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Jamsari Jamsari
Universitas Andalas
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Elimination of ineffective inorganic salt component in medium for indole‐3‐acetic acid synthesis by Serratia plymuthica UBCF_13 and its effect on the growth of chili seedlings Liza Aulia Yusfi; Djong Hon Tjong; Irawati Chaniago; Muhamad Irsyad; Jamsari Jamsari
Indonesian Journal of Biotechnology Vol 29, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.88774

Abstract

Indole‐3‐acetic acid (IAA) is an essential phytohormone that controls a variety of plant growth mechanisms. Bacteria can produce IAA to stimulate plant growth, with its production influenced by the culture conditions. Serratia plymuthica UBCF_13 is recognized as an IAA‐producing bacterium, exhibiting maximum IAA production in a yeast medium comprising yeast extract, sucrose, K2HPO4, MgSO4, NaCl, and CaCO3. However, prior studies optimizing individual inorganic salt components indicated minimal impact on IAA synthesis within this medium. This study aimed to eliminate the unnecessary inorganic salt components and the medium was then applied to investigate the IAA biosynthesis pathway and the plant growth‐promoting assay. The elimination assay consisted of yeast sucrose medium devoid of K2HPO4, MgSO4, NaCl, or CaCO3, and yeast sucrose medium containing only MgSO4 and CaCO3. Various indole compounds were then added to the revised medium composition to investigate the IAA biosynthesis pathway of UBCF_13 using high‐performance liquid chromatography (HPLC). Furthermore, the effect of UBCF_13 culture supernatant, cultivated in the new medium, on chili plant growth was evaluated. The highest IAA production (138.8 µg/mL) was observed in the yeast sucrose with CaCO3 and MgSO4 (elimination of K2HPO4 and NaCl). The presence of indole‐3‐acetamide (IAM) compound from the medium extracts, supplemented with multiple indole compounds, revealed that UBCF_13 may use the IAM pathway. The application of UBCF_13 supernatant enhanced the shoot, root length, fresh weight, and germination time of chili seeds by 37.7%, 49.3%, 204.3%, and 38.6%, respectively. This study demonstrated that eliminating K2HPO4 and NaCl provided a new culture medium composition conducive to IAA production by UBCF_13. Moreover, the UBCF_13 extract has the potential to promote plant growth.
Desain Primer Dan Deteksi Gen CHS (chalcone synthase) Pada Tanaman Gambir (Uncaria gambir (Hunter) Roxb.) Tipe Riau Gadang Epi Supri Wardi; Sumaryati Syukur; Zulkarnain Chaidir; Jamsari Jamsari; Diza Sartika
RAFFLESIA JOURNAL OF NATURAL AND APPLIED SCIENCES Vol. 1 No. 1 (2021): April
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/rjna.v1i1.15591

Abstract

Gambir merupakan salah satu tanaman yang mengandung katekin.Kandungan katekin pada gambir merupakan komponen yang menjadi syarat utama dalam penentuan mutu gambir. CHS (Chalcone synthase) adalah salah satu gen yang terlibat di dalam proses biosintesis pembentukan katekin. Penelitian ini bertujuan untuk mendapatkan primer yang dapat digunakan dalam deteksi gen CHS (Chalcone synthase) pada tanaman gambir tipe riau gadang serta untuk melihat kemampuan primer yang telah didesain untuk mendeteksi gen CHS pada tanaman gambir. Pendesainan primer dilakukan dengan mengalignment 21 data sekuengen CHS dan dipilih daerah yang memiliki kesamaan basa antara sekuen gen yang dialignment untuk mendapatkan primer.  Isolasi DNA gen CHS tanaman gambir menggunakan metode CTAB dan untuk isolasi RNA menggunakan Total RNA Mini Kit (Plant) dari Geneaid. Sintesis cDNA menggunakan kit Rever Tra Ace® qPCR RT Master Mix with gDNA Remover (Toyobo).Pada hasil desain primer didapatkan empat primer forward dan satu primer reverse. Hasil desain primer yang dapat digunakan untuk deteksi gen CHS dengan hasil cDNA tanaman gambir yaitu primer F3-R1(TNGTCTTCTGCACNACCTCCGGNG - CCANTC CAASCCYTCWCCDGTSGT). Proses deteksi gen CHS pada daun gambir menghasilkan produk dengan estimasi sebesar 724 bp
PENINGKATAN TEKNOLOGI BUDIDAYA STROBERI MELALUI APLIKASI EKOENZIM DAN PENGGUNAAN VARIETAS UNGGUL DI NAGARI BATANG BARUS KABUPATEN SOLOK Firsta Ninda Rosadi; Jamsari Jamsari; Roza Yunita; Reni Koja; Winda Purnama Sari; Meisilva Erona S; Lily Syukriani; Sahrul Utama; Andika Ade Saputra
Jurnal Hilirisasi IPTEKS Vol 7 No 2 (2024)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v7i2.789

Abstract

The Lubuek Kabau Sepakat farmer group is one of the farmer groups that actively cultivates strawberry plants. However, the strawberry production obtained is not optimal because of the lack of knowledge related to organic fertilizers and the source of planting material used is not clear about the origin of the variety because it is only vegetatively propagated from the previous plant. Therefore, the objectives of this service activity are 1) to educate farmer groups about ecoenzymes so that they can be used as one of the organic fertilizers to be applied to strawberry plants; 2) to provide seedlings of superior strawberry varieties so that the quantity and quality of strawberry production can increase. The activity was carried out at the Lubuk Kabau Sepakat Farmer Group, Nagari Batang Barus, Solok Regency, West Sumatra. The stages of activities carried out include: identification of partner needs and problems, preparation of programs that are in accordance with the needs / problems of partners, implementation of activities in accordance with the plans that have been made, then monitoring and evaluation. The result of this activity is that the partners' knowledge or skills related to ecoenzyme have increased so that they are able to independently produce ecoenzyme and will apply ecoenzyme to strawberry plants. A total of 150 strawberry seedlings of the premium Sweet Japanense variety have been delivered to partners so that the quantity and quality of strawberry production can increase. The results of the activity evaluation showed that 95% of the participants were very satisfied with the activities carried out by the service team. As many as 85% of participants said that this training activity was very useful in helping to reduce organic waste in the form of fruit peel residue. The relevance of themes and materials is in accordance with the needs and can be applied in everyday life.
Weed Vegetation Analysis and Response to Sunflower Extracts in the Uplands Winda Purnama Sari; Fitri Ekawati; Jamsari Jamsari
JERAMI : Indonesian Journal of Crop Science Vol 6 No 2 (2024): JIJCS
Publisher : Department of Crop Science, Faculty of Agriculture, Andalas University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jijcs.6.2.48-55.2024

Abstract

Weeds are plants whose presence is unwanted by humans because of competition with cultivated plants for nutrients, sunlight, and growing space, producing allelochemicals that interfere with plant growth, reduce production and product quality, and increase farming costs. The continuous use of synthetic herbicides has adverse effects on the environment and health, so it is necessary to seek a weed control mechanism that is more efficient and environmentally friendly. One is utilizing secondary metabolites, namely sunflower plant allelochemicals, that can control weeds. This study aims to determine the response of weeds to the application of extracts of sunflower plant parts as bioherbicides. This research was conducted in three stages: raw material preparation, extraction, and application. The results obtained 17 weed species in the experimental field of Nagari Selayo Tanang Bukit Sileh from 7 families (Asteraceae, Poaceae, Lythraceae, Polygonaceae, Brassicaceae, Caryophyllaceae, and Mazaceae) and two weed classes (broadleaf weeds and grasses). The bioherbicide efficacy of sunflower extract (Helianthus annus L.) has not caused symptoms of toxicity in weeds.
In silico analysis of Arbacia lixula-derived peptides and plasmid construction for recombinant anti-aging therapies Yenny, Satya W.; Jamsari, Jamsari; Hazmi, Auliya A.; Cuandra, Kevin N.; Hanifah, Wafiq; Yahono, Angela S.; Wahyudi, Dhyani P.; Buana, Gherriandi R.; Rahman, Awalil RK.; Maharani, Annisa D.; Firjatullah, Muhammad F.; Maulana, Rafi; Prayogi, Norbertus M.; Tristan, Christopher D.
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.1283

Abstract

Skin aging is one of the degenerative processes influenced by tyrosinase, elastase, collagenase, hyaluronidase, and matrix metalloproteinase-9 (MMP9) activity. One promising avenue for discovering antiaging therapeutics is the peptides from the Arbacia lixula spine. The aim of this study was to explore the potential of peptides from A. lixula spine as a multitarget inhibitor for recombinant antiaging therapies through in silico approaches. The crystal structure of peptides previously identified in A. lixula spine was visualized using the UCSF Chimera. The protein data bank (PDB) database was used to obtain the crystal structures of protein targets. The webservers Innovagen, AllerTop, and ToxinPred were utilized to predict the peptide's water solubility, toxicity, and allergenicity. MOE application was used to prepare all ligands and proteins, molecular docking, and visualization. Molecular dynamics simulations were carried out on the protein-ligand complexes on Yasara Dynamics application. The Benchling website was used to perform virtual electrophoresis and reconstruct the recombinant plasmid (Psb1c3). Based on the molecular docking results, peptide REGSPDLLE has the potential as a multitarget inhibitor of tyrosinase (-9.07 kcal/mol), hyaluronidase (-10.57 kcal/mol), elastase (-9.32 kcal/mol), collagenase (-10.57 kcal/mol), and MMP9 (-10.43 kcal/mol). Peptide REGSPDLLE was selected due to its strong binding affinity on the active site of each target protein and exhibits non-toxic, non-allergenic, and good water-soluble as indicated by Support Vector Machine score <0. Molecular dynamics simulations confirmed stable interactions with receptor proteins. Peptide REGSPDLLE was successfully inserted into the recombinant pSB1C3 plasmid, confirmed by virtual electrophoresis with bands at ~2000 bp and ~150 bp. Further in vitro and in vivo studies are necessary to verify the anti-aging efficacy of peptide REGSPDLLE.
Determination of Anthocyanin Content and Antioxidant Activity in Sidenuk Black Rice Mutant (Oryza sativa var. sidenuk) Respati, Aditya Nugraha; Sumaryati Syukur; Jamsari; Ishak
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 26 No. 01 (2025): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol26-iss01/580

Abstract

The Sidenuk black rice mutant is the latest development of Sidenuk Rice which is known to excel in high rice production and is resistant to pests. Due to the many health benefits offered by black rice and no data on anthocyanin content and antioxidant activity of the mutant black rice variety Sidenuk, further research is needed. This study aims to analyze the anthocyanin content and antioxidant activity of Sidenuk black rice mutants (Oryza sativa var. sidenuk). The yield of concentrated macerate of Sidenuk black rice mutant produced was 23.25%. Based on the results obtained in the qualitative test and information from the journal, it indicates that Sidenuk black rice mutant contains anthocyanin compounds, but not in Sidenuk white rice mutant. The cyanidin 3-glucoside compound was the most abundant anthocyanin compound in the Sidenuk black rice mutant, followed by peonidin-3-glucoside. The total anthocyanin content in black rice is 1.07 ± 0.04 mg/g. This data is higher than black glutinous rice but lower than other black rice varieties. The IC50 value of Sidenuk black rice mutant is 36.02±5.09 µg/mL, which is classified as strong antioxidant and is the strongest of all black rice varieties in this study.
PEMANFAATAN PLASTIK BEKAS SEBAGAI MEDIA TANAM RAMAH LINGKUNGAN DI KELURAHAN BUNGO PASANG KOTA PADANG Nefilinda Nefilinda; Jamsari Jamsari; Elvi Zuriyani; Rika Despica; Eryka Okta Juanty; Salma Salma
BULETIN ILMIAH NAGARI MEMBANGUN Vol. 3 No. 3 (2020)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bina.v3i3.242

Abstract

Bungo Pasang Village is one of the areas in Padang City. This area has densely populated settlements and houses with limited land. These settlements produce waste in the form of abundant organic or inorganic waste, which will impact environmental pollution. The activity objectives are to increase public knowledge and understanding of using used plastic bottles as a medium for planting flowers and environmentally friendly living pharmacies. Methods of activity: 1). lecture, 2). demonstration/training of using used plastic bottles as planting media. This activity's target audience is women in the Salingka Bungo Permai 1 Complex, Bungoasang Village, Koto Tangah District, Padang City, with about 30 members. Participants were also taught practice: punching plastic bottles, tying plastic bottles, mixing soil and fertilizer, planting flower plants in plastic bottles, and arranging plants vertically. The results of the activity obtained can increase public knowledge and awareness about using used plastic bottles as a planting medium. Furthermore, the community understands using used plastic bottles as an environmentally friendly planting medium in a little yard to the application stage of using used plastic bottles as an environmentally friendly planting medium. It is hoped that this activity can be carried out by the community in a sustainable manner to benefit people who have narrow yardlands. Furthermore, better management is needed to manage inorganic waste, such as using used bottles as planting media.
ISOLATION OF AnsB GENE FRAGMENT ENCODING L-ASPARAGINASE 2 ENZYME FROM Serratia plymuthica UBCF_13 AND IT’S IN-SILICO DOMAIN CHARACTERISTIC Ananda, Abi Awfa Rahman; Nursyafi, Fauzan Syarif; Aliya, Lisana Shidiqqin; Hasibuan, Imron Martua; Alioes, Yustini; Endrinaldi , Endrinaldi; Adrial, Adrial; Elmatris , Elmatris; Jamsari, Jamsari; Lily Syukriani
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2025): April 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/n5mfhy69

Abstract

Acute lymphoblastic leukemia(ALL) is one of the cancer diseases often occurs in children and causes high mortality in children. One of the chemotherapy treatment suggested is using L-Asparaginase 2. However due to its difficult production process making this approach expensive for the public. Therefore production technology of this enzyme is crucial enabling cheaper for ALL treatment. This study aimed to isolate the AnsB gene sequence from Serratia plymuthica UBCF_13 and perform its further in-silico analysis. The research was started by designing specific primers for the AnsB gene, isolating the AnsB gene fragment using PCR-based approach, sequencing the AnsB gene fragment, cloning the fragment to the plasmid vector and further transformed into E. coli DH5α cell. Further data analysis was carried out using some bioinformatics tools such as BLAST, MEGA X, I-TASSER InterPro. Sequence data result successfully verified that the full length of AnsB gene is 1047 bp. InterPro analysis indicated that the L-Asparaginase 2 from S. plymuthica UBCF_13 has 2 domains, namely L-Asparaginase N-terminal spanning from amino acid 26 to 216, while its C-terminal spanned from amino acid 235 to 345. The physical fragment of the gene was also successfully cloned to the pGEM-T Easy vector and subsequently transformed into E. coli DH5α cell. This result provided information for alternative sources of L-Asparaginase 2 and it’s possible engineering.
Lactococcus lactis D4 Has Potential Effect to Alleviate Inflammation and Reverse Dysbiosis in Colitis Rat Model Suswita, Rini; Alvarino, Alvarino; Darwin, Eryati; Jamsari, Jamsari
The Indonesian Biomedical Journal Vol 16, No 2 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i2.2970

Abstract

BACKGROUND: Inflammatory bowel disease (IBD) is a prevalent chronic inflammatory disorder of the gastrointestinal tract that may lead to colorectal cancer if untreated. Several medications for IBD have adverse side effects. Thus, there are high interest for searching alternative treatment to treat IBD. Lactococcus lactis D4 isolated from dadih, a traditional fermented buffalo milk product, was investigated for the effect on gut inflammation and microbial composition in the colitis rat model.METHODS: Eighteen male Sprague-Dawley rats were divided into three groups; control rats, colitis-induced rats, and colitis-induced rats treated with L. lactis D4 isolate. The control group received water, while the colitis-induced rats were given azoxymethane (AOM) and dextran sodium sulphate (DSS). Rats’ feces were collected for the analysis of gut microbiota diversity by next-generation sequencing and for the measurement of transforming growth factor (TGF)-β, nuclear factor kappa-B (NF-κB), interleukin (IL)-6, and tumor necrosis factor (TNF)-α colonic expression using reverse transcription-quantitative polymerase chain reaction (RT-qPCR).RESULTS: L. lactis D4 administration was able to reduce inflammation in colitis by decreasing IL-6 expression (0.87 vs. 0.73), while expression of TGF-β, NF-κB, and TNF-α were increased compared to the model group, suggesting a complex immunomodulatory effect. Gut microbiota sequencing revealed a similarity between the control and treatment groups, suggesting L. lactis D4 has great potential to ameliorate gut microbiota dysbiosis in colitis rats.CONCLUSION: L. lactis D4 has a beneficial effect in decreasing pro-inflammatory cytokines and is able to reserve dysbiosis in colitis rat model. Thus, L. lactis D4 might be used as a therapeutic agent for IBD.KEYWORDS: colitis, gut microbiota, inflammation, inflammatory bowel disease, Lactococcus lactis D4, probiotic, proinflammatory cytokines
Exploring the Therapeutic Landscape: A Systematic Review on the Anti-inflammatory Effects of Probiotics in Colitis-associated Colorectal Cancer Suswita, Rini; Alvarino; Eryati Darwin; Jamsari
Majalah Kedokteran Andalas Vol. 47 No. 3 (2024): MKA July 2024
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v47.i3.p341-361.2024

Abstract

Background: Colorectal cancer is closely linked to chronic inflammation; long-standing colitis confers a 2–3-fold higher risk. Modulating the gut–immune axis with probiotics may attenuate the inflammatory microenvironment of colitis-associated colorectal cancer (CAC). Objective:To evaluate the anti-inflammatory effects of probiotics in CAC animal models. Methods: We searched PubMed, ScienceDirect, and Wiley Online Library; 248 records were screened and 9 studies met inclusion. Risk of bias was appraised using SYRCLE RoB. Results: Probiotic treatment consistently reduced clinical severity, down-regulated inflammatory markers, and modulated key signaling pathways in CAC models. Nonetheless, evidence was heterogeneous, sample sizes were small, and dosing, strains, and formulations varied; safety reporting was limited. Conclusions: Probiotics show promise as anti-inflammatory adjuncts in CAC based on animal studies. Rigorous, standardized preclinical work and well-designed trials are needed to define optimal strains, dosing, and formulations, and to confirm efficacy and safety for translation.
Co-Authors -, Urnemi -, Vovien . Dachryanus Abdi Dharma Achmad Noerkhaerin Putra Adrial Adrial, Adrial Agustian Agustian Ahmad Denian Ali Rahmad Ali Rahmat Aliya, Lisana Shiddiqin Aliya, Lisana Shidiqqin Alvarino Alvarino Alvarino Ananda, Abi Awfa Rahman Andani Eka Putra Andika Ade Saputra Arif Sardi Armaini - Arnim Arnim Asman Manaf Azhar, Minda Azmi Dhalimi Buana, Gherriandi R. Cuandra, Kevin N. Dachryanus, Dachryanus Delmi Sulastri DESSY NATALIA Dian Pertiwi Djong Hon Tjong E. Y. Rahman E. Y. Rahman Efrida Efrida ekawati, fitri elara resigia Elly Syafriani Elmatris Sy ELVI ZURIYANI Elvi Zuriyani Endang Purwati RN Endrinaldi , Endrinaldi Eryati Darwin Eryati Darwin Eryka Okta Juanty Eryka Okta Juanty FA Febria Fatchiyah Fatchiyah Febiona, Keysha Putri Febria, FA Femy Riwany Firjatullah, Muhammad F. Friska Eka Fitria Gusmawartati Gusmawartati Hamda Fauza Hanifah, Wafiq Hasbi Al Huda Hasibuan, Imron Martua Hazmi, Auliya A. Herviyanti Herviyanti Ida Parwati Ifan Aulia Candra Irawati Chaniago Irfan Suliansyah ISHAK ISHAK MANTI Ishak Manti Ishak Manti Istino Ferita Jeanne Venora Julizar Julizar Jumsu Trisno Lidya Sari Utami Lily Syukriani Lipoeto NI Liza Aulia Yusfi Maharani, Annisa D. Maulana, Rafi Meisilva Erona Sitepu Minda Azhar Mirnawati Mirnawati ML Edy Parwanto Mochammad Imron Awalludin Muhamad Irsyad Murdaningsih H. Karmana N Nasir N Nurhidayat N Nurhidayat, N Nasril Nasir Nasril Nasir Nasrul Zubir Natalia, Dessy Nefilinda Nefilinda Nefilinda Nefilinda Netty Suharti Ningsih, Azma Risilvia Noraziyah Abd Aziz Shamsudin Nur Afrainin Syah Nurainas Nurainas Nursyafi, Fauzan Syarif Obel, Obel Prayogi, Norbertus M. Putra, Andika Chandra Rachmad Hersi Martinsyah Rahmad, Ali Rahman, Awalil RK. Rahmi Handayani Rahmi Kamelia Ramadhan, Nugraha Renfiyeni Reni Koja Respati, Aditya Nugraha Retno Ariza Soeprihatini Soemarwoto Rhenly Danis Rika Despica Rika Despica Rosadi, Firsta Ninda Roslaili Rasyid Roza Yunita Rusfidra rusfidra Sahrul Utama Saibi, Ihsan R. A. Saipul Sihotang Salma Salma Salma Salma Sanusi Ibrahim Sarbaini Sarbaini Sartika, Diza Selvia Dewi Pohan Seswita, Seswita Silvia Permata Sari Slamet Rianto SRI HENDRASTUTI HIDAYAT Sumaryati Syukur Sumaryati Syukur Suswita, Rini Syamsurizal Syamsurizal Syazili Mustofa Tika Runifah Trimuri Habazar TRIMURTI HABAZAR Tristan, Christopher D. Urnemi - Urnemi Urnemi V. Maslami Vovien - Wahyudi, Dhyani P. Wardi, Epi Supri Wawan Abdullah Setiawan Winda Purnama Sari Winda Purnama Sari Y Heryandi Y. Marlida Y. S. Nur Yahono, Angela S. Yanwirasti Yanwirasti Yanwirasti Yenny, Satya W. Yuniar Lestari Yurnalis Yurnalis Yustini Alioes ZK Abdurahman Baizal Zulkarnain Chaidir