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All Journal Microbiology Indonesia Indonesian Journal of Human Nutrition Jurnal Biosains Pascasarjana Indonesian Journal of Tropical and Infectious Disease Majalah Kulit, Karet, dan Plastik BERITA BIOLOGI Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Health Notions Journal of Applied Food Technology Indonesian Journal of Medicine Jurnal Respirologi Indonesia Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia JPPS (Jurnal Penelitian Pendidikan Sains) JPPIPA (Jurnal Penelitian Pendidikan IPA) Majalah Biomorfologi (Biomorphology Journal) Jurnal Psikiatri Surabaya (Surabaya Psychiatry Journal) MAHESA : Malahayati Health Student Journal Berkala Penelitian Hayati Jurnal Pendidikan Ilmu Pengetahuan Alam (JP-IPA) Jurnal Natural Jurnal Keperawatan Soedirman (The Soedirman Journal of Nursing) Folia Medica Indonesiana
SOETJIPTO .
Departemen Biokimia Kedokteran, Fakultas Kedokteran, Universitas Airlangga, Surabaya
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Energy Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Journal : Jurnal Natural

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HIV Genotype Analysis from HIV Infected Patients in East Java Area Yulia Sari Ismail; Soetjipto Soetjipto; Eddy Bagus Wasito; Nasronudin Nasronudin
Jurnal Natural Volume 12, Number 2, September 2012
Publisher : Universitas Syiah Kuala

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Abstract

Human Immunodeficiency Virus type 1 (HIV-1) has been known to cause Acquired Immune Deficiency Syndrome (AIDS) disease and has been alaso divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with host can affect the severity of the disease. This study was aimed to analyze HIV-1 genotypes circulating in HIV/AIDS patients from the East Java region descriptively. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia.  This study used blood plasma from patients who had been tested to be HIV positive who were seeking treatment or are reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant genotypes in East Java belonged to one group of Circulating Recombinant Form (CRF), namely CRF01_AE and CRF3x_01B, which has been also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) were in the same branch with CRF01_AE and CRF3x_01B, except one sample (HIV40) was in the same branch with subtype B.
Co-Authors Abdurachman Abdurachman Adi, Ginanjar Sasmito Aksono HP., Eduardus Bimo Amin, Indah Binti Mohd Amin, Mochammad Aminuyati Anang Endaryanto Anggraeni, Zuhrotul Eka Yulis Annis Catur Adi Annisa Rizky Maulidiana Arumisore, Netty M Asnita, Asnita Aulanni'am, Aulanni'am Ayesie Natasha Zulka Bakhtiar, Arief Bakhtiar, Arief Bayu Satria Wiratama Budi Yanti Choirunisa, Latifatu Chorisma Permata Putri Nurazizah Citriana, Mirna Yunik Dachlan Y P Damba Bestari Dienanta, Savira Eddy Bagus Wasito Eddy Wasito, Eddy Elvaretta, Clarissa Ely Rahmatika Nugrahani Ely Rahmatika Nugrahani, Ely Rahmatika Era Catur Prasetya Fedik Abdul Rantam Fitria Sarnita G. Panggabean Ginanjar Sasmito Adi HAK HOTTA HANSON, J. Harianto Notopuro Hendri Susilo Hendy Hendarto Hermanto, Fery Herniyati HERNOMO ONTOSENO KUSUMOBROTO Huda, Khusnul Humairah, Ira Ida Bagus Narmada Indri Safitri Indri Safitri, Indri Juminten Saimin Juniastuti Juniastuti Katharina Merry Apriliani Khaerunnisa, Siti Kirana, Tjandra Lukitasari, Lina Lukitasari, Lina Luqman Hakim Andira M. THAMRIN HIDAYAT Machin, Abdulloh Maghfiroh, Faridatul Marcos, Imella MARIA INGE LUSIDA Maria Inge Lusida MOCHAMAD AMIN Mufiannoor, Eddy Muhammad Amin Muhammad Faizi, Muhammad Muhammad Hamdan Murbani, Indri Dwi MUSLIMIN IBRAHIM Nailufa, Yuyun Nasronudin Nasronudin Ni Made Mertaniasih Nur Rochmah, Nur Palupi, Dian Eka Roro Poernomo Boedi Setiawan Prabowo, Gwenny Ichsan Priyo Budi Purwono Putri, Hanif Rafika Qurnianingsih, Ema Raharjo Raharjo Rahmawati, Siti Irma Rantam F A Retno Handajani Ridhwan Biosia Wiratama Rini Devijanti Ridwan Ruslan, S. Eriaty N. Saifullah Saifullah Samsriyaningsih Handayani Setiobudi, Karen Delicia Setyawati, Dewi Sidarningsih Sidarningsih, Sidarningsih Sifak Indana Siti Khaerunnisa Sufiyah, Sufiyah Sulistyorini, Anik Susi Wahyuning Asih Susi Wahyuning Asih, Susi Wahyuning Suwandito Suwandito Suwandito, Suwandito Syafa'ah, Irmi Syafa’ah, Irmi TAKAKO UTSUMI Titut Harnanik Tjandrakirana Tjandrakirana Tuti Kusumaningsih Ulfa Kholili Ummah, Fithriyah Cholifatul Ummi Maimunah W Widia Wahyudi, Lindung Wasis Wasis Waskito, Langgeng Agung WULIJARNI, N. WULUARNI, N. Wungu, Citrawati Dyah Kencono Wungu, Citrawati Dyah Kencono Yasan, Yanna Debby Restifanny YOSHIHIKO YANO YOSHITAKE HAYASHI Yudhi Kurniawan Yudi Her Oktaviono, Yudi Her Yulia Ismail Yulia Sari Ismail Zuhrotul Eka Yulis Anggraeni Zulka, Ayesie Natasha