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Journal : Jurnal AgroBiogen

Interaksi Genetik x Lingkungan dan Variabilitas Genetik Galur Gandum Introduksi (Triticum aestivum L.) di Agroekosistem Tropika Nur, Amin; Azrai, Muh.; Trikoesoemaningtyas, Trikoesoemaningtyas
Jurnal AgroBiogen Vol 10, No 3 (2014): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n3.2014.p93-100

Abstract

Thefocus of wheat research in Indonesia is to obtained newpotential wheat lines that are adapted to low-mid elevationand heat tolerant. This study was aimed to obtaininformation on the effect of interaction of season x line xlocation and genetic variability of wheat lines in tropicalagroecosystem. This study was conducted at the Seameo-Biotrop (<400 masl) and Indonesian Ornamental CropsResearch Institute-Cipanas (>1,000 masl) experimental fieldfor two seasons. The results showed that there was an effectof interaction of season x line x location on plant height,days to flowering, number of spikelet and floret, seed/headweight, rate of grain filling, yield, flag leaf width, stomatadensity, chlorophyll b, and leaf greenness. Meanwhileseveral characters were only influenced by the interaction ofline x location, they were yield component characters, ie.empty floret percentage, number of seed/ head, 1,000 seedweight, number of head/m2 and seed/plant weight. Sevencharacters were not influenced by interaction of neitherseason x line x environment nor line x location, they werenumber of productive tillers, head length, number ofseed/head, chlorophyll a, ratio of chlorophyll a/b, totalchlorophyll, and leaf thickness. The characters that havehigh heritability and wide genetic variability for the twoanalysis models were the number of spikelet.
Nucleotide Variations of Gamma Ray-Irradiated Chili Pepper Mutant Genotypes Based on Gene-Specific Primers Related to Fruit Size Character Nugroho, Kristianto; Trikoesoemaningtyas, Trikoesoemaningtyas; Syukur, Muhamad; Lestari, Puji
Jurnal AgroBiogen Vol 17, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n2.2021.p83-94

Abstract

Fruit size is a quantitative character controlled by several genes and mutations in those genes could lead to phenotypic changes in fruits. Single nucleotide polymorphism (SNP) is a molecular marker that could be used to detect mutation in genes at the nucleotide level that could not be done by conventional markers. The aim of this study was to analyze nucleotide variations, primarily SNP, in mutant chili pepper genotypes using gene-specific primers related to fruit size characters to measure the genetic changes occured, identify the SNPs that were associated with fruit size character, and analyze the phylogenetic among the mutant genotypes. Sanger sequencing was conducted on six mutant[K21]  chili pepper genotypes from M2 generation and Lingga as origin variety using five pairs of newly designed gene-specific primers corresponding to fruit size characters. A total of 41 base changes were identified in the gamma ray-irradiated chili pepper mutants, consisting of 22 insertions/deletions (indels) and 19 SNPs. As many as 17 nonsynonymous SNPs which caused amino acid changes, in contrast to the other 2 synonymous SNPs, were found in these mutant genotypes. Association analysis between identified SNPs to fruit length and diameter showed nonsignificant association, therefore the functional marker related to targeted gene could not be developed. The genotype L269 showed a separation from Lingga in dendrogram reflecting a large number of genetic changes, showed its potential as a new germplasm in development of improved variety in the future.
Characterization of Donor Genome Segments of BC2 and BC4 Way Rarem x Oryzica Llanos-5 Progenies Detected by SNP Markers Wening Enggarini; Surjono H. Sudjahjo; Trikoesoemaningtyas Trikoesoemaningtyas; Sriani Sujiprihati; Utut Widyastuti; Kurniawan R. Trijatmiko; Sugiono Moeljopawiro; Masdiar Bustamam; Casiana V. Cruz
Jurnal AgroBiogen Vol 8, No 1 (2012): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n1.2012.p1-7

Abstract

Plant breedersmake a succession of backcrosses to introgress a characterfrom a donor parent into genomic background of a recurrentparent. In several backcrossing, the proportion of a genometends to return almost fully to recurrent parent, except thesmall donor genome segment harboring the character ofinterest. The estimation of the proportion donor segmentthrough backcross generations has been analyzedtheoretically using complex mathematical simulations. Inthis study, the proportion of donor introgression segmentswere directly analyzed in advanced backcross populations,BC2F7 and BC4F2. The analysis was done by using a set ofsingle nucleotide polymorphism (SNP) markers covering theentire rice genome. Of the 384 SNP markers we found 124markers which provide polymorphism between recurrentparent, Way Rarem and Oryzica Llanos-5 as donor parent.But only 55 SNP markers could detect Oryzica Llanos-5alleles in BC2F7 and BC4F2 progenies. The result of thisanalysis demonstrated that the average of donor segmentnumber was 14.5 in BC2F7 and 12.3 in BC4F2. It was reduced15% from BC2F7 to BC4F2. The average of donor segmentlength was 31.2 cM (centiMorgan) in BC2F7 and 8.79 cM inBC4F2. It was decreased 72% during twice backcrossing. Theaverage of donor genome size was 343.95 cM in BC2F7 and71.35 cM in BC4F2, which means there was 79% decreasefrom BC2F7 to BC4F2. These results offered a simple methodto describe the proportion of target genome segment fromdonor parent. It was required as one of the main selectioncriteria in backcross programs.
Co-Authors , Kisman , Rusdiansyah , Suwarno , Syafruddin . Giyanto . SUWARNO A. Hairmansis A. Hairmansis Abraham Talahaturuson, Abraham ADEEL ABDUL KARIM ALTUHAISH Agus Zainudin AHMAD JUNAEDI Aksarah Pas, Aris Amin Nur Angelita Puji Lestari ANGELITA PUJI LESTARI Anna Juliarti Anwar Anwar - Anwar Anwar Arfriani Maifizar Ari Fiki Aris Hairmansis Arvita Netti Sihaloho, Arvita Netti Asep Supriyanto Azis Rifianto Azrai, Muh. Azrai, Muh. Bambang S. Purwoko Bambang S. Purwoko Bambang Sapta Purwoko Basri Carolina Evelyn Casiana V. Cruz Cut Nurul Fahmi Cut Nya Dhin Cut Nya’dhin Cut Tia Mardi Danner Sagala Dasumiati Desta Wirnas Dewi Andriani Diana Sofia Hanafiah Didy Sopandie DWI ANDREAS SANTOSA Dwi Astuti Edi Santosa Edi Santosa Efendi, Darda Ekasari Putri, Nurwanita Eny Widajati Faqih Udin Fuady Giyanto Giyanto Godlif Sianipar Gunawan Gunawan Gunawan Gunawan HAJRIAL ASWIDINNOOR Halimatus Syahdia Hasibuan Herdhani, Mira Heri Junaedi Homma, and Koki Human, dan Soeranto Ilyas Ilyas Imam Widodo Indra Gunawan Irdika Mansur Irdika Mansur Iskandar Lubis Isnaini, Isnaini Iswari S. Dewi Iswari Saraswati Dewi Jaisyurahman, Usamah Jenuri Joko Prasetiyono Karlin Agustina Kartika Ning Tyas Khairul Aswadi Khairunnisa Lubis Kikin H Mutaqin Kikin H. Mutaqien Koki Homma Kristianto Nugroho KRISTIANTO NUGROHO, KRISTIANTO Kurniawan R. Trijatmiko La Muhuria Latifa Dinar Rahmani Hakim Leiwakabessy, Christoffol Lestari, Puji Limbongan, Yusuf La’lang Loso Judijanto Lubis, Khairunnisa M A Chozin M. Chalis M. Ikhsan Mahpuzah, Marfiatun Maksalmina Maksalmina Mardhiyyah, Rodhiyatan Mariati MR Maryono, Marina Yuniawati Masdiar Bustamam Mayang Sari Mayasari Yamin, Mayasari Meity Suradji Sinaga Meity Suradji Sinaga Meliala, Merry Gloria Memen Surahman Miftahudin . Momongan, Jorex Daniel Mudinillah, Adam Muhamad Saleh Muhamad Syukur Muhammad Arif Nasution Muhammad Arif Yudiarto Muhammad Junaidi Muhammad Syukur Mujiburrahman Mujiburrahman Munarti Munif Ghulamahdi Muttaqin, Kikin Hamzah Nasruddin Nasruddin Neni Rohaeni Ni Made Armini Wiendi Nirwani Jumala Nirwani Jumala Nora Fitria Nugroho, Kristianto Nur, Amin Nur, Amin Nurheni Wijayanto Nurul Hidayatun Nurul Khumaida Nurya Yuniyati Peni Lestari Permana, Fithri Angelia Pratama, Muhammad Antony Jefri PUJI LESTARI Puji Lestari Putri Andini Mandasari RAGAPADMI PURNAMANINGSIH Rahmah Johar Rani Rahmayanti Ratna Mutia Rini Hermanasari Rini Hermanasari Rini, Erin Puspita Roedhy Poerwanto RUBIYO RUBIYO RUBIYO, RUBIYO Ryandi Ferdiannur Usman S. Human S. Human Saniaty, Arina Saragih, Ery Leonardo Satya Nugroho Shiraiwa, Tatsuhiko Sihono Sihono Sinaga, Parlin H. Sintho Wahyuning Ardie Siregar, Valdi Muhamad Rafiansyah Siti Marwiyah Sobir Sobir Soeranto Human Soeranto Human Soeranto, Soeranto SRI HENDRASTUTI HIDAYAT Sri Suhesti Sriani Sujiprihati Subagio, Herman SUDARSONO SUDARSONO SUDARSONO, SUDARSONO Sudirman Yahya Sudirman Yahya Sudirman Yahya - Sugiono Moeljopawiro Sumiati, dan Sungkono Sungkono Sungkono Sungkono Supijatno Surjono H. Sudjahjo Surjono Hadi Sutjahjo Suryo Wiyono Sustiprajitno, Sustiprajitno Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno Syamsiar, Syamsiar Syarifah Iis Aisyah Tata Suryana , Aep Tatsuhiko Shiraiwa TRI JOKO SANTOSO Tri Lestari Tuti Rahmi Untung Susanto Usamah Jaisyurahman Usman Effendi Utut Widyastuti W. Puspitasari WAGE RATNA ROHAENI Wage Ratna Rohaeni Wage Ratna Rohaeni Wahyu Widiyono Wening Enggarini Wibawa, Rentang Fajar Cakra Widowati, Sartika Wijaya Murti Indriatama Wijaya Murti Indriatama Willy Bayuardi Suwarno Yudiwanti Wahyu E. Kusumo Yusuf L. Limbongan Zainuddin Zainuddin