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Isolation of Phosphate-Solubilizing Fungi from Forest Soils of the Harau Valley Roza Yolanda; Dezi Handayani; Azwir Anhar; Irdawati Irdawati
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The decline in the quality of agricultural products due to nutrient deficiencies such as phosphate is a major problem for agriculture in Indonesia. Technological advances have produced a way to overcome phosphate availability, namely by using phosphate-solubilizing fungi. Organic acids produced by phosphate-solubilizing fungi help reduce phosphate binding in the soil. This study aims to determine the activity of phosphate-solubilizing fungi isolated from Harau Valley Forest soil. Soil sampling was done by the purposive sampling method. The sampling area was along the Aka Barayun Forest in the Harau Valley. Isolation of fungi was carried out using the multilevel dilution method and poured on PDA medium. The fungus activity test was carried out using Pikovskaya media. Based on the results of the study, 6 isolates were obtained, 3 of which had phosphate solubilizing activity, as indicated by the formation of a clear zone around the fungal colonies. Fungal isolates were measured for colony zone diameter, clear zone diameter, and phosphate solubility index (IKF). The isolate with the highest activity was isolate T1, with an IKF of 1.0. The ability to dissolve phosphate of the fungal isolates obtained was categorized as low.
Isolation of Endophytic Fungi from Trichoma of the Simpei Fern (Cibotium barometz (L.) J. Sm) and Phosphate Solubilizing Activity Test Nia Ramadhanti; Dezi Handayani; Azwir Anhar; Irdawati
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The problem that is often faced by farmers in West Sumatra is the decreasing level of soil fertility which has an impact on crop production due to a lack of phosphate uptake by plants. The problem of phosphate availability can be overcome by utilizing phosphate solubilizing fungi (CPF) because they can be used as biofertilizers. CPF is usually isolated from the soil or rhizosphere, but this fungus is not necessarily compatible with the host plant, so the isolation of endophytic fungi, especially indigenous fungi, is better because it can provide phosphate. CPF can be isolated from plant tissues such as trichomes. The aim of this study was to isolate endophytic fungi from chimpanzee fern trichomes and test their phosphate solubilizing activity. Samples were obtained from Nagari Aka Barayun, Harau District, Fifty Cities District, West Sumatra. Isolation of fungi using Potato Dextrose Agar (PDA) medium, phosphate solvent activity test using Pikovskaya medium. The results showed that 5 isolates of endophytic fungi were successfully isolated from chimpanzee fern trichomes with different macroscopic morphologies: spherical shape, various colors, cotton-like texture, smooth surface. Phosphate solubilizing activity test showed that only ETK 4 isolate had phosphate solubilizing activity with the highest IKF of 0.7.
Isolation of Phosphate Recruiting Endophytic Fungi from the Leaves of the Symphony Fern Plant (Cibotium Barometz (L.) J.Sm) Safira Nurul Fadila; Dezi Handayani; Azwir Anhar; Violita Violita
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Phosphate is one of the elements needed in large quantities for plant growth and development. Phosphate can be found in soil, but generally in the form of bound phosphate, so that it cannot be used by plants. Generally, farmers overcome the availability of phosphate for plants using phosphate fertilizer, but this method is not effective. Another alternative is needed to overcome the availability of phosphate in the soil. One alternative that can be used is to use phosphate-solubilizing endophytic fungi. Phosphate-solubilizing endophytic fungi can be isolated from the leaves of the Simpei fern (Cibotium barometz (L.) J.Sm.). The Simpei fern plant contains organic acids, so endophytic fungi isolated from the leaves of the Simpei fern plant are expected to produce the same organic acids and be able to dissolve phosphate. Therefore, this study aims to determine the phosphate solubilizing activity of the leaves of the Simpei fern plant. The samples used were the leaves of the symbiotic fern. The fungus obtained was tested using Pikovskaya medium. Phosphate solubilizing activity is characterized by the formation of a clear zone around the fungus colony. Six isolates of endophytic fungi from the Simpei fern plant were obtained, namely D1, D2, D3, D4, D5, and D6. Two isolates have phosphate solubilizing activity, namely isolates D2 and D4. The highest phosphate solubility index was shown by isolate D2 on the first day of 0.8. The phosphate solubility index of all isolates is low. Key words: endophytic fungi, fosfat, isolated, activity
Isolasi Cendawan Endofit Pelarut Fosfat dari Akar Tumbuhan Pakis Simpei (Cibotium barometz(L) J.Sm) Celsi Ananda; Dezi Handayani; Azwir Anhar; Violita
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Cendawan pelarut fosfat merupakan cendawan yang mampu mengubah fosfat yang tidak larut menjadi fosfat bebas, Sehingga dapat diserap oleh tanaman dengan mudah. Cendawan pelarut fosfat diisolasi dari tanah atau rizosfer, atau cendawan endofit. Cendawan endofit dapat ditemukan pada akar, batang atau daun tumbuhan. Akar tanaman merupakan habitat yang baik bagi cendawan pelarut fosfat, antara lain akar tumbuhan pakis simpei (Cibotium barometz (L) J.Sm). Tujuan dari penelitian ini adalah untuk mengisolasi cendawan endofit dari akar tumbuhan pakis simpei, dan untuk mengetahui kemampuan melarutkan fosfatnya. Penelitian dilaksanakan di Laboratorium Biologi Umum FMIPA UNP pada bulan Januari hingga Maret 2023. Sampel akar tumbuhan pakis simpei berasal dari Lembah Harau, Kecamatan Harau, Kabupaten Lima Puluh Kota, Sumatera Barat. Potato Dextrose Agar (PDA) digunakan sebagai media isolasi dan Pikovskaya sebagai uji pelarut fosfat. Sterilisasi permukaan menggunakan NaOCl 0,5%. Hasil penelitian menunjukkan bahwa 6 isolat murni cendawan berhasil diisolasi. Terdapat dua isolat yang memiliki aktivitas pelarut fosfat yaitu AK5 dan AK6. Indeks kelarutan terbesar diperoleh pada hari ketiga untuk isolat AK5 dan AK6. Indeks kelarutan adalah 0,2 dan tergolong kategori lemah.
Pelatihan dan Pendirian Bank Sampah Ka Saro untuk Mendukung Pilot Project PHBS TP-PKK Tanjung Balik Azwir Anhar; Mades Fifendy; Eka Vidya Putra; Dwi Hilda Putri; Dezi Handayani; Irma Lailani Eka Putri; Gustina Indriati; Ganda Hijrah Selaras; Siska Alicia Farma
ABDI: Jurnal Pengabdian dan Pemberdayaan Masyarakat Vol 5 No 4 (2023): Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat
Publisher : Labor Jurusan Sosiologi, Fakultas Ilmu Sosial, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/abdi.v5i4.490

Abstract

Masalah persampahan erat kaitannya dengan pertambahan penduduk, pertumbuhan ekonomi dan perubahan pola konsumsi masyarakat. Jumlah penduduk Nagari Tanjung Balik saat ini lebih kurang 2.411 jiwa, sehingga prediksi timbulan sampah adalah sebesar 38,57 m2 per hari. Penyebab utama dari persoalan prioritas nagari ini adalah belum tersedianya TPS dan Bank Sampah di Nagari Tanjung Balik. Tujuan pengabdian ini adalah mendampingi masyarakat dalam membentuk bank sampah, sekaligus menjadi salah satu program unggulan dalam pilot project PHBS Tim Penggerak PKK. Metode yang digunakan dalam kegiatan pengabdian ini adalah metode pendampingan partisipatif. Hasil kegiatan pengabdian berhasil mendirikan bank sampah yang diberi nama Ka Saro. Berdasarkan analisis angket terhadap masyarakat sasaran menunjukkan hasil bahwa terdapat peningkatan pengetahuan, sikap dan keterampilan masyarakat mitra terhadap pengelolaan sampah di Nagarinya. Hasil observasi di lapangan juga menunjukkan antusias yang tinggi dari masyarakat yang menjadi nasabah Bank Sampah Ka Saro. Jumlah nasabah hingga saat ini adalah 100 orang nasabah aktif.
Efektivitas Model Blended Learning Terhadap Hasil Belajar Mahasiswa Pada Mata Kuliah Zoologi di Era Covid-19: (The Effectiveness of the Blended Learning Model on Student Learning Outcomes in Zoology Subjects in the Covid-19 Era) Tomi Apra Santosa; Abdul Razak; Azwir Anhar; Ramadan Sumarmin
BIODIK Vol. 7 No. 01 (2021): March 2021
Publisher : Program Studi Pendidikan Biologi FKIP Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/bio.v7i01.11708

Abstract

The purpose of this study was to determine the differences between the blended learning and offline method models on student learning outcomes in the COVID-19 pandemic. The research used was experimental. The research subjects came from 5-semester biology tadris students of the 2019/2020 academic year IAIN Kerinci. Research instruments in the form of tests and questionnaires via a google form. The data analysis technique was in the form of the t-test with Minitab 19 software. To carry out the hypothesis test, the normality test and the homogeneity test had to be done first. The results showed that the blended learning model was effective in increasing student learning outcomes in the zoology course by an average of 68.16 to 83.42. and provide a significant effect of the blended learning model on student learning outcomes in zoology material. This can be seen from the p-value of 0.000 <0.05. The blended learning model is very good to be applied in schools even in universities where this learning model is able to improve student learning outcomes in the era of the COVID-19 pandemic. Abstrak. Tujuan penelitian ini mengetahui perbedaan  model blended learning dan luring method terhadap hasil belajar biologi siswa pada pandemi COVID-19. Penelitian yang digunakan eksperimen. Subjek penelitian berasal dari mahasiswa tadris biologi semester 5 tahun ajaran 2019/2020 IAIN Kerinci.  Instrumen penelitian berupa tes dan angket melalui google form. Teknis analisis data berupa uji t dengan sofware Minitab 19. Untuk melaksanakan uji hipotesis, maka harus dilakukan dahulu uji normalitas dan uji homegenitas data. Hasil penelitian menunjukan bahwa model blended learning efektif meningkatan hasil belajar mahasiswa pada mata kuliah zoologi dengan rata-rata 68.16 menjadi 83.42. dan  memberikan pengaruh signifikan model blenden learning terhadap hasil belajar mahasiswa pada materi zoologi. Hal dapat dilihat dari nilai p-value 0.000 < 0.05. Model blended learning sangat baik untuk diterapkan di sekolah bahkan di perguruan tinggi dimana model pembelajaran ini mampu meningkatkan hasil belajar mahasiswa di era pendemi covid 19.  
PRODUCTION OF SPESIFIC XYLANASE ENZYME BY MUDIAK SAPAN HOT WATER THERMOPHILIC BACTERY Irdawati Irdawati; Muhammad Deedat Ayasy; Azwir Anhar; Linda Advinda; Yusrizal Yusrizal
Bioscience Vol 8, No 1 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i1.122728

Abstract

Xylanase is an extracellular enzyme capable of hydrolyzing hemicellulose so that it can convert xylan into xylose. Thermostable xylanase enzymes can be produced by thermophilic bacteria. Thermophilic bacteria are used because they have advantages such as the ability to increase enzyme production in adjustable catalytic specifications. Bacteria are not only in a single form but also exist in a mixed form called a consortium. Compared with a single isolate, the performance of the consortium is better. Consortium is a mixture of microbial populations in the form of communities that have cooperative, commensal, and mutualistic relationships. This study aims to look at the cooperation between the consortium isolates and the consortium's ability to produce xylanase enzymes. This research is a descriptive study. The bacterial consortium is fermented in beechwood xylan medium. The results of this study were that MSS 11, MS 18, MS 16 consortium produced the highest xylanase enzyme activity, namely 12,887.Xilanase merupakan enzim ekstraseuler yang mampu menghidrolisis hemiselulosa sehingga dapat merubah xilan menjadi xilosa. Enzim xilanase termostabil dapat diproduksi oleh bakteri termofilik. Bakteri termofilik digunakan karena dapat memiliki keunggulan seperti aktivitas dan peningkatan spesifikasi katalisis yang dapat diatur. bakteri tidak hanya dalam bentuk tunggal namun juga ada dalam bentuk campuran yang disebut konsorsium. Dibandingkan dengan isolat tunggal, kinerja konsorsium lebih baik. Konsorsium merupakan campuran populasi mikroba dalambentuk komunitas yang mempunyai hubungan kooperatif, komensal, dan mutualitsik. Penelitian ini bertujuan untuk melihat kerjasama antar isolat konsorsium dan kemampuan konsorsium dalam menghasilkan enzim xylanase. Penelitian ini bersifat deskriptif. Pada penelitian ini, konsorsium bakteri difermentasikan dalam medium beechwood xylan. Hasil penelitian ini adalah konsorsium MSS 11, MS 18, MS 16 menghasilkan aktivitas enzim xylanase spesifik tertinggi yaitu 12,887.
Floristic composition and economic value of trees at agroforestry parak in Tanjung Raya District, Agam Regency, West Sumatra Indonesia Alfitrah Habibullah. M; Irma Leilani eka Putri; Azwir Anhar; Reki Kardiman
Bioscience Vol 8, No 1 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i1.125863

Abstract

This study aimed to investigate the floristic composition and the economic values of trees at the agroforestry parak in Maninjau, Tanjung Raya District. Four different villages were selected purposively according to the tree structure of parak, and each village represented each compass point surrounding the Maninjau Lake. 20 x 100 m plot was established at each sites, where all tree species were identified, and five owner of the parak at each location were asked about the fruiting and harvesting season, management and potential income from each of tree species. 20 tree species belong to 13 families were found across the sites, but only five were occured at all sites, while the others were missed at least from one site. Most species produced fruits with fruiting and harvesting season ranged from one to three times a year, but only seven species knew the income from selling the commodity. From all seven species, which varied on the harvesting season, the owner of each parak gained up to four million IDR per month. This study showed that variation of species and the harvesting season beneficial the owner of the parak and may kept the parak as primary or alternative livelihood.
The potential of local orange peel-derived eco-enzymes in producing indole acetic acid Siska Alicia Farma; Nurfa Dewiza Luzik; Salma Sakina; Irma Leilani Eka Putri; Linda Advinda; Azwir Anhar
Acta Biochimica Indonesiana Vol. 6 No. 2 (2023): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.135

Abstract

Background: Ecoenzymes, created from the fermentation of organic citrus waste, offer a sustainable method to produce Indole Acetic Acid (IAA), a phytohormone vital for plant growth. This study investigates the potential of these ecoenzymes in promoting sustainable agriculture. Objective: This study aims to evaluate the capacity of ecoenzymes derived from local citrus organic waste to synthesize IAA hormones. Methods: The ecoenzyme was extracted from fruit powders and centrifuged to separate the supernatant. One ml of ecoenzyme supernatant was then mixed with 2 ml of Salkowski reagent and incubated for 12 hours at room temperature in the dark to facilitate reaction. The presence and concentration of IAA were determined using spectrophotometry at a wavelength of 530 nm, while total protein levels were measured using the Warburg-Christian method. Results: ecoenzymes from local citrus sources contain IAA, with the highest concentration observed in sample 7A (30.26 µg/ml). The ecoenzyme exhibited favorable characteristics, including an average degree of acidity of 3.55, and the highest total protein content was found in sample 2A (144.277 mg/mL). Conclusion: Ecoenzymes from local orange peels successfully produce IAA, supported by fermentation-induced microbial activity and acidic conditions. This highlights their potential in sustainable agriculture.
PENGARUH MODEL PEMBELAJARAN PROBLEM BASED LEARNING YANG DI INTERVENSI TEKNOLOGI INFORMASI TERHADAP KETERAMPILAN KOMUNIKASI PESERTA DIDIK Lufri, Lufri; Elmanazifa, Sintia; Anhar, Azwir
Jurnal Pendidikan Biologi Vol 12, No 3 (2021)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um052v12i3p182-186

Abstract

Abstrak. Keterampilan komunikasi perlu dikembangkan dalam proses pembelajran. Namun, proses pembelajaran di sekolah belum menerapkan model pembelajaran yang mengembangkan keterampilan komunikasi peserta didik. Pembelajaran di sekolah masih dominan berpusat pada guru, masih kurang pemanfaatan Teknologi Informasi (TI), tidak aktif, dan kurang minat belajar dikelas. Untuk mengatasi masalah yang dikemukakan tersebut adalah menerapkan model pembelajaran Problem Based Learning(PBL) yang diintervensi TI. Penelitian ini bertujuan untuk meningkatkan keterampilan komunikasi peserta didik. Instrumen yang digunakan berupa rubrik untuk menilai komunikasi yang telah divalidasi oleh pakar. Analisis data yang digunakan yaitu uji Anova dua arah. Hasil penelitian ini menunjukkan perbedaan yang signifikan antara model pembelajaran PBL yang diintervensi teknologi informasi terhadap keterampilan komunikasi peserta didik dengan nilai rata-rata kelas eksperimen 64,27 persen dan kelas kontrol 57,70 persen.Abstract. Communication skills need to be developed in the learning process. However, the learning process in schools has not yet fully implemented a learning model that develops students' communication skills. Learning in schools is still dominantly teacher-centered, there is still a lack of use of Information Technology (IT), not active, and lack of interest in learning in class. To overcome the problems raised by applying the Problem Based Learning (PBL) learning model with IT intervention. This study aims to improve the communication skills of students. The instrument used is a rubric to assess communication that has been validated by experts. Analysis of the data used is a two-way ANOVA test. The results of this study indicate that the communication skills of students in the experimental class (64.27 percent) are significantly higher than the control class (57.70 percent). It can be concluded that problem-based learning that is intervened by information technology has a positive effect on students' communication skills.
Co-Authors Abdul Fatah, Firdaus Abdul Razak Abdul Razak Afyeni, Yutrin Nisya Al Adawiyah Alfitrah Habibullah. M Amrianto Amrianto Amrianto Amrianto, Amrianto Andesmora, Evan Vria Annisa Putri Aslim Rasyad Atiqahh Muthiah Beni Gusnadi Celsi Ananda Chahyunisa, Alifia Desmayanti, Resti Dezi Handayani Diana Susanti Djoelvinanda, Habibullah Dwi Hilda Putri Edwin Musdi Eka Vidya Putra Elmanazifa, Sintia F, Farida Fadhlurrohman, Reza Falta, Lira Andika Farhanah Shofwah Verina Farikh, Muhammad Farma, Sisca Alicia Febri Doni Feby Djumaita Sari Fevria, Resti Fitri, Selfi Kurnia Fitri, Yosi Maidia Fitria, Laili Fitriana Fitriana Gustina Indriati Handayani, Delsi Fitri Handayani, Lena Putri Hapni, Nur Haqil Triyatdipa hilda, Dwi hilda putri Huda Asra, Rahmatul Husnul Khatimah I Made Arnawa I Made Arnawa Ilham Rizky Ritonga Inayatul Fatia Indra Yuzandi Indrawani Matondang IRDAWATI Irdawati Irdawati Irdawati Irdawati Irdawati Irdawati Irma Lailani Eka Putri Irma Leilani Irma Leilani Eka Putri Irma Leilani Eka Putri Iskandar, Ingriani Kardiman, Reki Kartika Sari Khairunisa Khairunisa Kurniasih, Yulvina Laili, Fitria Leilani Eka Putri Lia Angela Linda Advinda Linda Advinda Listi Handayani Lufri Lufri Mades Fifendy Maiyusri Eka Putri Marten, Threo Wanda Masnidar Masnidar Miftahul Jannah Milka Saputri Monica, Della Trya Moralita Chatri Muhammad Deedat Ayasy Mulyadi, Rival Nadira Nadira Nia Ramadhanti Nuari, Sevira Della Nurfa Dewiza Luzik Oktaviani, Mutia Pebriyeni, Silvi Pertiwi, Lastri Pratama, Chelsylia Dara Puspitasari, Winda Putri Erianti Putri, Cici Adelia Putri, Irma Leilani Eka Putri, Lidya Eka Rahmi Septia Sari, Rahmi Septia Rahmi Zahri Zani Rahmi Zahri Zani Ramadan Sumarmin Ramadhan Sumarmin Reki Kardiman Riska Riska Ristiono Ristiono Ristiono Ristiono Roza Yolanda Safira Nurul Fadila Sagurung, Mariance Dartiani Salma Sakina Sari Wirdaningsih Selaras, Ganda Hijrah Selly Fildawati Sepriadi, Ilham Simbolon, Perima Sintia Elmanazifa Siska Alicia Farma Siti Aisyah Sri Rahmadani Fitri Sunarsan, Debra Fortuna Surya Tati Syafni Nola Putri Syahfitri, Aulia Insyani Syahrastani, Syahrastani Syari, Elsy Melia Tarigan, Siti Nadiah Zahra Br Tazri, Mufidah Insani Tiffany Mantoviana Tomi Apra Santosa Santosa Ulfa, Mariyah Valofi, Nagra Aulia Vauzia Vauzia, Vauzia Violita Violita Violita Violita Violita Wibi m Syofian Widya Ruchi Wilny Chintya Wirdaningsih, Sari Wita Ferwati Wulandari, Tesya Yosefin Nisa Aulia Yuni Ahda Yuni Ahda Yusrizal Y Yusrizal Y Yusrizal Yusrizal Zaenab, Nurul Zulyusri Zulyusri Zulyusri, Zulyusri