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All Journal CHEMISTRY PROGRESS MEDIA MEDIKA INDONESIANA Bioma : Berkala Ilmiah Biologi Zuriat Jurnal Natur Indonesia Jurnal Agrista Microbiology Indonesia Journal of Tropical Soils Plumula : Berkala Ilmiah Agroteknologi Jurnal Sain Peternakan Indonesia Jurnal Peternakan Indonesia Jurnal Riset Kimia Jurnal Agroekoteknologi Indonesian Journal of Biotechnology BERITA BIOLOGI The Indonesian Biomedical Journal JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Universa Medicina JURNAL GALUNG TROPIKA As-syifaa Jurnal Farmasi Jurnal Penelitian Pendidikan IPA (JPPIPA) Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) JURNAL AGRONIDA Jurnal Hilirisasi IPTEKS (JHI) Buletin Ilmiah Nagari Membangun Jerami Eksakta : Berkala Ilmiah Bidang MIPA Majalah Kedokteran Andalas Jurnal Agronomi Tanaman Tropika (JUATIKA) International Journal of Agricultural Sciences Jurnal Ners Jurnal Riset Teknologi Industri Unri Conference Series: Community Engagement Indian Journal of Forensic Medicine & Toxicology Narra J Pancaran Pendidikan Rafflesia Journal of Natural and Applied Sciences (RJNAS) Bioma : Jurnal Ilmiah Biologi Agroscript: Journal of Applied Agricultural Sciences Indonesian Journal of Global Health Research
Jamsari Jamsari
Universitas Andalas
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Physicochemical Characteristics of Gum Arabic From Acacia mearnsii Grown in Alahan Panjang, West Sumatera Syukriani, Lily; Yunita, Roza; Rosadi, Firsta Ninda; Jamsari, Jamsari
AGROSCRIPT: Journal of Applied Agricultural Sciences Vol 6 No 2 (2024): December
Publisher : Universitas Perjuangan Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/agroscript.v6i2.2049

Abstract

Acacia gum produced from Acacia mearnsii is a natural material with great potential for various industrial applications due to its unique physicochemical properties. This study aimed to characterize gum acacia from Alahan Panjang, West Sumatra. Characterizatio was done using Fourier Transform Infrared Spectroscopy (FTIR), X-ray fluorescence (XRF), and X-ray diffraction (XRD) techniques. FTIR results revealed the presence of hydroxyl (-OH), carbonyl (-C=O), and glycosidic (C-O-C) groups, indicating the complex polysaccharide structure of the gum. XRF analysis showed the dominance of calcium oxide (CaO) with a concentration of 81.652%, followed by potassium oxide (K₂O) at 10.052% and phosphate (P₂O₅) at 6.341%. Meanwhile, the XRD diffraction pattern identified a near amorphous diffraction with some weak crystalline peaks. This combination of results suggests that gum acacia has chemical and physical properties that support its use as an emulsifier, thickener, and filler in various industries, such as food, pharmaceutical, and cosmetics.
Enhanced expression of L-asparaginase II by fusion of pSSPM3 synthetic promoter into pET-28a+ expression vector for alternative targeted therapy of acute lymphoblastic leukaemia Aliya, Lisana Shiddiqin; Julizar, Julizar; Rasyid, Roslaili; Pertiwi, Dian; Syukriani, Lily; Nursyafi, Fauzan Syarif; Saibi, Ihsan R. A.; Hasibuan, Imron Martua; Febiona, Keysha Putri; Jamsari, Jamsari
Bioma : Berkala Ilmiah Biologi Volume 27 Issue 2 Year 2025
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2025.72304

Abstract

The use of commercial L-asparaginase II from E. coli and Erwinia chrysanthemi for acute lymphoblastic leukemia (ALL) therapy causes adverse effects (allergic reactions, neurotoxicity), necessitating safer alternatives. Serratia plymuthica UBCF_13, harboring the ansB gene, offers a promising source. This study constructed the recombinant plasmid pET-28a+:pSSPM3:ansB to enhance L-asparaginase II expression in E. coli using the synthetic promoter pSSPM3. Molecular verification confirmed successful steps: promoter fusion (674 bp band), ansB digestion (1,057 bp), gene insertion (1,682 bp), and BL21 transformation. Critically, enzyme activity assays revealed that pSSPM3 did not enhance expression in BL21 (0.519 U/mL), showing significantly lower activity (p<0.05) than native controls (0.621 U/mL) and DH10B transformants (0.636 U/mL). While the functional plasmid establishes a platform for novel enzyme production, the unexpected activity reduction in BL21 and higher yield in DH10B highlight host-promoter compatibility challenges. Further optimization of expression systems, purification protocols, and preclinical validation (cytotoxicity, allergenicity) are essential to advance this recombinant enzyme toward therapeutic and scalable industrial applications for ALL in resource-limited settings.
Efektifivitas Intervensi Perubahan Perilaku Petani dalam Penggunaan Pestisida Berbasis Pendekatan Transtheoretical Model Friska Eka Fitria; Nur Afrainin Syah; Jamsari Jamsari; Yuniar Lestari
Jurnal Ners Vol. 10 No. 1 (2026): JANUARI 2026
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v10i1.52921

Abstract

Penggunaan pestisida yang tidak aman dan sehat masih menjadi persoalan serius di sektor pertanian dan meningkatkan risiko kesehatan petani. Penelitian ini bertujuan mengevaluasi efektivitas intervensi berbasis Transtheoretical Model (TTM) dalam mendorong perubahan perilaku penggunaan pestisida yang aman dan sehat pada petani hortikultura di Kabupaten Solok. Penelitian menggunakan desain one group pretest–posttest dengan 36 responden yang dipilih menggunakan quota sampling. Instrumen berupa kuesioner TTM yang memetakan lima tahap perilaku: pra-kontemplasi, kontemplasi, persiapan, tindakan, dan pemeliharaan. Intervensi diberikan satu kali melalui penyuluhan menggunakan media berupa video edukasi, diskusi kelompok dan konseling individu, dan pelatihan penggunaan APD dan praktik dosis serta pencampuran pestisida yang tepat. Hasil menunjukkan pergeseran signifikan tahap perilaku, dengan mayoritas petani bergerak dari kontemplasi ke persiapan dan tindakan. Uji Wilcoxon menghasilkan p=0,000 (p≤0,05), menandakan adanya perbedaan bermakna antara pretest dan posttest. Sebanyak 72,2% petani mengalami perubahan perilaku ke arah positif. Temuan ini menegaskan bahwa intervensi yang disesuaikan dengan tahapan kesiapan individu lebih efektif dalam mendorong perubahan perilaku, serta berpotensi menjadi dasar penyusunan strategi peningkatan kesehatan kerja petani yang berkelanjutan.
The Effect of Pesticide Exposure on Women Farmers of Reproductive Age on Growth and Development Disorders of Children Born: A Systematic Literature Review Friska Eka Fitria; Nur Afrainin Syah; Jamsari Jamsari; Yuniar Lestari
Jurnal Penelitian Pendidikan IPA Vol 9 No 9 (2023): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i9.5042

Abstract

The large number of children suffering from ADHD and autism today is a result of the increasing use of pesticides in agriculture. This applies to women farmers of childbearing age who work in agricultural settings or women whose work is related to the use of pesticides. Pesticides are a mixture of several chemicals used to control pests and protect plants. With the use of pesticides, yields obtained increased significantly. This study aims to examine the effect of exposure to pesticides on female farmers of childbearing age on growth and development disorders of children born. The review was conducted according to state-of-the-art methods using selected reporting item guidelines for reviews and meta-analyses (PRISMA). We reviewed the literature from several publications and analyzed the effect of exposure to pesticides on female farmers of childbearing age on developmental disorders of the children they were born with. The results of this study are the effect of exposure to pesticides on female farmers of childbearing age on birth defects. The use of pesticides causes pesticide poisoning. Accumulation of pesticides at a certain threshold has a negative impact on the body, namely brain disorders, tumors, cancer, even in pregnant women can cause birth defects. Apart from adults, pesticides also have a negative impact on children
Literatur Review: The Role of Probiotics in Preventing Aging Prima Minerva; Nur Indrawaty Lipoeto; Jamsari Jamsari; Tofrizal Tofrizal
Indonesian Journal of Global Health Research Vol. 8 No. 1 (2026): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v8i1.1391

Abstract

Aging is a complex biological process characterized by progressive functional decline of organs and tissues, largely driven by oxidative stress, chronic inflammation, and cellular senescence. Recent evidence suggests that age-related gut microbiota dysbiosis contributes significantly to inflammaging and oxidative damage, accelerating the aging process. Probiotics have emerged as potential gerobiotics due to their antioxidant, anti-inflammatory, and microbiota-modulating properties. This literature review aims to evaluate the role of probiotics in preventing and delaying the aging process through modulation of gut microbiota, oxidative stress reduction, and regulation of inflammatory. A narrative literature review was conducted using scientific publications from 2012 to 2023 obtained from NCBI (PubMed), ScienceDirect, and Google Scholar databases. Articles were selected based on relevance to probiotics, gut microbiota, oxidative stress, inflammation, and aging mechanisms. A total of 300 articles were identified from PubMed (n = 120), Science Direct (n = 95), and Google Scholar (n = 85). After removing duplicates, 235 articles were screened. 45 full text articles were assessed for eligibility, and 11 studies were included in the quantitative synthesis. The reviewed studies indicate that probiotics, particularly strains from the genera Lactobacillus, exert anti-aging effects by reducing reactive oxygen species (ROS), enhancing endogenous antioxidant enzymes (SOD, CAT, GPx), regulating inflammatory pathways (NF-κB, cytokines), activating Nrf2 signaling, maintaining intestinal barrier integrity, and restoring gut microbiota balance. Several probiotic strains were shown to delay telomere shortening, suppress cellular senescence markers, and improve metabolic. Probiotics play a significant role in preventing aging by modulating gut microbiota composition, reducing oxidative stress, and suppressing chronic inflammation. These findings support the potential application of probiotics as gerobiotics to promote healthy aging and extend health span.
Hubungan Polimorfisme Gen Human Defensin β 1 rs11362 dan rs1799946 dengan Kejadian Sepsis Neonatorum Awitan Lambat : Sebuah Studi Komparatif Cross-Sectional Cherlina Cherlina; Jamsari Jamsari; Eti Yerizel; Prambudi Rukmono
Jurnal Ners Vol. 10 No. 3 (2026): JULI 2026
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v10i3.60769

Abstract

Sepsis neonatorum merupakan salah satu penyebab utama morbiditas dan mortalitas bayi baru lahir yang diduga dipengaruhi oleh faktor genetik pada gen-gen yang berperan dalam sistem imun bawaan. Human Defensin β 1 (HDB1) merupakan peptida antimikroba yang berperan penting dalam pertahanan awal tubuh terhadap infeksi. Penelitian ini bertujuan menganalisis hubungan antara polimorfisme gen HDB1 pada lokus rs11362 dan rs1799946 dengan kejadian sepsis neonatorum. Penelitian observasional dengan desain cross-sectional comparative ini menggunakan 60 sampel DNA genomik arsip yang telah tersimpan sebelumnya, terdiri atas 30 sampel neonatus dengan sepsis neonatorum awitan lambat (onset >72 jam dengan kultur darah positif) dan 30 neonatus tanpa sepsis. Genotipe kedua lokus ditentukan melalui amplifikasi PCR dengan sepasang primer yang mengapit kedua lokus (ukuran amplikon 833 bp), dilanjutkan sekuensing Sanger, kemudian dianalisis menggunakan uji chi-square pada derajat kepercayaan 95%. Hasil penelitian menunjukkan hubungan yang bermakna antara polimorfisme HDB1 rs11362 C>T dengan kejadian sepsis neonatorum (p=0,043), dimana dari seluruh subjek yang memiliki genotipe TT rs11362, sebanyak 15 dari 21 subjek (71,4%) merupakan kelompok sepsis, sedangkan 6 dari 21 subjek (28,6%) berasal dari kelompok tanpa sepsis. Sebaliknya, polimorfisme HDB1 rs1799946 C>T tidak menunjukkan hubungan yang bermakna dengan kejadian sepsis neonatorum (p=0,349). Dapat disimpulkan bahwa polimorfisme gen HDB1 rs11362, tetapi tidak rs1799946, berhubungan dengan kerentanan neonatus terhadap sepsis, menunjukkan potensinya sebagai kandidat biomarker genetik untuk diteliti dan dikembangkan lebih lanjut.
Co-Authors -, Urnemi -, Vovien . Dachryanus . Herviyanti Abdi Dharma Achmad Noerkhaerin Putra Adrial Adrial, Adrial Agustian Agustian Ahmad Denian Ali Rahmad Ali Rahmat Aliya, Lisana Shiddiqin Aliya, Lisana Shidiqqin Alvarino Alvarino Alvarino Ananda, Abi Awfa Rahman Andani Eka Putra Andika Ade Saputra Arif Sardi, Arif Armaini - Arnim Arnim Asman Manaf Azhar, Minda Azmi Dhalimi Buana, Gherriandi R. Cherlina Cherlina Cuandra, Kevin N. Dachryanus, Dachryanus Delmi Sulastri DESSY NATALIA Dian Pertiwi Djong Hon Tjong E. Y. Rahman E. Y. Rahman Efrida Efrida ekawati, fitri elara resigia Elly Syafriani Elmatris Sy Elvi Zuriyani ELVI ZURIYANI Endang Purwati RN Endrinaldi , Endrinaldi Eryati Darwin Eryati Darwin Eryka Okta Juanty Eryka Okta Juanty Eti Yerizel FA Febria Fatchiyah Fatchiyah Febiona, Keysha Putri Febria, FA Femy Riwany Firjatullah, Muhammad F. Friska Eka Fitria Gusmawartati Gusmawartati Hamda Fauza Hanifah, Wafiq Hasbi Al Huda Hasibuan, Imron Martua Hazmi, Auliya A. Ida Parwati Ifan Aulia Candra Irawati Chaniago Irfan Suliansyah ISHAK ISHAK MANTI Ishak Manti Ishak Manti Istino Ferita Jeanne Venora Julizar Julizar Jumsu Trisno Lidya Sari Utami Lily Syukriani Lipoeto NI Liza Aulia Yusfi Maharani, Annisa D. Maulana, Rafi Meisilva Erona Sitepu Minda Azhar Mirnawati Mirnawati ML Edy Parwanto Mochammad Imron Awalludin Muhamad Irsyad Murdaningsih H. Karmana N Nasir N Nurhidayat N Nurhidayat, N Nasril Nasir Nasril Nasir Nasrul Zubir Natalia, Dessy Nefilinda Nefilinda Nefilinda Nefilinda Netty Suharti Ningsih, Azma Risilvia Noraziyah Abd Aziz Shamsudin Nur Afrainin Syah Nur Indrawaty Lipoeto Nurainas Nurainas Nursyafi, Fauzan Syarif Obel, Obel Prambudi Rukmono Prayogi, Norbertus Marcell Prima Minerva Putra, Andika Chandra Rachmad Hersi Martinsyah Rahmad, Ali Rahman, Awalil RK. Rahmi Handayani Rahmi Kamelia Ramadhan, Nugraha Renfiyeni Reni Koja Respati, Aditya Nugraha Retno Ariza Soeprihatini Soemarwoto Rhenly Danis Rika Despica Rika Despica Roslaili Rasyid Roza Yunita Rusfidra rusfidra Sahrul Utama Saibi, Ihsan R. A. Saipul Sihotang Salma Salma Salma Salma Sanusi Ibrahim Sarbaini Sarbaini Sartika, Diza Selvia Dewi Pohan Seswita, Seswita Silvia Permata Sari Slamet Rianto SRI HENDRASTUTI HIDAYAT Sumaryati Syukur Sumaryati Syukur Suswita, Rini Syamsurizal Syamsurizal Syazili Mustofa Tika Runifah Tofrizal Tofrizal Trimuri Habazar TRIMURTI HABAZAR Tristan, Christopher D. Urnemi - Urnemi Urnemi V. Maslami Vovien - Wahyudi, Dhyani P. Wardi, Epi Supri Wawan Abdullah Setiawan Winda Purnama Sari Winda Purnama Sari Y Heryandi Y. Marlida Y. S. Nur Yahono, Angela S. Yanwirasti Yanwirasti Yanwirasti Yenny, Satya W. Yuniar Lestari Yurnalis Yurnalis Yustini Alioes ZK Abdurahman Baizal Zulkarnain Chaidir