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Rimbawan ,
Department Of Community Nutrition, Faculty Of Human Ecology, Bogor Agricultural University, Bogor 16680, Indonesia
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EKSTRAKSI KOMPONEN AKTIF SEBAGAI ANTIKANKER PADA SEL LESTARI KEONG MATAH MERAH (Cerithidea obtusa) Sri Purwaningsih; Rimbawan ,; Bambang P. Priosoeryanto
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol. 15 No. 2 (2008): Desember 2008
Publisher : Institut Pertanian Bogor

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Abstract

ABSTRAKKomponen bioaktif dari bahan alami telah banyak diteliti untuk digunakan sebagai obat antikanker. Penelitian mengenai komponen bioaktif dari keong matah merah (Cerithidea obtusa) sebagai antikanker belum pernah dilaporkan. Penelitian ini bertujuan: (1) Menentukan cara ekstraksi keong matah merah dengan beberapa pelarut (air, air + methanol, aseton, etil asetat, dan methanol), dan (2) Uji aktivitas zat aktif dari keong matah merah dalam melawan sel lestari kanker (K562, A 549, dan HeLa/cervix cancer). Dari penelitian dapat ditentukan bahwa waktu ekstraksi terbaik yang dipilih untuk penelitian adalah 72 jam dan perbandingan antara bahan baku dengan pelarut adalah 1:6 (w/v). Hasil penelitian menunjukkan bahwa nilai terbaik adalah ekstrak keong matah merah dengan pelarut aseton yang mempunyai daya hambat terbesar pada sel kanker (90.62% untuk HeLa/cervix cancer, 79.84% kanker paru/A 549, dan 76.71% untuk leukemia/K562) pada konsentrasi 25 ppm.Kata kunci: Antikanker, Cerithidea obtuse, ekstraksi, sel tumor lestari.ABSTRACTBioactive components derive from natural substances have been widely studied for the use as an anticancer drug. A study on a bioactive component from matah merah mollusks (Cerithidea obtusa) as an anticancer has not yet been reported. This research was intended to find the best method of extraction for the bioactive components of this animal. The research were divided into of two stages: (1) an extraction of matah merah mollusks by applying various solvents (water, water and methanol, acetone, ethyl acetate, and methanol), and (2) anti-proliferation ability of matah merah mollusks against tumor cell line (HeLa/cervix cancer, A 549, and K562). The results showed that the best extraction time was is 72 hours with the ratio of main material and solvents being 1:6 (w/v). The result also found that acetone of 25 ppm was the best solution resulted the strongest inhibiting power against cancer cells (90.62% against HeLa/cervix cancer, 79.84% against lung cancer/A 549, and 76.71% against leukemia/ K562.Key words: Anti-cancer, Cerithidea obtuse, extraction, tumor cell line.
Food and Zinc Intake, and Determinant of Zinc Status among Pregnant Women in Leuwiliang and Cibungbulang Sub-Districts, District of Bogor Aslis Wirda Hayati; . Hardinsyah; . Rimbawan
Forum Pasca Sarjana Vol. 25 No. 3 (2002): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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The objectives of this study were to analyze zinc status, nutrient dietary quality, zinc content, zinc intake, determinant of zinc status and implications for preventing zinc deficiency among pregnant women living in Leuwiliang and Cibungbulang sub-districts, district of Bogor. The research used base line data of 252 pregnant women from previous study entitled "Effect of Multi-nutrients Fortificated Food Supplement for Pregnant Women and Child Growth and Development in Leuwiliang and Cibungbulang". Additional data collected were local food potency, zinc content of 17 foods, and formal and nonformal local leaders opinion on future institution programs related to nutrition and health.Logistic regression was applied for determinants analysis.
Cooking Method, Food Intake and Iodine Status of Pregnant Women in Saparua, Central Maluku Intje Picauly; . Hardinsyah; Ali Khomsan; . Rimbawan
Media Gizi dan Keluarga Vol. 24 No. 1 (2000): Jurnal Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

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The objective of this study was to analyze food intake and its cooking method in relation to iodine status of pregnant women in an endemic goitre area. For this purposes 57 pregnant women of the second trimester were selected randomly in Saparua Sub-district, an endemic goitre area of Central Maluku. Iodine status measuerd based on urinary excretion of iodine (UEI). Iodine content of urine, water, salt and common food consumed were analyzed in analytical laboratory of the Center for Research and Development of Nutrition, Bogor. The results show that stir frying, boiling, frying, boiling and frying, and grill are common cooking methods used in the study area. Lost of iodine varies depend on cooking methods and type of foods cooked. Lost of iodine content from the first two cooking method was range from 20 to 50%; and applying the last three cooking method resulted in iodine lost from 57 to74%. The iodine lost from sea fish was higher than from the other foods. The iodine food sources commonly consumed are cassava, sea fish and ¡¥gnetum¡¦ (melinjo) leaves with the iodine contents 42,0-94,6 ƒÝg/100g. The mean intake of cassava, sea fish and gnetum leaves was 559,0; 133,3 and 42,0 g/cap/day respectively. The contribution of cassava, sea fish and vegetables on iodine intake of pregnant women was 52,6; 25,4 and 18,8% respectively. All pregnant women consumed salt with the mean intake 19,2 g/cap/day and mean iodine content o,41 ppm. The mean UEI of pregnant women was 64,9 ƒÝg/l with median 47 ƒÝg/l. Only 17,5% of pregnant women has UEI at normal category. The result of regression analysis showed about 50% variation of UEI was explained by iodine, calcium and iron intakes (r=0,68).
Identification of In Vitro Bioavailability of Zinc in Different Types of Eggs and Methods of Processing . Rimbawan; Cesilia Meti Dwiriani; Ennita Fahriza
Media Gizi dan Keluarga Vol. 24 No. 1 (2000): Jurnal Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

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This research was intended to asssess zinc content and zinc bioavailability from six kinds of egg processing (boiled, poached, salted, fried egg with shallow frying (&Atilde;&cent;&acirc;&sbquo;&not;&Aring;&ldquo;dadar&Atilde;&cent;&acirc;&sbquo;&not;&Acirc; and &Atilde;&cent;&acirc;&sbquo;&not;&Aring;&ldquo;ceplok&Atilde;&cent;&acirc;&sbquo;&not;&Acirc;), also &Atilde;&cent;&acirc;&sbquo;&not;&Aring;&ldquo;pindang&Atilde;&cent;&acirc;&sbquo;&not;&Acirc; on three types of egg (local hens egg, layer egg and ducks egg). An in vitro dialysis procedure was employed to determine zinc bioavailablity. The experimental design used was complete random design with factorial experimental and further statistical analysis by Duncan Multiple Range. The results indicated that zinc bioavailabilities from three types of egg with six kinds of egg processing were ranged from 3.60 to 19.01 percents. Zinc content was ranged from 1.15 to 3.12 mg/100 g in wet basis or 2.59 to 12.22 mg/100 g in dry basis. Frying egg decreased zinc bioavailability and zinc content significantly (p<0.05). Boiling and poaching of egg increased zinc bioavailability and zinc content significantly (p<0.05). The data suggest that chickens egg (local hen&Atilde;&cent;&acirc;&sbquo;&not;&acirc;&bdquo;&cent;s and layer egg) processed by boiling and poaching increased zinc bioavailability.
Iodine and Selenium Consumption and Its Relationship with Iodine Status of School Children in Endemic and Non Endemic Coastal Areas . Rimbawan; Ali Khomsan; Dadang Sukandar; Sri Sumarmi; Sri Wahyu Angga Dewi
Media Gizi dan Keluarga Vol. 24 No. 2 (2000): Jurnal Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

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The specific objectives of this research were to study food consumption; iodine and selenium content of foodstuffs; intake of nutrients (energy, protein, vitamin A, iodine and selenium); and status of iodine among elementary school children living in coastal areas of Pasuruan District, East Java Province. The study was also purposed to observe the correlation between nutrients consumption and iodine status. Kalirejo Village was chosen purposively to represent a non endemic area of IDD in which the Total Goitre Rate (TGR) was 45%, while Tambak Lekok Village represented a non endemic area of IDD with no cases of goiter signs. Sixty elementary school children in each area were randomly selected as samples to whom 24 hours food consumption recall and early morning urine were collected. Urinary Excretion of Iodine (UEI) was analysed from each sample to measure the status of iodine. Statistical method called t-test was applied to judge any difference between the data of consumption, nutrient adequacies, UEI level (iodine status) in both areas. Correlation between variables was also examined statistically by multiple linier regression test. For all kind of food of animal origins, samples living in IDD-non endemic area consumed more in term of quantity than that in IDD-endemic area. The adequacy levels for energy, protein, vitamin A, iodine and selenium in IDD-endemic area were 89.61%, 234.24%, 269.92%, 23.33% and 78.79% respectively; while those in IDD-non endemic area were 107.59%, 208.68%, 151.99%, 103.07% and 239.18% respectively. No significant (p>0.05) difference was observed between data of nutrients consumption, energy adequacy level and protein adequacy level obtained from an IDD-endemic area and those obtained from non endemic area. In contrast, consumption and adequacy level of vitamin A, iodine and selenium of IDD-endemic area were significantly different from those observed in IDD-non endemic area. Generally, iodine and selenium content of foodstuffs obtained from IDD-endemic area were lower than those from IDD-non endemic area. UEI of samples living in IDD-endemic area were 108,50 u/l. These was significantly different from those living in with non endemic area, which were 192 u/l. Consumption of energy, protein and vitamin A showed no significant influence on iodine status. Iodine and selenium consumption were very significantly influenced by iodine status. Iodine status was very significantly influenced by iodine consumption.Keywords: iodine, selenium, chool children
The Effect of Cooking Methods of Several Varieties of Rice on Zinc Bioavailability Determined Using Experimental Rats Deni Elnovriza; . Rimbawan; Emma S Wirakusumah; Dadang Sukandar
Media Gizi dan Keluarga Vol. 25 No. 1 (2001): Jurnal Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

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The specific objectives of the research were 1) to analyze zinc content of rice, 2) to analyze effect of cooking on zinc content of rice and 3) to identify the effect of cooking methods of rice on bioavailability of zinc. Zinc bioavailability was indicated by serum alkaline phosphatase activity, zinc serum and growth of Wistar rats. Boiled, steamed and puffed of Membramos, Cisadanes and Brown rice were used in this research. The results of this study showed that cooking methods affect zinc in rice. Zinc content decreased after cooking in all of cooking methods. The lost of zinc was found highest in puffed rice and lowest in steamed rice. When rats fed those rice-based diets, zinc serum was for all of treatments not significantly different. Measurement of serum alkaline phosphatase activity of rats fed steamed and boiled rice was significantly higher than control, but not for rats fed puffed rice. All rats fed treatment diets have higher growth than those fed control. Zinc bioavailability was found lower in puffed rice than steamed and boiled. Steamed rice has zinc bioavailability better than that of boiled and puffed.
KETERBATASAN RESPON HEMOGLOBIN DAN SENG SERUM TERHADAP SUPLEMENTASI SENG (IN) DAN BESI (FE) PADA ANAK USIA 6-24 BULAN Hadi Riyadi; Sudjana Sibarani; Rimbawan Rimbawan; Muhilal Muhilal
Media Gizi dan Keluarga Vol. 28 No. 1 (2004): Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

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ABSTRACT. The objective of  this study  was to investigate the efficacy of  iron and zinc supplementation on anemic status and zinc status of children aged 6-24 months. The study was a double-blind, randomized, placebo-controlled trial in which 173 children aged 6-24 months were randomly assigned to receive 15 mg Fe+ 15 mg Zn daily 30 mg Fe+30 mg Zn weekly or placebo for 5 months. As expected, no significant difference in the baseline hemoglobin and serum zinc concentration and nutrient intake between the three supplementation groups. In the children aged 6-24 months, micronutrient deficiencies were prevalent. Almost 50% of the children were anemic, and 20% of them were zinc deficient. The anemic children had a 2.5-fold greater risk of  zinc deficient than did the non-anemic children. This could indicate the existence of concurrent nutritional deficiencies. At the end of the 5mo supplementation period, the daily and weekly Fe+Zn supplementation tend to increased hemoglobin concentrations, but there were no significant difference compared with the placebo. The daily and weekly Fe+Zn supplementation did not significantly affect serum zinc concentration compared with the placebo.   Keyword: suplementasi, anemia, besi dan seng.
PENGARUH STEROL LEMBAGA GANDUM (Triticum sp.) TERHADAP PROFIL LIPIDA DARAH TIKUS Sri Anna Marliyati; Hidayat Syarief; Deddy Muchtadi; Latifah Darusman; Rimbawan Rimbawan; Bambang Pontjo Priosoerjanto
Media Gizi dan Keluarga Vol. 28 No. 2 (2004): Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

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ABSTRACT. The role of fitosterol in preventing cholesterol absorption and reducing atherosclerosis risk has been reported. This research was conducted to evaluate the effect of wheat germ sterol on the concentration of total cholesterol, LDL, HDL, triglycerides, and lesion formation in mice. In this research work. Sprague Dawley mice at the age of 2 months  (productive age) were used A random block design was employed with the following treatment on each experimental unit: Po (basal diet, negative contra/), PI (basal diet with cholesterol added, positive control), P2 (basal diet with cholesterol and ground wheat germ added), P3 (basal diet with cholesterol and wheat germ oil added). P4 (basal diet with cholesterol and wheat germ sterol added). 'and P5 (basal diet with cholesterol and wheat germ sterol supplemented margarine). Each mouse was d.esigned to intake 0.0343 g ofsterol daily, except those of negative control (Po) and positive control (PI). In order to increase cholesterol concentration in blood serum ofmice, 20 mglkg-body weight per day ofpropyltiourasil (PTU) was added into each diet. A set oftests was carried out to observe the concentrations of total cholesterol. LDL, HDL, and tryglycerides in blood serum of test animals, and mouse aorta micrographs. Results showed that wheat germ sterol could prevent the increase ofcholesterol in the blood ofmice. Feeding with sterol (P4) or sterol-supplemented margarine (P5) prevented the increase of total cholesterol after I month of experimentation, however, only P4 sustained the same condition after 2 months ofexperimentation. Feeding with wheat germ sterol (P4) was also the only treatment that prevented the increase of LDL cholesterol after 2 month of experimentation, and it was not different significantly with negative control (Po). Results of experimentation also showed that the concentration of HDL in serum blood of mice was not affected by wheat germ sterol. Unsurprisingly, there was no lesion ofatherosclerosis observed in all treatments used in this research. This result confirmed that mouse is resistant to atherosclerosis. Keywords: wheat germ sterol, mouse, total cholesterol, LDL, HDL, triglycerides. atherosclerotic lesions
PENGARUH INTERVENSI MAKANAN KUDAPAN TERHADAP PENINGKATAN KADAR GLUKOSA DARAH DAN DAYA INGAT ANAK SEKOLAH DASAR Lilik Kustiyah; Hidayat Syarief; Hardinsyah Hardinsyah; Rimbawan Rimbawan; Sri Hartati Suradijono
Media Gizi dan Keluarga Vol. 30 No. 1 (2006): Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

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ABSTRACT. The objective of this study was to analyze the effect of snack intervention on blood glucose level and memory improvement ofelementary school's students, Subjects of this study were 184 students offour (4) elementary schools (grade 6, 5 and 4) at Bogor District, West Java. The study employed a quasi-experimental design and followed experimental procedures to control the subject's food intake and motoric activity during the study period At the day of intervention, both control and intervention's subjects were ordered not to have breakfast at home. Intervention's subjects were provided with snack (buras, at 10.00 AM) which contained 381.7 kcal energy and 5 g protein, but control's subjects were not. Two types ofpsychological test (word andfigure) were applied twice (at 09.00 and 11.00 AM). Then, at the same time, subject's blood was taken to determine blood glucose, haemoglobin, and hematocrite levels. Interviews with subjects and their mothers were carried out to collect socioeconomic data and dietary intake. Result of the study indicated that snack intervention increased significantly (p<O.OI) blood glucose level (20.8 mgld/) approximately 1 hour after snack given. Blood glucose level significantly (p<O.OI) affected the word's and figure's memory performance. The higher the blood glucose level the better the memory performance. Keywords: Snack intervention, blood glucose level, memoryperformance, Elementary School Students
KONSUMSI PANGAN DAN SENG, SERTA DETERMINAN STATUS SENG IBU HAMIL DI KECAMATAN LEUWILIANG DAN CIBUNGBULANG, KABUPATEN BOGOR Aslis Wirda Hayati; Hardinsyah Hardinsyah; Rimbawan Rimbawan
Media Gizi dan Keluarga Vol. 31 No. 2 (2007): Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

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ABSTRACT. The main objectives of this study were to analyze zinc status, nutrient dietary, quality zinc content, zinc intake, and determinant factor of zinc status. The research was based on previous study on "Effect of Multi-nutrients Fortified Food Supplementation for Pregnant Women and Child Growth and Development" and  some additional data of food  zinc content. Logistic regression was applied for determinants analysis. The results show that zinc serum level was 0.9±04mgll. The prevalence of zinc deficiency was 21.8%. Food intake ofpregnant women was very low (30.8% of suggested serving). Zinc content offood ranged from O.2mg (fried tempeh) to 22.9mg (local snail) per 100g oft he dried foods. Zinc intake was 6.3mglday, similar to 32.8% recommended dietary allowance (RDA). The subjects who have zinc intake lower than it's RDA (l9.3mglday) were 99.2%. The determinant ofzinc status was household income . Keywords: food consumption, zinc intake, zinc status, pregnant women,
Co-Authors Adi Teruna Efendi Adi Teruna Effendi Adi Teruna Effendi Adi Winarto Agustino Agustino Ahmad Sulaeman Ainia Herminiati Ainia Herminiati Ainia Herminiati Akifa Laila Rusyda Ali Khomsan Angga Hardiansyah Angga Rizqiawan Anna Vipta Resti Mauludyani Aprinia Dian Nurhayati Ascobat, Purwantyastuti Asep Rusyana Aslis Wirda Hayati Astrid Noviana Astuti Lamid Azizi, Elsa Carla Bambang P. Priosoeryanto Bambang Pontjo Priosoeryanto Betty Yosephin Simanjuntak Bibi Ahmad Chahyanto Budi Setiawan Budi Setiawan Budi Setiawan Budi Setiawan Budi Setiawan Cassandra Permata Nusa Cesilia M Dwiriani Clara Meliyanti Kusharto Dadan Rohdiana Dadang Sukandar Damayanti, Rinda Daus, Firdaus Firdaus Deddy Muchtadi Deddy Muchtadi Deni Elnovriza DEWI APRI ASTUTI Dewi Kusumawati Dewi Kusumawati Diah M. Utari Diah M. Utari Diffah Hanim Dira Warisanti Dodik Briawan Drajat Martianto Ekowati Handharyani Emma S Wirakusumah Ennita Fahriza Eva Susanti Evi Damayanthi Evy Damayanthi Faisal Anwar Fajriaty, Deannisa Febrina Sulistiawati Firdaus Firdaus Firdaus Firdaus Fitrah Ernawati Fitrah Ernawati Fivi Melva Diana, Fivi Melva Gifari, Nazhif Hadi Riyadi Hardinsyah Harianti, Rini Hidayat Syarief Hidayat Syarief I Gusti Bagus Wiksuana I.Wayan T.Wibawan, I.Wayan Ika Heri Kustanti Ikeu Ekayanti Ikeu Tanziha Ikue Ekayanti Ilmi Dewi Astuti Ilmi, Ibnu Malkan Bakhrul Indhira Shagti Iswahyudi Iswahyudi Katrin Roosita Khaerul Fadly Khairina, Dini Nur Kharisma Tamimi Kharisma Tamimi Laeli Nur Hasanah Latifah Darusman Latifah K Darusman Latifah K. Darusman Leily Amalia Leily Amalia Furqon Lidwina Lidwina Lilik Kustiyah Linar Zalinar Udin Lubis, Anwar MADE ASTAWAN MARIA BINTANG Mira Dewi Mohamad Rafi Muhamad Rizal Martua Damanik Muhammad Aries Muhilal ., Muhilal Muhilal Muhilal Muhilal Muhilal Muhilal Muhilal Muhilal Muhilal Mukhlidah Hanun Siregar Mulya Agustina Mury Kuswari Mury Kuswari Muti'ah Mustaqimatusy Syahadah Nasir, Syifa Qolbiyah Nasution, Zuraidah Naufal Muharam Nurdin Nawaf Tresnanda Nazhif Gifari Ni Ketut Sutiari Ni&#039;ma Hilyatin Novitasari, Putri Nur Indrawaty Lipoeto Nuri Andarwulan Pertiwi, Fenti Dewi Picauly, Intje Pipih Suptijah Purwantyastuti Ascobat Purwantyastuti Purwantyastuti Purwantyastuti Purwantyastuti Puspo Edi Giriwono Putri Puncak Anjani Rahman, Dewi Rahmayani Rakhmawati FKR Ratu Diah Koerniawati Reisi Nurdiani, Reisi Resita Nurbayani Restu Pertiwi Riana, Nidya Ulfah Rosyanne Kushargina Safitri, Debby Endayani Sari, Eny Kurnia Siti Madanijah Sri Anna Marliyati Sri Hartati R. Suradijono Sri Nur Amalia Sri Purwaningsih Sri Sumarmi Sri Wahyu Angga Dewi Subangkit, Mawar Sudjana Sibarani Suryana, Suryana Susi Desminarti Susi Nurohmi Sutrisna, Aang SYAHRIAL SYAHRIAL Syartiwidya Syartiwidya TATI NURHAYATI Trini Suryowati Tunggul Waloya V Prihananto Vetnizah Juniantito Wiwin Winarsih Yunianto, Andi Eka Yusra Egayanti Zakiudin Munasir Zakiudin Munasir Zuraidah Nasution