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Portrait of Hatchery Management Profile on Rainbow Fish Melanotaenia boesemani (Allen & Cross, 1980) Cultivation in Jakarta Area Nugraha, Media Fitri Isma; Avarre, Jean-Christophe; Pouyaud, Laurent; Kadarusman, Kadarusman; Carman, Odang; Junior, M. Zairin
Torani Journal of Fisheries and Marine Science VOLUME 1 NOMOR 2, JUNI 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.153 KB) | DOI: 10.35911/torani.v1i2.4484

Abstract

Hatchery managers and maintaining genetic diversity and fitness population in endangered and threatened species in pond is a important and difficult thing to do.  But is must to do for conservation biology.  Fitness and structure population depends on effective breeding number (Ne) and population connectivity between each other. The second most important thing is gene flow and genetic drift. Ne is major role in the maintenance of genetic diversity as indicator for inbreeding depresion and genetic drift.  We sampled 6 hatchery in Jakarta  and Bekasi Indonesia region and used 12 polymorphic microsatellite loci to quantify population genetic structure. Result in this study are, each farm have different methode. The old farmers is bapak Gusi and bapak Hasan (+30 years).  Ne in bapak Hasan and bapak Gusi hatchery is 66,667 and F value is 0,00749 and loosing allele (P) is 0,26183.  Compared with younger farmers bapak Yahya (5 years) have Ne = 133,333, F = 0,00375 and P = 0,06855.  Even though hatchery bapak Hasan and bapak Gusi have the same value Ne, F and P but the result in population structure they are different founder populations. Hatchery bapak Hasan have unique structure and alleles composition compared with other hatchery.Keywords: breeding, management, Melanotaenia boesemani, hatchery, population.
Induction of reproduction of fish Anasa Nomorhampus sp. endemic Palu, Central Sulawesi orally through hormon bioencapsulation use Chironomus sp. Rezki, Dinda Wahyu; Sudrajat, Agus Oman; Soelistyowati, Dinar Tri; Carman, Odang
Jurnal Akuakultur Indonesia Vol. 24 No. 2 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.2.220-232

Abstract

Species Nomorhampus sp. with the local name Anasa fish, endemic to Palu, Central Sulawesi, has a unique superior mouth shape, beak-shaped jaws, attractive colors and patterns, becoming an export commodity with high economic value, but currently it cannot be cultivated, domestication efforts are needed to avoid extinction, by carrying out hormonal manipulation that accelerates the domestication process. This study aims to evaluate the effectiveness of Oodev® on the induction of reproduction in the fish Nomorhampus sp. through bioencapsulation using Chironomus sp. which resulted in births, five groups of fish were fed using Oodev® at a dose of 1 mL/kg parent weight and NaCl 1 mL/kg parent weight as a control group, the fish were kept for 60 days. This research focuses on assessing specific weight growth rate (LPBS), specific length growth rate (LPPS), survival rate (TKH), gonadosomatic index (IGS), gonadal histology, birth frequency, number of births, and growth performance. Fish fed Oodev® feed showed higher SGR and IGS (p<0.05), 40% of fish fed Oodev® supplementary feed successfully gave birth with a total of 9 fry, while no birth occurred in control fish. Histological analysis showed faster gonad development in fish fed Oodev®. Hormonal induction with Oodev® can accelerate reproduction in anasa fish in cultivation containers. These findings provide valuable insight for fish farmers regarding the effect of Oodev® on gonad development in anasa fish in both male and female parents. It is hoped that this discovery will speed up the process of domestication of Anasa fish. Keywords: domestication, endemic, Nomorhampus sp., Oodev®, reproduction ABSTRAK Spesies Nomorhampus sp. dengan nama lokal ikan Anasa endemik Palu, Sulawesi Tengah, memiliki keunikan bentuk mulut superior rahang berbentuk paruh, warna dan corak menarik menjadi komoditas ekspor dengan nilai ekonomis yang tinggi, namun saat ini belum dapat dibudidayakan, perlu upaya domestikasi agar tidak terjadi kepunahan, dengan melakukan manipulasi hormormonal yang mempercepat proses domestikasi. Penelitian ini bertujuan untuk mengevaluasi efektivitas Oodev® terhadap induksi reproduksi ikan Nomorhampus sp. melalui bioenkapsulasi menggunakan Chironomus sp. yang menghasilkan kelahiran, lima kelompok ikan yang diberi pakan menggunakan Oodev® dosis 1 mL/kg bobot induk dan NaCl 1 mL/kg bobot induk sebagai kelompok kontrol, ikan dipelihara selama 60 hari. Penelitian ini fokus pada penilaian laju pertumbuhan bobot spesifik (LPBS), laju pertumbuhan panjang spesifik (LPPS), tingkat kelangsungan hidup (TKH), indeks gonadosomatik (IGS), histologi gonad, frekuensi kelahiran, jumlah kelahiran, dan kinerja pertumbuhan. Ikan yang diberi pakan Oodev® menunjukkan SGR dan IGS yang lebih tinggi (p<0,05), 40% ikan yang diberi pakan tambahan Oodev® berhasil melahirkan dengan jumlah total 9 ekor benih, sementara pada ikan kontrol tidak terjadi kelahiran. Analisa histologi menunjukkan perkembangan gonad yang berkembang lebih cepat pada ikan yang diberi pakan Oodev®. Induksi hormonal dengan Oodev® mampu mempercepat reproduksi pada ikan anasa di wadah budidaya. Temuan ini memberikan wawasan berharga bagi pembudidaya ikan mengenai pengaruh Oodev® terhadap perkembangan gonad pada ikan anasa baik pada induk jantan maupun betina. Penemuan ini diharapkan dapat mempercepat proses domestikasi ikan Anasa. Kata kunci: domestikasi, endemik, Nomorhampus sp., Oodev®, reproduksi
Effectiveness of Recombinant Growth Hormone on The Growth of Java Barb (Barbonymus gonionotus) with Different Stocking Densities Angraini, Siti Rena Yulia; Alimuddin, Alimuddin; Carman, Odang; Rahmi, Kurnia Anggraini
Jurnal Perikanan Universitas Gadjah Mada Vol 27, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.105229

Abstract

Tawes fish has economic value and affordable price. Growth rate and survival rate are important factors in cultivation productivity. The purpose of this study was to test the effectiveness of recombinant growth hormone of kertang grouper (rElGH) on the growth response of tawes fish seeds at different stocking densities. This study used a factorial randomized design with two treatment factors and three replications. The treatments were rElGH (dose 0 mg/kg and 2 mg/kg) and different stocking densities (40 fish/m3, 80 fish/m3 and 160 fish/m3). The tawes fish seeds used weighed 2.33 ± 0.002 cm and were 4-5 cm long. Maintenance is carried out for 60 days used a hapa measuring 1.0 × 1.0 × 1.0 m3 with a water height of ±60 cm.  Fish are fed with at satiation method using commercial feed with a protein content of 31-33%. The administration of rElGH was carried out on the first 7 days of maintenance and continued with the provision of feed without rElGH. The results of the Two-way ANOVA analysis showed that the use of rElGH and stocking density and their interactions had significant differences. The best treatment was the provision of rElGH stocking density 40 (R40) with survival rate 100%, specific growth rate 3.58%/day, absolute weight gain 8.75 g, and feed conversion ratio 1.12. The combination of the use of rElGH and increasing stocking density significantly increased the biomass production of tawes fish.
The Effect of Recombinant Growth Hormone Administration on Bonylip Barb (Osteochilus vittatus) Fry at Diferent Stocking Densities. Carman, Odang; Maharani, Adinda; Alimuddin; Rahmi, Kurnia Anggraini
Jurnal Media Akuakultur Indonesia Vol 5 No 2 (2025): Indonesian Journal of Aquaculture Medium
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/mediaakuakultur.v5i2.7339

Abstract

The nilem fish (Osteochilus vittatus) has high economic value, but its growth rate is relatively slow. One potential solution to accelerate its cultivation period is the use of recombinant growth hormone (rGH). This study aimed to evaluate the effect of oral administration of rGH derived from Epinephelus lanceolatus (rElGH) on the growth rate of nilem fish juveniles at different stocking densities. A completely randomized design with three replications was used in this study. Juvenile fish with an average length of 4.43±0.03 cm and weight of 0.96±0.03 g were reared in aquaria measuring 100 × 50 × 50 cm with a water height of 20 cm for 30 days. The fish were fed commercial feed containing 31–33% protein, with rElGH-supplemented feed given to satiation twice daily (at 08:00 and 16:00) during the first seven days, followed by regular feed without rElGH. The best production performance was obtained in the R30 treatment group (30 fish per 100 L with rElGH), with an absolute weight gain (AWG) of 3.4 g, a survival rate of 98.89%, and a specific growth rate (SGR) of 4.8% per day, indicating that nilem fish given rElGH grew faster.
Teknis Pengelolaan Pembenihan Ikan Mas Mantap Cyprinus carpio untuk Mendapatkan Benih Kualitas Unggul Iskandar, Andri; Fataya, Salsabilla Galby; Carman, Odang; Ayuningtias, Astri; Juanda, Tatang; Hidayat, Rahmat
Nekton Vol 3 No 2 (2023): October
Publisher : Politeknik Negeri Sambas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47767/nekton.v3i2.497

Abstract

The development of mirror carp (Cyprinus carpio) farming in Indonesia has progressed very rapidly, as can be seen by the increasing number of varieties of mirror carp produced from various regions with their respective specifications and advantages. Along with the need for carp that are more resistant to environmental changes and disease attacks, the development of the Majalaya carp variety subsequently produces Mantap carp fish that are disease-resistant, and growth in the growth segment is faster. The aim of this study was to disseminate technical information on Mantap fish carp culture to the public so that it can be used as a reference source for the community, especially for cultivators who are interested in developing this fish. In this study, primary and secondary data were collected. Based on the results of a fecundity study, one female broodstock produced 104.496 kg broodstock-1, the average percentage of egg fertilization (FR) was 89,64%, the average hatching rate (HR) was 61,37%, and the average survival rate (SR) until the size of the seed size 2-3 cm reaches 60%.
Optimizing goldfish hatchery of the oranda variety Carassius auratus to improve cultivation performance at BSD fish farm Iskandar, Andri; Nurfauzi, Eka Haris; Carman, Odang; Indriastuti, Cecilia Eny; Siregar, Budyansah
Nekton Vol 4 No 2 (2024): October
Publisher : Politeknik Negeri Sambas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47767/nekton.v2i2.760

Abstract

The business of cultivating ornamental fish is quite popular because its sales are profitable for cultivators. Ornamental fish also have attractive colors; therefore, many people are interested in this commodity. One ornamental fish commodity that is in great demand is the oranda goldfish Carassius auratus, which has a variety of colors, unique movements, and body shapes. The annual increase in market demand for oranda goldfish reaches 95,000 fish annually, and each year, there is an increase in the production of 3,000 fish of around 3% each year. This study was carried out in an effort to explore technical information on goldfish cultivation so that the information obtained can be disseminated widely and used as a reference source for the community so that it will add value to the benefit of improving sustainable welfare. The results of the study show that the technique of spawning oranda goldfish can be performed naturally using a substrate in the form of a water hyacinth. The average fecundity produced by one female parent oranda goldfish was 6429 eggs, with a presentation hatching rate of 74% and a presentation survival rate of 64%.
Performance analysis of tetraploid striped catfish (Pangasianodon hypophthalmus) resulting from heat shock induction Hartami, Prama; Carman, Odang; Alimuddin, Alimuddin; Jr, Muhammad Zairin; Rahman, Rahman; Hermawan, Dede
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Jurnal Ilmu Perairan, Vol. 8: No. 1 (April 2021)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v8i1.3828

Abstract

Striped catfish tetraploid induction through heat shock that carried out in this study is not intended to produce on growth fries.But to form a broodstock to produce triploid on growth fries through cross breeding without any physical shock induction process.The fries from the heat shock induction in the previous study were screened or selected by observing the maximum number of nucleoli in fin cells.The number of tetraploid individuals used in the performance test was 105 which were placed in three different concrete tanks.The results showed that the growth of tetraploid striped catfish was more significant than diploid with a difference in the daily weight percentage about 0.497% and 0.158% for length but insignificant differences in FCR and SR.In gonadal development based on histological observations, it was shown that each tetraploid and diploid both male and female striped catfish were at the same level of gonadal maturity.Female gonads were at the previtellogenic oocytes and vitellogenic oocytes, but males are dominated at the stage of spermatocytes and spermatozoa.This histology also shows us that the female gonad was still at the development stage, while the male had entered the mature stage and ready to be spawned.
DIFFERENT LED LIGHT EFFECT ON GONAD MATURATION AND GENE EXPRESSION IN FEMALE SYNODONTIS BROODSTOCKS (Synodontis sp.) Sinansari, Shofihar; Sudrajat, Agus Oman; Arfah, Harton; Alimuddin, Alimuddin; Kusrini, Eni; Carman, Odang
Indonesian Aquaculture Journal Vol 19, No 2 (2024): (December, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.2.2024.179-187

Abstract

The study investigated the influence of the different LED light spectrums on the maturation of the gonads in female synodontis broodstock and examined the maturation-related gene expression levels. Female synodontis were exposed to four different LED light spectra, specifically: white, blue, green, and red, for a continuous period of 120 days rearing. This study employed a completely randomized design, consisting of four treatments and five individual fish as replication for each treatment. An analysis was conducted on the gonadosomatic index (GSI), hepatosomatic index (HSI), gonad histology, estradiol levels, kiss2 and gnrh2 mRNA expression levels. The utilization of blue LED light treatment is highly effective in enhancing the reproductive parameters in female synodontis fish. The results show that brain and gonad kiss2 mRNA expression levels are not significantly different (P<0.05), while gnrh2 mRNA expression levels were significantly different (P<0.05) and had the highest expression in the gonads. The results suggest that light exposure can induce changes in the expression levels of kiss2 and gnrh2 mRNA, as well as control reproduction.
MORPHOMETRIC AND MOLECULAR VARIATION OF NEON TETRA FISH (Paracheirodon innesi) FROM BOJONGSARI DISTRICT, INDONESIA Musthofa, Siti Zuhriyyah; Alimuddin, Alimuddin; Arfah, Harton; Sulistyowati, Dinar Tri; Carman, Odang; Kusrini, Eni
Indonesian Aquaculture Journal Vol 19, No 2 (2024): (December, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.2.2024.133-145

Abstract

Neon tetra, Paracheirodon innesi, is endemic to rivers and streams in southeastern Colombia, eastern Peru, and western Brazil and is commercially traded as aquarium fish in the world. In Indonesia, neon tetras were mass produced in Bojongsari District, Depok, West Java as the centre of neon tetra fish production. Understanding their genetic variation is useful for implementing their selective breeding programs, environmental restoration, and estimating genetic contributions in stocks. The current study aimed to investigate the patterns of morphometric and molecular diversity using randomly amplified polymorphic DNA-polymerase chain reaction techniques among farmed broodstocks of P. innesi in Indonesia. Three populations, namely: the Bojongsari, Curug, and Pondok Petir derived from the Bojongsari District, Depok, west Java, were used in the study. Thirty live fish from each location were analyzed based on 25 truss morphometric characters. Sixty fresh fish samples were obtained for DNA analysis using the RAPD-PCR technique, which uses three random primers. Principal component analysis (PCA) was performed to distinguish morphometric variations among populations. Morphological and molecular analysis displayed a similar result that Bojongsari and Pondok Petir neon tetra fish had high similarities, while Curug neon tetra was distinguished from others. The closest genetic distance was between the Pondok Petir and Curug populations (0.4088), while the farthest genetic distance was between the Curug and Bojongsari populations (0.4138). The results will be useful in developing breeding programs to improve broodstock quality.
INDUKSI HORMONAL PADA SEX REVERSAL UDANG GALAH (Macrobrachium rosenbergii) MENGGUNAKAN EKSTRAK SERBUK SARI PINUS (Pinus tabulaeformis) Arfah, Harton; Soelistyowati, Dinar Tri; Sholihin, Hidayatush; Wibowo, Kesit Tisna; Sudrajat, Agus Oman; Carman, Odang; Maulana, Fajar; Alimuddin, Alimuddin
Jurnal Riset Akuakultur Vol 19, No 2 (2024): Juni (2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.2.2024.85-95

Abstract

Udang galah (Macrobrachium rosenbergii) merupakan komoditas perikanan air tawar yang memiliki ciri dimorfisme seksual pada pertumbuhan individu jantan lebih cepat dibanding betina. Pembentukan populasi monoseks jantan potensial dalam budidaya bertujuan untuk meningkatkan keseragaman, memacu pertumbuhan, dan efisiensi produksi. Induksi sex reversal pada pembentukan individu neomale dapat dilakukan secara hormonal menggunakan bahan yang mengandung derivat testosteron, yaitu serbuk sari pinus (Pinus tabulaeformis). Penelitian ini bertujuan untuk mengevaluasi efektivitas ekstrak serbuk sari pinus terhadap performa sex reversal pada udang galah. Ekstrak serbuk sari pinus dengan dosis berbeda (0,0 mL L-1; 0,2 mL L-1; 0,4 mL L-1; dan 0,6 mL L-1) diberikan melalui perendaman selama 24 jam pada larva udang galah sebelum berdiferensiasi kelamin (PL15) berukuran panjang 12 ± 0,3 mm dan bobot 0,02 ± 0,001 g dengan kepadatan 60 individu per L, kemudian dipelihara selama 45 hari dalam kontainer (60 x 40 x 30 cm3) dengan tinggi air 20 cm dan kepadatan 1 individu per L. Perlakuan dosis ekstrak serbuk sari pinus dosis 0,2 mL L-1 menghasilkan nisbah kelamin jantan 46,69 ± 5,36%, sedangkan peningkatan dosis 0,4-0,6 mL L-1 dan kontrol tidak efektif mengarahkan kelamin jantan (35-42%) dengan tingkat kelangsungan hidup udang PL60 berkisar 66-82%. Ciri kelamin sekunder udang galah jantan PL60 berukuran 30-32 mm berupa male gonopore (MG) dan appendix masculine (AM), sedangkan betina memiliki appendix interna (AI) pada kaki renang kedua dan rongga di antara kedua kaki jalan kelima. Aplikasi ekstrak serbuk sari pinus pada dosis maksimal 0,2 mL L-1 dapat mengarahkan kelamin jantan udang galah.Giant freshwater prawn (Macrobrachium rosenbergii) is a freshwater fishery commodity with sexual dimorphism in which male individuals grow faster than females. The establishment of a male monosexual population is expected to increase individual uniformity, growth rate, and production efficiency. Induction of sex reversal in the formation of the pseudomale can be done hormonally using materials containing testosterone derivatives, for example, pine pollen (Pinus tabulaeformis). This study aimed to evaluate the effectiveness of pine pollen extract on the sex reversal performance of giant prawns. Pine pollen extract with different doses (0.0 mL L-1; 0.2 mL L-1; 0.4 mL L-1; and 0.6 mL L-1) was given by immersion during 24 hours in prawns before sexual differentiation (PL15) with a length of 12 ± 0.3 mm and a weight of 0.02 ± 0.001 g with a density of 60 individuals per L, then reared for 45 days in a container (60 x 40 x 30 cm3) with a water height of 20 cm and a density of 1 individual per L. Treatment with a dose of pine pollen extract at a dose of 0.2 mL L-1 resulted in a male sex ratio of 46.69 ± 5.36%, while increasing doses of 0.4-0.6 ml L-1 and the control were not effective in the sex reversal (35-42%) with a survival rate of PL60 ranged from 66-82%. The secondary sexual characteristics of PL60 male giant prawns measuring 30-32 mm are male gonopore (MG) and masculine appendix (AM), while female giant prawns have an internal appendix (AI) on the second swimming leg and the cavity between the fifth swimming leg. This study concludes that the application of pine pollen extract at a maximum dose of 0.2 mL L-1 could directly induced sex reversal of female to male of giant prawns.
Co-Authors ', Alimuddin , Alimuddin , Rahman, , . Syafiuddin Abinawanto Abinawanto Agus Oman Sudrajat Ahmad Teduh Alimuddin Alimuddin , Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin AM, NM Fatih Asror Amalia Hayati Anang Hari Kristanto Andi Aliah Hidayani Andri Iskandar Angraini, Siti Rena Yulia ANNA OCTAVERA Aulia Saputra Aulia Saputra Avarre, Jean-Christophe Ayi Santika Ayuningtias, Astri Cahyono, Tatak Dwi Chairul Muluk Ching-Fong Chang D.S. Sjafei Dede Hermawan DEDI JUSADI Dendi Hidayatullah, Dendi Deni Radona Deni Radona Deni Radona Deni Radona, Deni Dian Eka Ramadhani Dian Hardiantho Dian R Herdianto Didik Ariyanto Didik Ariyanto Dinar Tri Soelistyowati Dita Puji Laksana Djamhuriyah S. Said Dwi Hany Yanti Eddy Supriyono Elfrida Ratnawati Enang Harris Eni Kusrini Eni Kusrini, Eni Estu Nugroho Eva Ayuzar, Eva Fajar Maulana . Fataya, Salsabilla Galby Fauzan Wahib Alsani Firda Amalia Sukma Firmansyah, Rodhi Flandrianto S. Palimirmo Giri Maruto Darmawangsa GORO YOSHIZAKI Goro Yoshizaki Goro Yoshizaki Guan-Chung Wu Handika Gilang Pramana Putra Harton Arfah Hasan Nasrullah Hendriana, Andri Herjayanto, Muh. Hidayat Hidayat Hylda Khairah Putri, Hylda Khairah I. Andriani I. Herviani Ibrahim Satrio Faqih Ibrahim, Yusran Iis Diatin Indra Lesmana Indriastuti, Cecilia Eny Irin Iriana Kusmini IRMA ANDRIYANI Irvan Faizal Irvan Faizal Irvan Faizal Irwan Irwan Iskandar, Andri Jasmadi Jasmadi Jr, Muhammad Zairin Juanda, Tatang K. Sumantadinata Kadarusman . Kaliky, Nunun Ainun Putri Sari Banun Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Kukuh Adiyana, Kukuh Laurent Pouyaud Lies Emmawati Hadie Lies Emmawati Hadie Lina Mulyani Lola Irma Purwanti M. Rafi M. Syaifudin M. Syukur M. Zairin Junior Maharani, Adinda Marlina Achmad Maskur Maskur Maulana, Fajar MH. Fariduddin Ath-thar Mia Setiawati Moh. Abduh Nurhidayat Mubinun Mubinun Muh. Herjayanto Muhamad Fathurrizki Darmawan Muhamad Syukur MUHAMMAD AGUS SUPRAYUDI Muhammad Fakhri Muhammad Muhammad Muhammad Sami Daryanto Muhammad Zairin Muhammad Zairin Muhammad Zairin Jr Muhammad Zairin Jr. Muslim Muslim Musthofa, Siti Zuhriyyah N. Sugiri Nadia Mega Aryani, Nadia Mega Nasrullah, Hasan Novi Megawati Nugraha, Media Fitri Isma Nur Bambang Priyo Utomo Nurfauzi, Eka Haris Nurjannah, Lilis Prama Hartami Raden Roro Sri Pudji Sinarni Dewi Rahma Vida Anandasari, Rahma Vida Rahman Rahman Rahmat Hidayat Rahmi, Kurnia Anggraini Ratu Siti Aliah Rezki, Dinda Wahyu RIDWAN AFFANDI Rifki Perdian, Muhammad Ronny Rachman Noor Rudhy Gustiano Rudhy Gustiano Ruliaty, Lisa S. Purwati Salamah , Salamah Samalei, Ermianus Sholihin, Hidayatush Sinansari, Shofihar Siregar, Budyansah Siti Subaidah Siti Subaidah Sri Nuryati Sri Setyo Wulandari, Sri Setyo Sri Sundari Subandriyo Subandriyo Sukenda . Sukenda Sukenda Sulistyowati, Dinar Tri Sumie Etoh Syahril, Alfis Toshio Takeuchi Tristiana Yuniarti Urabi, Debby Wartono Hadie Wartono Hadie Wasmen Manalu Wedaraningtyas Nugrahani Wibowo, Kesit Tisna Wicaksono, Aryo Wenang Yogi Himawan Yumaidawati, Nurfitriani Siti Yuni Puji Hastuti Zairin Jr., Muhammad