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Identity and Sequence Diversity of Begomovirus Associated with Yellow Leaf Curl Disease of Tomato in Indonesia TRI JOKO SANTOSO; SRI HENDRASTUTI HIDAYAT; ATI SRI DURIAT; MUHAMMAD HERMAN; . SUDARSONO
Microbiology Indonesia Vol. 2 No. 1 (2008): April 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.464 KB) | DOI: 10.5454/mi.2.1.1

Abstract

Infection of tomato by Begomovirus is known to cause serious disease and yield losses. Samples of tomato plants showing typical symptoms of begomovirus infection were collected from eight locations in Java and Sumatra. Amplification of a putative AV1 gene was performed using AV1 specific primers for Geminivirus, total nucleic acid isolated from tomato samples exhibiting leaf curl disease as the template, and the PCR technique. Direct sequencing of PCR product was carried out, followed by nucleotide and predicted amino acid sequence analysis using the BLAST program. Positive results were obtained, the PCR amplification proved that diseased tomato samples collected from eight locations in Java and Sumatra were infected with Begomovirus. When nucleic acid and amino acid sequences of the eight isolates were compared to other begomovirus’s sequences present in the GenBank it was found that the isolates determined in this research were Indonesian isolates of AYVV. Further phylogenetic analysis of eight Begomovirus isolates identified in this study indicated they belonged into two different clades. Results of this research also suggest that the existence of Begomovirus genetic diversity in various regions in Indonesia needs further investigation. Moreover, the prevalence of distinct Begomovirus species or isolates also need investigation.
Detection and Sequence Diversity of Begomovirus Associated with Yellow Leaf Curl Disease of Pepper (Capsicum annuum) in West Sumatra, Indonesia JUMSU TRISNO; SRI HENDRASTUTI HIDAYAT; TRIMURTI HABAZAR; ISHAK MANTI; . JAMSARI
Microbiology Indonesia Vol. 3 No. 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.352 KB) | DOI: 10.5454/mi.3.2.2

Abstract

Yellow leaf curl disease of pepper has become an emerging important disease in West Sumatra since early 2000. Several attempts have been made, including disease survey and detection, in order to identify the causal agent of the disease. Pepper (Capsicum annuum) plants showing yellow leaf curl from West Sumatra were analyzed for presence of Begomovirus employing Polymerase Chain Reaction (PCR) with degenerate primers pAL1v 1978 and pARc 715. A DNA fragment of 1.6 kb was successfully amplified and subjected to direct sequencing. A stem loop region was found in the nucleotide sequence obtained, which contains the conserved nucleotide signature sequence TAATATTAC present in begomoviruses. Based on the stem loop region comparisons and phylogenetic analysis, the virus isolates from West Sumatra showed the closest relationship to Pepper yellow leaf curl Indonesia virus (PYLVIV) and Tomato yellow leaf curl Indonesia virus (TYLCIV). The sequence was different from other Asia Begomoviruses reported earlier. These isolates were divided into three groups which were tentatively called Pepper yellow leaf curl Indonesia virus-West Sumatra-[group 1], -[group 2] and -[group 3] {PYLCIV-WS-[group1], -[group2], and -[group3]}.
TIGA SPESIES PERONOSCLEROSPORA PENYEBAB PENYAKIT BULAI JAGUNG DI INDONESIA Ummu S. Rustiani; Meity S. Sinaga; Sri Hendrastuti Hidayat; Suryo Wiyono
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1860

Abstract

Downy mildew is very detrimental disease of maize production in Indonesia. Adequate information regarding the identification key based on morphological and morphometric characteristic of the causal fungi of maize downy mildew in Indonesia is limited. Study for detection and identification of morphological, morphometric, and moleculer base is urgently required. Artificial sporulation induction method performed to obtain the morphology of the fungus as a whole.The fungi were morphologically identified as symptomatic maize downy mildew collected from 13 provinces in Indonesia.Three species, namely P. maydis, P.sorghi, and P. philippinensis were identified based on the shape,size, and conidial cell wall thickness, size and number of branching coniodiophores, and long sterigmata.Confirmation by PCR succesfully amplified target DNA of P. maydis, P. sorghi, and P. philippinensis. The identification key of third species of Peronosclerospora accurately to be used in identification of Peronosclerospora causes downy mildew of maize in Indonesia.This identification key is recommended as a method of identification of the causal downy mildew of maize in Indonesia.
IDENTIFIKASI MOLEKULER BEAN COMMON MOSAIC VIRUS YANG BERASOSIASI DENGAN PENYAKIT MOSAIK KUNING KACANG PANJANG Melinda .; Tri Asmira Damayanti; Sri Hendrastuti Hidayat
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 2 (2015): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.316 KB) | DOI: 10.23960/j.hptt.215132-140

Abstract

Molecular identification of bean common mosaic virus associated with yellow mosaic disease on yard long bean. Bean common mosaic virus (BCMV) has been reported as one of the causal agents of yellow mosaic disease on yard long bean in West Java and Central Java. Infected plants showed mosaic, yellowing, and mixture of yellow mosaic. The research was conducted to identify the diversity of BCMV associated with yellow mosaic disease based on coat protein (CP) gene sequences. Symptomatic leaf samples were collected from yard long bean growing areas in several districts in West Java (Bogor, Cirebon, Subang, and Indramayu), and several districts in Central Java (Tegal, Klaten, Solo, Yogjakarta, Sleman, and Magelang). Molecular detection using RT-PCR method was carried out by using specific primer to BCMV which will amplify the CP gene. DNA fragment, + 860 bp in size, was successfully amplified from 8 out of 13 leaf samples, i.e samples from three villages in Bogor District (Cangkurawok, Bubulak, Bojong), and five samples from District of Cirebon, Subang, Solo, Sleman, and Tegal. Sequence analysis of those DNA fragment showed that 4 isolates (Bogor-Cangkurawok, Subang, Solo and Sleman) had the highest homology to BCMV-BlC from Taiwan, whereas 2 isolates (Cirebon and Tegal) had the highest homology to BCMVNL1 from England. Further, phyllogenetic analysis revealed that those of 4 isolates were closely related to BCMV-BlC from Taiwan based on nucleotide as well as amino acid sequences; while those other 2 isolates were closely related to BCMV-NL1 from England based on nucleotide sequences but closely related to BCMV-BlC Y from China based on amino acid sequences. Phyllogenetic analysis showed that those of 6 BCMV isolates separated in two different clusters; 4 isolates (Bogor- Cangkurawok, Subang, Solo, and Sleman) in cluster 1 together with BCMV-BlC from Taiwan, while other 2 isolates (Cirebon and Tegal) in cluster 2 together with BCMV-NL1.
VIRULENSI EMPAT ISOLAT CHILLI VEINAL MOTTLE POTYVIRUS PADA TANAMAN CABAI (CAPSICUM ANNUUM L.) Ifa Manzila; Sri Hendrastuti Hidayat; Ika Mariska; Sriani Sujiprihati
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 2 (2011): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.005 KB) | DOI: 10.23960/j.hptt.211122-129

Abstract

Infection of Chilli veinal mottle potyvirus (ChiVMV) has been reported from chilli pepper growing area in Indonesia. Field observation showed wide variation of symptom development starting from mild mottle to severe mottle and vein banding. A research was conducted to study the difference of isolates of ChiVMV collected from West Java (Cikabayan), Central Java (Karadenan), South Kalimantan (Nusa Indah), and West Sumatera (Tanah Datar) based on their response on 10 genotypes of chilli peppers i.e. Jatilaba, Helem, VC246, Keriting Bogor, PBC485, Titisuper, Beauty Bell, Gelora, IPBC Tanjung, and Keriting Sumatera. Each isolates of ChiVMV was mechanically inoculated to each chilli pepper genotype, and observation was conducted every day to record incubation period, symptoms type, and disease incidence. Cikabayan and Nusa Indah isolates were able to infect all chilli pepper genotypes with the shortest incubation period of 3 days in “Tistsuper” and “Beauty Bell” genotypes; whereas Karadenan and Tanah Datar isolates only infected 4 and 6 chilli pepper genotypes, respectively. Although the 4 isolates of ChiVMV showed differences in their host range, but most of them developed similar symptoms ranging from mottle, vein banding, leaf cupping, and malformation. Based on the host response, ChiVMV isolates from Cikabayan and Nusa Indah were considered more virulent than the isolates from Karadenan and Tanah Datar.
Identifikasi Penyebab Penyakit Daun Keriting Kuning pada Tanaman Mentimun Dwiwiyati Nurul Septariani; Sri Hendrastuti Hidayat; Endang Nurhayati
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 1 (2014): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (121.026 KB) | DOI: 10.23960/j.hptt.11480-86

Abstract

ABSTRACTIdentification of the causal agent of yellow leaf curl disease on cucumbers. Yellow leaf curl disease has been reported to cause serious diseases and yield losses on tobacco, chilli pepper, and tomato plants in Java. Similar symptoms were observed recently on cucumber plants from several growing areas in West Java (Bogor), Central Java (Tegal and Sukoharjo), and Yogyakarta (Sleman). Symptom variations including mosaic, chlorotic spotting, leaf curling, blistering, vein banding, reduction and distortion of leaf and fruit were observed. Serological detection using Enzyme Linked Immunosorbent Assay (ELISA) showed infection of several viruses. Antibodies specific to Squash mosaic comovirus (SqMV), Zucchini yellow mosaic potyvirus (ZyMV), dan Cucumber mosaic cucumovirus (CMV) were reacted positively with field samples. No serological reactions were observed with antibodies to Tobacco ringspot potyvirus (TRSV) and Watermelon mosaic potyvirus (WMV). Molecular detection approach based on Polymerase Chain Reaction was undergone using universal primers for Geminivirus, pAL1v1978 and pAR1c715. DNA fragment 1600 bp in size, was successfully amplified from leaf samples originated from Tegal, Sleman, Bogor, and Sukoharjo. Further identification by nucleotide sequencing indicated that virus isolates causing yellow leaf curl disease on cucumber have highest homology (95.7% to 98.6%) with Tomato leaf curl New Delhi virus-[Cucumber:Indonesia] (AB613825) from Klaten, Central Java, Indonesia.
PEWARISAN KARAKTER KETAHANAN TERHADAP CHIVMV (CHILLI VEINAL MOTTLE VIRUS) PADA TANAMAN CABAI Zahratul Millah; Sriani Sujiprihati; Sri Hendrastuti Hidayat
Jurnal Agroekoteknologi Vol 4, No 1 (2012)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.076 KB) | DOI: 10.33512/j.agrtek.v4i1.620

Abstract

Inheritance of resistance trait to ChiVMV was studied in intraspecific capsicum population derived from a cross between two Capsicum annuum line, PBC495 as a resistance line and ICPN12#4 as a susceptible line. The resistance was assessed by diseased index (DI) and score of absorbance value at  405 nm, two weeks after inoculation. Based on t-test of F1 and F1R, it was concluded that there was no maternal effect. Nil symptoms resembling the resistant parent were identified in the progeny F2, BC1P1 (BC1 to the resistant parent) and BC1P2 (BC1 to the susceptible parent) populations. Segregation of resistance (nil DI and lower absorbance value) and susceptibility in the F2 fitted a 3:1 Mendelian ratio, indicating that resistance were controlled by a single dominant nuclear gene. Nil segregation of the trait in the test crosses in BC1P1 and a 1:1 ratio segregation in BC1P2 also confirmed the 3:1 gene segregating model as found in the F2. Heritability values of the trait were medium to high.Key words : inheritance, resistance, ChiVMV, pepper
ELIMINASI Potyvirus PENYEBAB PENYAKIT MOSAIK PADA TANAMAN NILAM DENGAN KULTUR MERISTEM APIKAL DAN PERLAKUAN AIR PANAS PADA SETEK BATANG RITA NOVERIZA; GEDE SUASTIKA; SRI HENDRASTUTI HIDAYAT; UTOMO KARTOSUWONDO
Jurnal Penelitian Tanaman Industri Vol 18, No 3 (2012): September 2012
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v18n3.2012.107-114

Abstract

ABSTRAKMinyak nilam merupakan salah satu bahan baku parfum multifungsiyang bernilai tinggi. Budidaya dan pengembangan tanaman nilamterkendala oleh serangan Potyvirus yang menyebabkan penyakit mosaik.Penelitian ini bertujuan untuk mendapatkan benih nilam bebas virusdengan metode kultur meristem apikal dan perlakuan air panas pada setekbatang. Penelitian dilaksanakan mulai Januari sampai Desember 2010 diLaboratorium Virologi Tumbuhan, Institut Pertanian Bogor dan RumahKasa Hama dan Penyakit, Balai Penelitian Tanaman Obat dan Aromatik(Balittro) di Bogor. Bahan tanaman yang digunakan adalah tiga varietasnilam (Sidikalang, Lhokseumawe, Tapak Tuan). Penelitian terdiri atas (1)Eliminasi Potyvirus pada tanaman nilam menggunakan kultur meristemapikal dan (2) Eliminasi Potyvirus pada setek batang nilam denganperlakuan air panas. Percobaan pertama disusun menggunakan rancanganacak lengkap dengan perlakuan 3 varietas nilam dan 2 tipe eksplan(meristem apikal dan batang terminal), dan diulang 10 kali. Parameteryang diamati adalah persentase pertumbuhan, waktu inisiasi, tinggi, danwarna tunas, serta persentase tanaman yang terinfeksi Potyvirus.Percobaan kedua menggunakan air panas pada tiga tingkatan suhu (50, 55,dan 60 o C) dan tingkatan waktu perendaman (10, 20, dan 30 menit).Percobaan disusun menggunakan rancangan acak lengkap dengan 10perlakuan dan 10 ulangan. Tanaman nilam dipelihara selama 8 minggu dandilakukan pengamatan tinggi setek yang tumbuh dan daun yang bergejalamosaik. Hasil penelitian menunjukkan bahwa tanaman nilam, yangdiperbanyak dari kultur meristem apikal ukuran 0,5-1 mm, menghasilkan33,3-99,9% tanaman bebas virus. Perendaman setek batang nilam di dalamair panas pada suhu 50-60 o C selama 10-30 menit tidak dapatmengeliminasi Potyvirus yang menginfeksi ketiga varietas nilam yangdiuji. Setek batang nilam varietas Tapak Tuan dan Lhokseumawe lebihtoleran terhadap air panas dibandingkan Sidikalang tetapi daya tumbuhyasemakin menurun seiring semakin lama waktu perendaman. Teknik kulturmeristem apikal berpotensi untuk menghasilkan setek nilam yang bebasvirus.Kata kunci : kultur meristem apikal, perlakuan air panas, Pogostemoncablin, PotyvirusABSTRACTPatchouli oil produced by patchouli plant is one of multifunctioningperfume’s raw materials and has high economic value. One importantconstraint during its cultivation is infection by Potyvirus causing seriousmosaic disease. This study was conducted to develop a technique toproduce virus-free cutting seeds using apical meristem culture and hotwater treatment on stem cutting. The study was carried out from January toDecember 2010 in Plant Virology Laboratory of Bogor AgriculturalUniversity and Pest and Diseases screen house of Indonesian Medicinaland Aromatic Crops Research Institute (Balittro) in Bogor. Three varietiesof patchouli plant, i.e. Sidikalang, Lhokseumawe, and Tapak Tuan, wereused in this study. The study consisted of (1) Elimination Potyvirus incuttings of patchouli through apical meristem culture and (2) EliminationPotyvirus in stem cuttings of patchouli with hot water treatment. The firstexperiment was arranged using completely randomized design withtreatments of three patchouli varieties and two explant types (apicalmeristem and stem terminal), and it was replicated 10 times. Parametersobserved were bud growth percentage, initiation time, height, and color,and also percentage of plant infected by Potyvirus. The second experimentapplied hot water at three temperature levels (50, 55, and 60 o C) andsubmersion periods (10, 20, and 30 minutes). It was arranged usingrandomized complete design, consisting of 10 treatments with 10 plantsfor each treatment. The patchouli plants were maintained for 8 weeks andobservations were made for height of growing cuttings and leaves withmosaic symptoms. The results showed that the patchouli plants propagatedfrom apical meristem culture of 0.5-1 mm in sizes yielded 33.3-99.9%virus-free plants. Submersion of patchouli stem cutting seeds in hot waterof 50-60 o C and soaking period of 10-30 minutes could not eliminated theinfecting Potyvirus on patchouli the three tested varieties. Cutting seeds ofLhokseumawe and Tapak Tuan varieties were more tolerant to hot waterthan Sidikalang one. However, their ability to grow decreased in line withlonger submersion time period. Apical meristem culture technique ispotential to produce virus-free cutting seeds of patchouli.Key words: apical meristem culture, hot water treatment, Pogostemoncablin, Potyvirus
Identitas genetik Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) dari daerah endemik penyakit kuning cabai di Indonesia bagian barat berdasarkan fragmen mitokondria sitokrom oksidase I (mtCOI) Sat Rahayuwati; Sri Hendrastuti Hidayat; Purnama Hidayat
Jurnal Entomologi Indonesia Vol 13 No 3 (2016): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (482.588 KB) | DOI: 10.5994/jei.13.3.156

Abstract

Bemisia tabaci (Gennadius) is an important horticulture pest and is know to be a vector of Geminivirus. Pepper yellow leaf curl Indonesia virus (PYLCIV) is caused by Geminivirus, know to be transmitted by B. tabaci. It became an important disease in pepper and could cause 100% yield loss. Currently there is limited information concerning the genetic of B. tabaci associated with Geminivirus in Indonesia. It is therefore important to investigate the genetic identity of B. tabaci across different population. The aim of this research was to investigate the genetic identity of B. tabaci that were collected from pepper yellow leaf curl endemic area in the western part of Indonesia. Research activities include 1) sample collection of B. tabaci from pepper yellow leaf curl endemic area in west Indonesia; 2) single extraction of B. tabaci total DNA; 3) amplification of mitochondrial Cytochrome Oxidase I gene (mtCOI) fragmen with polymerase chain reaction (PCR); 4) mtCOI sekuencing 5) phylogenetic analysis of mtCOI fragmens from west Indonesian PYLCIV compared with some B. tabaci mtCOI deposited in National Center for Biotechnology Information (NCBI). Based on mtCOI fragment analysis, B. tabaci from PYLCIV endemic area were in one group belong to Asia I clusters. Genetic identity of B. tabaci from endemic area in Western Part of Indonesia were similar with polyatomic cladogram.
Variasi morfologi puparium Bemisa tabaci (Gennadius) (Hemiptera: Aleyrodidae) pada berbagai inang dan ketinggian tempat dari daerah endemik penyakit kuning cabai di Wilayah Sundaland Sat Rahayuwati; Purnama Hidayat; Sri Hendrastuti Hidayat
Jurnal Entomologi Indonesia Vol 17 No 2 (2020): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.17.2.61

Abstract

Bemisia tabaci (Gennadius) is one of polyphagous whitefly that has been known as gemini virus vector. The identification of B. tabaci is carried out based on pupal case or puparium of red eye fouth stage. The morphological variation of B. tabaci puparium leads to the difficulties on species identification. This research was aimed to study the morphological variations of B. tabaci puparium that has been found in various host plants at various altitude. Samples of B. tabaci puparium were obtained from Sundaland endemic area of pepper yellow curl disease virus endemic areas including West Sumatra, West Java, Central Java, East Java, Bali, South Kalimantan. Slides-mounted were made from puparium and then identification was carried out to species. Observations have been conducted on B. tabaci puparial size, puparial shape, number of elongated dorsal setae, and caudal setae size. Canonical correlation analysis was applied to determine the factors that affected the puparium morphological variation. Based on the results, there were four variations of puparium: oval, oval with 1–2 indentations, oval with more than 3 indentations, and sea shell shapes. The variation observed on dorsal setae number, puparial shape, and size of B. tabaci were supposed due to the induction of trichomes on the leaves surface. Puparium variations were affected by host plants instead of altitude.
Co-Authors . SUDARSONO Abdul Muin Adnan ABDUL MUNIF ALI NURMANSYAH Amelia Feryna Bulan Dini Ana Septiana Saputri Anas Dinurrohman Susila Aqlima , Aqlima, nFN Arifin Tasrif Asmar Hasan Asniwita Asniwita Astri Windia Wulandari Wulandari ATI SRI DURIAT Awang Maharijaya Ayu Kartini Parawansa Bambang S. Purwoko Bambang Sapta Purwoko Bonny Poernomo Wahyu Soekarno Bonny Poernomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Budi Tjahjono Darni Rambu D. Siala Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Diny Dinarti Dono Wahyuno Dono Wahyuno Dwi Astuti Dwi Subekti DWI SUBEKTI Dwi Wiyati Nurul Septariani Dwiwiyati Nurul Septariani Efendi, Darda Efi Toding Tondok Eliza Suryati Rusli Endang Nurhayati Endang Nurhayati Evan P. Ramdan Farida, Naimatul Fitrianingrum Kurniawati, Fitrianingrum Gede Suastika GEDE SUASTIKA GEDE SUASTIKA Gede Suastika Gede Suastika Gede Suastika Giyanto Giyanto Giyanto Hamdayanty Hamdayanty Hanif, Andini Hari Priwiratama Harwan Susetio Heriyanto Syafutra I Wayan Winasa Ifa Manzila Ifa Manzila Ika Mariska Ika Mariska Irsan Nuhantoro Ishak Manti ISHAK MANTI Isti Wulandari Jamsari Jamsari Jati Adiputra John Thomas, John Joni Hidayat, Joni Jumanto Harjosudarmo Jumanto Harjosudarmo JUMANTO HARJOSUDARMO Jumsu Trisno Kadwati Kadwati Kikin H Mutaqin KIKIN HAMZAH MUTAQIN Ladja, Fausiah T. Laksono Trisnantoro Listihani, Listihani Mawarni, Sofi Meity S Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Melinda . Meliyana Memen Surahman Miftakhurohmah Miftakhurohmah Miftakhurohmah Mimi Sutrawati Muh. Taufik Muhamad Syukur Muhammad Herman Muhammad Taufik Muhammad Taufik MUHAMMAD TAUFIK Nada, Azmi Khoirin Neni Gunaeni Niken Nur Kasim, Niken Nur Nissa Fawwaz Adilah NOOR AIDAWATI ORAWAN CHATCHAWANKAN PANICH Prabawati Hyunita Putri Puji Lestari PURNAMA HIDAYAT Purwoko, Bambang Sapto Purwono Purwono Rahayuwati, Sat RAHMI YUNIANTI Ramdan, Evan Purnama RAUF, AUNU Redy Gaswanto Redy Gaswanto, Redy Refa Yulianingsih Reymas M.R. Ruimassa Rina Rachmawati Rita Noveriza Rita Noveriza RITA NOVERIZA Rita Noveriza Rizqiyah, Sakinah Inayatur Rokhana Faizah Roy Ibrahim RR. Ella Evrita Hestiandari Rusmilah Suseno RUSMILAH SUSENO Rustiani, Ummu S. Saiful Akhyar Lubis Sari Nurulita Sari Nurulita Sari, Rahmah Dian Sarsidi S astrosumarjo Sarsidi Sastrosumarjo Satya Nugroho Sayekti, Tri Wahono Dyah Ayu Sherli Anggraini Sientje Mandang Sumaraw Siregar, Ivan Arif Rachman Martua Siti Hafsah Siti Shofiya Nasution Slamet Susanto Sobir Sobir Soemartono Sosromarsono Soemartono Sosromarsono Sri Hartati Sri Hartati Sri Hartati Sri Sulandari Sri Sulandari Sri Sulandari Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati SRIANI SUJIPRIHATI Sugeng Santoso Supramana Suryo Wiyono Susanti Mugi Lestari Tega Kintasari Titiek YULIANTI TRI ASMIRA DAMAYANTI TRI JOKO SANTOSO Trikoesoemaningtyas Trimuri Habazar TRIMURTI HABAZAR Triyani Dumaria Tutik Harmiyati, Tutik Ummu S. Rustiani Ummu Salamah Rustiani Utomo Kartosuwondo UTOMO KARTOSUWONDO Utomo Kartosuwondo UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO Vinsen Willi Wardhana Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Willing Bagariang Zahratul Millah