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Dual Infection of Pepper Yellow Leaf Curl Virus and Chilli Veinal Mottle Virus in Causing the Yellow leaf Curl Disease on Chili Trisno, Jumsu; Jamsari, Jamsari; Hidayat, Sri Hendrastuti
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 5 No. 2 (2021): December 2021
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.5.2.77-88.2021

Abstract

Yellow leaf curl disease is the primary disease in chili plants with up to 100% losses. Detection from the field showed a mixed infection between Geminivirus or Pepper Yellow Leaf Curl Virus (PYLCV) and several viruses, including Chilli Veinal Mottle Virus (ChiVMV) and Cucumber mosaic Virus (CMV). Therefore, this study aimed to determine the role of PYLCV and ChiVMV dual infection in influencing the development of chili yellow leaf curl disease. This study used a completely randomized design (CRD) with six treatments and ten replications. The treatments used were multiple Geminiviruses and ChiVMV infections simultaneously, before ChiVMV co-infection, after Geminivirus infection, single infection and whithout infection viruses. The results showed that the presence of ChiVMV infection before, after, and concurrently with Geminiviruses infection could increase the yellow leaf curl disease intensity and reduce plant height and wet weight growth. The mean scale value of ChiVMV infection before, after, and concurrently with Geminiviruses infection were 3.38, 3.90 and 3.58 compared to single Geminiviruses infection (scale of 3.20) and single ChiVMV (scale of 1.33). The interaction analysis of ChiVMV and geminiviruses infections based on the percentage reduction in plant growth height and wet weight showed additive interaction. ChiVMV co-infection further exacerbated the incidence of yellow leaf curl diseases in chilies.
The Effect of Pesticide Exposure on Women Farmers of Reproductive Age on Growth and Development Disorders of Children Born: A Systematic Literature Review Fitria, Friska Eka; Syah, Nur Afrainin; Jamsari, Jamsari; Lestari, Yuniar
Jurnal Penelitian Pendidikan IPA Vol 9 No 9 (2023): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i9.5042

Abstract

The large number of children suffering from ADHD and autism today is a result of the increasing use of pesticides in agriculture. This applies to women farmers of childbearing age who work in agricultural settings or women whose work is related to the use of pesticides. Pesticides are a mixture of several chemicals used to control pests and protect plants. With the use of pesticides, yields obtained increased significantly. This study aims to examine the effect of exposure to pesticides on female farmers of childbearing age on growth and development disorders of children born. The review was conducted according to state-of-the-art methods using selected reporting item guidelines for reviews and meta-analyses (PRISMA). We reviewed the literature from several publications and analyzed the effect of exposure to pesticides on female farmers of childbearing age on developmental disorders of the children they were born with. The results of this study are the effect of exposure to pesticides on female farmers of childbearing age on birth defects. The use of pesticides causes pesticide poisoning. Accumulation of pesticides at a certain threshold has a negative impact on the body, namely brain disorders, tumors, cancer, even in pregnant women can cause birth defects. Apart from adults, pesticides also have a negative impact on children
Physicochemical Characteristics of Gum Arabic From Acacia mearnsii Grown in Alahan Panjang, West Sumatera Syukriani, Lily; Yunita, Roza; Rosadi, Firsta Ninda; Jamsari, Jamsari
AGROSCRIPT: Journal of Applied Agricultural Sciences Vol 6 No 2 (2024): December
Publisher : Universitas Perjuangan Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/agroscript.v6i2.2049

Abstract

Acacia gum produced from Acacia mearnsii is a natural material with great potential for various industrial applications due to its unique physicochemical properties. This study aimed to characterize gum acacia from Alahan Panjang, West Sumatra. Characterizatio was done using Fourier Transform Infrared Spectroscopy (FTIR), X-ray fluorescence (XRF), and X-ray diffraction (XRD) techniques. FTIR results revealed the presence of hydroxyl (-OH), carbonyl (-C=O), and glycosidic (C-O-C) groups, indicating the complex polysaccharide structure of the gum. XRF analysis showed the dominance of calcium oxide (CaO) with a concentration of 81.652%, followed by potassium oxide (K₂O) at 10.052% and phosphate (P₂O₅) at 6.341%. Meanwhile, the XRD diffraction pattern identified a near amorphous diffraction with some weak crystalline peaks. This combination of results suggests that gum acacia has chemical and physical properties that support its use as an emulsifier, thickener, and filler in various industries, such as food, pharmaceutical, and cosmetics.
Enhanced expression of L-asparaginase II by fusion of pSSPM3 synthetic promoter into pET-28a+ expression vector for alternative targeted therapy of acute lymphoblastic leukaemia Aliya, Lisana Shiddiqin; Julizar, Julizar; Rasyid, Roslaili; Pertiwi, Dian; Syukriani, Lily; Nursyafi, Fauzan Syarif; Saibi, Ihsan R. A.; Hasibuan, Imron Martua; Febiona, Keysha Putri; Jamsari, Jamsari
Bioma : Berkala Ilmiah Biologi Volume 27 Issue 2 Year 2025
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2025.72304

Abstract

The use of commercial L-asparaginase II from E. coli and Erwinia chrysanthemi for acute lymphoblastic leukemia (ALL) therapy causes adverse effects (allergic reactions, neurotoxicity), necessitating safer alternatives. Serratia plymuthica UBCF_13, harboring the ansB gene, offers a promising source. This study constructed the recombinant plasmid pET-28a+:pSSPM3:ansB to enhance L-asparaginase II expression in E. coli using the synthetic promoter pSSPM3. Molecular verification confirmed successful steps: promoter fusion (674 bp band), ansB digestion (1,057 bp), gene insertion (1,682 bp), and BL21 transformation. Critically, enzyme activity assays revealed that pSSPM3 did not enhance expression in BL21 (0.519 U/mL), showing significantly lower activity (p<0.05) than native controls (0.621 U/mL) and DH10B transformants (0.636 U/mL). While the functional plasmid establishes a platform for novel enzyme production, the unexpected activity reduction in BL21 and higher yield in DH10B highlight host-promoter compatibility challenges. Further optimization of expression systems, purification protocols, and preclinical validation (cytotoxicity, allergenicity) are essential to advance this recombinant enzyme toward therapeutic and scalable industrial applications for ALL in resource-limited settings.
Efektifivitas Intervensi Perubahan Perilaku Petani dalam Penggunaan Pestisida Berbasis Pendekatan Transtheoretical Model Friska Eka Fitria; Nur Afrainin Syah; Jamsari Jamsari; Yuniar Lestari
Jurnal Ners Vol. 10 No. 1 (2026): JANUARI 2026
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v10i1.52921

Abstract

Penggunaan pestisida yang tidak aman dan sehat masih menjadi persoalan serius di sektor pertanian dan meningkatkan risiko kesehatan petani. Penelitian ini bertujuan mengevaluasi efektivitas intervensi berbasis Transtheoretical Model (TTM) dalam mendorong perubahan perilaku penggunaan pestisida yang aman dan sehat pada petani hortikultura di Kabupaten Solok. Penelitian menggunakan desain one group pretest–posttest dengan 36 responden yang dipilih menggunakan quota sampling. Instrumen berupa kuesioner TTM yang memetakan lima tahap perilaku: pra-kontemplasi, kontemplasi, persiapan, tindakan, dan pemeliharaan. Intervensi diberikan satu kali melalui penyuluhan menggunakan media berupa video edukasi, diskusi kelompok dan konseling individu, dan pelatihan penggunaan APD dan praktik dosis serta pencampuran pestisida yang tepat. Hasil menunjukkan pergeseran signifikan tahap perilaku, dengan mayoritas petani bergerak dari kontemplasi ke persiapan dan tindakan. Uji Wilcoxon menghasilkan p=0,000 (p≤0,05), menandakan adanya perbedaan bermakna antara pretest dan posttest. Sebanyak 72,2% petani mengalami perubahan perilaku ke arah positif. Temuan ini menegaskan bahwa intervensi yang disesuaikan dengan tahapan kesiapan individu lebih efektif dalam mendorong perubahan perilaku, serta berpotensi menjadi dasar penyusunan strategi peningkatan kesehatan kerja petani yang berkelanjutan.
Co-Authors -, Urnemi -, Vovien . Dachryanus Abdi Dharma Achmad Noerkhaerin Putra Adrial Adrial, Adrial Agustian Agustian Ahmad Denian Ali Rahmad Ali Rahmat Aliya, Lisana Shiddiqin Aliya, Lisana Shidiqqin Alvarino Alvarino Alvarino Ananda, Abi Awfa Rahman Andani Eka Putra Andika Ade Saputra Arif Sardi Armaini - Arnim Arnim Asman Manaf Azhar, Minda Azmi Dhalimi Buana, Gherriandi R. Cuandra, Kevin N. Dachryanus, Dachryanus Delmi Sulastri DESSY NATALIA Dian Pertiwi Djong Hon Tjong E. Y. Rahman E. Y. Rahman Efrida Efrida ekawati, fitri elara resigia Elly Syafriani Elmatris Sy ELVI ZURIYANI Elvi Zuriyani Endang Purwati RN Endrinaldi , Endrinaldi Eryati Darwin Eryati Darwin Eryka Okta Juanty Eryka Okta Juanty FA Febria Fatchiyah Fatchiyah Febiona, Keysha Putri Febria, FA Femy Riwany Firjatullah, Muhammad F. Friska Eka Fitria Gusmawartati Gusmawartati Hamda Fauza Hanifah, Wafiq Hasbi Al Huda Hasibuan, Imron Martua Hazmi, Auliya A. Herviyanti Herviyanti Ida Parwati Ifan Aulia Candra Irawati Chaniago Irfan Suliansyah ISHAK Ishak Manti ISHAK MANTI Ishak Manti Istino Ferita Jeanne Venora Julizar Julizar Jumsu Trisno Lidya Sari Utami Lily Syukriani Lipoeto NI Liza Aulia Yusfi Maharani, Annisa D. Maulana, Rafi Meisilva Erona Sitepu Minda Azhar Mirnawati Mirnawati ML Edy Parwanto Mochammad Imron Awalludin Muhamad Irsyad Murdaningsih H. Karmana N Nasir N Nurhidayat N Nurhidayat, N Nasril Nasir Nasril Nasir Nasrul Zubir Natalia, Dessy Nefilinda Nefilinda Nefilinda Nefilinda Netty Suharti Ningsih, Azma Risilvia Noraziyah Abd Aziz Shamsudin Nur Afrainin Syah Nurainas Nurainas Nursyafi, Fauzan Syarif Obel, Obel Prayogi, Norbertus M. Putra, Andika Chandra Rachmad Hersi Martinsyah Rahmad, Ali Rahman, Awalil RK. Rahmi Handayani Rahmi Kamelia Ramadhan, Nugraha Renfiyeni Reni Koja Respati, Aditya Nugraha Retno Ariza Soeprihatini Soemarwoto Rhenly Danis Rika Despica Rika Despica Rosadi, Firsta Ninda Roslaili Rasyid Roza Yunita Rusfidra rusfidra Sahrul Utama Saibi, Ihsan R. A. Saipul Sihotang Salma Salma Salma Salma Sanusi Ibrahim Sarbaini Sarbaini Sartika, Diza Selvia Dewi Pohan Seswita, Seswita Silvia Permata Sari Slamet Rianto SRI HENDRASTUTI HIDAYAT Sumaryati Syukur Sumaryati Syukur Suswita, Rini Syamsurizal Syamsurizal Syazili Mustofa Tika Runifah Trimuri Habazar TRIMURTI HABAZAR Tristan, Christopher D. Urnemi - Urnemi Urnemi V. Maslami Vovien - Wahyudi, Dhyani P. Wardi, Epi Supri Wawan Abdullah Setiawan Winda Purnama Sari Winda Purnama Sari Y Heryandi Y. Marlida Y. S. Nur Yahono, Angela S. Yanwirasti Yanwirasti Yanwirasti Yenny, Satya W. Yuniar Lestari Yurnalis Yurnalis Yustini Alioes ZK Abdurahman Baizal Zulkarnain Chaidir