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Pengaruh Pemberian Ekstrak Etanol Keong Matah Merah (Cerithidea obstusa) Terhadap Kadar Enzim Transaminase Tikus Putih (Rattus novergicus) Dewi Ulfa Trisdiani; Sri Purwaningsih; Ekowati Handharyani
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 17, No 2 (2022): Desember 2022
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v17i2.853

Keong matah merah (Cerithidea obtusa) terbukti memiliki potensi farmakologis sebagai obat. Penelitian ini bertujuan menganalisis kadar enzim transaminase, yaitu serum glutamic pyruvic transaminase (SGPT) dan serum glutamic oxaloacetic transaminase (SGOT) pada serum tikus putih (Rattus novergicus) jantan galur Sprague Dawley yang diberi ekstrak etanol daging keong matah merah (C. obtusa) selama 28 hari. Kadar SGOT dan SGPT yang mengalami peningkatan secara bersamaan dapat menjadi indikator kerusakan hati. Hasil penelitian menunjukkan daging mentah segar keong matah merah (C. obtusa) mempunyai kadar air, protein, lemak, dan abu berturut-turut sebesar 74,22%; 11,99%; 0,63%, dan 8,40%. Ekstrak etanol daging mentah segar keong matah merah memiliki rendemen sebesar 3,41%. Penelitian ini menggunakan 24 ekor tikus putih jantan, yang dibagi secara acak menjadi 4 kelompok dan mendapatkan perlakuan yang berbeda selama 28 hari. Kelompok 1 (kontrol) beri akuades 2 ml/hari, kelompok 2 beri metotreksat 0,125 mg/kg BB/dua hari , kelompok 3 dan 4 diberi ekstrak etanol daging keong matah merah berturut-turut dengan dosis 100 dan 200 mg/kg BB/hari. Pengambilan sampel serum darah dilakukan pada hari ke-29 secara intrakardiak, kemudian dilakukan analisis kadar SGOT dan SGPT. Hasil penelitian menunjukkan bahwa ekstrak etanol daging keong matah merah dengan dosis 100 dan 200 mg/kg BB/hari secara signifikan menurunkan kadar SGOT dan SGPT pada serum tikus putih jantan. KATA KUNCI: ABSTRACT The red eyed snail (Cerithidea obtusa) has been shown to have pharmacological potential as a drug. This study aimed to anal,yze the levels of transaminase enzymes, namely serum glutamic pyruvic transaminase (SGPT) and serum glutamic oxaloacetic transaminase (SGOT) in the serum of male white rats (Rattus novergicus) Sprague Dawley strain given ethanol extract of red eye snail meat (C. obtusa) for 28 days. Levels of SGOT and SGPT which increase simultaneously can be an indicator of liver damage. The results showed that fresh raw meat of red eyed snail (Cerithidea obtusa) had water, protein, fat and ash content respectively of 74.22%; 11.99%; 0.63%, and 8.40%. Ethanol extract of fresh raw meat of red eyed snail has a yield of 3.41%. This study used 24 male white rats which were randomly grouped into 4 groups and received different treatments for 28 days. Group 1 (control) was given a 2 ml of distilled water/day, group 2 was treated with methotrexate 0.125 mg/kg BW/two days, while groups 3 and 4 were fed with ethanol extract of red eye snail meat of 100 and 200 mg/kg BW/day respectively. Blood serum samples were taken by intracardiac method on the 29th day and then analyzed for SGOT and SGPT levels. The results showed that the red eye snail meat ethanol extract with doses of 100 and 200 mg/kg BW/day was significantly able to reduce SGOT and SGPT levels in male white rats serum.

Interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) as proinflammatory cytokines in traumatic brain injury (TBI) of rat (Sprague-Dawley) : A study of propofol administration Riyadh Firdaus; Franciscus Dhyana Giri Suyatna; Aida Rosita Tantri; Yetty Ramli; Ekowati Handharyani; Arni Diana Fitri
Journal of Global Pharma Technology Volume 12 Issue 11 (2020) Nov. 2020
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (756.843 KB)

The increase in the prevalence of traumatic brain injury (TBI) followed by increased morbidity and mortality challenges anesthetists to perform the treatment patients with TBI. Menwhile, interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) are the first pro-inflammatory markers expressed after secondary injury occurs. In addition, propofol (2,6-disopropylphenol) is a short-acting intravenous agent that reported to play role in inflammatory process. This study aims to determine the effects of propofol administration on the neuroinflammatory pathway. Thirty adult female Sprague Dawley rats were randomly assigned as TBI rats (T), rats that received propofol infusion (P), or TBI rats that received propofol infusion (TP). The rat model of TBI was developed using the Marmarou Weight Drop method. Clinical assessment was performed using the Neurobehavioral Severity Score-Revised (NSS-R). Brain tissues were taken 24 h after TBI and the levels of interleukin-1 (IL-1) and interleukin-6 (IL-6) mRNA expression levels were examined using qRT-PCR, while the concentration were examined using ELISA and immunohistochemistry. On clinical examination, a decrease in the median value of NSS-R in the TP group was found from 30th minute to 120th minute. 24 hours after the occurrence of TBI, there was a decrease in both the TP and T groups. However, the NSS-R values in the TP group were lower than those in the T group. The expression of IL-1β was found in the nuclei and cytosol, while IL-6 was found in the axons and cytosol. The expression and concentration of IL-1β and IL-6 were the highest in the TP group, followed by the P and then the T group. Propofol can improve neurological function in rats with TBI, probably through the mechanism involves neuroinflammatory pathways, particularly IL-1β and IL-6.

The Kejadian Pertama Rabbit Haemorrhagic Disease Berdasarkan Studi Seroprevalensi di Provinsi Jawa Barat, Indonesia Retno Setyaningsih; I Wayan Teguh Wibawan; Surachmi Setiyaningsih; Ekowati Handharyani; Sri Murtini; Ahmad Biharidin
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.3.409

Rabbit Haemmorhagic Disease (RHD) is one of viral diseases in rabbits that is still exotic in Indonesia. The RHD disease is caused by the Rabbit Haemorrhagic Disease Virus (RHDV) which is a calicivirus of the genus Lagovirus, Caliciviridae family. The high genetic variation of the RHDV and the rapid spread have the potential to disrupt rabbit farm (rabbittery) production and trade activities, especially rabbit exports, which require clarity on the status and situation of RHD disease in rabbits in Indonesia. A case of suspected RHD in Indonesia was first detected in the Philippines where rabbits exported from Indonesia were detected to be seropositive to RHD which resulted in rabbit export activities to the country was stopped. There is no data on the presence of RHD disease in rabbits in Indonesia, so the seroprevalence study is useful to provide preliminary information on the presence of this disease in Indonesia. This study was conducted on 163 rabbits samples raised in the Lembang area, Bandung, West Java, which is known as the largest rabbit farming center in Indonesia. Rabbit samples were taken at rabbit farms spread across seven villages namely Lembang, Pagerwangi, Cikahuripan, Cikole, Sukajaya, Gudangkahuripan and Jambudipa. The rabbits serum samples obtained were tested using the Indirect Enzym Linked Immunosorbant Assay (ELISA) method to determine the titer of antibodies against RHD. Based on the results of the analysis, it is known that 120 out of 163 rabbit serum samples showed positive antibody titers against RHD. The presence of antibody titers in rabbits on such farms can be preliminary information to be able to carry out further studies.

AKTIVITAS GLIAL FIBRILLARY ACIDIC PROTEIN PADA OTAK MARMUT (Cavia porcellus) SEBAGAI MODEL PENYAKIT ALZHEIMER DENGAN DEPLESI HORMON TESTOSTERON Yuli Purwandari K.; Ekowati Handharyani; Dondin Sajuthi; Erni Sulistiawati
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengarakterisasi marmut sebagai hewan model untuk penyakit Alzheimer dengan mengamati histopatologis otak dan aktivitas seluler glial fibrillary acidic protein (GFAP) pada otak yang diakibatkan oleh deplesi hormon testosteron. Dua belas marmut dibagi dua kelompok berdasarkan umur, yaitu enam marmut umur 16-32 bulan dan enam marmut umur 32-48 bulan. Deplesi testosteron dilakukan dengan cara kastrasi. Dua marmut dari setiap kelompok dinekropsi untuk koleksi sampel otak pada waktu satu, tiga, dan lima bulan setelah kastrasi. Bagian otak yang diambil adalah korteks, lobus parietalis, temporalis, dan hipokampus. Sampel otak dilakukan evaluasi patologis dengan pewarnaan hematoksilin dan eosin dan immunohistokimia dengan antibodi GFAP. Hasil penelitian menunjukkan bahwa deplesi testosteron dapat menyebabkan kerusakan otak yang ditandai oleh kematian sel neuron, peningkatan aktivitas sel-sel glia dan ekspresi GFAP pada jaringan otak.Kesimpulan penelitian adalah penurunan kadar testosteron dalam plasma darah menyebabkan terjadinya kematian sel neuron dan peningkatan aktivitas sel-sel glia pada otak.

DETEKSI IMUNOHISTOKIMIA ANTIGEN Coxiella burnetii SEBAGAI PENYEBAB Q FEVER PADA SAPI Sangko Sayuti Nasution; Agus Setiyono; Ekowati Handharyani
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mendeteksi keberadaan antigen Coxiella burnetii sebagai penyebab Q fever pada organ sapi yang dikumpulkan di rumah potong hewan (RPH) Kota Medan dan Kabupaten Deli Serdang. Pada penelitian ini telah dikumpulkan organ limpa, paru-paru, dan hati dari 162 ekor sapi. Sampel organ tersebut kemudian diperiksa secara imunohistokimia dengan metode streptavidin peroksidase untuk melihat keberadaan antigen Coxiella burnetii menggunakan antibodi poliklonal terhadap Coxiella burnetii. Hasil pemeriksaan imunohistokimia menunjukkan 62/162 (38,3%) sampel sapi imunoreaktif terhadap Coxiella burnetii. Berdasarkan asal pengambilan sampelnya, sebanyak 40/101 (39,6%) sampel sapi yang berasal dari RPH Kota Medan dan 22/61 (36,1%) sampel sapi yang berasal dari RPH di Kabupaten Deli Serdang menunjukkan hasil imunoreaktif. Dilihat dari jenis organnya, dari 162 sampel sapi, antigen Coxiella burnetii dapat dideteksi pada 61 (37,7%) organ limpa, 12 (7,4%) organ paru-paru dan 2 (1,2%) organ hati. Hasil ini menunjukkan telah adanya infeksi Coxiella burnetii pada sapi di Kota Medan dan Kabupaten Deli Serdang.

Analisis Kapasitas Antioksidan dan Sensori Minuman Serbuk Anggur Laut sebagai Terapeutik Obesitas: The Analysis of Antioxidant Capacities and Sensory in Sea Grapes (Caulerpa racemosa) Powdered Drink as a Therapeutic Obesity Dwi Santy Damayati; Evy Damayanthi; Hadi Riyadi; I Wayan Teguh Wibawan; Ekowati Handharyani
Amerta Nutrition Vol. 7 No. 2 (2023): AMERTA NUTRITION (Bilingual Edition)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/amnt.v7i2.2023.175-184

Background: Obesity is a global problem which increasing simultaneously. The high-fat accumulation in the body will result from mild chronic inflammation. Developing a local drink based on seagrapes (Caulerpa racemose) is thought to have a high antioxidant capacity and the potential to become an alternative therapeutic drink for obese people. Seagrapes are easily damaged, so proper drying is needed to maintain antioxidant potential and assisted by adding gum arabic to increase the acceptability. Objectives: To analyze the antioxidant capacity and sensory of sea grapes powder drink (Caulerpa racemose). Methods: The design experiment research was utterly randomized and only used one gum Arabic treatment with a 2.5, 5, and 10% concentration and three replication as sea grapes powder drink samples. The samples were analyzed to seek the antioxidant capacity of DPPH (2,2-diphenyi-1-picrylhydrazyl), CUPRAC (Cupric Ion Reducing Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power). Then research data were analyzed by employing ANOVA and Duncan's follow-up test. Results: The higher concentration of gum Arabic has significantly increased the antioxidant capacity. DPPH, CUPRAC, and FRAP values in 10% gum Arabic concentration were 13.21±0.1 mg/100 g, 25.26±0.5 mg, and 2.89±0.3mg/g. Based on the sensory test results, the panelists preferred the 10% gum Arabic concentration because the color is lighter, can minimize odor and viscosity, and taste better. Conclusions: Seagrapes powder drink with a concentration of 10% gum Arabic has the potential as a therapeutic obesity with the highest antioxidant capacity and is sensory acceptable.

Wound Healing Using White Turmeric (Curcuma zedoaria) Extract Nanoparticles: Macroscopic and Microscopic ObservationWound Healing Using White Turmeric (Curcuma zedoaria) Extract Nanoparticles: Macroscopic and Mi Fitri Ariyani; Lina Noviyanti Sutardi; Ekowati Handharyani
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.441

A wound is damaged of skin tissue caused by physical or mechanical trauma. Naturally, the wound healing process begins almost immediately after the injury occurred and is categorized by four main stage, these are hemostasis, inflammation, proliferation, and maturation. The aim of this research is to observe wound healing process using white turmeric (Curcuma zedoaria) nanoparticles extract gel based on macroscopic and microscopic observations. The skin of 24 Sprague dawley rats were cut 3 cm in the dorsal area and sutured with simple suture technique. The treatments were divided into four groups, positive control (C1), negative control (C2), gel nanoparticles white turmeric extract 0.75% (K1) and 1.5% (K2). Treatment was given every day for a week. Macroscopically observation were made descriptively on the 1st, 3rd, 5th, and 7th days. Histopatologically, the specimens were stained with hematoxylin eosin (HE) to observe the skin structure with parameters scab, epithelial formation, and neovascularization. The observations were evaluated on the 8th days post-injury. Data were presented descriptively through light microscope. The macroscopic results showed the nanoparticles extract gel treatment wound had a faster healing process and lower pain responses than the control groups. The microscopic showed the nanoparticles gel treatment extract had a better repairing process of skin structure and decreased inflammation cells than the control groups. In conclusion, the nanoparticles of white turmeric extract treatment gel group has a better results than the control groups.

Pengaruh aplikasi bovine amniotic membrane pada soket tulang alveolar terhadap ekspresi BMP-2: studi eksperimental in vitro Geneva Ariesta; Octarina Octarina; Elly Munadziroh; Ekowati Handharyani
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 35, No 2 (2023): Agustus 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v35i2.46718

ABSTRAKPendahuluan: Pencabutan gigi dapat menyebabkan resorpsi tulang alveolar. Bovine Amniotic Membrane (BAM) diharapkan menjadi material pengisi soket tulang alveolar sehingga dapat mengurangi terjadinya resorpsi tulang alveolar setelah dilakukan pencabutan. BMP-2 merupakan indikator terjadinya pembentukan tulang alveolar. Tujuan penelitian ini adalah menganalisis ekspresi BMP-2 dengan aplikasi BAM pada soket tulang alveolar tikus Sprague dawley (SD). Metode: …….. …….. Jenis penelitan ini adalah eksperemintal laboratorik. Teknik pengambilan jumlah sampel menggunakan rumus Federer. Sebanyak 16 tikus Sprague dawley jantan dibagi menjadi dua kelompok, yaitu kelompok kontrol dan kelompok BAM. Pencabutan dilakukan pada gigi insisivus sentral kanan mandibula. Setelah dilakukan pencabutan, pada kelompok bam soket alveolar diaplikasikan material BAM dengan ukuran 1,5x10 mm. Hari ke-14 dan ke-28, euthanasia dilakukan, kemudian dilakukan pemotongan pada bagian soket tulang alveolar yang akan dipreparasi menjadi preparat imunohistokimia (IHK). Pembuatan preparat IHK menggunakan pewarnaan antigen-antibodi BMP-2 (1:200, Affinity Biosciences, Inc. USA). Analisis preparat IHK menggunakan mikroskop ZEISS seri AXIO SCOPE A.1 dan software ZEN 3.4 perbesaran 40x. Perhitungan ekspresi BMP-2 menggunakan software imageJ. Analisis data statistik penelitian menggunakan one-way ANOVA dengan uji post hoc Tukey. Hasil: Nilai rerata ekspresi BMP-2 pada kelompok kontrol hari ke-14 dan ke 28 secara berurutan (5,93 ± 0,70) dan (6,60 ± 1,11). Kelompok BAM menunjukkan adanya peningkatan nilai rerata ekspresi BMP-2 pada hari ke-14 dan ke-28 secara berurutan (9,46 ± 0,50) dan (11,73 ± 3,60) secara statistik dengan one way annova pos hoc tukey berbeda bermakna (p<0,05). Simpulan: Aplikasi material BAM pada soket tulang alveolar dapat meningkatkan ekspresi BMP-2 pada hari ke-14 dan 28. Hal tersebut akan menyebabkan penyembuhan tulang alveolar dan berkurangnya resorpsi tulang alveolar.The effect of bovine amniotic membrane application at the alveolar bone socket on the expression of BMP-2: in vitro experimental studyABSTRACTIntroduction: Tooth extraction can cause alveolar bone resorption. Bovine Amniotic Membrane (BAM) is expected to be a filling material for the alveolar bone socket so that it can reduce the occurrence of alveolar bone resorption after extraction. BMP-2 is an indicator of alveolar bone formation. The application of BAM is expected to reduce of alveolar bone resorption after extraction. Aim: The aim of this study was to analyze the expression of BMP-2 by application of BAM in the alveolar bone socket of Sprague Dawley rats (SD). Methods: This type of research is laboratory experimental. The sampling technique used the Federer formula. A total of 16 male SD rats were divided into two groups, which are the control group and the BAM group. Extraction was performed on the mandibular right central incisor. In the BAM group, after removal of the alveolar socket BAM material with a size of 1.5 x 10 mm was applied. On the 14th and 28th day, mice were euthanized and then cut into the alveolar bone socket which will be prepped into an immunohistochemical specimen (IHK). Preparation of IHK will be prepped using BMP-2 antigen-antibody staining (1:200, Affinity Biosciences, Inc. USA). Analysis of CPI preparate using the ZEISS AXIO SCOPE A.1 series microscope and ZEN 3.4 software with 40x magnification. BMP-2 expression calculation using imageJ software. Statistical data analysis of the research used one-way ANOVA with Tukey's post hoc test. Results: The mean value of BMP-2 expression in the control group on day 14 and 28 respectively (5.93 ± 0.70) and (6.60 ± 1.11). The BAM group showed an increase in the mean value of BMP-2 expression on the 14th and 28th days respectively (9.46 ± 0.50) and (11.73 ± 3.60) which were statistically significantly different (p<0, 05). Conclusion: Application of BAM to the alveolar bone socket can increase BMP-2 expression on days 14 and 28. This will lead to alveolar bone healing and reduced alveolar bone resorption.Key wordsbovine amnion membrane, bone morphogenetic protein 2, alveolar socket preservation

AKTIVITAS GLIAL FIBRILLARY ACIDIC PROTEIN PADA OTAK MARMUT (Cavia porcellus) SEBAGAI MODEL PENYAKIT ALZHEIMER DENGAN DEPLESI HORMON TESTOSTERON Yuli Purwandari K.; Ekowati Handharyani; Dondin Sajuthi; Erni Sulistiawati
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2834

Penelitian ini bertujuan mengarakterisasi marmut sebagai hewan model untuk penyakit Alzheimer dengan mengamati histopatologis otak dan aktivitas seluler glial fibrillary acidic protein (GFAP) pada otak yang diakibatkan oleh deplesi hormon testosteron. Dua belas marmut dibagi dua kelompok berdasarkan umur, yaitu enam marmut umur 16-32 bulan dan enam marmut umur 32-48 bulan. Deplesi testosteron dilakukan dengan cara kastrasi. Dua marmut dari setiap kelompok dinekropsi untuk koleksi sampel otak pada waktu satu, tiga, dan lima bulan setelah kastrasi. Bagian otak yang diambil adalah korteks, lobus parietalis, temporalis, dan hipokampus. Sampel otak dilakukan evaluasi patologis dengan pewarnaan hematoksilin dan eosin dan immunohistokimia dengan antibodi GFAP. Hasil penelitian menunjukkan bahwa deplesi testosteron dapat menyebabkan kerusakan otak yang ditandai oleh kematian sel neuron, peningkatan aktivitas sel-sel glia dan ekspresi GFAP pada jaringan otak.Kesimpulan penelitian adalah penurunan kadar testosteron dalam plasma darah menyebabkan terjadinya kematian sel neuron dan peningkatan aktivitas sel-sel glia pada otak.

DETEKSI IMUNOHISTOKIMIA ANTIGEN Coxiella burnetii SEBAGAI PENYEBAB Q FEVER PADA SAPI Sangko Sayuti Nasution; Agus Setiyono; Ekowati Handharyani
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2835

Penelitian ini bertujuan mendeteksi keberadaan antigen Coxiella burnetii sebagai penyebab Q fever pada organ sapi yang dikumpulkan di rumah potong hewan (RPH) Kota Medan dan Kabupaten Deli Serdang. Pada penelitian ini telah dikumpulkan organ limpa, paru-paru, dan hati dari 162 ekor sapi. Sampel organ tersebut kemudian diperiksa secara imunohistokimia dengan metode streptavidin peroksidase untuk melihat keberadaan antigen Coxiella burnetii menggunakan antibodi poliklonal terhadap Coxiella burnetii. Hasil pemeriksaan imunohistokimia menunjukkan 62/162 (38,3%) sampel sapi imunoreaktif terhadap Coxiella burnetii. Berdasarkan asal pengambilan sampelnya, sebanyak 40/101 (39,6%) sampel sapi yang berasal dari RPH Kota Medan dan 22/61 (36,1%) sampel sapi yang berasal dari RPH di Kabupaten Deli Serdang menunjukkan hasil imunoreaktif. Dilihat dari jenis organnya, dari 162 sampel sapi, antigen Coxiella burnetii dapat dideteksi pada 61 (37,7%) organ limpa, 12 (7,4%) organ paru-paru dan 2 (1,2%) organ hati. Hasil ini menunjukkan telah adanya infeksi Coxiella burnetii pada sapi di Kota Medan dan Kabupaten Deli Serdang.

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