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Aksono HP., Eduardus Bimo
Division Of Veterinary Basic Medicine, Faculty Of Veterinary Medicine, Universitas Airlangga, Surabaya-Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Journal : Journal of Fisheries

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Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico Approaches Rozi; Tyasningsih, Wiwiek; Rahmahani, Jola; Aksono, Eduardus Bimo; Yunus , Muchammad; Al-Arif, Mohammad Anam; Kuncorojati, Suryo; Kusdarwati, Rahayu; Sari, Putri Desi Wulan; Amal, Mohammad Noor Azmai; Salleh, Annas; Khanand, Nadeem; Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.62035

Abstract

Graphical Abstract Highlight Research The study aims to develop a multi-epitope vaccine (MEV) against A. hydrophila by targeting the aerolysin toxin, a key virulence factor responsible for infections in fish and humans. Computational methods identified and optimized B-cell and T-cell epitopes, focusing on their ability to trigger immune responses without causing toxicity or allergenicity. In silico simulations demonstrated that the MEV has a strong binding affinity to immune receptors like TLR-4, MHC-I, and MHC-II, indicating its potential to induce robust cellular and humoral immunity. Structural analysis of the MEV showed a stable 3D conformation, with most residues in favorable regions, ensuring stability during immune activation. The MEV could enhance disease control in aquaculture and reduce human infection risks, offering a promising solution to address antibiotic resistance and the absence of effective vaccines. Abstract Aeromonas hydrophila, gram-negative, is a major pathogen responsible for various diseases in mammals, reptiles, amphibia, and vertebrates, including fish and humans. Targeting the specific toxin aerolysin in A. hydrophila is crucial to address antibiotic resistance and the lack of adequate and protective vaccines against this intracellular pathogen. This study aimed to identify a multi-epitope vaccination (MEV) candidate targeting A. hydrophila aerolysin toxin to combat the disease effectively. Standard biochemical characterization methods and sequencing of the 16S rRNA, rpoB, and aerA genes identified the isolate AHSA1 as A. hydrophila. Subsequently, we identified B and T cell epitopes on the aerolysin protein and separately predicted MHC-I and MHC-II epitopes. The epitopes are then evaluated for toxicity, antigenicity, allergenicity, and solubility. The vaccine design integrated multi-epitope-based constructs, utilizing specialized linkers (GPGPG) and EAAAK linkers to connect epitope peptides with adjuvants in the cholera toxin B component, thereby enhancing immunogenicity. Ramachandran plots showed that 85.25% of the residues were located in the most favorable regions, which was followed by the generously allowed zone (1.30%), the additional allowed regions (10.80%), and the forbidden regions (2.65%), thus confirming the feasibility of the modeled vaccine design. Based on docking simulations, MEV had the highest binding and interaction energies with TLR-4, TLR-9, MHC-I, and MHC-II (-1081.4, -723.2, 866.2, -9043.3 kcal/mol). Based on computational modelling, we expect the Aerolysin MEV candidate design to activate diverse immune mechanisms, stimulate robust responses against A. hydrophila, and maintain safety. The significant solubility, absence of toxicity or allergic response, and minimal side effects in animal testing all contribute to the potential clinical utility of this vaccine candidate.  
Co-Authors Adelina Grace Aditya Irawan Ahmad Thoriqul Firdaus Al arif, Mohammad Anam Al-Arief, Mohamad Anam Aldise Mareta Nastri, Aldise Mareta Amal, Mohammad Noor Azmai Ana Adelina Farahdiba Anwar Ma'ruf Anwar Ma'ruf Aprinda Ratna Lovela Aquaresta, Febriana Arianto, Intan Diah Safitri Aryaloka, Suhita Aryati Aryati Ayuti, Siti Rani Bambang Sektiari Lukiswanto Billa, Lutfiah Annisa Boedi Setiawan BUDI UTOMO Candra Dwi Atma Choirul Oktavian Setiyadin Nayiron Chusniati, Sri Dyah Ajeng Suhita Eka Pramyrtha Hestianah Endang Suprihati Erlita Rusnaningtias Ernawati, Rahaju Fajar Ramadhan Subiyantoro Fedik Abdul Rantam Firnanda, Ferian Gandul Atik Yuliani HAK HOTTA Hapsari, Nafisah Nurul Hasib, Abdullah Heni Puspitasari HERNOMO ONTOSENO KUSUMOBROTO Hestiana, Eka Pramyrtha Hidajati , Nove Hidajati, Nove Hidayatik, Nanik Ira Sari Yudaniayanti Ira Sari Yudaniyanti Ismudiono Iwan Sahrial Hamid Juniastuti Juniastuti Kadek Rachmawati Kadek Rachmawati Khairullah, Aswin Rafif Khanand, Nadeem Kuncorojati, Suryo Kurniawan, Muhammad Ridho Hafid Kusnoto Kusnoto Lastoaji, Firman Lilis Mundri Jannah, Lilis Mundri Lovela, Aprinda Ratna Lucia Tri Suwanti, Lucia Tri M. Gandul Atik Yuliani Maria Inge Lusida MARIA INGE LUSIDA Meles, Dewa Ketut Mirni Lamid Mochamad Lazuardi Moses, Ikechukwu Benjamin Mufasirin Muhammad Qushai Y. Matondang, Muhammad Qushai Y. Muhammad Rivai Nanik Hidayatik Nasronudin Nasronudin Niken Meyliana Sari Nove Hidajati Nufus, Faizah Zakiyyatun Nur Syamsiatul Fajar, Nur Syamsiatul Nurfitri, Yulia Nurhusien Yimer Nurrahmad, Nanda Rino Nusdianto Triakoso Perdana, Rizka Fathoni Pinayungan, Probo Probo Warih Tatag Poedji Hastutiek Pudji Srianto Puput, Sesa Rahmahani, Jola Rahmawati, Kadek Rochmah Kurnijasanti Rozi Salleh, Annas Sari, Aulia Puspita Sari, Putri Desi Wulan Sasmono, R. Tedjo Setiawan Koesdarto Shifa Fauziyah Sin War Naw SOETJIPTO . Sri Herawati Sri Pantja Madyawati Sri Subekti Srimaryanto, Leonardo Reza Sudar Itafarida Suherni Susilowati Sukmanadi, Mohammad Sunarso, Agus Suroso, Bakti Suwarno TAKAKO UTSUMI Tatik Hernawati Teguh Hari Sucipto, Teguh Hari Tita Damayanti Lestari Tri Wahyu Suprayogi Tyasningsih, Wiwiek Usma Nur Dian Rosyidah Wahyuni, Retno Sri Widiyatno, Thomas Valentinus Widya, Alicia Margaretta Wiwik Misaco Yuniarti Yohan, Benediktus YOSHIHIKO YANO YOSHITAKE HAYASHI Yuliani, Margareth Gandul Atik Yunus , Muchammad Yunus, Muchammad