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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Agromet Forum Pasca Sarjana Jurnal Ilmu Pertanian Jurnal Ilmiah Aplikasi Isotop Radiasi JURNAL SAINS DAN TEKNOLOGI INDONESIA Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Iptek Tanaman Pangan Buletin Plasma Nutfah AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Widyariset Planta Tropika BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal AGROSAINS dan TEKNOLOGI Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Jurnal Agrotek Tropika Jurnal Agrotech Journal of Tropical Crop Science Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) jurnal Ilmu Pertanian (Agricultural Science) JURNAL PANGAN Jurnal Penelitian Tanaman Industri (Littri) Widyariset Makara Journal of Science
Didy Sopandie
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
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Journal : Journal of Tropical Crop Science

 1 
Cloning and Characterization of Partial Chlorophyll a Oxygenase (CAO) Gene Involved in Soybean Shade Tolerance Mechanism Khumaida, Nurul; Kisman, Kisman; Sopandie, Didy
Journal of Tropical Crop Science Vol 2 No 2 (2015): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2936.921 KB) | DOI: 10.29244/jtcs.2.2.1-4

Abstract

Plant tolerance mechanism under shade stress is indicated by high chlorophyll-b content, which is synthesized from chlorophyll a by chlorophyll a-oxygenase (CAO) gene. The study was aimed to clone and characterize the partial of CAO gene involved in shade tolerance mechanism in soybean.  Total RNA was isolated from the second trifoliate leaves of soybean plants and the first strand cDNA was prepared using Reverse Transcriptase Moloney Murine Leukemia Virus (RT-M-MLV) (RNase H-). Northern blot hybridization was used to analyze expression of partial CAO gene. The result showed that one partial gene, CAO 3-4 (1,052 bp), comprised of 292 adenine (a), 241 cytosine (c), 276 guanine (g) and 242 thymine (t), therefore the number of amino acid deduction was 338.  The expression of CAO 3-4 partial gene was higher in shade tolerant genotypes than in shade sensitive genotypes, both under 50% shade and dark. These results demonstrated that the CAO gene is involved in shade tolerance mechanism of soybean.
Promoting Sustainable Sorghum Production: The Role of Ratoon Cultivation and Fertilizer Management Mahpuzah, Marfiatun; Sopandie, Didy; Wirnas, Desta; Trikoesoemaningtyas
Journal of Tropical Crop Science Vol. 12 No. 01 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.01.47-58

Abstract

This study investigated the biomass productivity of sorghum main crop, first ratoon, and second ratoon. A randomized complete block design was employed for the main crop experiment, with eight sorghum genotypes (NS2-19, NS2-102, NS2-109, NS2-111, NS2-140, IPB 4, IPB 8, and “Numbu”) as the treatment factor. The ratoon crop was designed using a Split Plots Design with two treatment factors and three replications. The first factor was fertilizer treatment on the ratoon crops, with two levels: complete N, P, and K fertilizer (urea 133 kg.ha-1, KCl 100 kg.ha-1, and SP-36 100 kg.ha-1) and N fertilizer only (urea 133 kg.ha-1); this factor was arranged as the main plot. The second factor comprised eight elite sorghum lines, the same as those used in the main crop experiment, arranged as subplots. The results indicated that complete NPK fertilization and N fertilizer alone produced similar biomass productivity in sorghum ratoon crops. Among the genotypes, NS2-140 demonstrated the highest biomass productivity in the main crop, NS2-109 in the first ratoon, and NS2-19 in the second ratoon. The second ratoon exhibited the highest biomass yield compared to the main crop and the first ratoon.
Plant Growth, Nitrogen Content, Nitrogen Uptake, and Seed Quality in Six Bambara Groundnut (Vigna subterranea (L.) Verdc) Landraces Rosyad, Astryani; Ilyas, Satriyas; Qadir, Abdul; Suhartanto, M Rahmad; Sopandie, Didy
Journal of Tropical Crop Science Vol. 12 No. 03 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.03.572-583

Abstract

Bambara groundnut (Vigna subterranea (L.) Verdc) is recognized for its nutritional benefits and ability to improve soil fertility through nitrogen fixation. This study evaluated growth performance, nitrogen dynamics, and seed quality across six landraces: Tasikmalaya, Sukabumi, Sumedang, Small Sumedang, Bogor, and Gresik. The experiment was conducted at IPB University’s Sawah Baru experimental field from July 2022 to March 2023. The experiment used a randomized complete block design with four replications. The measured variables were petiole length, number of petioles, number of leaves, leaf greenness, plant dry weight, nitrogen content, nitrogen uptake in roots, petioles, leaves, and pods, protein content in pods, and seed quality. Results showed significant variation among landraces. Gresik exhibited superior vegetative growth and dry biomass, while Tasikmalaya had the highest pod nitrogen (4.58%) and protein content (28.03%), indicating efficient nitrogen assimilation. Nitrogen analysis revealed distinct uptake patterns across landraces, particularly with Gresik, where a rapid increase in nitrogen suggests that this landrace is well-suited to environments where nitrogen is efficiently fixed and mobilized toward reproductive growth. Sukabumi and Bogor showed the highest germination rates (94%), and Bogor and Gresik had the highest vigor indices (76% and 72%). Overall, Gresik is recommended for balanced performance in growth and seed vigor, while Tasikmalaya is optimal for protein yield and nitrogen use efficiency.
Co-Authors , Hamin , Kisman , Purwono , Suharsono , Syafruddin , Tasliah . SUHARSONO . SUWARNO A. HAITAMI A.A. Ketut Agung Cahyawan W Abd. Aziz Syarif ABDUL KARIM MAKARIM Abdul Munif Abdul Qadir Adolf Pieter Lontoh, Adolf Pieter Agus Purwito AHMAD JUNAEDI AKIHO YOKOTA Akiho Yokota AKIHO YOKOTA Aksarah Pas, Aris Alce Ilona Noyaa Alifiya Herwitarahman Amris Makmur Amrullah Amrullah Anas D Susila Anas Dinurrohman Susila Angelita Puji Lestari ANGELITA PUJI LESTARI Annisa Fadila Ardie, and Sintho Wahyuning Ardie, dan Sintho Wahyuning Arvita Netti Sihaloho, Arvita Netti ASMARLAILI SAHAR Atang Sutandi Azri Kusuma Dewi Azri Kusuma Dewi Budi Mulyanto Careca Sepdihan Rahmat Hidayatullah Chairani Hanum Chairani Hanum Christoph Leuschner Deden Derajat Matra Desta Wirnas Dhika Prita Hapsari DWI ANDREAS SANTOSA Edi Santosa Edi Santosa Edi Santosa Efendi, Darda Enny Sudarmonowati Enny Sudarmonowati Enung Sri Mulyaningsih Enung Sri Mulyaningsih Eny Widajati Erma Prihastanti Fadliah Salim Faqih Udin GUSMAINI GUSMAINI Gusmaini Gusmaini GUSMAINI GUSMAINI, GUSMAINI HAJRIAL ASWIDINNOOR Hajrial Aswidinoor Halimatus Syahdia Hasibuan Hamim Hamim Hanedi Darmasetiawan HANNA ARTUTI EKAMAWANTI Hapsoh Hidayah, Agus Nur I Ketut Suada IBNUL QAYIM Ida Hanarida Soemantri Ika Mariska Ika Mariska Ika Mariska Ilona Noyaa, Alce Imam Widodo Inez Hortense Slamet Loedin Inez Hortense Slamet Loedin Inez Hortense Slamet-Loedin INEZ HORTENSE SLAMET-LOEDIN Iskandar Lubis Joko Prasetiyono Joko Prasetiyono Juang Gema Kartika K. Idris Karlin Agustina Kartika Ning Tyas Ketty Suketi KINYA AKASHI KINYA AKASHI Kisman Kisman Komaruddin Idris Komaruddin Idris La Muhuria Laela Sari Laela Sari laela Sari, laela Lukman Chakim Luluk Setyaningsih Lutfy Ditya Cahyanti, Lutfy Ditya M A Chozin M. A. Chozin Maemonah, Maemonah Mahpuzah, Marfiatun Mariska, Ika Masdiar Bustamam Masdiar Bustamam Masumi Moritsugu Matra, Deden Derajat Maya Melati Mayang Sari Meliala, Merry Gloria Moh. Yani Momongan, Jorex Daniel Muhamad Syukur Muhammad Arif Yudiarto Muhammad Jusuf Munarti Munif Ghulamahdi Muzuni, Muzuni Nani Heryani Nasution, Annisa Andrini Ni Made Armini Wiendi NURLIANI BERMAWIE Nurliani Bermawie Nurliani Bermawie Nurul Khumaida Osmat Azzam Jefferson Osmat Azzam Jefferson, Osmat Azzam OuwerkerV, Pieter BF Pieter B.F. Ouwerkerk Pieter BF OuwerkerV Putri Andini Mandasari RADITE TISTAMA Ragapadmi Purnamaningsih RAGAPADMI PURNAMANINGSIH Ratna Yuniati Rini, Erin Puspita Roedhy Poerwanto Rossa Yunita Rossa Yunita Rossa Yunita Rosyad, Astryani S. Suharsono Saepudin, Adam Sandi, Yusri Iryas Sandra Arifin Aziz Saragih, Ery Leonardo Sari, Laela Sarsidi Sastrosumarjo SATRIYAS ILYAS Satya Nugroho Sinaga, Falencia Sinaga, Parlin H. Sintho Wahyuning Ardie Siti Marwiyah Slamet Loedin, Inez Hortense Sobir Sobir SOEKISMAN TJITROSEMITO Soeranto Human Sri Mulatsih SRI RAHAYU Sri Setyati Harjadi Sri Wilarso Budi Sudarmonowati, Enny Sudirman Yahya Sudirman Yahya Sugianta Sugianta Sugianta, Sugianta SUGIONO MOELJOPAWIRO Sugiono Moeljopawiro Suharsono . Suharsono Suharsono Suharsono Suharsono Suhartanto, Muhammad Rahmad Sumiati, dan Sungkono Sungkono Supijatno Surjono Hadi Sutjahjo Suwarno Suwarno Suwarno Suwarno Suwarto Suwarto Syamsidah Rahmawati Syamsidah Rahmawati, Syamsidah T. Kawasaki T. M. H. Oelim Tanari, Yulinda Tintin Suhartini Titin Handayani Toshio Kawasaki Toyip Toyip TRI KOESOEMANINGTYAS Tri Lestari Trikoesoemaningtyas Utami, Nita Nur UTUT WIDYASTUTI Utut Widyastuti UTUT WIDYASTUTI Utut Widyastuti Suharsono Wage Ratna Rohaeni Wahju Q. Mugnisjah Wahju Qamara Mugnisjah YADI SETIADI Yadi Setiadi Yadi Setiadi Yadi Setiadi Yonny Koesmaryono Yopie Moelyohadi Yuli Sulistyowati YULIN LESTARI Yuniati, Ratna Yunita, Rossa