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Suppementation of Kelor Leaf (Moringa oleifera) Aqueous Extract Increase on Post-Thawed Limousin Bull Sperm Quality Wajdi, Syuhuud Arumbinang; Utomo, Budi; Rimayanti, Rimayanti; Safitri, Erma; Suprayogi, Tri Wahyu; Wurlina, Wurlina
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.249-255

Abstract

The purpose of this research was to determine the best dosage of Moringa oleifera Aqueous extract in egg yolk skim milk extender for post thawed Limousin Bull sperm quality on aspect viability, and the level of. The treatment was divided into five groups: egg yolk and skim milk diluter (P0), 2,5% M. oleifera aqueous ekstract in 4 ml egg yolk skim milk (P1), 5% M. oleifera aqueous ekstract in 4 ml egg yolk skim milk (P2), 10% M. oleifera aqueous ekstract in 4 ml egg yolk skim milk (P3), 20% M. oleifera aqueous ekstract in 4 ml egg yolk skim milk (P4). The sperm quality was observed post thawing. The data were analyzed using ANOVA and Duncant Test. The best sperm motility showed on P2 with 43b ± 5,70, the best sperm viability showed on P3 with 58,20b ± 8,72 and than the lowest level of malondialdehyde showed on P4 with 5,434a ± 1,034. In conclusion addition of M. oleifera on dose 10% can increase quality of Limousin Sperm Post Thawed.
Profile of Progesterone Levels After Administration of Gonadotropins in Dairy Cattle with Ovarian Hypofunction Berliana, Erprinanda Galuh; Utama, Suzanita; Wurlina, Wurlina; Srianto, Pudji; Lestari, Tita Damayanti; Ratnani, Hermin
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.1-5

Abstract

This study aimed to evaluate the profile of progesterone in dairy cattle with ovarian hypofunction. A total of 10 cows in this study were evaluated three times in the collection phase, i.e. (F1) when the cow was diagnosed with ovarian hypofunction, (F2) when the cow was in heat and (F3) 21 days after artificial insemination to detect pregnancy. Samples in the form of blood serum were then analyzed using ELISA. As a result, the average time of heat in ovarian hypofunction cows was 7,4 days. Progesterone levels in F1 were 1,027 ng/ml, 2,774 ng/ml, 1,476 ng/ml, 2,256 ng/ml, 1,258 ng/ml, 1,758 ng/ml, 2,393 ng/ml, 0,592 ng/ml, 0,755 ng /ml, 1,876 ng/ml, respectively. Progesterone levels in F2 were 0,671 ng/ml, 0,517 ng/ml, 0,763 ng/ml, 0,598 ng/ml, 0,615 ng/ml, 0,537 ng/ml, 0,726 ng/ml, 0,643 ng/ml, 0,593 ng/ml, 0,975ng/ml, respectively. Progesterone levels in F3 were 15,642 ng/ml, 4,215 ng/ml, 17,327 ng/ml, 20,721 ng/ml, 5,796 ng/ml, 17,214 ng/ml, 15,815 ng/ml, 16,745 ng/ml, 4,632 ng /ml, 18,281 ng/ml, respectively. The pregnancy rate in hypofunctional cows treated with PG-600 in this study was 70%.
KEJADIAN KAWIN BERULANG PADA SAPI POTONG BETINA PERANAKAN LIMOSIN DAN SIMENTAL DI KECAMATAN WONOSALAM KABUPATEN JOMBANG PERIODE 2015 - 2018 Subagio, Muharam; Triana, Indah Norma; Poernomo, Bambang; Wurlina, Wurlina; Srianto, Pudji; Utomo, Budi
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20412

Abstract

Repeat Breeding is an adult cattle which have normal estrous cycle and clear signs of estrous but did not get pregnant when it got breed by natural mating or artificial insemination. The main purposes of this study is to know the number of repeat breeding cases in beef cattle in regency Jombang period of 2015-2018. The metode of the study is survey which collected by recording and survey. Were analyzed though Normality Test (Kolmogorov-Smirnov Test) and Homogenity Test and followed by T Test. Questionnaire test result processed using Kendall's Tau-b Correlation Test. Study result showed that repeat breeding cases fluctuate significantly per year and the factors affecting fertilization failure and early embrionic death, hormonal, infection, and management factors.   
THE ROLE OF ACUPUNCTURE THERAPY TO OVERCOME ONE-SIDE HEAD PAIN (MIGRAINE) Mainnah, Mut; Septriana, Maya; Wurlina, Wurlina
Journal of Vocational Health Studies Vol. 5 No. 3 (2022): March 2022 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V5.I3.2022.192-202

Abstract

Background: Migraine is a primary headache, usually described as pain attacks lasting 4 - 72 hours, unilateral, throbbing, with moderate to severe pain intensity that can be exacerbated by activity, and can be accompanied by nausea and vomiting, photophobia and phonophobia. Migraine can interfere with patient activities, so that it can reduce productivity and result in an economic burden to the family and reduce the patient's quality of life. To handle it, pharmacological and non-pharmacological therapies can be used. Acupuncture therapy is one of the non-pharmacological therapeutic options that can be used in migraines. Purpose: This article review collects and analyzes journals related to the effect of acupuncture therapy on migraines. The journal search method was carried out electronically from December to April 2021 using several databases such as Pubmed, Sagepub and Google Schoolar with the keywords: acupuncture in cases of migraine, acupuncture for migraine, and acupuncture point for migraine. Review: The journal searches discovered that acupuncture therapy had an effect in reducing pain intensity in migraine cases with the most widely used method that was the manual acupuncture method. However, the most influential method was the combination method of manual acupuncture, herbal acupuncture, and nutritional therapy with the hyperactivity of Yang liver as the frequently occurring syndrome. The frequently used points were the acupuncture points of Fengchi-GB20, Hegu-LI4, Taichong-LR3, Baihui-DU20, Zusanli-ST36, Sanyinjiao-SP6, and Taiyang-EX-HN5.Conclusion: Based on the journal analyses, the combination method of manual acupuncture, herbal acupuncture, and nutritional therapy can reduce pain intensity in migraine cases
White guava (Psidium guajava L.) fruit juice ameliorated the number of spermatogenic cells in rats (Rattus Novergicus) exposed to lead acetate Muhammad Faizal Adiutomo; Suzanita Utama; Herry Agoes Hermadi; Wurlina Wurlina; Hardany Primarizky; Rochmah Kurnijasanti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.39-46

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This study aims to determine the effect of oral administration of white guava fruit juice (Psidium guajava L.) on the spermatogenic cells of rats (Rattus norvegicus) exposed orally to lead acetate. Twenty-five male rats (Rattus norvegicus) of the Wistar strain were randomly divided into five groups. Placebo group rats (P0) were given 0.5 mL distilled water twice every day orally at four-hour intervals. Groups T0, T1, T2, and T3 were given lead acetate 50 mg/kg bw orally, then respectively given distilled water, 100, 50 and 25% (v/v) white guava (Psidium guajava L.) fruit juice orally four hours later for 14 days. On day-15, the rats were sacrificed and their testicles were taken for histological preparations. Data were analyzed using Analysis of Variance followed by Duncan's Multiple Range Test. The results showed that the number of spermatogonia, spermatocytes and spermatids cells of rats in T0 group was lower (p <0.05) than in P0 group. Higher spermatogonia, spermatocytes and spermatids cells (p <0.05) were found in the T1 group compared to the T0 group. However, the number of spermatogonia and spermatocytes in the T1 group was still lower than in the P0 group. The number of spermatids in the T3 group was almost the same (p >0.05) as in the P0 group. This study found that white guava pure juice without dilution was the best for maintaining the number of spermatids, but was unable to restore the number of spermatogonia and spermatocyte cells in rats exposed to lead acetate.
Effect of PG-600 dose in cows with persistent corpus luteum treated with PGF2α and hCG Sofia Brenieta Brilianti; Sri Mulyati; Wurlina Wurlina; Budi Utomo; Oky Setyo Widodo
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.90-95

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The purpose of this study was to determine the effect of administering a dose of gonadotropin (PG-600) in cows with persistent corpus luteum (PCL) which were treated with prostaglandin F2 α (PGF2α) and human chorionic gonadotropin (hCG). This study used 20, three-year-old cows diagnosed with PCL, had recently given birth, and were not pregnant. Cows were randomly allocated into groups T0, T1, T2 and T3.  Cows in all groups were injected with PGF2α (25 mg/cow). Simultaneously, cows in groups T1, T2 and T3 were also injected with PG-600 at doses of 100, 150 and 200 IU/cow respectively. PGF2α and PG-600 were injected when PCL was identified. After that, cows showing signs of estrus were injected with hCG (100 IU/cow) and artificially inseminated 12-18 hours after the appearance of signs of estrus. AI was conducted using frozen thawed semen. Sixty days following AI, a pregnancy examination was conducted using USG. Estrus was detected in all treated cows, while the pregnancy rates varied. Compared with other groups, the groups given 100 and 150 IU PG-600 produced higher pregnancy rates. It could be concluded that the combination of 25 mg PGF2α, 150 IU PG-600, and 100 IU hCG was the most effective treatment to produce high pregnancy rates in cows with persistent corpus luteum.
Fish consumption and dementia prevention in the elderly: Evaluating the "Dudu Galak" program in the coastal community of Bulak, Surabaya [Konsumsi ikan dan pengurangan risiko demensia pada lansia: Implementasi program "Dudu Galak" pada masyarakat pesisir Bulak, Surabaya] Rachmawati, Kadek; Sukmanadi, M; Yudaniayanti, Ira Sari; Wurlina, W; Santoso, Kuncoro P.; Damayanti, Ratna; Putri, Desak Ketut Sekar Cempaka
Buletin Pengabdian Vol 5, No 1 (2025): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpen.v5i1.43101

Abstract

In Indonesia, the elderly population is steadily increasing, and dementia is emerging as a significant public health concern. A lack of awareness regarding dementia prevention, particularly through nutrition-based interventions such as increased fish consumption, remains a major issue. This community service program aimed to educate fishermens wives and elderly residents by promoting the processing of fishery products, especially fish and shellfish, into various nutritious food products as a preventive measure against dementia. The program was implemented in Bulak, a coastal area of Surabaya, Indonesia, targeting fishermens wives and elderly community members through the Dudu Galak Program. The initiative focused on innovating the processing of local marine products into both dry and wet foods that are not only nutritionally rich but also economically valuable. The methods employed included educational outreach, hands-on training, and mentoring in the development of diverse, nutritious, and marketable seafood-based products. Additionally, the program provided education on dementia in older adults and strategies for its prevention. The results of this initiative demonstrated that fishermens wives and elderly residents successfully developed a variety of high-nutrition fish- and shellfish-based foods and snacks with promising market potential. Moreover, participants gained a better understanding of dementia and recognized that increasing fish consumption can serve as an effective preventive strategy. Through this program, it is expected that participants will improve their household income, enhance their knowledge of dementia, and promote greater fish consumption within fishing families as part of dementia prevention efforts among the elderly.
Factors associated with repeat breeding in Holstein Friesian cows at the Tani Wilis Village Cooperative Unit, Sendang District, Tulungagung Regency, Indonesia Dimas Kunto Satrio; M’ Izi Kumala Lazuardi Sultoni; Tasya Apritalia Putri; Soeharsono Soeharsono; Dadik Rahardjo; Djoko Legowo; Gandul Atik Yuliani; Iwan Sahrial Hamid; Mohammad Anam Al-Arif; Sunaryo Hadi Warsito; Pudji Srianto; Sri Pantja Madyawati; Tita Damayanti Lestari; Wurlina Wurlina
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 2 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i2.2025.67-75

Abstract

This study aimed to evaluate the association of factors such as nutrition, thermal humidity index (THI), age, and lactation period with the occurrence of repeat breeding of dairy cows. The sample consisted of Holstein Friesian cows aged 1-11 years, with lactation periods ranging from 1 to 7 months, currently in lactation, and experiencing repeat breeding (inseminated three or more times without conception, despite exhibiting normal estrous cycles and intervals). The results showed that feeding practices by farmers did not significantly differ (p >0.05) in relation to the incidence of repeat breeding. THI values recorded in several barns also showed no significant differences (p >0.05), with the cows experiencing light to moderate heat stress. Similarly, the frequency of artificial insemination and the estrous cycle intervals remained within the normal range. The highest incidence of repeat breeding was observed in cows aged 2.5 to 5.5 years, accounting for 78.13%, and during lactation periods of 1-3 months, accounting for 81.26%. It can be concluded that there were no significant differences in the provision of forage and leguminous feed between normal cows and those with repeat breeding, and the crude protein content of forage did not influence its occurrence. Additionally, there was no indirect relationship between THI and repeat breeding. However, the incidence of repeat breeding tended to increase in cows aged 3-4 years, and during the second and third lactation periods.
PENAMBAHAN L – ARGININ DALAM PENGENCER TRIS KUNING TELUR SETELAH EKUILIBRASI 2 JAM TERHADAP MOTILITAS DAN MEMBRAN PLASMA UTUH SPERMATOZOA DOMBA SAPUDI Suryaningsih, Hastini; Sabdoningrum, Emy Koestanty; Susilowati, Suherni; Sardjito, Trilas; wurlina, wurlina; Damayanti, Tita
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.906 KB) | DOI: 10.20473/ovz.v7i2.2018.86-90

Abstract

This research was aimed to determine the addition of L-arginine on motility and sperm plasma membrane of Sapudi sheep after two hour equilibration time in egg yolk tris diluent. This research used fresh samples of sheep Sapudi semen collected by artificial vagina, then were devided into 4 treatments and 6 replications. Analysis of the data using Analysis of Variance (ANOVA) then proceed to the Duncan Test to determine significant differences between treatments. The first treatment P0 was no L – Arginine added as control. P1 was treated with 0,004 M L – Arginine. P2 was treated with 0,005 M L – Arginine, P3 was treated with 0,006 M L – Arginine. Result of two hour equilibration time for the sperm motility showed: (P0) 46,67a   5,16, (P1) 49,16ab   5,84, (P2) 55,00bc   7,74, (P3) 58,33c   6,83. Result of two hour equilibration time for the sperm plasm membrane were: (P0) 39,33a   4,92, (P1) 43,33ab   4,22, (P2) 46,50 bc   3,44, (P3) 50,83 c   3,48. The addition of L-arginine with a concentration as much as 0.006 M, shows the highest result in sperm motility and sperm plasm membrane intact of Sapudi semen.
PENGARUH PEMBERIAN INSULIN-LIKE GROWTH FACTOR-I (IGF-I) DARI SERUM KUDA CROSSBREED BUNTING TERHADAP FOLIKULOGENESIS MENCIT (Mus musculus) Abdullah, Abdullah; Restiadi, Tjuk Imam; Lastuti, Nunuk Dyah Retno; Damayanti, Tita; wurlina, wurlina; Safitri, Erma
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (526.711 KB) | DOI: 10.20473/ovz.v7i2.2018.102-108

Abstract

The purpose of the research was to know the effect of Insulin-Like Growth Factor-I (IGF-I) derived from pregnant crossbreed mare serum (PMS) in mice (Mus musculus) folliculogenesis. The subject of this research were 20 female mice. The research was arranged by Completely Randomized Design (CRD) with four treatments and five replications. The treatment were K0 = 10 ng/ml of physiological NaCl, P1 = 10 ng/ml of IGF-I PMS, P2 = 20 ng/ml of IGF-I PMS, and P3 = 40 ng/ml of IGF-I PMS. Observed variables are number of primary, secondary, tertiary and de Graff follicles. During the treatment the estrus cycle was also observed. The data of follicles number were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS significantly affect (p<0,05) on increasing of the primary and secondary follicles number. The addition of IGF-I PMS 20 ng/ml and 40 ng/ml can increase the primary and secondary follicle significantly (p<0,05).
Co-Authors Abdul Samik, Abdul Abdullah Abdullah Achmad Basori, Achmad Al arif, Mohammad Anam Alifia, Annisa Ananda, Hanifah Muslimah Andriyanti, Silvia Rani Bambang Poernomo Benjamin Christoffel Tehupuring, Benjamin Christoffel Berliana, Erprinanda Galuh BUDI UTOMO Budi Utomo Budiarto Budiarto Chusniati, Sri Dadik Rahardjo Damayanti, Tita Darsono, Roesno Desak Ketut Sekar Cempaka Putri Desi Wulansari Desi Wulansari Dewa Made Sucipta Putra Dimas Kunto Satrio Djoko Legowo Eka Pramyrtha Hestianah Elok Apriliawati Erma Safitri Fitri, Paraswita Eindah Gandul Atik Yuliani Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hariadi, Mas'ud Hariadi, Masud Harijani, Nenny Hermin Ratnani Herry Agoes Hermadi Hidajati, Nove Hidanah, Sri I Dewa Putu Anom Adnyana I. Mustofa Imam Mustofa IMAM MUSTOFA Indah Norma Triana, Indah Norma Ira Sari Yudaniayanti Iwan Sahrial Hamid Iwan Sahrial Hamid Kadek Rachmawati Kadek Racmawati Lucia Tri Suwanti, Lucia Tri Machmudia, Ainun Mainnah, Mut Mardatillah, Meidy Mardian, Nur Zaini Nanda Mas’ud Hariadi Meles, Dewa Ketut Mufasirin Muhammad Faizal Adiutomo Muhammad Thohawi Elziyad Purnama M’ Izi Kumala Lazuardi Sultoni Ngakan Made Rai Widjaja Ngakan Made Rai Widjaja Ngizzah, Nailul Niluh Suasanti Niluh Suwasanti Nunuk Dyah Retno Lastuti Nurdianto S., Ricko Nurina Hasanatuludhiyah Poedji Hastutiek Prastyorini, Vina Ari Primarizky, Hardany Pudji Srianto Putri, Desak Ketut Sekar Cempaka Putri, Faradillah Hapsari Dwi Racmawati, Kadek Ratna Damayanti Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti Rimayanti Rimayanti Rochmah Kurnijasanti S. Susilowati Sabdoningrum, Emy Koestanti Sabdoningrum, Emy Koestanty Santoso, Kuncoro P. Septriana, Maya Setyadi, Arda Wahyu Siti Nur Fatimah Soeharsono Soeharsono Soeharsono Soeharsono Sofia Brenieta Brilianti Sri Agus Sudjarwo Sri Mulyati Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Subagio, Muharam Suherni Susilowati Sukmanadi, M Sunarni Zakaria Sunarni Zakaria Sunarni Zakaria Supranianondo, Koesnoto Suryaningsih, Hastini Suwasanti, Ni Luh Suwasanti, Niluh Suzanita Utama Swari, Wahyu Retno Syuhuud Arumbinang Wajdi, Syuhuud Arumbinang T. Hernawati Tasya Apritalia Putri Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Ummaisyah, Winadya Reika Wahjuni, Retno Sri Warsito, Sunaryo Hadi Widjiati w Widjiati w Widjiati Widjiati, Widjiati Widodo, Oky Setyo Y. Oktanella Yustinasari, Lita Rakhma