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All Journal Jurnal Sain Veteriner Jurnal Veteriner Media Kedokteran Hewan Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Aceh Journal of Animal Science Jurnal Medik Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary
Endang Suprihati
Division Of Veterinary Parasitology, Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Journal : Jurnal Veteriner

 1 
Deteksi Cryptosporidium canis pada Anjing di Kota Surabaya (CRYPTOSPORIDIUM CANIS DETECTION IN DOGS IN THE CITY OF SURABAYA) Romy Muhammad Dary Mufa; Nunuk Dyah Retno Lastuti; Fedik Abdul Rantam; Lucia Tri Suwanti; Endang Suprihati; Didik Handijatno; Mufasirin Mufasirin
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cryptosporidiosis is a disease caused by Cryptosporidium spp. protozoan parasites and are zoonotic. Cryptosporidium canis is the main species that infects dogs. Transmission of C. canis in dogs to humans is possible. This study aims to detect microscopic C. canis infection based on morphology and molecularity using the Polymerase Chain Reaction (PCR) in dogs in Surabaya City. A total of 80 diarrhea dog feces samples were taken from Animal Hospitals and animal clinics in several areas in the Surabaya City, then added potassium dichromate and stored at 4OC. Detection was made of the presence of Cryptosporidium spp. oocysts microscopically which is then confirmed by molecular examination using the PCR method. The results showed 40 positive samples containing Cryptosporidium spp., oocysts, with a size of 2-6 ?m. Ten samples from the total positive sample of Cryptosporidium spp. oocysts by microscopic examination, with the PCR test there were seven positive samples of C. canis. Based on the results of the study it can be concluded that the species that causes Cryptosporidiosis in dogs in Surabaya City is C. canis. The high cases of Cryptosporidiosis in dogs can be a warning to be able to prevent Cryptosporidium spp. infections, especially in pets that have the potential as a reservoir in spreading disease.
Deteksi Molekuler Blastocystis sp. pada Babi Terinfeksi di Kabupaten Tabanan dan Badung, Provinsi Bali, Indonesia (MOLECULAR DETECTION OF BLASTOCYSTIS INFECTION IN PIGS AT TABANAN AND BADUNG DISTRICT, BALI PROVINCE, INDONESIA) Doohan Mahendra; Lucia Tri Suwanti; Nunuk Dyah Retno Lastuti; Mufasirin Mufasirin; Endang Suprihati; Wiwik Misaco Yuniarti; Ni Komang Aprilina Widisuputri
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aims to identify morphology and molecular of Blastocystis infection in pigs in the Tabanan and Badung District, Bali Province, Indonesia. 100 samples of pig stool were taken, fifty samples from the Tabanan and fifty samples from Badung District. Blastocystis sp. isolated and identified microscopically and confirmed by polymerase chain reaction (PCR) as a molecular identification method. 63% (63/100) Blastocystis is found in pig feces from these two District by microscopic detection. Positive samples were found 27 samples from Badung and 36 samples from Tabanan. Five samples were taken from each District conducted a confirmation test using the PCR method and found 60% (6/10) positive samples, two samples from Badung and four samples from Tabanan. The results of this study indicate that in healthy pigs, they also contain parasites that have the potential to spread disease to humans. Therefore, the high incidence of Blastocystis in pigs can be an alarm or warning to prevent Blastocystis infection from livestock that can potentially to be a reservoir in the spread of disease.
Deteksi Molekuler Entamoeba suis pada Babi di Rumah Potong Hewan Pegirian Surabaya Fransiska Cicilia Beka; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Koesdarto Koesdarto; Mufasirin Mufasirin; Endang Suprihati; Dyah Ayu Kurniawati
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.657 KB) | DOI: 10.19087/jveteriner.2021.22.3.383

Abstract

Penelitian ini bertujuan untuk mendeteksi E. suis pada babi yang dipotong di Rumah PotongHewan (RPH) Pegirian, Surabaya. Sebanyak 63 sampel feses babi dikoleksi dari RPH Pegirian Surabaya. Deteksi morfologis Entamoeba spp. dilakukan dengan metode apung (analisis parasitologi). Selain itu, penentuan spesies E. suis dilakukan dengan PCR menggunakan primer Esuis764 (5’-ATC AAA TCA ATT AGG CAT AAC TA-3’) dan Esuis765 (5’-AAT TAA AAC CTT ACG GCT TTA AA-3’) dan prodak PCR yang diharapkan adalah 320 bp. DNA dari sampel positif diekstraksi dengan DNAzol. PCR dilakukan pada beberapa sampel positif yang dipilih secara acak dari analisis parasitologi. Hasil penelitian menunjukkan 57,14% (36/63) sampel secara morfologis positif Entamoeba sp. Hasil penentuan spesies berdasarkan analisis molekuler menemukan bahwa protozoa yang menginveksi babi di Rumah Potong Hewan Pegirian, Surabaya adalah E. suis.
Vaksininasi Protein Ekskretori-Sekretori Toxoplasma gondii Hasil Biakan in vivo Membangkitkan Respons Imun Non Protektif (THE VACCINATION OF Toxoplasma gondii EXCRETORY-EXCRETORY PROTEINS FROM IN VIVO CULTURE ENHANCED IMMUNE RESPONSE UNABLE PROTECTIVE) Mufasirin .; Endang Suprihati
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aims of this research was to explore the immune response and protectiveness of mice whichwere vaccinated with Toxoplasma gondii excretory-secretory proteins produced from in vivo culture. A totalof 50  Balb/c strain mice were allotted  into five groups. Mice in group 1 to 3 were vaccinated with 20.7 kDa,35.3 kDa, and 100.9 kDa of the protein, respectively.  Whereas mice in group 4 were given total  excretory-secretory antigen ( total ESA5), and mice in group 5 were used as  control (PBS).  Booster  vaccinated wasconducted at two weeks following the first vaccination. Prior the challenge test, five mice were sacrificed forimmunologlobulin-G (IgG) analysis. The analysis of IgG using enzyme linked immunosorbent assay (ELISA).Challenge test used 1x103 T. gondii tachyzoites which were given to the animals   intraperitoneally.  Theresults showed that vaccination using  T.gondii excretory-secretory proteins as well as T. gondii total ESAscould enhanced immune response as detected by the markedly  presence of T. gondii  IgG.  However, thiswas unable to protect against T. gondii RH strain infection.
Co-Authors Adi Sofyan Ansori, Muhammad Afiyah Rahmadani Agnes Theresia Soelih Estoepangestie Akbar Wijaya Putra Purnama Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Alasrorik, Muhammad Hizbulloh Aldin Akbar Rahmatullah Amara Lintang Pagati Amelia Dwita Safitri Anam Al Arif Anggreani Desi Ramadhani Rahajeng Arif Pratiwi Arimbi Arimbi Ayu, Zerlinda Dyah Azizah Bilqis Nurkarimah Azwani, Nabila Azzahra, Farah Nabila Boedi Setiawan Cindy Ercha Aulia Putri Dadik Rahardjo, Dadik Dadik Raharjo David Mohamad Qadafi Dhio Asmaydo Dian Ayu Permatasari Dian Ayu Permatasari Didik Handijatno Doohan Mahendra DWI PUTRI RAHMAWATI Dyah Ayu Kurniawati Dyah Ayu Kurniawati Ellza Agatha Damayanti Emy Koestanti Sabdoningrum Eny Coolfina Simarmata Erma Safitri Fadila Zikri Amanda Faisal Fikri Fatmawati, Mira Fatmawati Fedik Abdul Rantam Firman Hadi Fanani Fransiska Cicilia Beka Ghifari Lutfi Fauzi Hana Eliyani Hani Plumeriastuti Hartono Hartono Hayuning Nurrodhiya Heni Puspitasari Imam Mustofa Indasari, Elly Nur Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Iwan Sahrial Hamid Jihaan Haajidah Khairullah, Aswin Rafif Kiki Amalia Rama Koesdarto Koesdarto Kuncoro Puguh Santoso Kurniawan, Muhammad 'Ahdi Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Kusriningrum Rochiman Legowo, Djoko Lianny Nangoi Lilik Maslachah Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Marchelia Arifiandani Masdiana C Padaga Maudika Pamela Fiorenza Meta Aprilia Meyreta Doti Alcaterana Mirni Lamid Mochamad Lazuardi Mohammad Sukmanadi Moses, Ikechukwu Benjamin Mufasirin Muhammad Fahmi Abdillah Muhammad Hambal Mustofa Helmi Effendi Nenny Harijani Ni Komang Aprilina Widisuputri Nidom, Chairul Anwar Niluh Selly Frantika Nining Sari Virgandina Vinola Nizar Bachrudin Prihandono Nunuk D.R. Lastuti Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nurin Ardhiani Panjaitan, Cahaya Poedji Hastutiek Praja, Ratih Novita Pratama, Bima Putra Pratama, Ponasari Galuh Pratiwi, Arif Prawasa, Ramadhiniyanti Putri Alif Pudji Srianto Puput, Sesa Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Ratna Damayanti Retno Wulansari Riwu, Katty Hendriana Priscilia Rizki, Andaru Rochmah Kurnijasanti Romy Muhammad Dary Mufa Sandy, Berliana Dwi Nandita Sarmanu, Sarmanu Setiawan Koesdarto Setiawan Kusdarto Soeharsono Soeharsono Sri Mumpuni Sosiawati Sri Pantja Madyawati, Sri Pantja Sunarso, Agus Supranianondo, Koesnoto Suzanita Utama Tjuk Imam Restiadi Trilas Sardjito Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widi Nugroho Widodo, Oky Setyo Wiwik Misaco Yuniarti Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad