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Journal : Journal of Tropical Crop Science

Application of Cytokinin and Mycorrhiza to Increase Production and Quality of Pineapple Seedlings from Crown Leaf Bud Cuttings Putri, Dirgahani; Suhartanto, M. Rahmad; Widajati, Eny
Journal of Tropical Crop Science Vol 7 No 01 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.091 KB) | DOI: 10.29244/jtcs.7.01.15-21

Abstract

The pineapple vegetative propagation method using crown leaf bud cuttings has potential to produce seedlings of greater uniformity and in larger quantities than other methods. This study, aimed to explore the potential eff ects of concentration and frequency of Benzylaminopurine (BAP) application for increasing the production and quality of pineapple seedlings from crown leaf bud cutting propagation. The experiment used a randomized complete block design (RCBD) with two factors. The fi rst factor was the concentration of BAP (0, 200, 400, 600, 800 ppm) and the second factor was the frequency of BAP application (1, 2, 3 times). Furthermore, this study investigated the eff ects of applying mycorrhizae to accelerate seedling growth and improve seedling quality during production from crown leaf bud cutting propagation. The experiment used a RCBD with one factor (dose of mycorrhiza: 0, 50, 100, 150, 200 spores). The results indicated that the eff ects of concentration and frequency of BAP application and their interaction increased seed production as determined by sprout cuttings at 5 weeks after planting by applying 600 ppm 2 times. Generally, with increasing concentration and frequency of BAP application, leaf chlorophyll content was reduced. However, the application mycorrhiza positively infl uenced the production of seedlings from pineapple crown bud cuttings and improved seedling quality as measured by root dry weight, chlorophyll content, root infection, and phosphatase enzyme activity.
Determination of Seed Physiological Maturity and Invigoration using Plasma-Activated Water and Ultrafine Bubble Water on Okra Seeds Nur Syam Ismaniza A., Siti Nur Syam Ismaniza A.; Widajati, Eny; Qadir , Abdul; Purwanto, Y. Aris
Journal of Tropical Crop Science Vol. 11 No. 03 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.03.217-228

Abstract

Seeds physical and physiological qualities are primarily determined by seed physiological maturity. Seed enhancement technology has developed rapidly, including using ultrafine bubbles (UFB) and plasma-activated water (PAW) to increase seed viability. This study aimed to determine seed physiology maturity on okra seed to get the optimal harvesting period seeds and to examine the most effective seed enhancement plasma-activated water and ultrafine-bubble Water. The first experiment was arranged using completely randomized design with fruit maturity as the single factor, harvested at 3, 4, 5, or 6 weeks after anthesis (WAA), with three replications. The second experiment used factorial completely randomized design; the first factor is seed lots with different storage times (fresh seed and one-year storage seed), and the second factor seed invigoration using UFB (20 ppm dissolved oxygen) and PAW (10-, 20-, and 30-min exposure) as the second factor. Untreated seeds were used as control. Fifty seeds were used in each treatment. Okra seed physiological maturity was reached at 5 WAA based on seed dry weight, germination capacity, seedling vigor, and speed of germination. The pod color at 5 WAA is olive brown, and seed testa has dark greyish purple. Plasma-activated water at 10 minutes exposure increased the vigor index of seed lots stored for one year by 86% and PAW20 by 87%. Plasma activated water at 10-, 20- and 30-minutes exposures significantly increased the seedling vigor index and germination speed of fresh seeds harvested at 5 WAA.
Protocol Development for Assessing Seed Moisture Content and Germination Testing in Amorphophallus muelleri Blume Fadhilah, Siti; Widajati, Eny; Ilyas, Satriyas; Palupi, Endah Retno; Qadir, Abdul
Journal of Tropical Crop Science Vol. 12 No. 01 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.01.132-144

Abstract

Seed quality testing involves the evaluation of germination capacity under a standardized moisture content. However, limited reports have been found for seed testing methodology, especially for Amorphophallus muelleri. Standardizing the seed testing method for A. muelleri is essential to ensure repeatability, reproducibility, and reliable seed germination results. This study aimed to develop procedures for assessing seed moisture content and germination tests. Three seed lots were used for seed moisture content determination. The necessity for seed cutting and efficacy of high-temperature oven methods (133°C for 4, 5, and 6 h) were compared to low-temperature oven methods (103°C for 17 h). Five lots of seeds of different ages were used for the seed germination test using the between-paper method in a constant temperature germinator of 25 and 30 °C. There was no significant difference in the moisture content between the uncut/whole and the seeds cut. The high temperature of 133°C for four hours is an alternative to the low temperature. Germination rates at 25 and 30°C had no significant difference, i.e., 84 to 97% and 83 to 99%, respectively. However, at 30°C, it was shorter to 50% germination (T50) and germination period. Preheating the seeds for four weeks using the dry heat method shortened the seed germination time from 57 to 37 days. Seed lot exposed to dry heat had half the time required to reach T50, or 16.4 to 31.9 days after treatment, with a germination period of 37 to 52 days. The first count of germination at 25°C without preheating was 47 days after planting, and the final count was 73 days after planting, whereas at 30OC, it was 37 and 57 days after planting. The first and final count for germination with preheating treatment at both temperatures was 20 DAP and 37 DAP.
Cytological Deterioration of Bitter Gourd (Momordica charantia L.) Pollen During Storage and Its Impact on Effectiveness for Seed Production Kusumastuti, Hamiddah Intan; Widajati, Eny; Palupi, Endah Retno
Journal of Tropical Crop Science Vol. 12 No. 02 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.02.303-313

Abstract

Pollen storage plays an important role in the hybrid seed production of bitter gourd, but a primary challenge is the rapid decline in pollen viability. This research aimed to investigate the mechanisms of bitter gourd pollen deterioration during storage and to assess the effectiveness of stored pollen for hybrid seed production. The study was conducted at the Leuwikopo Research Station, IPB University, from September 2018 to June 2021. Bitter gourd pollen was collected at anthesis and stored in a deep freezer at -21±3ºC. Pollen viability was observed at 0, 2, 4, 6, 8, 10, and 12 weeks after storage (WAS), while pollen ultrastructure was examined at 0, 4, 8, and 12 WAS using a transmission electron microscope. The stored pollen was then used for pollination, observations were made on fruit set, seed set, and seed quality. The viability of bitter gourd pollen declined rapidly following dehydration before storage. The declining pollen viability during storage was due to the degeneration of the intine, followed by mitochondrial deterioration. The cristae became disorganised, leading to the dissolution of the cristae and outer membrane of mitochondria. Storing the pollen in a deep freezer (-21±3ºC) after dehydration did not prevent a further decline in pollen viability. Bitter gourd pollen stored for more than 2 weeks at -21±3ºC is ineffective for seed production. Cucurbit pollen remains viable for only two weeks; thus, fresh pollen is more practical for hybrid seed production. Development of more effective pollen storage methods is necessary to prolong pollen viability and enhance breeding efficiency. 
Development of Rapid Viability Test Using Urine Sugar Analysis Paper for Peanut (Arachis hypogaea L.) Seeds Mubarak, Farahdina; Suhartanto, Mohamad Rahmad; Widajati, Eny
Journal of Tropical Crop Science Vol. 12 No. 02 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.02.378-387

Abstract

Seed testing is important for determining seed quality. Seed testing must be easy, quick, and accurate. This study aimed to develop a rapid method for assessing peanut seed quality using Urine Sugar Analysis Paper (USAP). USAP is commonly used for detecting human urinary glucose and is currently being developed for evaluating seed quality. Three experiments were conducted. Experiment 1 investigated the most effective seed-cutting size (whole, halved, and seeds cut into six pieces) and soaking periods (0, 3, 6, and 9 hours) based on glucose and protein leakage, as well as specific gravity of the soaking water. The optimal method identified in Experiment 1 was then used to test all five seed lots in USAP Experiment 2, which aimed to evaluate the effectiveness of seed quality testing using the USAP method at various levels of seed viability. Experiment 3 investigated the correlation between USAP test results and other viability and vigor test parameters, including germination percentage, first count germination, speed of germination, and electrical conductivity. The results of experiments indicated that cutting seeds into six pieces and 6 6-hour soaking period was the most optimal. Experiment 3 showed that USAP testing correlated with viability and vigor testing across various peanut varieties. Protein and density testing using USAP were negatively correlated with germination percentage, first count germination, and germination speed parameters, but positively correlated with electrical conductivity. However, glucose leakage was not detectable using USAP.
Invigoration Increased Quality and Storability of True Seed of Shallot (Allium ascalonicum L.) Adhinugraha, Qudus Sabha; Widajati, Eny; Palupi, Endah Retno
Journal of Tropical Crop Science Vol. 9 No. 02 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.02.145-155

Abstract

Seed invigoration is a pre-planting seed treatment by balancing the seed water potential and stimulating their metabolic activities so that the seeds germinate simultaneously. In this study, invigoration treatments were applied to improve the quality of the true seeds of shallots (TSS) that had started to deteriorate. Invigoration increases the moisture content of the seeds so the seeds need to be re-dried to extend their storability. The aims of the study were to determine the best invigoration treatment and the drying method to maintain the benefit of invigoration, and to prolong TSS storability after invigoration and drying. This research was conducted at the Laboratory of Seed Quality Testing, IPB University, Indonesia, and consisted of three experiments, i.e., invigoration of deteriorating TSS to improve their viability and vigor, drying after invigoration to extend seed storability, and seed storability after invigoration and drying treatments. The first experiment was arranged in completely randomized design with one-factor (invigoration solutions), i.e. control, medium containing 3% KNO3, 50 ppm GA3, 0.5 % ZnSO4 or PEG6000 -10 bar. The second experiment was conducted using a two-factor completely randomized design, drying temperature (20˚C, 30˚C, and 40˚C), and drying duration (8 hours, 16 hours, 32 hours, and 48 hours). The third experiment was arranged in a nested design with two factors. The first factor was a seed storage condition, i.e., ambient temperature, air-conditioned room, and refrigerator. The second factor was seed packages, i.e., aluminum foil and polypropylene plastic. Non-invigorated seeds were used as control. The studies were conducted on two shallot varieties separately, “Trisula” and “Lokananta”, except in the third experiment which only used “Lokananta”. The results showed that invigoration in 3 % KNO3 and 50 ppm GA3 effectively improved the vigor of deteriorating TSS. Drying the seeds at 40˚C for 8 hours was the most effective method to reduce seed moisture content without reducing the benefit of invigoration. Storing the invigorated TSS in an air-conditioned room with aluminum foil packaging, or in a refrigerator with aluminum foil or plastic packaging until 14 weeks maintained the benefit of invigoration. The quality of the invigorated TSS was maintained until 14 weeks in an air-conditioned room with aluminum foil packaging, or in a refrigerator with aluminum
Mechanism and Persistence Dormancy of Ground Cherry Seeds (Physalis peruviana L.) at Different Maturity Stages Pradana, Adhitya Vishnu; R. Palupi, Endah; Qadir, Abdul; Widajati, Eny
Journal of Tropical Crop Science Vol. 9 No. 03 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.03.183-192

Abstract

Ground cherry (Physalis peruviana) seeds have a period of dormancy after ripening. A study was conducted to understand the mechanism of dormancy, the duration of after ripening persistence, and to determine the effective methods to break dormancy. The study was conducted in an experimental field at Ciwidey, West Java, Indonesia, and the Seed Testing Laboratory, Faculty of Agriculture, Department of Agronomy and Horticulture, IPB University, in February to November 2020. The first study examined the effects of the storage temperatures, i.e. 20 ±2 oC and 25 ± 2oC, and the seed maturity stages, i.e. 49 days after anthesis (DAA), 58 DAA and 62 DAA. Several methods to break seed dormancy were tested, i.e. soaking seeds for 24 hours in distilled water, in 0.5% or 1% KNO3 and, 50 ppm GA3. The results showed that the seed dormancy was broken at 7 weeks after treatment, indicated by seed germination of >80%. The ABA levels of the seeds declined from week 0 to week 11, and the seed ABA and GA reached a balance from week 7 to week 11. Seeds treated with KNO3 or distilled water break dormancy after 8 weeks. The most effective treatment to break seed dormancy is the use of exogenous hormone GA3 at 50 ppm, with seed germination of >80% at week 5.
The Development of Germination Tests and Breaking Dormancy Techniques of Bitter Seeds (Andrographis paniculata (Burm. f.) Wall. ex Nees) Gundala, Belangie Tuahte; Widajati, Eny; Ilyas, Satriyas
Journal of Tropical Crop Science Vol. 10 No. 02 (2023): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.10.02.124-132

Abstract

Bitter seed (Andrographis paniculata (Burm. f.) Wall. ex Nees) is a medicinal species used in phyto pharmacy and known as ‘the king of bitterness’. Bitter seeds contain andrographolide, a diterpenoid lactone which is widely used in Indian medicine for snake bites and for the treatment of hepatitis. The research was conducted at the Seed Management Unit experimental field of the Indonesian Spice and Medicinal Plants Research Institute (IMACRI) and at the Seed Quality Testing Laboratory of IPB University, Indonesia, from January until May 2021. The objectives of the study is to determine the best germination test and effective breaking dormancy techniques to increase the germination percentage of bitter seeds. The study was arranged in a completely randomized design with two-factors. The first factor was seed treatments, i.e., control/untreated, chemical scarification with 5% H2SO4 for 10 minutes, and 5% H2SO4 followed by a 100 ppm GA3 soaking treatment. The second factor conducted was germination test methods according to International Seed Testing Association, i.e., germinating the seeds on the top of paper and in pleated paper. Our study demonstrated that seed treatment with H2SO4 can break seed dormancy and speed up seed germination. Bitter seed germination on the pleated paper was higher than with the top-of-paper method. Bitter seed germination with the top-of-paper method was at 65 DAS and the final germination was at 80 days after sowing (DAS), and the final germination was 55.2%. With the pleated paper method, the seeds germinated at 30 DAS and the final germination was at 70 DAS, and the final the germination of 59.2%.
Quality and Production Potentials of Various Types of Taro Cormel Fauzan, Rafi; Widajati, Eny; Palupi, Endah Retno; Santosa, Edi
Journal of Tropical Crop Science Vol. 11 No. 02 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.02.137-146

Abstract

Taro is a promising food crop that can be developed as a significant source of carbohydrates; however, a major obstacle is the unavailability of high-quality planting materials. Due to the difficulty in obtaining seeds, taro cultivation generally relies on vegetative propagation materials such as cormels, cormlets, and stolons. The advantage of using vegetative propagation material is that it retains the same characteristics as the parent plant. This research aimed to evaluate the quality of various types of cormels as taro planting material, study the potential of different types of cormels on the growth and production of taro plants, and investigate the growth, development, and time required to produce cormels from various types of cormels and their potential in cormel seed production. The research consisted of two experiments: the first experiment evaluated the quality of several types of cormels from two taro accessions (S24 and S28) using a completely randomized design with two factors (accession and types of cormels—primary, secondary, and tertiary), while the second experiment studied the growth and development of cormels from various types of cormels. The best quality seeds were obtained from primary cormels directly attached to the main tubers, with the germination rate highest for primary cormels at 53.25%, followed by secondary cormels at 51.59%, and tertiary cormels at 39.42%. Accession S24 showed a faster emergence rate (30.26 days) compared to S28 (58.08 days), and in S24, there was no significant difference between types of cormels for this parameter. In contrast, in S28, primary cormels had a significant emergence advantage (33.65 days) over secondary and tertiary cormels (62.57 and 78.02 days, respectively). Additionally, primary cormels were formed 8 weeks after planting, while secondary and tertiary cormels appeared 12 weeks after planting. These findings suggest that primary cormels should be prioritized for use in taro propagation to improve growth, development, and yield, highlighting their potential in cormel seed production and offering a viable solution to the challenge of seed availability in taro cultivation.
Fruit and Seeds Development and Seed Germination of Andaliman Pepper (Zanthoxylum acanthopodium DC.) Fahmi, Mochammad; Widajati, Eny; Palupi, Endah Retno; Rostiana, Otih
Journal of Tropical Crop Science Vol. 13 No. 01 (2026): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.13.01.75-93

Abstract

Andaliman is a species found in the Lake Toba region of North Sumatra, Indonesia. To determine its optimal harvest time and the length of its physiological maturity, it is necessary to observe fruit development in new locations outside its natural habitat. Andaliman seeds are known for their low germination rates and are classified as endospermic seeds. This study aims to analyze the stages of fruit and seed development, evaluate the effects of seed treatments, and identify the most effective germination test methods for andaliman seeds. Seeds were treated with a 50% H2SO4 solution for 15 min and with a 200 ppm GA3 + 100 ppm kinetin solution for 48 hr. The seeds were then tested using pleated paper and top paper media to assess germination response. Changes in fruit coloration indicated various stages of development: intense yellowish-green at 17 weeks after anthesis, deep yellowish-green at 20 weeks, deep red at 23 weeks, and dark greyish-yellowish-brown at 26 weeks. During development, some fruits experienced embryo abortion, resulting in many empty or wrinkled seeds. The seeds reached physiological maturity at 23 weeks after anthesis, as indicated by a deep red fruit color, increased seed dry weight, and enhanced germination and seed growth rates. The treatment with GA3 and kinetin yielded the highest germination rate of 20%, with the pleated paper method proving to be the most effective for germination testing.
Co-Authors , Dina , Ramlafatma ,, Saipulloh . Giyanto Abdjad Asih Nawangsih Abdul Qadir Achmad Achmad Anak Agung Keswari Krisnandika Anggraini, Dwi Vista Annisa Nursoleha Ansella, Yohanda Anugrah, Mertya Arif, Mohamad Awang Maharijaya Bambang Sapta Purwoko Baran Wirawan Blair Moses Kamanga Cici Tresniawati Dahlan, Ade Muhamad Daniel Happy Putra Danu Kuncoro Devi Liana DEVI RUSMIN DEVI RUSMIN Devi Rusmin Didy Sopandie Dirgahani Putri Dwi Gery Febriyan Dwi Guntoro Dyah - Manohara DYAH MANOHARA Dyah Manohara Edi Santosa Edi Santosa Edi Santosa Edy Suprianto Efendi, Darda Endah Retno Palupi Endah Retno Palupi ENDANG MURNIATI Esti Rahayu Esty Puri UTAMI Esty Putri Utami Esty Putri Utami Fahmi, Mochammad Faiza C. Suwarno Faiza C. Suwarno Faiza Chairani Suwarno Faqih Udin Febriani, Lilis Yati Febriyan, Dwi Gery Gani Jawak Giyanto Giyanto Giyanto Giyanto Giyanto, dan Gundala, Belangie Tuahte Hadi, Putra K Haura Fikriyah Hakimah Herliyana Indahwardani Imroatus Sa’adah Indahwardani, Herliyana Irfan Firmansyah, Irfan Iswari Saraswati Dewi Juliana Maia Kamanga, Blair Moses Kikin H Mutaqin Kresnamurti Kurniasih Krisnandika, A. A. Keswari Kusumastuti, Hamiddah Intan Kusumastuti, Shinta Nugraheni Lilis Yati Febriani M.R. SUHARTANTO M.R. SUHARTANTO, M.R. Maisaroh Tanjung MANOHARA, DYAH Manohara, Dyah - Maryati Sari Masitadewi, Ervin Mathius, dan Nurita Toruan Meity Suradji Sinaga Memen Surahman Mia Kosmiatin Mohamad Arif Mohamad Arif Mohamad Rahmad Suhartanto Mubarak, Farahdina Muhamad Rezki Mauladi Muhamad Syukur Muhammad Afif Muhammad Machmud Nadiya Iftiwata Rahmah Nelly Fridayanti Ni Made Armini Wiendi Nizaruddin . Norul Dewi Susanti Nur Syam Ismaniza A., Siti Nur Syam Ismaniza A. Nurita TORUAN-MATHIUS Nutrita Toruan Mathius Okti Syah Isyani Permatasari Otih Rostiana Peni Handayani Permatasari, Okti Syah Isyani Pitri Ratna Asih Pradana, Adhitya Vishnu Pramudita, Listya Purwoko, Bambang Sapta Purwoko Putra K Hadi Putri Mian Hairani Putro, Trisno Yuwono Qadir , Abdul Qadir, Abdul Qudus Sabha Adhinugraha R Pelealu, Rian Virvian Hidayat R. Palupi, Endah Rafi Fauzan Raga, Yuan Rahmah, Nadiya Iftiwa Riski Meliya Ningsih RUSMIN, DEVI RUSMIN, DEVI Sabar Pramono Sani, Mutiyara Sarah Desmia Muchtar Sarah Desmia Muchtar Satrias Ilyas Satriyas Ilyas SATRIYAS ILYAS Selly Salma Selly Salma Shinta Nugraheni Kusumastuti Siti Fadhilah, Siti Sobir Sobir Sri Suharyati Vityaningsih Sri Wilarso Budi SUHARTANTO, M.R. Suhartanto, Muhammad Rahmad Sukartini Supijatno Surya Kurnia Putra, Dicky Suwarno, Faiza C. Tanjung, Maisaroh Tantri Palupi Tati Budiarti Tefa, Anna Tefa, Anna Tri Astuti Trikoesoemaningtyas Trisno Yuwono Putro Wawan Hermawan Willy Bayuardi Suwarno Yuyuk Agung Lastiandika Yuyuk Agung Lastiandika