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All Journal Jurnal Gizi dan Pangan Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Pengolahan Hasil Perikanan Indonesia Hemera Zoa Jurnal Sain Veteriner Jurnal Ilmu Komputer dan Informasi Jurnal Veteriner Majalah Kedokteran Bandung Jurnal Ilmu Ternak Veteriner Jurnal Media Gizi Indonesia (MGI) INDONESIAN JOURNAL OF PHARMACY Pharmascience Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry Jurnal Medik Veteriner Amerta Nutrition Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Farmamedika (Pharmamedica Journal) Jurnal Dunia Gizi Narra J Journal of Health and Nutrition Research Journal of Global Pharma Technology Jurnal Kedokteran Hewan Current Biomedicine Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research)

Ekowati Handharyani

Departemen Klinik Reproduksi Patologi, Fakultas Kedokteran Hewan, IPB University, Kampus IPB Dramaga Bogor, 16680, Indonesia

Author-ID : 274303

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Environmental Science Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Perubahan patologis dan gambaran lekosit pada itik yang diinfeksi Pasteurella multocida Wiwin Winarsih; Ekowati Handharyani; Agus Setiyono
Hemera Zoa Vol. 77 No. 1 (1995): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Enam puluh ekor itik yang berumur lima minggu dipergunakan dalam penelitian ini. ltik dibagi secara acak menjadi dua kelompok yaitu kelompok infeksi dan kontrol.Kelompok pertama diinfeksi dengan Pasteurelhz multocida (Isolat lapangan) secara intramuskular. Itik diambil darahnya dan dinekropsi 1, 2, 4 dan 8 jam setelah infeksi untuk pemeriksaan diferensial lekosit dan perubahan makroskopik.Itik yang diinfeksi mulai menunjukkan lesio empat jam setelah infeksi. Itik yang diinfeksi dan mati menunjukkan gejala septikemi dengan hiperemi umum. Pemeriksaan gambaran lekosit menunjukkan peningkatan jumlah heterofil dan penurunan jumlah limfosit dan monosit 2,4 dan 8 jam setelah infeksi.

Perbandingan pengaruh Escherichia coli dan vaksinasi ND La Sota terhadap bursa fabricus anak ayam pedaging Ekowati Handharyani; Hernomoadi .; Bibiana W. Lay; M.B. Malole .
Hemera Zoa Vol. 77 No. 2 (1995): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Infeksi E. coli pada anak ayam pedaging secara mandiri dapat menimbulkan penurunan jumlah sel limfosit (24.657%). radang interstisial (15.330%), hiperemi (24.811%). serta penurunan berat relatif bursa Fabricius (0.152 gram persen). Vaksinasi ND La Sota (tetes mata). dilanjutkan dengan infeksi E. coli menyebabkan penurunan jumlah sel limfosit (7.314%), edema radang (3.826%). hiperemi (9.569%) dan penurunan berat relatif bursa (0.150 gram persen). Pemberian vaksin ND La Sota (tetes mata) menyebabkan perubahan yang minimal pada bursa Fabricius berupa penurunan jumlah sel limfosit (2.03 1 %), edema (2.348%). hiperemi (2.03 1 %).

Studi Beberapa Penyakit yang ditemukan bersama dengan gumboro/infectious bursal disease Ekowati Handharyani; Hernomoadi Hernomoadi
Hemera Zoa Vol. 75 No. 3 (1992): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Pengamatan terhadap 43 kasus Gumboro di Laboratorium Patologi Unggas FKH IPB pada tahun 1991 menunjukkan bahwa penyakit Gumboro dapat terjadi pada ayam pedaging maupun ayam petelur dengan kisaran umur 7-60 hari. Kehadiran Gumboro daapt menyebabkan penurunan fungsi tanggap kebal sehingga ayam lebih peka terhadap infeksi penyakit lain. Hasil kajian yang diperoleh adalah: Gumboro dapat berdiri sendiri (30.23%) atau disertai penyakit lain; seperti ND (30.23%), CRD (9.30%), Coccidiosis (6.98%), Colibacillosis (4.67%), ND dan CRD (13.95%), ND dan Coccidiosis (2.32%), CRD dan Coccidiosis (2.32%).

MP-8 Lack of Antibody Formation Against Inactivated Avian Influenza Virus in Ducks and Chickens After Intranasally Immunization Okti Nadia Poetri; Retno Damayanti Soejoedodo; Ni Luh Putu Ika Mayasari; Ekowati Handharyani; Novera Nirmalasanti
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Vaccination is one of control strategies implemented in endemic countries such as Egypt and Indonesia [1,2]. Most commercial AI vaccines available in Indonesia are adjuvanted inactivated AI vaccines applied through intramuscular routes. Vaccine application by subcutaneous or intramuscular injection can cause pain and stress in poultry, the route of vaccine through the nasal drip (intranasal) is a more convenient and painless. However, respiratory applied inactivated influenza is poorly immunogenic. Therefore prior to developing inactivated intranasal vaccine, it is necessary to study antibody response to inactivated AI virus which exposed through intranasal route. The aim of our research was to determined antibody response of ducks and chickens against avian influenza virus (AIV) subtype H5N1 after intranasally immunization.

PAT-7 A Natural Case Like Diabetes Rat as A New Approach on Understanding Alert of Glucose Methabolism Adi Winarto; Komariah Komariah; Kiranadi Bambang; Manalu Wasmen; Ekowati Handharyani
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Currently, diabetic case as a degenerative disease has widely known that it can be cure by insulin. A remain puzzle is proliferation do not found in rest Beta cells, so insulin injection has to be applied entire life of diabetic patient. So far, there is no diagnostic approach that expresses such of thing which may indicate an early anomaly on glucose metabolism process. The aim of this research to get case model of hyperglycemic rat that has long period in process.

DIAGNOSIS GANGGUAN SISTEM URINARI PADA ANJING DAN KUCING MENGGUNAKAN VFI 5 Agus Buono; Dhany Nugraha Ramdhany; Aziz Kustiyo; Ekowati Handharyani
Jurnal Ilmu Komputer dan Informasi Vol 2, No 2 (2009): Jurnal Ilmu Komputer dan Informasi (Journal of Computer Science and Information)
Publisher : Faculty of Computer Science - Universitas Indonesia

Sistem urinari hewan dapat dibagi menjadi 2 bagian yaitu sistem urinari bagian atas dan sistem urinari bagian bawah. Ginjal yang merupakan bagian dari sistem urinari memiliki 2 fungsi penting, yaitu filtrasi dan reabsorpsi. Dalam mendiagnosis penyakit yang diderita hewan pada sistem urinarinya terdapat beberapa kendala. Pada penelitian ini, dikembangkan model untuk mendiagnosis gangguan sistem urinari pada anjing dan kucing dengan menggunakan algoritma VFI 5 berdasarkan gejala klinis (terdapat 37 feature) dan pemeriksaan laboratorium (39 feature). Percobaan dilakukan baik pada feature gejala klinis dan juga pada feature pemeriksaan laboratorium. Hasil pengamatan yang dilakukan menunjukkan bahwa akurasi rata-rata sebesar 77,38% untuk percobaan dengan feature gejala klinis, dan 86,31% untuk percobaan dengan feature pemeriksaan laboratorium. Peningkatan ini mengindikasikan bahwa dalam mendiagnosis penyakit dalam sistem urinari, pemeriksaan laboratorium masih sangat dibutuhkan dalam menentukan hasil diagnosis suatu penyakit.

Seroprevalence of Q fever in sheep and goat in West Java area Agus Setiyono; Ekowati Handharyani; Hapsari Mahatmi
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Q fever is a zoonotic disease caused by Coxiella burnetii, a species of bacteria that is distributed globally. Ruminant especially sheep and goats may play an important role in the transmission of the disease to human. The research of seroprevalence of Q fever in sheep and goats was carried out from August 2006 to March 2007 in West Java area. A total of 138 sera were collected; 69 sera from sheep and 69 sera from goats. The indirect immunoflourescent antibody test was used to determine the seroprevalence of Q fever. The seropositive based on the dilution of serum starting from 1 : 16. Seropositive were observed in 22 samples (31.88%) of sheep and 14 samples (20.28%) of goats. The highest titer of 1 : 128 was observed in 3 pregnant sheep. The results of the present study suggested that Q fever was endemic in West Java area. Key Words: Q Fever, Prevalence, Indirect Immunoflourescent Antibody Test

Extrahepatic bile duct ligation in broiler chickens: ultrastructural study of Ito cell Ekowati Handharyani; K Ochiai; A Winarto
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 4 (2004): DECEMBER 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The Ito cell (fat-storing cell) is a cell lying in perisinusoidal space of liver. The function of Ito cell is expanding from a site of fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. This study was performed in order to evaluate the Ito cells in cholestatic condition. The artificial cholestatic was conducted by ligation of extrahepatic bile ducts (bile duct ligation = BDL) in broilers. The results showed that BDL induced bile congestion, fibrosis, proliferation of Ito cells and intrahepatic bile ductules. Immunohistochemistry demonstrated that Ito cells were scattered throughout the fibrotic areas, and larger in size with more extensive immunoreactivity than those in normal livers. Ultrastructural study demonstrated that Ito cells were closely associated with the production of extracellular collagen fibers. Ito cells actively react against hepatocytic injuries, especially in fibrogenesis of cholestatic livers. Key words: Bile duct, ito cell, broiler

Deteksi Coxiella burnetii pada Sapi Kurban Idhul Adha di Cimanggu, Kabupaten Bogor Tahun 2015-2016 (DETECTION OF COXIELLA BURNETII IN IED AL-ADHA SACRIFICIAL COW IN CIMANGGU, BOGOR REGENCY AT 2015-2016) Handayu Untari; Agus Setiyono; Ekowati Handharyani; Masdiana C. Padaga
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Coxiella burnetii is an intracellular obligate bacterium that causes Q fever disease. Q fever is one of the asymptomatic zoonosis and has a potential as biological weapon. This disease infects much type of animals and has been distributed globally, including in Indonesia. Previous research concluded that West Java is an endemic area of this disease. Bogor in West Java has a potential spread of Q fever disease due to high population of cattle and goat in this region.This study aim was to detect C. burnetii in spleen, lung, kidney, liver and heart of cows. Samples of were taken from a total of 29 sacrificial cows during Eid al-Adha in 2015 and 2016 in the Cimanggu region, Bogor Regency. The examination method used was immunohistochemistry with primary polyclonal antibody Rabbit anti-C. burnetii and HaematoxylineEosine staining. The results of the immunohistochemistry examination showed positive immunoreaction (specific brown color in the cytoplasm of cells) against C. burnetii antibodies respectively in 3 out of 10 samples in 2015 (30%) and 4 out of 19 samples in 2016 (21%). Out of 29 samples, positive immunoreaction were found in lung organ (13,7%) and spleen (10,3%) with variation in changes in histopathological features which include congestion, inflammatory cell infiltration, mucus accumulation, and fibrosis in the lung.

Deteksi Virus Avian Influenza H5N1 pada Anak Ayam Umur Satu Hari dengan Teknik Imunohistokimia Sophia Setyawati; Retno Damajanti Soejoedono; Ekowati Handharyani; Bambang Sumiarto
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Avian Influenza (AI) or bird flu caused by virus H5N1 is still present in Indonesia. The Department ofAgriculture of Indonesia has banned poultry distribution from endemic to non endemic area, except fordistribution of day old chick (DOC). The aim of this research was to detect AI virus infection in DOCdistributed from AI endemic to AI non endemic areas. Two hundred and forty DOCs from farms in WestJava and Banten were collected from Soekarno Hatta airport. Their antibody titers were examined againstAI virus by Haemaglutination Inhibition (HI) test. The AI virus detected in tissues (trachea, lung, heart,kidney, liver, and intestine) by immunohistochemistry technique. Detection of AI virus using anti AI H5N1monoclonal antibody was conducted AEC as chromogen. The result showed that 66,2% of DOC were positiveAI and 33,8% were negative AI. The 66,2% of positive samples, 43,3% showing the presence of AI antigenin trachea, lung and intestine, and 22,9% were presence in liver and kidney. DOCs were infected AI viruswith subclinical symptoms and they were potential as the source of rapid AI spread in Indonesia. It istherefore important to take a very cautious measure to prevent the spread of AI via DOC from AI endemicto free area.

Co-Authors ., Chairul A Winarto Adi Winanto Adi Winarto Afiff , Usamah Agus Buono Agus Setiyono Ahmad Biharidin Ahmad Sulaeman Akhmad Endang Zainal Hasan Aldo Yanuar Wuriyantara Armenia Eka Putriana Arni Diana Fitri Aulia Andi Mustika Aziz Kustiyo Bambang Kiranadi Bambang Sumiarto Bambang Widyo Prastowo BIBIANA W LAY Budiono, Novericko Ginger Chairul - Chairul Chairul Clara Meliyanti Kusharto Deni Noviana Denny Widaya Lukman Dewa, Wisnu Jaka DEWI APRI ASTUTI Dewi Ratih Agungpriyono Dewi Ulfa Trisdiani Dhany Nugraha Ramdhany Dondin Sajuthi Dwi Santy Damayati Dwi Utari Rahmiati Eflisa Endah Putri Elly Munadziroh Eny Palupi Erni Sulistiawati Erwin Erwin Etriwati E Evy Damayanthi Fadjar Satrija Fajar Kawitan Firmansyah, Hardi Fitri Ariyani Fitri, Arni Diana Franciscus Dhyana Giri Suyatna Geneva Ariesta Giriwono, Puspo E. Gunanti . Hadi Riyadi Handayu Untari Hapsari Mahatmi Hardi Firmansyah HERA MAHESHWARI Hernomoadi . Hernomoadi Hernomoadi Herwin Pisestyani Hidayah Dwiyanti Huda Shalahudin Darusman I wayan Teguh Wibawan Ikeu Ekayanti Irma Herawati Suparto K Ochiai Karsi Ambarwati Katrin Roosita KHOIRIYAH, ROMYUN ALVY Kiranadi Bambang Komariah Komariah Komariah Lili Andriani Lina Noviyanti Sutardi M.B. Malole . MARIA BINTANG Masdiana C. Padaga Masriani . Nabilah, Avida Shahnaz Nasution, Zuraidah Ni Luh Putu Ika Mayasari Nisrina Rosyida Noor Rifai Novera Nirmalasanti Nugraha, Arifin Budiman Nugroho, Setyo Widi Octarina Okti Nadia Poetri Olga Purnama Bakti Opyn Mananta Perdhana, Ika Satya Putra Wijaya, Erlangga Putri Puncak Anjani R. Susanti Rahayu WoroWiranti Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Setyaningsih Retno Setyaningsih Rimbawan , Rimbawan Rimbawan Risti Rosmiati Riyadh Firdaus Rizal M Damanik, Rizal M Ruqiah Ganda Putri Panjaitan safrina dyah hardiningtyas Sangko Sayuti Nasution Sangko Sayuti Nasution, Sangko SEDARNAWATI YASNI SILMI MARIYA Sophia Setyawati Sri Anna Marliyati sri murtini . Sri Purwaningsih Sri Rahmatul Laila Sri Wahyuni Suhendi, Adnan Rizal Surachmi Setiyaningsih Tanjung Penataseputro Tantri, Aida Rosita Trini Suryowati, Trini Umi Cahyaningsih Wasmen Manalu Wibowo, Artdhea Regita Wiwin Winarsih Yetty Ramli Yuli Purwandari Kristiangingrum Yuni Andriani Yusuf Ridwan Zulfa Zakiah

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