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All Journal Buletin Peternakan Majalah Ilmiah Peternakan Jurnal Veteriner Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Journal of the Indonesian Tropical Animal Agriculture Jurnal Biologi Tropis Agrisocionomics: Jurnal Sosial Ekonomi Pertanian Jurnal Pengabdian Magister Pendidikan IPA JITPI : Jurnal Ilmu Dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Science and Technology) Jurnal Aplikasi Dan Inovasi Ipteks SOLIDITAS Jurnal Abdi Insani Journal of Classroom Action Research Bioscientist : Jurnal Ilmiah Biologi AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Prosiding Konferensi Nasional PKM-CSR Jurnal Pijar MIPA CELEBES Agricultural Jurnal Pepadu Jurnal Pendidikan dan Pengabdian Masyarakat Journal of Islamic economics Perspectives (JIEP)
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Journal : Bioscientist : Jurnal Ilmiah Biologi

 1 
Perbandingan Metode Isolasi Phosvitin dari Kuning Telur Ayam Buras untuk Karakterisasi Aktivitas Bioaktif Andayani, I Gusti Ayu Sri; Iswaini, Dedy; Depamede, Sulaiman N; Kisworo, Djoko; Bulkaini, Bulkaini; Sriasih, Made
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14304

Abstract

This study aims to compare phosvitin isolation procedures from chicken egg yolk based on purity level, time efficiency, and functional activity. The procedures compared include previously published methods and modified methods developed in this study. Phosvitin was isolated from 10 samples of native chicken eggs collected from farms in the Mataram area using a stratified random sampling approach. The isolation process involved several sequential steps: sedimentation, centrifugation, dialysis, and lyophilization. A comparative analysis of the two procedures was conducted based on protein profile characteristics using SDS-PAGE and Fourier Transform Infrared (FTIR) spectroscopy to identify phosphate group interactions and metal ion binding. SDS-PAGE results showed that both procedures yielded α-phosvitin (35 kDa) and β-phosvitin (45 kDa) protein bands. FTIR spectra confirmed that both phosvitin samples contained high levels of phosphate groups, indicating strong phosphorylation and good protein stability. From a chemical and biological standpoint, the modified procedure showed no qualitative differences compared to the previously established method. However, the modified protocol was approximately 30 minutes faster and did not require a pH adjustment step, making it a simpler and more time-efficient alternative for isolating phosvitin from egg yolk.
Co-Authors A. Ashari, A. A. Rai S. Asih Abrori, Muh. Muazdzamzam Lil Agil, Alwi Akhyar Sutaryono Akhyar Sutaryono Aldian Maulana Alfian G. M, Aji Alya Maolani Ami Rahmawati Aminuyati Amir Rohman Andayani, I Gusti Ayu Sri Ardana P Ariana, Tirta Ashari Astawa, Astawa Audina Putri Azhary Noersidiq B. R. D Wulandani B.R.D Wulandari B.R.D. Wulandari Baiq Rani Dewi Wulandani Baiq Rani Dewi Wulandani Baiq Rani Dewi Wulandari Blegoh Julianto, Totok Bq Rani Bq. Rani, DW BRD BRD. Wulandani Buana, I Gusti Ngurah Agung Budi Indarsih Budi Indarsih Budi Rahayu Tanama Putri Caska - Chairussyuhur Arman D. A. WARMADEWI D. Kisworo D.W. Sari Dahlanuddin Depamede, Sulaiman N Dewi Wulandani, Baiq Rani DIAN MAULANA Djoko Kiswolro Djoko Kisworo Djoko Kisworo Djoko Kisworo Djoko Kisworo Djoko Kisworo Djoko Kisworo Djoko Kisworo Djoko Kisworo Dwi Anggara Ristami Dwyardi E. Suryanto Edi Suryanto Eliza Apriana Elsya Mayori Enny Yuliani Enny Yuliani Eny Yuliani Fahrullah Fahrullah Fajariswana Fiarani, Mesil Fitri Handayani Gde Bidura Ginting, Trydha Yanti HARYANTO Haryanto Haryanto Haryanto Haryanto Haryanto Haryanto Hasma Hasma Hermasyah I . W. Karda, I . W. I Gusti Nyoman Gde Bidura I K Sumadi I Ketut Sumadi I Ketut Wiryawan I N. T. ARIANA I Nym. Sadia I Nyoman Sumerta Miwada I Wayan Karda I Wayan Karda Ichsan Ichsan Imron Hadi Iswaini, Dedy Jurnal Pepadu K. Sumadi Kartika Kartika Kertanegara Ketut Gde, Wiryawan Ketut, Sumadi Lalu Ahmad Zainuri Lalu Naif Zaharani Liana Dewi Lina Permatasari M Ashari M. Alfatih Made Sriasih, Made Malta, Lilik Marpuah Maskur Maskur Maskur Maskur Maskur Maskur Maskur Maskur Mastur Mastur - Mastur Mastur Mastur Mastur Maulana Mohammad Hasil Tamzil Muhamad Aprizal Akbar Muhamad Ashari Muhammad Ashari Muhammad Dohi Muhammad Haikal Fikri Muhammad Yasin Muhzi Muhzi Multazam, Syamsul Mutia S N. Haiza N. W. Siti Ni Wayan Siti Noviani Adhiningsih Novizar Nazir O. B. I. P. A, Yanesti Parwati Patullah, Holi Permatasari, Lina Pertiwi, Indah Cahya Rabiatul Adawiyah Rahmawati, Lilik Rani Wulandani Rini Mastuti Rini Mastuti Rosyidi, A. Samiadi (Samiadi) Satifayanti, Nusiferra Septian, I Gede Nano Silvia Slamet Pratama Soeparno Soeparno Soeparno Soeparno Sri . Handayani Sukarne Sukarne Sukarne, Sukarne Sukirno SUKIRNO Sukirno Sukirno Sulaiman Ngongu Depamede Sumadi, I. K Sumirtha, Miwada supristiwendi supristiwendi Sutama Sutaryono, Yusuf Sutaryono, Yusuf Akhyar Syamsuhaidi Syamsuhaidi, Syamsuhaidi Tarmizi Tarmizi Twen O. Dami Dato Ulkiyah, Kalisom Utami, Rosdiana Vebera Maslami W. Yulianto Wahid Yiulianto Wahid Yuliant Wahid Yulianto Wahid Yulianto Wahid Yulianto Wahyu Akbar Wilya Wrasiati, Wrasiati Wulandani, Baiq Rani Dewi Yulianto, W Yulianto, Wahid Yusuf Sutaryono Zuana