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Departemen Proteksi Tanaman, Fakultas Pertanian, IPB Jl. Kamper Kampus IPB Dramaga, Bogor 16680
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Efektivitas Gelombang Ultrasonik dalam Eliminasi Nematoda Daun Aphelenchoides pada Umbi Bawang Merah Kurniawati, Fitrianingrum; Supramana, Supramana; Hidayat, Sri Hendrastuti; Tondok, Efi Toding; Syafutra, Heriyanto; Damayanti, Tri Asmira; Mubin, Nadzirum
Jurnal Agrikultura Vol 37, No 1 (2026): April, 2026
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v37i1.68192

Abstract

Nematoda daun Aphelenchoides merupakan salah satu patogen tanaman yang memiliki kisaran inang yang luas dan menyebabkan kehilangan hasil panen. Metode konvensional telah sering digunakan dalam pengendalian nematoda termasuk nematisida kimia, perawatan air panas, dan nematisida hayati tetapi memiliki keterbatasan. Hal ini memerlukan eksplorasi metode alternatif yang ramah lingkungan dan efektif, seperti teknologi gelombang ultrasonik. Penelitian bertujuan mengetahui efektivitas gelombang ultrasonik dalam eliminasi nematoda daun Aphelenchoides pada umbi bawang serta pengaruh pada pertumbuhan umbi bawang. Ekstraksi nematoda menggunakan metode pengabutan (mist chamber) dan perendaman air dingin. Nematoda diidentifikasi berdasarkan karakter morfologi. Umbi bawang merah yang telah diinfestasikan Aphelenchoides diberi perlakuan ultrasonik dengan lama perlakuan selama 26, 30, 37, 44, 60 menit dan kontrol. Umbi bawang yang telah diberi perlakuan ultrasonik ditanam di pot dan diamati parameter pertumbuhannya sampai panen. Data ditabulasikan di Microsoft Excel dan diolah menggunakan SAS JMP. Perlakuan ultrasonik dengan durasi yang lebih dari 37 menit menghasilkan tingkat mortalitas yang tinggi (≥84,00%).  Waktu papar yang berbeda menunjukkan pengaruh yang tidak berbeda nyata dengan kontrol pada tinggi tanaman dan panjang akar. Lebih lanjut, perlakuan ultrasonik dengan durasi yang lebih lama (≥ 37 menit) sangat efektif dalam menekan populasi nematoda Aphelenchoides tanpa memengaruhi pertumbuhan tanaman bawang merah.
Ketahanan 22 Genotipe Cabai (Capsicum spp.) terhadap Phytophthora capsici Leonian dan Keragaman Genetiknya Rahmi Yunianti; Sarsidi Sastrosumarjo; Sriani Sujiprihati; Memen Surahman; Sri Hendrastuti Hidayat
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 2 (2007): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.865 KB) | DOI: 10.24831/jai.v35i2.1318

Abstract

Laboratory and field experiments were carried out to analyze genetic diversity of 22 pepper genotypes (Capsicum spp.) and their resistance to Phytophthora capsici Leonian. Resistance screening was performed in plastic flats 72 cells. Inoculation was done on 28-day old pepper plant soon after watering by pipeting 5 ml of inoculum (105 zoospore/ml) at the base of each plant. P. capsici  isolate used in this experiment was TG01, identified as race 3 based on AVRDC differential pepper lines (PI 188478, PBC 602, PBC 137 and Early Calwonder).  The evaluation of pepper genotypes characteristic was conducted in the field.  Principle Component Analysis, Clustering Analysis and Biplot Analysis were used to analyze genetic diversity based on 37 characters. Result of resistance evaluation showed that two genotypes (C4 and C13) were identified as resistant, 7 genotypes (C2, C3, C5, C8, C10, C15, and C20) as slightly resistant, 6 genotypes (C7, C9, C17, C19, C21 and C27) as slightly susceptible, and 7 genotypes (C1, C11, C18, C28, C48, C64, and C65) as susceptible.  Based on genetic diversity analyzed, all genotypes could be divided into 4 clusters.  Cluster I consisted of 18 genotypes i.e. C1, C2, C3, C4, C5, C7, C8, C9, C10, C13, C14, C15, C17, C18, C19, C28, C64, and C65. Cluster II consisted of only 1 genotype i.e. C48.  Cluster III consisted of 2 genotypes (C20 and C21) which were characterized by the colour of corolla, corolla spot, and filament. Cluster IV consisted of 1 genotype (C27) which was characterized by fruit cross-sectional corrugation.   Key words :  Capsicum, resistance, Phytophthora capsici, genetic diversity, clustering.
Daya Gabung dan Heterosis Ketahanan Pepaya (Carica papaya L) terhadap Penyakit Antraknosa*) Siti Hafsah; Sarsidi S astrosumarjo; Sriani Sujiprihati; , Sobir; Sri Hendrastuti Hidayat
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 3 (2007): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.372 KB) | DOI: 10.24831/jai.v35i3.1331

Abstract

This study was conducted to determine the relative importance of general (GCA) and specific (SCA) combining ability and heterosis effects on resistance to C. gloeosporioides in selected papaya genotypes. A half diallel mating scheme of five genotypes of papaya were evaluated in the field. The combining ability analysis revealed that both the additive and nonadditive gene effects were present.  Crosses between the resistance and susceptible genotypes showed intermediate disease reaction to papaya anthracnose disease (PAD) suggesting a polygenic system of resistance to the disease. Highly resistance heterosis of 51.51% at Tajur and 48.71% at Gunung Geulis were expressed in crosses between IPB5 x PB000174.   Key words: Papaya, anthracnose, GCA, SCA, heterosis
Kriteria Seleksi untuk Perakitan Varietas Cabai Tahan Phytophthora capsici Leonian Rahmi Yunianti; Sarsidi Sastrosumarjo; Sriani Sujiprihati; Memen Surahman; Sri Hendrastuti Hidayat
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.721 KB) | DOI: 10.24831/jai.v38i2.1796

Abstract

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Pengaruh Perlakuan Ethyl Methane Sulfonate pada Tanaman Cabai (Capsicum annuum L.) dan Ketahanannya terhadap Chilli Veinal Mottle Virus (ChiVMV) Ifa Manzila; Sri Hendrastuti Hidayat; Ika Mariska; Sriani Sujiprihati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 3 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.474 KB) | DOI: 10.24831/jai.v38i3.14250

Abstract

Ethyl Methane Sulfonate (EMS) may induce mutation leading to somaclonal variation if it is used at the appropriate combination of EMS concentration and exposure time. Variation in somaclonal might be valuable as a source of resistance to plant pathogens including plant viruses. This study was aimed 1) to determine the optimum EMS concentration and incubation time that may induce somaclonal variation in chilli pepper; and 2) to evaluate the resistance of the somaclone to ChiVMV infection. Shoot-tip explants of fi ve chilli pepper genotypes (Jatilaba, ICPN 12#4, PBC495, Helem, and Gelora) were treated with EMS at combination of different concentrations (0.25%, 0.5% 1.0% and control), and incubation time (15, 30, 60 min). Subsequently, each explant was grown in multiplication media (MS media + 5 mg L -1 BAP + 0.5 mg L-1 TDZ), rooting media (MS media + 1 mg L-1 NAA), and acclimatization media (mixture of soil : sand : compost 2:1:1 w/w). Our results showed that the higher EMS concentration and the longer incubation period the smaller the number of survive explants. The highest survival rate 20.4 % was achieved with 0.5% EMS in combination with 60 min incubation period. This treatment combination also showed induction of phenotypic variation. Two somaclonal plants derived from Gelora genotype, designated as somaclones K1 and K2, survived until fruit development and maturation. A total of 245 progenies of K1 and 243 progenies of K2, respectively were evaluated for their resistance to ChiVMV infection through mechanical inoculation using ChiVMV-Cikabayan isolate. Following the detection of ChiVMV using DAS-ELISA, it was confi rmed that 4.09% of the somaclonal progenies were resistance to ChiVMV. Keywords: Capsicum annuum L., ChiVMV, ethyl methane sulfonate, induce mutation, resistance
Co-Authors . SUDARSONO Abdul Muin Adnan ABDUL MUNIF ALI NURMANSYAH Amelia Feryna Bulan Dini Ana Septiana Saputri Anas Dinurrohman Susila Aqlima , Aqlima, nFN Arifin Tasrif Asmar Hasan Asniwita Asniwita Astri Windia Wulandari Wulandari ATI SRI DURIAT Ayu Kartini Parawansa Bambang S. Purwoko Bambang Sapta Purwoko Bonny Poernomo Wahyu Soekarno Bonny Poernomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Budi Tjahjono Darni Rambu D. Siala Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Diny Dinarti Dono Wahyuno Dono Wahyuno Dwi Astuti DWI SUBEKTI Dwi Subekti Dwi Wiyati Nurul Septariani Dwiwiyati Nurul Septariani Efendi, Darda Efi Toding Tondok Eliza Suryati Rusli Endang Nurhayati Endang Nurhayati Evan P. Ramdan Farida, Naimatul Fitrianingrum Kurniawati, Fitrianingrum Gede Suastika GEDE SUASTIKA Gede Suastika Gede Suastika GEDE SUASTIKA Gede Suastika Giyanto Giyanto Giyanto Hamdayanty Hamdayanty Hanif, Andini Hari Priwiratama Harwan Susetio Heriyanto Syafutra I Wayan Winasa Ifa Manzila Ifa Manzila Ika Mariska Ika Mariska Irsan Nuhantoro Ishak Manti ISHAK MANTI Isti Wulandari Jamsari Jamsari Jati Adiputra Joni Hidayat, Joni Jumanto Harjosudarmo Jumanto Harjosudarmo JUMANTO HARJOSUDARMO Jumsu Trisno Kadwati Kadwati Kikin H Mutaqin KIKIN HAMZAH MUTAQIN Ladja, Fausiah T. Laksono Trisnantoro Listihani, Listihani Mawarni, Sofi Meity S Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Melinda . Meliyana Memen Surahman Miftakhurohmah Miftakhurohmah Miftakhurohmah Mimi Sutrawati Mubin, Nadzirum Muh. Taufik Muhamad Syukur Muhammad Herman MUHAMMAD TAUFIK Muhammad Taufik Muhammad Taufik Nada, Azmi Khoirin Neni Gunaeni Niken Nur Kasim, Niken Nur Nissa Fawwaz Adilah NOOR AIDAWATI ORAWAN CHATCHAWANKAN PANICH Prabawati Hyunita Putri Puji Lestari PURNAMA HIDAYAT Purwoko, Bambang Sapto Purwono Purwono Rahayuwati, Sat Rahmi Yunianti Rahmi Yunianti Ramdan, Evan Purnama RAUF, AUNU Redy Gaswanto Redy Gaswanto, Redy Refa Yulianingsih Reymas M.R. Ruimassa Rina Rachmawati Rita Noveriza Rita Noveriza RITA NOVERIZA Rita Noveriza Rizqiyah, Sakinah Inayatur Rokhana Faizah Roy Ibrahim RR. Ella Evrita Hestiandari Rusmilah Suseno RUSMILAH SUSENO Rustiani, Ummu S. Saiful Akhyar Lubis Sari Nurulita Sari Nurulita Sari, Rahmah Dian Sarsidi S astrosumarjo Sarsidi Sastrosumarjo Sarsidi Sastrosumarjo Satya Nugroho Sherli Anggraini Sientje Mandang Sumaraw Siregar, Ivan Arif Rachman Martua Siti Hafsah Siti Shofiya Nasution Slamet Susanto Sobir Sobir Soemartono Sosromarsono Soemartono Sosromarsono Sri Hartati Sri Hartati Sri Hartati Sri Sulandari Sri Sulandari Sri Sulandari Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Sugeng Santoso Supramana Suryo Wiyono Susanti Mugi Lestari Tega Kintasari Titiek YULIANTI TRI ASMIRA DAMAYANTI TRI JOKO SANTOSO Trikoesoemaningtyas Trimuri Habazar TRIMURTI HABAZAR Triyani Dumaria Tutik Harmiyati, Tutik Ummu S. Rustiani Ummu Salamah Rustiani UTOMO KARTOSUWONDO Utomo Kartosuwondo Utomo Kartosuwondo UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO Vinsen Willi Wardhana Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Willing Bagariang Zahratul Millah