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Journal : BERITA BIOLOGI

PEWARISAN GEN PENANDA HPT(HYGROMYCINE PHOSPHOTRANSFERASE) BERDASARKAN ANALISIS PCR DAN EKSPRESINYA PADA POPULASI PADI TRANSFORMAN MENGOVEREKSPRESIKAN GEN HD ZIP OSHOX-6 [Segregation of hpt Gene by PCR Analysis and its Expression in Transgenic Rice Population Overexpressing HD-Zip oshox6 Gene] Mulyaningsih, Enung Sri; Aswidinnoor, Hajrial; Sopandie, Didy; OuwerkerV, Pieter BF; Slamet Loedin, Inez Hortense
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2051

Abstract

First generation (TO) transgenic plants do not always segregate their transgenes in a Mendellian segregation pattern. Moreover,instability of heterologous gene expression was often observed in transgenic plants. This phenomenon is often called gene silencing. Gene silencing could happen on different level of gene expression, notably at transcriptional or post-transcriptional level.The purpose of this research was to identify the transgene segregation pattern of a marker gene (hpt) as well as the introduced- regulator gene (OsLEA-oshox6) in second generation (Tl) transgenic rice plants.Gene segregation (hpt) analysis was carried out using PCR method. Gene expression analysis was done by hygromycin antibiotic resistant test of leaf samples.Analysis was carried out on 17 lines of Tl transgenic rice plants from Batutegi and Kasalath cultivars.Mendellian segregation pattern of 3:1 was revealed for all lines based on PCR analysis. Gene expression analysis showed almost all lines was segregated in a Mendellian fashion except for Tl-BT III 2C line. Less transgenic plants that expressed the hpt gene were suggested due to gene silencing effects.It was suggested to happen at transcriptional level.
PEWARISAN GEN PENANDA HPT(HYGROMYCINE PHOSPHOTRANSFERASE) BERDASARKAN ANALISIS PCR DAN EKSPRESINYA PADA POPULASI PADI TRANSFORMAN MENGOVEREKSPRESIKAN GEN HD ZIP OSHOX-6 Enung Sri Mulyaningsih; Hajrial Aswidinnoor; Didy Sopandie; Pieter BF OuwerkerV; Inez Hortense Slamet Loedin
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2051

Abstract

First generation (TO) transgenic plants do not always segregate their transgenes in a Mendellian segregation pattern. Moreover,instability of heterologous gene expression was often observed in transgenic plants. This phenomenon is often called gene silencing. Gene silencing could happen on different level of gene expression, notably at transcriptional or post-transcriptional level.The purpose of this research was to identify the transgene segregation pattern of a marker gene (hpt) as well as the introduced- regulator gene (OsLEA-oshox6) in second generation (Tl) transgenic rice plants.Gene segregation (hpt) analysis was carried out using PCR method. Gene expression analysis was done by hygromycin antibiotic resistant test of leaf samples.Analysis was carried out on 17 lines of Tl transgenic rice plants from Batutegi and Kasalath cultivars.Mendellian segregation pattern of 3:1 was revealed for all lines based on PCR analysis. Gene expression analysis showed almost all lines was segregated in a Mendellian fashion except for Tl-BT III 2C line. Less transgenic plants that expressed the hpt gene were suggested due to gene silencing effects.It was suggested to happen at transcriptional level.
Co-Authors , Hamin , Kisman , Purwono , Suharsono , Syafruddin , Tasliah . SUHARSONO . SUWARNO A. HAITAMI A.A. Ketut Agung Cahyawan W Abd. Aziz Syarif ABDUL KARIM MAKARIM Abdul Munif Abdul Qadir Adolf Pieter Lontoh, Adolf Pieter Agus Purwito AHMAD JUNAEDI Akiho Yokota AKIHO YOKOTA AKIHO YOKOTA Aksarah Pas, Aris Alce Ilona Noyaa Alifiya Herwitarahman Amris Makmur Amrullah Amrullah Anas D Susila Anas Dinurrohman Susila Angelita Puji Lestari ANGELITA PUJI LESTARI Annisa Fadila Ardie, and Sintho Wahyuning Ardie, dan Sintho Wahyuning Arvita Netti Sihaloho, Arvita Netti ASMARLAILI SAHAR Atang Sutandi Azri Kusuma Dewi Azri Kusuma Dewi Budi Mulyanto Careca Sepdihan Rahmat Hidayatullah Chairani Hanum Chairani Hanum Christoph Leuschner Deden Derajat Matra Desta Wirnas Dhika Prita Hapsari DWI ANDREAS SANTOSA Edi Santosa Edi Santosa Edi Santosa Efendi, Darda Enny Sudarmonowati Enny Sudarmonowati Enung Sri Mulyaningsih Enung Sri Mulyaningsih Eny Widajati Erma Prihastanti Fadliah Salim Faqih Udin GUSMAINI GUSMAINI Gusmaini Gusmaini GUSMAINI GUSMAINI, GUSMAINI HAJRIAL ASWIDINNOOR Hajrial Aswidinoor Halimatus Syahdia Hasibuan Hamim Hamim Hanedi Darmasetiawan HANNA ARTUTI EKAMAWANTI Hapsoh Hidayah, Agus Nur I Ketut Suada IBNUL QAYIM Ida Hanarida Soemantri Ika Mariska Ika Mariska Ika Mariska Ilona Noyaa, Alce Imam Widodo Inez Hortense Slamet Loedin Inez Hortense Slamet Loedin Inez Hortense Slamet-Loedin INEZ HORTENSE SLAMET-LOEDIN Iskandar Lubis Joko Prasetiyono Joko Prasetiyono Juang Gema Kartika K. Idris Karlin Agustina Kartika Ning Tyas Ketty Suketi KINYA AKASHI KINYA AKASHI Kisman Kisman Komaruddin Idris Komaruddin Idris La Muhuria Laela Sari Laela Sari laela Sari, laela Lukman Chakim Luluk Setyaningsih Lutfy Ditya Cahyanti, Lutfy Ditya M A Chozin M. A. Chozin Maemonah, Maemonah Mahpuzah, Marfiatun Mariska, Ika Masdiar Bustamam Masdiar Bustamam Masumi Moritsugu Matra, Deden Derajat Maya Melati Mayang Sari Meliala, Merry Gloria Moh. Yani Momongan, Jorex Daniel Muhamad Syukur Muhammad Arif Yudiarto Muhammad Jusuf Munarti Munif Ghulamahdi Muzuni, Muzuni Nani Heryani Nasution, Annisa Andrini Ni Made Armini Wiendi NURLIANI BERMAWIE Nurliani Bermawie Nurliani Bermawie Nurul Khumaida Osmat Azzam Jefferson Osmat Azzam Jefferson, Osmat Azzam OuwerkerV, Pieter BF Pieter B.F. Ouwerkerk Pieter BF OuwerkerV Putri Andini Mandasari RADITE TISTAMA Ragapadmi Purnamaningsih RAGAPADMI PURNAMANINGSIH Ratna Yuniati Rini, Erin Puspita Roedhy Poerwanto Rossa Yunita Rossa Yunita Rossa Yunita Rosyad, Astryani S. Suharsono Saepudin, Adam Sandi, Yusri Iryas Sandra Arifin Aziz Saragih, Ery Leonardo Sari, Laela Sarsidi Sastrosumarjo SATRIYAS ILYAS Satya Nugroho Sinaga, Falencia Sinaga, Parlin H. Sintho Wahyuning Ardie Siti Marwiyah Slamet Loedin, Inez Hortense Sobir Sobir SOEKISMAN TJITROSEMITO Soeranto Human Sri Mulatsih SRI RAHAYU Sri Setyati Harjadi Sri Wilarso Budi Sudarmonowati, Enny Sudirman Yahya Sudirman Yahya Sugianta Sugianta Sugianta, Sugianta SUGIONO MOELJOPAWIRO Sugiono Moeljopawiro Suharsono . Suharsono Suharsono Suharsono Suharsono Suhartanto, Muhammad Rahmad Sumiati, dan Sungkono Sungkono Supijatno Surjono Hadi Sutjahjo Suwarno Suwarno Suwarno Suwarno Suwarto Suwarto Syamsidah Rahmawati Syamsidah Rahmawati, Syamsidah T. Kawasaki T. M. H. Oelim Tanari, Yulinda Tintin Suhartini Titin Handayani Toshio Kawasaki Toyip Toyip TRI KOESOEMANINGTYAS Tri Lestari Trikoesoemaningtyas Utami, Nita Nur UTUT WIDYASTUTI Utut Widyastuti UTUT WIDYASTUTI Utut Widyastuti Suharsono Wage Ratna Rohaeni Wahju Q. Mugnisjah Wahju Qamara Mugnisjah YADI SETIADI Yadi Setiadi Yadi Setiadi Yadi Setiadi Yonny Koesmaryono Yopie Moelyohadi Yuli Sulistyowati YULIN LESTARI Yuniati, Ratna Yunita, Rossa