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All Journal International Journal of Public Health Science (IJPHS) Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Jurnal Farmasi Klinik Indonesia Pharmacon Majalah Obat Tradisional Public Health of Indonesia Biomedical Journal of Indonesia Majalah Farmasi dan Farmakologi (Trends in Pharmacy and Pharmaceutical Sciences) Pharmasipha JFL : Jurnal Farmasi Lampung Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Lumbung Farmasi : Jurnal Ilmu Kefarmasian Jurnal Insan Farmasi Indonesia Medical Sains : Jurnal Ilmiah Kefarmasian Sciences of Pharmacy Jurnal Kesehatan Bakti Tunas Husada Borneo Journal of Pharmacy Medical Sains : Jurnal Ilmiah Kefarmasian Pharmacon: Jurnal Farmasi Indonesia
Lalu Muhammad Irham
Faculty Of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta
Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : Majalah Obat Tradisional

 1 
Cytotoxic Potential of Combination of Hibiscus sabdariffa L. Extract and Cisplatin against HeLa Cervical Cancer Cell Line: A Study of Antiproliferative Activity and Apoptosis Induction Nurani, Laela Hayu; Azzahra, Fara; Utami, Dwi; Edityaningrum, Citra Ariani; Guntarti, Any; Irham, Lalu Muhammad; Daud, Intan; Khairurrizki, Amanda; Putra, Ichsan Luqmana Indra; Ismiyati, Nur; Rofida, Siti; Sugiyanto, Sugiyanto
Majalah Obat Tradisional Vol 30, No 2 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.92440

Abstract

Cervical cancer is one of the most prevalent cancer types, making the development of effective anticancer agents critical. Cisplatin (CIS) is a drug that has been used to treat cancer, but it also affects normal cells. Hibiscus sabdariffa L. extract (HSE), which has the potential as an anticancer agent, can be developed as a co-chemotherapy with CIS. This study aimed to determine the potential of HSE as a co-chemotherapy with CIS against HeLa cervical cancer cells and determine specific and non-specific parameters of the studied extract. Hibiscus sabdariffa L. (HS) simplicia powder was macerated with 96% ethanol. An in vitro evaluation was carried out on antiproliferative activity by calculating the doubling time. Additionally, a selectivity test was conducted to calculate combination index (CI) values based on the microtetrazolium (MTT) method. Apoptosis mechanisms were explored based on immunocytochemical methods using p53, caspase, and Bax antibodies, followed by an observation of apoptotic induction using a flow cytometer. The HSE and CIS selectivity index values obtained for Vero cells and HeLa cells were 209 and 278, respectively. The antiproliferation test results showed that the combination of HSE and CIS could better extend the doubling time of cells compared to the negative control. This combination also demonstrated a strong synergistic effect, with a CI value of 0.001. The extract as a co-chemotherapy with CIS was capable of increasing the expression of p53, caspase-3, and Bax. The flow cytometry analysis results indicated that HSE could induce apoptosis. Based on the results on the IC50 and CI value of HSE, as well as on the doubling time and apoptosis induction of HSE-influenced HeLa cells, it is concluded that HSE has the potential as a co-chemotherapy against cervical cancer.
Co-Authors Abdi Wira Septama Abdi Wira Septama Abdi Wira Septama Abdi Wira Septama Abdi Wira Septama Abdi Wira Septama Adi Wira Septama Adnan Adnan Afief, Arief Rahman Ageng Brahmadhi Akrom, Akrom Ana Hidayati Anisa Devi Kharisma Wibowo Anisa Nova Puspitaningrum Anisa Nova Puspitaningrum Anisa Nova Puspitaningrum Anita Silas La’ah Any Guntarti Arief Rahman Afief Arief Rahman Afief Arief Rahman Afief Auren Nathania Ayu Lifia Nur Kartikasari Azzahra, Fara Barkah Djaka Purwanto Barkah Djaka Purwanto Bramadi Arya Cheung, Rocky Chita Widia Chong, Rockie Citra Ariani Edityaningrum, Citra Danang Prasetyaning Amukti Darmawi . Darmawi Darmawi Daud, Intan Delfi Iskardyani Denys Chichi Kusumastuti Didik Setiawan Donel S Dyah Aryani Perwitasari Eko Mugiyanto Faridah, Imaniar Febriana Astuti Firdayani Firdayani Firdayani Firdayani, Firdayani Firman Firman Fitri, Dwiki Fredrick Dermawan Purba Galuh, Tiwi Gina Noor Djalilah Gustinanda, Rizky Haafizah Dania Haafizah Dania Haafizah Dania Handayani, Eka Wuri Hanisari, Putri Henry Budiawan Prasetya Herjanti Ratnawiningsih Herlina, Tetie Ichsan Luqmana Indra Putra, Ichsan Luqmana Indra Ilham Alifiar Ilham alifiar Imaniar Noor Faridah Imaniar Noor Faridah Imaniar Noor Faridah Iwan Santoso, Iwan Kartikasari, Ayu Lifia Nur Khair, Riat Khairi, Sabiah Khairurrizki, Amanda La Malihi Laela Hayu Nurani La’ah, Anita Silas Leina Putri Zahra Lisza Niarisessa Made Ary Sarasmita Maliza, Rita MARIA BINTANG Maulida Mazaya Ma’ruf, Muhammad Medi Sushanti, Nining Melodia Rezadhini Melodia Rezadhini Menit Ardhiani Menit Ardhiani Muh. Deni Kurniawan Muhammad Fathurrahman Muhammad Yusron Maulana El-Yunusi Mutmainnah Siradjuddin Nanik Sulistyani Nathania, Auren Ni Made Ary Sarasmita Nining Medi Sushanti Nining Medi Sushanti Nining Sugihartini Novi Febrianti Nugraha, Media Fitri Isma Isma Nur Ismiyati Nur Rahayuningsih Pendicho Eko Yuliyanto Pradika, Jaka Pranata, Satria Pratama, Kharisma Puriyanto, Riky Dwi Purwanto, Barkah Djaka Puspitaningrum, Anisa Nova Rafiastiana Capritasari Rahmat Dani Satria Razali, Renardy Reza Renardy Reza Razali Rezadhini, Melodia Ria Indah Pratami Riat El Khair Riat Khair Rizka Novia Atmadani Rockie Chong Rocky Cheung Rocky Cheung Rocky Cheung Rudi, Nyoman S, Donel S, Yuni Salamah, Siti Hajar Sany Sapto Yuliani Saputri, Septiana Satria, Rahmat Dani Sianu, Rahman Simanjuntak, LB Siswandi Siswandi, Siswandi Siswanto, Lalu Muhammad Harmain Siti Rofida Siti Urbayatun Solikhah Solikhah, EfA Wakhidatus Sugiyanto - Suhandri, Wiwin Suhendra, Gugun Sulistiyani, Nanik Susan Fitria Candradewi Triantoro Safaria Triantoro Safaria Uswatun Chasanah Wahyu Rahmatulloh Wahyu Widyaningsih Wahyu Widyaningsih Wibowo, Anisa Devi Karisma Wibowo, Anisa Devi Kharisma Widyastuti, Lina Wigka Pawestri, Novianti Winarto Winarto Winarto Winarto Wirawan Adikusuma Wiwin Suhandri Wiwit Ade Fidiawati Wiwit Ade Fidiawati Woro Supadmi Woro Supadmi Wulandari, D Yudha Rizky Nuari Zahra, Leina Putri Zainul Amiruddin Zakaria Zakaria, Zainul Amiruddin Zukhruf Saputri, Ginanjar