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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Torani Journal of Fisheries and Marine Science Jurnal Natur Indonesia Widyariset BERITA BIOLOGI Jurnal Akuatika Indonesia Omni-Akuatika Acta Aquatica: Aquatic Sciences Journal Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Medik Veteriner Jurnal Penelitian Perikanan Indonesia Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Techno-Fish Journal of Aquaculture and Fish Health Jurnal Lemuru : Jurnal Ilmu Perikanan dan Kelautan Indonesia Nekton Jurnal Media Akuakultur Indonesia Jurnal Penelitian Belida Indonesia Journal of Fisheries & Marine

Odang Carman

Budidaya Perairan, Institut Pertanian Bogor, Indonesia

Author-ID : 245949

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Social Sciences Veterinary Other

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Early Sex Differentiation of Climbing Perch (Anabas testudineus Bloch.): A Pathway to Feminization Hidayat, Rahmat; Carman, Odang; Alimuddin, Alimuddin
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.143-154

The phenomenon of sexual dimorphism in climbing perch, which shows that female fish grow faster than males, underlies the development of mono-sex culture. Female mono-sex culture is more applicable for farmers by crossing neo-male fish with normal females. The timing of sexual differentiation in climbing perch is still unknown. It is very useful in sex reversal procedures to produce neo-male climbing perch. This study revealed the time and status of climbing perch sexual differentiation. Ten samples of climbing perch from the spawning of five pairs of parents were taken from the nursery pond at 10–29 days post-hatching (dph). Samples were prepared through a histology preparation procedure. Observations of the structure and characteristics of the gonads were carried out using a light microscope and analyzed histologically. The results indicated that gonad samples aged 10, 11, 12, 13, 15, and 16 dph showed primordial germ cells surrounded by somatic tissue forming genital ridges and mitotic division. Meanwhile, the gonads begin to differentiate as ovaries found at 18 dph with the presence of oogonia and ovarian cavities. Gonads aged 20–21 dph increasingly showed single oogonia cells (size 20–37.5 µm), germ cell cysts, genital ridges, oocytes undergoing the vitellogenesis process, perinucleolar oocytes, and the formation of the ovarian cavity. Sex differentiation of climbing perch was predicted from 18–21 dph. This conclusion underlies that the sex reversal procedure in climbing perch must be carried out before 18 dph.

Effect of Triiodothyronine in Different Dosages on Growth and Survival Rate of Giant Gouramy (Osphronemus gouramy Lac.) Herviani, I.; Junior, M. Zairin; Carman, Odang
Jurnal Akuakultur Indonesia Vol. 2 No. 2 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This experiment was conducted to study the effect of giant gouramy larval immersion in triiodothyronine (T3) hormone solution on their growth and survival rate. One-day old larvae were treated with different concentrations of triiodothyronine (T3) i.e. 0; 0,001; 0,01; 0,1 and 1 ppm by immersion method for 24 hours. During eight weeks rearing period, larvae were fed to satiation with Tubifex three times daily. The media were aerated and changed 10-30% daily. At the end of experiment, there was no significant difference in yolk sac absorption between treated larvae and control. The highest dose of 1 ppm T3 resulted significant decreased in total length, average weight and survival rate of larvae. Key Word : Giant gouramy, Osphronemus gouramy, triiodothyronine, immersion dose, growth, survival rate. ABSTRACT This experiment was conducted to study the effect of giant gouramy larval immersion in triiodothyronine (T3) hormone solution on their growth and survival rate. One-day old larvae were treated with different concentrations of triiodothyronine (T3) i.e. 0; 0,001; 0,01; 0,1 and 1 ppm by immersion method for 24 hours. During eight weeks rearing period, larvae were fed to satiation with Tubifex three times daily. The media were aerated and changed 10-30% daily. At the end of experiment, there was no significant difference in yolk sac absorption between treated larvae and control. The highest dose of 1 ppm T3 resulted significant decreased in total length, average weight and survival rate of larvae. Key Word : Giant gouramy, Osphronemus gouramy, triiodothyronine, immersion dose, growth, survival rate.

The Caryotipe of Endemic Ornamental Fish Celebes Rainbow (Telmatherina ladigesi) from Maros River, South Sulawesi Andriani, I.; Sugiri, N.; Carman, Odang; Sjafei, D.S.
Jurnal Akuakultur Indonesia Vol. 3 No. 2 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The objective of this research was to find the caryotype of the endemic ornamental fish, Celebes rainbow (Telmatherina ladigesi) from Maros River South Sulawesi, Indonesia. The experimental fish were caught from the branches of Maros River, those are Bantimurung River, Pattunuangasue River and Makkatoang River. Tissue collection and slide preparation were carried out using the solid tissue technique with slight modification. The amount of chromosome pairs found from those three rivers (three population) were 2n = 48. These chromosomes were divided into 13 pairs big chromosomes (>1 urn) and 11 pairs small chromosomes (I u,m) dan 11 pasang kromosom kecil (

Fin Type Variation of Lionhead Strain Goldfish (Carassius auratus) Offspring Syaifudin, M.; Carman, Odang; Sumantadinata, K.
Jurnal Akuakultur Indonesia Vol. 3 No. 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Lionhead strain goldfish (Carassius auratus) inheritance produce many variations in phenotype qualitative traits of their offspring that is not common with the parents. Lionhead is an ornamental freshwater fish, they do not have a dorsal fin, but it is a beauty finfish, is popular to the people and have a high economic value. Of the 846 offspring of lionhead is produced in this experiment, and 57,7% of them have dorsal fin (42,3% normal), 13,1% of them have anal fin which did not similar with their parents, 58,6% caudal fin of them did not have similar to their parents. It might be caused by incompletely segregation in meiosis and many gen responsible to certain phenotype trait. Another abnormalities such as no anal fin, blindness, stumped and no pigmen in their gill lamella also occurred. Key words: Inheritance, fin, phenotype, abnormality ABSTRAK Ikan maskoki strain lionhead menghasilkan keturunan dengan fenotip yang sangat bervariasi dan berbeda dengan induknya. Ikan ini merupakan ikan hias air tawar yang tidak memiliki sirip punggung namun tetap memiliki keindahan, sehingga menjadi begitu populer di masyarakat dan memiliki nilai ekonomi yang tinggi. Sebanyak 846 keturunan (anakan) telah dihasilkan dalam percobaan ini, dan sebanyak 57,7% dari populasi tersebut memiliki sirip punggung (berarti 42,3% merupakan keturunan normal), 13,1% memiliki sirip dubur yang tidak mirip dengan induknya. Variasi keturunan ikan maskoki strain lionhead ini disebabkan oleh segregasi yang tidak sempurna dalam proses meiosis dan banyaknya gen yang terlibat dalam pembentukan penotip tertentu. Abnormalitas lainnya juga terjadi pada keturunan ikan maskoki strain lionhead ini, seperti tidak adanya sirip dubur, mata buta, tubuh pendek dan tidak adanya pigmen pada lemela insang Kata kunci: Keturunan, sirip, fenotip, abnormalitas

Feminization of Betta Fish (Betta splendens Regan) Through Embryo Immersion in Estradiol-17β Hormone Solution at the Dosages of 400 µg/I for 6, 12, 18 and 24 Hours Purwati, S.; Carman, Odang; Junior, M. Zairin
Jurnal Akuakultur Indonesia Vol. 3 No. 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The experiment was carried out to study the effect of embryo immersion in estradiol-17β hormone solution on sex ratio, hatching rate and survival rate of betta fish. Eyed embryo were immersed in 400 µg/I estradiol-17β for 0 (control), 6, 12, 18 and 24 hours, and then the larva were reared until reaching three month old. Immersion in estradiol-17β hormone increased percentage in female, and the highest value was achieved at 12 hours of immersion. Increasing treatment duration to 24 hours decreased the survival rate of betta fish. The lowest value of hatching rate was found at 24 hours of immersion. Key words: Feminization, betta fish, betta splendens, sex ratio, survival rate and hatching rate

Effectiveness of B-actin promoter on driving target gene expression in common carp transgenesis Hidayani, Andi Aliah; Carman, Odang; Alimuddin, .
Jurnal Akuakultur Indonesia Vol. 10 No. 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Promoter in transgene construct plays an important role on regulating of transgene expression level in transgenic fish. In fish transgenesis, researcher convinced that use all-fish gene construct is safety and prospective. This study was performed to compare effectiveness b-actin promoter, - the promoter which has ubiquitous, constitutive, housekeeping characteristics, from common carp (homologous) and from tilapia and medaka b-actin promoters (heterologous) in driving of green fluorescent protein (GFP) expression as a model of target gene on common carp transgenesis. These gene constructs were separately microinjected into cytoplasm of 60 one-cell-stage common carp embryos. The results suggested that 70% survival rate at embryo stage and 45% hatching rate values showed that the microinjection was performed successfully. Percentage of embryos expressing GFP gene were slightly higher when injected using common carp and medaka promoters than those of using tilapia promoter. Percentage of larvae expressing GFP using common carp promoter was similar with medaka promoter. Furthermore, GFP expression using common carp b-actin promoter could be detected at one-week-old larvae, while GFP expressing using medaka b-actin promoter was lasted at 2-day-old larvae. The results demonstrated that homologous promoter more effective in driving of a target gene expression than that of heterologous promoter. Key words: homologous promoter, GFP, transgenesis, common carp ABSTRAK Promoter dalam konstruksi transgen berperan penting dalam pengaturan tingkat ekspresi transgen pada ikan transgenik. Dalam transgenesis ikan, peneliti meyakini bahwa penggunaan konstruksi gen "all-fish" adalah aman dan prospektif. Penelitian ini dilakukan untuk membandingkan efektivitas promoter β-aktin, - promoter yang memiliki ciri ubiquitous, constitutive, dan housekeeping, dari ikan dari ikan mas (homolog) dan ikan nila dan ikan medaka (heterolog) dalam mengendalikan ekspresi gen GFP sebagai model gen pada transgenesis ikan mas. Setiap konstruksi gen tersebut diinjeksikan secara terpisah ke sitoplasma embrio ikan mas fase 1 sel sebanyak 60 embrio. Hasil penelitian dengan kelangsungan hidup embrio 70% dan derajat penetasan 45% menunjukkan bahwa kegiatan mikroinjeksi berhasil dengan baik. Persentase embrio mengekspresikan gen GFP yang diinjeksi konstruksi gen dengan promoter β-aktin ikan mas dan ikan medaka sedikit lebih tinggi dibandingkan dengan yang menggunakan promoter β-aktin ikan nila. Selanjutnya, ekspresi gen GFP yang dikendalikan oleh promoter β-aktin ikan mas dapat dideteksi pada larva berumur 1 minggu, sedangkan ekspresi GFP dengan promoter β-aktin ikan medaka hanya bisa terdeteksi hingga larva berumur 2 hari. Hasil penelitian menunjukkan bahwa promoter homolog adalah lebih efektif dalam mengatur ekspresi gen target dibandingkan dengan promoter heterolog. Kata kunci: promoter homolog, GFP, transgenesis, ikan mas

Pair replacement on the spawning success of broodstock Seahorse (Hippocampus barbouri) Syafiuddin, .; Junior, M. Zairin; Jusadi, Dedi; Carman, Odang; Affandi, Ridwan
Jurnal Akuakultur Indonesia Vol. 10 No. 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Seahorse, (Hippocampus barbouri) is one of marine living resources having high commercial values and has commonly been traded especially as live ornamental aquarium fish, raw material of traditional medicine and as souvenirs. This expriment was conducted to determine the succces of spawning rate by replacing the broodstock pair of seahorse. This study was done experimentally with treatment of replacement of broodstock pair after spawning under control condition. The experiment was designed to apply completely randomize design by using the following treatments: Treatment A, without replacement neither male nor female. Treatment B, spawned female broodstock was being mated with her unpaired male broodstock. Treatment C, a male broodstock that still brood was being mated with his unpaired female broodstock. Treatment D, a spawned male broodstock that has released larva was being mated with his unpaired female broodstock. Results showed that under control condition the replacement of broodstock pairs of seahorse had significantly influenced the spawning interval, number of eggs released and number of juveniles produced (P0,05). It can be concluded that seahorse is not monogamous, either male or female after being spawned may accept other pair for the next spawning. Key words: pair replacement, broodstock, success spawning, Hippocampus barbouri ABSTRAK Kuda laut, (Hippocampus barbouri) merupakan salah satu sumberdaya hayati laut yang memiliki nilai komersial dan telah banyak diperdagangkan terutama sebagai ikan hias, bahan baku obat tradisional dan juga sebagai suvenir. Penelitian ini dilakukan dengan tujuan untuk mengkaji tingkat keberhasilan pemijahan dengan penggantian pasangan induk kuda laut pada wadah budidaya. Percobaan ini dilakukan secara ekperimental dengan perlakuan penggantian pasangan induk setelah pemijahan dalam wadah budidaya. Percobaan dirancang dengan menggunakan rancangan acak lengkap (RAL) dengan perlakuan sebagai berikut: Perlakuan A, pemijahan sepasang induk kuda laut (tanpa pergantian). Perlakuan B, pemijahan induk betina yang telah memijah dengan induk jantan bukan pasangannya. Perlakuan C, pemijahan induk jantan yang telah memijah (mengerami telur) dengan induk betina bukan pasangannya. Perlakuan D, pemijahan induk jantan yang telah melahirkan dengan induk betina bukan pasangannya. Hasil penelitian menunjukan bahwa penggantian pasangan induk pada wadah budidaya sangat berpengaruh terhadap interval pemijahan, jumlah telur yang dikeluarkan dan jumlah juwana yang dihasilkan (P0,05). Dari hasil penelitian ini disimpulkan bahwa kuda laut, tidak bersifat monogami, artinya baik jantan maupun betina setelah memijah dapat menerima pasangan lain untuk pemijahan berikutnya. Kata kunci: induk, keberhasilan pemijahan, pergantian pasangan, Hippocampus barbouri

Growth and survival of giant gourami juvenile immersed in different doses of recombinant growth hormone Alimuddin, ,; Etoh, Sumie; Pramana Putra, Handika Gilang; Carman, Odang
Jurnal Akuakultur Indonesia Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRAKPenelitian ini dilakukan untuk meningkatkan pertumbuhan benih ikan gurami (Osphronemus goramy) melalui imersi dengan hormon pertumbuhan rekombinan ikan gurami (r-OgGH). Imersi dengan larutan mengandung badan inklusi dilakukan dengan dosis r-OgGH berbeda, yakni 0 mg/L (kontrol), 10 mg/L, 20 mg/L, dan 30 mg/L, sekali seminggu selama tiga minggu. Ikan dipelihara selama lima minggu dalam akuarium dengan kepadatan 5 ekor/L, dan ikan diberi pakan naupli Artemia dan cacing sutera dua kali sehari secara ad libitum. Hasil penelitian menunjukkan bahwa rerata bobot tubuh ikan perlakuan 30 mg/L (0,34 g) adalah sekitar 75% lebih tinggi (p<0,05) daripada perlakuan 10 mg/L (0,24 g) dan kontrol (0,19 g). Kelangsungan hidup tertinggi diperoleh pada perlakuan 30 mg/L (100%), diikuti oleh perlakuan 20 mg/L (96%), perlakuan 10 mg/L (94%), dan kontrol (94%). Dengan demikian, imersi menggunakan hormon pertumbuhan rekombinan dapat digunakan untuk meningkatkan pertumbuhan dan kelangsungan hidup benih ikan gurami.Kata kunci: protein rekombinan, hormon pertumbuhan, imersi, Osphronemus goramy ABSTRACTThis study was aimed at enhancing the growth of giant gourami Osphronemus goramy fry by immersion with recombinant giant gourami growth hormone (r-OgGH). Immersion with solution containing inclusion bodies with different doses of r-OgGH, 0 mg/L (control), 10 mg/L, 20 mg/L, and 30 mg/L was performed on weekly basis for the first three weeks of experimental period. Fish were reared for five weeks in glass aquaria at a stocking density of 7 fish/L, and fed with Artemia nauplii and blood worm twice a day ad libitum. The results showed that the average body weight of 30 mg/L r-OgGH treated fish (0.34 g) was 75% higher (p<0.05) than those of 10 mg/L (0.24 g) and control (0.19 g). The highest survival was obtained at 30 mg/L r-OgGH immersed fish (100%), followed by 20 mg/L (96%) and 10 mg/L treated fish (94%), and control (94%). The results indicate that immersion with recombinant growth hormone could be applied to enhance the growth and survival of giant gourami juvenile.Keywords: recombinant protein, growth hormone, immersion, Osphronemus goramy

Growth of white shrimp post-larvae immersed in recombinant fish growth hormone Laksana, Dita Puji; Subaidah, Siti; Junior, Muhammad Zairin; Alimuddin, ,; Carman, Odang
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2882.889 KB)

ABSTRACT This research was conducted to determine the optimum immersion time of recombinant giant grouper growth hormone (Ephinephelus lanceolatus; rElGH) at a dose of 15 mg/L that generated highest growth of white shrimp post-larvae (PL). PL was bath-immersed for one, two, and three hours. Two types of control was provided, namely it was without any treatment (control), and immersion in water containing 0.01% bovine serum albumin (BSA) and total protein of Escherichia coli without rElGH (pCold control). All treatments and controls were consisted of three replications. A total of 1,500 PL-2 shrimp were bath-immersed in a plastic packing containing 1 L of sea water, 15 mg/L rElGH, and 0.01% BSA. PL was further reared for 21 days in the 60 L glass aquarium, and fed nauplii Artemia two times and flake commercial diet five times daily, at satiation. The results showed that the highest of biomass (36.29±1.46 g), specific growth rate (29.81±0.87%/day), and body length (20.08±0.42 mm) were obtained in three hours immersion treatment (P<0.05). Biomass of PL in three hours immersion treatment was approximately 66.0% higher compared to the control (21.87±2.53 g). Survival of shrimp in all treatment and control were similar (P>0.05). Thus, growth of white shrimp PL could be improved by bath immersion for three hours in rElGH solution of 15 mg/L water. Keywords: recombinant growth hormone, different immersion time, Pacific white shrimp, biomass ABSTRAK Penelitian ini dilakukan untuk menentukan lama waktu perendaman hormon pertumbuhan rekombinan ikan kerapu kertang (Ephinephelus lanceolatus; rElGH) dosis 15 mg/L yang menghasilkan pertumbuhan tertinggi pada pascalarva (PL) udang vaname. Perendaman PL dilakukan satu, dua, dan tiga jam. Dua jenis kontrol dibuat yakni udang vaname PL-2 tidak diberi perlakuan (kontrol), dan direndam dalam air mengandung serum albumin sapi (BSA) 0,01% dan protein Escherichia coli tanpa rElGH (kontrol pCold). Setiap perlakuan dan kontrol diberi tiga ulangan. Sebanyak 1.500 ekor PL-2 direndam dalam kantong plastik kemasan berisi 1 L air laut mengandung rElGH 15 mg/L, dan BSA 0,01%. Selanjutnya, udang dipelihara selama 21 hari di dalam akuarium volume 60 L, dan diberi pakan naupli Artemia sebanyak dua kali dan pakan komersial berbentuk flake sebanyak lima kali sehari hingga kenyang. Hasil penelitian menunjukkan bahwa biomassa (36,29±1,46 g), pertumbuhan bobot spesifik (29,81±0,87%/hari), dan panjang tubuh (20,08±0,42 mm) tertinggi diperoleh pada perlakuan perendaman selama tiga jam (P<0,05). Biomassa udang perlakuan perendaman selama tiga jam lebih tinggi 66% dibandingkan dengan kontrol (21,87±2,53 g). Kelangsungan hidup udang yang direndam dengan rElGH, kontrol dan kontrol pCold tidak berbeda nyata (P<0,05). Dengan demikian, pertumbuhan PL udang vaname dapat ditingkatkan melalui perendaman selama tiga jam dalam larutan rElGH 15 mg/L air. Kata kunci: hormon pertumbuhan rekombinan, lama perendaman, pascalarva udang vaname, biomassa

Testicular cell transplantation of neon tetra Paracheirodon innesi into common carp fry Alimuddin, ,; Carman, Odang; Wulandari, Sri Setyo
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Neon tetra Paracheirodon innesi is an ornamental fish that have high export value. However, production is still relatively low due to low fecundity (approximately 180 eggs/female). Technology of testicular cell transplantation of neon tetra as donor to common carp as recipient fish which have high fecundity provides a promising way to overcome problem of neon tetra production. This research was performed to determine the optimum age of common carp fry that is able to receive donor cells and allow high success of transplantation. In this research, the testes of neon tetra fish were dissociated by 0.5% trypsin solution. The testicular cells were labeled with PKH-26 fluorescent dye, and then transplanted into the peritoneal cavity of seven, ten, and 14 days post hatching common carp fry. The results showed that the survival of seven day-old transplanted fry (31.25%) was lower than that of ten day-old (37.75%) and 14 day-old transplanted fry (56.25%). Percentage of fish colonized testicular cells donor at 21 days post-transplantation on seven days old and ten days old fry were similar (80%), while on 14 day-old fry was 60%. Based on the cumulative transplantation success rate (survival and colonization rates), transplantation on 14 days old fry (33.75%) showed higher result compared to transplantation on seven days old fry (25.00%) and ten day-old fry (30.00%). It can be concluded that transplantation of neon tetra testicular cells to common carp fry have been successfully carried out, and the optimum age of common carp fry to transplantation was 14 days after hatching. Keywords: transplantation, colonization, testicular cells, common carp, neon tetra ABSTRAK Ikan neon tetra Paracheirodon innesi merupakan ikan hias yang memiliki nilai ekspor yang tinggi. Namun demikian, tingkat produksinya masih relatif rendah karena fekunditas ikan neon tetra yang sedikit (sekitar 180 telur/induk). Teknologi transplantasi sel testikular ikan neon tetra (ikan donor) ke ikan mas yang memiliki fekunditas telur yang banyak dan diharapkan mampu mengatasi ketersediaan benih ikan neon tetra. Penelitian ini bertujuan untuk menentukan umur optimum benih ikan mas (calon ikan semang) yang mampu menerima sel donor dengan baik dan memiliki keberhasilan kolonisasi yang tinggi. Testis ikan neon tetra didisosiasi menggunakan larutan tripsin 0,5%. Sel testikular diwarnai dengan PKH-26, kemudian ditransplantasikan ke rongga peritoneal benih ikan mas umur tujuh, sepuluh, dan 14 hari setelah menetas. Hasil penelitian menunjukkan bahwa tingkat kelangsungan hidup ikan mas perlakuan transplantasi umur tujuh hari (31,25%) lebih rendah dibandingkan dengan perlakuan transplantasi umur sepuluh hari (37,50%) dan 14 hari (56,25%). Persentase ikan terkolonisasi sel donor pada hari ke-21 pascatransplantasi pada benih umur tujuh dan sepuluh hari adalah sama (80%), sedangkan transplantasi benih umur 14 hari sebesar 60%. Berdasarkan keberhasilan transplantasi secara kumulatif (tingkat kelangsungan hidup dan kolonisasi), transplantasi pada benih umur 14 hari (33,75%) menunjukkan hasil lebih tinggi dibandingkan dengan perlakuan transplantasi pada benih umur tujuh hari (25,00%) dan benih umur sepuluh hari (30,00%). Transplantasi sel testikular ikan neon tetra pada benih ikan mas telah berhasil dilakukan, dan umur optimum benih ikan mas adalah 14 hari setelah menetas. Kata kunci: transplantasi, kolonisasi, sel testikular, ikan mas, ikan neon tetra

Co-Authors ', Alimuddin , Alimuddin , Rahman, , . Syafiuddin Abinawanto Abinawanto Agus Oman Sudrajat Ahmad Teduh Alimuddin Alimuddin , Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin AM, NM Fatih Asror Amalia Hayati Anang Hari Kristanto Andi Aliah Hidayani Andri Iskandar Angraini, Siti Rena Yulia ANNA OCTAVERA Aulia Saputra Aulia Saputra Avarre, Jean-Christophe Ayi Santika Ayuningtias, Astri Cahyono, Tatak Dwi Chairul Muluk Ching-Fong Chang D.S. Sjafei Dede Hermawan DEDI JUSADI Dendi Hidayatullah, Dendi Deni Radona Deni Radona Deni Radona Deni Radona, Deni Dian Eka Ramadhani Dian R Herdianto Didik Ariyanto Didik Ariyanto Dinar Tri Soelistyowati Dita Puji Laksana Djamhuriyah S. Said Eddy Supriyono Elfrida Ratnawati Enang Harris Eni Kusrini Eni Kusrini, Eni Estu Nugroho Eva Ayuzar, Eva Fajar Maulana . Fataya, Salsabilla Galby Fauzan Wahib Alsani Firda Amalia Sukma Firmansyah, Rodhi Flandrianto S. Palimirmo Giri Maruto Darmawangsa GORO YOSHIZAKI Goro Yoshizaki Goro Yoshizaki Guan-Chung Wu Handika Gilang Pramana Putra Harton Arfah Hasan Nasrullah Hendriana, Andri Herjayanto, Muh. Hidayat Hidayat Hylda Khairah Putri, Hylda Khairah I. Andriani I. Herviani Ibrahim Satrio Faqih Ibrahim, Yusran Iis Diatin Indra Lesmana Indriastuti, Cecilia Eny Irin Iriana Kusmini IRMA ANDRIYANI Irvan Faizal Irvan Faizal Irvan Faizal Irwan Irwan Iskandar, Andri Jasmadi Jasmadi Jr, Muhammad Zairin Juanda, Tatang K. Sumantadinata Kadarusman . Kaliky, Nunun Ainun Putri Sari Banun Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Kukuh Adiyana, Kukuh Laurent Pouyaud Lies Emmawati Hadie Lies Emmawati Hadie Lina Mulyani Lola Irma Purwanti M. Rafi M. Syaifudin M. Syukur M. Zairin Junior Maharani, Adinda Marlina Achmad Maskur Maskur Maulana, Fajar MH. Fariduddin Ath-thar Mia Setiawati Moh. Abduh Nurhidayat Mubinun Mubinun Muh. Herjayanto Muhamad Fathurrizki Darmawan Muhamad Syukur MUHAMMAD AGUS SUPRAYUDI Muhammad Fakhri Muhammad Muhammad Muhammad Sami Daryanto Muhammad Zairin Muhammad Zairin Muhammad Zairin Jr Muhammad Zairin Jr. Muslim Muslim Musthofa, Siti Zuhriyyah N. Sugiri Nadia Mega Aryani, Nadia Mega Novi Megawati Nugraha, Media Fitri Isma Nur Bambang Priyo Utomo Nurfauzi, Eka Haris Nurjannah, Lilis Prama Hartami Raden Roro Sri Pudji Sinarni Dewi Rahma Vida Anandasari, Rahma Vida Rahman Rahman Rahmat Hidayat Rahmi, Kurnia Anggraini Ratu Siti Aliah Rezki, Dinda Wahyu RIDWAN AFFANDI Ronny Rachman Noor Rudhy Gustiano Rudhy Gustiano Ruliaty, Lisa S. Purwati Salamah , Salamah Samalei, Ermianus Sholihin, Hidayatush Sinansari, Shofihar Siregar, Budyansah Siti Subaidah Siti Subaidah Sri Nuryati Sri Setyo Wulandari, Sri Setyo Sri Sundari Subandriyo Subandriyo Sukenda . Sukenda Sukenda Sulistyowati, Dinar Tri Sumie Etoh Syahril, Alfis Toshio Takeuchi Tristiana Yuniarti Wartono Hadie Wartono Hadie Wasmen Manalu Wedaraningtyas Nugrahani Wibowo, Kesit Tisna Wicaksono, Aryo Wenang Yogi Himawan Yumaidawati, Nurfitriani Siti Yuni Puji Hastuti Zairin Jr., Muhammad

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