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Journal : Pro-Life

PERTUMBUHAN TUNAS ANGGREK DENDROBIUM SP. MENGGUNAKAN KOMBINASI BENZYL AMINO PURIN (BAP) DENGAN EKSTRAK BAHAN ORGANIKPADA MEDIA VACIN AND WENT (VW) Tia Setiawati
Jurnal Pro-Life Vol. 3 No. 3 (2016): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

The purpose of this study was to get the best combination of BAP with organic material extract that can enhance the growth of Dendrobiumsp shoot in the VW media. The method used was experimental with completely randomized design, which consists of 12 treatments namely combination of BAP concentration with organic material extract. BAP concentration consisted of 4 levels i.e. 1 ppm, 2 ppm, 3 ppm and 4 ppm. The organic material extract used consisted of 3 types of extracts namely tomato extract, corn extract and banana extract, each of 100 mL/L. Each treatment was repeated 3 times. Data were analyzed using Analysis of Variance (Anova) and Duncan 's multiple range test. The results showed that the average of the fastest shoots emergence time obtained on combination of BAP 2 ppm + banana extract was 1.33 days after planting, the average of the highest shoot number was 14 buds obtained on the combination of BAP 3 ppm + tomato extract, and the average of the highest shoots length was 2.06 cm obtained on a combination of BAP 2 ppm + tomato extract. Keywords : BAP, Dendrobium sp., Extract, VW
STUDI MORFOLOGI BEBERAPA JENIS LAMUN DI PANTAI TIMUR DAN PANTAI BARAT, CAGAR ALAM PANGANDARAN Tia Setiawati; Maryam Alifah; Asep Zainal Mutaqin; Mohamad Nurzaman; Budi Irawan; Ruly Budiono
Jurnal Pro-Life Vol. 5 No. 1 (2018): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Sea grass is one component of biodiversity in marine waters composed of many species with various morphological characters. The aim of this study was to obtain the morphological data of seagrass species on the East and West Coasts in Nature Reserve of Pangandaran. The method used was survey method and line transect as far as 200 meters with random sampling technique. Measurements were made for each species of seagrass in the leaf length, leaf width, leaf thickness, stem length and rhizomes length and root length. The results showed that on the East Coast founded three species of seagrass were Cymodocea rotundata, Thalassia hemprichii and Halodule pinifolia, while on the West Coast founded one species of seagrass was Thalassia hemprichii. Cymodocea rotundata has characteristic rounded edges with slight curvature in the middle, Thalassia hemprichii has rounded, brown tip and a brown patterned rhizome, and Halodule pinifolia has side toothed leaf tip with part of the middle there are brown leaf veinsKey words: sea grass, morphology, Pangandaran Nature Reserve
RESPON PERTUMBUHAN STEK CABANG BAMBU AMPEL KUNING (Bambusa vulgaris Schard.Ex Wendl.var. Striata) DENGAN PEMBERIAN ZAT PENGATUR TUMBUH NAA (Naphthalein Acetic Acid) DAN Rootone F Tia Setiawati; Noviyanti Soleha; Mohamad Nurzaman
Jurnal Pro-Life Vol. 5 No. 3 (2018): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Yellow ampel bamboo is one type of bamboo that has economic value. One effort that can be done for maintaining the existence of bamboo population so that its can be sustainably used through the cultivation of branch cuttings. The success growth of branch cutting can improve with using plant growth regulator (PGR), such as NAA and Rootone F. The aim of this study was to get the best kind and concentration of PGR that can promote the growth of ampel yellow bamboobranch cutting. This study used experimental methods with a completely randomized design (CRD) two factors. The first factor wasthe kind of PGR which consists of two levels: z1 = NAA and z2 = Rootone F. The second factor was the concentration of PGR which consists of six levels: k1 = 0 ppm, k2 = 100 ppm, 200 ppm = k3, k4 = 300 ppm, 400 ppm = k5, and k6 = 500 ppm. Data were analyzed using ANOVA (α = 5%) and to know the difference between treatments used Tukey test (α= 5%). The results showed that the Rootone F had a better effect than NAA on the growth of yellow bamboo branch cutting. Concentration of 200 ppm was the best concentration to the growth for branch cuttings of yellow ampel bamboo. Interaction Rootone F and concentration 200 ppm showed the best result to the growth for branch cuttings ampel bamboo.Keywords :branch cutting, ampel yellow bamboo, NAA, Rootone F
KARAKTERISTIK STOMATA BERDASARKAN ESTIMASI WAKTU DAN PERBEDAAN INTENSITAS CAHAYA PADA DAUN Hibiscus tiliaceus Linn. DI PANGANDARAN, JAWA BARAT Tia Setiawati; Inneke Febrihardianti Syamsi
Jurnal Pro-Life Vol. 6 No. 2 (2019): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Hibiscus tiliaceus L. is useful as shade plants, medicinal, and wood carvings. Optimizing utilization of plants depends on environmental conditions, one of which is sunlight that affects stomatal. Each plant has a different response to the stress of sunlight such as the ability to open and close the stomatal. This study aims to determine the differences in the stomatal characteristics of Hibiscus tiliaceus’s leaves based on time estimation and difference of light intensity were determined using the replica method in Pananjung Pangandaran Nature Reserve, West Java. The results showed that the lowest and highest average length and width of porous stomatal, the number of open and closed stomatal, and the density of stomatal in shaded areas were 6 µm (morning and noon), 2.77 µm (afternoon), 6, respectively. 93 µm (morning), 3.83 µm (afternoon), 43 cells, 8 cells, and 259.86 cells / mm2, whereas in the non-shaded area 5.5 µm (morning), 2.97 µm (afternoon ), 7 µm (day and evening), 4.1 µm (afternoon), 18 cells, 26 cells, and 224.20 cells / mm2. The opening of stomatal in the non-shaded area is greater than the shaded. The estimated time, difference light intensity, and physical factors influence the parameters of the study. Keywords: Hibiscus tiliaceus L., light intensity, stomatal characteristics, time estimation.
INDUKSI KALUS KRISAN (Chrysanthemum morifolium Ramat var. Tomohon Kuning) DENGAN 2,4-Dichlorophenoxyacetic Acid (2,4-D) DAN 6-Benzylaminopurine (BAP) PADA KONDISI PENCAHAYAAN BERBEDA Tia Setiawati; Annisa Nur Arofah; Mohamad Nurzaman
Jurnal Pro-Life Vol. 7 No. 1 (2020): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

This study aims to obtain the optimum concentration of 2,4-D and BAP plant growth regulators (PGRs) to induceChrysanthemum callus in light and dark conditions. The method used is an experimental method in the laboratoryusing a Completely Randomized Design (CRD) with 4 treatment of 2,4-D + BAP concentration combinations. Theculture was incubated under different lighting conditions for 45 days after planting. The parameters observedincluded ctexture and color of callus, other responses produced by explants, size, fresh weight and dry weight ofcallus. Data were analyzed descriptively. The results showed that 4 ppm 2,4-D + 0.5 ppm BAP treatment was thebest combination in inducing Chrysanthemum callus in both light and dark conditions. In bright conditions, mostof the callus were dark green and dark brown with a compact texture, callus size of 1.36 cm, and the highest freshweight and dry weight of callus were 0.62 gram and 0.17 gram respectively. Meanwhile, in the dark conditionsmost of the callus were light green and light brown with a compact texture, callus size 1.18 cm, and the highestfresh weight and dry weight of the callus produced were 0.51 grams and 0.15 grams, respectively. Keywords: Callus, Chrysanthemum morifolium, 2,4-Dichlorophenoxyacetic acid (2,4-D), 6-Benzylaminopurine(BAP)
RESPON PERTUMBUHAN KAPULAGA LOKAL (Amomum compactum Soland Ex. Maton) DAN KAPULAGA SABRANG (Elettaria cardamomum (L.) Maton Var. Mysore) TERHADAP CEKAMAN KEKERINGAN Mohamad Nurzaman; Sri Ratu Dewi Pridani; Tia Setiawati
Jurnal Pro-Life Vol. 7 No. 1 (2020): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Drought stress is an inhibiting factor in agricultural, one of the effort to overcome these problems is to use varietiesof plants that are tolerant to drought stress. Cardamom is a plant of the Zingiberaceae which is able to grow indrought stress. This research aims to obtain the growth response of local Javanese cardamom (A. compactum)and true cardamom (E. cardamomum) and to obtain the kind of cardamom that is tolerant to drought stress. Thisresearch was conducted using experimental method and completely randomized design (CRD) with 2 x 4 factorialand four repetitions. The first factor was the variation of field capacity (K) and the second factor was the type ofcardamom (J). Parameters measured include the increase of plant height (cm), plant weight (grams), leaf area(cm2), number of leaves, leaf chlorophyll content (mg / l), and leaf proline content (ppm). The data was analysedusing analysis of variance (ANOVA) and Duncan test α 5%. Results showed that local Javanese cardamom (A.compactum) has better growth response and was more tolerant to drought stress compared to true cardamom (E.cardamomum). Keywords: drought stress, local Javanese cardamom (A. compactum), true cardamom (E. cardamomum).
ANALISIS PERTUMBUHAN DAN KANDUNGAN TOTAL FLAVONOID KULTUR KALUS KRISAN (Chrysanthemum morifolium Ramat) DENGAN PEMBERIAN ASAM 2,4-DIKLOROFENOKSIASETAT (2,4-D) DAN AIR KELAPA Tia Setiawati; Anggita Levi Astuti; Mohamad Nurzaman; Nining Ratningsih
Jurnal Pro-Life Vol. 8 No. 1 (2021): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Chrysanthemum morifolium contains flavonoids which have antioxidant properties and was useful in treating various diseases. The production of flavonoids from plants can be done in vitro through callus culture. Auxin 2,4-D growth regulators and organic compounds such as coconut water added to the media can affect the success of callus formation and secondary metabolite synthesis. The purpose of this study was to obtain the best treatment of 2,4-D concentration and coconut water that can increase the growth and total flavonoid levels of C. morifolium callus cultures. The method used in this research was the experimental method completely randomized design (CRD) with 8 treatments, namely 1 ppm 2,4-D + 10% coconut water (CW); 2 ppm 2,4-D + 10% CW; 3 ppm 2,4-D + 10% CW ; 4 ppm 2,4-D + 10% CW; 1 ppm 2,4-D; 2 ppm 2,4-D; 3 ppm 2,4-D; 4 ppm 2,4-D in 4 replication. The results showed that the treatment of 4 ppm 2,4-D and 10% CW was the best treatment combination, resulting in an average callus size (1.4 cm), fresh weight (0.19 grams), dry weight (0.16 gram) and total flavonoid content (1.873 mgQE/g)
Pemberian Asam Humat untuk Meningkatkan Pertumbuhan dan Kandungan Total Flavonoid Sawi Hijau (Brassica juncea L.) pada Perbedaan Kadar Salinitas Tia Setiawati; Tentani Buhti Amadea; Mohamad Nurzaman; Nining Ratningsih
Jurnal Pro-Life Vol. 8 No. 3 (2021): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Salt affects plant growth due to increased soil osmotic pressure and plant nutrients disturbance. Most plants are susceptible to high salinity which can cause physiological and biochemical interference. This research was conducted to obtain a possible salinity level for mustard greens (Brassica juncea L.) to grow well and the best humic acid dosage to reduce salinity damages and to gain high total flavonoid content. This research used Randomized Block Design method with two factors and four replications. The factors are salinity levels (control, 50, 75, and 100 mM) and humic acid dosage (control, 2, 4, and 8 g/kg). In the parameters which had interactions between the two treatments, the highest results were obtained with a combination of 100 mM salinity and 12 g humic acid, such as plant height (33.35 cm), leaf area (379.66 cm2), fresh weight (22.41 g) and dry weight (1.54 g). The highest results on leaf number (8.5 leaves) and total flavonoid content (0.074 mgQE/g sample) were obtained with 100 mM salinity. Humic acid dose of 12 g gave the highest result on leaf number (8.69 leaves) and total flavonoid content (0.095 mgQE/g sample).
Aplikasi Asam Humat dan Pupuk Organik Cair Ekstrak Rumput Laut untuk Meningkatkan Pertumbuhan dan Kandungan Flavonoid Brassica juncea L. Setiawati, Tia; Fitria, Sania Septiani; Nurzaman, Mohamad; Hasan, Rusdi; Mutaqin, Asep Zainal; Lestari, Ani
Jurnal Pro-Life Vol. 11 No. 2 (2024): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

This research was conducted with the aim of obtaining the optimal dose of humic acid (HA) and liquid organic fertilizer (LOF) from seaweed extract that can enhance the growth and total flavonoid content of Brassica juncea L. The method used in this study was experimental with a randomized complete block design with two factors. The first factor was HA, consisting of 4 dose levels: 0, 4, 8, and 12 g/kg, while the second factor was LOF from seaweed extract, consisting of 4 dose levels: 0, 1, 2, and 3 mL/L. Each treatment was replicated four times. Parameters were observed on the 42nd day, including plant height, leaf number, leaf area, dry weight, and total flavonoids. Data were analyzed using Analysis of Variance (ANOVA) and followed by Duncan's Multiple Range Test with a confidence level of 95%. The results showed that the dose of 4 g/kg HA and 2 mL/L LOF was the best dose for enhancing growth and total flavonoid content, producing an average plant height of 19.25 cm, a leaf number of 5.75, a total leaf area of 179.8 cm2, a dry weight of 0.57 grams, and a total flavonoid content of 0.106 mg QE/g sample.
Karakteristik Kalus dari Eksplan Batang Planlet Krisan (Chrysanthemum morifolium Ramat) pada Media dengan Konsentrasi 2,4-Dichlorophenoxyacetic Acid (2,4-D) dan 6-Benzylaminopurine (BAP) serta Kondisi Pencahayaan Setiawati, Tia; Arofah, Annisa Nur; Lestari, Ani; Hasan, Rusdi
Jurnal Pro-Life Vol. 11 No. 3 (2024): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v11i3.6404

Abstract

This research aimed to obtain the optimal concentration combination of 2,4-D and BAP growth regulators for inducing callus from the stem explant of Chrysanthemum (C. morifolium Ramat) in bright and dark conditions. Stem explants of chrysanthemum were grown in culture medium supplemented with various concentrations and combinations of 2,4-D and BAP under different lighting conditions for 45 days after culture. A laboratory-scale experimental method was used in this research using Completely Randomized Design (CRD). The observed parameters were induction time, percentage formation, size, fresh weight, dry weight, texture, color, and various respones generated by callus. All data were analyzed descriptively. The results showed that all concentrations and combinations of growth regulators could induce callus. In bright condition, the fastest callus induction time was 7 days after culture; most calli were dark green and dark brown colored with compact texture; the callus size was 1.36 cm; also, the highest dry weight generated by callus was 0.17 gram. Meanwhile, in dark conditions, the fastest callus induction time was 6 days after culture; most calli were light green and light brown colored with compact texture; the callus size was 1.18 cm; and the highest dry weight generated by callus was 0.15 gram.
Co-Authors , ,. Mulyadi , ,. Suryana Adityawan, Mohammad Bagus Adnin Laila Agung Karuniawan Agus Irpan Nurhidayat Agustine, Leony Alfira, Ajeng Alma Ayalla Amala Lastari Utami Anandira Witri Andiana, Anisa Andri Irawan Anggita Levi Astuti Anggita Leviastuti Ani Lestari Ani Lestari Anisaa Fauziyah Annisa Annisa Annisa Annisa Annisa Annisa Annisa Nur Arofah Arianti, Tanti Arofah, Annisa Nur Asep Zainal Mutaqin Astuti, Sipa Dwi Auliya Zahra Awali, Dian Siti Ayalla, Alma Azifah An’amillah Bari, Ichsan Budi Irawan Budi Irawan Darmawan, Darwis Debby Ustari Dede Winingsih Delisa Lestari Desak Made Malini Devitri Amisa Dian Latifa Ela Noviani Elah Karimah Eli Lusiani Endang Rosdiana Fadhilla Ramadhona Farhurohman, Oman Fatmawati, Ariani Fikriyah, Nanda adzka Fitria, Sania Septiani Fredella Josenia, Carlen Harkunti P. Rahayu, Harkunti P. Hendi N Wicaksono Heri Kurniawan, Heri Hernandi Sujono Huda, Ida Nurul Ibadurrahman, Rifki Inggriane Puspita Dewi, Inggriane Puspita Inneke Febrihardianti Syamsi Irma Wahyuningsih Irvan Herdiana Jannah, Isma Aenul Johan Iskandar Joko Kusmoro Kartiawati Alipin Kartiawati Alipn Kharismala R, Nurlaila Lia Dewi Juliawaty Libriati, Rika Lusiani, Eli Maitala Sari Marcelline, Karen Marheny Lukitasari Marheny Lukitasari Maryam Alifah Maulani, Susan Maulidiyah Maulidiyah Maya Amalia Meilinda, Pudji Melina Melina Moahamad Nurzaman Moahamad Nurzaman, Moahamad Moch. Nurzaman Mohamad Nurzaman Mohammad Farid Muhammad Syahril Badri Kusuma, Muhammad Syahril Badri Nia Rossiana Nining Ratningsih Nisa Hurin Nisa Hurin Nopriyeni Nopriyeni Nopriyeni, Nopriyeni Noviani, Ela Noviyanti Soleha Nuriman, Muhammad Nurullia Fitriani Nurullia Fitriani Otih Rostiana Putri, Deviana Aulia Rachma, Nadhira Zaachrany Radewi Safira Fauzia Radewi Safira Fauzia, Radewi Safira Rahmawati, Fitryasari Rani Rubiyanti, Rani Rasmiaditya Silasari Revita Yanuarsari Rezekikasari, Rezekikasari Rike Yuniaris Rully Budiono Ruly Budiono Rusdi Hasan Rusdi Hasan Rusdi Hasan Saeful Amin, Saeful Senadi Budiman Sephianti, Selvi Siti Mariyani Sofiyah, Yusi Sri Mulyati Sri Ratu Dewi Pridani Sugarti, Dini Sukrido Sukrido Supriatun, T Syifa Fauzia Zazuli T Supriatun Tentani Buhti Amadea Titin Supriatun Titin Supriatun Titin Supriatun Titin Supriatun Sadeli Valentina Adimurti Kusumaningtyas Vera Octavia Subardja Washfanisa, Handina Alya Wibowo, Sigit Sapto Wicaksono, Hendi N Winingsih, Dede Yana Maolana Syah Yana Maolana Syah Yani Astuti Yuliana, Trisna Yuniar, Muthya Yuniaris, Rike Yusi Sofiyah