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Terapi Ammonium Khlorida-Asam Askorbat untuk Menurunkan Tingkat Keasaman Urin dan Kristalisasi Struvit pada Kucing Urolithiasis (THERAPY AMMONIUM CHLORIDE-ASCORBIC ACID FOR LOWERING URINE ACIDITY AND STRUVITE CRYSTALLIZATION IN FELINE UROLITHIASIS) Alfarisa Nururrozi; Soedarmanto Indarjulianto; Yanuartono Yanuartono; Hary Purnamaningsih; Sitarina Widyarini; Slamet Raharjo; Dhasia Ramandani
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.516 KB) | DOI: 10.19087/jveteriner.2019.20.1.8

Abstract

Struvite/Magnesium ammonium phosphate (MAP) is common causes of feline urolithiasis. Prevent the formation of MAP crystallization can be treated by adjusting the pH urine in acid conditions. Urine with an acid pH will decrease struvite activity product (SAP) by preventing the phosphate deprotonation process which is the main constituent of struvite components. This study aims to determine the potential of a combination of ammonium chloride (NH4Cl) and ascorbic acid to decrease SAP by retain the urine pH under normal conditions. Twelve male cats, 2-5 years old and body weight 3.0 ± 0.8 kg were diagnosed struvite urolithiasis used for this study. Cats have a9clinical history of hematuria, dysuria, polyuria, and stranguria. Diagnosis of urolithiasis performed by clinical examination, USG, x-rays, and urinalysis. Cats that found struvite urolith, treated with 200 mg/kg NH4Cl and 100 mg/kg ascorbic acid orally twice a day. The research objects were observed included urine pH and crystaluria density. The data were analyzed using analisis of varian. The result of this study showed NH4Cl and ascorbic acid treatment can decrease the urine pH at 8 cats (89%) with an average pH 6.3 ± 0.3. Microscopic examination of the urine showed the struvite crystallization more infrequently than before therapy. The study concluded that the combination of NH4Cl and asam askorbat can lower the urine pH and reduce struvite crystal density in the urine.
Infeksi Microsporum canis pada Kucing Penderita Dermatitis (MICROSPORUM CANIS INFECTION IN DERMATITIS CATS) Soedarmanto Indarjulianto; Yanuartono Yanuartono; Sitarina Widyarini; Slamet Raharjo; Hary Purnamaningsih; Alfarisa Nururrozi; Nurman Haribowo; Hizriah Alief Jainudin
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.373 KB) | DOI: 10.19087/jveteriner.2017.18.2.207

Abstract

Dermatitis in cats can be caused by Microsporum canis and is zoonotic. This study aims to perform clinical examination, laboratory examination, isolation, and identification of mold M. canis in cats with dermatitis. Skin scrapped from 30 cats that clinically showed lesions of dermatitis i.e. combination of alopecia, erythema, papules, pustules, scaly, and crusty were used in this study. Examination of clinical lesions and screening tests using the Wood’s lamps were performed prior to sampling. Skin scrapes samples were cultured onto Sabouraud’s dextrose agar medium, incubated at 28 ° C and colonies were identified macroscopically then stained using Lactophenol Cotton Blue for microscopic examination. Seventen of the 30 samples (56.7%) were identified as M. canis macroscopically and microscopically. The skin lesions observed in the 17 M. canis infected cats were erythema, alopecia, scaly, and crusty distributed to the ear, body, neck, back and tail of cats, respectively ABSTRAK Dermatitis pada kucing dapat disebabkan oleh Microsporum canis dan bersifat zoonotik. Penelitian ini bertujuan melakukan pemeriksaan klinis, pemeriksaan laboratoris, isolasi, dan identifikasi kapang M. canis pada kucing penderita dermatitis. Kerokan kulit dari 30 ekor kucing yang secara klinis menunjukkan lesi dermatitis berupa kombinasi dari alopesia, eritema, papula, pustula, bersisik, dan berkerak digunakan dalam penelitian ini. Pemeriksaan lesi klinis dan uji screening dengan lampu Wood’s dilakukan sebelum pengambilan sampel. Sampel kerokan kulit dikultur pada media Sabouraud’s dextrose agar, diinkubasi pada suhu 28°C dan diidentifikasi secara makroskopis. Koloni yang tumbuh diperiksa secara mikroskopis menggunakan pewarnaan Lactophenol Cotton Blue. Hasil penelitian menunjukkan 17 dari 30 sampel (56,7%) teridentifikasi M. canis secara makroskopis dan mikroskopis. Lesi kulit yang ditemukan pada 17 kucing positif terinfeksi M. canis menunjukkan adanya eritema, alopesia, bersisik, dan berkerak dengan lokasi penyebaran pada telinga, badan, leher, punggung atau ekor.
Deteksi Bovine Herpesvirus-1 Secara Immunohistokimia pada Membran Korioallantois Telur Ayam Berembrio (IMMUNOHISTOCHEMISTRY DETECTION OF BOVINE HERPESVIRUS-1 IN CORIOALLANTOIC MEMBRANE OF CHICKEN EMBRYONATED EGG) Yuli Purwandari Kristianingrum; Charles Rangga Tabbu; Bambang Sutrisno; Sitarina Widyarini; Kurniasih .; Tri Untari; Asmarani Kusumawati
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Infectious Bovine Rhinotracheitis (IBR) is caused by Bovine Herpes virus-1 in the cattle. The clinicalsigns demonstrate depression, anorexia, swelling of the vulva, redness of the vestibule, pustule and ulceron the vaginal mucosal. Based on previous research, IBR virus from the nasal swab could be grown inchorio-allantoic membrane of embryonated chicken eggs. This study aim was to confirm whether IBR virusin cattle could be grown in embryonated chicken eggs as a substitute for cell culture. A total of five nasalswab samples from the cows that were positive for IBR infection (diagnosed by Polymerase Chain Reactionand cell culture) were inoculated on the chorio-allantois membrane of embryonated chicken eggs.Observation of lesions performed at 3-5 days after inoculation. Re-inoculation (passage) was done threetimes. Pock characteristic lesions were observed on the corioallantoic membrane with the size of 5-7 mm,rounded shape, opaque edge, with necrosis in the central area. Furthermore, pock lesions were processedfor hematoxylin and eosin staining and immuno-histochemistry. The result of hematoxylin and eosinstaining showed that the formation of intranuclear inclusion bodies and vacuolization of the epithelial cellof membrane was observed. Immuno-histochemistry staining showed positive reaction for antibodiesagainst BHV-1 in the epithelial cells membrane. In conclusion, embryonated chicken eggs could be usedas a medium for detection of IBR.
Kepadatan Sel Hipokampus Insulin Imunoreaktif pada Formasi Hipokampus Mencit yang Diinduksi Berulang dengan Streptozotosin (THE DENSITY OF HIPPOCAMPUS INSULIN IMMUNOREACTIVE CELLS IN HIPPOCAMPUS FORMATION OF REPEAT STREPTOZOSIN INDUCED MICE) Erwin .; Tri Wahyu Pangestiningsih; Sitarina Widyarini
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The presence of insulin in the hippocampus may indicate its involvement in brain cognitive function,such as learning and memory phenomena. The purpose of this study was to find out the density ofhippocampus insulin immunoreactive cells in hippocampus formation in Balb-C mice which treated withstreptozosin repeated as the animal model of diabetes mellitus. Thirty male mice Balb-C strain, aged 12-14 weeks, weight 30-40 g, divided into 2 treatment groups, each group consisted of 15 individuals. GroupI (KI) was treated with sodium citrate buffer, while group II (K2) was treated with streptozotosin at  dose0,5 ml of 40 mg/kg bw in 50 mM sodium citrate buffer pH 4.5 in intra-peritoneal of for five consecutive days.Every two animals from each group euthanasia and necropsied on day 7, 14, 21 and 28 respectively afterthe administration of treatment. Subsequently, the brain tissues were collected and fixatived in NBF10%. Brain sampel were the processed immunohistochemically using anti-insulin mouse antibody. Thedensity of hippocampus insulin immunoreactive cells in hippocampus formation in group 1 were highercompared to group 2. This comparasion as well as the time of observation and interaction between groupand time showed significant differences (p<0.05). it can be concluded that low-dose induction of repeatedstreptozotosin may  cause a decrease in density of hippocampus insulin imunoreaktif cell.
Jumlah dan Ukuran Nodul Tumor Kolorektal Berbanding Lurus dengan Ekspresi Siklooksigenase-2 (THE CORRELATION OF THE NUMBER AND THE SIZE OF COLORECTAL TUMOR NODULE WITH CYCLOOXYGENASE-2 EXPRESSION) Risfah Yulianty; Riska Nufika; Sitarina Widyarini
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Study chemically induced colon cancer with 1,2-Dimethylhydrazine (DMH) has been widely used.DMH induction to rodentia as animal model have been similarity with colon cancer morphlogy of human.Cyclooxygenase-2 (COX-2) are important enzymes that affect carcinogenesis.COX-2can be induced byvarious stimuli, including inûammation, growth factors, and cytokines produced by tumor cells. The aim ofthis study is  to investigate the correlation between the expression  of COX-2 and the doses variation ofDMH, the number and the size of colorectal tumor nodule.Six male Wistar rats were randomly divided into 4 groups each group consists of six rats.  Group I is control group.  Group II, III, and IV were given 20, 40,and 60 mg/kg BW of DMH respectively.  DMH  was subcutaneously injected in the hip area, once a week for15 weeks.  All animals were sacrificed at week 26th, the colon were removed and fixed in 10% bufferformalin for macroscopic and microscopic examination.  The results of this study showed that there is acorrelation between various doses of DMH  (P<0.05) with number of tumor nodule but not with the size ofcolorectal tumor nodule (P>0.05). Microscopic examination by using HE staining shows that inductionwith doses variation ofDMHresultin different morphololical form of tumour tissue.  Induction of 20, 40,and 60 mg/kg BW DMH result in adenoma(100.00%),adenoma and adenocarcinoma (33.30% and 66.67%),and 100.00% adenocarcinoma respectively.  The happens of COX-2 also correlate with in this studythatexpression of COX-2 correlate with the number and size of colorectal tumor nodule (P<0.05). However,there is no correlation between COX-2 expressionand doses variation of DMH.  In conclusion, there is adose-dependent response in the number and the size of colorectal tumor nodule.
Dampak Induksi Karsinogenesis Glandula Mammae dengan 7, 12-dimetilbenz(?)antrasen terhadap Gambaran Histopatologis Lambung Tikus Sprague Dawley Ranita Tri Budi M; Sitarina Widyarini
Jurnal Veteriner Vol 11 No 1 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This experiment was designed to study the impact of mammary carcinogenesis induced by 7,12-dimetilbenz (?) antracene (DMBA) on histopathological features of the gastric in Sprague Dawley rat.Eight female Sprague Dawley (SD) rats, of four week-old, were used in this study. The rats were randomlydivided into 2 groups of four. Group I were treated orally with 20 mg/kg BW of 7,12-dimetilbenz (?)antracene (DMBA) treatment, twice a week for 5 weeks. Group II were used as negative control withoutDMBA treatment. At 5 week after the end DMBA of, the rats of the both groups were sacrificed and thegastric were fixed in 10% buffer formalin. Subsequently, tissue sections were stained with hematoxylinand eosin (H&E) for microscopic examination. The results of the experiment show that oral administrationof DMBA (20 mg/kg BW) twice a week, for 5 weeks result in epithelial hyperplasia of cardia. Hemorrhagicand inflammation reactions are also found on the mucous membrane of fundic. However, macroscopicexamination shows no specific changes of the gastric. Histopathological changes were suspected as a precancer lesion of the gastric due to chronic exposure of DMBA.
The relationship between sirtuin 1 (SIRT1) expression and tumor size, Proliferating Cell Nuclear Antigen (PCNA) expression and histological grading in rat breast carcinoma induced by dimethylbenz()anthracene (DMBA) Novrita Padauleng; Dewajani Purnomosari; Sri Herwiyanti; . Harjadi; . Irianiwati; Sitarina Widyarini
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 04 (2013)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2060.833 KB) | DOI: 10.19106/JMedScie004504201301

Abstract

Controversy regarding the role of SIRT1 in pathology of cancers exists and is still under debate.SIRT1 could act as either a tumor supressor or tumor promotor. This study was conducted toevaluate the relationship between SIRT1 expression and tumor size, Proliferating Cell NuclearAntigen (PCNA) expression and histological grading in rat breast carcinoma induced bydimethylbenz(á)anthracene (DMBA). Thirty female Sprague Dawley rats were randomly allocatedinto three groups with 10 rats in each group. Group 1 as negative control was just fed thestandard food. Group 2 as vehicle control was fed the standard food and corn oil. Group 3 asinduction group was fed the standard food and induced with DMBA at dose of 20 mg/kg bodyweight (BW) in corn oil twice a week for five weeks. All rats were palpated weekly to determinethe appearance, size and location of tumors. Sixteen weeks after DMBA induction rats weresacrified and histological preparations of the breast carcinoma tissue were then processed forSIRT1 and PCNA expression examination as well as histological grading. The result showed thatSIRT1 expression was significantly higher in breast carcinoma tissue compared to normal gland(26.12 vs 0.05; p = 0.004). SIRT1-positive was observed mostly in poor histological gradecarcinomas (56.2%), and it was not observed in good histological grade carcinomas. However,there was no significantly difference between SIRT1 and histological grading (p = 0.097; r =0.285). A significant correlation between SIRT1 expression and the tumor size (p =0.009; r=0.877), as well as PCNA expression (p =0.000; r =0.790) was observed. In conclusion, thereis relationship between SIRT1 expression and tumor size as well as PCNA expression in rat breastcarcinoma induced by DMBA.
PENGARUH PEMBERIAN KOMBINASI FRAKSI ETIL ASETAT AKAR PASAK BUMI DAN DOXORUBICIN TERHADAP BERAT BADAN DAN JUMLAN NODUL TIKUS SPRAGUE DAWLEY BETINA YANG DIINDUKSI 7,12 DIMETILBENZ(α) ANTRASEN (DMBA) Laela Hayu Nurani; Achmad Mursyidi; Sitarina Widyarini
Pharmaciana Vol 6, No 1 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.079 KB) | DOI: 10.12928/pharmaciana.v6i1.3093

Abstract

Pasak Bumi roots contain quasinoid compounds that have carcinogenesis inhibitory, imunomudulator, antiulcer, and antimalaria effects. This study aims to determine the effect of a combination of ethyl acetate fraction of Pasak Bumi roots and doxorubicin on DMBA-induced white mice by weight and palpation observation. The mice were divided into 8 groups. Each group consisted of 7 female Sprague Dawley mice. Group 1 (normal) was only given food and drink, Group 2 was given fraction of ethyl acetate of Pasak Bumi roots (100 mg/kg body weight, Group 3 (positive control) was given doxorubicin (1.17 mg/kg body weight) 1 times a week for 5 weeks, Group 4 was given DMBA (20 mg/kg) two times a week for 5 weeks, Groups 5 was given DMBA (20 mg/kg) + doxorubicin (1.17 mg/kg), Group 6 was given DMBA (20 mg / kg) + ethyl acetate fractions of Pasak Bumi roots (100 mg / kg), Group 7 was given DMBA (20 mg / kg) + doxorubicin (1.17 mg / kg) + ethyl acetate fraction of Pasak Bumi roots (100 mg / kg) and Group 8 doxorubicin ( 1.17 mg / kg body weight) + ethyl acetate fraction of Pasak Bumi roots (100 mg / kg). Observations were made on the basis of body weight of mice and tumor incidence by palpation at week 6 of treatment. The result of  this research shows that the administration of ethyl acetate fraction of Pasak Bumi roots and doxorubicin combination can increase body weight of the mice and decrease the number of tumors compared to single doxorubicin.
EFEK EKSTRAK ETANOL GANGGANG HIJAU (Ulva lactuca , L) TERHADAP EKSPRESI COX-2 MIOKARDIUM TIKUS YANG DIINDUKSI ISOPROTERENOL Wahyu Widyaningsih; Nina Salamah; Suwidjiyo Pramono; Sugiyanto Sugiyanto; Sitarina Widyarini
Media Farmasi: Jurnal Ilmu Farmasi Vol 16, No 2: September 2019
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.479 KB) | DOI: 10.12928/mf.v16i2.12822

Abstract

Penyakit kardiovaskuler masih merupakan masalah di Indonesia salah satunya adalah Infark Miokardial Akut (IMA). Patofisiologis AMI karena rective oxigen spesies  (ROS) melibatkan stimulasi sitokin pro inflamasi salah satunya adalah  interleukin-1 yang berakibat peningkatan ekspresi COX-2. Penelitian ini bertujuan mengetahui efek ekstrak etanol ganggang hijau dalam menurunkan ekspresi COX-2 pada tikus IMA yang diinduksi isoproterenol (ISO). Ekstraksi ganggang hijau dengan maserasi menggunakan etanol 96%. Tikus berat 200-250 g dibagi menjadi 6 kelompok masing-masing 6 ekor. Kelompok I kontrol, tikus tidak diberi ISO hanya diberi CMC Na, kelompok II kelompok ISO, tikus diberi CMC Na dan ISO, kelompok III, IV dan V tikus diberi ekstrak etanol ganggang hijau dosis 250, 500 dan 750 mg/KgBB peroral dan kelompok VI diberi melatonin 10 mg/KgBB. Perlakuan ekstrak selama 28 hari. Pada hari ke 29 dan 30 tikus diberi isoproterenol dosis 85 mg/KgBB selama 2 kali pemberian secara subkutan kecuali kelompok I. Pada hari ke 31 tikus diambil organ jantung kemudian dibuat preparat histologis dan dilakukan analisis ekspresi COX-2 dengan metode imunohistokimia dengan antibodi anti COX-2. Hasil penelitian menunjukkan bahwa pemberian ekstrak etanol ganggang hijau dosis 250, 500 dan 750 mg/KgBB dapat menurunkan ekspresi COX-2 pada tikus yang diinduksi isoproterenol.
UJI EFEK HAMBATAN ATORVASTATIN TERHADAP PEMBENTUKAN SKAR HIPERTROFI PADA KELINCI NEW ZEALAND (Kajian terhadap morfologi klinis ketinggian jaringan skar, Scar Elevation Index, kepadatan kolagen, dan ekspresi VEGF) Devi Usdiana Rosyidah; Indwiani Astuti; Sitarina Widyarini
Biomedika Vol 10, No 1 (2018): Biomedika Februari 2018
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v10i1.5848

Abstract

Skar hipertrofi dan keloid bagian dari jaringan parut patologis yang insiden kejadiannya masih tinggi. Keduanya mengganggu secara estetik, fisiologik, psikologik, dan sering tumbuh kembali setelah pengobatan. Belum ada terapi standar baku emas untuk skar hipertrofi dan keloid. Efek pleiotropik statin berpotensi sebagai anti skar baru. Penelitian ini bertujuan untuk mengetahui efek hambatan pembentukan skar hipertrofi dengan pemberian salep atorvastatin pada model luka skar di telinga kelinci New Zealand. Kelinci New Zealand usia 4-6 bulan (n=29) dilakukan randomisasi kedalam lima kelompok. Setelah aklimatisasi 1 minggu, dilakukan pembuatan luka model skar hipertrofi pada kedua telinga kelinci dengan punch biopsy (d=8 mm). Jumlah luka 2 buah di masing- masing telinga kanan dan kiri. Pada hari ke-7 setelah punch biopsy, kelompok I, II, III dioles salep atorvastatin dengan konsentrasi masing-masing 5%, 10%, dan 20%. Kelompok IV dioles salep clobetasol propionate 0,05% sebagai kontrol positif, dan kelompok V dioles dengan basis salep (lanolin vaselin 1:1) sebagai kontrol negatif. Frekuensi pengolesan salep pada luka ditelinga kelinci tersebut 1 kali/hari, dengan lama pengolesan selama 50 hari kedepan. Setiap hari dilakukan pengukuran morfologi klinis ketinggian skar. Pada hari ke 56 setelah punch biopsy, dilakukan penilaian akhir morfologi klinis jaringan skar hipertrofi, kemudian kelinci didekapitasi, diambil jaringan skar hipertrofi kemudian dibuat preparat histologis (HE, Von Gieson) dan imunohistokimia (VEGF). Salep atorvastatin 5% mampu menghambat pembentukan skar hipertrofi pada telinga kelinci New Zealand dengan nilai median skoring ketinggian jaringan skar sebesar 1(1-3), nilai SEI rata-rata sebesar 1,49±0,37 mm, dan nilai ekspresi VEGF sebesar 12,71±4,27%. Salep atorvastatin 5% mempunyai potensi untuk dikembangkan sebagai obat penghambat pembentukan skar hipertrofi baru dengan mekanisme penghambatan terhadap VEGF. Untuk pengembangan obat ini diperlukan penelitian lebih lanjut mengenai kapan waktu yang paling tepat mulai dioleskan salep atorvastatin setelah terjadi luka berisiko terbentuknya skar hipertrofi. Kata kunci: Atorvastatin, skar hipertrofi, VEGF, telinga kelinci.
Co-Authors . Harjadi . Muttaqien Abd. Rasyid Syamsuri Abdul Rohman Achmad Mursyidi Achmad Mursyidi Adrenalin, Sruti Listra Agnes Nora Iska Harnita Agnesia Endang Tri Hastuti Wahyuni Agung Endro Nugroho Agung Giri Samudra Agustina Dwi Wijayanti AGUSTINUS YUSWANTO Ahmad Mursyidi Ahmadi, Maulidina Alfarisa Nurrurozi Alfarisa Nururrozi Alfarisa Nururrozi Alfarisa Nururrozi Alfarisa Nururrozi Ana Sahara Anandita Nurwijayanti Andayana Puspitasari Gani Anna Ekawati Apriani, Lalily Arief Nurrochmad Asmarani Kusumawati Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu Claude Mona Airin Deasy Wulan Dwiratna Devi Usdiana Rosyidah Devita Anggraeni Dewajani Purnomosari Dewajani Purnomosari Dhasia Ramandani, Dhasia Dhirgo Adji Dimas Adhi Pradana Dwi Priyowidodo Edy Meiyanto Eka Kumalasari Endang Dwi Wulansari Erma Yunita Erwin . Erwin E Eryl Sri Rohayati Eti Nurwening Sholikhah Etriwati E Fajar Slamet Riana Fara Azzahra Fara Azzahra FARIDA HAYATI Fauziah, Ima Frans J.M. Harren Galih Dwi Mulyati Gede Bayu Suparta Gemini Alam Geovani Meryza Oka Putra Caesar Gharsina Ghaisani Yumni Hady Anshory Hardian, Andreas Bandang Harjadi Harjadi Hary Purnamaningsih Hary Purnamaningsih Hastari Wuryastuty Hayati, Farida Helmina Wati Hizriah Alief Jainudin Ign Edi Santosa Ika Tidariani Imanjati, Lynda Nugrahaning Indwiani Astuti Indwiani Astuti Irianiwati Irianiwati Irianiwati Widodo Iwan Sahrial Hamid Joko Prastowo Jumina Jumina Kintoko, Kintoko Kony Putriani Kurniasih . Kurniasih Kurniasih Kurniasih Kurniasih Laela Hayu Nurani Laela Hayu Nurani Laela Hayu Nurani Laela Hayu Nurani Laksono Trisnantoro Lalily Apriani Laras Widawaty Putri Lintang Cahya Saputri Lukman Hakim Lukman Hakim Marchaban Marchaban Mega Cahya Nalasukma Michael Haryadi Wibowo Mustofa Mustofa Mustofa Mustofa Nanang Fakhrudin, Nanang Nemay Anggadewi Ndaong Neni Tri Anggraini Ngatidjan Ngatidjan Ni'ma, Neli Syahida Nina Salamah Noor Ardhi Pratomo Novrita Padauleng Novrita Padauleng Nugrahani, Warih Pulung Nura Ustrina Nurman Haribowo Pramono, Suwidjiyo Puspa Wikan Sari R Wasito R Wasito Rahmawati, Irhamna Putri Ramadhani, Mungky Ema Ranita Tri Budi M Rifqi Ferry Balfas Rini Widayanti Risfah Yulianty Risfah Yulianty Riska Nufika Riska Nufika Ruslin Hadanu Rusmihayati Rusmihayati Santika, Luh Putu Nadya Santoso, Ferdinand Prayogo Cahyo Sardjiman S Siti Salma Yusuf Slamet Raharjo Slamet Raharjo Soedarmanto Indarjulianto Soedarmanto Indarjulianto Soedarmanto Indarjulianto Sri Herwiyanti Sri Herwiyanti, Sri Subagus Wahyuono Subagus Wahyuono Sudarsono Sudarsono Sudibyo Martono Sugiyanto - Sugiyanto . Sugiyanto Sugiyanto Sugiyanto Sugiyanto Sugiyanto, Sugiyanto Sugiyono Sugiyono Sugiyono Sugiyono Sugiyono Sugiyono Sugiyono Sultan Suhrah Febrina Karim Suwidjiyo Pramono Suwidjiyo Pramono Suwidjiyo Pramono Suwidjiyo Pramono Tiaravista, Amanda Gita Tri Untari Tri Untari Tri Untari Tri Wahyu Pangestiningsih Tria Zakinah Vembriarto Jati Pramono Vinsa Cantya Prakasita Wahyu Widiyaningsih Wahyu Widyaningsih Wahyu Widyaningsih Widhihastuti, Endah Winarsih, Sugi Yanuartono . Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono yanuartono, yanuartono - Yeremia Yobelino Sitompul Yuli Purwandari Kristiangingrum Yuli Purwandari Kristianingrum Yuli Purwandari Kristianingrum, Yuli Purwandari Yunitasari, Maria Yustina Sri Hartini Yusuf, Siti Salma