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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Agromet Forum Pasca Sarjana Jurnal Ilmu Pertanian Jurnal Ilmiah Aplikasi Isotop Radiasi JURNAL SAINS DAN TEKNOLOGI INDONESIA Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Iptek Tanaman Pangan Buletin Plasma Nutfah AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Widyariset Planta Tropika BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal AGROSAINS dan TEKNOLOGI Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Jurnal Agrotek Tropika Jurnal Agrotech Journal of Tropical Crop Science Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) jurnal Ilmu Pertanian (Agricultural Science) JURNAL PANGAN Jurnal Penelitian Tanaman Industri (Littri) Widyariset Makara Journal of Science
Didy Sopandie
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
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Journal : Jurnal AgroBiogen

 1 
Identifikasi Marka Polimorfik untuk Pemuliaan Padi Toleran Defisiensi Fosfor Joko Prasetiyono; Hajrial Aswidinoor; Sugiono Moeljopawiro; Didy Sopandie; Masdiar Bustamam
Jurnal AgroBiogen Vol 4, No 2 (2008): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n2.2008.p51-58

Abstract

Information on polymorphismsamong rice parents are very important in ricebreeding for tolerance to phosphorus defficiency. A studywas conducted at the Molecular Biology Laboratory,Indonesian Center Agricultural Biotechnology and GeneticResources (ICABIOGRAD) from October 2006 to July 2007 toidentify polymorphism markers from 6 rice genotypes. Therice genotypes, i.e., Dodokan, Situ Bagendit, Batur, Kasalath,NIL-C443, dan K36-5-1-1 were analyzed for polymorphismsusing 496 SSR markers, which cover the rice genomes.Seven of the 496 markers were used as foreground andrecombinant selection markers, and the rests (489 markers)were used as background selection markers. PCR amplificationswere separated on a 5% polyacrylamide gel andcolored by the silver staining method. Three different markersamong the seven foreground and recombinant selectionmarkers were selected from each crossing, which aretightly linked with Pup1 gene and have a distance less than 5cM. These markers are Dodokan vs Kasalath (RM277, SSR3,RM519), Dodokan vs NIL-C443 (RM277, SSR3, RM519),Dodokan vs K36-5-1-1 (RM277, SSR3, RM519), Situ Bagenditvs Kasalath (RM28102, SSR3, RM519), Situ Bagendit vs NILC443(RM28102, SSR3, RM519), Situ Bagendit vs K36-5-1-1(RM511, SSR3, RM519), Batur vs Kasalath (RM277, RM1261,RM519), Batur vs NIL-C443 (RM277, RM1261, RM519), andBatur vs K36-5-1-1 (RM28102, SSR3). Variations in backgroundselection primers were found in each chromosomeand in each parent combinations. Primers on chromosome4, 5, and 12 showed the lowest polymorphisms; moreprimers are needed for these chromosomes.
Pengaruh Iradiasi Sinar Gama terhadap Pertumbuhan dan Regenerasi Kalus Padi Varietas Ciherang dan Inpari 13 Rossa Yunita; Nurul Khumaida; Didy Sopandie; Ika Mariska
Jurnal AgroBiogen Vol 10, No 3 (2014): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n3.2014.p101-108

Abstract

Effect of gamma irradiation on rice callus dependson the irradiation dose used. Irradiation dose is one of thefactors that affect the genetic changes in the cells of plants.High doses can result in tissue death, meanwhile low doseswill result in abnormal changes in plant phenotype. The levelof sensitivity of a plant tissue against gamma irradiation isdifferent. This study aimed to evaluate the growth andregeneration of callus in various irradiation doses of gammaray. The plant materials used were mature embryos of ricevar. Ciherang and Inpari 13. This study used a completelyrandomized design with gamma irradiation treatment atdoses of 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 Gy. Eachtreatment consisted of 10 replicates with 5 embryogenic calliper bottle. The results showed that the increasing doses ofgamma irradiation increased the percentage of dead callus,inhibited callus growth, and its ability to regenerate. Thehigh percentages of callus of Ciherang and Inpari 13 thatformed green spots and adventitious shoots were mostlyobtained from controls. The percentages decreased atirradiation doses of 10, 20, 30, and 40 Gy. Moreover, the calliof Ciherang and Inpari 13 were not able to form adventitiousshoots at irradiation doses higher than 40 Gy and 30 Gy,respectively.
Optimasi Regenerasi Padi Indica melalui Jalur Organogenesis Rossa Yunita; Nurul Khumaida; Didy Sopandie; Ika Mariska
Jurnal AgroBiogen Vol 13, No 1 (2017): Juni
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v13n1.2017.p35-42

Abstract

Regenerasi tanaman merupakan tahapan penting yang perlu dikuasai sebelum diaplikasikan untuk perakitan varietas unggul secara kultur in vitro. Penelitian bertujuan mengoptimasi teknik regenerasi in vitro beberapa varietas padi indica melalui jalur organogenesis. Percobaan menggunakan Rancangan Acak Lengkap yang disusun secara faktorial. Materi yang dicobakan, yaitu Ciherang, Inpari 13, Inpara 3, Pokkali, dan IR29. Perlakuan yang diberikan untuk induksi kalus organogenik adalah 2,4-D (0, 1, 3, 5, 7 mg/l) + kasein hidrolisat 3 g/l, untuk regenerasi kalus membentuk tunas adventif adalah BA (0, 1, 5 mg/l) + zeatin (0, 0,1, 0,3 mg/l) + prolin 100 mg/l, untuk multiplikasi tunas adalah MS + thidiazuron/TDZ (0, 0,1, 0,3 mg/l) dan untuk perakar-an adalah IBA (0, 1, 2, 3 mg/l). Hasil penelitian menunjukkan media terbaik untuk induksi kalus untuk padi varietas Ciherang, Inpari 13, dan Pokkali adalah MS + 2,4-D 3 mg/l, sedangkan untuk varietas Inpari 3 dan IR 29 adalah MS + 2,4-D 5 mg/l. Media terbaik untuk regenerasi tunas untuk varietas Ciherang adalah BA 5 mg/l + zeatin 0,1 mg/l + prolin 100 mg/l, untuk Inpari 13 adalah MS + BA 3 mg/l + zeatin 0,3 mg/l + prolin 100 mg/l, untuk Inpara 3 adalah BA 5 mg/l + zeatin 0,3 mg/l + prolin 100 mg/l dan untuk Pokkali dan IR29 adalah MS + BA 3 mg/l + zeatin 0,1 mg/l. Media terbaik untuk multiplikasi tunas adalah MS + TDZ 0,3 mg/l dan untuk induksi perakaran adalah MS + IBA 2 mg/l. Setiap tahapan kegiatan pembentukan planlet membutuhkan formulasi media yang berbeda.
Co-Authors , Hamin , Kisman , Purwono , Suharsono , Syafruddin , Tasliah . SUHARSONO . SUWARNO A. HAITAMI A.A. Ketut Agung Cahyawan W Abd. Aziz Syarif ABDUL KARIM MAKARIM Abdul Munif Abdul Qadir Adolf Pieter Lontoh, Adolf Pieter Agus Purwito AHMAD JUNAEDI AKIHO YOKOTA AKIHO YOKOTA Akiho Yokota Aksarah Pas, Aris Alce Ilona Noyaa Alifiya Herwitarahman Amris Makmur Amrullah Amrullah Anas D Susila Anas Dinurrohman Susila ANGELITA PUJI LESTARI Angelita Puji Lestari Annisa Fadila Ardie, and Sintho Wahyuning Ardie, dan Sintho Wahyuning Arvita Netti Sihaloho, Arvita Netti ASMARLAILI SAHAR Atang Sutandi Azri Kusuma Dewi Azri Kusuma Dewi Budi Mulyanto Careca Sepdihan Rahmat Hidayatullah Chairani Hanum Chairani Hanum Christoph Leuschner Deden Derajat Matra Desta Wirnas Dhika Prita Hapsari DWI ANDREAS SANTOSA Edi Santosa Edi Santosa Edi Santosa Efendi, Darda Enny Sudarmonowati Enny Sudarmonowati Enung Sri Mulyaningsih Enung Sri Mulyaningsih Eny Widajati Erma Prihastanti Fadliah Salim Faqih Udin Gusmaini Gusmaini GUSMAINI GUSMAINI GUSMAINI GUSMAINI, GUSMAINI HAJRIAL ASWIDINNOOR Hajrial Aswidinoor Halimatus Syahdia Hasibuan Hamim Hamim Hanedi Darmasetiawan HANNA ARTUTI EKAMAWANTI Hapsoh Hidayah, Agus Nur I Ketut Suada IBNUL QAYIM Ida Hanarida Soemantri Ika Mariska Ika Mariska Ika Mariska Ilona Noyaa, Alce Imam Widodo Inez Hortense Slamet Loedin Inez Hortense Slamet Loedin Inez Hortense Slamet-Loedin INEZ HORTENSE SLAMET-LOEDIN Iskandar Lubis Joko Prasetiyono Joko Prasetiyono Juang Gema Kartika K. Idris Karlin Agustina Kartika Ning Tyas Ketty Suketi KINYA AKASHI KINYA AKASHI Kisman Kisman Komaruddin Idris Komaruddin Idris La Muhuria Laela Sari Laela Sari laela Sari, laela Lukman Chakim Luluk Setyaningsih Lutfy Ditya Cahyanti, Lutfy Ditya M A Chozin M. A. Chozin Maemonah, Maemonah Mahpuzah, Marfiatun Mariska, Ika Masdiar Bustamam Masdiar Bustamam Masumi Moritsugu Matra, Deden Derajat Maya Melati Mayang Sari Meliala, Merry Gloria Moh. Yani Momongan, Jorex Daniel Muhamad Syukur Muhammad Arif Yudiarto Muhammad Jusuf Munarti Munif Ghulamahdi Muzuni, Muzuni Nani Heryani Nasution, Annisa Andrini Ni Made Armini Wiendi Nurliani Bermawie Nurliani Bermawie NURLIANI BERMAWIE Nurul Khumaida Osmat Azzam Jefferson Osmat Azzam Jefferson, Osmat Azzam OuwerkerV, Pieter BF Pieter B.F. Ouwerkerk Pieter BF OuwerkerV Putri Andini Mandasari RADITE TISTAMA Ragapadmi Purnamaningsih RAGAPADMI PURNAMANINGSIH Ratna Yuniati Rini, Erin Puspita Roedhy Poerwanto Rossa Yunita Rossa Yunita Rossa Yunita Rosyad, Astryani S. Suharsono Saepudin, Adam Sandi, Yusri Iryas Sandra Arifin Aziz Saragih, Ery Leonardo Sari, Laela Sarsidi Sastrosumarjo SATRIYAS ILYAS Satya Nugroho Sinaga, Falencia Sinaga, Parlin H. Sintho Wahyuning Ardie Siti Marwiyah Slamet Loedin, Inez Hortense Sobir Sobir SOEKISMAN TJITROSEMITO Soeranto Human Sri Mulatsih SRI RAHAYU Sri Setyati Harjadi Sri Wilarso Budi Sudarmonowati, Enny Sudirman Yahya Sudirman Yahya Sugianta Sugianta Sugianta, Sugianta SUGIONO MOELJOPAWIRO Sugiono Moeljopawiro Suharsono . Suharsono Suharsono Suharsono Suharsono Suhartanto, Muhammad Rahmad Sumiati, dan Sungkono Sungkono Supijatno Surjono Hadi Sutjahjo Suwarno Suwarno Suwarno Suwarno Suwarto Suwarto Syamsidah Rahmawati Syamsidah Rahmawati, Syamsidah T. Kawasaki T. M. H. Oelim Tanari, Yulinda Tintin Suhartini Titin Handayani Toshio Kawasaki Toyip Toyip TRI KOESOEMANINGTYAS Tri Lestari Trikoesoemaningtyas Utami, Nita Nur UTUT WIDYASTUTI UTUT WIDYASTUTI Utut Widyastuti Utut Widyastuti Suharsono Wage Ratna Rohaeni Wahju Q. Mugnisjah Wahju Qamara Mugnisjah Yadi Setiadi Yadi Setiadi Yadi Setiadi YADI SETIADI Yonny Koesmaryono Yopie Moelyohadi Yuli Sulistyowati YULIN LESTARI Yuniati, Ratna Yunita, Rossa