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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Buletin Hama dan Penyakit Tumbuhan Agrikultura Jurnal Natur Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Hortikultura Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat AGRIVITA, Journal of Agricultural Science Journal of ICT Research and Applications Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Agrotek Tropika JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Entomologi Indonesia Agro Bali: Agricultural Journal Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Proceedings Series on Physical & Formal Sciences

SRI HENDRASTUTI HIDAYAT

Departemen Proteksi Tanaman, Fakultas Pertanian, IPB Jl. Kamper Kampus IPB Dramaga, Bogor 16680

Author-ID : 385601

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Veterinary

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Dual Infection of Pepper Yellow Leaf Curl Virus and Chilli Veinal Mottle Virus in Causing the Yellow leaf Curl Disease on Chili Trisno, Jumsu; Jamsari, Jamsari; Hidayat, Sri Hendrastuti
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 5 No. 2 (2021): December 2021
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.5.2.77-88.2021

Yellow leaf curl disease is the primary disease in chili plants with up to 100% losses. Detection from the field showed a mixed infection between Geminivirus or Pepper Yellow Leaf Curl Virus (PYLCV) and several viruses, including Chilli Veinal Mottle Virus (ChiVMV) and Cucumber mosaic Virus (CMV). Therefore, this study aimed to determine the role of PYLCV and ChiVMV dual infection in influencing the development of chili yellow leaf curl disease. This study used a completely randomized design (CRD) with six treatments and ten replications. The treatments used were multiple Geminiviruses and ChiVMV infections simultaneously, before ChiVMV co-infection, after Geminivirus infection, single infection and whithout infection viruses. The results showed that the presence of ChiVMV infection before, after, and concurrently with Geminiviruses infection could increase the yellow leaf curl disease intensity and reduce plant height and wet weight growth. The mean scale value of ChiVMV infection before, after, and concurrently with Geminiviruses infection were 3.38, 3.90 and 3.58 compared to single Geminiviruses infection (scale of 3.20) and single ChiVMV (scale of 1.33). The interaction analysis of ChiVMV and geminiviruses infections based on the percentage reduction in plant growth height and wet weight showed additive interaction. ChiVMV co-infection further exacerbated the incidence of yellow leaf curl diseases in chilies.

Ketahanan 22 Genotipe Cabai (Capsicum spp.) terhadap Phytophthora capsici Leonian dan Keragaman Genetiknya Yunianti, Rahmi; Sastrosumarjo, Sarsidi; Sujiprihati, Sriani; Surahman, Memen; Hidayat, Sri Hendrastuti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 2 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Laboratory and field experiments were carried out to analyze genetic diversity of 22 pepper genotypes (Capsicum spp.) and their resistance to Phytophthora capsici Leonian. Resistance screening was performed in plastic flats 72 cells. Inoculation was done on 28-day old pepper plant soon after watering by pipeting 5 ml of inoculum (105 zoospore/ml) at the base of each plant. P. capsici isolate used in this experiment was TG01, identified as race 3 based on AVRDC differential pepper lines (PI 188478, PBC 602, PBC 137 and Early Calwonder). The evaluation of pepper genotypes characteristic was conducted in the field. Principle Component Analysis, Clustering Analysis and Biplot Analysis were used to analyze genetic diversity based on 37 characters. Result of resistance evaluation showed that two genotypes (C4 and C13) were identified as resistant, 7 genotypes (C2, C3, C5, C8, C10, C15, and C20) as slightly resistant, 6 genotypes (C7, C9, C17, C19, C21 and C27) as slightly susceptible, and 7 genotypes (C1, C11, C18, C28, C48, C64, and C65) as susceptible. Based on genetic diversity analyzed, all genotypes could be divided into 4 clusters. Cluster I consisted of 18 genotypes i.e. C1, C2, C3, C4, C5, C7, C8, C9, C10, C13, C14, C15, C17, C18, C19, C28, C64, and C65. Cluster II consisted of only 1 genotype i.e. C48. Cluster III consisted of 2 genotypes (C20 and C21) which were characterized by the colour of corolla, corolla spot, and filament. Cluster IV consisted of 1 genotype (C27) which was characterized by fruit cross-sectional corrugation. Key words : Capsicum, resistance, Phytophthora capsici, genetic diversity, clustering.

Daya Gabung dan Heterosis Ketahanan Pepaya (Carica papaya L) terhadap Penyakit Antraknosa*) Hafsah, Siti; astrosumarjo, Sarsidi S; Sujiprihati, Sriani; Sobir, ,; Hidayat, Sri Hendrastuti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 3 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

This study was conducted to determine the relative importance of general (GCA) and specific (SCA) combining ability and heterosis effects on resistance to C. gloeosporioides in selected papaya genotypes. A half diallel mating scheme of five genotypes of papaya were evaluated in the field. The combining ability analysis revealed that both the additive and nonadditive gene effects were present. Crosses between the resistance and susceptible genotypes showed intermediate disease reaction to papaya anthracnose disease (PAD) suggesting a polygenic system of resistance to the disease. Highly resistance heterosis of 51.51% at Tajur and 48.71% at Gunung Geulis were expressed in crosses between IPB5 x PB000174. Key words: Papaya, anthracnose, GCA, SCA, heterosis

Kriteria Seleksi untuk Perakitan Varietas Cabai Tahan Phytophthora capsici Leonian Yunianti, Rahmi; Sastrosumarjo, Sarsidi; Sujiprihati, Sriani; Surahman, Memen; Hidayat, Sri Hendrastuti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Selection criteria for developing Phytophthora blight-resistant varieties of pepper had been done in both laboratory and ﬁ eld conditions. Resistance screening were conducted on 28-days-old pepper plants grown in 72-cell ﬂ ats by inoculating 5 mL of inoculum (contain 105 zoospore mL-1) to the base of each plant. Phytophthora capsici isolate used in this experiment was TG01, which then identiﬁ ed as race-3. The evaluation of pepper genotypes characteristics were conducted in ﬁ eld. Eight characters demonstrated a wide genetic variability; those characters were resistance to Phytophthora blight, infection period, dicotomous height, canopy width, fruit weight, fruit length, fruit diameter and production. These characters along with fruit wall thickness, chlorophyll-a, chlorophyll-b, total chlorophyll had high heritability values. Using path analysis infection period, dicotomous height and fruit diameter are recommended to be simultaneously used as selection criteria for developing Phytophthora blight resistant pepper varieties. Keywords : capsicum, Phytophthora capsici, selection criteria, path analysis

Pengaruh Perlakuan Ethyl Methane Sulfonate pada Tanaman Cabai (Capsicum annuum L.) dan Ketahanannya terhadap Chilli Veinal Mottle Virus (ChiVMV) Manzila, Ifa; Hidayat, Sri Hendrastuti; Mariska, Ika; Sujiprihati, Sriani
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 3 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Ethyl Methane Sulfonate (EMS) may induce mutation leading to somaclonal variation if it is used at the appropriate combination of EMS concentration and exposure time. Variation in somaclonal might be valuable as a source of resistance to plant pathogens including plant viruses. This study was aimed 1) to determine the optimum EMS concentration and incubation time that may induce somaclonal variation in chilli pepper; and 2) to evaluate the resistance of the somaclone to ChiVMV infection. Shoot-tip explants of fi ve chilli pepper genotypes (Jatilaba, ICPN 12#4, PBC495, Helem, and Gelora) were treated with EMS at combination of different concentrations (0.25%, 0.5% 1.0% and control), and incubation time (15, 30, 60 min). Subsequently, each explant was grown in multiplication media (MS media + 5 mg L -1 BAP + 0.5 mg L-1 TDZ), rooting media (MS media + 1 mg L-1 NAA), and acclimatization media (mixture of soil : sand : compost 2:1:1 w/w). Our results showed that the higher EMS concentration and the longer incubation period the smaller the number of survive explants. The highest survival rate 20.4 % was achieved with 0.5% EMS in combination with 60 min incubation period. This treatment combination also showed induction of phenotypic variation. Two somaclonal plants derived from Gelora genotype, designated as somaclones K1 and K2, survived until fruit development and maturation. A total of 245 progenies of K1 and 243 progenies of K2, respectively were evaluated for their resistance to ChiVMV infection through mechanical inoculation using ChiVMV-Cikabayan isolate. Following the detection of ChiVMV using DAS-ELISA, it was confi rmed that 4.09% of the somaclonal progenies were resistance to ChiVMV. Keywords: Capsicum annuum L., ChiVMV, ethyl methane sulfonate, induce mutation, resistance

Molecular Characterization of Begomovirus Infecting Yard Long Bean (Vigna unguiculata subsp. sesquipedalis L.) in Java, Indonesia Nurulita, Sari; Hidayat, Sri Hendrastuti; Mutaqin, Kikin Hamzah; Thomas, John
BIOTROPIA Vol. 22 No. 1 (2015): BIOTROPIA Vol. 22 No. 1 June 2015
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2015.22.1.401

Begomovirus has been identified as one of the causal agents associated with yellow mosaic disease in yardlong bean (Vigna unguiculata subsp. sesquipedalis (L.)) in Java. Previous studies reported that Begomovirus has infected several Leguminosae species in South Asia, and several Begomoviruses have also been reported to infect important crops in Indonesia based on nucleotide sequence characterization. This study was conducted to identify and characterize Begomovirus isolated from yardlong bean samples in Java based on specific genomic features of the Common Region. Samples were collected from yardlong bean fields in Central Java (Tegal, Magelang, and Klaten), Yogyakarta (Sleman), and West Java (Bogor and Subang); viral detection was performed using I-ELISA, PCR, and sequencing; and molecular characterization was conducted using BioEdit v.7.0.5 and MEGA 6.06 software. Yellow mosaic disease was observed in nearly all surveyed fields, and infection of Potyvirus and Begomovirus was detected either as single or mixed infection. PCR results confirmed Begomovirus infection in samples from Tegal, Klaten, Magelang, Subang, and Bogor. Sequence analysis revealed that the virus infecting yardlong bean was Mungbean Yellow Mosaic India Virus (MYMIV) and shared close genetic similarity with isolates from Bangladesh, India, Pakistan, and Nepal. Further analysis revealed conserved regions surrounding the Common Region, including the “TATA box” sequence, hairpin-loop structure, repetitive sequences, and the conserved nonanucleotide TAATATTAC. This study provides the first report of MYMIV infection in Indonesia.

Co-Authors . SUDARSONO Abdul Muin Adnan ABDUL MUNIF ALI NURMANSYAH Amelia Feryna Bulan Dini Ana Septiana Saputri Anas Dinurrohman Susila Aqlima , Aqlima, nFN Arifin Tasrif Asmar Hasan Asniwita Asniwita Astri Windia Wulandari Wulandari ATI SRI DURIAT Awang Maharijaya Ayu Kartini Parawansa Bambang S. Purwoko Bambang Sapta Purwoko Bonny Poernomo Wahyu Soekarno Bonny Poernomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Budi Tjahjono Darni Rambu D. Siala Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Diny Dinarti Dono Wahyuno Dono Wahyuno Dwi Astuti Dwi Subekti DWI SUBEKTI Dwi Wiyati Nurul Septariani Dwiwiyati Nurul Septariani Efendi, Darda Efi Toding Tondok Eliza Suryati Rusli Endang Nurhayati Endang Nurhayati Evan P. Ramdan Farida, Naimatul Fitrianingrum Kurniawati, Fitrianingrum Gede Suastika Gede Suastika GEDE SUASTIKA GEDE SUASTIKA Gede Suastika Gede Suastika Giyanto Giyanto Giyanto Hamdayanty Hamdayanty Hanif, Andini Hari Priwiratama Harwan Susetio Heriyanto Syafutra I Wayan Winasa Ifa Manzila Ifa Manzila Ika Mariska Ika Mariska Irsan Nuhantoro Ishak Manti ISHAK MANTI Isti Wulandari Jamsari Jamsari Jati Adiputra John Thomas, John Joni Hidayat, Joni JUMANTO HARJOSUDARMO Jumanto Harjosudarmo Jumanto Harjosudarmo Jumsu Trisno Kadwati Kadwati Kikin H Mutaqin KIKIN HAMZAH MUTAQIN Ladja, Fausiah T. Laksono Trisnantoro Listihani, Listihani Mawarni, Sofi Meity S Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Melinda . Meliyana Memen Surahman Miftakhurohmah Miftakhurohmah Miftakhurohmah Mimi Sutrawati Muh. Taufik Muhamad Syukur Muhammad Herman Muhammad Taufik Muhammad Taufik MUHAMMAD TAUFIK Nada, Azmi Khoirin Neni Gunaeni Niken Nur Kasim, Niken Nur Nissa Fawwaz Adilah NOOR AIDAWATI ORAWAN CHATCHAWANKAN PANICH Prabawati Hyunita Putri Puji Lestari PURNAMA HIDAYAT Purwoko, Bambang Sapto Purwono Purwono Rahayuwati, Sat RAHMI YUNIANTI Ramdan, Evan Purnama RAUF, AUNU Redy Gaswanto Redy Gaswanto, Redy Refa Yulianingsih Reymas M.R. Ruimassa Rina Rachmawati Rita Noveriza Rita Noveriza RITA NOVERIZA Rita Noveriza Rizqiyah, Sakinah Inayatur Rokhana Faizah Roy Ibrahim RR. Ella Evrita Hestiandari Rusmilah Suseno RUSMILAH SUSENO Rustiani, Ummu S. Saiful Akhyar Lubis Sari Nurulita Sari Nurulita Sari, Rahmah Dian Sarsidi S astrosumarjo Sarsidi Sastrosumarjo Satya Nugroho Sayekti, Tri Wahono Dyah Ayu Sherli Anggraini Sientje Mandang Sumaraw Siregar, Ivan Arif Rachman Martua Siti Hafsah Siti Shofiya Nasution Slamet Susanto Sobir Sobir Soemartono Sosromarsono Soemartono Sosromarsono Sri Hartati Sri Hartati Sri Hartati Sri Sulandari Sri Sulandari Sri Sulandari Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati SRIANI SUJIPRIHATI Sugeng Santoso Supramana Suryo Wiyono Susanti Mugi Lestari Tega Kintasari Titiek YULIANTI TRI ASMIRA DAMAYANTI TRI JOKO SANTOSO Trikoesoemaningtyas Trimuri Habazar TRIMURTI HABAZAR Triyani Dumaria Tutik Harmiyati, Tutik Ummu S. Rustiani Ummu Salamah Rustiani Utomo Kartosuwondo UTOMO KARTOSUWONDO Utomo Kartosuwondo UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO Vinsen Willi Wardhana Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Willing Bagariang Zahratul Millah

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