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Lactococcus lactis D4 Has Potential Effect to Alleviate Inflammation and Reverse Dysbiosis in Colitis Rat Model Suswita, Rini; Alvarino, Alvarino; Darwin, Eryati; Jamsari, Jamsari
The Indonesian Biomedical Journal Vol 16, No 2 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i2.2970

Abstract

BACKGROUND: Inflammatory bowel disease (IBD) is a prevalent chronic inflammatory disorder of the gastrointestinal tract that may lead to colorectal cancer if untreated. Several medications for IBD have adverse side effects. Thus, there are high interest for searching alternative treatment to treat IBD. Lactococcus lactis D4 isolated from dadih, a traditional fermented buffalo milk product, was investigated for the effect on gut inflammation and microbial composition in the colitis rat model.METHODS: Eighteen male Sprague-Dawley rats were divided into three groups; control rats, colitis-induced rats, and colitis-induced rats treated with L. lactis D4 isolate. The control group received water, while the colitis-induced rats were given azoxymethane (AOM) and dextran sodium sulphate (DSS). Rats’ feces were collected for the analysis of gut microbiota diversity by next-generation sequencing and for the measurement of transforming growth factor (TGF)-β, nuclear factor kappa-B (NF-κB), interleukin (IL)-6, and tumor necrosis factor (TNF)-α colonic expression using reverse transcription-quantitative polymerase chain reaction (RT-qPCR).RESULTS: L. lactis D4 administration was able to reduce inflammation in colitis by decreasing IL-6 expression (0.87 vs. 0.73), while expression of TGF-β, NF-κB, and TNF-α were increased compared to the model group, suggesting a complex immunomodulatory effect. Gut microbiota sequencing revealed a similarity between the control and treatment groups, suggesting L. lactis D4 has great potential to ameliorate gut microbiota dysbiosis in colitis rats.CONCLUSION: L. lactis D4 has a beneficial effect in decreasing pro-inflammatory cytokines and is able to reserve dysbiosis in colitis rat model. Thus, L. lactis D4 might be used as a therapeutic agent for IBD.KEYWORDS: colitis, gut microbiota, inflammation, inflammatory bowel disease, Lactococcus lactis D4, probiotic, proinflammatory cytokines
Exploring the Therapeutic Landscape: A Systematic Review on the Anti-inflammatory Effects of Probiotics in Colitis-associated Colorectal Cancer Suswita, Rini; Alvarino; Eryati Darwin; Jamsari
Majalah Kedokteran Andalas Vol. 47 No. 3 (2024): MKA July 2024
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v47.i3.p341-361.2024

Abstract

Background: Colorectal cancer is closely linked to chronic inflammation; long-standing colitis confers a 2–3-fold higher risk. Modulating the gut–immune axis with probiotics may attenuate the inflammatory microenvironment of colitis-associated colorectal cancer (CAC). Objective:To evaluate the anti-inflammatory effects of probiotics in CAC animal models. Methods: We searched PubMed, ScienceDirect, and Wiley Online Library; 248 records were screened and 9 studies met inclusion. Risk of bias was appraised using SYRCLE RoB. Results: Probiotic treatment consistently reduced clinical severity, down-regulated inflammatory markers, and modulated key signaling pathways in CAC models. Nonetheless, evidence was heterogeneous, sample sizes were small, and dosing, strains, and formulations varied; safety reporting was limited. Conclusions: Probiotics show promise as anti-inflammatory adjuncts in CAC based on animal studies. Rigorous, standardized preclinical work and well-designed trials are needed to define optimal strains, dosing, and formulations, and to confirm efficacy and safety for translation.
Dual Infection of Pepper Yellow Leaf Curl Virus and Chilli Veinal Mottle Virus in Causing the Yellow leaf Curl Disease on Chili Trisno, Jumsu; Jamsari, Jamsari; Hidayat, Sri Hendrastuti
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 5 No. 2 (2021): December 2021
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.5.2.77-88.2021

Abstract

Yellow leaf curl disease is the primary disease in chili plants with up to 100% losses. Detection from the field showed a mixed infection between Geminivirus or Pepper Yellow Leaf Curl Virus (PYLCV) and several viruses, including Chilli Veinal Mottle Virus (ChiVMV) and Cucumber mosaic Virus (CMV). Therefore, this study aimed to determine the role of PYLCV and ChiVMV dual infection in influencing the development of chili yellow leaf curl disease. This study used a completely randomized design (CRD) with six treatments and ten replications. The treatments used were multiple Geminiviruses and ChiVMV infections simultaneously, before ChiVMV co-infection, after Geminivirus infection, single infection and whithout infection viruses. The results showed that the presence of ChiVMV infection before, after, and concurrently with Geminiviruses infection could increase the yellow leaf curl disease intensity and reduce plant height and wet weight growth. The mean scale value of ChiVMV infection before, after, and concurrently with Geminiviruses infection were 3.38, 3.90 and 3.58 compared to single Geminiviruses infection (scale of 3.20) and single ChiVMV (scale of 1.33). The interaction analysis of ChiVMV and geminiviruses infections based on the percentage reduction in plant growth height and wet weight showed additive interaction. ChiVMV co-infection further exacerbated the incidence of yellow leaf curl diseases in chilies.
The Effect of Pesticide Exposure on Women Farmers of Reproductive Age on Growth and Development Disorders of Children Born: A Systematic Literature Review Fitria, Friska Eka; Syah, Nur Afrainin; Jamsari, Jamsari; Lestari, Yuniar
Jurnal Penelitian Pendidikan IPA Vol 9 No 9 (2023): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i9.5042

Abstract

The large number of children suffering from ADHD and autism today is a result of the increasing use of pesticides in agriculture. This applies to women farmers of childbearing age who work in agricultural settings or women whose work is related to the use of pesticides. Pesticides are a mixture of several chemicals used to control pests and protect plants. With the use of pesticides, yields obtained increased significantly. This study aims to examine the effect of exposure to pesticides on female farmers of childbearing age on growth and development disorders of children born. The review was conducted according to state-of-the-art methods using selected reporting item guidelines for reviews and meta-analyses (PRISMA). We reviewed the literature from several publications and analyzed the effect of exposure to pesticides on female farmers of childbearing age on developmental disorders of the children they were born with. The results of this study are the effect of exposure to pesticides on female farmers of childbearing age on birth defects. The use of pesticides causes pesticide poisoning. Accumulation of pesticides at a certain threshold has a negative impact on the body, namely brain disorders, tumors, cancer, even in pregnant women can cause birth defects. Apart from adults, pesticides also have a negative impact on children
Physicochemical Characteristics of Gum Arabic From Acacia mearnsii Grown in Alahan Panjang, West Sumatera Syukriani, Lily; Yunita, Roza; Rosadi, Firsta Ninda; Jamsari, Jamsari
AGROSCRIPT: Journal of Applied Agricultural Sciences Vol 6 No 2 (2024): December
Publisher : Universitas Perjuangan Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/agroscript.v6i2.2049

Abstract

Acacia gum produced from Acacia mearnsii is a natural material with great potential for various industrial applications due to its unique physicochemical properties. This study aimed to characterize gum acacia from Alahan Panjang, West Sumatra. Characterizatio was done using Fourier Transform Infrared Spectroscopy (FTIR), X-ray fluorescence (XRF), and X-ray diffraction (XRD) techniques. FTIR results revealed the presence of hydroxyl (-OH), carbonyl (-C=O), and glycosidic (C-O-C) groups, indicating the complex polysaccharide structure of the gum. XRF analysis showed the dominance of calcium oxide (CaO) with a concentration of 81.652%, followed by potassium oxide (K₂O) at 10.052% and phosphate (P₂O₅) at 6.341%. Meanwhile, the XRD diffraction pattern identified a near amorphous diffraction with some weak crystalline peaks. This combination of results suggests that gum acacia has chemical and physical properties that support its use as an emulsifier, thickener, and filler in various industries, such as food, pharmaceutical, and cosmetics.
Co-Authors -, Urnemi -, Vovien . Dachryanus Abdi Dharma Achmad Noerkhaerin Putra Adrial Adrial, Adrial Afriwardi Afriwardi Agustian Agustian Ahmad Denian Ali Rahmad Ali Rahmat Aliya, Lisana Shidiqqin Alvarino Alvarino Alvarino Ananda, Abi Awfa Rahman Andani Eka Putra Andika Ade Saputra Arif Sardi Armaini - Arni Amir Arnim Arnim Asman Manaf Azhar, Minda Azmi Dhalimi Buana, Gherriandi R. Cuandra, Kevin N. Dachryanus, Dachryanus Delmi Sulastri DESSY NATALIA Djong Hon Tjong E. Y. Rahman E. Y. Rahman Efrida Efrida ekawati, fitri elara resigia Elly Syafriani Elmatris , Elmatris ELVI ZURIYANI Elvi Zuriyani Endang Purwati RN Endrinaldi , Endrinaldi Eryati Darwin Eryati Darwin Eryka Okta Juanty Eryka Okta Juanty FA Febria Fatchiyah Fatchiyah Febria, FA Femy Riwany Firjatullah, Muhammad F. Gumilar, Erry Gusmawartati Gusmawartati Hamda Fauza Hanifah, Wafiq Hardisman Hasbi Al Huda Hasibuan, Imron Martua Hazmi, Auliya A. Herviyanti Herviyanti Ida Parwati Ifan Aulia Candra Irawati Chaniago Irfan Suliansyah ISHAK ISHAK MANTI Ishak Manti Ishak Manti Istino Ferita Jeanne Venora Jumsu Trisno Karmia, Hudila Rifa Lidya Sari Utami Lily Syukriani Lipoeto NI Liza Aulia Yusfi Maharani, Annisa D. Maulana, Rafi Meisilva Erona Meisilva Erona S Minda Azhar Mirnawati Mirnawati ML Edy Parwanto Mochammad Imron Awalludin Muhamad Irsyad Murdaningsih H. Karmana N Nasir N Nurhidayat N Nurhidayat, N Nasril Nasir Nasril Nasir Nasrul Zubir Natalia, Dessy Nefilinda Nefilinda Nefilinda Nefilinda Nelson, Nelvianti Netty Suharti Ningsih, Azma Risilvia Noraziyah Abd Aziz Shamsudin Nur Afrainin Syah Nurainas Nurainas Nursyafi, Fauzan Syarif Obel Obel Obel, Obel Prayogi, Norbertus M. Putra, Andika Chandra Rachmad Hersi Martinsyah Rahmad, Ali Rahman, Awalil RK. Rahmi Handayani Rahmi Kamelia Ramadhan, Nugraha Renfiyeni Reni Koja Respati, Aditya Nugraha Retno Ariza Soeprihatini Soemarwoto Rhenly Danis Rika Despica Rika Despica Rosadi, Firsta Ninda Roza Yunita Rusfidra rusfidra Sahrul Utama Saipul Sihotang Salma Salma Salma Salma Sanusi Ibrahim Sarbaini Sarbaini Sartika, Diza Selvia Dewi Pohan Seswita, Seswita Silvia Permata sari Slamet Rianto SRI HENDRASTUTI HIDAYAT Sumaryati Syukur Sumaryati Syukur Suswita, Rini Syamsurizal Syamsurizal Syazili Mustofa Tika Runifah Trimuri Habazar TRIMURTI HABAZAR Tristan, Christopher D. Urnemi - Urnemi Urnemi V. Maslami Vovien - Wahyudi, Dhyani P. Wardi, Epi Supri Wawan Abdullah Setiawan Winda Purnama Sari Winda Purnama Sari Y Heryandi Y. Marlida Y. S. Nur Yahono, Angela S. Yanwirasti Yanwirasti Yanwirasti Yenny, Satya W. Yuniar Lestari Yurnalis Yurnalis Yusrawati Yusrawati Yustini Alioes ZK Abdurahman Baizal Zulkarnain Chaidir