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Sifat Fisik dan Kecernaan Ransum Sapi Bali yang Mengandung Hijauan Beragam N. N. Suryani; I. G. Mahardika; S. Putra; N. Sujaya
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 17, No 1 (2015): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.17.1.38-45.2015

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh komposisi dan jenis hijauan beragam terhadap sifat fisik dan kecernaan ransum pada sapi Bali. Percobaan menggunakan Rancangan Acak Kelompok (RAK) terdiri dari 4 perlakuan ransum dengan 3 kelompok berat badan sebagai ulangan. Perlakuan ransum disusun berdasarkan bahan kering adalah: (A) rumput gajah 45% +  jerami padi 0%  +  gamal 15% + kaliandra 10% + konsentrat 30%; (B) rumput gajah 30% +  jerami padi 10% +  gamal 20% + kaliandra 10% + konsentrat 30%; (C) rumput gajah 15% + jerami padi 20% + gamal 25% + kaliandra 10% + konsentrat 30% dan (D) rumput gajah 0% + jerami padi 30% + gamal 30% + kaliandra 10% +  konsentrat 30%. Peubah yang diukur adalah sifat fisik dan kecernaan ransum. Data dianalisis menggunakan sidik ragam. Hasil penelitian menunjukkan, sapi Bali yang diberi perlakuan D memiliki densitas tertinggi (P<0,05) yaitu 0,313 g/ml, KCBK dan KCPK tertinggi (P<0,05) masing-masing 67,78% dan 71,42% namun KCSK terendah (P<0,05) yaitu 49,34%. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa ransum D mampu meningkatkan densitas dan daya serap air serta meningkatkan KCBK dan KCPK ransum.
Penentuan Keseimbangan Protein dan Energi Ransum Sapi Bali Jantan N. P. Mariani; I. G. Mahardika; S. Putra; I. B. G. Partama
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 17, No 1 (2015): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.17.1.46-53.2015

Abstract

Penelitian ini bertujuan untuk mengetahui keseimbangan energi dan protein sapi Bali jantan yang diberi ransum dengan level protein dan energi.  Rancangan yang digunakan : rancangan acak kelompok (RAK) dengan lima perlakuan dan tiga kelompok bobot badan. Bobot sapi berkisar    198,67 – 207,00 kg. Kelima perlakuan ransum yaitu: (A) 15,42% protein dan GE 4,02 Mkal/kg DM; (B) 14,74% protein dan GE 3,75 Mkal/kg DM; (C) 13,11% protein dan GE 3,79 Mkal/kg DM; (D) 10,33% protein dan GE 3,92 Mkal/kg DM dan (E) 10,58% protein dan GE 3,53 Mkal/kg DM. Peubah yang diukur : konsumsi energi, konsumsi protein, energi tercerna, protein tercerna, energi termetabolis, retensi energi dan retensi protein. Hasil penelitian menunjukkan konsumsi energi, energi tercerna dan energi termetabolis tidak menunjukkan perbedaan yang  nyata (P>0,05), sedangkan  konsumsi protein, protein tercerna, retensi energi dan retensi protein pada perlakuan A nyata lebih tinggi (P<0,05) dibandingkan dengan perlakuan E. Hasil penelitian ini dapat disimpulkan  konsumsi energi, konsumsi protein, energi tercerna, protein tercerna, retensi energi dan retensi protein tertinggi pada imbangan 15,42% protein dan GE 4,02 Mkal/kg DM.
Energy and Nitrogen Retention of Bali Heifers (Bos sondaicus) Fed Diet Containing Different Energy Protein Level Ni Nyoman Suryani; I Wayan Suarna; I Gede Mahardika; Ni Putu Sarini; Lindawati Doloksaribu
TERNAK TROPIKA Journal of Tropical Animal Production Vol 21, No 1 (2020): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2020.021.01.9

Abstract

Database of Bali heifer particularly on their nutrient digestibility, energy and nitrogen balance of various ration formulas on their growth performance were limited.  A randomized block design with four types rations of metabolizable energy (ME) and crude protein ratios, i.e. of 2,045.38 kcal ME/kg:12.06% (Treatment A), 2,103.57 kcal ME/kg:13.11% (Treatment B), 2,201.85 kcal ME/kg:13.97% (Treatment C) and 2297.60 kcal ME/kg:15.05% (Treatment D) were conducted to evaluate nutrient digestibility, energy and nitrogen retention of Bali heifers.  The rations consisted of concentrate, urea, molasses, king grass, coconut oil and vitamin-mineral mix.  Results showed that Treatment D improved significantly of the 7,814.34 kcal/d digestible energy, 49.87 g/d digestible nitrogen, 11,015.06 kcal/d energy intake, 423.53 g/d nitrogen intake, 67.76 kcal/d energy retention, 7.91 g/d nitrogen retention, 0.33 kg/d daily weight gain (ADG), (P<0.05).  In summary, ration for Bali heifer should contain at least 2,297.60 kcal ME/kg:15.05% crude protein for better average daily weight gain.
TOKSISITAS ISOLAT DARI EKSTRAK METANOL SPONS Clathria (Thalysias) sp TERHADAP LARVA Artemia salina L. Putu Lakustini Cahyaningrum; I Made Dira Swantara; I Gede Mahardika
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 3 No 2 (2015)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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Abstract

ABSTRAK : Telah dilakukan uji toksisitas dari ekstrak metanol spons Clathria (Thalysias) sp dengan metode Brine Shrimp Lethality Test (BSLT) menggunakan larva Artemia salina L. Ekstrak metanol spons Clathria (Thalysias) sp dipartisi dengan pelarut n-heksana, kloroform dan air. Partisi dari 19,31 gram ekstrak metanol menghasilkan ekstrak n-heksan sebanyak 1,93 gram, ekstrak kloroform sebanyak 2,48 gram, dan ekstrak air sebanyak 12,17 gram. Hasil uji toksisitas menunjukkan ekstrak kloroform memiliki toksisitas paling tinggi dengan LC50 64,57 ppm. Selanjutnya Ekstrak kloroform dipisahkan dengan kromatografi kolom silika gel menggunakan eluen n-heksana : etil asetat (8:2), diperoleh 4 fraksi yaitu  FA, FB, FC, dan FD. Fraksi B (FB) memberikan nilai toksisitas paling tinggi dengan nilai LC50 72,44 ppm. Identifikasi isolat FB dilakukan dengan uji fitokimia yang menunjukkan adanya senyawa steroid.ABSTRACT : Toxicity tests have been conducted of the methanol extract of the sponge Clathria (Thalysias) sp with methods Brine Shrimp Lethality Test (BSLT) using larvae of Artemia salina L. Methanol extract of the sponge Clathria (Thalysias) sp was partitioned with n-hexane, chloroform and water. Partitioning of 19.31 grams of methanol extract with n-hexane yielded as much as 1.93 grams, 2.48 grams of total chloroform extract, and as much as 12.17 grams of water extract. The toxicity test showed that chloroform extract had the highest toxicity with LC50 of 64.57 ppm. Furthermore chloroform extract was separated by silica gel column chromatography using n-hexane eluent: ethyl acetate (8: 2), obtained 4 fractions which were FA, FB, FC, and FD. Fraction B (FB) provides the highest value of toxicity LC50 value of 72.44 ppm. Identification of isolates FB conducted by phytochemical test that indicates that the presence of steroid compounds. 
PENGARUH MINYAK JELANTAH DAN WAKTU INKUBASI TERHADAP AKTIVITAS LIPASE PADA TANAH HUTAN MANGROVE PANTAI TABLOLONG KUPANG Gusti A Malelak; I Nengah Wirajana; I Gede Mahardika
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 2 No 2 (2014)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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Abstract

ABSTRAK: Penambahan minyak pada tanah umumnya dapat menginduksi ekspresi lipase mikroorganisme lipolitik dalam tanah. Tujuan penelitian ini adalah untuk mengetahui pengaruh penambahan minyak jelantah dan waktu inkubasi serta interaksi antara keduanya terhadap aktivitas lipase pada tanah hutan mangrove Pantai Tablolong Kabupaten Kupang, Nusa Tenggara Timur (NTT). Penelitian ini termasuk dalam True Experiment dengan Rancangan Acak Lengkap (RAL) pola faktorial yang terdiri atas 2 faktor. Faktor pertama adalah waktu inkubasi (tanpa dan dengan inkubasi selama 1, 2, 3, 4, dan 5 hari); dan faktor kedua adalah pengaruh minyak jelantah (tanpa dan dengan penambahan minyak jelantah 1,96; 3,84; dan 5,65% [v/v]). Penentuan aktivitas lipase dilakukan dengan metode titrimetri dan data yang dihasilkan diolah menggunakan metode anova 2 (dua) arah. Hasil penelitian menunjukkan bahwa penambahan minyak jelantah dan waktu inkubasi dapat meningkatkan aktivitas lipase pada tanah hutan mangrove. Minyak jelantah dapat digunakan sebagai penginduksi lipase mikroorganisme lipolitik dalam tanah hutan mangrove, tetapi tidak dapat digunakan lebih tinggi dari 3,84% (v/v). Aktivitas lipase tertinggi diperoleh 1,233 U/mL pada waktu inkubasi selama lima hari. Hasil analisis data menunjukkan tidak ada interaksi antara waktu inkubasi dan penambahan minyak jelantah terhadap aktivitas lipase pada tanah hutan mangrove.ABSTRACT: Addition of oil on soil can usually induce lipase expression of lipolitic microorganism on the soil. The aim of this research was to know the effect of cooking oil waste addition and incubation period varies on the activity of lipase from mangrove forest soil of Tablolong Beach, Kupang, Nusa Tenggara Timur (NTT). This research was in True Experiment with completion random design of factorial model that contains 2 factors. The first factor was incubation period (0, 1, 2, 3, 4, and 5 days); and the second factor was the addition of cooking oil waste (0; 1.96; 3.84; and 5.65% [v/v]). The activities of lipase were determined by titrimetric method. Data of this study was analyzed by using anova two factors. The results of this research showed that the addition of cooking oil waste and incubation period could increase the lipase activity on the mangrove forest soil. The cooking oil waste could be used as inducer of lipase from lipolitic microorganism on mangrove forest soil, but it could not be used more than 3.84% (v/v). The highest lipase activity was 1.233 U/mL on incubation period for five days. The result of data analysis showed no interaction between incubation period and addition of cooking oil waste on lipase activity from mangrove forest soil. 
AKTIVITAS ANTIKANKER ISOLAT TOKSIK DARI EKSTRAK METANOL SPONS GENUS Haliclona Grant, 1836 TERHADAP SEL HELA Ni Made Diani; I Made Dira Swantara; I Gede Mahardika
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 3 No 2 (2015)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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Abstract

ABSTRAK: Telah dilakukan uji aktivitas antikanker isolat toksik dari ekstrak metanol spons genus Haliclona Grant, 1836 terhadap sel HeLa. Uji untuk mengetahui toksisitas spons genus Haliclona Grant, 1836 dilakukan dengan metode Brine Shrimp Lethality Test (BSLT) menggunakan larva Artemia salina Leach terhadap ekstrak etanol dan metanol spons tersebut. Hasil uji menunjukkan ekstrak metanol bersifat lebih toksik dengan nilai LC50 32,36 ppm. Hasil partisi ekstrak metanol menghasilkan ekstrak n-heksana, kloroform dan air. Ekstrak kloroform memiliki toksisitas paling tinggi dengan LC50 64,57 ppm. Ekstrak kloroform dipisahkan dengan kromatografi kolom silika gel menggunakan eluen etil asetat : n-heksana (2 : 8), diperoleh 5 fraksi (F1 – F5). Fraksi satu (F1) memberikan nilai toksisitas paling tinggi dengan LC50 70,79 ppm dan berdasarkan uji fitokimia diduga mengandung senyawa golongan steroid. Isolat toksik (F1) selanjutnya diuji secara in vitro terhadap sel HeLa, namun memiliki daya hambat yang sangat rendah dalam membunuh sel HeLa dengan nilai IC50 sebesar 2187,5 ppm.ABSTRACT: A research to test the anticancer activity toxic isolate of the methanol extract genus sponge of Haliclona Grant, 1836 to HeLa cells was conducted. Preliminary test to determine the toxicity of the ethanol and methanol crude extract genus sponge of Haliclona Grant, 1836 was conducted using Brine Shrimp Lethality Test. The results showed that the methanol extract was more toxic with the LC50 value of 32,36 ppm. Partitions of the methanol extract using n-hexane, chloroform and water were conducted and it was found that the chloroform extract was the most toxic against Artemia salina L. larvae with LC50 of 64,57 ppm. The chloroform extract was separated by column chromatography using ethyl acetate : n-hexane (2 : 8) as eluent and 5 fractions (F1, F2, F3, F4 and F5) were obtained. First fraction (F1) was the most toxic with LC50 of 70,79 ppm. Based on the phytochemical tests the toxic compounds were suspected to be a steroid. Furthermore, the toxic isolates (F1) was tested in vitro against HeLa cells, but it was not able to inhibit the growth and kill HeLa cells with IC50 value of 2187,5 ppm.
PENINGKATAN AKTIVITAS SELULASE PADA TANAH HUTAN MANGROVE PANTAI SUWUNG BALI DENGAN PENGAYAAN SUBTRAT SELULOSA JANUR KELAPA (Cocos nucifera) Ika Kurniawati; I Nengah Wirajana; I Gede Mahardika
Jurnal Kimia (Journal of Chemistry) Vol. 7, No. 1 Januari 2013
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.45 KB) | DOI: 10.24843/JCHEM.2013.v07.i01.p10

Abstract

The aim of this study is to determine the effect of coconut (Cocos nucifera) leaf enrichment to soil of mangrove forest of Bali Suwung coastal on the cellulase activity. The measurement of cellulase activity was conducted by CMC method (Carboxymethyl Cellulose Assay) on soil sample with and without enrichment of coconut leaf substrate in incubation time of 1, 2, 3, and 4 weeks. The results showed that the enrichment increased cellulase activity. In addition, cellulase activited the soil samples, increase with incubation times.  
Total Dan Diferensial Leukosit Babi Landrace Yang Diberi Pakan Eceng Gondok (Eichornia crassipes) Dari Perairan Tercemar Timbal (Pb) Komang Suciani Paramita; I Gede Mahardika; Nyoman Sadra Dharmawan
Buletin Veteriner Udayana Vol. 8 No. 2 Agustus 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

The aim of this study was to determine the effect of water hyacinth (Eichornia crassipes) collected from Lead (Pb) polluted water in feed total and differential leukocyte counts of pigs. This study was an experimental study and done used a completely randomized design. The sample that been used was blood from a total of eight Landrace swines, consisted of four groups: pigs were feed without hyacinth (A), pigs were feed with 2,5% hyacinth (B), pigs were feed with 5% hyacinth (C), and pigs were feed with 7,5% hyacinth (D). Blood were then collected from auricularis superficialis vein. The total and differential leukocytes were checked in the Clinical Pathology Laboratory, Faculty of Veterinary Medicine, Udayana University. Total leukocytes count were measured by auto analyzer Scil Vet ABC (ABC Vet 16p). The blood were also processed for blood smear preparation by Giemsa staining before performing examination by using 100x magnificence under a microscope using straight edge method. Data were then analyzed by one-way ANOVA. The results showed that the administration of water hyacinth (Eichornia crassipes) collected from Lead (Pb) polluted water was not influence the total and differential leukocyte counts in pigs.
SOSIALISASI PENYAKIT CACING PITA DAGING BABI DAN BEBERASAN PADA MASYARAKAT DI DESA BINAAN UNUD, DESA TIANYAR BARAT, KARANGASEM Dharmawan N.S; I M. Damriyasa; I. B.M. Oka; I K. K. Agustina; I. B. N. Swacita; I G. Mahardika
Buletin Udayana Mengabdi Vol 12 No 1 (2013): Volume 12 No.1 – April 2013
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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This Community Service was conducted on August 5th, 2012 in Tianyar Barat village, Karangasem regency inthe form of socialization of taeniosis and cysticercosis on the local community leaders. The aim of this programwas to give knowledge, clinical symptoms, transmission, and prevention of the taeniosis and cysticercosis. Therewere 37 participants who come from representatives of each area. Twenty-seven (72.9%) participants reportedthat at their house they raise pigs, and 11 out of 27 (40.7%) participants reported that the pigs are traditionallyrearing raised without a confinement fence. Free-range pig husbandry system was important risk factors for pigcysticercosis. The activity run smoothly as planned. Socialization of these zoonotic diseases has improved theunderstanding of all participants. At the end of the activity, all of them (100%) reported that they were satisfied;and all of them (100%) also are interested in becoming a volunteer to joint to inform the diseases to other people.
PENGUATAN MANAJEMEN PADANG GEMBALA SAPI PUTIH TARO UPAYA KONSERVASI PLASMA NUTFAH ASLI PULAU BALI I W. Suarna; N.N. Suryani; I G. Mahardika; A.A.A.S. Trisnadewi; K.M. Budiasa
Buletin Udayana Mengabdi Vol 16 No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Sapi putih taro adalah salah satu plasma nutfah yang saat ini mulai mendapat perhatian serius dikalangan ilmuwan dan pemerintah daerah untuk melakukan upaya-upaya perlindungan terhadap kelestariannya. Tata laksana pemberian pakan dan sistem pemeliharaan dikandangkan terus menerus sangat memengaruhi kesehatan dan behavior sapi putih. Menyediakan padang gembalaan untuk ternak akan memberikan kesempatan bagi ternak untuk meningkatkan keberagaman sumber pakan dan melakukan exercise sehingga ternak menjadi lebih sehat. Sebuah FGD telah dilaksanakan untuk meningkatkan kapasitas padang gembala melalui aplikasi teknologi asosiasi tanaman. FGD diikuti oleh perangkat Desa Taro, Manajer dan Staf Yayasan Lembu Putih Taro serta difasilitasi oleh Tim dari Puslitbang Tumbuhan Pakan Universitas Udayana. Hasil FGD menyimpulkan bahwa perbaikan dan peningkatan manajemen padang penggembalaan dan teknologi budidaya tanaman pakan diharapkan menjadi kegiatan prioritas untuk dapat meningkatkan kesehatan sapi putih taro dan menjadi objek wisata yang lebih menarik.
Co-Authors ., PROF.DRS. NENGAH BAWA ATMAJA, M.A A. W. Puger Ade Kiki Sintya Dewi Adnyana I P. G. G. Ameliya, Riska Anak Agung Ayu Sri Trisnadewi Anak Agung Putu Putra Wibawa Andini, Ni Putu Mega Arnawa I W. Bernika J.S. Bulu, Sosiawan CANDRADIARTA I P. M. Darmayasa, Dewa Komang Dewi, Ade Kiki Sintya Dharmawan N.S Dharmawan, I N.S. Djokowoerjo Sastradipradja Dwipayana I K. B Febryanti F. A. G.A.M. KRISTINA DEWI Gertrudis Vebriyanti Kahar Gusti A Malelak Gusti Ayu Mayani Kristina Dewi HARDIAWAN N. D HARDIAWAN N. D. Hartadi W. D. I Dewa Ketut Sastrawidana I G A Surya Utami Dewi I G. Lanang Oka Cakra I G. N. Kayana, I G. N. I Gede Ketut Sri Budarsa I Gede Putu Kawiana I GUSTI AYU MANIK ERMAYANTI I Gusti Lanang Oka I GUSTI LANANG OKA I Gusti Lanang Wiratma I K. E. A., Putrayasa I K. K. Agustina I K. SUMADI I Kadek Swastika I Kadek Yoga Kertiyasa I KETUT ARYANA I Ketut Catur Marbawa I Ketut Mangku Budiasa I Ketut Sumadi I Komang Budaarsa I M. Mudita I M. SUASTA I M. Y., Artawan I Made Adhika I Made Damriyasa I Made Dira Swantara I Made Dwi Setiawan I Made Nuriyasa I Made Rasta I Made Sara Wijana I Made Sara Wijana I N. Suastaka I Nengah Sujaya I Nengah Wirajana I NYOMAN RAI I Nyoman Rai I P. A. Astawa I P. A. Astawa I P. A. Astawa I P. A. ASTAWA I Putu Andre Japani Satya Saputra I Putu Ari Astawa I Putu Suyadnya, I Putu I W BUDIARSA SUYASA I W. Suarna I W. SUDIASTRA I W. SUKANATA I Wayan Sandi Adnyana I Wayan Suarna I Wayan Sujana I. B. Sudana I. M. Mastika I. W. Suama I.G.A.M. ARYASIH I.W Kasa I.W. Kasa IDA AYU ASTARINI Ida Ayu Putu Sri Mahapatni Ida Ayu Putu Widani Sugianingrat Ida Bagus Gaga Partama Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Sudana Ika Kurniawati Indra Arimahayana Iryanti Eka Suprihatin K. Yogi Purnamawati Kadek Karang Agustina Kadek Rihendra Dantes Kartikasari D Kencana Jaya IP.G.A.S Ketut Gede Suryawan Komang Suciani Paramita L. Doloksaribu Luh Dewi Anggreni Lumbansiantar M. M Ariasih M. Sudiana Mahendra Made Antara Made Novia Indriani Magna Anuraga Putra Duarsa N. K. S. P., Dewi N. N. Suryani N. P. Mariani N.N. Suryani N.S. Dharmawan Nasrullah H. I. Ngurah Kayana, I Gusti Ni Luh Putu Sriyani Ni Made Diani Ni Made Indra Wahyuni Ni Made Wedayani Ni Nyoman Ayu Widyasari Ni Nyoman Suryani NI PUTU MARIANI Ni Putu Mega Andini Ni Putu Putri Wijayanti Ni Putu Sarini Ni Putu Sarini Ni Wayan Suniasih Nyoman Sadra Dharmawan Oka A.A P. E. N., Putri P.A. Astawa Perdana, Sigit Pranata I P. Y. A. Prawira I G. I. K. Putu Lakustini Cahyaningrum PUTU SUYADNYA Putu Vindhy Chempaka Putri S. PUTRA S. Putra S. Putra Saransi A. U. Sastrawan I P. L. Sastrawan P. K. P. N SENTANA PUTRA SENTANA PUTRA Sentana Putra Sigit Perdana Simbolon M. Y. Sinta Dewi R. A. Sio Stefanus, Sio Sosiawan Bulu Sri Dian Meita Sari Suasta I M. Suasta IM. Supriana I M. Surya Kencana Jaya P. G. A. Tjokorda Gede Belawa Yadnya Udayana P. W. K. Wayan Andhika Putrayasa Wedayani, Ni Made Widnyani D A. P. Yusadana P.