizka Fathoni Pinayungan, Probo Probo Warih Tatag Poedji Hastutiek Pudji Srianto Puput, Sesa Rahmahani, Jola Rahmawati, Kadek Rochmah Kurnijasanti Rozi Salleh, Annas Sari, Aulia Puspita Sari, Putri Desi Wulan Sasmono, R. Tedjo Setiawan Koesdarto Shifa Fauziyah Sin War Naw SOETJIPTO . Sri Herawati Sri Pantja Madyawati Sri Subekti Srimaryanto, Leonardo Reza Sudar Itafarida Suherni Susilowati Sukmanadi, Mohammad Sunarso, Agus Suroso, Bakti Suwarno TAKAKO UTSUMI Tatik Hernawati Teguh Hari Sucipto, Teguh Hari Tita Damayanti Lestari Tri Wahyu Suprayogi Tyasningsih, Wiwiek Usma Nur Dian Rosyidah Wahyuni, Retno Sri Widiyatno, Thomas Valentinus Widya, Alicia Margaretta Wiwik Misaco Yuniarti Yohan, Benediktus YOSHIHIKO YANO YOSHITAKE HAYASHI Yuliani, Margareth Gandul Atik Yunus , Muchammad Yunus, Muchammad