Claim Missing Document
Check
Articles

Motile Aeromonas Septicemia (MAS) Disease Resistance Test by Aeromonas hydrophila on Triploid Striped Catfish (Pangasianodon hypopthalmus) Prama Hartami; Eva Ayuzar; Salamah Salamah; Lilis Nurjannah; Odang Carman; Alimuddin Alimuddin; Muhammad Rafi; Muhammad Fakhri
Journal of Aquaculture and Fish Health Vol. 13 No. 1 (2024): JAFH Vol. 13 No. 1 February 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i1.45431

Abstract

Motile Aeromonas Septicemia (MAS) disease caused by Aeromonas hydrophila is a common pathogen that attacks freshwater fish commodities, including triploid as the new strain of striped catfish. This study aimed to test the resistance of triploid striped catfish to MAS infection before being released to farmers as a new commodity to increase national striped catfish production. The test was carried out for two months at the Sukamandi Fish Breeding Research Institute, Subang by injecting A. hydrophila into triploid and diploid striped catfish. The challenge test was carried out by injecting the cultured A. hydrophila with a density of 107 cfu.mL-1 as much as 0.1 mL.ind-1 at fish intramuscularly in all treatments, while in the Control (-) treatment, 0.1 mL of PBS solution was injected.tail-1. The test striped catfish used for each treatment were 10 individuals measuring 9.23 – 9.65 cm, and weighing 7.1 – 9.23 g. Survival parameter data and blood description were analyzed quantitatively using Microsoft Excel 2010 and SPSS version 16.0 software with analysis of variance (ANOVA) at a 95% confidence interval. The results of re-characterization with biochemical tests showed that A. hydrophila were gram-negative, oxidase and catalase positive, motile, could fermentatively convert sugar, and could grow in blood media. The survival of triploid striped catfish after the test was 95%, total erythrocytes on day 7, and total leukocytes on the first day were significantly different from diploid striped catfish. Thus, it can be concluded that striped triploid striped catfish are more resistant to infection with A. hydrophila than diploid.
Accelerated of Sex Reversal use 17α-methyltestosterone Induced Female, Orange-Spotted Grouper Epinephelus coioides Odang Carman; Andri Iskandar; Ching-Fong Chang; Guan-Chung Wu; Muslim Muslim; Dian Eka Ramadhani
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i2.40624

Abstract

Highlight Research Sex reversal for orange-spotted grouper Epinephelus coioides The application of 17α-methyltestosterone induce sex change The stability of sex change need more investigation Abstract The occurance of hermaphrodites in grouper fish causes a scarcity of male parents, so an alternative is needed to accelerate sexchange to male at a young age. The present study was expected to scrutinize the mechanisms of sex-change in fish in the early change process, and whether the testis converted from immature ovary using 17α-methyltestosterone (MT) would recover after the termination of MT treatment. MT-induced sex-change and 5-aza-2'-deoxycytidine (5-Aza) were connected as DNA methylation inhibitors to comprehend the alternation of gonadal soma cells. The orange-spotted groupers were used  at the developmental ages and fed a diet containing MT at 50 mg/kg for three months and then a normal diet for a month. In the first week and second week fish injected with 5-Aza intraperitoneally during the MT-oral administration. Most of the fishes in the control group had immature ovaries, but all the females fed with MT, had immature spermatogenesis. However, one month after the withdrawal of MT treatment, the sex of the fish returned to female-like even though the fish have undergone MT-induced masculinization. This outcome demonstrates precocious sex-change from under yearling, orange-spotted grouper utilizing oral MT treatment is impermanent. All the females of 5-aza treatments showed no spermatogenic cells.  In this study, lower growth rates were demonstrated by the MT-treated groups. The impact of this metabolic change was clear after the end of the hormone oral administration since the decreased growth of the groups treated for three months.
INDUKSI HORMONAL PADA SEX REVERSAL UDANG GALAH (Macrobrachium rosenbergii) MENGGUNAKAN EKSTRAK SERBUK SARI PINUS (Pinus tabulaeformis) Harton Arfah; Dinar Tri Soelistyowati; Hidayatush Sholihin; Kesit Tisna Wibowo; Agus Oman Sudrajat; Odang Carman; Fajar Maulana; Alimuddin Alimuddin
Jurnal Riset Akuakultur Vol 19, No 2 (2024): Juni (2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.2.2024.85-95

Abstract

Udang galah (Macrobrachium rosenbergii) merupakan komoditas perikanan air tawar yang memiliki ciri dimorfisme seksual pada pertumbuhan individu jantan lebih cepat dibanding betina. Pembentukan populasi monoseks jantan potensial dalam budidaya bertujuan untuk meningkatkan keseragaman, memacu pertumbuhan, dan efisiensi produksi. Induksi sex reversal pada pembentukan individu neomale dapat dilakukan secara hormonal menggunakan bahan yang mengandung derivat testosteron, yaitu serbuk sari pinus (Pinus tabulaeformis). Penelitian ini bertujuan untuk mengevaluasi efektivitas ekstrak serbuk sari pinus terhadap performa sex reversal pada udang galah. Ekstrak serbuk sari pinus dengan dosis berbeda (0,0 mL L-1; 0,2 mL L-1; 0,4 mL L-1; dan 0,6 mL L-1) diberikan melalui perendaman selama 24 jam pada larva udang galah sebelum berdiferensiasi kelamin (PL15) berukuran panjang 12 ± 0,3 mm dan bobot 0,02 ± 0,001 g dengan kepadatan 60 individu per L, kemudian dipelihara selama 45 hari dalam kontainer (60 x 40 x 30 cm3) dengan tinggi air 20 cm dan kepadatan 1 individu per L. Perlakuan dosis ekstrak serbuk sari pinus dosis 0,2 mL L-1 menghasilkan nisbah kelamin jantan 46,69 ± 5,36%, sedangkan peningkatan dosis 0,4-0,6 mL L-1 dan kontrol tidak efektif mengarahkan kelamin jantan (35-42%) dengan tingkat kelangsungan hidup udang PL60 berkisar 66-82%. Ciri kelamin sekunder udang galah jantan PL60 berukuran 30-32 mm berupa male gonopore (MG) dan appendix masculine (AM), sedangkan betina memiliki appendix interna (AI) pada kaki renang kedua dan rongga di antara kedua kaki jalan kelima. Aplikasi ekstrak serbuk sari pinus pada dosis maksimal 0,2 mL L-1 dapat mengarahkan kelamin jantan udang galah.Giant freshwater prawn (Macrobrachium rosenbergii) is a freshwater fishery commodity with sexual dimorphism in which male individuals grow faster than females. The establishment of a male monosexual population is expected to increase individual uniformity, growth rate, and production efficiency. Induction of sex reversal in the formation of the pseudomale can be done hormonally using materials containing testosterone derivatives, for example, pine pollen (Pinus tabulaeformis). This study aimed to evaluate the effectiveness of pine pollen extract on the sex reversal performance of giant prawns. Pine pollen extract with different doses (0.0 mL L-1; 0.2 mL L-1; 0.4 mL L-1; and 0.6 mL L-1) was given by immersion during 24 hours in prawns before sexual differentiation (PL15) with a length of 12 ± 0.3 mm and a weight of 0.02 ± 0.001 g with a density of 60 individuals per L, then reared for 45 days in a container (60 x 40 x 30 cm3) with a water height of 20 cm and a density of 1 individual per L. Treatment with a dose of pine pollen extract at a dose of 0.2 mL L-1 resulted in a male sex ratio of 46.69 ± 5.36%, while increasing doses of 0.4-0.6 ml L-1 and the control were not effective in the sex reversal (35-42%) with a survival rate of PL60 ranged from 66-82%. The secondary sexual characteristics of PL60 male giant prawns measuring 30-32 mm are male gonopore (MG) and masculine appendix (AM), while female giant prawns have an internal appendix (AI) on the second swimming leg and the cavity between the fifth swimming leg. This study concludes that the application of pine pollen extract at a maximum dose of 0.2 mL L-1 could directly induced sex reversal of female to male of giant prawns.
EFFECT OF A SUPPLEMENTED DIET WITH MELATONIN ON PERFORMANCE OF JAVAEN BARB Systomus orphoides (Valenciennes, 1842) JUVENILE Radona, Deni; Sudrajat, Agus Oman; Alimuddin, Alimuddin; Manalu, Wasmen; Carman, Odang; Dewi, Raden Roro Sri Pudji Sinarni
Indonesian Aquaculture Journal Vol 18, No 2 (2023): (December, 2023)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.18.2.2023.115-122

Abstract

Javaen barb is a native fish in Indonesian inland water with economic value and the potential to be developed as cultured fish resources and ornamental commodities. In the development of aquaculture, there are still problems, such as the low adaptability of the larvae, so that their survival is also low. This study was conducted to evaluate the effect of melatonin supplementation on the productivity of Javaen barb fish juveniles in culture containers. This study was conducted experimentally using a completely randomized design consisting of four treatments with different doses of melatonin supplementation, i.e., A) control (without melatonin supplementation), B) 0.2 mg/100 g of feed, C) 0.4 mg/100 g feed, and D) 0.6 mg/100 g feed, each treatment was repeated three times. Javaen barb juveniles were kept in an aquarium measuring 30×36×60 cm with a water level of 40 cm, consisting of 12 units. Each aquarium was stocked with 150 individuals and given an aeration system with the same air pressure intensity. Feeding was carried out ad-libitum with a frequency of three times daily for 180 days of rearing. The results showed that melatonin supplementation of as much as 0.6 mg/100 g of feed was able to increase the growth of Javaen barb fish juvenile with the highest survival rate (81.33 ± 0.54 %) and feed conversion ratio (2.61 ± 0.14). Melatonin supplementation of 0.6 mg/100 g of feed had higher total leukocyte (3.41±0.73×104 cells/mm3) and hemoglobin (5.07±0.12 g%) values and provided the best production performance in Javaen barb juveniles.
Early Sex Differentiation of Climbing Perch (Anabas testudineus Bloch.): A Pathway to Feminization Hidayat, Rahmat; Carman, Odang; Alimuddin, Alimuddin
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.143-154

Abstract

The phenomenon of sexual dimorphism in climbing perch, which shows that female fish grow faster than males, underlies the development of mono-sex culture. Female mono-sex culture is more applicable for farmers by crossing neo-male fish with normal females. The timing of sexual differentiation in climbing perch is still unknown. It is very useful in sex reversal procedures to produce neo-male climbing perch. This study revealed the time and status of climbing perch sexual differentiation. Ten samples of climbing perch from the spawning of five pairs of parents were taken from the nursery pond at 10–29 days post-hatching (dph). Samples were prepared through a histology preparation procedure. Observations of the structure and characteristics of the gonads were carried out using a light microscope and analyzed histologically. The results indicated that gonad samples aged 10, 11, 12, 13, 15, and 16 dph showed primordial germ cells surrounded by somatic tissue forming genital ridges and mitotic division. Meanwhile, the gonads begin to differentiate as ovaries found at 18 dph with the presence of oogonia and ovarian cavities. Gonads aged 20–21 dph increasingly showed single oogonia cells (size 20–37.5 µm), germ cell cysts, genital ridges, oocytes undergoing the vitellogenesis process, perinucleolar oocytes, and the formation of the ovarian cavity. Sex differentiation of climbing perch was predicted from 18–21 dph. This conclusion underlies that the sex reversal procedure in climbing perch must be carried out before 18 dph.
Effect of Triiodothyronine in Different Dosages on Growth and Survival Rate of Giant Gouramy (Osphronemus gouramy Lac.) Herviani, I.; Junior, M. Zairin; Carman, Odang
Jurnal Akuakultur Indonesia Vol. 2 No. 2 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (376.151 KB) | DOI: 10.19027/jai.2.61-65

Abstract

This experiment was conducted to study the effect of giant gouramy larval immersion in triiodothyronine (T3) hormone solution on their growth and survival rate. One-day old larvae were treated with different concentrations of triiodothyronine (T3) i.e. 0; 0,001; 0,01; 0,1 and 1 ppm by immersion method for 24 hours. During eight weeks rearing period, larvae were fed to satiation with Tubifex three times daily. The media were aerated and changed 10-30% daily. At the end of experiment, there was no significant difference in yolk sac absorption between treated larvae and control. The highest dose of 1 ppm T3 resulted significant decreased in total length, average weight and survival rate of larvae. Key Word : Giant gouramy, Osphronemus gouramy, triiodothyronine, immersion dose, growth, survival rate.   ABSTRACT This experiment was conducted to study the effect of giant gouramy larval immersion in triiodothyronine (T3) hormone solution on their growth and survival rate. One-day old larvae were treated with different concentrations of triiodothyronine (T3) i.e. 0; 0,001; 0,01; 0,1 and 1 ppm by immersion method for 24 hours. During eight weeks rearing period, larvae were fed to satiation with Tubifex three times daily. The media were aerated and changed 10-30% daily. At the end of experiment, there was no significant difference in yolk sac absorption between treated larvae and control. The highest dose of 1 ppm T3 resulted significant decreased in total length, average weight and survival rate of larvae. Key Word : Giant gouramy, Osphronemus gouramy, triiodothyronine, immersion dose, growth, survival rate.
The Caryotipe of Endemic Ornamental Fish Celebes Rainbow (Telmatherina ladigesi) from Maros River, South Sulawesi Andriani, I.; Sugiri, N.; Carman, Odang; Sjafei, D.S.
Jurnal Akuakultur Indonesia Vol. 3 No. 2 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (459.607 KB) | DOI: 10.19027/jai.3.13-18

Abstract

The objective of this research was to find the caryotype of the endemic ornamental fish, Celebes rainbow (Telmatherina ladigesi) from Maros River South Sulawesi, Indonesia. The experimental fish were caught from the branches of Maros River, those are Bantimurung River, Pattunuangasue River and Makkatoang River. Tissue collection and slide preparation were carried out using the solid tissue technique with slight modification. The amount of chromosome pairs found from those three rivers (three population) were 2n = 48. These chromosomes were divided into 13 pairs big chromosomes (>1 urn) and 11 pairs small chromosomes (I u,m) dan 11 pasang kromosom kecil (
Fin Type Variation of Lionhead Strain Goldfish (Carassius auratus) Offspring Syaifudin, M.; Carman, Odang; Sumantadinata, K.
Jurnal Akuakultur Indonesia Vol. 3 No. 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.103 KB) | DOI: 10.19027/jai.3.1-4

Abstract

 Lionhead strain goldfish (Carassius auratus) inheritance produce many variations in phenotype qualitative traits of their offspring that is not common with the parents. Lionhead is an ornamental freshwater fish, they do not have a dorsal fin, but it is a beauty finfish, is popular to the people and have a high economic value. Of the 846 offspring of lionhead is produced in this experiment, and 57,7% of them have dorsal fin (42,3% normal), 13,1% of them have anal fin which did not similar with their parents, 58,6% caudal fin of them did not have similar to their parents. It might be caused by incompletely segregation in meiosis and many gen responsible to certain phenotype trait. Another abnormalities such as no anal fin, blindness, stumped and no pigmen in their gill lamella also occurred. Key words: Inheritance, fin, phenotype, abnormality   ABSTRAK Ikan maskoki strain lionhead menghasilkan keturunan dengan fenotip yang sangat bervariasi dan berbeda dengan induknya. Ikan ini merupakan ikan hias air tawar yang tidak memiliki sirip punggung namun tetap memiliki keindahan, sehingga menjadi begitu populer di masyarakat dan memiliki nilai ekonomi yang tinggi. Sebanyak 846 keturunan (anakan) telah dihasilkan dalam percobaan ini, dan sebanyak 57,7% dari populasi tersebut memiliki sirip punggung (berarti 42,3% merupakan keturunan normal), 13,1% memiliki sirip dubur yang tidak mirip dengan induknya. Variasi keturunan ikan maskoki strain lionhead ini disebabkan oleh segregasi yang tidak sempurna dalam proses meiosis dan banyaknya gen yang terlibat dalam pembentukan penotip tertentu. Abnormalitas lainnya juga terjadi pada keturunan ikan maskoki strain lionhead ini, seperti tidak adanya sirip dubur, mata buta, tubuh pendek dan tidak adanya pigmen pada lemela insang Kata kunci: Keturunan, sirip, fenotip, abnormalitas  
Feminization of Betta Fish (Betta splendens Regan) Through Embryo Immersion in Estradiol-17β Hormone Solution at the Dosages of 400 µg/I for 6, 12, 18 and 24 Hours Purwati, S.; Carman, Odang; Junior, M. Zairin
Jurnal Akuakultur Indonesia Vol. 3 No. 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.233 KB) | DOI: 10.19027/jai.3.9-13

Abstract

The experiment was carried out to study the effect of embryo immersion in estradiol-17β hormone solution on sex ratio, hatching rate and survival rate of betta fish. Eyed embryo were immersed in 400 µg/I estradiol-17β for 0 (control), 6, 12, 18 and 24 hours, and then the larva were reared until reaching three month old. Immersion in estradiol-17β hormone increased percentage in female, and the highest value was achieved at 12 hours of immersion. Increasing treatment duration to 24 hours decreased the survival rate of betta fish. The lowest value of hatching rate was found at 24 hours of immersion. Key words: Feminization, betta fish, betta splendens, sex ratio, survival rate and hatching rate
Effectiveness of B-actin promoter on driving target gene expression in common carp transgenesis Hidayani, Andi Aliah; Carman, Odang; Alimuddin, .
Jurnal Akuakultur Indonesia Vol. 10 No. 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (925.474 KB) | DOI: 10.19027/jai.10.16-23

Abstract

Promoter in transgene construct plays an important role on regulating of transgene expression level in transgenic fish. In fish transgenesis, researcher convinced that use all-fish gene construct is safety and prospective. This study was performed to compare effectiveness b-actin promoter, - the promoter which has ubiquitous, constitutive, housekeeping characteristics, from common carp (homologous) and from tilapia and medaka b-actin promoters (heterologous) in driving of green fluorescent protein (GFP) expression as a model of target gene on common carp transgenesis. These gene constructs were separately microinjected into cytoplasm of 60 one-cell-stage common carp embryos. The results suggested that 70% survival rate at embryo stage and 45% hatching rate values showed that the microinjection was performed successfully. Percentage of embryos expressing GFP gene were slightly higher when injected using common carp and medaka promoters than those of using tilapia promoter. Percentage of larvae expressing GFP using common carp promoter was similar with medaka promoter. Furthermore, GFP expression using common carp b-actin promoter could be detected at one-week-old larvae, while GFP expressing using medaka b-actin promoter was lasted at 2-day-old larvae. The results demonstrated that homologous promoter more effective in driving of a target gene expression than that of heterologous promoter.  Key words: homologous promoter, GFP, transgenesis, common carp   ABSTRAK Promoter dalam konstruksi transgen berperan penting dalam pengaturan tingkat ekspresi transgen pada ikan transgenik. Dalam transgenesis ikan, peneliti meyakini bahwa penggunaan konstruksi gen "all-fish" adalah aman dan prospektif.  Penelitian ini dilakukan untuk membandingkan efektivitas promoter β-aktin, - promoter yang memiliki ciri ubiquitous, constitutive, dan housekeeping, dari ikan dari ikan mas (homolog) dan ikan nila dan ikan medaka (heterolog) dalam mengendalikan ekspresi gen GFP sebagai model gen pada transgenesis ikan mas. Setiap  konstruksi gen tersebut diinjeksikan secara terpisah ke sitoplasma embrio ikan mas fase 1 sel sebanyak 60 embrio. Hasil penelitian dengan kelangsungan hidup embrio 70% dan derajat penetasan 45% menunjukkan bahwa kegiatan mikroinjeksi berhasil dengan baik.  Persentase embrio mengekspresikan gen GFP yang diinjeksi konstruksi gen dengan promoter β-aktin ikan mas dan ikan medaka sedikit lebih tinggi dibandingkan dengan yang menggunakan promoter β-aktin ikan nila.  Selanjutnya, ekspresi gen GFP yang dikendalikan oleh promoter β-aktin ikan mas dapat dideteksi pada larva berumur 1 minggu, sedangkan ekspresi GFP dengan promoter β-aktin ikan medaka hanya bisa terdeteksi hingga larva berumur 2 hari.  Hasil penelitian menunjukkan bahwa promoter homolog adalah lebih efektif dalam mengatur ekspresi gen target dibandingkan dengan promoter heterolog. Kata kunci: promoter homolog, GFP, transgenesis, ikan mas
Co-Authors ', Alimuddin , Alimuddin , Rahman, , . Syafiuddin Abinawanto Abinawanto Agus Oman Sudrajat Ahmad Teduh Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Amalia Hayati Anang Hari Kristanto Andi Aliah Hidayani Andri Iskandar Angraini, Siti Rena Yulia ANNA OCTAVERA Astri Ayuningtias Aulia Saputra Aulia Saputra Avarre, Jean-Christophe Ayi Santika Budyansah Siregar Cahyono, Tatak Dwi Cecilia Eny Indriastuti Chairul Muluk Ching-Fong Chang D.S. Sjafei Dede Hermawan DEDI JUSADI Dendi Hidayatullah, Dendi Deni Radona Deni Radona Deni Radona Deni Radona, Deni Dian Eka Ramadhani Dian R Herdianto Didik Ariyanto Didik Ariyanto Dinar Tri Soelistyowati Dita Puji Laksana Djamhuriyah S. Said Eddy Supriyono Eka Haris Nurfauzi Elfrida Ratnawati Enang Harris Eni Kusrini Estu Nugroho Eva Ayuzar Fajar Maulana . Fauzan Wahib Alsani Firda Amalia Sukma Firmansyah, Rodhi Flandrianto S. Palimirmo Giri Maruto Darmawangsa GORO YOSHIZAKI Goro Yoshizaki Goro Yoshizaki Guan-Chung Wu Handika Gilang Pramana Putra Harton Arfah Hasan Nasrullah Hendriana, Andri Herjayanto, Muh. Hidayat Hidayat Hidayatush Sholihin Hylda Khairah Putri, Hylda Khairah I. Andriani I. Herviani Ibrahim Satrio Faqih Ibrahim, Yusran Iis Diatin Indra Lesmana Irin Iriana Kusmini IRMA ANDRIYANI Irvan Faizal Irvan Faizal Irvan Faizal Irwan Irwan Iskandar, Andri Jasmadi Jasmadi K. Sumantadinata Kadarusman . Kaliky, Nunun Ainun Putri Sari Banun Kesit Tisna Wibowo Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Kukuh Adiyana, Kukuh Laurent Pouyaud Lies Emmawati Hadie Lies Emmawati Hadie Lilis Nurjannah Lina Mulyani Lisa Ruliaty Lola Irma Purwanti M. Syaifudin M. Syukur M. Zairin Junior Maharani, Adinda Marlina Achmad Maskur Maskur MH. Fariduddin Ath-thar Mia Setiawati Moh. Abduh Nurhidayat Mubinun Mubinun Muh. Herjayanto Muhamad Fathurrizki Darmawan Muhamad Syukur Muhammad Agus Muljanto MUHAMMAD AGUS SUPRAYUDI Muhammad Fakhri Muhammad Muhammad Muhammad Rafi Muhammad Sami Daryanto Muhammad Zairin Muhammad Zairin Muhammad Zairin Jr Muhammad Zairin Jr. Muslim Muslim N. Sugiri Nadia Mega Aryani, Nadia Mega NM Fatih Asror AM Novi Megawati Nugraha, Media Fitri Isma Nur Bambang Priyo Utomo Prama Hartami Prama Hartami Raden Roro Sri Pudji Sinarni Dewi Rahma Vida Anandasari, Rahma Vida Rahman Rahman Rahmat Hidayat Rahmat Hidayat Rahmi, Kurnia Anggraini Ratu Siti Aliah Rezki, Dinda Wahyu RIDWAN AFFANDI Ronny Rachman Noor Rudhy Gustiano Rudhy Gustiano S. Purwati Salamah Salamah Salsabilla Galby Fataya Samalei, Ermianus Siti Subaidah Siti Subaidah Sri Nuryati Sri Setyo Wulandari, Sri Setyo Sri Sundari Subandriyo Subandriyo Sukenda . Sukenda Sukenda Sumie Etoh Syahril, Alfis Tatang Juanda Toshio Takeuchi Tristiana Yuniarti Wartono Hadie Wartono Hadie Wasmen Manalu Wedaraningtyas Nugrahani Wicaksono, Aryo Wenang Yogi Himawan Yumaidawati, Nurfitriani Siti Yuni Puji Hastuti Zairin Jr., Muhammad