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In vitro growth of Coelogyne hybrid (Coelogyne pandurata x Coelogyne rumphii) on medium 2.4D and BAP Ahmad Yunus; Sri Hartati; Samanhudi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 3 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i3.66556

Abstract

Coelogyne is a genus of ornamental orchids renowned for its distinctive floral characteristics and high conservation and economic value, particularly Coelogyne pandurata, which is commonly referred to as a black orchid. Efforts in cultivating black orchids in vitro require media modification with the addition of growth regulators to optimize plant development. Growth regulators that can be used include auxins and cytokinins. This study evaluates the effects of 2.4D (2,4-Dichlorophenoxyacetic Acid ) and BAP on the in vitro growth and shoot proliferation of Coelogyne pandurata X Coelogyne rumphii hybrid seedlings. The experiment used a completely randomized design with two factors: 2,4-D and BAP, each with four concentration levels (0, 0.5, 1, and 1.5 ppm), resulting in 16 treatment combinations. The variables observed were the percentage of callus formation, callus color, shoot number, root number, leaf number, and plant length. The results showed that the optimal callus growth was achieved with a combination of 2,4-D at 0.5 ppm and BAP at 0.5 ppm, resulting in 100% callus formation. The 2,4-D treatment yields the optimum results in terms of leaf numbers (23.56) and plant height (2.15 cm), while the 1.5 ppm BAP treatment achieves the optimum results in terms of leaf numbers (31) and shoot numbers (22.39). Keywords: auxin; black orchid; callus; cytokinin; plant growth regulator
Diversifikasi Tanaman Pekarangan Dengan Tanaman Alpukat Untuk Meningkatkan Gizi Keluarga Hartati, Sri; Yunus, Ahmad; Nandariyah, Nandariyah; Yuniastuti, Endang; Pujiasmanto, Bambang; Purwanto, Edi; Samanhudi, Samanhudi; Sulandjari, Sulandjari; Ratriyanto, Adi; Prastowo, Sigit; Manurung, Ida Rumia; Suryanti, Venty; Susilowati, Ari; Artanti, Anif Nur; Mulyani, Sri; Dirgahayu, Paramasari
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 11, No 2 (2022): November
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v11i2.61199

Abstract

Alpukat (Persea americana) merupakan buah yang sudah sangat dikenal dan digemari oleh masyarakat. Alpukat diketahui memiliki khasiat sebagai antioksidan, antidiabetik dan efek hipolipidemik. Nutrisi yang terkandung dalam buah alpukat mampu membantu memenuhi kebutuhan nutrisi harian tubuh. Namun, cara budidaya tanaman Alpukat  sangat berpengaruh pada khasiat atau kualitas dan kuantitas dari produk yang dihasilkan. Sehingga diperlukan pemahaman tentang cara budidaya tanaman Alpukat kepada masyarakat. Tujuan yang ingin dicapai dalam kegiatan ini adalah agar masyarakat lebih memahami cara budidaya tanaman alpukat sebagai tanaman pekarangan dan mengetahui manfaat buah Alpukat dalam meningkatkan gizi keluarga. Kegiatan pengabdian sudah dilaksanakan dengan metode penyuluhan dengan penyampaian materi tentang budidaya tanaman Alpukat, manfaat Alpukat, serta berbagai olahan Alpukat sedangkan pada akhir sesi dilakukan pemberian bibit Alpukat serta pupuk pada masing-masing peserta. Kegiatan yang sudah terlaksana ini bermanfaat untuk menambah pengetahuan dan keterampilan masyarakat khususnya masyarakat Kelurahan Wonokarto tentang budidaya tanaman Alpukat dan keanekaragaman manfaat Alpukat untuk kesehatan.Kata Kunci: alpukat; budidaya; penyuluhan; pupuk
GROWTH RESPONSE AND DROUGHT TOLERANCE OF THREE ECHINACEA PURPUREA ACCESSIONS AT DIFFERENT WATERING INTERVALS Rina Puji Lestari; Ahmad Yunus; Samanhudi
Agrisaintifika: Jurnal Ilmu-Ilmu Pertanian Vol. 10 No. 2 (2026): Agrisaintifika
Publisher : Universitas Veteran Bangun Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32585/ags.v10i2.8144

Abstract

Drought is widely recognized as one of the most critical environmental constraints limiting plant growth and development, particularly in medicinal species such as Echinacea purpurea. This research was conducted to assess the growth performance and drought tolerance of three E. purpurea accessions exposed to different watering regimes. The study employed a completely randomized design arranged factorially, consisting of two experimental factors, namely accession (three accessions) and watering interval (1, 3, 5, and 7 days), with each treatment replicated five times. Statistical analysis was performed using analysis of variance (ANOVA), and significant differences among treatments were further evaluated using Duncan’s Multiple Range Test (DMRT) at the 5% probability level. The findings revealed that watering interval had a significant influence on plant growth, while the effect of accession was less pronounced for most observed variables. Daily watering resulted in superior growth performance, as reflected by greater plant height and a higher number of leaves. In contrast, extending the watering interval led to a marked reduction in growth. Among the evaluated accessions, accession 3 consistently exhibited better adaptation under limited water availability. Furthermore, differences in root-to-shoot ratio under drought conditions suggest the occurrence of adaptive mechanisms that enable plants to cope with water deficit stress. Overall, the results indicate that watering interval plays a more decisive role than accession in determining the growth of E. purpurea, whereas accession 3 possesses the greatest potential for drought tolerance. Keywords: drought stress, Echinacea purpurea, growth, drought tolerance
Penambahan Bahan Organik dan NAA terhadap Pertumbuhan Anggrek Hasil Persilangan Coelogyne secara In Vitro Sri Hartati; Ahmad Yunus; Dwi Alma Indah Puji Astuti
Agrosains : Jurnal Penelitian Agronomi Vol 26, No 2 (2024): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v26i2.79509

Abstract

Orchids are ornamental plants that have a high selling value so they are very prospective in the world of the ornamental plant business. This high economic value means that market demand for orchid commodities tends to increase. Efforts made to meet the need for orchids in the market are by propagating orchids using in vitro culture. This research aims to determine the effect of adding organic materials and NAA on the growth of orchids resulting from crossing Coelogyne pandurata and Coelogyne rumphii in vitro. This research used a completely randomized design (CRD) with two factors, namely the type of organic material (tomato, carrot and potato) and the concentration of ZPT NAA (0, 1 and 2 ppm). The research results showed that the addition of potato and tomato organic materials to the VW base medium was able to stimulate plant growth. The added NAA concentrations of 1 and 2 ppm are able to stimulate optimal plant growth. The combination of adding tomato organic matter and 1 ppm NAA concentration had the best growth in the number of roots and leaves, while adding tomato organic matter and 2 ppm NAA had the best growth in the number of shoots. The combination of adding potato organic material and 2 ppm NAA can stimulate plant height growth.
Produktivitas dan Kandungan Protein Beras Mutan M6 Hasil Iradiasi Sinar Gamma pada Benih Padi Varietas Mentik Susu Tiwi Rachmawati; Ahmad Yunus; Parjanto Parjanto
Agrotechnology Research Journal Vol 7, No 1 (2023): Agrotechnology Research Journal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrotechresj.v7i1.73844

Abstract

Mentik Susu is a popular rice germplasm in Indonesia. Due to field constraints in Mentik Susu rice cultivation, farmers switched to better varieties with higher yields. Farmers switch to superior types with high yields due to high plant posture, long harvest life, and low yields. Rice plants usually have a protein content of 4-5% per gram while without given radiation. It will be expected that after receiving gamma rays, the protein content in mutant plants will increase up to 7-8% per gram. The aim of this research was to evaluate the productivity and protein content of rice grain of 35 mutant strains of rices that could be developed into new varieties. The research took place at the Tegalgondo Rice Seed Garden Agricultural Land between June to October 2020. This research used Randomized Complete Block Design method with a single factor. Each treatment was repeated three times. The data obtained was analyzed with analysis of variance and followed with Duncan’s Multiple Range Test at a 5% level if any significant influences. The results of this research showed productivity of 35 Mentik Susu rice lines ranged from 4.28 to 6.69 ha-1, with either a high protein content ranging from 5-8% per gram and intermediate high yield potential strains G63T11-B6-T27(1), G15T5-14-B5-T60, G15T5-14-B5-T46, G11T22-19-B1-T48, G15T5-14-B3-T36, M-MS2-G15T3-2-5 -T27, M-MS2-G18T7-4-12-T13, M-MS2-G18T7-4-9-T12. These strains might be cultivated as potential candidates for new high-yielding varieties, which would then be evaluated for higher yields or multilocation tests before becoming published as new varieties.
Effect of Water Availability on the Growth and Secondary Metabolites of Zodia (Evodia suaveolens) Andriyana Setyawati; Edi Purwanto; Ahmad Yunus; Samanhudi Samanhudi; Amalia Tetrani Sakya; Muji Rahayu; Djoko Purnomo; Retna Bandriyati Arniputri; Gani Cahyo; Qonita Rahma Dwiyanti; Saat Egra; Okky Talitha; Alfida Muthi’ah
Agrosains : Jurnal Penelitian Agronomi Vol 25, No 2 (2023): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v25i2.81528

Abstract

Zodia are included in medicinal plants because it contains secondary metabolites such as flavonoids, tannins, alkaloids, steroids/triterpenoids that have many benefit and also as mosquito repellents. The growth and content of secondary metabolites in plants can be affected by environmental factors such as water availability. Research on the cultivation of this crop is still limited. Environmental modification in cultivation is needed to increase its secondary metabolites, and the study of the potential of this plant as medicine are necessary. This study aimed to determine the effect of water availability on the growth and secondary metabolites of zodia. The research was carried out in July-November 2021 in Greenhouse, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta. The method used was a completely randomized design, one treatment factor with 4 levels namely, 100, 80, 60 and 40% of field capacity. Each treatment was repeated 6 times so that there were 24 experimental polybags. The data analysis used is Analysis of Variance, and the Duncan’s Multiple Range Test at the 5% level. The results showed that the availability of water at a level of 60% field capacity gave the best average growth parameters (plant height and number of leaves) among other treatments. The flavonoid content in each treatment showed the same average. The results of the GCMS (Gas Cromatography and Mass Spectroscopy) test of zodia leaves showed that there were dominant bioactive compounds, namely ethylbenzene, octadecamethyl cyclononasiloxane, pentadecanoid acid and heptadecanoid acid. It is suspected that the content of these compounds has the potential as antifungal, antibacterial, antioxidant and anticancer.
Effect of Auxin and Cytokinin on Orchid Subculture Resulting from Coelogyne Crosses Sri Hartati; Ahmad Yunus; Iistikharoh Iistikharoh
Journal of Biodiversity and Biotechnology Vol 3, No 2 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i2.80626

Abstract

The black orchid or Coelogyne is one of the native orchids of Kalimantan, widely cultivated in Indonesia. Several species of Coelogyne orchids have been crossed to obtain new superior-quality plants. Modifying the media, such as giving PGR, is necessary for good plant multiplication results. This research was conducted by changing MS growing media with ZPT BA and NAA. This experiment aimed to determine the effect of ZPT BA and NAA on the growth of orchid subcultures from a cross between Coelogyne pandhurata and Coelogyne rumphii. The study used a completely randomized design (RAL) with 2 factors, namely ZPT BA and NAA, each having a concentration of 0 ppm, 0.2 ppm; 0.4 ppm, and 0.6 ppm with 16 treatment combinations and 4 replications. They were implemented at the Laboratory Plant Physiology and Biotechnology, Faculty of Agriculture, UNS Surakarta, from November 2020 to March 2021. The variables observed included the number of shoots, plant length, plant weight, number of leaves, number of roots, root length, when roots appeared, and when shoots appeared. The results showed that BA 0.6 ppm gives the best output on the growth of the number of shoots, and NAA concentration of 0,2 ppm gives the best results on the variable number of roots. The combination of BA 0.6 ppm and NAA 0.2 ppm gave the best results on plant weight growth.
Yield of the Mutant (M6) Short Stem of Mentik Wangi Rice Varieties Resulting from Gamma Ray Irradiation 300 Gray Ahmad Yunus; Rizky Mulyawati; Parjanto Parjanto
Journal of Biodiversity and Biotechnology Vol 1, No 2 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i2.56437

Abstract

Mentik Wangi is one of Indonesia's most popular rice germplasm sources due to its delicate, fluffy texture and arom aroma. Farmers switch to better varieties with high yields due to field challenges in cultivating fragrant rice. The plants are quite tall, harvest time is long, and yield is low; therefore farmers switch to superior varieties with high yields. The purpose of this research was to obtain information on how much yield of 14 mutant rice lines produce and to find mutant lines with short stems and high yields that could be developed to new varieties. The research was conducted at the Tegalgondo Rice Seed Garden Agricultural Land between June to October 2020. This study used a Completely Randomized Block Design (CRBD) with a single factor. Each treatment was repeated three times. The data obtained analyzed with analysis of variance and followed with Duncan Multiple Range Test at 5% level if any significant influences were obtained. The results of this research showed that 14 Mentik Wangi rice lines yields were ranging from 6.70 to  8.21 tons ha-1.Stems from 9 lines with high yields were M6-MW3-G10-14-2, M6-MW3- G12-1-17,  M6-MW3-G5-21-3,  M6-MW3-G5-21-14,  M6-MW3-G6-17-14-4,  M5-MW3-G6-20-9-16,  M6-MW3-G1-1-2  M5-MW3-G1-5-47,  M6-MW3-G6-10-9-26.  These lines were potential candidates for new high-yielding varieties, which would then considered to be evaluated for higher yields or multilocation tests before becoming an official new varieties.Keywords: mentik wangi; mutations; short stem; yield potential;
Genetic Diversity Analysis of Dendrobium sp Orchid from Indonesia based on Random Amplified Polymorphic DNA (RAPD) Ahmad Yunus; Sri Hartati; Fitria Riviqowati
Journal of Biodiversity and Biotechnology Vol 6, No 1 (2026)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v6i1.117657

Abstract

The orchid genus Dendrobium is a commodity from plants that are sold in pots, and Cut flowers are very popular in the world because they have flowers of various sizes, shapes, and colors. Orchid flowers of the Dendrobium genus flower throughout the year, so they are included in the promising cut flower industry. Information on genetic diversity and genetic relationships between orchids of the genus Dendrobium through molecular analysis techniques is needed in plant breeding programs and genetic resources. Orchids Dendrobium that have various phenotypes will increase the selling value of the commodity. The purpose of this study was to determine the genetic diversity of the orchid Dendrobium using RAPD markers. The scoring data were analyzed using NTSYS-pc (software Numeral Taxonomic System) version 2.02, which produced data in the form of cluster dendrograms. Genetic diversity of Dendrobium spp., analyzed with 5 primers RAPD, ranges from 43-76% and divides the dendrogram into 2 main clusters based on genetic similarity. The polymorphism of the 5 RAPD primers was very high, namely 91.6-100%.
Co-Authors Adi Ratriyanto Agastya Putra Pradipta Alfida Muthi’ah Alifia, Shafa Nur Aliya, Syafika Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya Andriyana Setyawati Andrizal Anif Nur Artanti, Anif Nur Anindita Dwi Yogi Sapta Ratri, Anindita Dwi Yogi Sapta Anindya Saras ARI SUSILOWATI ARI SUSILOWATI Arifiya Qifni Arviani, Syilla Asty Pratista Magdhalena Ayuhan, Ayuhan Bambang Puji Asmanto Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Bayu Aji Setyawan Damayanti, Farikha Dani Saputra, Hendra Dewanti, Felicitas Deru Djoko Purnomo Djoko Purnomo Djoko Purnomo Dwi Alma Indah Puji Astuti Dwi Harjoko Dwi Harjoko Edi Purwanto Edi Purwanto Edi Purwanto Edi Purwanto Eka Puji Lestari Eka Puji Lestari Endah Pratiwi, Sekar ENDANG YUNIASTUTI Estarini, Elisa Fajar Nugroho Febriyanti, Hani Fitniwilis, Fitniwilis Fitria Riviqowati Gani Cahyo Gani Cahyo Handoyo Glora Jayantie Hadiandra Harfiansyah Handayani, Ayu Mustika Hartono Hartono Heriyanto Heriyanto Herjuna Praba Wisesa Herlina Mega Puspitasari Himawan Joko Riswanda Himawan, Rifqi Hutapea, Zeriko Ida Ayu Putu Sri Widnyani Ida Rumia Manurung Iistikharoh Iistikharoh Ika Kurniaty Ika Lestari, Ayu Iwan Dewangga Joko Basuki Jundillah, Muhammad Labib Juniyansyah, Agung Kurnianto, Tri Lili Andriani Masa, Amin Padmo Azam Ma’arif, Samsul Meri Prasetyawati Moch. Galih Pranata, Moch. Galih Muhammad Amin, Hafiz Muhammad Rizqi, Farhan Muji Rahayu Mulyani, Sri Muslim, Izzul Nandariyah, Nandariyah Novika, Revi Gama Hatta Nurhayati Nurhayati Nurkholis Majid Nurwahyu, Ferryza Okky Talitha Ongko Cahyono Paramasari Dirgahayu Pardono Pardono Parjanto Parjanto Parjanto Parjanto Parjanto Prian Gagani Chamdareno, Prian Gagani Pricillia Galuh Pusparini Puji Harsono Puji Lestari Tarigan Putri Dewangga Maharani Putut Pamilih Widagdo, Putut Pamilih Qonita Rahma Dwiyanti Raden Dirgori Kuneng Brojokusumo Rahmanto Rahmanto Rahmawati, Sri Indah Rapiq, Rayhan Abdilah Ratri, Tito Mustikaningrum Retna Bandriyati Arniputri Retna Bandriyati Arniputri Retna Bandriyati Arniputri Rifdarmon Rina Puji Lestari Rini Mustikasari Kurnia Pratama Rizky Mulyawati Rosmaina Rosmaina Saat Egra Saberina Hasibuan Sagita, Andi Yolanda Samanhudi Samanhudi Saputra, Yance Saras, Anindya Satya Primasasta Zulhivan Setyadi, Hario Jati Sidabutar, Erni Veronica Sigit PRASTOWO Siswahyudianto Siti Jamilah, Lu’lu’ Siti Mardhika Sari siti wulandari Sobir Sobir Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Indah Rahmawati Sri Indah Rahmawati Sri Mulyani Sugiarti, Endang Sugiyarto Sugiyarto Sugiyarto Sugiyarto Sukaya Sukendah Sukendah Sukendah Sukendah Sulandjari Sulandjari Sulandjari Sulandjari, Sulandjari Supratikta, Hadi Syafriadiman, Syafriadiman Tiwi Rachmawati Udin Galang GA, Nur Ummah Karimah, Ummah Venty Suryanti Yulfarikaini, Yulfarikaini Yuli Widiyastuti Yuli Widyastuti Yuli Widyastuti Yulia Sari Zulfikri, Alif