Articles
<![CDATA[Parasit Gastrointestinal pada Kadal Naga Berjanggut (Pogona vitticeps) di Cijeruk, Bogor ]]>
<![CDATA[Nisrina Rosyida Noor Rifai]]>;
<![CDATA[Arifin Budiman Nugraha]]>;
<![CDATA[Ekowati Handharyani]]>;
<![CDATA[Fajar Kawitan]]>;
<![CDATA[Umi Cahyaningsih]]>
<![CDATA[ Jurnal Sain Veteriner Vol 41, No 3 (2023): Desember ]]>
Publisher : <![CDATA[Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI ]]>
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DOI: 10.22146/jsv.80391
<![CDATA[Bearded dragons are reptiles that are quite popular as pets. There are many factors of disease that can infect bearded dragons. The environment and feed affect the level of disease threat, such as parasitic infection in the gastrointestinal tract. Data information related to parasitic gastrointestinal infections in bearded dragons in Indonesia is still limited, so supporting examinations are necessary to help diagnose. A total of 38 fecal samples were collected from the bearded dragon in Cijeruk Bogor. Samples were examined by simple flotation method, Ziehl Neelsen staining, and counting the number of parasitic eggs. All samples were positive for gastrointestinal parasite infections such as Choleoeimeria sp., Isospora amphiboluri, Cryptosporidium spp., ascarid egg, and oxyurid egg. The average number of Choleoeimeria sp. and Isospora amphiboluri oocysts were 8730 and 51.281 oocysts per gram of feces. The average number of helminth eggs per gram was 290 and 2325 for ascarid and oxyurid, respectively. Most of the infected bearded dragons do not show significant clinical symptoms. The number of oocysts and worm eggs per gram of feces showed mild and high results.]]>
<![CDATA[Aktivitas Sitotoksik Ekstrak Keong Laut Matah Merah (Cerithidea obtusa) terhadap Sel Kanker Kolon WiDr ]]>
<![CDATA[Wisnu Jaka Dewa]]>;
<![CDATA[Ekowati Handharyani]]>;
<![CDATA[Sri Purwaningsih]]>;
<![CDATA[Silmi Mariya]]>
<![CDATA[ Jurnal Sain Veteriner Vol 42, No 1 (2024): April ]]>
Publisher : <![CDATA[Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI ]]>
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DOI: 10.22146/jsv.83921
<![CDATA[Based on data released by Globocan in 2020, the incidence of colorectal cancer is the fourth highest in Indonesia (8.6%) and the third in the world (10%). This disease is hard to treat because the available therapy is less effective. Therefore, it is necessary to develop effective alternative therapies, especially those originating from Indonesia's natural resources, easy to obtain and reproduce. This study aims to determine the potential of red eye snail extract as an anticancer through cytotoxicity tests with the MTT Assay method on colon cancer cells WiDr and DNA fragmentation tests with Hoescth staining. In this study, we used various concentrations of the red-eye snail extract to test with 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide (MTT) and doxorubicin reagents as positive controls. Absorbance values were read using a microplate reader at a wavelength of 595 nm. The cell absorbance data was converted into cell viability and probit analyzed to obtain the IC50 value. The results showed that the higher the concentration of the extract caused a decrease in the number of WiDr cells and an increase in damage to the structure of WiDr cells. Based on the results of probit analysis, it was found that the IC50 value of the extract was 36.28 µg/mL or classified as moderate cytotoxicity. The DNA fragmentation test showed that at concentrations of 125 ppm and 62.5 ppm, it was able to provide an effect similar to doxorubicin, namely triggering apoptosis in WiDr colon cancer cells.]]>
<![CDATA[Rumput Laut Sango-sango (Gracilaria sp.) Berpotensi Memperbaiki Kondisi Hiperglikemik dan Ekspresi Insulin Pankreas Tikus Diabetik ]]>
<![CDATA[Olga Purnama Bakti]]>;
<![CDATA[Ekowati Handharyani]]>;
<![CDATA[Sri Purwaningsih]]>
<![CDATA[ Jurnal Veteriner Vol 24 No 2 (2023) ]]>
Publisher : <![CDATA[Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association ]]>
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DOI: 10.19087/jveteriner.2023.24.2.179
<![CDATA[Indonesia menempati urutan kelima dengan penderita diabetes melitus terbanyak di dunia. Manajemen makan harus dilakukan terutama pada penderita diabetes melitus yang menggunakan obat penurun glukosa darah atau insulin. Rumput laut sango-sango atau Gracilaria sp., memiliki potensi sebagai agen antidiabetes. Pengujian aktivitas antihiperglikemik pada model hewan diperlukan sebelum implementasi pada manusia. Penelitian dilakukan dengan pemberian pakan dengan penambahan Gracilaria sp. pada tikus dengan periode yang berbeda. Tikus diberi streptozotocin (50 mg/kg bb) untuk menginduksi diabetes. Hewan dibagi dalam enam kelompok perlakuan: kontrol, kontrol diabetes, tikus diabetes diberi pakan standar dan obat metformin, tikus diabetes diberi Gracilaria sp., selama tiga minggu dan dua minggu, dan tikus normal diberi Gracilaria sp., selama empat minggu. Parameter yang digunakan adalah glukosa darah dan luas area positif ekspresi insulin. Hasil yang didapatkan adalah pemberian streptozotocin mengakibatkan keadaan diabetes pada tikus. Pakan dengan penambahan Gracilaria sp., menunjukkan potensi untuk membantu manajemen hiperglikemik. Hasil yang signifikan ditemukan pada area positif ekspresi insulin. Pemberian pakan dengan penambahan Gracilaria sp., dengan hasil terbaik diperoleh pada pakan dengan pemberian selama tiga minggu.]]>
<![CDATA[Laporan Kasus: Studi Morfopatologi Demam Babi Afrika pada Babi di Bogor, Jawa Barat ]]>
<![CDATA[Rahayu Woro Wiranti]]>;
<![CDATA[Dwi Utari Rahmiati]]>;
<![CDATA[Aldo Yanuar Wuriyantara]]>;
<![CDATA[Okti Nadia Poetri]]>;
<![CDATA[Ekowati Handharyani]]>
<![CDATA[ Jurnal Veteriner Vol 24 No 4 (2023) ]]>
Publisher : <![CDATA[Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association ]]>
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DOI: 10.19087/jveteriner.2023.24.4.564
<![CDATA[Demam Babi Afrika atau African swine fever (ASF) adalah penyakit menular yang dapat terjadi pada ternak babi dan babi liar. Penyakit tersebut disebabkan oleh virus ASF. Gejala klinis sangat bervariasi dari kasus yang terjadi perakut, akut, subakut dan kronis tergantung pada virulensi virus. Kejadian ASF dapat menyebabkan angka kematian tinggi pada babi, penurunan kondisi sosial, dan ekonomi termasuk kontaminasi lingkungan. Berdasar beberapa pertimbangan masalah tersebut, maka akan sangat bermakna bila dilakukan studi morfopatologi dan klarifikasi terhadap kejadian ASF pada babi (Sus scrofa domesticus). Kajian saat ini dilaksanakan dengan melakukan prosedur nekropsi terhadap dua ekor babi umur empat bulan, yaitu dengan melakukan pemeriksaan kondisi tubuh dan organ-organ interna. Sampel organ diambil untuk pemeriksaan histopatologi yang selanjutnya diwarnai dengan hematoksilin-eosin (HE). Pemeriksaan lanjut dilakukan dengan metode polymerase chain reaction (PCR) menggunakan primer universal (p72 dan p54). Hasil pemeriksaan makroskopik menunjukkan bahwa beberapa bagian kulit mengalami erythema, terjadi splenitis hemoragi, enteritis hemoragi disertai dengan lymphadenopathy pada limfonodus mesenterika dan terjadi hemoragi pada meningen dan pembuluh darah di otak. Hasil pemeriksaan histopatologimenunjukkan lesi pada organ limforetikuler seperti limpa dan limfonodus mesenterika, yaitu radang limfositik disertai hemoragi. Pemeriksaan pada otak menunjukkan lesi radang limfositik disertai hemoragi pada selaput meningen. Hasil pemeriksaan molekuler dengan metode PCR memberikan konfirmasi bahwa penyakit disebabkan oleh virus ASF. Berdasarkan hasil pemeriksaan komprehensif secara makroskopik, mikroskopik dan evaluasi PCR dapat disimpulkan bahwa kedua ekor babi tersebut menderita infeksi ASF tipe akut.]]>
<![CDATA[Resistansi Escherichia coli patogen asal ayam petelur terhadap beberapa antibiotik ]]>
<![CDATA[Suhendi, Adnan Rizal]]>;
<![CDATA[Afiff, Usamah]]>;
<![CDATA[Handharyani, Ekowati]]>
<![CDATA[ Current Biomedicine Vol. 2 No. 1 (2024): January ]]>
Publisher : <![CDATA[School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia ]]>
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DOI: 10.29244/currbiomed.2.1.45-54
<![CDATA[Latar Belakang: Penggunaan antibiotik yang tidak tepat pada ternak menyebabkan munculnya resistansi bakteri terhadap antibiotik. Salah satu bakteri yang telah dilaporkan resistan adalah Escherichia coli sehingga pengobatan untuk infeksi bakteri ini menjadi kurang efektif. Tujuan: Penelitian ini bertujuan untuk mendeskripsikan resistansi E. coli patogen yang berasal dari usap kloaka ayam petelur terhadap antibiotik. Metode: Sebanyak 20 sampel usap kloaka diidentifikasi keberadaan E. coli melalui pengamatan makroskopis, mikroskopis, uji biokimia, dan fermentasi karbohidrat uji patogenisitas dilakukan dengan menggunakan media Congo red. Isolat yang patogen selanjutnya diuji kepekaannya terhadap 6 jenis antibiotik dengan uji kepekaan difusi cakram Kirby-Bauer. Hasil: Penelitian memperoleh 15 isolat E. coli dan uji Congo red mendapatkan 4 isolat E. coli patogen. Hasil penelitian menunjukkan bahwa 75% isolat resistan terhadap azitromisin, 100% isolat resistan intermediet terhadap doksisiklin dan siprofloksasin. Sementara itu, 75% isolat masih sensitif terhadap gentamisin dan 100% isolat sensitif terhadap kloramfenikol dan amoksisilin. Kesimpulan: Penelitian ini berhasil mengisolasi E. coli yang bersifat patogen dari ayam petelur yang telah resistan terhadap beberapa antibiotik yang diujikan.]]>
<![CDATA[Aktivitas Gastroprotektif Infusa Serai Wangi terhadap Gastritis Akut pada Tikus Sprague dawley ]]>
<![CDATA[Nabilah, Avida Shahnaz]]>;
<![CDATA[Handharyani, Ekowati]]>;
<![CDATA[Sutardi, Lina Noviyanti]]>;
<![CDATA[Mustika, Aulia Andi]]>
<![CDATA[ Acta VETERINARIA Indonesiana Vol. 12 No. 1 (2024): Maret 2024 ]]>
Publisher : <![CDATA[IPB University ]]>
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DOI: 10.29244/avi.12.1.17-24
<![CDATA[Gastritis is an inflammation of the stomach mucosal lining. Citronella grass (Cymbopogon nardus) is a plant that has been used in traditional medicine to treat diseases, including digestive problems. This study aimed to analyze the gastroprotective activity of citronella grass infusion on acidified ethanol-induced gastritis in Sprague Dawley rats. The study was conducted on four groups of rats that were previously fasted for 18 hours. All rats were given treatments: water (HCl/ethanol group), omeprazole (omeprazole group), 20% citronella grass infusion (P1), and 40% citronella grass infusion (P2). After 90 minutes, all groups were administered HCl and ethanol mixture orally and then sacrificed 90 minutes later. The stomachs were collected for gastric fluid pH measurement and macroscopic and microscopic analysis. The results of phytochemical screenings showed that the citronella grass infusion contains alkaloids, flavonoids, saponins, and tannins. The pH values and the gastric lesion scores of P1 and P2 were significantly different (P<0.05) with higher and lower values respectively than the HCl/ethanol group. Macroscopic and microscopic examinations also showed a decrease in gastric lesions in the P1 and P2 groups. These findings confirm the gastroprotective activity of citronella plant infusion. There was no significant difference (P>0.05) between the P1 and P2.]]>
<![CDATA[Kasus Rabbit Haemorrhagic Disease (RHD) pada Kelinci: Sejarah, Penyebaran, serta Dampak RHDV di Beberapa Negara Asal Kelinci Impor Indonesia ]]>
<![CDATA[Setyaningsih, Retno]]>;
<![CDATA[Murtini, Sri]]>;
<![CDATA[Wibawan, I Wayan Teguh]]>;
<![CDATA[Setiyaningsih, Surachmi]]>;
<![CDATA[Handharyani, Ekowati]]>
<![CDATA[ Acta VETERINARIA Indonesiana Vol. 12 No. 1 (2024): Maret 2024 ]]>
Publisher : <![CDATA[IPB University ]]>
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DOI: 10.29244/avi.12.1.8-16
<![CDATA[Virus Rabbit Haemorrhagic Disease (RHDV) adalah virus penyebab Rabbit Haemorrhagic Disease (RHD) pada kelinci Eropa (Oryctolagus cuniculus). Penyakit ini memiliki tingkat kematian dan morbiditas hingga 100%. Penyakit ini pertama kali dilaporkan di Republik Rakyat Tiongkok pada tahun 1984 dan menyebar ke seluruh dunia. Pada tahun 1996, kasus pertama infeksi RHDV pada kelinci tanpa gejala klinis menyebar di peternakan kelinci di Italia. Beberapa studi telah melapaorkan keberadaan variasi genetic RHDV termasuk varian RHDV terbaru. Penyakit ini berpotensi menimbulkan dampak ekologis dan kerugian ekonomi, terutama di peternakan kelinci. Pada studi sebelumnya diketahui bahwa terdapat serokonversi RHDV pada kelinci tanpa gejala klinis di daerah Bandung Barat, Provinsi Jawa barat, Indonesia, meskipun belum ada informasi mengenai keberadaan RHDV di Indonesia. Meskipun Indonesia diketahui berstatus bebas RHDV, namun kelinci yang diimpor berasal dari negara-negara di Eropa, Amerika, dan Australia yang terkenal sebagai negara endemik RHDV. Tulisan ini bertujuan untuk mengkaji penyebaran RHDV di negara asal kelinci impor Indonesia dan dampaknya. Selain itu, tulisan ini diharapkan dapat memberikan informasi tentang penyakit kelinci yang sedang berkembang.]]>
<![CDATA[Evaluation of Ashitaba (Angelica keiskei) Crackers Formulations as α-Glucosidase Enzyme Inhibitors ]]>
<![CDATA[Khoiriyah, Romyun Alvy]]>;
<![CDATA[Marliyati, Sri Anna]]>;
<![CDATA[Ekayanti, Ikeu]]>;
<![CDATA[Handharyani, Ekowati]]>
<![CDATA[ Media Gizi Indonesia Vol. 19 No. 2 (2024): MEDIA GIZI INDONESIA (NATIONAL NUTRITION JOURNAL) ]]>
Publisher : <![CDATA[Universitas Airlangga ]]>
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DOI: 10.20473/mgi.v19i2.198-205
<![CDATA[One of the global health issues is diabetes mellitus, characterized by elevated blood glucose levels. The absorption of glucose in the body occurs through the digestion of carbohydrates by the enzyme α-glucosidase, which is responsible for hydrolyzing carbohydrates into sugar. Ashitaba (Angelica keiskei) is a herbal plant from Japan and has long been utilized in traditional medicine for its various health benefits and preventive properties against multiple diseases. This plant has also been cultivated in Indonesia, including Trawas Mojokerto, East Java. This study aims to develop ashitaba crackers and evaluate their potential α-glucosidase inhibitory activity to develop safe and effective natural products to assist in managing diabetes more efficiently. This research was conducted from July to September 2022. Two formulations of fresh ashitaba leaves were used (12% CAST 1 and 18% CAST 2 of 100 g of flour). Based on the results of this research, chemical composition tests showed that the carbohydrate and dietary fiber content in CAST 1 were higher than those in CAST 2. Protein, lipid, water, and ash content in CAST 2 had higher values than in CAST 1. Flavonoid content and inhibitory activity test results of ashitaba crackers showed significant differences between the two formulation groups regarding flavonoid content and percentage inhibition against the α-glucosidase enzyme. Both flavonoid content and inhibitory activity in CAST 2 were higher than in CAST 1. Ashitaba crackers with the best formulation in this study, based on flavonoid levels and their ability to inhibit α-glucosidase enzyme, were identified as CAST 2.]]>
<![CDATA[Aktivitas Anticestoda In Vitro Metabolit Sekunder Daun Miana (Coleus blumei. Benth) terhadap Cacing Hymenolepis microstoma ]]>
<![CDATA[Ridwan, Yusuf]]>;
<![CDATA[Satrija, Fadjar]]>;
<![CDATA[Handharyani, Ekowati]]>
<![CDATA[ Jurnal Medik Veteriner Vol. 3 No. 1 (2020): April ]]>
Publisher : <![CDATA[Universitas Airlangga ]]>
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DOI: 10.20473/jmv.vol3.iss1.2020.31-37
<![CDATA[Penelitian ini untuk mengetahui golongan senyawa metabolit sekunder dari ekstrak daun miana (Coleus blumei. Benth) yang memiliki aktivitas anticestoda. Daun miana kering diekstraksi dengan metode yang sesuai untuk mendapatkan golongan senyawa metabolit sekunder alkaloid, flavonoid, tanin, dan steroid. Aktivitas anticestoda diukur dengan menghitung waktu kematian cacing Hymenolepis microstoma pada setiap golongan metabolit sekunder konsentrasi 10%. Hasil penelitian menunjukkan tanin memiliki aktivitas anticestoda yang kuat yang tidak berbeda dengan praziquatel sebagai anthelmintik komersial. Akivitas anticestoda flavonoid mirip dengan tanin akan tetapi aktivitasnya masih dibawah praziquatel. Alkaloid dan steroid memiliki aktivitas anticestoda yang lebih rendah dibandingkan dengan tanin dan flavonoid. Hasil penelitian ini membuktikan bahwa tanin sebagai senyawa metabolit sekunder yang paling aktif sebagai anticestoda dibandingkan flavonoid, alkaloid, dan steroid.]]>
<![CDATA[The effect of application of bovine amniotic membrane on osteoblasts, osteocytes, and collagen in the post-extraction alveolar bone socket of Sprague Dawley rats ]]>
<![CDATA[Wibowo, Artdhea Regita]]>;
<![CDATA[Octarina, Octarina]]>;
<![CDATA[Munadziroh, Elly]]>;
<![CDATA[Handharyani, Ekowati]]>
<![CDATA[ Padjadjaran Journal of Dentistry Vol 35, No 2 (2023): July 2023 ]]>
Publisher : <![CDATA[Faculty of Dentistry Universitas Padjadjaran ]]>
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DOI: 10.24198/pjd.vol35no2.46522
<![CDATA[ABSTRACTIntroduction: Tooth extraction can result in loss of alveolar bone. The application of biomaterials Bovine Amniotic Membrane (BAM) that containing growth factors and collagen is expected to play a role in socket preservation. This study aimed to observe the effect of BAM application on the growth of osteoblasts, osteocytes, and collagen in histological preparate of the alveolar bone socket of Sprague Dawley rats (SD). Methods: 16 male SD rats were used in this group. SD rats were extracted on the mandible central incisivus. Afterward, it was divided into two groups, specifically the control group (without treatment) and the treatment group (BAM application). After extraction, the treatment group was applied with BAM with a size of 1.5 mm x 10 mm in socket alveolar bone. On the 14th and 28th day, the rats were euthanized and the alveolar bone socket of mandibular tissue was taken and stained with Hematoxylin Eosin (HE) and Masson Trichrome (MT). Results: The results of all groups showed that the number of osteoblasts and collagen thickness was higher on day 14th compared to day 28th, while osteocytes continued to increase from day 14th to 28th. The thickness of collagen, osteoblasts, and osteocytes in groups with BAM application was higher than without application. Statistical results showed that there were significant differences between groups (p<0.05). Conclusion: The application of BAM can act as material preservation socket. This biomaterial can accelerate recovery by increasing collagen thickness, osteoblasts, and osteocytes. ]]>
