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Potensi selada air (Nasturtium officinale R. Br) sebagai antioksidan dan agen anti proliferasi terhadap sel MCF-7 secara in vitro Rahman, Dewi Rahmayani; Rimbawan, Rimbawan; Madanijah, Siti; Purwaningsih, Sri
Jurnal Gizi dan Pangan Vol. 12 No. 3 (2017)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.893 KB) | DOI: 10.25182/jgp.2017.12.3.217-224

Abstract

This study aimed to determine the potential of watercress extract as an antioxidant and antiproliferation against cancer cells in vitro. Cells used in this study were breast cancer culture cells (MCF-7) and vero cells. Watercress was extracted using water solvent and 70% ethanol. Phytochemical test was performed on both types of extract. Toxicity test was measured using BSLT method, assessment of antioxidant activity was measured using DPPH method and inhibitory proliferation in cancer cell was measured using MTT Assay method. Analysis of the data using ANOVA, if there is a significant difference between treatments will be followed up with Duncan test. The results of phytochemical tests showed that watercress extracts had bioactive content namely flavonoids, tannins, saponins, and steroids. The result of BSLT test showed that the strongest extract toxicity was 70% ethanol extract with LC50 value of 406.02 ppm and had the highest antioxidant activity with IC50 value of 102.26 ppm, while proliferation inhibitory test found in MCF-7 breast cancer cells had IC50 value of 1.696 μg / ml and not toxic to vero cells. Result showed that the ethanolic extract of Nasturtium Officinale gave a significant effect on MCF-7 breast cancer cells inhibition (p=0,015) at concentration 50, 400, 800 and 1000 ppm. The mechanism of proliferative inhibition was associated with phytochemical content present in watercress. 
POTENSI NUTRASETIKAL SEBAGAI TERAPI PENDAMPING KANKER PAYUDARA: TELAAH SISTEMATIS Putri, Sefanadia; Siregar, Mukhlidah Hanun; Suryana, Suryana; Rimbawan, Rimbawan
Tirtayasa Medical Journal Vol 4, No 2 (2025): May
Publisher : Universitas Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62870/tmj.v4i2.29475

Abstract

Globally, breast cancer stands as the most common cancer and the leading cause of death for women. Currently, breast cancer treatment strategies focus more on the evaluation of clinical and pathological parameters rather than gene expression criteria. With advances in medical science and nutrition, great attention is being paid to foods and natural products that can improve health. Bioactive nutrients that provide protective benefits against breast cancer include polyphenols, retinoids, and polyunsaturated fatty acids (PUFAs). These compounds possess antioxidant, anti-inflammatory, immunomodulatory, and antitumor properties. Specific substances such as resveratrol, epigallocatechin 3-gallate, retinoids, and omega-3 PUFAs influence the characteristics of breast cancer and are involved in various cancer processes, including cell growth inhibition, inflammation, and angiogenesis.
Pengaruh Substitusi Tempe, Penambahan Puree Wortel dan Puree Daun Kelor terhadap Kandungan Protein, Serat Pangan, Zat Besi, dan Bioassesibilitas Zat Besi Sosis Sapi: Effect of Tempeh Substitution, Addition of Carrot Puree and Moringa Leaf Puree on Protein, Fiber, Iron Content, and Iron Bioaccessibility of Beef Sausage Azizi, Elsa Carla; Rimbawan, Rimbawan; Marliyati, Sri Anna
Amerta Nutrition Vol. 9 No. 1 (2025): AMERTA NUTRITION (Bilingual Edition)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/amnt.v9i1.2025.14-19

Abstract

Background: Cases of iron deficiency anemia among adolescent girls in Indonesia remain high. To address this issue, there is a need to formulate an inexpensive sausage product with relatively high protein, iron, and fiber content. Objectives: This research was conducted to analyze the effect of tempeh substitution, carrot puree addition, and moringa leaf puree addition on the protein content, dietary fiber, iron, and Fe bioaccessibility in beef sausages. Methods: This study employed a true experimental design to develop sausage formulations and analyze their protein content, dietary fiber, iron content, and iron bioaccessibility. A 3-factor factorial design was used, with factors including tempeh substitution, carrot puree addition, and moringa leaf puree addition. Results: The protein content in the sausages was not influenced by any individual factor or interaction between factors, with values ranging from 6.27% to 6.91%. Fiber content was only affected by tempeh substitution, ranging from 11.89 to 13.84 mg/100 g. Iron bioaccessibility was influenced by both tempeh substitution and moringa leaf puree addition, with bioaccessibility values ranging from 84.3% to 87.1%. The highest bioaccessibility was observed with 40% tempeh substitution, 20% carrot puree addition, and 6% moringa leaf puree addition. Conclusions: Tempeh substitution significantly affected fiber content, iron content, and iron bioaccessibility. Carrot puree addition had no effect on any parameter, while moringa leaf puree addition influenced only iron bioaccessibility.
Status Gizi, Pola Konsumsi, Aktivitas Fisik, Riwayat Kesehatan dan Fenotip Wanita Muda Obese di Kota Bandung: Nutritional Status, Eating Patterns, Physical Activity, Health and Phenotype History of Obese Young Women in Bandung City Novitasari, Putri; Rimbawan, Rimbawan; Hardinsyah, Hardinsyah; Riyadi, Hadi
Amerta Nutrition Vol. 9 No. 1 (2025): AMERTA NUTRITION (Bilingual Edition)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/amnt.v9i1.2025.109-118

Abstract

Background: The incidence of obesity in Bandung City is rising. The prevalence of obesity among women surpasses that of men, with a notable rise observed in the young adult demographic. Objectives: This study assessed the nutritional status, dietary habits, physical activity, health history, and phenotype of young obese women in Bandung City. Methods: This mixed-method design involved 88 obese individuals (body fat percentage >35%) aged 18-25 years of Sundanese ethnicity. The research was carried out from August to November 2023. The obtained data encompassed general subject characteristics, anthropometric measurements, body composition, dietary intake and physical activity information, health history, and phenotypic data. Comprehensive interviews were performed concerning dietary habits, physical activity, phenotypic history, nutritional status, and health history. Results: The participants' average age and body weight were 20.3 years and 81.5 kilograms, respectively. The average total fat percentage (%BF) was 38.3%. Occupation (p-value=0.033), weight (p-value=0.001), body mass index (p-value=0.001), waist circumference (p-value=0.001), hip circumference (p-value=0.001), visceral fat (p-value=0.001), resting metabolism (p-value=0.001), and energy (p-value=0.009), fat (p-value=0.043), and carbohydrate (p-value=0.021) intake were associated with total body fat. Conversely, the subject's physical activity, personal and familial history of chronic diseases, development of obesity, and parental obesity history exhibited no correlation with the subject's total body fat (p-value≥0.05). Conclusions: The subject’s occupation, body weight, body mass index, waist and hip circumference, visceral fat, resting metabolism, energy, fat, and carbohydrate intake were correlated with the subject's total body fat.
POTENSI NUTRASETIKAL SEBAGAI TERAPI PENDAMPING KANKER PAYUDARA: TELAAH SISTEMATIS Putri, Sefanadia; Siregar, Mukhlidah Hanun; Suryana, Suryana; Rimbawan, Rimbawan
Tirtayasa Medical Journal Vol 4, No 2 (2025): May
Publisher : Universitas Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62870/tmj.v4i2.29475

Abstract

Globally, breast cancer stands as the most common cancer and the leading cause of death for women. Currently, breast cancer treatment strategies focus more on the evaluation of clinical and pathological parameters rather than gene expression criteria. With advances in medical science and nutrition, great attention is being paid to foods and natural products that can improve health. Bioactive nutrients that provide protective benefits against breast cancer include polyphenols, retinoids, and polyunsaturated fatty acids (PUFAs). These compounds possess antioxidant, anti-inflammatory, immunomodulatory, and antitumor properties. Specific substances such as resveratrol, epigallocatechin 3-gallate, retinoids, and omega-3 PUFAs influence the characteristics of breast cancer and are involved in various cancer processes, including cell growth inhibition, inflammation, and angiogenesis.
FTO rs9939609 Genotypes: Impact on Body Composition and Inflammatory Markers Related to Cardiovascular Risk Novitasari, Putri; Rimbawan, Rimbawan; Hardinsyah, Hardinsyah; Riyadi, Hadi
Mulawarman International Conference on Tropical Public Health Vol. 1 No. 1 (2025): The 3rd MICTOPH
Publisher : Faculty of Public Health Mulawarman University, Indonesia

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Abstract

Background : Obesity is a significant global health issue, contributing to the prevalence of chronic diseases. Genetic factors such as the Fat-Mass Obesity-Associated (FTO) gene, particularly the rs9939609 variant, have been linked to increased susceptibility to obesity. Objective : This study examines the relationship between FTO rs9939609 genotypes (TT and AA/TA) and body composition, as well as markers of inflammation, in obese individuals. Research Methods/ Implementation Methods : Using a cross-sectional design, 38 obese young women aged 18-25 years from Bandung City were included. Anthropometric measurements were taken, and blood samples were analyzed for interleukin-6 (IL-6), high-sensitivity C-reactive protein (hs-CRP), and adiponectin (ADP) levels. Results : The findings indicate that the AA/TA variant group showed lower mean values for weight, body mass index, waist and hip circumferences, visceral fat, fat percentage, resting metabolic rate, and subcutaneous fat than the TT group; however, these differences were not statistically significant. Additionally, the AA/TA group displayed slightly higher waist-to-hip ratios and skeletal muscle percentages, although these differences were also non-significant. These results contrast with previous studies, suggesting the influence of the FTO gene on obesity may vary based on population and other contributing factors. No significant differences in body composition or biochemical markers were observed between the FTO rs9939609 genotypes. Conclusion/Lesson Learned : The limited sample size and focus on a single demographic may have impacted the findings. Future research should involve a larger and more diverse sample, including individuals with various nutritional statuses, to enhance comparison and increase the reliability of the results.
Potensi Asam Amino pada Tempe untuk Memperbaiki Profil Lipid dan Diabetes Mellitus Utari, Diah M.; Rimbawan, Rimbawan; Riyadi, Hadi; Muhilal, Muhilal; Purwantyastuti, Purwantyastuti
Kesmas Vol. 5, No. 4
Publisher : UI Scholars Hub

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Abstract

Prevalensi penyakit degeneratif dari tahun ke tahun meningkat akibat perubahan gaya hidup, khususnya perubahan pola makan. Walaupun tempe sebagai makanan tradisional Indonesia yang banyak dikonsumsi masyarakat karena mudah diperoleh dan harga terjangkau, tidak banyak yang mengetahui manfaat tempe bagi kesehatan. Tempe lama dikenal sebagai sumber protein yang dikonsumsi oleh kalangan masyarakat berpenghasilan rendah. Kajian lebih mendalam mengenai kandungan gizi dan manfaat tempe dan bagi kesehatan, khusus sebagai pencegah penyakit degeneratif perlu dilakukan. Proses fermentasi kedelai menjadi tempe mengakibatkan perubahan zat gizi dan non gizi yang mengakibatkan manfaat tempe jauh lebih baik dibandingkan kedelai. Protein tempe lebih mudah di cerna tubuh, sedangkan asam amino arginin yang meningkat hampir dua kali lipat pada tempe, sangat tinggi manfaatnya bagi kesehatan terutama dalam memperbaiki profil lipid dan diabetes mellitus. Mempertimbangkan hal tersebut tempe dapat dipertimbangkan sebagai pangan fungsional yang bermanfaat bagi kesehatan. Degenerative diseases prevalence had been arining over years. One of the causes is the life style changes including eating pattern. Tempeh, an Indonesian soybean traditionally fermented food, was known and consumed by almost all Indonesian people. However, only a few know the health benefit of tempeh. Tempeh was also well known and cheap protein source food affordable for the poor. Hence, there is a need to explore the nutritious content of tempeh and health benefit of it deeper such as in preventing degenerative diseases. Fermentation process of soybean to become tempeh had improved nutrient and non-nutrient contents that make tempeh better than soybean. Tempeh protein is more digestible than soybean and the arginine content increases twice, that could improve lipid profile and diabetes mellitus. Tempeh could be considered as functional food having health benefit.
Peranan Ultraviolet B Sinar Matahari terhadap Status Vitamin D dan Tekanan Darah pada Wanita Usia Subur Yosephin, Betty; Khomsan, Ali; Briawan, Dodik; Rimbawan, Rimbawan
Kesmas Vol. 8, No. 6
Publisher : UI Scholars Hub

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Abstract

Sinar ultraviolet B adalah sumber utama vitamin D, tetapi wanita usia subur yang bekerja di dalam ruangan mempunyai vitamin D yang rendah meskipun Indonesia negara tropis. Tujuan penelitian ini untuk mengevaluasi peranan paparan sinar matahari pada wanita usia subur terhadap status vitamin D dan tekanan darah. Desain penelitian yang digunakan adalah eksperimen tanpa kelompok kontrol pada 21 wanita sehat. Penelitian ini membandingkan status vitamin D dan tekanan darah sebelum dan setelah mendapat paparan sinar matahari pada wajah dan lengan tiga kali seminggu selama 12 minggu. Analisis data menggunakan uji t-berpasangan. Paparan sinar matahari dapat meningkatkan vitamin D. Serum 25(OH)D meningkat 15,9% dari 15.7 ng/dL menjadi 18,2 ng/dL. Paparan sinar matahari menurunkan tekanan darah sistolik (nilai p = 0,004) dan diastolik (nilai p = 0,011). Ultraviolet B dari sinar matahari 30 menit tiga kali seminggu selama 12 minggu dapat memperbaiki status vitamin D dan tekanan darah. Ultraviolet B sunlight exposure is a primary source of vitamin D, but women of childbearing age who worked in room every day had low serum vitamin D despite Indonesia is a tropical country. The objective of this study was to evaluate the role of sun exposure in women of childbearing age on vitamin D status, and blood pressure. An intervention before-after study without group control was conducted on 21 healthy women. This study compared vitamin D status, and blood pressure before and after receiving ultraviolet B (UVB) from sun exposure on the face and both arms three times a week for 12 weeks. Anthropometric parameter and blood pressure were measured, were determined at baseline and after 12 weeks of sun exposure. The effect of sun exposure can improve vitamin D. Serum 25 (OH)D increase 15.9% from 15.7 ng/dL to 18.2 ng/dL. Sun exposure significantly reduced systolic blood pressure (p value = 0.004), and diastolic blood pressure (p value = 0.011). Ultraviolet B from sun exposure for 30 minutes, 3 times a week for 12 weeks improves the vitamin D status, and blood pressure.
STATUS GIZI DAN DEFISIENSI KALSIUM PADA ANAK BALITA DI INDONESIA: SAMPEL RISKESDAS 2018 Arifin, Aya Yuriestia; Rimbawan, Rimbawan; Riyadi, Hadi; Retiaty, Fifi; Nurjanah, Nunung; Julianti, Elisa Diana; Nurhidayati, Nuzuliyati
Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research) Vol. 47 No. 1 (2024): PGM VOL 47 NO 1 TAHUN 2024
Publisher : Persagi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36457/pgm.v47i1.781

Abstract

Calcium is believed to play a role in growth, especially in children under five, and contributes to malnutrition issues in Indonesia. However, national-scale data are very limited to delve deeper into this matter. The study aims to examine the calcium status of children in Indonesia and their nutritional status, including stunting and underweight. A cross-sectional study design using secondary data from the 2018 Basic Health Research (Riskesdas) and Study of Nutrition Status of Children Under Five and School-Aged Children 2021, including serum calcium concentration data and anthropometric measurements of body length and body weight. The study subjects were children aged 0-59 months, totaling 550 samples. The study results show the mean value of calcium concentration was 10.00±1.10 mg/dL. As many as 8.2 percent of children experienced deficiency with calcium concentration <9.0 mg/dL. The proportion of stunted and underweight children was 23.27 percent and 9.82 percent, respectively. The average calcium concentration in stunted and underweight children was not significantly different from the normal scores of HAZ and BAZ in children. Calcium status, along with factors such as age and sex, did not show a relationship with nutritional status indicators like height-for-age (HAZ) and weight-for-age (WAZ). Although the prevalence of calcium deficiency among toddlers in Indonesia is relatively low, there are still children with calcium concentrations significantly below the normal range. Further studies are needed to figure out the contribution of calcium to children's growth.
RESPONS SEL EPITEL USUS (CMT-93) TERHADAP NUTRASETIKAL GALOHGOR Roosita, Katrin; R, Rimbawan; Djuwita, Ita; Damanik, M. Rizal; Kusharto, Clara M.
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2825

Abstract

Penelitian ini bertujuan membandingkan pengaruh nutrasetikal galohgor dalam bentuk serbuk (GS) dan ekstrak (GE) terhadap proliferasi, morfologi, dan ekspresi gen Aldh1a2 pada sel epitel usus (CMT-93). Galohgor serbuk (GS) dibuat dengan menghancurkan semua bahan dandikeringkan menggunakan drum-dryer sedangkan GE dibuat dengan mengeringkan semua bahan dengan oven, digiling, dan dimaserasi dengan etanol selama 3x24 jam. Perlakuan didasarkan pada konsentrasi akhir -karoten yang berasal dari GS dan GE masing-masing sebesar 0,5; 1,5; dan 5,0 M dalam larutan medium Dulbecco's Modified Eagle's medium (DMEM) yang dilengkapi serum (10%). Analisis proliferasimenggunakan asai 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dan ekspresi gen dianalisis dengan reverse transcriptasepolymerase chain reaction (RT-PCR). Hasil penelitian meunjukkan bahwa GE pada konsentrasi tinggi (5,0 M) secara signifikan (P0,05) dapatmenekan proliferasi dan memengaruhi morfologi sel CMT-93. Beta-karoten dalam GE dan GS memengaruhi ekspresi gen Aldh1a2 pada sel epitel usus CMT-93
Co-Authors Adi Teruna Efendi Adi Teruna Effendi Adi Teruna Effendi Adi Winarto Agustino Agustino Ahmad Sulaeman Ainia Herminiati Ainia Herminiati Ainia Herminiati Akifa Laila Rusyda Ali Khomsan Ali Khomsan Angga Rizqiawan Anna Vipta Resti Mauludyani Aprinia Dian Nurhayati Ascobat, Purwantyastuti Asep Rusyana Aslis Wirda Hayati Astuti Lamid Aya Yuriestia Arifin, Aya Yuriestia Azizi, Elsa Carla Bambang P. Priosoeryanto Bambang Pontjo Priosoeryanto Betty Yosephin Simanjuntak Bibi Ahmad Chahyanto Bohari Bohari Budi Setiawan Budi Setiawan Budi Setiawan Budi Setiawan Budi Setiawan Cassandra Permata Nusa Cesilia M Dwiriani Cesilia Meti Dwiriani Clara M. Kusharto Clara Meliyanti Kusharto Dadan Rohdiana Dadang Sukandar Damayanti, Rinda Daus, Firdaus Firdaus Debby Endayani Safitri Deddy Muchtadi Deddy Muchtadi Deni Elnovriza DEWI APRI ASTUTI Dewi Kusumawati Dewi Kusumawati Diah M. Utari Diah M. Utari Diffah Hanim Dijaya, Rendy Dionysius Subali Dodik Briawan Drajat Martianto Egayanti, Yusra Ekowati Handharyani Ekowati Handharyani Elisa Diana Julianti Emma S Wirakusumah Ennita Fahriza Eny Palupi Evi Damayanthi Evy Damayanthi Faisal Anwar Febrina Sulistiawati Fifi Retiaty Firdaus Firdaus Firdaus Firdaus Fitrah Ernawati Fitrah Ernawati Fivi Melva Diana, Fivi Melva Gifari, Nazhif Giriwono , Puspo Edi Hadi Riyadi Haerati, Nur Hardiansyah, Angga Hardinsyah Harianti, Rini Harianti, Rini Hidayat Syarief Hidayat Syarief Himarwan, Aditya I Gusti Bagus Wiksuana I.Wayan T.Wibawan, I.Wayan Ika Heri Kustanti Ikeu Ekayanti Ikeu Tanziha Ikue Ekayanti Ilmi Dewi Astuti Ilmi, Ibnu Malkan Bakhrul Indhira Shagti Iswahyudi Iswahyudi ITA DJUWITA Katrin Roosita Khaerul Fadly Kharisma Tamimi Laeli Nur Hasanah Latifah Darusman Latifah K Darusman Latifah K. Darusman Leily Amalia Leily Amalia Furqon Lidwina Lidwina Lilik Kustiyah Linar Zalinar Udin Lubis, Anwar M. Muslim MADE ASTAWAN MARIA BINTANG Mauludiya, Putri Nur Mira Dewi Mohamad Rafi Muhamad Rizal Martua Damanik Muhammad Aries Muhilal ., Muhilal Muhilal Muhilal Muhilal Muhilal Muhilal Muhilal Muhilal Muhilal Mukhlidah Hanun Siregar Mulya Agustina Mury Kuswari Muti'ah Mustaqimatusy Syahadah Nasution, Zuraidah Naufal Muharam Nurdin Ni Ketut Sutiari Ni&#039;ma Hilyatin Noviana, Astrid Novitasari, Putri Nunung Nurjanah Nur Indrawaty Lipoeto Nurhidayati, Nuzuliyati Nuri Andarwulan Pertiwi, Fenti Dewi Picauly, Intje Pipih Suptijah Purwantyastuti Ascobat Purwantyastuti Purwantyastuti Purwantyastuti Purwantyastuti Putra, Felicia Kartawidjaja Putri Puncak Anjani Rahman, Dewi Rahmayani Rakhmawati FKR Ratu Diah Koerniawati Reisi Nurdiani, Reisi Resita Nurbayani Restu Pertiwi Rian Adi Pamungkas Riana, Nidya Ulfah Rini Kurniasih, Rini Rizal M Damanik, Rizal M Rosyanne Kushargina Safitri, Debby Endayani Seprianto Siti Madanijah Sri Anna Marliyati Sri Hartati R. Suradijono Sri Nur Amalia Sri Purwaningsih Sri Sumarmi Sri Wahyu Angga Dewi Subangkit, Mawar Sudjana Sibarani Suryana, Suryana Susi Desminarti Susi Nurohmi Susilawati, Esih SYAHRIAL SYAHRIAL Syartiwidya, Syartiwidya Tande, Ribka TATI NURHAYATI Tresnanda, Muhammad Nawaf Trini Suryowati, Trini Tunggul Waloya Umam, Hafizul V Prihananto Vetnizah Juniantito Vetnizah Juniantito Warisanti, Dira Wiwin Winarsih Yunianto, Andi Eka Zakiudin Munasir Zakiudin Munasir Zuraidah Nasution