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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Buletin Hama dan Penyakit Tumbuhan Agrikultura Jurnal Natur Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Hortikultura Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat AGRIVITA, Journal of Agricultural Science Journal of ICT Research and Applications Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Agrotek Tropika JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Entomologi Indonesia Agro Bali: Agricultural Journal Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Proceedings Series on Physical & Formal Sciences
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Departemen Proteksi Tanaman, Fakultas Pertanian, IPB Jl. Kamper Kampus IPB Dramaga, Bogor 16680
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Infeksi Ganda Pepper Yellow Leaf Curl Virus dan Chilli Veinal Mottle Virus dalam Menimbulkan Penyakit Daun Kuning Keriting Cabai Jumsu Trisno; Jamsari Jamsari; Sri Hendrastuti Hidayat
Jurnal Proteksi Tanaman Vol 5 No 2 (2021): December 2021
Publisher : Plant Protection Departement, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.5.2.77-88.2021

Abstract

Yellow leaf curl disease is the primary disease in chili plants with up to 100% losses. Detection from the field showed a mixed infection between Geminivirus or Pepper Yellow Leaf Curl Virus (PYLCV) and several viruses, including Chilli Veinal Mottle Virus (ChiVMV) and Cucumber mosaic Virus (CMV). Therefore, this study aimed to determine the role of PYLCV and ChiVMV dual infection in influencing the development of chili yellow leaf curl disease. This study used a completely randomized design (CRD) with six treatments and ten replications. The treatments used were multiple Geminiviruses and ChiVMV infections simultaneously, before ChiVMV co-infection, after Geminivirus infection, single infection and whithout infection viruses. The results showed that the presence of ChiVMV infection before, after, and concurrently with Geminiviruses infection could increase the yellow leaf curl disease intensity and reduce plant height and wet weight growth. The mean scale value of ChiVMV infection before, after, and concurrently with Geminiviruses infection were 3.38, 3.90 and 3.58 compared to single Geminiviruses infection (scale of 3.20) and single ChiVMV (scale of 1.33). The interaction analysis of ChiVMV and geminiviruses infections based on the percentage reduction in plant growth height and wet weight showed additive interaction. ChiVMV co-infection further exacerbated the incidence of yellow leaf curl diseases in chilies.
Kemampuan Kolonisasi Cendawan Endofit dan Peningkatan Pertumbuhan Bibit Cabai Evan Purnama Ramdan; Efi Toding Tondok; Suryo Wiyono; Sri Hendrastuti Hidayat; Widodo Widodo
Proceedings Series on Physical & Formal Sciences Vol. 2 (2021): Prosiding Seminar Nasional Fakultas Pertanian dan Perikanan
Publisher : UM Purwokerto Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (395.798 KB) | DOI: 10.30595/pspfs.v2i.175

Abstract

Endophytic fungi are fungi that live in healthy plant tissues without causing disease symptoms. Endophytic fungi in chili have been tested as both biocontrol agents and growth promoters, but their colonization has not been reported. Therefore, this study aimed to determine the level of colonization of endophytic fungi and its effect on the growth of chili seedlings. A total of 8 endophytic fungi were prepared at a density of 2.8 × 106 CFU mL-1. Then the endophytic fungus was inoculated 2 times, first by soaking the seeds, and secondly by watering the endophytic fungus suspension on chili seedlings aged 3 weeks after sowing. Endophytic fungi were re-isolated on chili seedlings that were 4 weeks old after sowing on the roots and stems to determine their colonization ability. Chili seeds were then maintained for up to 4 weeks after transplanting to observe their growth. The results showed that the endophytic fungal colonization ranged from 26-60% on the chili root, while at the base of the stem it was 20-40% with a different pattern of colonization distribution. In addition, endophytic fungus colonization was also able to increase the shoot height and root length of chili seedlings.
Identification of mealybugs on Piper nigrum as vector of Piper yellow mottle virus (Badnavirus: Caulimoviridae) Miftakhurohmah Miftakhurohmah; Sri Hendrastuti Hidayat; Kikin Hamzah Mutaqin; Bonny Poernomo Wahyu Soekarno; Dono Wahyuno
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 22 No. 2 (2022): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.222144-153

Abstract

Piper yellow mottle virus (PYMoV) is the dominant virus that causes mottle disease in black pepper (Piper nigrum). Two species of mealybugs, Ferrisia virgata and Planococcus minor have been reported as vectors of PYMoV. A different species of mealybug that has never been reported before was found in black pepper. Molecular approaches including total DNA isolation of mealybug collected from the field, mealybugs identification by DNA barcode, detection of PYMoV in single mealybugs, were conducted as an approach to identify the potential of mealybugs as PYMoV vector in the field. Mealybugs were collected from black pepper plants in Cimanggu (Bogor, West Java) and Sukamulya (Sukabumi, West Java). Characters of adult females were observed for morphological identification. Molecular-based identification of the mealybugs and PYMoV involved the following procedures: total DNA isolation, DNA amplification, nucleotide sequencing and sequence analysis. Three species of mealybugs, P. minor, F. virgata and Paracoccus marginatus were confirmed by morphological and molecular identification. This is the first report for the occurrence of P. marginatus in black pepper plants. PYMoV was successfully detected from field samples of F. virgata, P. minor and P. marginatus. This finding indicates the potential of insect vectors for disease spread and distribution.
Characteristics of Virus Symptoms in Chili Plants (Capsicum frutescens) Based on RGB Image Analysis Asmar Hasan; Widodo Widodo; Kikin Hamzah Mutaqin; Muhammad Taufik; Sri Hendrastuti Hidayat
AGRIVITA, Journal of Agricultural Science Vol 44, No 3 (2022)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i0.3731

Abstract

Virus infection in chili plants may cause various symptoms. The complexity of the symptoms and human vision ability often become limiting factors during disease investigations. Digital image analysis is expected to become a method to assist in comprehensively describing the symptoms of plant viruses. A disease survey was conducted on cayenne pepper fields in Southeast Sulawesi Province to observe symptoms of virus infection virtually and to record the symptomatic plant using an RGB camera. The split-channel method is used to process images, followed by multidimensional scaling statistical analysis. Later on, viruses associated with plants were detected serologically. Single or mixed infection of Tobacco mosaic virus, Cucumber mosaic virus, Chili veinal mottle virus, and Pepper mottle virus was confirmed by plant leaves showing yellow-mosaic and mottle symptoms. The digital image analysis method could show variations in the characteristics of symptoms based on digital numbers in that cannot be recognized based on the observation of visual symptoms. A new approach to study the interactions between plant infecting viruses and their effects based on image analysis has also been developed during this research. This method needs to be further validated through testing under controlled conditions, such as inoculating plants with a predetermined type of virus.
Meristem Culture Method for Virus Elimination from Shallot Bulb Prabawati Hyunita Putri; Diny Dinarti; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 16 No 4 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.4.185-189

Abstract

Meristem Culture Method for Virus Elimination from Shallot Bulb Shallot farmers in Indonesia have commonly grown bulbs from the previous planting season as seed sources for the next season. This may cause the accumulation of viruses in bulbs which in turn lowering plant productivity. A research was conducted to optimize the meristem culture method combined with thermotherapy (30 ºC) to produce virus-free shallot bulbs. Shallot bulbs of cv. Bima Curut that had been confirmed infected by Potyvirus and Carlavirus were used as explant sources. Bulb sterilization was carried out using bactericides, fungicides, and sodium hypochlorite. Meristem shoots of 0.6 to 0.7 mm in size were isolated from bulbs and plant successively in the shoot, root, and bulb induction medium to form mini bulbs. The mini bulbs were then planted in the screen house for 2 to 2.5 months until micro bulbs were formed. Virus detection by reverse transcription-polymerase chain reaction method confirmed that plantlets at the multiplication stage and plants in the screen house were free of Potyvirus and Carlavirus infestations. This study showed that the meristem culture method combined with thermotherapy is a potential approach for producing virus-free shallot bulbs.
Identification of Phytoplasmas on Carrot (Daucus carota L.) and Leafhopper Associated with Yellow Disease in Bogor and Bandung, West Java Isti Wulandari; Kikin Hamzah Mutaqin; Giyanto Giyanto; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 16 No 4 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.4.157-165

Abstract

Gejala kuning mirip seperti infeksi fitoplasma ditemukan pada pertanaman wortel di Jawa Barat. Penelitian bertujuan mengidentifikasi fitoplasma pada tanaman wortel dan serangga wereng yang berasosiasi dengan gejala kuning di Bogor dan Bandung. Wereng yang ditemukan pada pertanaman wortel di Bogor terdiri atas 5 spesies wereng daun (Hemiptera: Cicadellidae), yaitu Balclutha incisa (Matsumura), Orosius argentatus (Evans), Cicadulina bipunctata (Matsumura), Empoascanara indica (Datta), Exitianus indicus (Distant), dan 1 spesies wereng batang (Hemiptera: Delphacidae), yaitu Sogatella furcifera (Horváth). Deteksi fitoplasma pada tanaman dan wereng dilakukan dengan nested-PCR, sikuensing, dan analisis DNA sikuen. Nested-PCR berhasil mengamplifikasi DNA target, yaitu gen 16S rRNA fitoplasma dari tanaman dan wereng. Analisis identitas matriks sikuen fitoplasma asal tanaman wortel di Bogor menunjukkan homologi tertinggi dengan fitoplasma dari grup 16SrII, yaitu Peanut witches’-broom phytoplasma; sedangkan sampel asal Bandung menunjukkan homologi tertinggi dengan fitoplasma dari grup 16SrI, yaitu Ca. Phytoplasma asteris dan Tomato big bud phytoplasma. Analisis identitas matriks sikuen fitoplasma asal wereng di Bogor (B. incisa, S. furcifera) menunjukkan homologi tertinggi dengan fitoplasma dari grup 16SrII, yaitu Cactus witches’-broom phytoplasma; sedangkan fitoplasma asal C. bipunctata dari Bogor menunjukkan homologi tertinggi dengan fitoplasma grup 16SrI, yaitu Ca. Phytoplasma asteris. Tulisan ini merupakan laporan pertama fitoplasma yang menginfeksi tanaman wortel di Bogor dan Bandung.
The Potential of a Low-Cost Thermal Camera for Early Detection of Temperature Changes in Virus-Infected Chili Plants Asmar Hasan; Widodo Widodo; Kikin Hamzah Mutaqin; Muhammad Taufik; Sri Hendrastuti Hidayat
Journal of ICT Research and Applications Vol. 17 No. 1 (2023)
Publisher : DRPM - ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/itbj.ict.res.appl.2023.17.1.2

Abstract

One effect of viral infection on plant physiology is increased stomata closure so that the transpiration rate is low, which in turn causes an increase in leaf temperature. Changes in plant leaf temperature can be measured by thermography using high-resolution thermal cameras. The results can be used as an indicator of virus infection, even before the appearance of visible symptoms. However, the higher the sensor resolution of the thermal camera, the more expensive it is, which is an obstacle in developing the method more widely. This article describes the potential of thermography in detecting Tobacco mosaic virus infection in chili-pepper plants using a low-cost camera. A FLIR C2 camera was used to record images of plants in two treatment groups, non-inoculated (V0) and virus-inoculated plants (V1). Significantly, V1 had a lower temperature at 8 and 12 days after inoculation (dai) than those of V0, but their temperature was higher than V0 before symptoms were visible, i.e., at 17 dai. Thermography using low-cost thermal cameras has potency to detect early viral infection at 8 dai with accuracy levels (AUC) of 80.0% and 86.5% based on k-Nearest Neighbors and Naïve Bayes classifiers, respectively.
Potensi dan Mekanisme Yeast-Like Fungus Pseudozyma dalam Mengendalikan Antraknosa pada Cabai Sri Hartati; Suryo Wiyono; Sri Hendrastuti Hidayat; Meity Suradji Sinaga
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 7 No 1 (2023): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v7i1.275

Abstract

Application of biocontrol agents on pre- and post-harvest commodities is a promising disease control strategy. This research was objected to study the potencies and antagonism mechanisms of eight isolates of yeast-like fungus Pseudozyma, i.e. P. hubeiensis Dmg 18 BEP, Dmg 20 DEP, Dmg 23 DEP, Dmg 27 BEP, and Dmg 32 DEP, P. shanxiensis Dmg 28 DEP, dan P. aphidis SG 25 BE and SG 53 BE in controlling Colletotrichum acutatum, the cause of anthracnose on chili. Those isolates of Pseudozyma were obtain from chili leaf and fruit surfaces and tissues. To study their potencies in controlling anthracnose on chili caused by C. acutatum, the eight Pseudozyma isolates were applied on chili fruit. While the study of the antagonism mechanisms was performed by antibiosis, volatile compound formation, chitinolitic activities, hyperparasitism, and ACC deaminase production tests. The results showed that all isolates of the Pseudozyma had the potencies to control anthracnose on chili caused by C. acutatum with more than 60% control levels. The mechanisms were production of volatile compounds, chitinolitic activities, and hyperparasitism. Those Pseudozyma isolates did not produce ACC deaminase.
The Relationship Between Soil Fertility and Basal Stem Rot Disease in Oil Palm Plantations Evan Purnama Ramdan; Arief Hartono; Giyanto Giyanto; Sri Hendrastuti Hidayat; Widodo Widodo
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 7 No 1 (2023): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v7i1.384

Abstract

In oil palm, Ganoderma boninense causes stem rot disease, which is often difficult to control, and soil fertility status is related to the ecology of G. boninense as a soil-borne pathogen. Therefore, this study aims to evaluate the relationship between soil fertility and stem rot disease as well as appropriate management methods to control the disease. This was carried out at the Nusantara Plantation Company's 7, Unit Kiwah Rejosari-Pematang from June 2021 to January 2022. The determination of observation blocks was carried out selectively using three blocks of land attacked by Ganoderma boninense with the same criteria for the year of planting and the same soil type. Each block consists of five plots. Each plot consisted of five sub-plots, consisting of 3 oil palms for disease severity assessment and soil sampling. The soil for each subplot was composed of 15 samples, which were analyzed for physical and chemical properties of the soil. Determination of fertility status based on the soil research manual published by the Indonesian Bogor Soil Research Center with parameters from the analysis results. The limiting factor for fertility is the cation exchange capacity of the soil, which ranges from 10.07 meq/100 g to 17.68 meq/100  g, and the C-organic content, which ranges from 0.40 to 1.15%. According to chi-square analysis, this fertility-limiting factor is related to disease severity. Therefore, management needs to be done by adding organic matter to the soil, practicing organic or inorganic fertilization, and following the principles of cultivating healthy oil palm plants.
Incidence and Molecular-Based Identification of Papaya ringspot virus Infecting Papaya in Java Farida, Naimatul; Damayanti, Tri Asmira; Efendi, Darda; Hidayat, Sri Hendrastuti
Jurnal Fitopatologi Indonesia Vol. 18 No. 1 (2022): Januari 2022
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.18.1.43-51

Abstract

Infeksi Papaya ringspot virus (PRSV) di Indonesia pertama kali dilaporkan pada pepaya di Nangroe Aceh Darussalam pada tahun 2012. Sejak itu, PRSV atau penyakit bercak bercincin pada pepaya menyebar ke beberapa daerah di Jawa, Sumatera, Bali, dan Nusa Tenggara Barat. Penelitian ini dilakukan untuk mengonfirmasi keberadaan PRSV di beberapa daerah penanaman pepaya di Jawa dan mengetahui identitas molekulernya. Metode double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) dengan antiserum spesifik PRSV digunakan untuk mendeteksi sampel dari lapangan. Insidensi PRSV hasil deteksi DAS-ELISA di Bogor, Purworejo, Kebumen, dan Bantul berturut-turut sebesar 59.1%, 51.4%, 84.2%, dan 96.2%. Identifikasi lebih lanjut dilakukan dengan reverse transcription-polymerase chain reaction menggunakan primer spesifik (PRSV326/PRSV800), dilanjutkan dengan sikuensing DNA. Fragmen DNA berukuran 475 pb berhasil diamplifikasi dari sampel lapangan dan analisis nukleotida menunjukkan bahwa semua sampel terkonfirmasi PRSV dengan homologi antarisolat berkisar antara 95.4% sampai 99.4%. Analisis filogenetika menunjukkan bahwa isolat-isolat PRSV di Jawa berada dalam satu grup yang sama dengan isolat PRSV-P pepaya dari Thailand.
Co-Authors . SUDARSONO Abdul Muin Adnan ABDUL MUNIF ALI NURMANSYAH Amelia Feryna Bulan Dini Ana Septiana Saputri Anas Dinurrohman Susila Aqlima , Aqlima, nFN Arifin Tasrif Asmar Hasan Asniwita Asniwita Astri Windia Wulandari Wulandari ATI SRI DURIAT Awang Maharijaya Ayu Kartini Parawansa Bambang S. Purwoko Bambang Sapta Purwoko Bonny Poernomo Wahyu Soekarno Bonny Poernomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Budi Tjahjono Darni Rambu D. Siala Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Diny Dinarti Dono Wahyuno Dono Wahyuno Dwi Astuti Dwi Subekti DWI SUBEKTI Dwi Wiyati Nurul Septariani Dwiwiyati Nurul Septariani Efendi, Darda Efi Toding Tondok Eliza Suryati Rusli Endang Nurhayati Endang Nurhayati Evan P. Ramdan Farida, Naimatul Fitrianingrum Kurniawati, Fitrianingrum Gede Suastika GEDE SUASTIKA GEDE SUASTIKA Gede Suastika Gede Suastika Gede Suastika Giyanto Giyanto Giyanto Hamdayanty Hamdayanty Hanif, Andini Hari Priwiratama Harwan Susetio Heriyanto Syafutra I Wayan Winasa Ifa Manzila Ifa Manzila Ika Mariska Ika Mariska Irsan Nuhantoro Ishak Manti ISHAK MANTI Isti Wulandari Jamsari Jamsari Jati Adiputra John Thomas, John Joni Hidayat, Joni Jumanto Harjosudarmo Jumanto Harjosudarmo JUMANTO HARJOSUDARMO Jumsu Trisno Kadwati Kadwati Kikin H Mutaqin KIKIN HAMZAH MUTAQIN Ladja, Fausiah T. Laksono Trisnantoro Listihani, Listihani Mawarni, Sofi Meity S Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Melinda . Meliyana Memen Surahman Miftakhurohmah Miftakhurohmah Miftakhurohmah Mimi Sutrawati Muh. Taufik Muhamad Syukur Muhammad Herman Muhammad Taufik Muhammad Taufik MUHAMMAD TAUFIK Nada, Azmi Khoirin Neni Gunaeni Niken Nur Kasim, Niken Nur Nissa Fawwaz Adilah NOOR AIDAWATI ORAWAN CHATCHAWANKAN PANICH Prabawati Hyunita Putri Puji Lestari PURNAMA HIDAYAT Purwoko, Bambang Sapto Purwono Purwono Rahayuwati, Sat RAHMI YUNIANTI Ramdan, Evan Purnama RAUF, AUNU Redy Gaswanto Redy Gaswanto, Redy Refa Yulianingsih Reymas M.R. Ruimassa Rina Rachmawati Rita Noveriza Rita Noveriza RITA NOVERIZA Rita Noveriza Rizqiyah, Sakinah Inayatur Rokhana Faizah Roy Ibrahim RR. Ella Evrita Hestiandari Rusmilah Suseno RUSMILAH SUSENO Rustiani, Ummu S. Saiful Akhyar Lubis Sari Nurulita Sari Nurulita Sari, Rahmah Dian Sarsidi S astrosumarjo Sarsidi Sastrosumarjo Satya Nugroho Sayekti, Tri Wahono Dyah Ayu Sherli Anggraini Sientje Mandang Sumaraw Siregar, Ivan Arif Rachman Martua Siti Hafsah Siti Shofiya Nasution Slamet Susanto Sobir Sobir Soemartono Sosromarsono Soemartono Sosromarsono Sri Hartati Sri Hartati Sri Hartati Sri Sulandari Sri Sulandari Sri Sulandari Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati SRIANI SUJIPRIHATI Sugeng Santoso Supramana Suryo Wiyono Susanti Mugi Lestari Tega Kintasari Titiek YULIANTI TRI ASMIRA DAMAYANTI TRI JOKO SANTOSO Trikoesoemaningtyas Trimuri Habazar TRIMURTI HABAZAR Triyani Dumaria Tutik Harmiyati, Tutik Ummu S. Rustiani Ummu Salamah Rustiani Utomo Kartosuwondo UTOMO KARTOSUWONDO Utomo Kartosuwondo UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO Vinsen Willi Wardhana Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Willing Bagariang Zahratul Millah