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Isolation of Cellulolytic Bacteria from Peat Soils as Decomposer of Oil Palm Empty Fruit Bunch . Gusmawartati; . Agustian; . Herviyanti; . Jamsari
JOURNAL OF TROPICAL SOILS Vol 22, No 1: January 2017
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2017.v22i1.47-53

Abstract

The aim of the research was to find out potential strainsof cellulolytic bacteria isolated from two tropical peat soils and to studythe potency of the isolated bacteria to decompose oil palm empty fruit bunch (EFB). The research was carried out in two stages: (1) isolation of cellulolytic bacteria from peat soils and (2) testing the potency of isolated bacteria to decompose oil palm EFB. The cellulolytic bacteria were isolated from two peat soils, i.e. a natural peat soil (forest) and a cultivated peat soil (has been used as agriculture land). Isolation of cellulolytic bacteria was conducted by preparing a series dilution of culture solutions using a streak plate method in a carboxymethyl cellulose(CMC) selective medium.Isolates that were able to form clear zones surrounding their bacterial colony were further tested to study the potency of the isolates to decompose cellulose in oil palm EFB. The cellulolytic activity of the selected isolates were further determined via production of reducing sugars in an oil palm EFB liquid medium using Nelson-Somogyi method. The results showed that there are six isolates of cellulolytic bacteria that have been identified in two tropical peat soils used in the current study. Two isolates are identified in a natural peat soil (forest) and four isolates are identified in a cultivated peat soil. The isolates collected are identified as Bacillus sp., Pseudomonassp. and Staphylococcus sp. Among the isolates, an isolate of GS II-1 produces the highest concentration of reducing sugars, namely 0.1012 unitmL-1or 101 ppm, indicating that the isolate of GS II-1 is highly potential to decompose oil palm EFB. Therefore, the isolate of GS II-1 can be used as a decomposer in the bio-conversion processes of oil palm EFB.Keywords: isolation, bacteria, cellulolytic, oil palm empty fruit bunch (EFB), peat soil
IDENTIFIKASI GEN PENYANDI PIREN DIOKSIGENASE PADA ISOLAT BAKTERI PENDEGRADASI PIREN FA Febria; Jamsari Jamsari; N Nasir; N Nurhidayat
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1946

Abstract

The pyrene dioxygenase coded by gene is an indicator of bacterial isolates capabilities in pyrene degradation. The encoded gene of pyrene dioxygenase can be amplified and detected in Pseudomonas sp. PyrA2 and Burkholdoriasp. PyrA4 isolates, using primary specific Diox which designed based on PhdF gene sequence, the coding gene of pyrene dioxygenase in Mycobacterium vanbalenii PYR-1. The sequence alignment of pyrene dioxygenase putative gene in both of bacterial isolates with the sequence of pyrene dioxygenase coding gene in M. vanbalenii PYR-1 shows the similarity percentage of 41 % and 42% with Pseudomonas sp. PyrA2 and Burkholdoria sp.PyRA4.
INDUKSI KETAHANAN TANAMAN JAHE TERHADAP PENYAKIT LAYU Ralstonia solanacearum RAS 4 MENGGUNAKAN FUNGI MIKORIZA ARBUSKULAR (FMA) INDIGENUS Netty Suharti; Trimurti Habazar; Nasril Nasir; . Dachryanus; . Jamsari
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 1 (2011): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.073 KB) | DOI: 10.23960/j.hptt.111102-111

Abstract

Bacterial wilt disease caused by Ralstonia solanacearum race 4 is a yield-limiting disease of ginger. A study to investigate the ability of indigenous Arbuscular Mycorrhizae Fungi (AMF) to reduce bacteria wilt disease has been done. This research was designed by using Randomized Block Design (RBD) at greenhouse experiments, using eight AMF isolates with six replication. As supporting data, population R. solanacearum race 4 after treatments was assesed. The results showed that aplication of indigenous AMF to ginger rhizome as seed treatment dicreased disease incidence, support ginger growth and production such as the seedling formation 150%, plant height 98.6%, the number of leaf 115,7%. As the result yield of ginger rhizome increased by 400 % and phyloplan 598,3%, respectively, compared to the control.
Karakterisasi Sumber Daya Genetik Itik Bayang Berdasarkan Marka Mikrosatelit Lokus CMO211 dan Lokus AY295 Rusfidra Rusfidra; Y. Heryandi; Jamsari Jamsari; E. Y. Rahman
Jurnal Sain Peternakan Indonesia Vol 9, No 1 (2014)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.9.1.19-29

Abstract

ABSTRAKItik Bayang merupakan plasma nutfah Sumatera Barat yang berperan penting sebagai penghasil daging dan telur. Marka mikrosatelit banyak digunakan sebagai penciri genetik karena keberadaannya berlimpah, bersifat ko-dominan dan polimorfik. Tujuan penelitian ini adalah untuk mengidentifikasi keragaman genetik itik Bayang berdasarkan marka mikrosatelit lokus CMO211 dan AY295. Material DNA yang digunakan adalah sampel darah dari 24 ekor itik Bayang di Kecamatan Bayang dan Kecamatan Lengayang Kabupaten Pesisir Selatan. Hasil isolasi DNA dari 24 sampel darah itik Bayang dapat dideteksi oleh gel elektroforesis. Lokus CMO211 menampilkan 2 alel, yakni alel A dan alel B dengan panjang alel 150–200 bp. Frekuensi kedua alel adalah alel A (87,5%) dan alel B (12,5%). Lokus AY295 menampilkan 7 alel, yaitu alel A (214 bp), alel B (245 bp), alel C (273 bp), alel D (300 bp), alel E (324 bp), alel F (359 bp) dan alel G (454 bp). Frekuensi alel berturut-turut adalah alel B (24%), alel A (16%), alel C (16%), alel D (16%), alel E (12%), alel F (12%) dan alel G (4%). Hasil amplifikasi menunjukkan lokus CMO211 dan AY295 bersifat polimorfik pada itik Bayang.Kata kunci : itik Bayang, mikrosatelit, lokus CMO211, lokus AY295
UJI DAYA ADAPTASI BEBERAPA VARIETAS BAWANG PUTIH (Allium sativum L.) DI KABUPATEN PESISIR SELATAN Obel Obel; Elara Resigia; Jamsari Jamsari
Jurnal Agroekoteknologi Vol 12, No 2 (2020)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v12i2.11494

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Garlic (Allium sativum L.) is one of the important commodities in the horticultural sub-sector which is one of the basic needs of the community that must be fulfilled. However, garlic production in Indonesia has not been able to meet the demands of society's needs. For this reason, an additional area of land for garlic development is aimed at the optimal suitability of environmental physical factors. The use of superior varieties is one of the technologies that play a role in increasing the productivity of garlic. This research was conducted in May - August 2020 in Tarusan and Muaro Aie Bayang Utara, Pesisir Selatan Regency, West Sumatra Province with a randomized block design using Lumbu Hijau, Lumbu Kuning and local varieties of Bukik Sileh Solok as treatment. The results showed that the varieties of Lumbu Kuning and Lumbu Hijau were able to adapt well in Muaro Aie with an altitude of 322 masl compared to Tarusan with an altitude of 4 masl. Both varieties gave a fairly good growth response and yield, although only half of the description of these varieties. 
Transcription factor 7-like 2 as type-2 diabetes mellitus diagnostic marker in ethnic Minangkabau Syamsurizal Syamsurizal; Yanwirasti Yanwirasti; Asman Manaf; Jamsari Jamsari; ML Edy Parwanto; Arif Sardi
Universa Medicina Vol. 33 No. 3 (2014)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2014.v33.205-212

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BACKGROUNDThe prevalence of type-2 diabetes mellitus (T2DM) has been increasing rapidly in developed and developing countries. Many new loci associated with T2DM have been uncovered by genetic and genome-wide association studies, eg. the transcription factor 7-like 2 (TCF7L2) gene variant rs7895340 has been associated with type-2 diabetes mellitus. The aim of this research was to find an association between polymorphisms of TCF7L2 gene variant rs7895340 with T2DM in ethnic Minangkabau.METHODSThis was a case-control study using a consecutive sampling technique among ethnic Minangkabau patients who came for treatment to the Metabolic Endocrinology Polyclinic at Dr. M. Jamil Hospital. Analysis was performed on 132 subjects (66 T2DM patients and 66 controls). DNA was isolated from venous blood samples and amplified using the amplification refractory mutation system - polymerase chain reaction (ARMS-PCR) method to detect single nucleotide polymorphisms (SNPs) of TCF7L2 gene variant rs7895340. To determine the significance of association of SNPs of rs7895340 with T2DM in ethnic Minangkabau, data analysis was performed using chi-square test.RESULTSThe ARMS - PCR for TCF7L2 gene variant rs7895340 found polymorphisms in 28 (42.42%) subjects with type 2 diabetes mellitus and in 15 (22.72%) non-diabetic subjects.There was a significant association between rs7895340 SNPs with the probability of T2DM in ethnic Minangkabau (OR=2.505, p=0.026). CONCLUSIONSSingle nucleotide polymorphism rs7895340 of TCF7L2 gene can be used as genetic marker of type-2 diabetes mellitus in ethnic Minangkabau.
PEMANFAATAN PLASTIK BEKAS SEBAGAI MEDIA TANAM RAMAH LINGKUNGAN DI KELURAHAN BUNGO PASANG KOTA PADANG Nefilinda Nefilinda; Jamsari Jamsari; Elvi Zuriyani; Rika Despica; Eryka Okta Juanty; Salma Salma
BULETIN ILMIAH NAGARI MEMBANGUN Vol 3 No 3 (2020)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bina.v3i3.242

Abstract

Bungo Pasang Village is one of the areas in Padang City. This area has densely populated settlements and houses with limited land. These settlements produce waste in the form of abundant organic or inorganic waste, which will impact environmental pollution. The activity objectives are to increase public knowledge and understanding of using used plastic bottles as a medium for planting flowers and environmentally friendly living pharmacies. Methods of activity: 1). lecture, 2). demonstration/training of using used plastic bottles as planting media. This activity's target audience is women in the Salingka Bungo Permai 1 Complex, Bungoasang Village, Koto Tangah District, Padang City, with about 30 members. Participants were also taught practice: punching plastic bottles, tying plastic bottles, mixing soil and fertilizer, planting flower plants in plastic bottles, and arranging plants vertically. The results of the activity obtained can increase public knowledge and awareness about using used plastic bottles as a planting medium. Furthermore, the community understands using used plastic bottles as an environmentally friendly planting medium in a little yard to the application stage of using used plastic bottles as an environmentally friendly planting medium. It is hoped that this activity can be carried out by the community in a sustainable manner to benefit people who have narrow yardlands. Furthermore, better management is needed to manage inorganic waste, such as using used bottles as planting media.
Modern Plant Breeding For Sustainable Agriculture: A Review Selvia Dewi Pohan; Noraziyah Abd Aziz Shamsudin; Jamsari Jamsari; Tika Runifah
JERAMI : Indonesian Journal of Crop Science Vol 4 No 1 (2021): JIJCS
Publisher : Department of Crop Science, Faculty of Agriculture, Andalas University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jijcs.4.1.9-16.2021

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More foods are required to fulfill human need. The limitation of the farming area is leading to intensification system in crop cultivation, so the use of chemical properties and environmental destruction cannot be denied. However, this industrial practice in the crop system has caused many adverse effects on ecological balance and human health. Many severe impacts of industrial agriculture practices have been reported, including soil erosion, groundwater contamination, loss of productivity, depletion of fossil resources, air pollution, new threats to human health and safety. Many experts agreed that sustainable agriculture seems to be an effective way to solve these issues. Modern plant breeding is now a popular approach to provide superior crop cultivars that can minimize adverse effects of agriculture practices because humans can recognize and manipulated genes through this method. Recently, the application of molecular markers in plant breeding selection has been brought enthusiasm for plant breeders. Marker-assisted selection (MAS) has been evidenced to be a beneficial technique in plant breeding. Through this approach, the selection process of parents and progenies in the breeding process could be more efficient. Plant breeding is influential in crop production achievement because this effort is connected with the adaptability and stability of the varieties in many different environmental circumstances. Plant breeding also focuses on agriculture sustainability due to its effort to produces durable disease resistance, abiotic stress tolerance, nutrients, and water-use efficiency.
Effectivity of Arbuscular Mycorrhyza Fungi (AMF) and Nitrogen Fertilizer on The Growth and Yield of Sunflower (Helianthus annus L.) Firsta Ninda Rosadi; Roza Yunita; Jamsari Jamsari
JERAMI : Indonesian Journal of Crop Science Vol 4 No 1 (2021): JIJCS
Publisher : Department of Crop Science, Faculty of Agriculture, Andalas University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jijcs.4.1.29-32.2021

Abstract

One of the main products of sunflower is the seed which is a producer of vegetable oil that is needed in the pharmaceutical industry and the health sector. The price of sunflower seed oil is currently very high for the market but sunflower production in Indonesia is quite low. This potential can be used as the basis for the development of sunflower cultivation in Indonesia. One of the efforts to increase the content of sunflower seed oil is to improve proper cultivation techniques and modifications in cultivation techniques. One of the cultural techniques in increasing production is to get the right dose of nitrogen (N) fertilization. The addition of Arbuscular Mycorrhiza Fungi (AMF) is expected to increase the absorption of N nutrients in the soil, thereby increasing the growth and yield of sunflowers. This study aimed 1) effectivity of AMF and nitrogen fertilizer application with various doses on growth and yield of sunflower, 2) to obtain the right dose of N fertilizer on growth and yield of sunflower The study used a Split-plot Design with 3 replications. The first factor as the main plot is AMF i.e. F0 = without AMF, F1 = with AMF 5 g hole-1. The second factor as a sub-plot is the dose of urea fertilizer with 4 levels i.e. N1 = 50 kg N Ha-1, N2 = 100 kg N Ha-1, N3 = 150 kg N Ha-1, N4 = 200 kg N Ha-1. Observations data were evaluated by analysis of variance if the obtained F count was greater than F table 5% followed by a further test of DNMRT at the 5% significant level. Result showed 1) the effectivity of AMF and nitrogen fertilization on sunflowers was able to increase the number of leaves, accelerate the initiation of flowering, increase the diameter of sunflowers, total seeds per plant, the weight of 100 seeds, and seed production per plant, 2) Economically, the application of nitrogen fertilizer with a dose of 150 kg N Ha-1 is more appropriate in increasing the growth and production of sunflowers
Infeksi Ganda Pepper Yellow Leaf Curl Virus dan Chilli Veinal Mottle Virus dalam Menimbulkan Penyakit Daun Kuning Keriting Cabai Jumsu Trisno; Jamsari Jamsari; Sri Hendrastuti Hidayat
Jurnal Proteksi Tanaman Vol 5 No 2 (2021): December 2021
Publisher : Plant Protection Departement, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.5.2.77-88.2021

Abstract

Yellow leaf curl disease is the primary disease in chili plants with up to 100% losses. Detection from the field showed a mixed infection between Geminivirus or Pepper Yellow Leaf Curl Virus (PYLCV) and several viruses, including Chilli Veinal Mottle Virus (ChiVMV) and Cucumber mosaic Virus (CMV). Therefore, this study aimed to determine the role of PYLCV and ChiVMV dual infection in influencing the development of chili yellow leaf curl disease. This study used a completely randomized design (CRD) with six treatments and ten replications. The treatments used were multiple Geminiviruses and ChiVMV infections simultaneously, before ChiVMV co-infection, after Geminivirus infection, single infection and whithout infection viruses. The results showed that the presence of ChiVMV infection before, after, and concurrently with Geminiviruses infection could increase the yellow leaf curl disease intensity and reduce plant height and wet weight growth. The mean scale value of ChiVMV infection before, after, and concurrently with Geminiviruses infection were 3.38, 3.90 and 3.58 compared to single Geminiviruses infection (scale of 3.20) and single ChiVMV (scale of 1.33). The interaction analysis of ChiVMV and geminiviruses infections based on the percentage reduction in plant growth height and wet weight showed additive interaction. ChiVMV co-infection further exacerbated the incidence of yellow leaf curl diseases in chilies.
Co-Authors -, Urnemi -, Vovien . Dachryanus Abdi Dharma Achmad Noerkhaerin Putra Adrial Adrial, Adrial Afriwardi Afriwardi Agustian Agustian Ahmad Denian Ali Rahmad Ali Rahmat Aliya, Lisana Shidiqqin Alvarino Ananda, Abi Awfa Rahman Andani Eka Putra Andika Ade Saputra Arif Sardi Armaini - Arni Amir Arnim Arnim Asman Manaf Azhar, Minda Azmi Dhalimi Buana, Gherriandi R. Cuandra, Kevin N. Dachryanus, Dachryanus Delmi Sulastri DESSY NATALIA Djong Hon Tjong E. Y. Rahman E. Y. Rahman Efrida Efrida ekawati, fitri elara resigia Elly Syafriani Elmatris , Elmatris ELVI ZURIYANI Elvi Zuriyani Endang Purwati RN Endrinaldi , Endrinaldi Eryati Darwin Eryka Okta Juanty Eryka Okta Juanty FA Febria Fatchiyah Fatchiyah Febria, FA Femy Riwany Firjatullah, Muhammad F. Friska Eka Fitria Gumilar, Erry Gusmawartati Gusmawartati Hamda Fauza Hanifah, Wafiq Hardisman Hasbi Al Huda Hasibuan, Imron Martua Hazmi, Auliya A. Herviyanti Herviyanti Ida Parwati Ifan Aulia Candra Irawati Chaniago Irfan Suliansyah ISHAK ISHAK MANTI Ishak Manti Ishak Manti Istino Ferita Jeanne Venora Jumsu Trisno Karmia, Hudila Rifa Lidya Sari Utami Lily Syukriani Lipoeto NI Liza Aulia Yusfi Maharani, Annisa D. Maulana, Rafi Meisilva Erona Meisilva Erona S Minda Azhar Mirnawati Mirnawati ML Edy Parwanto Mochammad Imron Awalludin Muhamad Irsyad Murdaningsih H. Karmana N Nasir N Nurhidayat N Nurhidayat, N Nasril Nasir Nasril Nasir Nasrul Zubir Natalia, Dessy Nefilinda Nefilinda Nefilinda Nefilinda Nelson, Nelvianti Netty Suharti Ningsih, Azma Risilvia Noraziyah Abd Aziz Shamsudin Nur Afrainin Syah Nurainas Nurainas Nursyafi, Fauzan Syarif Obel Obel Obel, Obel Prayogi, Norbertus M. Putra, Andika Chandra Rachmad Hersi Martinsyah Rahmad, Ali Rahman, Awalil RK. Rahmi Handayani Rahmi Kamelia Ramadhan, Nugraha Renfiyeni Reni Koja Respati, Aditya Nugraha Retno Ariza Soeprihatini Soemarwoto Rhenly Danis Rika Despica Rika Despica Rosadi, Firsta Ninda Roza Yunita Rusfidra rusfidra Sahrul Utama Saipul Sihotang, Saipul Salma Salma Salma Salma Sanusi Ibrahim Sarbaini Sarbaini Sartika, Diza Selvia Dewi Pohan Seswita, Seswita Silvia Permata sari Slamet Rianto SRI HENDRASTUTI HIDAYAT Sumaryati Syukur Sumaryati Syukur Suswita, Rini Syamsurizal Syamsurizal Syazili Mustofa Tika Runifah Trimuri Habazar TRIMURTI HABAZAR Tristan, Christopher D. Urnemi - Urnemi Urnemi V. Maslami Vovien - Wahyudi, Dhyani P. Wardi, Epi Supri Wawan Abdullah Setiawan Winda Purnama Sari Winda Purnama Sari Y Heryandi Y. Marlida Y. S. Nur Yahono, Angela S. Yanwirasti Yanwirasti Yanwirasti Yenny, Satya W. Yuniar Lestari Yurnalis Yurnalis Yusrawati Yusrawati Yustini Alioes ZK Abdurahman Baizal Zulkarnain Chaidir