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A Prenylated Flavonoid from the Methanol Fraction of Breadfruit Root (Artocarpus communis) Kristina Damiyanti; Ari Widiyantoro; Rudiyansyah Rudiyansyah
Jurnal Akademika Kimia Vol. 9 No. 4 (2020)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.436 KB) | DOI: 10.22487/j24775185.2020.v9.i4.pp237-244

Abstract

Breadfruit (Artocarpus communis) is a tropical fruit plant that belongs to the genus Artocarpus of the Moraceae family. Prenylated flavonoid compounds were isolated from the methanol fraction of breadfruit root wood using maceration, partitioning, and chromatography methods, while characterization was carried out through phytochemical tests and 1H-NMR spectrum data analysis. The K1D2 (P1) compound obtained was a yellow solid with a mass of 5 mg. The phytochemical test of the K1D2 (P1) compound gave positive results for the flavonoid group. The 1H-NMR spectrum data (CDCl3, 500 MHz) of compound K1D2 (P1) showed a typical chemical shift at δH 13.42 (1H, s, 5-OH) for the hydroxy groups associated with carbonyl; 7.68 (1H, d, J = 8.5 Hz, H-6 ′); 6.71 (1H, dd, J = 16.0; 7.1 Hz, H-10); 6.56 (1H, d, J = 16.0 Hz, H-9); 6.54 (1H, dd, J = 8.5; 1.8 Hz, H-5 ′); 6.46 (1H, s, H-3); 6.42 (1H, d, J = 1.8 Hz, H-3 ′); 6.26 (1H, d, J = 9.3 Hz, H-14); 5.42 (1H, d, J = 9.3 Hz, H-15); 3.94 (3H, s, 2′-OCH3); 2.47 (1H, m, H-11); 1.97 (3H, s, H-17); 1.69 (3H, s, H-18), and 1.10 (6H, d, J = 6.7 Hz, H-12 and H-13). Based on the results of phytochemical tests and analysis of 1H-NMR spectrum data compared with literature data, it showed that the isolated K1D2 (P1) compound is 4 ′, 5-dihydroxy-6,7- (2,2-dimethylpirano) -2'-methoxy- 8γ, γ-dimethylallylflavone (1).
Efektivitas ekstrak metanol akar pandan wangi (Pandanus amaryllifolius Roxb.) Sebagai antimalaria terhadap jumlah limfosit dalam darah mencit (Mus musculus) yang diinfeksi Plasmodium berghei Danang Mustofa; Muhammad Ibnu Kahtan; Diana Natalia; Mistika Zakiah; Ari Widiyantoro
Intisari Sains Medis Vol. 10 No. 2 (2019): (Available online: 1 August 2019)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (698.562 KB) | DOI: 10.15562/ism.v10i2.366

Abstract

Malaria is a infectious disease cause by protozoa with genus Plasmodium that is transmitted by female Anopheles mosquito. Our immune system which is role by lymphocyte works specifically when body infected by Plasmodium. The secondary metabolites of Pandan Wangi roots (Pandanus amaryllifolius Roxb.)  possess as antimalaria. The purpose of this research to know the activity of methanol exctract from roots of Pandan Wangi (Pandanus amaryllifolius Roxb.) as antimalaria on white male mice (Mus muscullus) lymphocyte count infected by Plasmodium berghei. This is a true experimental in vivo with completely randomized design. Antimalaria effect test used methanol extract from roots of Pandan Wangi with concentration 6.5%, 13% and 26%. Positive control group was given 3.74 mg/mL DHP (Dihydroartremisinin-Piperaquin), normal control group was not given any intervention, negative control group was given aquadest. Results of this research show extract with concentration 6.5%, 13% and 26% have the antimalaria effect show by the decrease of parasitemia percentage. In addition, concentration of 26% extract can increase the number of lymphocytes significantly. Extract of Pandan Wangi Roots have antimalaria effect and can increase the number of lymphocytes with the best concentration of 26%. The decreasing number of parasitemia level is not directly related to the increasing of lymphocyte
Antifeedaant Compound From Daruju Leaves (Acanthus Ilicifolius Linn) Against Eggplant Leaves Beetle (Epilachna Sparsa) Ade Mariadi; Ari Widiyantoro; Endah Sayekti
JURNAL KIMIA MULAWARMAN Vol 20 No 1 (2022)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v20i1.1127

Abstract

Daruju (Acanthus ilicifolius Linn) contain flavonoids, terpenoids, steroids, saponins, alkaloids and tannins that can be used as antifeedant compounds. The purpose of this study is to identify the active compound antifeedant from daruju leaves against the eggplant leaf beetle (Epilachna sparsa). The antifeedant activity test was carried out on the methanol extract, the partitioned fraction and the isolate. This study was began by maceration of daruju leaf powder (1000 g) with methanol that obtained methanol extract of 130.3 g. The methanol extracts were then separated by partitioning that obtained n-hexane, dichloromethane, ethyl acetate and methanol fraction. Antifeedant activity test against eggplant leaf beetle (Epilachna sparsa) showed the dichlormethane fraction at a concentration of 6 ppm had the best activity compared to other fractions with an activity of 90.62%. Separation and purification of the dichloromethane fraction to obtain isolation was carried out by column chromatography. The isolate showed 96.1% antifeedant activity at a concentration of 6 ppm. Based on 1H-NMR spectrum showed isolate is p-Oxy-2-ethylhexyl benzaldehyde.
KARAKTERISASI METABOLIT SEKUNDER DARI DAUN MANGROVE Rhizopora stylosa SEBAGAI ANTIOKSIDAN (CHARACTERIZATION OF SECONDARY METABOLITE MANGROVE LEAVES Rhizopora stylosa SPECIES AS ANTIOXIDANTS) Septian Triadi Syahputra; Ajuk Sapar; Ari Widiyantoro
Indonesian Journal of Pure and Applied Chemistry Vol 5, No 1 (2022)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.323 KB) | DOI: 10.26418/indonesian.v5i1.53566

Abstract

Mangrove leaves or also known as “bakau” by the people of Mempawah Regency, which has (Rhizopora stylose) species containing alkaloids, flavonoids, steroids, terpenoids, phenolics, and saponins that can be used as antioxidant compounds. This study was aimed to determine the antioxidant activity of extracts and secondary metabolite fractions, and also to determine the characteristics of isolate compounds from mangrove leaves (Rhizopora stylose). Maceration of 1.57 kg of dry powder of mangrove leaves (Rhizopora stylose) with methanol as a solvent obtained a crude extract of 141.13 g of methanol with a percent yield of 8.98%. Partitioning the crude methanol extract obtained the n-hexane, dichloromethane, ethylacetate, and residue fractions by weight and percent yield, respectively, namely 6.95 g (0.44%), 26.74 g (1.70%), 26 .66 (1.69%), and 25.75 g (1.64%). The results of the antioxidant activity test showed that the dichloromethane fraction had a better antioxidant activity with an IC50 value of 121.314 g/mL. Separation and purification of the dichloromethane fraction to obtain isolates were carried out by column chromatography in order to obtain pure isolates which were characterized by the appearance of a single spot on the tested plate with various eluents, namely, isolate F7. The 1H-NMR data for isolate F7 showed chemical shifts for aromatic protons of 8.52 ppm, 7.70 ppm, 7.67 ppm, protons for tertiary C of 5.41 ppm, and protons for methyl and methylene of 3.50 ppm, 3.47 ppm, 1.59 ppm, 1.23 ppm, 1.05 ppm, and 0.84 ppm. H-NMR data showed that isolate F7 had similarities with the chemical shift of p-Oxy-2-Ethylhexyl benzaldehyde, namely 7.71 ppm, 7.54 ppm, 4.22 ppm, 1.68 ppm, 1.35 ppm, 1.32 ppm, 1.30 ppm, 0.90 ppm but different in the alkoxy substituent position from isolate F7 which was in the meta position
ENERGI CELAH-PITA MATERIAL TiO2/KOMPLEKS LOGAM-KLOROFIL (M=Zn2+, Co2+) DARI DAUN SINGKONG (Manihot esculenta crant) (BANDGAP ENERGY OF MATERIAL OF TiO2/METAL-CHLOROPHYLL COMPLEX (M= Zn2+, Co2+) FROM CASSAVA LEAVES (Manihot esculenta crant)) Arif Sulaiman; Imelda Hotmarisi Silalahi; Anis Shofiyani; Ari Widiyantoro; Harlia Harlia
Indonesian Journal of Pure and Applied Chemistry Vol 5, No 1 (2022)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.963 KB) | DOI: 10.26418/indonesian.v5i1.49364

Abstract

Synthesis and characterization of TiO2 films sensitized with chlorophyll and metal-chlorophyll complex (M=Zn2+, Co2+) were carried out. Chlorophyll isolated from cassava leaves shows absorption peaks on the UV-Vis spectra in the Soret band area (415 nm) and Q band area (665 nm). The metal-chlorophyll complex was prepared from the reaction of the isolated chlorophyll and ZnCl2 (for Zn2+-chlorophyll); CoCl2.6H2O (for Co2+-chlorophyll) under reflux condition at 65 ºC with a mole ratio of 1:1. The UV-Vis spectra of the isolated products showed a hypsochromic shift to 410 nm and 660 nm for Zn2+-chlorophyll whereas the spectrum of the Co2+-chlorophyll product demonstrated the shifts to 403 nm and 661 nm. These hypsochromic shifts are proposed to be a metal-to-ligand charge transfer (MLCT) transition as a result of chlorophyll metalation. The FTIR spectra of chlorophyll and metal-chlorophyll complexes have a similar pattern despite changes in the absorption of vibrational energy in several functional groups. The absorption of the C=N group shifted from 1372 cm-1 to 1368 cm-1 for both of the complexes, the C=C group of aromatic shifted from 1451 cm-1 to 1447 cm-1 (Zn2+-chlorophyll) and from 1451 cm-1 to 1445 cm-1 (Co2+-chlorophyll), and the C=O group of ketones shifted from 1627 cm-1 to 1645 cm-1 (Zn2+-chlorophyll) and from 1627 cm-1 to 1646 cm-1 (Co2+-chlorophyll). The shift pattern of the absorption peaks on the FTIR spectra indicates the coordination of metal ions towards the N atom in the pyrrole ring in the porphyrin structure of the chlorophyll. When the isolated chlorophyll as well as the chlorphyll complexes was sensitized on the surface of TiO2, variation of bandgap energy was observed. The calculation using the Tauc Plot method resulted in the bandgap energy of films of TiO2 at 3.20 eV, TiO2/chlorophyll at 2.97 eV, TiO2/Zn2+-chlorophyll at 2.87 eV, and TiO2/Co2+-chlorophyll at 2.90 eV
Evaluation of the Antiplasmodial Properties of Andrographis paniculata (Burm.f.) and Peperomia pellucida (L.) Kunth Nurhayati Bialangi; Mohamad Adam Mustapa; Yuszda Salimi; Weny Musa; Ari Widiyantoro; Agus Malik Ibrahim; Boima Situmeang; Julinton Sianturi
Indonesian Journal of Chemistry Vol 23, No 1 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.74481

Abstract

Plasmodium species are the infectious agents that are responsible for malaria, a disease that claims the lives of approximately 400,000 people annually. The fact that drug resistance against malaria is on the rise suggests that new antimalarial compounds need to be discovered. It is well known that medicinal plants present the best opportunity for the identification of novel antimalaria chemicals. Both the Andrographis paniculata (Burm.f.) and Peperomia pellucida (L. Kunth) species have been tested for their antiplasmodial ability against the Plasmodium falciparum strain. The P. pellucida (L. Kunth) species has also been subjected to in vitro and in vivo biological research. P. pellucida was used to isolate the steroid known as 3-hydroxy-24-ethyl-5,22-cholestadiene (1) and the triterpenoid known as 3-hydroxy-9-lanosta-7,24E-dien-26-oic acid (2). Both compounds were then tested for their activity in vitro. In the mice model, triterpenoid 2 had a substantial chemo-suppressive impact.
PEMBERDAYAAN KELOMPOK TANI JAHE DESA RASAU JAYA UMUM MELALUI PELATIHAN DAN PENDAMPINGAN TANAMAN OBAT TRADISIONAL Ari Widiyantoro; Muhammad Akib Yuswar; Uray Ristian; Nafsiatun
Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Vol 3 (2022): PROSIDING SEMINAR NASIONAL PENGABDIAN KEPADA MASYARAKAT - SNPPM2022
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abstract The Ginger Farmer Group in Rasau Jaya Umum Village is a ginger farmer group that only produces ginger rhizomes which are directly sold to collectors. This results in the farmers being less empowered to improve their welfare. Therefore, training and assistance is needed to improve the knowledge and skills of the Ginger Farmer Group in Rasau Jaya Umum Village in understanding various medicinal plants and their processing so as to increase the selling value of agricultural products. The implementation of activities is carried out in the form of training and assistance regarding medicinal plants and their processing. Based on observations made during the activity and then giving a questionnaire, data obtained that members of the ginger farmer group in Rasau Jaya Umum Village were enthusiastic about participating in the activity by 100%, increasing their knowledge by 30%, skills in processing medicinal plants increased by 40%. This condition is a trigger for improving the welfare of the ginger farmer group in Rasau Jaya Umum Village. Abstrak Kelompok Tani Jahe Desa Rasau Jaya Umum merupakan kelompok tani jahe yang hanya menghasilkan rimpang jahe yang langsung dijual ke pengepul. Hal ini mengakibatkan para petani kurang berdaya untuk meningkatkan kesejahteraannya. Oleh karena itu diperlukan pelatihan dan pendampingan untuk meningkatkan pengetahuan dan keterampilan Kelompok Tani Jahe Desa Rasau Jaya Umum dalam memahami berbagai tanaman obat dan pengolahannya sehingga dapat meningkatkan nilai jual hasil pertanian. Pelaksanaan kegiatan dilakukan dalam bentuk pelatihan dan pendampingan mengenai tanaman obat dan pengolahannya. Berdasarkan observasi yang dilakukan selama kegiatan kemudian dilakukan pemberian kuisioner diperoleh data bahwa anggota Kelompok Tani Jahe Desa Rasau Jaya Umum antusias mengikuti kegiatan sebesar 100%, meningkat pengetahuannya sebesar 30%, dan keterampilan pengolahan tanaman obat meningkat 40%. Kondisi ini menjadi pemicu peningkatan kesejahteraan Kelompok Tani Jahe Desa Rasau Jaya Umum.
Senyawa Antiinflamasi dari Fraksi Etil Asetat Akar Pandan Wangi (Pandanus amaryllifolius Roxb.) Terhadap Udema Kaki Mencit Terinduksi Karagenan Diva Feraldy; Endah Sayekti; Ari Widiyantoro
Jurnal Fitofarmaka Indonesia Vol 10, No 1 (2023): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jffi.v10i1.885

Abstract

Inflammation can occur due to insect bites, toxins, burns and blows. Inflammation can be cured using Non-Steroid Anti-Inflammatory Drugs (OIANS). Fragrant pandan root is thought to have anti-inflammatory activity because it contains alkaloids and flavonoid compounds. This study aims to determine the activity and characteristics of anti-inflammatory compounds from extracts, fractions and isolates of ethyl acetate root of fragrant pandan (Pandanus amaryllifolius Roxb.) against carrageenan-induced foot edema of mice. The process of isolation of fragrant pandan roots includes stages such as maceration, partitioning and chromatography. The results of the study The methanol extract had good activity at a dose of 200 mg/kg BB for a period of time (t3) 51.7%; the methanol fraction had good activity at a dose of 200 mg/kg BB in the time span (t3) 38.2%; the ethyl acetate fraction had good activity at a dose of 200 mg/kg BB over a period of time (t3) 56.9%; the dichloromethane fraction had good activity at a dose of 200 mg/kg BB in the time range (t3) 59.2%; the n-Hexane fraction had good activity at a dose of 200 mg/kg BB for a period of time (t3) 37.1%; and isolates had good activity at a dose of 50 mg/kg BB over a period of time (t3) 35.6%. K2 isolate from the ethyl acetate fraction was characterized using 1H-NMR with DMSO solvent at a frequency of 500 MHz and 13C-NMR with DMSO solvent at a frequency of 125 MHz. K2 isolate from the ethyl acetate fraction had similarities to Pandalisines A.
SENYAWA SITOTOKSIK DARI FRAKSI DIKLOROMETANA DAUN PANDAN WANGI (Pandanus amaryllifolius Roxb.) TERHADAP SEL KANKER PAYUDARA MCF-7 Ari Widiyantoro
Jambura Journal of Chemistry Vol 5, No 1 (2023): February
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34312/jambchem.v5i1.16539

Abstract

Pandan Wangi (Pandanus amaryllifolius Roxb.) is the plant that contains secondary metabolites with high antioxidant activity. These various activities include antibacterial, antidiabetic and hepatoprotective. This research was conducted to determine the cytotoxic activity and to determine the characteristics of isolates from dichloromethane fraction. This research was conducted in several stages including maceration, partitioning, and chromatography. The results showed that the isolate from dichloromethane fraction of pandan leaves had moderate cytotoxic activity against MCF-7 breast cancer cells with an IC50 of 155.0551 g/mL. The isolate from dichloromethane fraction was characterized 1H-NMR analysis (DMSO, 500 MHz), predicted as alkaloid group with a pyrrolidin skeleton. The chemical shift showed H 8.08 ppm (1H, s), 7.68 ppm (6H, m), 7.31 ppm (10H, m), 7.10 ppm (5H, s), 4.22 ppm (7H, m), 3.54 ppm (13H, s ), 3.36 ppm (11H, s), 3.16 ppm (2H, s), 2.64 ppm (2H, s), 2.36 ppm (2H, s), 1.98 ppm (1H, s), 1.65 ppm (12H, m), 1.45 ppm (12H, m), 1.29 ppm (19H, m), 1.17 ppm (5H, t), 1.10 ppm (4H, m), 1.06 ppm (3H, d), 0.99 ppm (6H, m), 0.79 ppm (24H, m) dan 0.51 ppm (17H, m).
METABOLIT SEKUNDER DARI FRAKSI AKTIF AKAR PANDAN (Pandanus amaryllifolius roxb.) YANG BERSIFAT ANTIFEEDANT TERHADAP Epilachna sparsa (SECONDARY METABOLITES OF THE ACTIVE FRACTION OF PANDAN ROOT (Pandanus amaryllifolius roxb.) AS ANTIFEEDANT AGAINST Epilachna sparsa) Ari Widiyantoro; Harlia Harlia; Annisa Dyah Astari Putri
Indonesian Journal of Pure and Applied Chemistry Vol 6, No 3 (2023)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/indonesian.v6i3.71213

Abstract

Pandan wangi (Pandanus amaryllifolius Roxb.) contains flavonoids, terpenoids, steroids, saponins, alkaloids and tannins which can be used as antifeedant compounds. This study aims to determine the antifeedant activity and to identify of secondary metabolites from pandan roots extract against Epilachna sparsa. The antifeedant activity test was carried out on the methanol extract and the partition yeild fraction. The research was carried out by maceration of pandan root which had been mashed into powder with methanol solution to obtain a methanol extract of 86.51 g. The extract was then separated by partitioning to obtain methanol fraction, ethyl acetate fraction, dichloromethane fraction and n-hexane fraction. The methanol extract and fraction from partition results were then tested for antifeedant activity against Epilachna sparsa. The results showed that at a concentration of 6% the methanol extract had an antifeedant activity of 59.37 ± 2.11 % against Epilachna sparsa. The fractions resulting from the partition included methanol, ethyl acetate, dichloromethane and n-hexane fractions at a concentration of 6% respectively showing antifeedant activity of 81.25 ± 3.56 %; 75.00 ± 2.99 %; 37.50 ± 3.77 % and 21.18 ±1.23 %. Based on the antifeedant activity test results, the methanol fraction showed the best activity. Furthermore, the methanol fraction was separated and purified by chromatographic techniques. The isolate obtained was 53.11 mg in the form of white needle crystals and had a melting point of 135-138oC. The isolate based on the phytochemical test showed positive for flavonoid
Co-Authors Ade Mariadi Agus Malik Ibrahim Ahmad Yani Nelly Wahyuni Lia Destiarti Ajuk Sapar Andi Hairil Alimuddin Anis Shofiyani Anis Shofiyani Annisa Dyah Astari Putri Annisa Firdaus, Navisa Arif Sulaiman Aritonang, Anthoni B. Banu Prasetya Boima Ramses Situmeang Danang Mustofa Diana Natalia Diana Natalia Dila, Gata Diva Feraldy Elvi Rusmiyanto Pancaning Wardoyo, Suci Lestari, Mukarlina, Endah Sayekti Gusrizal Gusrizal Haris, Muhammad Faisal Harlia Harlia Harlia Harlia Harlia Harlia Harlia Harlia Harlia Harlia Herlinda Djohan Ihsan, Bagus Muhammad Ikhwan, Darul Imelda Hotmarisi Silalahi Indrawati, Ratih Indri Kusharyanti Jayuska, Afghani Julinton Sianturi Kahtan, Muhammad Ibnu Kamarullah Kristina Damiyanti Kustiati Kustiati M. Irfani Hendri Mardhia, Mardhia Maryati Maryati Maulidiyah Salim Millenia, Millenia Mistika Zakiah Mohamad Adam Mustapa Muhammad Akib Yuswar Muhammad Akib Yuswar Muhammad Faisal Haris Muhammad Ibnu Kahtan Muhammad Ibnu Kahtan Nafsiatun Nelly Wahyuni Nurhayati Bialangi Nurlina, Nurlina Nurul Asikin Puji Ardiningsih Putri, Annisa Dyah Astari Putri, Rinka Carolina Rachmadi, Octavian Rahayu, Warsi Kurnia Ratna Wardani Ratnawaty, Gervacia Jenny Ratu Safitri Risa Nofiani Rr. Erni Kusuma Putri Rudiyansyah Rudiyansyah Rudiyansyah Rudiyansyah Sadenta, Deby Pascalia Sari Damayanti Sari, Emilda Septian Triadi Syahputra Sharon Angelia Shofiyani, Anis Siti Khotimah Sulistyo, Luthfi Imam Susanto, Dhessy Thamrin Usman Thamrin Usman Titin Anita Titin Anita Zahara, Titin Anita Unang Supratman Uray Ristian Wahyuningsih, Aprilia Tri Warsidah, Warsidah Weny Musa Wibowo, M. Agus Wibowo, Muhamad Agus Winda Rahmalia Yuswar, Muhammad Akib Yuszda K Salimi Zaharah, Titin Anita