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All Journal Jurnal Ilmu Dasar Jurnal Florea Jurnal Teknik Sipil Pro-Life Jurnal Bioedukasi Kultivasi Jurnal Biodjati Jurnal Biologi Tropis Sains dan Matematika Indonesian Journal of Global Health research Indonesian Journal of Adult and Community Education (IJACE) Journal of Comprehensive Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Keperawatan 'Aisyiyah Indonesian Research Journal on Education Manilkara: Journal of Bioscience Calakan: Jurnal Sastra, Bahasa, dan Budaya Jurnal Inovasi Bahan Lokal dan Pemberdayaan Masyarakat
Tia Setiawati
Program Studi Biologi FMIPA Universitas Padjadjaran
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Journal : Pro-Life

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Aplikasi Asam Humat dan Pupuk Organik Cair Ekstrak Rumput Laut untuk Meningkatkan Pertumbuhan dan Kandungan Flavonoid Brassica juncea L. Setiawati, Tia; Fitria, Sania Septiani; Nurzaman, Mohamad; Hasan, Rusdi; Mutaqin, Asep Zainal; Lestari, Ani
Jurnal Pro-Life Vol. 11 No. 2 (2024): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

This research was conducted with the aim of obtaining the optimal dose of humic acid (HA) and liquid organic fertilizer (LOF) from seaweed extract that can enhance the growth and total flavonoid content of Brassica juncea L. The method used in this study was experimental with a randomized complete block design with two factors. The first factor was HA, consisting of 4 dose levels: 0, 4, 8, and 12 g/kg, while the second factor was LOF from seaweed extract, consisting of 4 dose levels: 0, 1, 2, and 3 mL/L. Each treatment was replicated four times. Parameters were observed on the 42nd day, including plant height, leaf number, leaf area, dry weight, and total flavonoids. Data were analyzed using Analysis of Variance (ANOVA) and followed by Duncan's Multiple Range Test with a confidence level of 95%. The results showed that the dose of 4 g/kg HA and 2 mL/L LOF was the best dose for enhancing growth and total flavonoid content, producing an average plant height of 19.25 cm, a leaf number of 5.75, a total leaf area of 179.8 cm2, a dry weight of 0.57 grams, and a total flavonoid content of 0.106 mg QE/g sample.
Karakteristik Kalus dari Eksplan Batang Planlet Krisan (Chrysanthemum morifolium Ramat) pada Media dengan Konsentrasi 2,4-Dichlorophenoxyacetic Acid (2,4-D) dan 6-Benzylaminopurine (BAP) serta Kondisi Pencahayaan Setiawati, Tia; Arofah, Annisa Nur; Lestari, Ani; Hasan, Rusdi
Jurnal Pro-Life Vol. 11 No. 3 (2024): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v11i3.6404

Abstract

This research aimed to obtain the optimal concentration combination of 2,4-D and BAP growth regulators for inducing callus from the stem explant of Chrysanthemum (C. morifolium Ramat) in bright and dark conditions. Stem explants of chrysanthemum were grown in culture medium supplemented with various concentrations and combinations of 2,4-D and BAP under different lighting conditions for 45 days after culture. A laboratory-scale experimental method was used in this research using Completely Randomized Design (CRD). The observed parameters were induction time, percentage formation, size, fresh weight, dry weight, texture, color, and various respones generated by callus. All data were analyzed descriptively. The results showed that all concentrations and combinations of growth regulators could induce callus. In bright condition, the fastest callus induction time was 7 days after culture; most calli were dark green and dark brown colored with compact texture; the callus size was 1.36 cm; also, the highest dry weight generated by callus was 0.17 gram. Meanwhile, in dark conditions, the fastest callus induction time was 6 days after culture; most calli were light green and light brown colored with compact texture; the callus size was 1.18 cm; and the highest dry weight generated by callus was 0.15 gram.
Karakteristik Morfo-Anatomi serta Kandungan Klorofil dan Kandungan Fitokimia Tanaman Ketul (Bidens pilosa) pada Tempat dengan Intensitas Cahaya yang Berbeda di Arboretum Universitas Padjadjaran Marcelline, Karen; Nurzaman, Mohamad; Setiawati, Tia
Pro-Life Vol. 12 No. 1 (2025): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bidens pilosa is a plant known for its secondary metabolites with therapeutic properties. This study aimed to examine the effects of different light intensities on the morphology, anatomy, chlorophyll content, and secondary metabolite production of B. pilosa in the Arboretum of Universitas Padjadjaran. The shaded and unshaded area had light intensities of 5828 and 32768 lux, respectively. Leaf samples were collected from three different individuals in each area, with three leaves taken per plant. Morphological and anatomical traits such as leaf thickness, leaf area, stomatal density, and chlorophyll content were observed, along with secondary metabolite content. Results showed that plants in the shaded area had thinner leaves (0,19 mm) and lower stomatal density (275,16 cells/mm2) but a larger leaf area (4 cm2) and higher chlorophyll content (29,33 CCI) compared to leaves in the unshaded area, which had thicker leaves (0,213 mm), higher stomatal density (310,83 cells/mm2), a smaller leaf area (2,33 cm2), and lower chlorophyll content (27,27 CCI). Alkaloids, flavonoids, tannins, and saponins were detected in both conditions, with a higher level of alkaloid and tannin in unshaded plants. These findings enhance our understanding of the relationship between light intensity influences plant morphology, photosynthesis, and secondary metabolite production, with potential applications in cultivation and medicinal use.
Co-Authors Adityawan, Mohammad Bagus Agung Karuniawan Agustine, Leony Andiana, Anisa Andri Irawan Ani Lestari Arofah, Annisa Nur Asep Zainal Mutaqin Astuti, Sipa Dwi Awali, Dian Siti Ayalla, Alma Bari, Ichsan Debby Ustari Desak Made Malini Fatmawati, Ariani Fitria, Sania Septiani Harkunti P. Rahayu, Harkunti P. Heri Kurniawan, Heri Ibadurrahman, Rifki Inggriane Puspita Dewi, Inggriane Puspita Irvan Herdiana Jannah, Isma Aenul Joko Kusmoro Kharismala R, Nurlaila Libriati, Rika Lusiani, Eli Marcelline, Karen Maulani, Susan Maya Amalia Meilinda, Pudji Mohamad Nurzaman Mohammad Farid Muhammad Syahril Badri Kusuma, Muhammad Syahril Badri Nopriyeni, Nopriyeni Noviani, Ela Nuriman, Muhammad Putri, Deviana Aulia Rachma, Nadhira Zaachrany Rahmawati, Fitryasari Rani Rubiyanti, Rani Rasmiaditya Silasari Revita Yanuarsari Rezekikasari, Rezekikasari Ruly Budiono, Ruly Rusdi Hasan Saeful Amin, Saeful Sofiyah, Yusi Sugarti, Dini Supriatun, T Titin Supriatun Valentina Adimurti Kusumaningtyas Washfanisa, Handina Alya Wibowo, Sigit Sapto Wicaksono, Hendi N Winingsih, Dede Yuniar, Muthya Yuniaris, Rike