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Jamsari Jamsari
Universitas Andalas
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Physicochemical Characteristics of Gum Arabic From Acacia mearnsii Grown in Alahan Panjang, West Sumatera Syukriani, Lily; Yunita, Roza; Rosadi, Firsta Ninda; Jamsari, Jamsari
AGROSCRIPT: Journal of Applied Agricultural Sciences Vol 6 No 2 (2024): December
Publisher : Universitas Perjuangan Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/agroscript.v6i2.2049

Abstract

Acacia gum produced from Acacia mearnsii is a natural material with great potential for various industrial applications due to its unique physicochemical properties. This study aimed to characterize gum acacia from Alahan Panjang, West Sumatra. Characterizatio was done using Fourier Transform Infrared Spectroscopy (FTIR), X-ray fluorescence (XRF), and X-ray diffraction (XRD) techniques. FTIR results revealed the presence of hydroxyl (-OH), carbonyl (-C=O), and glycosidic (C-O-C) groups, indicating the complex polysaccharide structure of the gum. XRF analysis showed the dominance of calcium oxide (CaO) with a concentration of 81.652%, followed by potassium oxide (K₂O) at 10.052% and phosphate (P₂O₅) at 6.341%. Meanwhile, the XRD diffraction pattern identified a near amorphous diffraction with some weak crystalline peaks. This combination of results suggests that gum acacia has chemical and physical properties that support its use as an emulsifier, thickener, and filler in various industries, such as food, pharmaceutical, and cosmetics.
Enhanced expression of L-asparaginase II by fusion of pSSPM3 synthetic promoter into pET-28a+ expression vector for alternative targeted therapy of acute lymphoblastic leukaemia Aliya, Lisana Shiddiqin; Julizar, Julizar; Rasyid, Roslaili; Pertiwi, Dian; Syukriani, Lily; Nursyafi, Fauzan Syarif; Saibi, Ihsan R. A.; Hasibuan, Imron Martua; Febiona, Keysha Putri; Jamsari, Jamsari
Bioma : Berkala Ilmiah Biologi Volume 27 Issue 2 Year 2025
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2025.72304

Abstract

The use of commercial L-asparaginase II from E. coli and Erwinia chrysanthemi for acute lymphoblastic leukemia (ALL) therapy causes adverse effects (allergic reactions, neurotoxicity), necessitating safer alternatives. Serratia plymuthica UBCF_13, harboring the ansB gene, offers a promising source. This study constructed the recombinant plasmid pET-28a+:pSSPM3:ansB to enhance L-asparaginase II expression in E. coli using the synthetic promoter pSSPM3. Molecular verification confirmed successful steps: promoter fusion (674 bp band), ansB digestion (1,057 bp), gene insertion (1,682 bp), and BL21 transformation. Critically, enzyme activity assays revealed that pSSPM3 did not enhance expression in BL21 (0.519 U/mL), showing significantly lower activity (p<0.05) than native controls (0.621 U/mL) and DH10B transformants (0.636 U/mL). While the functional plasmid establishes a platform for novel enzyme production, the unexpected activity reduction in BL21 and higher yield in DH10B highlight host-promoter compatibility challenges. Further optimization of expression systems, purification protocols, and preclinical validation (cytotoxicity, allergenicity) are essential to advance this recombinant enzyme toward therapeutic and scalable industrial applications for ALL in resource-limited settings.
Elevation of miR-210 Expression and Mean Arterial Pressure as Early-onset Pre-eclampsia Biomarkers, while Elevation of Matrix Metalloproteinase-2 as Late-onset Pre-eclampsia Biomarker Nelvianti Nelson; Yusrawati Yusrawati; Joserizal Serudji; Jamsari Jamsari; Arni Amir; Afriwardi Afriwardi; Erry Gumilar
The Indonesian Biomedical Journal Vol 17, No 2 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i2.3490

Abstract

BACKGROUND: Pre-eclampsia has varying onset patterns, which are challenging to determine due to their association with diverse clinical parameters, including blood pressure regulation, vascular remodeling, and placental hypoxia. These parameters influence the expression of key biomarkers such as microRNAs, metalloproteinases, and arterial pressure indices. Therefore, understanding the associations is crucial for improving early diagnosis and management. This study was conducted to compare miR-210 expression, mean arterial pressure (MAP), and matrix metalloproteinase (MMP)-2 levels between early-onset pre-eclampsia (EOPE) and late-onset pre-eclampsia (LOPE).METHODS: Pregnant women with EOPE (20–34 weeks) and LOPE (35 weeks to term) were included in this cross-sectional comparative study, and their blood samples were collected. miR-210 expression was quantified with reverse transcription polymerase chain reaction (RT-PCR), MAP was measured using sphygmomanometer, while MMP-2 levels were measured using enzyme-linked immunosorbent assay (ELISA).RESULTS: miR-210 expression was more frequently observed in the EOPE group (87.5%) compared to the LOPE group (77.5%), with a significant difference identified between the two groups. The odds ratio for miR-210 expression in EOPE compared to LOPE was 2.03 (95% CI: 1.06–6.72). The MAP was notably higher in the EOPE group (121.15 mmHg) than in the LOPE group (116.15 mmHg), with a significant difference observed. Conversely, MMP-2 levels were significantly higher in the LOPE group (390.99 ng/mL) compared to the EOPE group (271.35 ng/mL).CONCLUSION: There are significant differences in miR-210 expression, MAP and MMP-2 between EOPE and LOPE. These findings suggest that miR-210, MAP, and MMP-2 could be useful biomarkers for distinguishing between EOPE and LOPE, potentially guiding more effective management and intervention strategies.KEYWORDS: miR-210, MAP, MMP-2, EOPE, LOPE, pre-eclampsia
miR-200a as Potential Early-onset Biomarker, while High Nitric Oxide as Potential Late-onset Biomarker in Preeclampsia Hudila Rifa Karmia; Yusrawati Yusrawati; Jamsari Jamsari; Hardisman Hardisman
The Indonesian Biomedical Journal Vol 17, No 3 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i3.3572

Abstract

BACKGROUND: miR-200a is known to alter trophoblast invasion and spiral artery remodeling, leading to defective placentation that causes placental hypoxia, which is the main pathomechanism in early-onset preeclampsia (EOPE). Hypoxic placentas cause systemic endothelial dysfunction that is characterized by low production of endothelial vasodilator, mainly nitric oxide (NO). On the other hand, defective placentation does not cause late-onset preeclampsia (LOPE), making the role of miR-200a expression and NO level as predictors in LOPE questionable. Therefore, this study was conducted to compare miR-200a expressions and NO levels in EOPE and LOPE to clarify their role in pathomechanism of both types of preeclampsia.METHODS: A cross-sectional comparative study was conducted in 62 preeclamptic patients (31 EOPEs and 31 LOPEs). Subjects were classified into EOPE or LOPE groups based on whether the diagnosis of preeclampsia was made at <34 or ≥34 weeks of pregnancy. miR-200a expression was analyzed using real-time polymerase chain reaction technique, and NO level was analyzed using colorimetric assay method.RESULTS: EOPE and LOPE subjects were equivalent in terms of age and parity (p=0.709 and p=0.066), but significantly difference in gestational age (p=0.000). miR-200a were expressed in 74.2% of EOPE and 41.9% of LOPE subjects (p=0.010). Median NO levels were lower in EOPE compared to LOPE subjects (23.75 vs. 106.00 µmol/L) (p=0.027), and lower in subjects with detected miR-200a compared to subjects with undetected miR-200a (62.75 vs. 132.25 µmol/L) (p=0.032).CONCLUSION: miR-200a was more expressed in EOPE compared to LOPE subjects suggesting that it might be a significant in predicting EOPE. While NO level was significantly lower in EOPE whilst higher in LOPE subjects, hence might be potential as a marker to differentiate EOPE and LOPE.KEYWORDS: miR-200a expression, NO level, early-onset preeclampsia, late-onset preeclampsia
Efektifivitas Intervensi Perubahan Perilaku Petani dalam Penggunaan Pestisida Berbasis Pendekatan Transtheoretical Model Friska Eka Fitria; Nur Afrainin Syah; Jamsari Jamsari; Yuniar Lestari
Jurnal Ners Vol. 10 No. 1 (2026): JANUARI 2026
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v10i1.52921

Abstract

Penggunaan pestisida yang tidak aman dan sehat masih menjadi persoalan serius di sektor pertanian dan meningkatkan risiko kesehatan petani. Penelitian ini bertujuan mengevaluasi efektivitas intervensi berbasis Transtheoretical Model (TTM) dalam mendorong perubahan perilaku penggunaan pestisida yang aman dan sehat pada petani hortikultura di Kabupaten Solok. Penelitian menggunakan desain one group pretest–posttest dengan 36 responden yang dipilih menggunakan quota sampling. Instrumen berupa kuesioner TTM yang memetakan lima tahap perilaku: pra-kontemplasi, kontemplasi, persiapan, tindakan, dan pemeliharaan. Intervensi diberikan satu kali melalui penyuluhan menggunakan media berupa video edukasi, diskusi kelompok dan konseling individu, dan pelatihan penggunaan APD dan praktik dosis serta pencampuran pestisida yang tepat. Hasil menunjukkan pergeseran signifikan tahap perilaku, dengan mayoritas petani bergerak dari kontemplasi ke persiapan dan tindakan. Uji Wilcoxon menghasilkan p=0,000 (p≤0,05), menandakan adanya perbedaan bermakna antara pretest dan posttest. Sebanyak 72,2% petani mengalami perubahan perilaku ke arah positif. Temuan ini menegaskan bahwa intervensi yang disesuaikan dengan tahapan kesiapan individu lebih efektif dalam mendorong perubahan perilaku, serta berpotensi menjadi dasar penyusunan strategi peningkatan kesehatan kerja petani yang berkelanjutan.
The Effect of Pesticide Exposure on Women Farmers of Reproductive Age on Growth and Development Disorders of Children Born: A Systematic Literature Review Friska Eka Fitria; Nur Afrainin Syah; Jamsari Jamsari; Yuniar Lestari
Jurnal Penelitian Pendidikan IPA Vol 9 No 9 (2023): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i9.5042

Abstract

The large number of children suffering from ADHD and autism today is a result of the increasing use of pesticides in agriculture. This applies to women farmers of childbearing age who work in agricultural settings or women whose work is related to the use of pesticides. Pesticides are a mixture of several chemicals used to control pests and protect plants. With the use of pesticides, yields obtained increased significantly. This study aims to examine the effect of exposure to pesticides on female farmers of childbearing age on growth and development disorders of children born. The review was conducted according to state-of-the-art methods using selected reporting item guidelines for reviews and meta-analyses (PRISMA). We reviewed the literature from several publications and analyzed the effect of exposure to pesticides on female farmers of childbearing age on developmental disorders of the children they were born with. The results of this study are the effect of exposure to pesticides on female farmers of childbearing age on birth defects. The use of pesticides causes pesticide poisoning. Accumulation of pesticides at a certain threshold has a negative impact on the body, namely brain disorders, tumors, cancer, even in pregnant women can cause birth defects. Apart from adults, pesticides also have a negative impact on children
ANALYSIS OF L-Asparaginase II ENZYME ACTIVITY FROM NATIVE AND RECOMBINANT Escherichia coli DH10B FOR THE APPLICATION OF ACUTE LYMPHOBLASTIC LEUKAEMIA THERAPY Keysha Putri Febiona; Dian Pertiwi; Arni Amir; Lily Syukriani; Dessy Arisanty; Almurdi Almurdi; Lisana Shiddiqin Aliya; Jamsari Jamsari
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Acute Lymphoblastic Leukemia (ALL) is a blood cancer that occurs due to abnormal proliferation of lymphoid progenitor cells. L-asparaginase II is an enzyme used to treat this disease by inducing apoptosis in cancer cells encoded by the AnsB gene. Previous studies have shown complications and toxicity in commercial L-asparaginase, necessitating exploration of L-asparaginase sources. Serratia plymuthica UBCF_13 is one of the microorganisms that encodes the AnsB gene. This study consisted of inoculation of native E. coli DH10B and E. coli DH10B pET28a(+)_AnsB, molecular validation, enzyme activity testing using direct nesslerisation method, and analysis of extracellular and intracellular protein profiles using SDS-PAGE. Enzyme activity and protein profiles were compared with S. plymuthica UBCF_13, and Isopropyl β-D-1-thiogalactopyranoside (IPTG) induction was performed on each bacteria as a comparison. The results showed the highest L-asparaginase II enzyme activity in E. coli DH10B pET28a(+)_AnsB induced with IPTG with an average enzyme activity of 0.392 ± 0.017 U/mL. Protein profile analysis using SDS-PAGE supported this finding, showing a thicker 36.5 kDa protein band compared to other bacteria. Statistical testing using One Way ANOVA showed a significant difference in L-asparaginase II enzyme activity in native E. coli DH10B and E. coli DH10B pET28a(+)_AnsB. It can be concluded that there was an increase in activity in recombinant E. coli DH10B compared to its native.
ANALYSIS OF L-Asparaginase II ENZYME ACTIVITY FROM NATIVE AND RECOMBINANT Escherichia coli DH10B FOR THE APPLICATION OF ACUTE LYMPHOBLASTIC LEUKAEMIA THERAPY Keysha Putri Febiona; Dian Pertiwi; Arni Amir; Lily Syukriani; Dessy Arisanty; Almurdi Almurdi; Lisana Shiddiqin Aliya; Jamsari Jamsari
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Acute Lymphoblastic Leukemia (ALL) is a blood cancer that occurs due to abnormal proliferation of lymphoid progenitor cells. L-asparaginase II is an enzyme used to treat this disease by inducing apoptosis in cancer cells encoded by the AnsB gene. Previous studies have shown complications and toxicity in commercial L-asparaginase, necessitating exploration of L-asparaginase sources. Serratia plymuthica UBCF_13 is one of the microorganisms that encodes the AnsB gene. This study consisted of inoculation of native E. coli DH10B and E. coli DH10B pET28a(+)_AnsB, molecular validation, enzyme activity testing using direct nesslerisation method, and analysis of extracellular and intracellular protein profiles using SDS-PAGE. Enzyme activity and protein profiles were compared with S. plymuthica UBCF_13, and Isopropyl β-D-1-thiogalactopyranoside (IPTG) induction was performed on each bacteria as a comparison. The results showed the highest L-asparaginase II enzyme activity in E. coli DH10B pET28a(+)_AnsB induced with IPTG with an average enzyme activity of 0.392 ± 0.017 U/mL. Protein profile analysis using SDS-PAGE supported this finding, showing a thicker 36.5 kDa protein band compared to other bacteria. Statistical testing using One Way ANOVA showed a significant difference in L-asparaginase II enzyme activity in native E. coli DH10B and E. coli DH10B pET28a(+)_AnsB. It can be concluded that there was an increase in activity in recombinant E. coli DH10B compared to its native.
PENGENALAN TEKNOLOGI URBAN FARMING DENGAN METODE BUDIDAYA MICROGREEN DI SMP BAITURROSYID SCHOOL KECAMATAN KOTO TANGAH, KOTA PADANG Roza Yunita; Jamsari Jamsari; Winda Purnama Sari; Meisilva Erona; Lily Syukriani; Firsta Ninda Rosadi; Jeanne Venora; Hasbi Al Huda
Jurnal Hilirisasi IPTEKS Vol. 6 No. 3 (2023)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v6i3.692

Abstract

The concept of urban farming or urban farming is farming activities in a narrow urban environment. Urban farming is a solution to produce food independently by taking advantage of limited land in urban environments. The microgreen cultivation technique is an example of farming activities with no or narrow land, harvesting time is fast and healthy. The existence of an introduction to agriculture among adolescents can foster interest in and understanding of the concept of urban agriculture. That the concept of urban farming is not like conventional farming. It is important for us to educate young people that Indonesia has abundant natural wealth, and agriculture is one of the sectors that supports the national economy. As well as building a generation of people who love agriculture. Generations that will maintain, protect and advance the agricultural sector to meet food needs in a sustainable manner. The activity aims to introduce and practice microgreen cultivation technology to Baiturrosyid Middle School students and the surrounding community so they like to grow and consume vegetables. This activity has been carried out at the Baiturossyid School Middle School, Kec. Koto Tangah, the city of Padang. The method used is socialization, counseling, demonstration of planting microgreens in containers and discussion of microgreen cultivation techniques. The conclusion of this activity is to increase students' understanding of agriculture, students can cultivate microgreens according to the way of work that is conveyed.
PENINGKATAN TEKNOLOGI BUDIDAYA STROBERI MELALUI APLIKASI EKOENZIM DAN PENGGUNAAN VARIETAS UNGGUL DI NAGARI BATANG BARUS KABUPATEN SOLOK Firsta Ninda Rosadi; Jamsari Jamsari; Roza Yunita; Reni Koja; Winda Purnama Sari; Meisilva Erona S; Lily Syukriani; Sahrul Utama; Andika Ade Saputra
Jurnal Hilirisasi IPTEKS Vol. 7 No. 2 (2024)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v7i2.789

Abstract

The Lubuek Kabau Sepakat farmer group is one of the farmer groups that actively cultivates strawberry plants. However, the strawberry production obtained is not optimal because of the lack of knowledge related to organic fertilizers and the source of planting material used is not clear about the origin of the variety because it is only vegetatively propagated from the previous plant. Therefore, the objectives of this service activity are 1) to educate farmer groups about ecoenzymes so that they can be used as one of the organic fertilizers to be applied to strawberry plants; 2) to provide seedlings of superior strawberry varieties so that the quantity and quality of strawberry production can increase. The activity was carried out at the Lubuk Kabau Sepakat Farmer Group, Nagari Batang Barus, Solok Regency, West Sumatra. The stages of activities carried out include: identification of partner needs and problems, preparation of programs that are in accordance with the needs / problems of partners, implementation of activities in accordance with the plans that have been made, then monitoring and evaluation. The result of this activity is that the partners' knowledge or skills related to ecoenzyme have increased so that they are able to independently produce ecoenzyme and will apply ecoenzyme to strawberry plants. A total of 150 strawberry seedlings of the premium Sweet Japanense variety have been delivered to partners so that the quantity and quality of strawberry production can increase. The results of the activity evaluation showed that 95% of the participants were very satisfied with the activities carried out by the service team. As many as 85% of participants said that this training activity was very useful in helping to reduce organic waste in the form of fruit peel residue. The relevance of themes and materials is in accordance with the needs and can be applied in everyday life.
Co-Authors -, Urnemi -, Vovien . Dachryanus . Herviyanti Abdi Dharma Achmad Noerkhaerin Putra Adrial Adrial, Adrial Afriwardi Afriwardi Agustian Agustian Ahmad Denian Ali Rahmad Ali Rahmat Aliya, Lisana Shiddiqin Aliya, Lisana Shidiqqin Almurdi Almurdi Alvarino Alvarino Alvarino Amir, Arni Ananda, Abi Awfa Rahman Andani Eka Putra Andika Ade Saputra Andika Ade Saputra Andika Chandra Putra Arif Sardi, Arif Armaini - Arnim Arnim Asman Manaf Azhar, Minda Azmi Dhalimi Buana, Gherriandi R. Cherlina Cherlina Cuandra, Kevin N. Dachryanus, Dachryanus Delmi Sulastri Dessy Arisanty DESSY NATALIA Dian Pertiwi Djong Hon Tjong E. Y. Rahman E. Y. Rahman Efrida Efrida ekawati, fitri elara resigia Elly Syafriani Elmatris Sy Elvi Zuriyani ELVI ZURIYANI Endang Purwati RN Endrinaldi , Endrinaldi EPI SUPRI WARDI Erry Gumilar Eryati Darwin Eryati Darwin Eryka Okta Juanty Eryka Okta Juanty Eti Yerizel FA Febria Fatchiyah Fatchiyah Febiona, Keysha Putri Febria, FA Femy Riwany Firjatullah, Muhammad F. Friska Eka Fitria Gusmawartati Hamda Fauza Hanifah, Wafiq Hardisman Hasbi Al Huda Hasbi Al Huda Hasibuan, Imron Martua Hazmi, Auliya A. HEPPY SETYA PRIMA Hudila Rifa Karmia Ida Parwati Ifan Aulia Candra Irawati Chaniago Irfan Suliansyah ISHAK Ishak Manti ISHAK MANTI Ishak Manti Istino Ferita Jeanne Venora Jeanne Venora Joserizal Serudji Julizar Julizar Jumsu Trisno Keysha Putri Febiona Lidya Sari Utami Lily Syukriani Lipoeto NI Lisana Shiddiqin Aliya Liza Aulia Yusfi Maharani, Annisa D. Maulana, Rafi Meisilva Erona Meisilva Erona Sitepu Minda Azhar Mirnawati Mirnawati ML Edy Parwanto Mochammad Imron Awalludin Muhamad Irsyad Murdaningsih H. Karmana Musliar Kasim N Nasir N Nurhidayat N Nurhidayat, N Nasril Nasir Nasril Nasir Nasrul Zubir Natalia, Dessy Nefilinda Nefilinda Nefilinda Nefilinda Nelvianti Nelson Netty Suharti Ningsih, Azma Risilvia Noraziyah Abd Aziz Shamsudin Nur Afrainin Syah Nur Indrawaty Lipoeto Nurainas Nurainas Nursyafi, Fauzan Syarif Obel, Obel Prambudi Rukmono Prayogi, Norbertus Marcell Prima Minerva Rachmad Hersi Martinsyah Rahmad, Ali Rahman, Awalil RK. Rahmi Handayani Rahmi Kamelia Ramadhan, Nugraha Renfiyeni Reni Koja Respati, Aditya Nugraha Retno Ariza Soeprihatini Soemarwoto Rhenly Danis Rika Despica Rika Despica Roslaili Rasyid Roza Yunita Rusfidra rusfidra Sahrul Utama Sahrul Utama Saibi, Ihsan R. A. Saipul Sihotang Salma Salma Salma Salma Sanusi Ibrahim Sarbaini Sarbaini Sartika, Diza SELVIA DEWI POHAN Selvia Dewi Pohan Seswita, Seswita Silvia Permata Sari Slamet Rianto SRI HENDRASTUTI HIDAYAT SUMARYATI SYUKUR Sumaryati Syukur Sumaryati Syukur Suswita, Rini Syamsurizal Syamsurizal Syazili Mustofa TIKA RUNIFAH Tika Runifah Tofrizal Tofrizal Trimuri Habazar TRIMURTI HABAZAR Tristan, Christopher D. Urnemi - Urnemi Urnemi V. Maslami Vovien - Wahyudi, Dhyani P. Wardi, Epi Supri Wawan Abdullah Setiawan Winda Purnama Sari Winda Purnama Sari Y Heryandi Y. Marlida Y. S. Nur Yahono, Angela S. Yanwirasti Yanwirasti Yanwirasti YASWENDRI YASWENDRI Yenny, Satya W. Yuniar Lestari Yurnalis Yurnalis Yusrawati Yusrawati Yustini Alioes ZK Abdurahman Baizal ZULKARNAIN CHAIDIR Zulkarnain Chaidir