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All Journal International Journal of Renewable Energy Development Journal on Mathematics Education (JME) GRADIEN Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Masharif al-Syariah: Jurnal Ekonomi dan Perbankan Syariah J-EBIS (Jurnal Ekonomi dan Bisnis Islam) TUNAS BANGSA JURNAL SERAMBI ILMU TAZKIR: Jurnal Penelitian Ilmu-ilmu Sosial dan Keislaman JURNAL PENDIDIKAN TAMBUSAI Jurnal Basicedu Jurnal Dedikasi Pendidikan At-tijaroh: Jurnal Ilmu Manajemen dan Bisnis Islam EDUTEC : Journal of Education And Technology Journal of halal product and research (JHPR) International Journal of Marketing and Human Resource Research Munaddhomah: Jurnal Manajemen Pendidikan Islam Jurnal Bisnis Mahasiswa Journal Of Sharia Banking INSANIA : Jurnal Pemikiran Alternatif Kependidikan Jurnal Basicedu JIFTECH: Journal Of Islamic Financial Technology Jurnal Kedokteran Hewan Nanggroe: Journal Of Scholarly Service Nanggroe: Journal of Scholarly Service JSIKOM (Jurnal Studi Ilmu Komunikasi) Krigan: Journal of Management and Sharia Business Jurnal Mediasas : Media Ilmu Syari'ah dan Ahwal Al-Syakhsiyyah Jurnal Medika Veterinaria At Tahkim Jurnal Hukum Ekonomi Syariah Profetik Jurnal Ekonomi Syariah Jurnal Pendidikan MIPA Yuriska : Jurnal Ilmiah Hukum Singkite Journal Jurnal Ilmiah Mahasiswa Pendidikan Geografi ILLAT-J
Muhammad Isa
Universitas Sumatera Utara
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Journal : Jurnal Kedokteran Hewan

 1 
THE EFFECT OF SUPEROVULATION INDUCTION USING FSH ON INCREASING FSH AND LH CONCENTRATIONS IN NEW ZEALAND WHITE RABBITS Wahyuni, Sri; Fathurrahman, Fadhil; Adam, Mulyadi; Syafruddin, Syafruddin; Roslizawaty, Roslizawaty; Hasan, Muhammad; Melia, Juli; Dasrul, Dasrul; Isa, Muhammad; Rosmaidar, Rosmaidar
Jurnal Kedokteran Hewan Vol 18, No 1 (2024): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i1.37513

Abstract

This study aimed to determine the concentrations of Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) in New Zealand White (NZW) rabbits after superovulation induction using FSH. Six female NZW rabbits and one male NZW rabbit were used in this study. Criteria for female NZW rabbits used include having previously given birth, not being pregnant, and having a body weight of 2-3 kg. The rabbits were divided into two treatment groups; the first group was injected with physiological NaCl (NZW1, n= 3), while the second group was injected with FSH (NZW2, n= 3). Injections of physiological NaCl or FSH were administered five times at 12-hour intervals and first injection was given at 20:00 p.m. In NZW1, each injection was given physiological NaCl with volumes of 0.2:0.2+0.2;0.4+0.4 mL, respectively whereas in NZW2, each injection was given FSH with doses of 4:4+4:8+8 mg, respectively. Twelve hours after the last FSH injection, NZW2 rabbits were injected with 100 IU hCG and mated with male rabbit, while NZW1 rabbits were mated without hCG administration. On days 1, 3, and 5 after mating, blood samples were collected for FSH and LH hormone examination using the Enzyme-Linked Immunosorbent Assay (ELISA) method. The FSH concentrations on days 1, 3, and 5 between NZW1 vs NZW2 were 34.781.46 vs 39.753.18; 41.434.90 vs 41.425.14; and 44.471.51 vs 30.130.19 mIU/mL, respectively (P0.05). The LH concentrations on days 1, 3, and 5 between NZW1 vs NZW2 were 33.784.01 vs 40.1812.36; 29.342.24 vs 31.3110.13; and 42.0911.38 vs 30.578.87 mIU/mL, respectively (P0.05). It is concluded that FSH administration does not affect the concentrations of FSH and LH in NZW rabbits. The FSH concentration five days after mating tends to fluctuate, while the LH concentration tends to decrease as the days increased.
THE EFFECT OF AEROB AND ANAEROBIC CONDITION AND ADDITION OF CaCO3 ON THE GROWTH AND ANTIBACTERIAL ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM LAYER HENS INTESTINE AFTER ADMINISTRATED OF AKBISprob Nurliana, Nurliana; Khairunisa, Fauzia; Musni, Nailah; Vanda, Henni; Rasmaidar, Rasmaidar; Hasan, Muhammad; Asmilia, Nuzul; Sari, Wahyu Eka; Isa, Muhammad
Jurnal Kedokteran Hewan Vol 17, No 3 (2023): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i3.20309

Abstract

This study aimed to determine the effect of aerobic and anaerobic incubation, and the addition of CaCO on the growth and antibacterial activity of Lactic Acid Bacteria (LAB) isolated from laying hens intestines (strain ISA Brown) after given 4% AKBISprob. The LAB used in this study was the isolates strains that produced the strongest antibacterial activity with inhibition zone 5 mm. The treatments consisted of two incubations condition (aerobic and anaerobic) with five concentrations of CaCO3 (1%, 2%, 3%, 4%, and 5%). Each treatment consisted of three repetitions. Total Plate Count (TPC) method and the agar diffusion method were used to calculate the amount of LAB and antibacterial activity, respectively. The results showed that anaerobic conditions had a significant effect (P0.05) on the increase of LAB growth, but both of aerobic and anaerobic conditions (P0.05) did not show significant effect on antibacterial activity. The addition of CaCO3 had a significant effect (P0.05) on the increase in growth and antibacterial activity of LAB. Based on the results of the study, it can be concluded that anaerobic incubation can increase the growth of two isolate of LAB isolates (P31S72 and P31H62), however aerobic and anaerobic incubation have no effect on antibacterial activity. In addition, the administration of 3% CaCO3 can increase the growth and antibacterial activity of all LAB isolates.
ISOLASI DAN IDENTIFIKASI VIRUS AVIAN INFLUENZA PADA BERBAGAI SPESIES UNGGAS SECARA SEROLOGIS DAN MOLEKULER (Isolation and Identification of Avian Influenza in Different Species of Poultry by Means of Serological and Molecular Methods) Helmi, Teuku Zahrial; Tabbu, Charles Rangga; Artama, Wayan Tunas; Haryanto, Aris; Isa, Muhammad
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3378

Abstract

The purpose of this research was to identify avian influenza (AI) virus using serological and molecular methods on poultry which suspected as AI infected in Aceh province. This study used 37 samples of tracheal and cloacal swabs and organs from various species of poultry that were collected from several districts/cities in Aceh. Samples were collected and put into transport media and stored at 4 C before sending to the laboratory. Samples were inoculated in specific pathogen-free of embryonated chicken egg with the age of 9-11 days for further serological and molecular examination. From 37 samples which infected to embryonated chicken egg then followed by hemagglutinin agglutination test/hemagglutinin inhibition revealed that 7 samples were positively infected with AI virus. The amplification result of specific matrix gene primer was followed by electrophoresis on 2% agarose gel which were obtained in the form of a deoxyribonucleic acid (DNA) band at 276 bp for matrix gene and 1.725 bp for H5 gene for all isolates test. In conclusion, the virus which caused the death of various types of poultry in Aceh province is avian influenza A virus subtype H5.Key words: avian influenza virus, H5N1, serologic, matrix, heamaglutinin
RESPONS HSP-70 DAN KADAR KORTISOL AKIBAT PEMBERIAN KOMBINASI EKSTRAK JALOH DAN KROMIUM PADA AYAM BROILER YANG MENGALAMI CEKAMAN PANAS s, Sugito; Rahmi, Erdiansyah; Isa, Muhammad
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.341

Abstract

Penelitian ini bertujuan mengetahui pengaruh pemberian kombinasi ekstrak jaloh dan kromium terhadap pembentukan heat shock protein-70 (HSP-70) dan kadar kortisol ayam yang mengalami cekaman panas. Dalam penelitian ini digunakan ayam broiler sebanyak 12 ekor yang dibagi atas empat perlakuan: Perlakuan I (EC), cekaman panas + dan kombinasi 1.000 mg ekstrak jaloh dengan 1.000 g kromium per liter air minum, perlakuan II (E), cekaman panas + 1.000 mg ekstrak jaloh per liter air minum, perlakuan III (KD), cekaman panas + 0,0 mg ekstrak jaloh dan 0,0 g kromium, dan Perlakuan IV (KL), tanpa cekaman panas dan 0,0 mg ekstrak jaloh dan 0,0 g kromium. Masing-masing perlakuan terdiri atas tiga ulangan. Metode pemberian cekaman panas dilakukan dengan meningkatkan suhu dalam kandang pada 331 C selama lima jam per hari dalam waktu lima belas hari. Pemberian kombinasi ekstrak jaloh dan kromium dilakukan dengan cara melarutkannya dalam air minum dan diberikan selama dua jam (pemberian pukul 10.00) sebelum suhu di dalam kandang mencapai 331 C. Pengambilan sampel serum dan jaringan paru dilakukan pada hari ke-15 pelaksanaan penelitian (ayam umur 36 hari). Pengambilan sampel darah dilakukan sebelum ayam dipotong dan jaringan organ paru diambil setelah ayam dipotong. Pada sampel serum dilakukan pemeriksaan kortisol dengan enzymelinkedimmunosorbantassay (ELISA) dan deteksi HSP-70 di dalam jaringan paru dilakukan menggunakan metode imunohistokimia. Hasil penelitian ini menunjukkan bahwa pemberian cekaman panas pada suhu 331 C selama lima jam per hari dapat meningkatkan pembentukan HSP-70 dalam paru dan kadar kortisol dalam serum. Pemberian ekstrak jaloh secara tunggal lebih efektif menurunkan jumlah HSP-70 pada jaringan paru dibandingkan jika dikombinasi dengan kromium.
Co-Authors Abir, Ismail Ahmad Adjeng Moelyati, Trisniarty Adzkia, Nida Agasta, Rio Ahmasi, Latif Al Aji, Mochamat Bayu Ali Hardana Almegi, Almegi Aris Haryanto Asnil Aidah Ritonga Athaillah, Farida Azhar Azhar Azwar Hamid Bayurini Samudra, Sari Cesarian, Dwiky Pobri Charles Rangga Tabbu Daniel Daniel Dasrul Dasrul Deni Kurniawan, Deni Didik Sugiyanto Efendi, Ilham Eka Safitri Elin Yusibani Erdiansyah Rahmi Fachruddin Azmi, Fachruddin Fadlilah, Hamni Fathurrahman, Fadhil Frengki Frengki, Frengki Ginting, Budi Setiawan Hambali Hambali Harahap, Desi Anora Heni Pujiastuti Henni Vanda Hutagaol, Abdul Latif Hutasuhut, Sartika Irwansya, Muhammd Juli Melia Khairunisa, Fauzia Khurniawan, Deni Lestari, Emmi Lubis, Aswadi Lubis, Zulfahmi M. Yusuf Mesiono, Mesiono Mudofir, Imam Muhammad Hasan Muhammad Yusuf Ibrahim Mulyadi Adam, Mulyadi Murni Murni Musni, Nailah Mutia, Fara Nasution, Adanan Murroh Neliwati Nofinawati Nofinawati Nofinawati, Nofinawati Noviabahari, Jannatul Laily NURLIANA NURLIANA Nurul fadillah, Nurul Nuzul Asmilia Palupi, Dian Prasetyo Yekti Utomo Priatna, Rusdiana Putra, Septian Dwi Maulana Rahil Helmi Rahmadi Rahmadi Rasmaidar Rasmaidar Reza Aprianti, Reza Riski, Novia Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar, Rosmaidar Rully Charitas Indra Prahmana Saleh, Idris Sari, Wahyu Eka Saudah Setyawan, Dimas Ari Simahatie, Mai SIREGAR, BUDI GAUTAMA Siti Aisyah Siti Khadijah Siti Sarah, Siti SRI RAHAYU Sri Wahyuni Suci Maulina Sugito Sugito Sukmawati, Nur Sulaeman Sulaeman Surbakti, Muhammad Syukri Sutantohadi, Alief Suyatmika, Yulita Syafruddin Syafruddin Tauvani, A Yuli Teuku Zahrial Helmi Umar, Muksin Utari, Lin Putri Vahreza, Anshar Wardah Manurung, Sri Rezeqi Rafiqah Wayan Tunas Artama Widodo, Yulius Harry Willy Syarif Yandy, Eza Tri Yani, Nuriza Yundari, Yundari Yusuf Hadijaya Zahratun Zaini, Nasrullah