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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner JURNAL ANATOMI FISIOLOGI Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Peternakan Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Bioma : Jurnal Biologi Indonesia Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) ARSHI Veterinary Letters FITOFARMAKA: Jurnal Ilmiah Farmasi EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup JFIOnline Jurnal Ilmu Kefarmasian Indonesia JCPS (Journal of Current Pharmaceutical Sciences) Jurnal Aisyah : Jurnal Ilmu Kesehatan Journal of Parasite Science Jurnal Kedokteran Hewan Jurnal Veteriner Nusantara
HERA MAHESHWARI
IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Nursing Public Health Veterinary Other

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Phase-dependent effects of purwoceng (Pimpinella alpina KDS) on estrous cycle modulation in virgin female rats Santoso, Hera; Achmadi, Pudji; Ekastuti, Damiana Rita; Tarigan, Ronald; Bustamam, Isdoni; Santoso, Koekoeh; Maheshwari, Hera; Satyaningtijas, Aryani
ARSHI Veterinary Letters Vol. 9 No. 4 (2025): ARSHI Veterinary Letters - November 2025
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.9.4.117-118

Abstract

Purwoceng (Pimpinella alpina KDS) merupakan tanaman obat tradisional yang telah banyak diteliti khasiat androgeniknya dalam penelitian reproduksi jantan. Namun, pengaruhnya terhadap kinerja reproduksi betina masih kurang dipahami. Penelitian ini bertujuan untuk menyelidiki pengaruh purwoceng terhadap durasi setiap fase estrus dan keseluruhan siklus estrus pada tikus betina. Empat puluh delapan tikus Sprague-Dawley betina perawan dibagi menjadi empat kelompok berdasarkan fase estrus mereka pada awal perlakuan: proestrus, oestrus, metestrus, dan diestrus. Setiap kelompok terdiri dari enam ekor kontrol dan enam ekor tikus yang diberi Purwoceng. Ekstrak diberikan secara intragastrik selama sepuluh hari berturut-turut, sementara evaluasi apusan vagina dilanjutkan hingga hari ke-15 untuk memantau siklus estrus. Hasil penelitian menunjukkan bahwa pemberian purwoceng selama dua siklus estrus umumnya memperpanjang fase proestrus dan estrus, dengan perpanjangan fase estrus yang signifikan secara statistik diamati ketika perlakuan diberikan selama estrus (P < 0,05). Sebaliknya, durasi fase metestrus dan diestrus berkurang, kecuali ketika Purwoceng diberikan selama metestrus, yang mengakibatkan peningkatan durasi metestrus. Kesimpulannya, purwoceng menunjukkan efek modulasi terbesar ketika diberikan selama fase estrus, menunjukkan responsivitas siklus reproduksi betina yang bergantung pada fase terhadap ekstrak herbal ini.
Prevalence of mcr-1 Colistin Resistant Gene in Escherichia coli Along the Broiler Meat Supply Chain in Indonesia Palupi, Maria Fatima; Wibawan, I Wayan Teguh; Sudarnika, Etih; Maheshwari, Hera; Darusman, Huda Shalahudin
BIOTROPIA Vol. 26 No. 2 (2019): BIOTROPIA Vol. 26 No. 2 August 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2019.26.2.1054

Abstract

Colistin is the last drug of choice for dealing with carbapenem-resistant Enterobacteriaceae bacteria; hence, this drug is very crucial to human health. The discovery of a plasmid-mediated colistin-resistant gene, the mobilized colistin resistance-1 (mcr-1), signals a significant global health threat. Colistin sulfate is an antimicrobial agent which has been approved for use in broilers in Indonesia. Thus, this study aimed to measure the prevalence of colistin-resistant Escherichia coli and to detect the mcr-1 colistin-resistant gene in E. coli and E. coli O157:H7 in the entire supply chain of broilers in Bogor Regency, West Java Province, Indonesia. Samples were taken from 47 flocks that used colistin sulfate (47 pools of cloacal swabs, 47 pools of drinking water, and 47 pools of litter), seventy fresh meat samples and seven plucker swab samples from seven small-scale poultry slaughterhouses, seventy fresh meat samples from seven traditional markets, and seventy cooked meat samples from seven small restaurants. The isolation of E. coli was done on each of the 358 samples, and 493 isolates were obtained. All the E. coli isolates were then tested for their susceptibility to colistin sulfate by using the agar dilution method. The detection of the mcr-1 gene from the colistin-resistant isolates (minimum inhibitory concentration > 2 µg/mL) was conducted using the polymerase chain reaction (PCR). The prevalence value of colistin-resistant E. coli in all the isolates was 11.76% (CI 95%; CL 9.21–14.91), and the prevalence of the mcr-1 gene was 10.55% (CI 95%; CL 8.13–13.57%). A very good agreement correlation existed between the colistin-resistant phenotype and the mcr-1 gene (κ = 0.939). The mcr-1 gene was found in 89.66% of colistin-resistant E. coli isolates. Two colistin-resistant and mcr-1 carrying isolates were identified as E. coli O157:H7 serotype. This research was the first study attempt on the mcr-1 gene in Indonesia, covering the entire supply chain of broiler meat from farms to consumers. The results indicated the necessity to reduce the use of colistin sulfate in broiler management and to improve biosecurity measures, not only in farms but also in the entire supply chain of broiler meat production.
Co-Authors . Herdis . Safrida . Yulnawati A. Suprayogi A. Wijaya A.S. Satyaningtijas ACHMAD FARAJALLAH Agik Suprayogi Agus Wijaya Ainul Khadija Saleema Akhiruddin Maddu Amelia Hana Amelia Ramadhani Anshar Anak Agung Gede Sugianthara Anak Agung Istri Sri Wiadnyani Andhika Yudha Prawira Andre, Daniel Latief Andriyanto Andriyanto Arief Boediono Asmarani Kusumawati Bambang Kiranadi Bambang Kiranadi Bambang Kiranadi Bambang Purwantara Claude Mona Airin D. R. Ekastuti Damhuri Damhuri Damiana Rita Ekastuti Danang Dwi Cahyadi David Anwar Dawanto, Jirfan Debby Jacqualine Jochebed Rayer Desrayni Hanadhita Desrayni Hanadhita Desrayni Hanadhita Diah Nugrahani Pristihadi Diani, Yusias Hikmat Dirwan Rahman Divo Jondriatno Divo Jondriatno Dondin Sajuthi Dwi Nurhidayah Zubaidah E Mulyati Effendi, E Mulyati Ekowati Handharyani Elok Budi Retnani ERIC HAYES Ernes Andhesfa Etih Sudarnika Eva Harlina Evi Juliati Evi Juliati Gani F. B. Harlimawan Faisal Tanjung Fitri, Arni Diana Fredi Kurniawan Gandasari, Ira Agustina Dewi Ganjar Maulana Nugraha Ganjar Maulana Nugraha Gilang Kala Maulana, Gilang Kala Hadi S Alikodra Hardiansyah, Muhamad Evans Hendry, Altaff heny Nitbani Herdis - Huda Salahuddin Darusman Huda Salahudin Darusman Huda Shalahudin Darusman Husna Husna, Mar’atul Husna, Rina Siti Nurul I wayan Teguh Wibawan ikhwan wirahadikesuma Ikhwan Wirahadikesuma Irfan Nurhidayat, Irfan Irwan Oktoni Isdoni Bustaman Jefri Naldi Julianto . Juliati Gani, Evi Juliati, Evi Jumadin, La Kasiyati Kasiati koekoeh santoso Kusdiantoro Mohamad Kusuma, Rini Anjarwati La Jumadin Lili Darlian Listya M.I, Mega LUTHFIRALDA SJAHFIRDI Luthfiralda Sjahfirji Luthfiralda Syahfirdi Maneewong, Sattabongkoch Mar’atul Husna Maria Fatima Palupi Maria Fatima Palupi Maria Fatima Palupi Maria Fatima Palupi Maria Prihtamala Omega Maryce Agusthinus Walukou Mega Listya M.I Meta Levi Kurnia Mirnawati Bachrum Sudarwanto Mokhamad Fakhrul Ulum, Mokhamad Mozes R. Tolihere MUHAMMAD ADIB MUSTOFA Muhammad Darjat Darulfalah Muhammad Darjat Darulfalah Muhammad Gunawan Muhammad Rizal Muhammad Zhaahir Mulyati Effendi MULYATI EFFENDI Nambut, Yohanes Baptisto Nastiti Kusomorini Nastiti Kusomorini Nastiti Kusumorini NASTITI KUSUMORINI NASTITI KUSUMORINI Niken Ulupi Nikki Aldi Nikki Aldi, Nikki Nugroho, Setyo Widi Nuri Ardiani Ong Huey, Lynette P. Achmadi Perdhana, Ika Satya Permana, Nadine Hanifa Pudji Achmadi Pudji Astuti Pudji Astuti PUDJI ASTUTI Pudji Astuti Pudji Astuti Purnomo, Rachmatullah Hadi Putri Indah Ningtias Putri Krida Gita P. Putri Krida Gita P. Putri, Fatimatus Sa’diyah Putri, Maritrana R. Tarigan Rachmatullah Hadi Purnomo Rahma Anisa Rahman, Muhammad Luthfi Reviany Widjajakusuma Reviany Widjajakusuma Reviany Widjajakusuma Ridi Arif Rina Siti Nurul Husna Risa Tiuria Rivany Widjajakusuma Rosdianto, Aziiz Mardanarian Rosdianto, Aziiz Mardanarian Rudramurti, Win Satya Safrida Safrida Sandra Hapsari Sandra Hapsari Santoso, Hera Sarmin - Sasmita, Annissa Nuridfi Satvika, Fadhila Siagian, Forman Erwin Slamet Widiyanto Srihadi Agungpriyono Sumiaty Aiba Supratikno . Tarigan, Ronald Teguh Budipitojo Trini Suryowati, Trini Tuty Laswardi Yusuf Umi Cahyaningsih Utami, Dian Maulia W. Manalu Wasmen Manalu Wisnugroho Agung Pribadi Wisnugroho Agung Pribadi Yan, Teo Qin YENI KEZIA BEKALANI Yulnawati - Yulvian Sani Yusfiati Yusfiati Zora, Nelda Fliza