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All Journal ACTA VETERINARIA INDONESIANA Journal Of Vocational Health Studies Tropical Animal Science Journal Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Berkala Penelitian Hayati Jurnal Kedokteran Hewan Buletin Pengabdian
Wurlina, W
Department Of Veterinary Reproduction, Faculty Of Veterinary Medicine, Universitas Airlangga, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Kejadian mastitis subklinis pada kambing perah Peranakan Etawa di desa Bangelan kecamatan Wonosari kabupaten Malang Mardian, Nur Zaini Nanda; Soeharsono, Soeharsono; Harijani, Nenny; Budiarto, Budiarto; Hermadi, Herry Agoes; Wurlina, Wurlina
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.60-63

Abstract

Etawa crossbred goat is one species of goat that is raised for milk production. One of the diseases that often occur in dairy livestock is mastitis, in which there is inflammation of the mammary gland. It is common in goat, that mastitis does not show clinical symptoms (subclinical). This research aims to determine the incidence of subclinical mastitis in Etawa crossbred goats in Bangelan village Wonosari district Malang regency. This research involved 25 respondent farms, which are Etawa crossbred dairy goat farms. In this research, subclinical mastitis cases were directly detected using California Mastitis Test (CMT). The results showed that out of 70 samples collected, 41 (58.6%) samples were positive. These CMT positive goats were from 88% of the respondent farms.
Efektivitas pemberian GnRH pada sapi perah yang mengalami hipofungsi ovarium terhadap waktu timbulnya birahi dan angka kebuntingan Ummaisyah, Winadya Reika; Madyawati, Sri Pantja; Rimayanti, Rimayanti; Wurlina, Wurlina; Restiadi, Tjuk Imam; Wahjuni, Retno Sri
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.64-68

Abstract

Ovarian hypofunction is a condition in which the follicles in the ovary can not develop due to lack of feed. It can affect the pituitary anterior, so the production of FSH and LH was low. GnRH can stimulate the pituitary anterior to produced FSH and LH. This study aimed to prove that GnRH injection in dairy cows with ovarian hypofunction can initiate estrus, followed by pregnancy. This study used 12 dairy cows with BCS ≥ 2.75, with anestrus due to ovarian hypofunction. All dairy cows were divided into two treatment groups, P1 injected with GnRH at a dose of 100 µg intramuscularly and P2 injected with GnRH at a dose of 300 µg intramuscularly. The results were analyzed using an independent t-test and Chi-Square test. The result showed that estrus rate of P1 and P2 were both 100%, meanwhile onset of estrus of P2 (53.83 ± 8.1 h) was shorter p <0.05) than P1 (74.17 ± 4.7 h). Both of P1 and P2 showed 100% pregnancy rate. It could be concluded that GnRH injection could initiate 100% estrus, and followed by 100% pregnancy in dairy cows with ovarian hypofunction.
Efek pemberian α-tokoferol terhadap jumlah sel spermatogenik dan sel Leydig pada tikus putih (Rattus norvegicus) yang dipapar 2,3,7,8 Tetrachlorodibenzo-p-dioxin Machmudia, Ainun; Eliyani, Hana; Widjiati, Widjiati; Wurlina, Wurlina
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 3 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i3.2021.72-80

Abstract

 This research aimed to determine the effect of α-tocopherol on the count of spermatogenic and Leydig cells in rats (Rattus norvegicus) exposed to 2,3,7,8 tetrachlorodibenzo-p-dioxin (dioxin). Dioxin is an endocrine-disrupting agent that adversely affects reproductive health, while α-tocopherol maintains fertility. This research used 25 rats aged 10-12 weeks weighing 150-200 grams. Rats were divided into five groups (K, P0, P1, P2 and P3). The K (control group) was administered with corn oil 1 ml/day. P0 was exposed to 700 ng/kg/day dioxin. P1, P2 and P3 was exposed to dioxin at a dose of 700 ng/kg/day and administered with α-tocopherol at a dose of 77, 140 and 259 mg/kg/day respectively. Dioxin exposure, corn oil and α-tocopherol administration were conducted orally for 20 days. On day-21, all rats were sacrificed for histological slides preparation of testicles with hematoxylin-eosin staining. Data were analyzed with analysis of variance and continued with the Duncan test. The results indicated that exposure to dioxin caused a decrease in the number of spermatogenic and Leydig cells. The administration of α-tocopherol at a dose of 140 mg/kg/day eliminated the effect of reducing the number of spermatogenic and Leydig cells caused by exposure to dioxin. The conclusion was the administration of α-tocopherol at 140 mg/kg/day was effective in maintaining the number of spermatogenic and Leydig cells in rats (Rattus norvegicus) exposed to 2,3,7,8 tetrachlorodibenzo-p-dioxin.
Moringa oleifera leaf extract restored the diameter and epithelium thickness of the seminiferous tubules of rat (Rattus norvegicus) injected with gentamicin Mardatillah, Meidy; Wurlina, Wurlina; Yudaniayanti, Ira Sari; Primarizky, Hardany; Plumeriastuti, Hani; Hamid, Iwan Sahrial
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.15-21

Abstract

This research aims to determine the effect of Moringa oleifera leaf extract (MLE) on the diameter and epithelium thickness of the seminiferous tubules of rats induced with gentamicin. This study used 25 male rats (Rattus norvegicus) aged 8-12 weeks with 150-200 grams body weight. Negative control group (C-) was injected with aquadest and given 0.5% Na-CMC orally, positive control group (C+) was induced with gentamicin injection of 5 mg/kg BW/day and given 0.5% Na-CMC orally, while groups T1, T2, and T3 were induced with gentamicin at the same dose and treated orally with MLE of 200, 316, 500 mg/kg BW/day. Analysis of variance showed significant differences and further analysis using Duncan Multiple Range Test showed difference in each group. The results showed that treatment with MLE of 500 mg/kg BW/day improved the diameter and epithelium thickness of the seminiferous tubules of rats injected with gentamicin. It could be concluded that MLE restored the diameter and epithelium thickness of the seminiferous tubules of rats induced with gentamicin.
Effect of fruit juices in skim milk extender in maintaining Sapudi ram spermatozoa quality at chilled temperature Retta, Ayun Tria Marga; Suherni Susilowati; Sri Pantja Madyawati; Tatik Hernawati; Wurlina Wurlina; Retno Sri Wahjuni
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 2 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i2.2022.49-53

Abstract

This study aimed to determine the effect of the addition of cherry tomato, california papaya, and honey melon juice into skim milk extender in maintaining the quality of Sapudi ram semen at chilled temperature (5oC) storage. Five replication of ejaculates were divided equally into four groups. In control group (T0) semen was diluted in skim milk extender, while in T1, T2, and T3 groups semen were diluted in skim milk extender contained 20% of cherry tomato, california papaya, and honey melon juice, respectively. The extended semen was stored at a chilled temperature, and semen quality (based on sperm motility, viability, and plasma membrane integrity) was observed daily for five days. The result showed that semen quality declined day by day during the five days of storage. Based on the minimum standard of post-thawed semen motility (40%), the spermatozoa of the control group only lasted by the second day. Meanwhile, in the groups with the addition of fruit juice motility could last up to the third day, with the highest motility (p <0.05) on the addition of california papaya juice. This study concluded that the addition of 20% (v/v) california papaya juice in skim milk extender could maintain the percentage of spermatozoa motility up to three days at a chilled temperature.
Effect of ethanolic extract of Moringa oleifera leaves on the number of spermatogenic cells and Leydig cells of gentamicin-induced rats Ngizzah, Nailul; Wurlina Wurlina; Poedji Hastutiek; Iwan Sahrial Hamid; Eka Pramyrtha Hestianah; Lita Rakhma Yustinasari
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.99-106

Abstract

This study aimed to determine the effect of the administration of ethanolic extract of Moringa oleifera leaves on the number of spermatogenic and Leydig cells of gentamicin-induced rats (Rattus norvegicus). This study used 25 white male rats divided randomly into five groups. The rats were injected with Gentamicin 5 mg/kg bw/day subcutaneously for 14 days, except for the negative control group (NCG). During the next 14 days, the rats were given oral moringa leaf extract of 200, 316, and 500 mg/kg bw/day respectively for E200, E316, and E500 groups. The data were analyzed with one-way ANOVA followed by Duncan's test. The results showed that the number of spermatogonia, spermatocytes, and spermatids in NCG was higher (p <0.05) than in the other groups. The number of spermatogonia, spermatocytes, and spermatids in the E316 group was higher (p <0.05) than in PCG. The number of spermatogonia in the E500 group was higher (p <0.05) than in the E316 group, but the number of spermatocytes and spermatids in the E316 group was similar (p >0.05) to the E500 group. The number of PCG Leydig cells was the smallest (p <0.05) compared to the other groups. The administration of Moringa leaves extract in the E200 and E316 groups increased (p <0.05) the number of Leydig cells compared to PCG. The number of Leydig PCG cells in the E500 group was not significantly different (p >0.05) from that of the NCG. It could be concluded that administration of Moringa oleifera leaves extract restored the number of spermatogenic and Leydig cells of gentamicin-induced rats.
Effect of white guava (Psidium guajava L.) fruit juice on the quality of lead acetate induced rats (Rattus norvegicus) spermatozoa Alifia, Annisa; Sri Mulyati; Wurlina Wurlina; Tatik Hernawati; Budi Utomo; Soeharsono Soeharsono; Sri Agus Sudjarwo; Muhammad Thohawi Elziyad Purnama
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 3 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i3.2023.131-140

Abstract

This study aims to determine the effect of the administration of white guava (Psidium guajava L.) fruit juice on spermatozoa plasma membrane integrity (PMI), morphological abnormality, viability, and motility of lead acetate induced rats (Rattus norvegicus). Twenty-five male rats were divided into five groups: NC (negative control) group, rats were administered with distilled water twice daily at four-hour intervals; T0 (positive control) group, rats were administered daily with lead acetate 50 mg/kg bw and distilled water four hours later; T1, T2, and T3 groups, rats were administered daily with lead acetate 50 mg/kg bw and 0.5 mL of 25, 50, and 100% white guava fruit juice four hours later. The treatment of the rats was conducted for 14 days, and on day 15, all rats were sacrificed to assess the spermatozoa quality. Data was analyzed using ANOVA followed by Duncan's multiple range test at a confidence level of 95%. The results showed that exposure to lead acetate (T0) caused lower spermatozoa PMI, viability, and motility as well as higher spermatozoa morphological abnormalities (p <0.05) compared to those of the T0 group. Administration of white guava fruit juice starting at a dose of 25% (T1) resulted in higher spermatozoa motility, viability, and PMI as well as lower spermatozoa morphological abnormalities (p <0.05) compared to rats in the T0 group. It could be concluded that white guava fruit juice maintained the spermatozoa quality of lead acetate induced rats.
IDENTIFIKASI GROWTH DIFFERENTIATION FACTOR-9 (GDF-9) DARI MATURASI IN VITRO OOSIT SAPI DENGAN TEKNIK IMUNOSITOKIMIA w, Widjiati; Wulansari, Desi; w, Wurlina; Widjaja, Ngakan Made Rai
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v5i2.356

Abstract

Penelitian ini bertujuan mengindentifikasi GDF-9 di dalam oosit yang berasal dari preantral folikel sapi yang dimatangkan secara in vitro. Penelitian ini menggunakan metode imunositokimia ikatan antara molekul avidin dan biotin yang terkandung dalam antibodi sekunder bentuk kompleks avidin-biotin. Ovarium sapi diambil dari rumah potong hewan (RPH) Pegirian Surabaya. Penelitian ini menggunakan 105 oosit matang yang berasal dari 62 ovarium. Hasil imunositokimia menunjukkan bahwa 77,14% GDF-9 teridentifikasi.
Peningkatan nilai produk olahan hasil laut dan gerakan gemar makan ikan bagi kelompok istri dan anak nelayan guna mencegah stunting [Increasing the value of processed sea products and the fish-eating movement for groups of fisherman wives and children to prevent stunting] Rachmawati, Kadek; Mulyati, Sri; Utama, Suzanita; Wurlina, W; Santoso, Kuncoro P.; Suwasanti, Ni Luh
Buletin Pengabdian Vol 4, No 1 (2024): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpengmas.v4i1.35366

Abstract

This Community Service Activity is carried out to educate fishermen's wives on how to process marine products such as fish into processed foods that children like so that children will be more fond of eating fish, processed innovations using raw materials from marine products into dry products and wet products with high-quality nutritional value with high selling value, and increasing the love of eating fish among fishing families to increase intelligence and prevent stunting. The methods used are education, training, and mentoring for innovations in processed seafood which are popular with children and have high selling value, apart from that, education is also carried out about stunting and how to prevent stunting. The result of this PKM activity is that fishermen's wives can make various foods and snacks made from marine products that are popular with children and have value for sale so that they can provide income for fishermen's wives, as well as increase the understanding of fishermen's wives about stunting to prevent the incidence of stunting in fishermen's children and increase the movement for eating fish among fishermen's families.
Keberlanjutan UMKM olahan hasil laut di Pesisir Bulak Surabaya guna meningkatkan daya saing di era new normal (Sustainability of MSMEs processed marine products in Bulak Coastal Surabaya to increase competitiveness in the new normal era) Wurlina, W; Racmawati, Kadek; Utama, Suzanita; Mahasri, Gunanti; Mulyati, Sri; Suwasanti, Niluh
Buletin Pengabdian Vol 2, No 2 (2022): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpengmas.v2i2.25217

Abstract

The Regional Leading Product Development Program (RLPDP) activities are carried out to sustain the existence of Micro, Small, and Medium Enterprises (MSMEs) processed marine products in increasing marketing in the new normal of the Covid-19 Pandemic period. This makes the implementation team assist the strategies used by MSMEs and the effectiveness of these strategies. In this activity, the factors influencing MSMEs sustainability were product innovation, packaging, and marketing. The method used was education, training, and mentoring for the innovation of processed seafood using packaging according to its function and marketing. The result of RLPDP was MSMEs have implemented innovations in processed marine products with packaging accompanied by the length of use, labeling, business permits, and halal accompanied by slogans. Marketing of seafood products processing was carried out through the Surabaya UKM center, namely the Siola MSMEs Center, the SIB (Bulak Fish Center) MSMEs, Merr MSMEs, hotels, and participation in exhibition activities organized by the Surabaya City Government and East Java Province. Furthermore, to expand marketing reach, socialization has been carried out through social media such as WhatsApp, Facebook, Instagram, and e-mail, as well as the marketplaces such as Tokopedia, Bukalapak, and Blibli.
Co-Authors Abdul Samik, Abdul Abdullah Abdullah Achmad Basori, Achmad Al arif, Mohammad Anam Alifia, Annisa Ananda, Hanifah Muslimah Andriyanti, Silvia Rani Bambang Poernomo Benjamin Christoffel Tehupuring, Benjamin Christoffel Berliana, Erprinanda Galuh BUDI UTOMO Budi Utomo Budiarto Budiarto Chusniati, Sri Dadik Rahardjo Damayanti, Tita Darsono, Roesno Desak Ketut Sekar Cempaka Putri Desi Wulansari Desi Wulansari Dewa Made Sucipta Putra Dimas Kunto Satrio Djoko Legowo Eka Pramyrtha Hestianah Elok Apriliawati Erma Safitri Fitri, Paraswita Eindah Gandul Atik Yuliani Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hariadi, Mas'ud Hariadi, Masud Harijani, Nenny Hermin Ratnani Herry Agoes Hermadi Hidajati, Nove Hidanah, Sri I Dewa Putu Anom Adnyana I. Mustofa Imam Mustofa IMAM MUSTOFA Indah Norma Triana, Indah Norma Ira Sari Yudaniayanti Iwan Sahrial Hamid Iwan Sahrial Hamid Kadek Rachmawati Kadek Racmawati Lucia Tri Suwanti, Lucia Tri Machmudia, Ainun Mainnah, Mut Mardatillah, Meidy Mardian, Nur Zaini Nanda Mas’ud Hariadi Meles, Dewa Ketut Mufasirin Muhammad Faizal Adiutomo Muhammad Thohawi Elziyad Purnama M’ Izi Kumala Lazuardi Sultoni Ngakan Made Rai Widjaja Ngakan Made Rai Widjaja Ngizzah, Nailul Niluh Suasanti Niluh Suwasanti Nunuk Dyah Retno Lastuti Nurdianto S., Ricko Nurina Hasanatuludhiyah Poedji Hastutiek Prastyorini, Vina Ari Primarizky, Hardany Pudji Srianto Putri, Desak Ketut Sekar Cempaka Putri, Faradillah Hapsari Dwi Racmawati, Kadek Ratna Damayanti Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti Rimayanti Rimayanti Rochmah Kurnijasanti S. Susilowati Sabdoningrum, Emy Koestanti Sabdoningrum, Emy Koestanty Santoso, Kuncoro P. Septriana, Maya Setyadi, Arda Wahyu Siti Nur Fatimah Soeharsono Soeharsono Soeharsono Soeharsono Sofia Brenieta Brilianti Sri Agus Sudjarwo Sri Mulyati Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Subagio, Muharam Suherni Susilowati Sukmanadi, M Sunarni Zakaria Sunarni Zakaria Sunarni Zakaria Supranianondo, Koesnoto Suryaningsih, Hastini Suwasanti, Ni Luh Suwasanti, Niluh Suzanita Utama Swari, Wahyu Retno Syuhuud Arumbinang Wajdi, Syuhuud Arumbinang T. Hernawati Tasya Apritalia Putri Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Ummaisyah, Winadya Reika Wahjuni, Retno Sri Warsito, Sunaryo Hadi Widjiati w Widjiati w Widjiati Widjiati, Widjiati Widodo, Oky Setyo Y. Oktanella Yustinasari, Lita Rakhma