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All Journal CHEMISTRY PROGRESS MEDIA MEDIKA INDONESIANA Zuriat Jurnal Natur Indonesia Jurnal Agrista Microbiology Indonesia Journal of Tropical Soils Plumula : Berkala Ilmiah Agroteknologi Jurnal Sain Peternakan Indonesia Jurnal Peternakan Indonesia Jurnal Riset Kimia Jurnal Agroekoteknologi Indonesian Journal of Biotechnology BERITA BIOLOGI The Indonesian Biomedical Journal JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Universa Medicina Livestock and Animal Research JURNAL GALUNG TROPIKA As-syifaa Jurnal Farmasi Jurnal Penelitian Pendidikan IPA (JPPIPA) Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) JURNAL AGRONIDA Jurnal Hilirisasi IPTEKS (JHI) Buletin Ilmiah Nagari Membangun JERAMI Indonesian Journal of Crop Science Eksakta : Berkala Ilmiah Bidang MIPA Majalah Kedokteran Andalas Jurnal Agronomi Tanaman Tropika (JUATIKA) International Journal of Agricultural Sciences Jurnal Riset Teknologi Industri Unri Conference Series: Community Engagement Indian Journal of Forensic Medicine & Toxicology Narra J Pancaran Pendidikan Rafflesia Journal of Natural and Applied Sciences (RJNAS) Bioma : Jurnal Ilmiah Biologi
Jamsari Jamsari
Universitas Andalas
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Preparasi DNA Spesies Colletotrichum sp. dan Spesifitas Sistem Fingerprinting RAPD Jamsari, Jamsari
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.2 KB) | DOI: 10.31258/jnat.11.1.31-39

Abstract

DNA preparation from C. capsici dan C. gleosporides is important for PCR based analysis of anthracnose causingpathogens. On the other hand, RAPD as one of DNA-based fingerprinting is hindered by its instability and its nonspecifity problems. For that reason, converting RAPD fragments into other DNA based systems is an alternative toincrease its analysis reliability. The study showed that mycelia grown in 2-days liquid culture is appropriate materialfor DNA preparation especially when combined with Shagai-Maroof protocol as well as Promega Genomic DNAIsolation Kit. The study indicated also that some isolated RAPD-fragments showed its instability character. Thiswas proved by the occurrence of multi different length of fragments after re-amplification of some single RAPDspecific fragments.
BARKOD DNA PADA TANAMAN GAMBIR (Uncaria gambir(Hunter) Roxb.) BERDASARKAN GEN matK DAN rbcL Wardi, Epi Supri; Jamsari, Jamsari; Irwandi, Irwandi; Sartika, Diza; Ningsih, Azma Risilvia
Jurnal Ilmiah As-Syifaa Vol 12, No 1 (2020): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (435.864 KB) | DOI: 10.33096/jifa.v12i1.587

Abstract

Gambir (Uncaria gambir (Hunter) Roxb.) is a superior commodity of West Sumatra that has many benefits and has been used as a drug. Plant identification carried out morphologically has many weaknesses, with the development of electronic and genetic technology now a new method of species identification has been developed. plants and animals, namely DNA barcoding technology that uses standard short pieces of DNA Species identification methods have been agreed to using standard DNA barcodes are rbcL genes and matK genes. The purpose of this study is to find out DNA Barkoding candidates between matK and rbcL which can identify gambier (Uncaria gambir (Hunter) Roxb.) Which is good. Polymerase Chain Reaction (PCR) technique is used to amplify rbcL and matK gene sequences through universal primers. The DNA sequences of the MATC sequence in the GR sample (Riau Gambir) produced a similarity rate of 98.79% with Nauclea diderrichii, for the rbcL sequence GC (Cubadak Gambir) samples produced a similarity rate of 99.81% with Uncaria macrophylla, and GR (Gambir Riau) samples produced the similarity rate was 96.84% with Uncaria macrophylla based on analysis with BOLD Systems. This similarity indicates the low variation in intraspecific genetics for the identification or confirmation of gambir species
UJI DAYA ADAPTASI BEBERAPA VARIETAS BAWANG PUTIH (Allium sativum L.) DI KABUPATEN PESISIR SELATAN Obel, Obel; Resigia, Elara; Jamsari, Jamsari
Jurnal Agroekoteknologi Vol 12, No 2 (2020)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v12i2.10848

Abstract

Garlic (Allium sativum L.) is one of the important commodities in the horticultural sub-sector which is one of the basic needs of the community that must be fulfilled. However, garlic production in Indonesia has not been able to meet the demands of society's needs. For this reason, an additional area of land for garlic development is aimed at the optimal suitability of environmental physical factors. The use of superior varieties is one of the technologies that play a role in increasing the productivity of garlic. This research was conducted in May - August 2020 in Tarusan and Muaro Aie Bayang Utara, Pesisir Selatan Regency, West Sumatra Province with a randomized block design using Lumbu Hijau, Lumbu Kuning and local varieties of Bukik Sileh Solok as treatment. The results showed that the varieties of Lumbu Kuning and Lumbu Hijau were able to adapt well in Muaro Aie with an altitude of 322 masl compared to Tarusan with an altitude of 4 masl. Both varieties gave a fairly good growth response and yield, although only half of the description of these varieties.
Significant Association of Adam 33 Polymorphism with COPD in Javanese Population of Indonesia Retno Ariza Soeprihatini Soemarwoto; Jamsari; Yanwirasti; Andika Chandra Putra; Syazili Mustofa; Wawan Abdullah Setiawan; Ifan Aulia Candra
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.17045

Abstract

Background: Chronic Obstructive Pulmonary Disease (COPD) is one of World health cases that is commonlyknown, which is triggered by the combination of environmental factors especially cigarette smoking andgenetic factors. The association between A disintegrin and metalloprotease 33 (ADAM33) polymorphismsand COPD has been investigated and reported by other researchers. Objective: The main aim of this study isto identify the association between single nucleotide polymorphisms (SNPs) in ADAM 33 gene with COPDin the Javanese population in Lampung, Indonesia. Methods: A randomized cross-sectional study was usedin this research. PCR-Sequencing method was involved to analyze the polymorphic for three SNPs (T1, T2,and Q-1) of the ADAM33 gene. Statistical analysis data was performed in descriptive and comparative aswell as it was measured by parametric/non-parametric tests. Results: The results showed that the T2 GG,and T1AG genotypes in COPD group were significantly more frequent rather than in control group (p <0.05). In case of allele, it was found that the T1G and T2G was higher in COPD group rather than in thecontrol group (p = 0.440 and 0.131, respectively). Conclusion: The results clearly conclude that there wassignificant association between T1 and T2 polymorphisms of ADAM33 gene and COPD in the Javanesepopulation of Lampung, Indonesia.
Induksi Kalus dengan BAP (Benzylaminopurin) dan IAA (Indoleacetic acid) pada Tanaman Cabai (Capsicum annuum L.) Lokal Genotipe Lotanbar Sumatera Barat Sihotang, Saipul; Renfiyeni, Renfiyeni; Suliansyah, Irfan; Jamsari, Jamsari
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol 3, No 2 (2019): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, Juni
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agr.v3i2.2547

Abstract

This study was aimed to obtain the method in the study of callus induction of in vitro.  The research activities were carried out at Laboratory of Biotechnology, Faculty of Agrotechnology, Andalas University. This research used decriptive and experimental methods. One-way ANOVA analysis followed by Duncan’s test was used to determine significant differences (P ≤ 0.05). All statistical analysis were performed using the SPSS Ver. 23 statistical software package. The results showed that medium of callus induction is 4,0 mg/L BAP + 0,5 mg/L IAA with formation of callus (days), and average of callus formed, namely 4,33 days,  and 9,67 callus.
Pertumbuhan dan Hasil 6 Varietas Bunga Matahari (Helianthus annus L.) Pada Lahan Bukaan Baru di Dataran Tinggi Alahan Panjang Ramadhan, Nugraha; Martinsyah, Rachmad Hersi; Jamsari, Jamsari
JURNAL GALUNG TROPIKA Vol 11, No 1 (2022)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v11i1.870

Abstract

Bunga matahari merupakan komoditi yang memiliki potensi untuk dikembangkan sebagai bahan baku penghasil minyak nabati karena mengandung vitamin E yang cukup tinggi. Tanaman ini memiliki daya adaptasi yang luas dan membutuhkan daerah dengan sinar matahari penuh. Walau demikian, hanya varietas tertentu yang mampu untuk tumbuh dan berkembang optimal diberbagai agroekosistem. Sehinga diperlukan suatu informasi mengenai kesesuaian varietas dengan kondisi spesifik suatu lokasi. Penelitian ini bertujuan untuk memperoleh informasi tentang varietas yang adaptif pada lahan bukaan baru di dataran tinggi Alahan Panjang, Kabupaten Solok, Sumatera Barat (1.620 mdpl) berdasarkan pertumbuhan dan hasil yang dicapai. Percobaan dirancang menggunakan Rancangan Acak Kelompok (RAK) dengan 3 kelompok dan 5 taraf perlakuan, yaitu varietas bunga matahari. Varietas meiputi IPB BM 1, Ring of fire, Russian Mammoth, Hoppy Black Dye, Black Russian, dan Kanigara. Varietas Russian Mammoth, Ring of Fire dan Hopi Black Dye memiliki pertumbuhan yang lebih baik apabila dilihat dari keadaptifannya di lokasi penelitian. Sedangkan berdasarkan bobot biji/tanaman, persentase biji bernas, dan bobot 100 butir varietas Russian mammoth memiliki hasil yang terbaik.Sunflower is a commodity that has the potential to be developed as a raw material for producing vegetable oil because it contains high levels of vitamin E. This plant has wide adaptability and requires areas with full sun. However, only certain varieties are able to grow and develop optimally in various agroecosystems. So we need information regarding the suitability of varieties with specific conditions of a location. This study aims to obtain information about varieties that are adaptive to new openings in the highlands of Alahan Panjang, Solok Regency, West Sumatra (1,620 masl) based on growth and yields achieved. The experiment was designed using a Randomized Block Design (RAK) with 3 groups and 5 levels of treatment, namely sunflower varieties. The varieties include IPB BM 1, Ring of fire, Russian Mammoth, Hoppy Black Dye, Black Russian, and Kanigara. The varieties of Russian Mammoth, Ring of Fire, and Hopi Black Dye have better growth when viewed from their adaptability in the research location. Meanwhile, based on the weight of seeds/plant, the percentage of pithy seeds, and the weight of 100 grains the Russian mammoth variety had the best results.
Pertumbuhan dan Hasil Bunga Matahari Pada Lahan Tidur di Lahan Pantai Kabupaten Pesisir Selatan Obel, Obel; Ninda Rosadi, Firsta; Jamsari, Jamsari; Rahmad, Ali; Seswita, Seswita
JURNAL GALUNG TROPIKA Vol 11, No 1 (2022)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v11i1.866

Abstract

Lahan di kawasan pantai pemanfaatannya belum optimal untuk lahan pertanian karena dihadapkan pada beberapa masalah baik dari segi ekonomis maupun praktek budidaya. Perlu suatu gagasan dalam mengoptimalkan potensi lahan tersebut agar dapat lebih produktif dan memberi manfaat nyata pada masyarakat. Salah satu cara untuk memanfaatkan lahan pantai adalah dengan melakukan budidaya bunga matahari. Tujuan penelitian ini untuk mengetahui pertumbuhan dan hasil bunga matahari pada lahan tidur di kawasan pesisir. Penelitian disusun menggunakan Rancangan Acak Kelompok, dengan perlakuan 3 varietas dan 2 aksesi bunga matahari yang diulang sebanyak 3 kali. Hasil penelitian menunjukkan bunga matahari sangat berpotensi untuk dikembangkan pada  lahan tidur di daerah pantai Pesisir Selatan. Aksesi Ha1 menunjukkan pertumbuhan dan hasil yang lebih baik dibandingkan dengan aksesi Ha15 dan varietas Kanigara, Lokal Jumbo serta IPB BM1. Land in the coastal area has not been optimally utilized for agricultural land due to a number of issues, both economically and in terms of cultivation practices. An idea for maximizing the land's potential so that it can be more productive and deliver concrete benefits to the community is required. Sunflower cultivation is one technique to make use of coastal land. The goal of this study was to see how well sunflowers grew and yielded on unused land in coastal areas. The study used a randomized block design, with treatments of three sunflower kinds and two accessions repeated three times. The findings revealed that sunflowers have the potential to be grown on undeveloped land along the coast of the United States.
Adaptation Of Sunflowers To Salinity Stress On Coastal Land Silvia Permata sari; Obel Obel; Elara Resigia; Winda Purnama Sari Purnama Sari; Jamsari Jamsari; Ali Rahmad; Seswita Seswita
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol 4 No 1 (2022): Jurnal Agronomi Tanaman Tropika (JUATIKA)
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v4i1.1697

Abstract

Sunflower is one of the oil-producing plants that potentially can be developed as a food raw material, industrial, medicine, and cosmetics. Sunflower cultivation needs to be conducted in various lands in Indonesia; one of them is coastal regions. However, on one side, these lands were included on marginal land because the high level of salinity constrains it. This research aims to determine sunflower growth and thrive on salinity stress in the coastal land. This research applies a random group design with five treatments consisting of two accession and three sunflower varieties with three repetitions. The research result shows that the sunflower accession Ha1 growth and thrive on salinity stress in coastal land was quite significant compared to the accession Ha15 and Kanigara, Helina IPB, and BM1 IPB varieties. Generally, sunflower can adapt to stress salinity in coastal land.
STUDI AWAL EMPAT ISOLAT BAKTERI ANTAGONIS TERHADAP JAMUR Colletotrichum gloeosporioides Elly Syafriani; Femy Riwany; Rahmi Handayani; Rahmi Kamelia; Istino Ferita; Fatchiyah Fatchiyah; Jamsari Jamsari
Plumula : Berkala Ilmiah Agroteknologi Vol 6 No 2 (2018): Plumula : Berkala Ilmiah Agroteknologi
Publisher : UPN VETERAN JAWA TIMUR

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.985 KB) | DOI: 10.33005/plumula.v6i2.9

Abstract

Anthracnose disease on some important crops is caused by the Colletotrichum gloeosporioides. Because of this disease, many farmers were suffer a financial loss. The conventional way to solve this problem that taken by the farmers is synthetic fungicides application which has a negative impact on consumers and the environment. Another alternative to replace it is needed. Antagonistic microorganisms can be used to replace it then and to develop a biofungicide which is more safety for the consumers and the environment. Four isolates of antagonist bacteria to against C. gloeosporioides have been found, namely: UBCR_12, UBCR36, UBCF_01, and UBCF_13. They were being developed as the main material for the manufacture of biofungicide. As an initial step of it, some tests were conducted to identify and to obtain some information that related to the character of all the isolates. Gram staining and KOH test showed that UBCR_12, UBCR_36, and UBCF_01 were gram negative bacteria and UBCF_13 is gram positive bacteria. The four isolates were also showed rod morphological form. Oxidative-fermentative (OF) test showed that the four isolates are facultative anaerobic bacteria. The protease enzyme activity test showed that only 3 of 4 isolates can produced a protease enzyme with the highest clear zone index to the lowest, respectively are UBCR_12, UBCF_13, and UBCF_01. This information leads to choosing UBCR_12 for the molecular identification by the cloning 16S rRNA gene of it. The result showed that UBCR_12 is Serratia plymuthica.
Variabilitas Fenotipik dan Kekerabatan Genetik Spesies Gambir Liar Murdaningsih H. Karmana; Hamda Fauza; , Jamsari; Azmi Dhalimi; Ahmad Denian; Istino Ferita; , Nurainas
Zuriat Vol 20, No 2 (2009)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v20i2.6632

Abstract

Pada tanaman gambir, belum didapatkan informasi yang lengkap dan akurat tentang keberadaan plasma nutfah yang merupakan sumber materi genetik untuk pemuliaan. Informasi mengenai tingkat variabilitas genetik serta hubungan kekerabatan di antara populasi gambir liar sangat diperlukan oleh pemulia tanaman untuk mengidentifikasi calon tetua yang potensial. Untuk mengetahui variabilitas fenotipik dilakukan pengamatan terhadap karakter morfologi dan agronomi gambir liar serta diestimasi variabilitas genetiknya menggunakan analisis RAPD. Bahan tanaman yang digunakan untuk penentuan variabilitas genetik adalah tujuh spesies gambir liar yang merupakan sebagian dari ekplorasi dan yang memiliki sampel lebih dari 10 aksesi. Pengamatan dilakukan pada 111 aksesi terhadap 12 karakter fenotipik. Hubungan kekerabatan antar spesies diketahui melalui analisis klaster berdasarkan teknik RAPD terhadap 28 aksesi, tiga aksesi untuk setiap spesies yang jumlah aksesinya sama atau lebih dari tiga atau sesuai dengan jumlah aksesi yang ada pada setiapspesies tersebut. Hasil pengamatan terhadap karakter fenotipik spesies gambir liar pada beberapa lkasi di Sumatera Barat, menunjukkan variabilitas fenotipik yang luas pada beberapa karakter yang diamati. Analisis klaster berdasarkan karakter fenotipik antar spesies memperlihatkan antar spesies memiliki jarak taksonomi yang bervariasi. Hasil amplifikasi DNA dengan teknik RAPD memperlihatkan profil pita DNA yang berbeda di antara spesies gambir liar. Analisis kekerabatan berdasarkan profil pita DNA menunjukkan terdapat jarak genetik yang bervariasi di antara spesies gambir liar, yang berarti spesies gambir liar memiliki variabilitas genetik yang luas. Namun, tidak terdapat korelasi yang erat antara pengamatan data fenotipik dengan data genetik (profil pita DNA).
Co-Authors -, Urnemi -, Vovien . Dachryanus Abdi Dharma Achmad Noerkhaerin Putra Adrial Adrial, Adrial Afriwardi Afriwardi Agustian Agustian Ahmad Denian Ali Rahmad Ali Rahmat Aliya, Lisana Shidiqqin Alvarino Ananda, Abi Awfa Rahman Andani Eka Putra Andika Ade Saputra Arif Sardi Armaini - Arni Amir Arnim Arnim Asman Manaf Azhar, Minda Azmi Dhalimi Buana, Gherriandi R. Cuandra, Kevin N. Dachryanus, Dachryanus Delmi Sulastri DESSY NATALIA Djong Hon Tjong E. Y. Rahman E. Y. Rahman Efrida Efrida ekawati, fitri elara resigia Elly Syafriani Elmatris , Elmatris ELVI ZURIYANI Elvi Zuriyani Endang Purwati RN Endrinaldi , Endrinaldi Eryati Darwin Eryka Okta Juanty Eryka Okta Juanty FA Febria Fatchiyah Fatchiyah Febria, FA Femy Riwany Firjatullah, Muhammad F. Friska Eka Fitria Gumilar, Erry Gusmawartati Gusmawartati Hamda Fauza Hanifah, Wafiq Hardisman Hasbi Al Huda Hasibuan, Imron Martua Hazmi, Auliya A. Herviyanti Herviyanti Ida Parwati Ifan Aulia Candra Irawati Chaniago Irfan Suliansyah ISHAK Ishak Manti Ishak Manti ISHAK MANTI Istino Ferita Jeanne Venora Jumsu Trisno Karmia, Hudila Rifa Lidya Sari Utami Lily Syukriani Lipoeto NI Liza Aulia Yusfi Maharani, Annisa D. Maulana, Rafi Meisilva Erona Meisilva Erona S Minda Azhar Mirnawati Mirnawati ML Edy Parwanto Mochammad Imron Awalludin Muhamad Irsyad Murdaningsih H. Karmana N Nasir N Nurhidayat N Nurhidayat, N Nasril Nasir Nasril Nasir Nasrul Zubir Natalia, Dessy Nefilinda Nefilinda Nefilinda Nefilinda Nelson, Nelvianti Netty Suharti Ningsih, Azma Risilvia Noraziyah Abd Aziz Shamsudin Nur Afrainin Syah Nurainas Nurainas Nursyafi, Fauzan Syarif Obel Obel Obel, Obel Prayogi, Norbertus M. Putra, Andika Chandra Rachmad Hersi Martinsyah Rahmad, Ali Rahman, Awalil RK. Rahmi Handayani Rahmi Kamelia Ramadhan, Nugraha Renfiyeni Reni Koja Respati, Aditya Nugraha Retno Ariza Soeprihatini Soemarwoto Rhenly Danis Rika Despica Rika Despica Rosadi, Firsta Ninda Roza Yunita Rusfidra rusfidra Sahrul Utama Saipul Sihotang, Saipul Salma Salma Salma Salma Sanusi Ibrahim Sarbaini Sarbaini Sartika, Diza Selvia Dewi Pohan Seswita, Seswita Silvia Permata sari Slamet Rianto SRI HENDRASTUTI HIDAYAT Sumaryati Syukur Sumaryati Syukur Suswita, Rini Syamsurizal Syamsurizal Syazili Mustofa Tika Runifah Trimuri Habazar TRIMURTI HABAZAR Tristan, Christopher D. Urnemi - Urnemi Urnemi V. Maslami Vovien - Wahyudi, Dhyani P. Wardi, Epi Supri Wawan Abdullah Setiawan Winda Purnama Sari Winda Purnama Sari Y Heryandi Y. Marlida Y. S. Nur Yahono, Angela S. Yanwirasti Yanwirasti Yanwirasti Yenny, Satya W. Yuniar Lestari Yurnalis Yurnalis Yusrawati Yusrawati Yustini Alioes ZK Abdurahman Baizal Zulkarnain Chaidir