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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Torani Journal of Fisheries and Marine Science Jurnal Natur Indonesia Widyariset BERITA BIOLOGI Jurnal Akuatika Indonesia Omni-Akuatika Acta Aquatica: Aquatic Sciences Journal Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Medik Veteriner Jurnal Penelitian Perikanan Indonesia Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Techno-Fish Journal of Aquaculture and Fish Health Jurnal Lemuru : Jurnal Ilmu Perikanan dan Kelautan Indonesia Nekton Jurnal Media Akuakultur Indonesia Jurnal Penelitian Belida Indonesia Journal of Fisheries & Marine

Odang Carman

Budidaya Perairan, Institut Pertanian Bogor, Indonesia

Author-ID : 245949

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Social Sciences Veterinary Other

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IDENTIFICATION OF MAJALAYA COMMON CARP STRAINS RESISTANT TO KHV INFECTION USING CYCA-DAB1*05 ALLELE AS THE MARKER Alimuddin Alimuddin; Mubinun Mubinun; Ayi Santika; Odang Carman; Irvan Faizal; Komar Sumantadinata
Indonesian Aquaculture Journal Vol 6, No 2 (2011): (December 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The Cyca-DAB1*05 allele of major histocompatibility complex class II genes is recently suggested to have a link with the European common carp strain resistant to koi herpesvirus (KHV). In this study, a set of specific primers for Cyca-DAB1*05 was designed and applied as a marker to identify broodstocks of majalaya common carp strain subsequently used as a candidate resistant to KHV infection. From a total of 23 broodstock subjected to PCR analysis, two female and male fish, both having (P) and no Cyca-DAB1*05 (N), were selected and then diallelly mated. Disease resistance of progenies from 10 crosses was determined by a survival analysis in pond rearing and a laboratory challenge-test using cohabitation method. The results have revealed that the average survivals of PxP progenies for pond rearing and KHV challenge test were 86% and 100% higher (P < 0.05) respectively compared to that of NxN fish. Survival rate of PxN/NxP progenies was significantly lower (P < 0.05) than that of PxP fish. Furthermore, PCR analysis showed that almost 91% progenies of PxP crosses seemed to have a KHV resistant gene marker. Thus, this study suggests that the marker is associated with the KHV resistance in majalaya common carp strain, and farming of PxP progenies can be useful to increase common carp production.

OVER-EXPRESSION OF GENE ENCODING FATTY ACID METABOLIC ENZYMES IN FISH Alimuddin Alimuddin; Goro Yoshizaki; Toshio Takeuchi; Odang Carman
Indonesian Aquaculture Journal Vol 3, No 2 (2008): (December 2008)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) have important nutritional benefits in humans. EPA and DHA are mainly derived from fish, but the decline in the stocks of major marine capture fishes could result in these fatty acids being consumed less. Farmed fish could serve as promising sources of EPA and DHA, but they need these fatty acids in their diets. Generation of fish strains that are capable of synthesizing enough amounts of EPA/DHA from the conversion of α-linolenic acid (LNA, 18:3n-3) rich oils can supply a new EPA/DHA source. This may be achieved by over-expression of genes encoding enzymes involved in HUFA biosynthesis. In aquaculture, the successful of this technique would open the possibility to reduce the enrichment of live food with fish oils for marine fish larvae, and to completely substitute fish oils with plant oils without reducing the quality of flesh in terms of EPA and DHA contents. Here, three genes, i.e. Δ6-desaturase-like (OmΔ6FAD), Δ5-desaturase-like (OmΔ5FAD) and elongase-like (MELO) encoding EPA/DHA metabolic enzymes derived from masu salmon (Oncorhynchus masou) were individually transferred into zebrafish (Danio rerio) as a model to increase its ability for synthesizing EPA and DHA. Fatty acid analysis showed that EPA content in whole body of the second transgenic fish generation over-expressing OmΔ6FAD gene was 1.4 fold and that of DHA was 2.1 fold higher (P<0.05) than those in non-transgenic fish. The EPA content in whole body of transgenic fish over-expressing OmΔ5FAD gene was 1.21-fold, and that of DHA was 1.24-fold higher (P<0.05) than those in nontransgenic fish. The same patterns were obtained in transgenic fish over-expressing MELO gene. EPA content was increased by 1.30-fold and DHA content by 1.33-fold higher (P<0.05) than those in non-transgenic fish. The results of studies demonstrated that fatty acid content of fish can be enhanced by over-expressing gene encoding enzymes involved in fatty acid biosynthesis, and perhaps this could be applied to tailor farmed fish as even better sources of valuable human food.

IMMUNE RELATED GENES EXPRESSION ANALYSIS IN KOI FISH AFTER VACCINATED WITH KOI HERPES VIRUS DNA VACCINES Sri Nuryati; Fauzan Wahib Alsani; Hasan Nasrullah; Odang Carman; Yuni Puji Hastuti; Eni Kusrini; Alimuddin Alimuddin
Indonesian Aquaculture Journal Vol 15, No 1 (2020): (June, 2020)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.15.1.2020.25-32

Vaccination is a practical step in preventing diseases caused by koi herpes virus (KHV) in koi fish (Cyprinus carpio haematopterus). We have developed two DNA vaccines for KHV named as GP-25 and GP-11 from two local isolates coded as ORF25 and ORF81, respectively. Although both vaccines have been reported to increase survival rates, the evaluation of koi fish immune responses at the molecular level has not been done post-vaccinations. The aim of this research was to determine the effects of koi herpesvirus DNA vaccine on the immune-modulation of koi fish at mRNA level. This recent research used the best vaccine doses of both vaccines determined from our previous study: 7.5 and 12.5 µg per 100 g fish of GP-11, and 12.5 µg per 100 g fish of GP-25. The immune gene expression was analyzed using the RT-qPCR method from the fish liver at 0, 1, 7, 14, and 28 days post-vaccination (dpv). The results showed that, in the vaccinated fish, the immune genes viz. tumor necrosis factor a (TNFa), interleukin-1b (IL1b), interferon-g (IFNg), Mx1, immunoglobulin Mu chain (IgM), and major histocompatibility complex (MHC) class I and class II were induced to significant extents. The higher dose vaccination using the GP-11 vaccine showed higher immune gene expression than that of the lower dose. Furthermore, the GP-25 vaccine had induced lower immune responses than the GP-11 vaccine when using the same dose of vaccination, but relatively the same when the half-dose of GP-11 vaccine was used. In conclusion, the GP-11 and GP-25 vaccine provided the immune-modulatory effects on the koi fish immune response after vaccination.

KLONING cDNA HORMON PERTUMBUHAN DARI IKAN GURAME (Osphronemus gouramy) Estu Nugroho; Alimuddin Alimuddin; Anang Hari Kristanto; Odang Carman; Novi Megawati; Komar Sumantadinata
Jurnal Riset Akuakultur Vol 3, No 2 (2008): (Agustus 2008)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Penelitian mengenai kloning cDNA pengkode hormon pertumbuhan ikan gurame telah dilakukan. Tujuan dari penelitian ini adalah untuk memperoleh sekuens DNA komplemen hormon pertumbuhan sebagai langkah awal dalam rangka pengembangan teknologi rekayasa genetik ikan gurame. Empat buah kelenjar hifopisa ikan gurame digunakan sebagai bahan bakunya dan dilakukan proses ekstraksi RNA total dari kelenjar hipofisa, dilanjutkan dengan sintesis cDNA, amplifikasi PCR, purifikasi fragmen DNA dari gel, ligasi produk PCR dengan vektor kloning, transformasi dan inkubasi bakteri, seleksi koloni bakteri putih, isolasi plasmid, dan sekuensing. Hasil sekuensing menunjukkan bahwa panjang produk amplifikasi PCR adalah 843 bp yang menyandikan 204 asam amino residu dan mengandung sekuens-sekuens yang konserf untuk gen hormon pertumbuhan (GH). Analisis homologi menunjukkan kesamaan sekuens hasil isolasi antara 52,4%--97,6% dengan gen GH ikan lainnya, dengan persentase homologi tertinggi adalah dengan ikan sepat. Dengan demikian dapat disimpulkan bahwa sekuens hasil isolasi merupakan sekuens gen GH. Dari hasil analisis sekuens terlihat bahwa gen GH ikan gurame secara evolusi adalah konserf.Research on cDNA cloning encoded the gouramy growth hormone was conducted. The aim of the research was to get complementary DNA, cDNA, sequences of growth hormone as an initial step to develop genetic engineering of gouramy fish. Four pituitary glands of the gouramy were taken and then processed with total RNA extraction, and continued with cDNA synthesis, PCR amplification, DNA fragment purification from the gel, PCR product legation with cloning vector, transformation and incubation of bacteria, white colony bacteria selection, plasmid isolation and sequencing analysis. Sequencing result showed that the amplified PCR product length had 834 bp, encoding 204 amino acid residue and contained conserve sequence for GH (growth hormone) gen. Homolog analysis showed sequence similarity of isolated result between 52.4%—97.6% with other GHs with the highest percentage resulted from the Trichogaster pectoralis. From the sequence analysis showed that GH gen of gouramy at an evolutionary manner was conserve.

RAGAM GENOTIPE IKAN TENGADAK, Barbonymus schwanenfeldii (Bleeker 1854) PERSILANGAN POPULASI JAWA DAN KALIMANTAN BERDASARKAN RAPD Deni Radona; Dinar Tri Soelistyowati; Rudhy Gustiano; Odang Carman; Irin Iriana Kusmini; Sri Sundari
Jurnal Riset Akuakultur Vol 11, No 2 (2016): (Juni 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Dalam rangka pengelolaan sumber genetik untuk pengembangan budidaya ikan tengadak maka perlu dilakukan evaluasi sumber genetik ikan tengadak asal Jawa dan Kalimantan, serta progeni hibridanya. Analisis genetik dilakukan secara molekuler dengan metode RAPD. Jumlah sampel yang digunakan untuk analisis RAPD sebanyak 10 ekor setiap populasi. Spesimen yang digunakan untuk analisis RAPD adalah sirip untuk induk dan whole body untuk hibrida. Hasil menunjukkan polimorfisme (32,43%) dan heterozigositas (0,13) tertinggi terdapat pada ikan tengadak hasil persilangan betina Jawa x jantan Kalimantan, sedangkan yang terendah diperoleh pada persilangan betina Kalimantan x jantan Jawa (polimorfisme: 21,62% dan heterozigositas: 0,10). Berdasarkan dendrogram hubungan kekerabatan interpopulasi ikan tengadak hasil persilangan (betina Kalimantan x jantan Jawa) dengan induknya (populasi Jawa dan Kalimantan) menggunakan tiga primer RAPD (OPA-08, OPA-09, dan OPC-02) menunjukkan jarak genetik berkisar 0,48. Ikan tengadak betina asal Jawa dan jantan asal Kalimantan potensial meningkatkan keragaman genetik.In order to maintain the genetic sources of tinfoil barb for aquaculture development, it is necessary to evaluate the genetic diversity crossbred results of tinfoil barb from Java and Kalimantan. The genetic assessment was conducted by genotype trails using RAPD methods. The samples used for the analysis of RAPD was as much as 10 individuals. Specimens used for RAPD analysis was a fin for broodstock and whole body for the hybrid fish. The result showed that the highest polymorphism and heterozygosity were found 32.43% and 0.13 in population crossbred of female Java x male Kalimantan. While the lowest polymorphism and heterozygosity were detected on population of f Kalimantan x m Java (21.62% and 0.10, respectively). Based on the relationship between tinfoil barb hybrid (female Kalimantan x male Java) with a both broodstock population using three RAPD primers (OPA-08, OPA-09, and OPC-02) resulted in genetic distance of 0.48. Females tinfoil barb from Kalimantan and males from Java potential could increase genetic diversity.

PERFORMA IKAN NILA (Oreochromis niloticus) HASIL SEX REVERSAL, GENETICALLY MALE DAN YY PADA FASE PENDEDERAN PERTAMA Odang Carman; Aulia Saputra; Alimuddin Alimuddin; Maskur Maskur; Dian R Herdianto; Ratu Siti Aliah; Komar Sumantadinata; Tristiana Yuniarti
Jurnal Riset Akuakultur Vol 4, No 1 (2009): (April 2009)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Penelitian ini dilakukan untuk mengkaji performa ikan nila hasil sex reversal (SRV), genetically male tilapia (GMT), dan YY pada fase pendederan pertama di akuarium. Benih ikan dipelihara selama 22 hari, dari umur 6 hari hingga 28 hari. Parameter yang diamati meliputi tingkat sintasan, persentase ikan jantan, laju pertumbuhan, dan biomassa. Hasil penelitian menunjukkan bahwa tingkat sintasan tidak berbeda (P>0,05) antar ketiga kelompok ikan dan kontrol (KN), berkisar antara 85,30%--86,20%. Persentase ikan jantan antara SRV (94,5% ± 1,32%) vs. GMT (93,8% ± 1,25%) dan GMT vs. YY (90,2% ± 1,83%) tidak berbeda (P>0,05), sedangkan antara SRV lebih tinggi daripada YY (P<0,05). Persentase ikan jantan pada ketiga kelompok ikan tersebut lebih tinggi (P<0,05) dibandingkan dengan KN (56,9% ± 3,62%). Pertumbuhan ikan YY dan GMT lebih cepat (P<0,05) dibandingkan dengan ikan SRV dan kontrol (KN). Bobot rata-rata ikan YY pada akhir penelitian mencapai 485 mg, ikan GMT 456 mg, ikan SRV 379 mg dan kontrol 342 mg. Produksi biomassa ikan YY, GMT, dan SRV masing-masing sebesar 41,3%; 32,9%; dan 10,3% lebih tinggi dibandingkan dengan KN. Dengan performa yang tinggi dan pertimbangan teknis di lapangan, benih GMT merupakan alternatif yang baik untuk dibudidayakan dalam rangka meningkatkan produksi ikan nila.The experiment was conducted to determine the performance of sex reversed (SRV), genetically male tilapia (GMT), and YY tilapia on first nursery phase in aquarium. Fry were reared for 22 days, from 6 to 28 days-old. Survival rate, percentage of male fish, growth rate and biomass were observed. The result of the study showed that survival rate among fish group and control were similar (P>0.05), ranged from 85.30%-86.20%. Percentage of male fish between SRV (94.5% ± 1.32%) versus GMT (93.8% ± 1.25%) and GMT versus YY (90.2% ± 1.83%) were also similar (P>0.05), while SRV is higher than YY (P<0.05). Percentage of male fish in the three fish groups was higher than that of control (56.9% ± 3.62%). Growth of YY fish and GMT were higher compared to SRV and control fish (KN). The mean weight of YY fish at the end of the experiment reached 476 mg, GMT fish 447 mg, SRV fish 379 mg and control 342 mg. Biomass of YY, GMT and SRV fish were respectively higher by 41.3%, 32.9%, and 10.3% compared to control. With high performance and technical consideration in farm, GMT fish can be a potential alternative to be cultured in fish farm in order to increase aquaculture production of nile tilapia.

RESPONS PERTUMBUHAN DAN EKSPRESI GEN UDANG VANAME, Litopenaeus vannamei SETELAH DIRENDAM DALAM LARUTAN HORMON PERTUMBUHAN REKOMBINAN IKAN KERAPU KERTANG Siti Subaidah; Odang Carman; Komar Sumantadinata; Sukenda Sukenda; Alimuddin Alimuddin
Jurnal Riset Akuakultur Vol 7, No 3 (2012): (Desember 2012)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Pertumbuhan ikan dapat ditingkatkan menggunakan hormon pertumbuhan rekombinan. Penelitian ini bertujuan mengkaji respons pertumbuhan dan ekspresi gen udang vaname, Litopenaeus vannamei setelah direndam dengan hormon pertumbuhan rekombinan ikan kerapu kertang, Epinephelus lanceolatus (rElGH). Pada percobaan pertama, post larva stadia 2 (PL-2) sebanyak 1.500 ekor direndam selama 1 jam dalam 1 liter air laut yang mengandung rElGH lima dosis berbeda, yaitu 150; 15; 1,5; 0,15; dan 0,015 mg/L dan bovine serum albumin 0,01%. Setiap perlakuan diulang 3 kali. Perendaman dilakukan dalam kantong plastik ditambah oksigen (volume air :oksigen = 1:5). Udang dipelihara dalam akuarium volume 60 liter dengan kepadatan 25 ekor/litersampai PL-14. Hasil penelitian menunjukkan bahwa dosis 15 mg/L memberikan peningkatan bobot badan, panjang badan, dan sintasan tertinggi (P<0,05) masing-masing sebesar 37,77%; 12,75%; dan 9,45% dibandingkan dengan kontrol. Ekspresi mRNA single insulin binding domain (SIBD) pada PL-14 yang dianalisis dengan realtime PCR menunjukkan kenaikan sebesar 3,3 kali pada udang yang direndam rElGH dibandingkan dengan kontrol, dan dapat dinyatakan bahwa SIBD berperan penting dalam induksi pertumbuhan. Tingkat ekspresi moult inhibiting hormone meningkat sekitar 13%, sedangkan ekspresi cyclopilin A pada udang yang direndam rElGH sama dengan kontrol. Pada percobaan kedua, perendaman PL-2 dalam larutan rElGH dosis 15 mg/L dengan lama waktu 3 jam meningkatkan bobot badan sebesar 62,18% lebih tinggi daripada perendaman 1 jam. Dengan demikian, perendaman udang dalam larutan rElGH meningkatkan pertumbuhan dan ekspresi gen SIBD, dan metode ini dapat berguna dalam peningkatan produksi budidaya.

PEMBENTUKAN POPULASI SINTETIS UNTUK PENINGKATAN KUALITAS GENETIK IKAN MAS Didik Ariyanto; Odang Carman; Dinar Tri Soelistyowati; Muhammad Zairin Junior; M. Syukur; Yogi Himawan; Flandrianto S. Palimirmo
Jurnal Riset Akuakultur Vol 16, No 2 (2021): (Juni, 2021)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Benih ikan mas telah mengalami penurunan kualitas genetik yang menyebabkan penurunan performa fenotipik di lingkungan budidaya. Salah satu upaya perbaikan genetik adalah melalui pembentukan populasi sintetis yang merupakan penggabungan potensi genetik beberapa populasi plasma nutfah ikan mas. Penelitian ini bertujuan membentuk dan mengevaluasi performa genotipik dan fenotipik populasi sintetis ikan mas, yang merupakan penggabungan dari strain Rajadanu, Majalaya, Sutisna, Wildan, dan Sinyonya. Performa genotipik dievaluasi menggunakan metode mikrosatelit DNA, sedangkan performa fenotipik dievaluasi menggunakan analisis biometrik terkait kegiatan budidaya. Hasil penelitian menunjukkan bahwa nilai keragaman genetik populasi sintetis lebih tinggi 55,0%-287,5% dengan tingkat inbreeding 40,0%-77,14% lebih rendah dibanding populasi-populasi pembentuknya. Hal ini berdampak terhadap performa fenotipik populasi sintetis yang lebih baik, diindikasikan dengan peningkatan panjang, bobot akhir, dan tingkat produktivitas, masing-masing sebesar 2,5%-20,6%; 9,4%-61,8%; dan 18,2%-66,0% lebih baik dibanding populasi-populasi pembentuknya. Peningkatan kualitas genetik dan performa fenotipik populasi sintetis ini memberikan peluang untuk memperbaiki kualitas benih ikan mas pada kegiatan budidaya.Common carp in Indonesia has experienced a decline in genetic quality. The progressive decline leads to a significant decrease in carp performance in the farming environment. One of the efforts to genetically improve carp growth performance is through developing synthetic carp populations, which is a blend of the genetic potentials from several germplasm populations. This study aimed to form and evaluate the performance of genotypic and phenotypic of synthetic populations of common carp, blended from five strains of common carp, i.e., Rajadanu, Majalaya, Sutisna, Wildan, and Sinyonya. The genotypic performance was evaluated using the DNA microsatellite method. The phenotypic performance was assessed using biometric analysis, especially in terms of culture performance. The results showed that the genotypic performance of the synthetic populations of common carp was better than that of the founder strains. This performance was indicated by higher genetic diversity values, about 55.0%-287.5% and lower levels of inbreeding, about 40.0%-77.1%, compared with their founder populations. Phenotypic performance of the synthetic populations is also better than their founder populations, indicated by higher body length, weight, and productivity, about 2.5%-20.6%, 9.4%-61.8%, and 18.2%-66.0%, respectively. The improvement on genetic quality and phenotypic performance of the synthetic population provide opportunities to improve the quality of common carp fry in aquaculture activity.

RESPONS PERTUMBUHAN DAN EFISIENSI PAKAN PADA IKAN NILA UKURAN BERBEDA YANG DIBERI PAKAN MENGANDUNG HORMON PERTUMBUHAN REKOMBINAN Muhammad Muhammad; Alimuddin Alimuddin; Muhammad Zairin Junior; Odang Carman
Jurnal Riset Akuakultur Vol 9, No 3 (2014): (Desember 2014)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Penelitian ini bertujuan untuk menguji respons pertumbuhan dan pemanfaatan pakan pada ikan nila (Oreochromis niloticus) strain SULTANA (seleksi unggul Selabintana) ukuran berbeda yang diberi pakan mengandung hormon pertumbuhan rekombinan ikan kerapu kertang, Epinephelus lanceolatus (rElGH). Ikan dengan ukuran awal 3,5± 0,3 g (perlakuan A); 12,5±0,4 g (perlakuan B); dan 40±2,4 g (perlakuan C) dipelihara dalam hapa (2 m x 1 m x 1 m) yang dipasang di kolam beton (20 m x 10 m x 1,5 m) pada padat tebar 50 ekor ikan/hapa. Setiap perlakuan diberi pakan mengandung 3 mg rElGH/kg, dan tidak diberi rElGH (kontrol; K). Masing-masing perlakuan terdiri atas tiga ulangan. Ikan dipelihara selama delapan minggu. Hasil penelitian menunjukkan bahwa rata-rata pertambahan biomassa (ΔB) ikan perlakuan rElGH adalah lebih tinggi (P<0,05) dibandingkan dengan kontrol, sedangkan perlakuan ukuran ikan tidak berpengaruh nyata (P>0,05). Laju pertumbuhan harian ikan perlakuan rElGH lebih tinggi (P<0,05) dibandingkan dengan kontrol. Perlakuan B memiliki pertambahan bobot relatif terhadap kontrolnya (19,9%) lebih tinggi daripada perlakuan A (10,5%) dan C (5,6%). Sintasan ikan perlakuan dan kontrol adalah sama (P>0,05), berkisar 90,7%-96,7%. Konversi pakan pada ikan perlakuan rElGH lebih rendah (P<0,05) dibandingkan dengan kontrol, kecuali perlakuan C. Kadar glikogen hati dan otot, retensi protein dan lemak, indeks hepatosomatik, dan rasio RNA : DNA ikan perlakuan rElGH lebih tinggi daripada kontrol. Dengan demikian, pemberian rElGH meningkatkan pertumbuhan dan efisiensi pakan untuk pertumbuhan ikan nila, dan hal ini berpotensi tinggi diterapkan untuk meningkatkan produksi dan efisiensi budidaya ikan nila.

KARAKTERISTIK FENOTIPE DAN GENOTIPE LIMA STRAIN IKAN MAS DI JAWA BARAT DAN BANTEN Didik Ariyanto; Odang Carman; Dinar Tri Soelistyowati; Muhammad Zairin Jr.; Muhamad Syukur
Jurnal Riset Akuakultur Vol 13, No 2 (2018): (Juni, 2018)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Langkah awal program pemuliaan adalah koleksi dan pengenalan karakter materi pemuliaan tersebut. Hasil karakterisasi digunakan sebagai dasar pertimbangan metode pelaksanaan program pemuliaan yang akan dilakukan. Koleksi material genetik untuk program pemuliaan ikan mas menghasilkan lima strain yang dominan dibudidaya di wilayah Jawa Barat dan Banten, yakni strain Rajadanu, Sutisna, Majalaya, Wildan, dan Sinyonya. Pengenalan karakter material genetik ikan mas hasil koleksi dilakukan melalui dua pendekatan, yaitu fenotipe menggunakan metode truss morfometrik dan genotipe menggunakan metode mikrosatelit DNA. Hasil analisis menunjukkan bahwa variasi keragaan fenotipe kelima strain ikan mas relatif sesuai dengan variasi keragaan genotipenya. Selain mengelompokkan antar strain, hasil analisis genotipe juga menunjukkan bahwa tingkat keragaman genetik kelima strain ikan mas yang diindikasikan dengan nilai heterozigositas (Ho) relatif rendah, yaitu berkisar antara 0,08-0,20 dengan jarak genetik antar strain berada dalam kisaran 0,420-0,582.The first step in a fish breeding program is the collection and characterization of the breeding subject. The results of characterization are used as a baseline to select suitable potential methods used in the breeding program. The samples of genetic materials of five strains of common carp (Rajadanu, Sutisna, Majalaya, Wildan, and Sinyonya) were obtained from West Java and Banten Province. The characterization of collected genetic materials of the common carp species followed the phenotype and genotype approaches. Phenotypic characterization used truss morphometric method while genotype characterization applied DNA microsatellite method. The results showed that the phenotypic variation of the common carp had a close fit with its genotypic variation. In addition, the genotype analysis also showed that the genetic diversity level of the strains was relatively low indicated by the narrow ranges of heterozygosity values (Ho) (0.08-0.20) and genetic distance among strains (0.420-0.582).

Co-Authors ', Alimuddin , Alimuddin , Rahman, , . Syafiuddin Abinawanto Abinawanto Agus Oman Sudrajat Ahmad Teduh Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Amalia Hayati Anang Hari Kristanto Andi Aliah Hidayani Andri Iskandar Angraini, Siti Rena Yulia ANNA OCTAVERA Astri Ayuningtias Aulia Saputra Aulia Saputra Avarre, Jean-Christophe Ayi Santika Budyansah Siregar Cahyono, Tatak Dwi Cecilia Eny Indriastuti Chairul Muluk Ching-Fong Chang D.S. Sjafei Dede Hermawan DEDI JUSADI Dendi Hidayatullah, Dendi Deni Radona Deni Radona Deni Radona Deni Radona, Deni Dian Eka Ramadhani Dian R Herdianto Didik Ariyanto Didik Ariyanto Dinar Tri Soelistyowati Dita Puji Laksana Djamhuriyah S. Said Eddy Supriyono Eka Haris Nurfauzi Elfrida Ratnawati Enang Harris Eni Kusrini Estu Nugroho Eva Ayuzar Fajar Maulana . Fauzan Wahib Alsani Firda Amalia Sukma Firmansyah, Rodhi Flandrianto S. Palimirmo Giri Maruto Darmawangsa Goro Yoshizaki Goro Yoshizaki GORO YOSHIZAKI Guan-Chung Wu Handika Gilang Pramana Putra Harton Arfah Hasan Nasrullah Hendriana, Andri Herjayanto, Muh. Hidayat Hidayat Hidayatush Sholihin Hylda Khairah Putri, Hylda Khairah I. Andriani I. Herviani Ibrahim Satrio Faqih Ibrahim, Yusran Iis Diatin Indra Lesmana Irin Iriana Kusmini IRMA ANDRIYANI Irvan Faizal Irvan Faizal Irvan Faizal Irwan Irwan Iskandar, Andri Jasmadi Jasmadi K. Sumantadinata Kadarusman . Kaliky, Nunun Ainun Putri Sari Banun Kesit Tisna Wibowo Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Kukuh Adiyana, Kukuh Laurent Pouyaud Lies Emmawati Hadie Lies Emmawati Hadie Lilis Nurjannah Lina Mulyani Lisa Ruliaty Lola Irma Purwanti M. Syaifudin M. Syukur M. Zairin Junior Maharani, Adinda Marlina Achmad Maskur Maskur MH. Fariduddin Ath-thar Mia Setiawati Moh. Abduh Nurhidayat Mubinun Mubinun Muh. Herjayanto Muhamad Fathurrizki Darmawan Muhamad Syukur Muhammad Agus Muljanto MUHAMMAD AGUS SUPRAYUDI Muhammad Fakhri Muhammad Muhammad Muhammad Rafi Muhammad Sami Daryanto Muhammad Zairin Muhammad Zairin Muhammad Zairin Jr Muhammad Zairin Jr. Muslim Muslim N. Sugiri Nadia Mega Aryani, Nadia Mega NM Fatih Asror AM Novi Megawati Nugraha, Media Fitri Isma Nur Bambang Priyo Utomo Prama Hartami Prama Hartami Raden Roro Sri Pudji Sinarni Dewi Rahma Vida Anandasari, Rahma Vida Rahman Rahman Rahmat Hidayat Rahmat Hidayat Rahmi, Kurnia Anggraini Ratu Siti Aliah Rezki, Dinda Wahyu RIDWAN AFFANDI Ronny Rachman Noor Rudhy Gustiano Rudhy Gustiano S. Purwati Salamah Salamah Salsabilla Galby Fataya Samalei, Ermianus Siti Subaidah Siti Subaidah Sri Nuryati Sri Setyo Wulandari, Sri Setyo Sri Sundari Subandriyo Subandriyo Sukenda . Sukenda Sukenda Sumie Etoh Syahril, Alfis Tatang Juanda Toshio Takeuchi Tristiana Yuniarti Wartono Hadie Wartono Hadie Wasmen Manalu Wedaraningtyas Nugrahani Wicaksono, Aryo Wenang Yogi Himawan Yumaidawati, Nurfitriani Siti Yuni Puji Hastuti Zairin Jr., Muhammad

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