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Capsule Formulation of Ethanolic Extract of Pasak Bumi (Eurycoma Longifolia Jack.,) and its Effect on Human Health Vital Signs Laela Hayu Nurani; Eka Kumalasari; Abdul Rohman; Sitarina Widyarini
Majalah Obat Tradisional Vol 22, No 2 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.131 KB) | DOI: 10.22146/tradmedj.27919

Abstract

Pasak bumi (Eurycoma longifolia) has the potential to be developed as antihypertensive, antipyretic, aphrodisiacs and health supplements. The use of E. longifolia as a traditional medicine needs to be pursued in the form of more effective and appropriate formulation. The capsule preparations are easy to make and cancover the bitter taste of E. longifolia. Clinical trials in this study use design pre-post treatment in healthy humans. Subjects used were male - healthy men and healthy women who met inclusion criteria and were subjected with formulated capsule for 14 days. The study resulted capsule formula comprising of the ethanolic extract of E. longifolia 300 mg, vivapur 101 300 mg, 58 mg maydis starch, aerosil 3%, talc 2%, and Mg stearate 1%. The results showed that the capsule of E. longifolia did not affect the value of heart rate, respiration rate, body temperature and weight (P > 0.05), based on paired t-test, but they causes a decrease in blood pressure of healthy human. The ethanol extract of E. longifolia caused vasodilation of blood vessels that can be used in antihypertensive therapy.
Radical Scavenging Activity Combination of Sambiloto (Andrographis paniculata Nees.) and Patikan Kebo (Euphorbia hirta L.) Ethanolic Extracts on 2,2-Diphenyl-1-Picrylhidrazyl (DPPH) Andayana Puspitasari Gani; Suwidjiyo Pramono; Sudibyo Martono; Sitarina Widyarini
Majalah Obat Tradisional Vol 23, No 3 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.548 KB) | DOI: 10.22146/mot.31600

Abstract

Research of radical scavenging activity of sambiloto (Andrographis paniculata Nees) and patikan kebo (Euphorbia hirta L) ethanolic extracts and their combination toward 2,2-Diphenyl-1-Picrylhidrazyl (DPPH) has conducted. Subjects of this experiment comprise sambiloto ethanolic extract, patikan kebo ethanolic extract, dechlorophyllated extract of sambiloto and combinations among those three. Dechlorophyllation by electrocoagulation method is done to reduce chlorophyll content thereby increasing the level of active compound in the sambiloto extract. The results of dechlorophyllation showed that only total flavonoid content in the extract of sambiloto increased, meanwhile the radical scavenging activity results showed that patikan kebo ethanolic extract has the lowest IC50 value (22,36 µg/mL) compared to sambiloto ethanolic extract (IC50 value 499,03 µg/mL). Extract combination may increase DPPH radical scavenging activity of the sambiloto extract (IC50 value 76,06 µg/mL) but not higher than that of patikan kebo single extract.
Topical Anti-inflammatory Activity of Bangle (Zingiber cassumunar Roxb). Ethanolic Extract in Mice Induced By Carrageenan Endang Dwi Wulansari; Subagus Wahyuono; Marchaban Marchaban; Sitarina Widyarini
Majalah Obat Tradisional Vol 23, No 2 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (760.451 KB) | DOI: 10.22146/mot.34640

Abstract

Rhizome of bangle (Zingiber cassumunar Roxb.) is one of the materials that are traditionally used in the traditional preparation to maintain skin from redness. The content of chemical compound in bangle rhizomes has many benefits, including inhibition of the inflammatory processes. This study aims to determine the topical anti-inflammatory activity of ethanolic extract of bangle rhizomes in mice induced by carrageenan. Thirty five female Swiss mice were used in this study. Animals were divided into 7 group of five: normal group, carrageenan 2% group, carrageenan 2% group plus control of basis (Biocream®), carrageenan 2% group plus 2.5% hydrocortisone cream, and carrageenan 2% group plus cream of ethanolic extract of bangle rhizomes with the concentration of 1%, 2.5% and 5% w/w. Carrageenan 2% was injected subcutaneously in all treatment group except normal group. The skinfold thickness of mice was measured every 1 hour for 6 hours after carrageenan injection. Results of this study shows that there is a significant differences between treatment groups (p <0.05) compared to carrageenan 2% treated group. To conclude, ethanolic extract of bangle rhizomes has topical anti-inflammatory in mice induced by carrageenan. 
The Effect of Pasak Bumi (Eurycoma longifolia, Jack) Roots Ethanol Extract against Hematology Profile of Healthy Volunteers Laela Hayu Nurani; Fara Azzahra; Sitarina Widyarini; Abdul Rohman
Majalah Obat Tradisional Vol 24, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.45533

Abstract

The roots of Eurycoma longifolia, Jack has a lot of beneficial activity on human health. The use of Eurycoma longifolia, Jack roots as a traditional medicine should be made in the form of a dosage that is more effective, safe, and had no side effects, especially on hematology. Easy preparations are capsule. This study aimed at identifying the effect of ethanol extract capsule of Eurycoma longifolia, Jack roots on hematology of healthy volunteers. Healthy volunteers were assigned in the study, each of which were 10 male and 10 female who met the inclusion criteria. Both groups were given ethanol extract capsule of Eurycoma longifolia, Jack roots for 14 days, with the dose of 300 mg extract once a day, after meal, at night. Hematology examination was performed on day 0; 14 and 42. Results were compared statistically using Repeated ANOVA and Friedman tests. The result of the study indicated that the average value of hematology at the examination day of 0; 14; and 42, there were significant differences in MCV and leukocyte parameters in healthy male volunteers (p<0.05). MCH, LED and monocyte parameters in healthy female volunteers had a significant difference of (p<0.05). However, the parameter values were still within the normal range. Monitoring results showed that some of the side effects were frequent urinary, dizziness, nausea, sweating, insomnia, and increasing appetite on healthy male volunteers. Meanwhile, on healthy female volunteers, there were frequent urinary, insomnia, constipation. Having ethanol extract capsule of the Eurycoma longifolia, Jack roots did not affect the hematology parameters of healthy male and female volunteers, but it caused some side effects. 
CYP1A1 and GSTm expression of hepatocytes induced by 7,12-dimethylbenz(a)anthracene and the influence of ethanolic extract of Gynura procumbens Iwan Sahrial Hamid; Sugiyanto .; Edy Meiyanto; Sitarina Widyarini
Indonesian Journal of Pharmacy Vol 20 No 4, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.093 KB) | DOI: 10.14499/indonesianjpharm0iss0pp198-206

Abstract

The cytochrome P-450 (CYP) and glutathione S-transferase (GST) enzyme systems may influence the biological effects of carcinogens. As such, these enzymes may predict the developmental risk of breast cancer, as well as be potential targets for chemoprevention. The purpose of this study was to compare the expression of CYP1A1 and GST* between treatment groups. Expression of CYP1A1 and GST* was quantified in liver tissue from 18 female Sprague Dawley rats aged 40 days, which randomly divided into six treatment groups. Those are base line group (without DMBA and ethanolic extract treatment), DMBA induced cancer group, the other two groups was administrated by DMBA after treated by ethanolic extract in two different doses, 300 mg /kg BW and 750 mg/kg BW. The last two groups were given two doses of extract ethanolic only, without initiated of DMBA. The ingestion of the extract was carried for three weeks and the ingestion of DMBA was performed twice in the third week. The expression CYP1A1 and GST was quantified by immunohistochemistry. CYP1A1 expression was significantly higher (P < 0.05) in cancer group (DMBA) as compared with other groups. On the other hand, GST expression was lower in cancer group (P < 0.05). Result of this study demonstrated that ethanolic extract leaves of G. procumbens in 300 mg/kg BW dose could inhibit CYP1A1 stronger than are other and induced GST* level. Has effect on ethanolic extract leaves of G. procumbens has ability in played role of as blocking agent in preventing initiation stage of carsinogenesis, therefore it should be taken into account for chemopreventing agent in mammary carsinogenesis.Key words : Gynura procumbens, breast cancer, Chemopreventive, CYP1A1, GSTμ
KAJIAN ETNOBOTANI, FITOKIMIA, FARMAKOLOGI DAN TOKSIKOLOGI SUKUN (Artocarpus altilis (Park.) Fosberg) Gharsina Ghaisani Yumni; Sitarina Widyarini; Nanang Fakhrudin
Jurnal Tumbuhan Obat Indonesia Vol 14 No 1 (2021): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jtoi.v14i1.3944

Abstract

ABSTRACT Indonesia is a country with large plant biodiversity with medicinal properties, such as “sukun” (Artocarpus altilis) or known as “breadfruit”. Breadfruit is a woody evergreen plant that has been used traditionally for various purposes, including medication. The fruit is rich in carbohydrates and fibers as a food source. The leaf and cortex are the most widely used for treating various diseases and other health benefits. This article aimed to present a comprehensive review on the potency of breadfruit from the perspective of ethnobotany, phytochemistry, pharmacology, and toxicology. The data in this narrative review was obtained from the scientific journals in the databases of Google Scholar, PubMed, Scopus, and ScienceDirect. Other credible sources, such as textbooks, student thesis, and patents were also used to support the main data. Based on the literature study, breadfruit has been used empirically in Indonesia as a medicinal herb. The scientific data of breadfruit showed antiinflammatory, antiplatelet, antioxidant, antiatherosclerosis, antihyperlipidemic, antimalaria, antidiabetic, cardioprotective, and anticancer activities. Breadfruit contains terpenoids, flavonoids, alkaloids, and phenolics as bioactive compounds. However, the unique compounds are geranylated and prenylated flavonoids such as cycloartenol, artonin V, and cyclomulberin. These compounds are distributed in the leaf, cortex, wood, and fruit. Limited data is available regarding the toxicology profile of breadfruit. Breadfruit leaves ethanol extract did not show any significant toxic effects in the animal experiments. However, the toxicity of the water extract is unclear, and thus, needs to be investigated to ensure its safety. Keywords: Artocarpus communis, bioactivity, chemical constituents, ethnopharmacology ABSTRAK Indonesia dikenal sebagai negara yang kaya akan keragaman tumbuhan berpotensi obat, diantaranya sukun (Artocarpus altilis). Sukun merupakan tanaman berkayu yang secara tradisional dimanfaatkan untuk berbagai keperluan termasuk pengobatan. Buah sukun mengandung karbohidrat dan serat sebagai sumber pangan. Daun dan batang sukun merupakan bagian yang paling banyak dimanfaatkan dalam pengobatan dan kesehatan. Reviu artikel ini bertujuan untuk mengkaji secara komprehensif potensi sukun dari sudut pandang etnobotani, fitokimia, farmakologi, dan toksikologi. Artikel narrative review ini ditulis berdasarkan data yang diperoleh dari kajian literatur hasil penelitian yang ada di basis data Google Scholar, PubMed, Scopus, dan ScienceDirect. Beberapa sumber pustaka lain seperti buku, naskah tugas akhir dan paten juga digunakan untuk memperkaya penulisan. Hasil kajian literatur sukun menunjukkan bahwa tanaman ini memiliki riwayat empiris digunakan sebagai obat tradisional di Indonesia. Hasil penelitian ilmiah menunjukkan sukun memiliki aktivitas antiinflamasi, antiplatelet, antioksidan, antiatherosklerosis, antihiperlipi-demia, antimalaria, antidiabetes, kardioprotektif, dan antikanker. Sukun mengandung senyawa terpenoid, flavonoid, alkaloid, dan senyawa fenolik. Senyawa khas dari tumbuhan genus Artocarpus ini adalah flavonoid dengan gugus geranil atau prenil, misalnya sikloartenol, artonin V, dan siklomulberin. Senyawa tersebut tersebar dalam daun, kulit kayu, batang, dan buah. Data terkait profil toksikologi sukun masih terbatas. Ekstrak etanol daun sukun tidak menunjukkan efek toksik pada hewan uji. Namun, ekstrak airnya belum memiliki profil toksikologi yang jelas sehingga perlu dilakukan pengujian untuk memastikan keamanannya. Kata kunci: Artocarpus communis, bioaktivitas, kandungan kimia, etnofarmakologi
EFEK KO-KEMOTERAPI FRAKSI ETIL ASETAT AKAR PASAK BUMI DAN DOXORUBICIN TERHADAP PROLIFERASI DAN EKPRESI BAX JARINGAN PAYUDARA TIKUS SD Tria Zakinah; Laela Hayu Nurani; Sitarina Widyarini
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 14, No 1 (2017)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (741.051 KB) | DOI: 10.24071/jpsc.00559

Abstract

The use of co-chemotherapy agent was needed since there were some toxicities on the normal tissues caused by the use of doxorubicin. The root of Eurycoma longifolia Jack, a part of plant, has a potential activity as co-chemotherapy. This study aimed to determine the effect of co-chemotherapy of ethyl acetate fraction of E. longifolia Jack roots and doxorubicin against cell proliferation activity by AgNOR method and determine expression of Bax protein of breast tissue in rats induced by DMBA. The rats were divided into five groups: I (baseline), II (DMBA 20 mg/kgBW), III (DMBA, doxorubicin 1.12 mg/kg), IV (DMBA, fraction of 100 mg/kg), and V (DMBA, doxorubicin, fractions). All the rats were sacrificed at week 16. Their breast tissue was evacuated. Cell proliferation and expression was observed using AgNOR method and immunohistochemistry, respectively. Results showed the percentage of p-AgNOR obtained by the healthy group, DMBA group, DMBA+Doxorubicin group, DMBA+ethyl acetate fraction of E. longifolia Jack root group, and DMBA+Doxorubicin+Ethyl acetate fraction of E. longifolia group were 00%, 14.672.11%, 1.831.21%, 6.832.03%, and 4.080.95%, respectively. The percentage of Bax expression obtained by the healthy group, DMBA group, DMBA+Doxorubicin group, DMBA+ethyl acetate fraction of E. longifolia Jack root group, and DMBA+Doxorubicin+Ethyl acetate fraction of E. longifolia group were 68.821.52%, 26.863.25%, 44.492.06%, 80.923.27%, and 78.704.87%, respectively. Based on the results, it was concluded that co-chemotherapy agent of ethyl acetate fraction of E. longifolia Jack roots and Doxorubicin could inhibit proliferation and trigger apoptosis through Bax expression in breast tissue of rats induced by DMBA.
Uji Aktivitas Fagositosis Makrofag Fraksi-fraksi dari Ekstrak Metanol Daun Sirih Merah (Piper crocatum Ruiz & Pav.) Secara In Vitro YUSTINA SRI HARTINI; SUBAGUS WAHYUONO; SITARINA WIDYARINI; AGUSTINUS YUSWANTO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (654.615 KB)

Abstract

Plants can be sources of immunomodulatory agents. Piper crocatum Ruiz & Pav. (red betel) leaves have been used to heal many diseases, especially to increase endurance. The aim of this research was to investigate the imunomodulatory effect of methanolic leaf extracts of Piper crocatum Ruiz & Pav. and its fractions by in vitro phagocytic macrophage activity test. The result showed that methanolic extract of Piper crocatum Ruiz & Pav. 75μg/mL could stimulate phagocytic macrophage activity equal to 100μg/mL product-X® that contained Echinacea extract. Separation of the extract by vacuum liquid chromatography using gradient solvent n-hexane - ethyl acetate yielded 5 fractions. The second fraction showed the highest activity. It contained alkaloid and terpenoid, while the extract also contained flavonoid and essential oil. Alkaloid and/or terpenoid could be the compound in the leaves of Piper crocatum Ruiz & Pav. that responsible increasing phagocytic macrophage activity.
Pengaruh Pemberian Ekstrak Akar Pasak Bumi Terstandar terhadap Gambaran Histopatologik Testis dan Konsentrasi Testosteron pada Tikus FARIDA HAYATI; SITARINA WIDYARINI; LUKMAN HAKIM; NGATIDJAN NGATIDJAN; MUSTOFA MUSTOFA
JURNAL ILMU KEFARMASIAN INDONESIA Vol 10 No 1 (2012): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1918.329 KB)

Abstract

The root of pasak bumi (Eurycoma longifolia, Jack) is one of plant from Indonesia known as aphrodisiac. This study was conducted to evaluate the influence of pasak bumi standardized extract to testosterone level and histopathological changes of the testes in male Wistar rats. Sample were 50 adult male rats aged 3 4 months were divided into 5 groups. Group I (control) was given distilled water, group II (positive control), was given testosterone (Andriol ®), group III, IV, and V were each given a standardized extract of the roots of pasak bumi doses of 50, 100, and 200 mg/kg of body weight, respectively. The extract was given orally twice a day for six days and forty nine days and then testes was taken out on 7th and 50th day. Testosterone level was assayed on 7th and 50th day by the ELISA methods. The results were analyzed using one way ANOVA, and post hoc Dunnet (2-sided) (p < 0.05). The data of this study shows that treatment with water extract of pasak bumi root influence the number of Leydig cells that would also increase the concentration of testosterone in Wistar male rats positively at a dose of 100 mg/kg and 200 mg/kg body weight.
Efek Anti Diabetes Spirulina Platensis Terhadap Analisis Kadar, Gambaran Histopatologi, Ekspresi Insulin dan Glucose Transpoter 4 Pada Tikus Putih Wistar yang Diinduksi Streptozopin Kintoko Kintoko; Rifqi Ferry Balfas; Nura Ustrina; Sitarina Widyarini; Lintang Cahya Saputri; Anandita Nurwijayanti; Fajar Slamet Riana; Neni Tri Anggraini
JURNAL ILMU KEFARMASIAN INDONESIA Vol 16 No 2 (2018): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (331.578 KB) | DOI: 10.35814/jifi.v16i2.541

Abstract

Based on Basic Health Research, in Yogyakarta the incidence of diabetes mellitus based on doctor's diagnosis is 2.6% and symptoms will increase with age, but will decrease from age> 65 years. In the treatment of diabetes mellitus there are several treatments with synthetic drugs and natural ingredients for natural ingredients such as Spirulina platensis. Spirulina platensis is one of the microalgae that contains the natural spectrum of carotene and xantophyll pigment mixtures, and with fikocyanin has antioxidant activity, and Spirulina platensis can show a decrease in blood sugar. The type of research conducted is an experimental study. Spirulina platensis is made with several doses of 36 mg, 72 mg, and 144 mg. Rats were divided into 6 groups, 5 groups of streptozotosin induced at a dose of 45 mg / kgBW until the mice had DM were characterized by high KGD yield, then given spirulina suspension, measured in sugar levels on days 0-28. After that the mice in the blood and then analyzed the effect of Spirulina platensis on creatinine and urea in blood. The results were analyzed using SPSS ANOVA and Post Hoc Test methods with 95% confidence level. The results of the analysis of levels in various groups showed that in the induction of streptozotocin treated with spirulina plantesis various doses there was improvement in each analysis of the levels obtained, and also at the dose STZ+SP 36 in each analysis can improve the level of analysis. While on histopathology result and insulin expression in pancreas and histology and expression of glucose transporter 4 and histopathology on kidney and liver showed good change at various treatment dose group especially in STZ+SP 36 group. So at STZ+SP 36 dose showing the change both in this study.
Co-Authors . Harjadi . Muttaqien Abd. Rasyid Syamsuri Abdul Rohman Achmad Mursyidi Achmad Mursyidi Adrenalin, Sruti Listra Agnes Nora Iska Harnita Agnesia Endang Tri Hastuti Wahyuni Agung Endro Nugroho Agung Giri Samudra Agustina Dwi Wijayanti AGUSTINUS YUSWANTO Ahmad Mursyidi Ahmadi, Maulidina Alfarisa Nurrurozi Alfarisa Nururrozi Alfarisa Nururrozi Alfarisa Nururrozi Alfarisa Nururrozi Ana Sahara Anandita Nurwijayanti Andayana Puspitasari Gani Anna Ekawati Apriani, Lalily Arief Nurrochmad Asmarani Kusumawati Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu Claude Mona Airin Deasy Wulan Dwiratna Devi Usdiana Rosyidah Devita Anggraeni Dewajani Purnomosari Dewajani Purnomosari Dhasia Ramandani, Dhasia Dhirgo Adji Dimas Adhi Pradana Dwi Priyowidodo Edy Meiyanto Eka Kumalasari Endang Dwi Wulansari Erma Yunita Erwin . Erwin E Eryl Sri Rohayati Eti Nurwening Sholikhah Etriwati E Fajar Slamet Riana Fara Azzahra Fara Azzahra FARIDA HAYATI Fauziah, Ima Frans J.M. Harren Galih Dwi Mulyati Gede Bayu Suparta Gemini Alam Geovani Meryza Oka Putra Caesar Gharsina Ghaisani Yumni Hady Anshory Hardian, Andreas Bandang Harjadi Harjadi Hary Purnamaningsih Hary Purnamaningsih Hastari Wuryastuty Hayati, Farida Helmina Wati Hizriah Alief Jainudin Ign Edi Santosa Ika Tidariani Imanjati, Lynda Nugrahaning Indwiani Astuti Indwiani Astuti Irianiwati Irianiwati Irianiwati Widodo Iwan Sahrial Hamid Joko Prastowo Jumina Jumina Kintoko, Kintoko Kony Putriani Kurniasih . Kurniasih Kurniasih Kurniasih Kurniasih Laela Hayu Nurani Laela Hayu Nurani Laela Hayu Nurani Laela Hayu Nurani Laksono Trisnantoro Lalily Apriani Laras Widawaty Putri Lintang Cahya Saputri Lukman Hakim Lukman Hakim Marchaban Marchaban Mega Cahya Nalasukma Michael Haryadi Wibowo Mustofa Mustofa Mustofa Mustofa Nanang Fakhrudin, Nanang Nemay Anggadewi Ndaong Neni Tri Anggraini Ngatidjan Ngatidjan Ni'ma, Neli Syahida Nina Salamah Noor Ardhi Pratomo Novrita Padauleng Novrita Padauleng Nugrahani, Warih Pulung Nura Ustrina Nurman Haribowo Pramono, Suwidjiyo Puspa Wikan Sari R Wasito R Wasito Rahmawati, Irhamna Putri Ramadhani, Mungky Ema Ranita Tri Budi M Rifqi Ferry Balfas Rini Widayanti Risfah Yulianty Risfah Yulianty Riska Nufika Riska Nufika Ruslin Hadanu Rusmihayati Rusmihayati Santika, Luh Putu Nadya Santoso, Ferdinand Prayogo Cahyo Sardjiman S Siti Salma Yusuf Slamet Raharjo Slamet Raharjo Soedarmanto Indarjulianto Soedarmanto Indarjulianto Soedarmanto Indarjulianto Sri Herwiyanti Sri Herwiyanti, Sri Subagus Wahyuono Subagus Wahyuono Sudarsono Sudarsono Sudibyo Martono Sugiyanto - Sugiyanto . Sugiyanto Sugiyanto Sugiyanto Sugiyanto Sugiyanto, Sugiyanto Sugiyono Sugiyono Sugiyono Sugiyono Sugiyono Sugiyono Sugiyono Sultan Suhrah Febrina Karim Suwidjiyo Pramono Suwidjiyo Pramono Suwidjiyo Pramono Suwidjiyo Pramono Tiaravista, Amanda Gita Tri Untari Tri Untari Tri Untari Tri Wahyu Pangestiningsih Tria Zakinah Vembriarto Jati Pramono Vinsa Cantya Prakasita Wahyu Widiyaningsih Wahyu Widyaningsih Wahyu Widyaningsih Widhihastuti, Endah Winarsih, Sugi Yanuartono . Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono yanuartono, yanuartono - Yeremia Yobelino Sitompul Yuli Purwandari Kristiangingrum Yuli Purwandari Kristianingrum Yuli Purwandari Kristianingrum, Yuli Purwandari Yunitasari, Maria Yustina Sri Hartini Yusuf, Siti Salma