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All Journal BALI MEDICAL JOURNAL (BMJ) journal of internal medicine International Journal of Biosciences and Biotechnology Jurnal Ilmu dan Kesehatan Hewan Buletin Udayana Mengabdi COPING (Community of Publishing in Nursing) INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Veteriner Jurnal Biologi Udayana Microbiology Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Media Kedokteran Hewan Molecular and Cellular Biomedical Sciences (MCBS) ISM (Intisari Sains Medis) : Jurnal Kedokteran Journal of Global Pharma Technology Jurnal Medika Veterinaria Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
I NYOMAN MANTIK ASTAWA
Laboratorium Virologi, Departemen Penyakit Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali-Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Journal : INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES

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CENTELLA ASIATICA EXTRACT INCREASED ON THE LEVEL OF INTERLEUKIN 6 (IL-6) IN MICE Kerta Besung, I Nengah; Mantik Astawa, Nyoman; Suatha, I Ketut; -, Hartaningsih
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 5, No. 1 Januari 2011
Publisher : Udayana University

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Abstract

Salmonellosis is still problem in many developing countries including Indonesia. Themain problem in controlling and handling the disease is that only few antibiotics are availableto cure the disease. In addition, the prolonged use of such antibiotics often lead to bacterialresistant against the antibiotics. A herbal drugs such as Centella asiatica (in Indonesia isknown as pegagan) contains triterphenoid saphonins which acts as immunostimulant capableof enhancing the phagocytic activity of macrophages. However, no study has been conductedto investigate the use pegagan in activating macrophage of mice infected with Salmonellatyphi. A study was therefore conducted to find out the ability of Cantella asiatica inenhancing on the level interluekine (IL)-6 following challenge with Salmonella typhi. It istherefore expected that herbal drug such as Cantella asiatica can be used as an alternativemedicine to prevent and cure salmonellosis in both animals and human.Experimental laboratory studies were conducted using Completely FactorialRandomized Design. Mice were divided into 4 groups and they were treated respectively withdestilated water (negative control), 125, 250, and 500 mg/kg bw of Centella asiatica extract.The treatment was conducted daily for 2 weeks and the mice were then inoculated with 105cells of S. typhi. The level of IL-6 response were examined 24 hours, 2 weeks, and 4 weeksafter inoculation with S. typhi.The result showed that treatment of mice with Centella asiatica extract significantly(p<0,05) enhaced IL-6 level of Balb/c mice following inoculation with S. typhi. The highestIL-6 level were observed in mice treated Centella asiatica extract at the dose of 500 mg/kgBW (385,9257±125,4314 pg/ml serum). And the highest IL-6 level were observed at 2 weeksfollowing inoculation with S. typhi (533.4262 ± 81.7184 pg / ml).A further study is recomended to examine the celluler immune response and moredetailed study on the humoral immune response of animals or human before this herbal isused as alternatif medicine to prevent and cure typhoid fever. It is also important to study thebest preparation, the half life, and the side effect of Centella asiatica in human and animals.
RESPON KEKEBALAN HUMORAL MENCIT BALB/C YANG DIVAKSINASI DENGAN VAKSIN LIMPA DAN VAKSIN KULTUR PENYAKIT JEMBRANA TERHADAP PROTEIN VIRUS JEMBRANA MANIK WIDIYANTI, NI LUH PUTU; SUATA, I KETUT; MANTIK ASTAWA, I NYOMAN; -, HARTANINGSIH
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 2, No. 2 Mei 2008
Publisher : Udayana University

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Abstract

Sapi bali adalah salah satu aset nasional Indonesia yang harus dilestarikan karena mempunyai keuntungan ekonomi. Tetapi sapi bali mempunyai beberapa kelemahan penyakit khususnya penyakit Jembrana yang disebabkan oleh virus penyakit Jembrana (JDV). Pencegahan terhadap penyakit Jembrana telah dilakukan dengan vaksinasi. Vaksin yang terbukti dapat menurunkan tingkat kematian sapi bali terserang JDV adalah vaksin limpa. Jenis vaksin ini hanya mampu menginduksi kekebalan dengan perlindungan 70%. Proteksi ini dapat ditingkatkan jika jumlah virus yang digunakan dalam vaksin meningkat. Tekhnik kultur in vitro adalah salah satu metode meningkatkan jumlah virus penyakit Jembrana, dan selanjutnya dibuat vaksin kultur. Hasil penelitian menunjukkan bahwa sel limfosit sapi bali terinfeksi JDV adalah 9,5% pada limpa dan 57,43% pada sel kultur. Uji westernimmunoblotting sel limfosit sapi bali dari darah tepi dan limpa terinfeksi JDV menggunakan antibodi monoklonal (AbMo) anti Ca, terdeteksi protein dengan berat molekul 26 kDa, 42 kDa dan 51 kDa. Pada medium kultur PBMC dan endapan plasma sapi bali terinfeksi JDV, teridentifikasi protein dengan berat molekul 16 kDa an 26 kDa menggunakan AbMo, dan teridentifikasi protein dengan berat molekul 16 kDa; 21,5 kDa. 26 kDa; 29,7 kDa; 40 kDa dan 50 kDa menggunakan AbPo. Uji Elisa didapatkan nilai absorban antibodi mencit balb/c yang divaksinasi dengan vaksin kultur penyakit Jembrana lebih tinggi yaitu sebesar 0,3089 dibandingkan vaksin limpa yaitu sebesar 0,177 dengan p<0,05. Nilai absorban antibodi mencit balb/c terhadap antigen Ca, SU dan tat, memperlihatkan nilai absorban terhadap antigen SU berbeda sangat signifikan dibandingkan dengan antigen Ca dan antigen tat (p<0,01). Antigen Ca berbeda signifikan terhadap antigen tat (p<0,05).
HUBUNGAN POSITIF ANTARA ULKUS KAKI DIABETIK DENGAN PERSENTASE SEL BERMARKAH CD4+ PEMBAWA MALONDIALDEHID Putu SutirtaYasa, I Wayan; Suastika, Ketut; Sudewa Djelantik, Anak AgungGede; Mantik Astawa, I Nyoman
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 3, No. 1 Januari 2009
Publisher : Udayana University

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Abstract

Tingginya angka kejadian ulkus kaki diabetik (UKD) dan luka di kaki yangsulit sembuh memberi petunjuk kemungkinan ada proses kematian sel imun yangsangat banyak dan belum jelas mekanismenya secara molekuler pada jaringan UKD.Telah diteliti hubungan antara derajat UKD dengan persentase sel bermarkahCD4+ pembawa malondialdehid (MDA). Penelitian ini adalah penelitianobservasional dengan rancangan cross sectional analytic study yang dilakukan diRumah Sakit pemerintah dan swasta di Denpasar, Badung, Tabanan, dan Gianyar.Parameter yang diukur dari bahan darah adalah kadar gula darah memakai metodeenzimatik (heksokinase), dan dari bahan jaringan kaki, dihitung sel bermarkah CD4+pembawa MDA memakai metode imunohistokimia (reagen dari Biodesign danAbcam ). Dari 80 sampel UKD didapatkan 49 (61,2%) penderita laki-laki dan 31(38.8%) penderita wanita, berdasarkan tingkat keparahan UKD, sampel dipilah lagimenjadi: 29 (31,9%) derajat 2; 20 (21,9%) derajat 3; 13 (14,3%) derjat 4; dan18(19,8%) derajat 5, rata-rata persentase sel bermarkah CD4+MDA adalah 75,0 ±20,5 %, Didapatkan korelasi positif kuat antara persentase sel bermarkah CD4+pembawa malondialdehid dengan derajat UKD (r = 0,71; p < 0,01). Pada penelitianini membuktikan ada mekanisme kematian sel imun dan sekaligus menjawabpermasalahan bahwa pada penderita UKD mudah terkena infeksi dan sulit untukdisembuhkan, dengan dibuktikan bahwa ada korelasi positif kuat antara derajat UKDdengan persentase pembentukan MDA dari sel bermarkah CD4+, ini menyatakanbahwa semakin berat derajat UKD semakin banyak mengalami kematian sel imun..
ANTIOXIDANT CAPACITY OF FRANGIPANI (Plumeria alba) POWDER EXTRACT Wrasiati, Luh Putu; Wirawan, I Gede Putu; Agus Bagiada, Nyoman; Mantik Astawa, I Nyoman
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 5, No. 2 Mei 2011
Publisher : Udayana University

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Abstract

This research aimed to identify the antioxidant capacity, vitamin C (ascorbic acid), and total phenolic compounds of frangipani flower powder. The powder was extracted using ethanol, methanol, acetic acid, and water (aquadest). Antioxidant capacity of each extract were determined using 2,2-diphenyl-1-picrylhydrazyl radical scavenging method, vitamin C were determined using 2,4 Dinitrophenylhydrazine, and total phenolic compounds were determined using Folin Ciocalteu reagent. All of parameters were measured by spectrofotometer. The result shows that the highest value of antioxidant capacity was ethanolic extract (18.19%) and the lowest value was acetic acid extract (12.74%). The highest value of vitamin C was aqueous extract (3.49 mg/100g) and the lowest value was acetic acid extract (3.02 mg/100g). The highest value of total phenolic content was aqueous extract (25.49 mg GAE/g) and the lowest value was acetic acid extract (22.74 mg GAE/g). In conclusion, the higher antioxidant capacity was not always followed by the higher of vitamin C and total phenolic compounds.
ALDEHYDE DEHIDROGENASE LEVEL AND FATTY ACID ETHYL ESTER AS BIOCHEMICAL MARKERS PERSIST LONGER THAN ETHANOL IN WISTAR RATS AFTER CHRONIC ALCOHOL CONSUMPTION Suaniti, Ni Made; Sudewa Djelantik, A. A. Gde; Suastika, Ketut; Mantik Astawa, Nyoman
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 4, No. 2 Juli 2010
Publisher : Udayana University

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Abstract

Alcohol consumption in human has increased from year to year in Indonesia and more recently, anincreasing number of cases of alcohol intoxication, alcoholic liver disease, and death were observed.The purpose of this experimental study was to examine the significance of two known biochemicalmarkers of alcohol given by mouth in the Wistar rats. The study design used was the “Truerandomized experimental post test only control group design". The rats were randomly distributedaccording to the experimental design and were treated daily for six weeks (chronic intake) with 5%and 20% alcohol. This study used 15 rats with 5 rats for treatment group treated with 5% alcohol, 5rats for treatment group treated with 20% alcohol, and 5 rats as control group treated with distilledwater. The biochemical markers were aldehyde dehydrogenase (ALDH) and Fatty Acid Ethyl Esters(FAEE). ALDH and FAEE were two biochemical markers of ethanol which are sensitive and specificfor alcohol consumption. The study was conducted in two phases. Initially, rats were treated orallyeveryday for six weeks with 5% and 20% alcohol, and then the blood level of ethanol, ALDH andFAEE were measured. Blood samples were collected at 6 and 24 hours after the last oral intake ofchronic alcohol administration. Qualitative analysis was carried out to detect the presence of ethanol,ALDH, and FAEE in the treatment groups and quantitative analysis to determine their levels in theblood of Wistar rats. Statistical analysis of ALDH was done by using parametric test and the presenceof FAEE persisting longer than ethanol by non-parametric test. The results showed that ALDHpersisted and increased significantly following chronic consumption of alcohol in the rats. Similarly,FAEEs persisted longer than ethanol after alcohol intake. After six hours, the ALDH level increasedby 108.14% in the rat treated chronically with 5% alcohol and by 85.07% in rat treated with 20%alcohol. After 24 hours, FAEE also persisted longer in the blood than ethanol following treatmentwith alcohol 5%. ALDH levels increased by 83.11% after chronic treatment with 5% alcohol and by112.05% in the rats treated with 20% alcohol. In the blood collected 24 hours after the last treatmentwith 5% alcohol, ALDH increased by 95.11% and by 86.79% in the rats treated with 20% alcohol.FAEE persisted longer than ethanol in the blood following administration of 5 % and 20% alcoholboth at 24 hours following chronic treatment. The longer persisting ALDH and FAEE were new andgood biochemical blood markers for chronic alcohol consumption in the Wistar rats.
THE HIGH PLASMA RETINOL BINDING PROTEIN 4 LEVEL AS A RISK FACTOR CONSEQUENTLY OF TYPE 2 DIABETES MELLITUS OF ABDOMINAL OBESITY Budhitresna, AAG; Suastika, Ketut; Mantik Astawa, Nyoman; Santoso, Anwar
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 5, No. 1 Januari 2011
Publisher : Udayana University

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Abstract

Abdominal obesity (Ab-Ob) related to cardiometabolic risk, that is riskfactor constellation for succeeded cardiovasculer disease and type 2 DiabetesMellitus (DM). That factors such as atherogenic dislipidemia, hypertension,hyperglycemia, protrombotic state, and proinflammation state. Type 2 DMcharacterised by insulin resistance (IR). Plasma levels of retinol binding protein 4(RBP4) that is secreted by adipocytes are increased in insulin resistance (IR) state.Experiment in mice suggest that elevated RBP4 level cause IR. Although theunderlying mechanism is not clearly understood, RBP4 considered playimportance role consequently of type 2 DM in Ab-Ob.This research was carried out to determine the role of high plasma RBP4level as a risk factor consequently of type 2 DM in Ab-Ob. The research wasconducted by cross sectional analytic in 81 patients with Ab-Ob and case controlstudy with matching on 33 patients with Ab-Ob type 2 DM as cases and 33patients with Ab-Ob non type 2 DM as control. The plasma of TNF-?, sTNFR1,and RBP4 levels was measured by ELISA. IR status of the patients wasdetermined by HOMA-IR, whereas the ?-cell function was determined byHOMA-B. Ab-Ob was defined by using criteria for Asian peoples (male WC ? 90cm; female WC ? 80 cm). The result of 81 patients with Ab-Ob showed that bothplasma of TNF-? and sTNFR1 levels were significant positive correlated withplasma RBP4 level (coeficient correlation r = 0,294; p = 0,008 dan r = 0,458; p =<0,001 respectively). In addition, the plasma of RBP4 level significantly positivecorrelation with HOMA-IR (r = 0,450; p = 0,000) and significantly negativecorrelation with HOMA-B (r = -0,564; p = <0,001). In the matched case-controlstudy, it was shown that mean plasma of RBP4 level of type 2 DM group (76,08 ±16,84 ?g/ml) statistically higher than that without type 2 DM group (41,13 ±14,75 ?g/ml) (p = <0,001). The odds ratio higher plasma of RBP4 level was 5,426(CI 95%; 1,343 – 21, 928) statistically significant for increases risk type 2 DM (p= < 0,05). It has been proven that RBP4 was a dominant and consisten risk factor(66.9%, p = < 0.001) which influenced the incidence of type 2 DM in Ab-Ob. It can be concluded that high plasma of RBP4 level have a greater risk tosuffered from type 2 DM compared to low plasma of RBP4 in Ab-Ob. The highplasma of RBP4 level is most dominant and consistent risk factor consequently oftype 2 DM. These mechanism could behind the association between high plasmaof RBP4 level and type 2 DM.
INHIBITION OF BIFIDOBACTERIUM sp ISOLATED FROM INFANTS FECES TOWARDS ADHESION OF SALMONELLA TYPHI ON BALB/c MICE ENTEROCYTE Sukrama, I D. M.; Sukardika, K.; -, Sumarno; Mantik Astawa, N.
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 4, No. 1 Januari 2010
Publisher : Udayana University

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Abstract

Diarrhea, up to the recent year remains a cause of high morbidity and mortalityworldwide, especially in developing countries including Indonesia. Research concerning ofmanagement, prevention, and medication of the disease have been continually improved. Theaim of this research is searching Bifidobacterium sp isolated from infants feces. ThisBifidobacterium was then applied as an anti-adhesion of Salmonella typhi in the hope to gain acure of diarrhea. This research employed two research designs, namely descriptive explorationand true experimental. Exploration was applied in order to obtain isolation and characterizationof Bifidobacterium isolated from infants feces. Adherence ability of this Bifidobacterium sptowards Salmonella typhi adhesion on mice entherocyte was then carried out by applyingRandomized Posttest-Only Control Group Design. In this research, average Bifidobacterium spadhesion index of 1950 on entherocyte was obtained. In simple word, there are 19.5Bifidobacteria adhere to any single entherocyte cell. This adhesion index value is highercompare to Salmonella typhi adhesion of 1504. Conclusions that can be drawn from this researchare the finding of Bifidobacterium sp isolated from infants feces. This Bifidobacterium sp has anability to inhibit adhesion of Salmonella typhi on BALB/c mice enterocyte. Future work that canbe carried out are further researches concerning whether these bacteria have an ability to inhibitadherence of other pathogen bacteria. More over, searching of cell wall adhesin ofBifidobacterium sp that can be used as a replacement of life probiotic bacteria is also a greatinterest of research to be carried out.
THE HUMORAL IMMUNITY RESPONSE OF DOG VACCINATED WITH ORAL SAG2 AND PARENTERAL RABISIN AND RABIVET SUPRA92 -, Faizah; Mantik-Astawa, IN; Putra, AAG; -, Suwarno
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 6, No. 1 Januari 2012
Publisher : Udayana University

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Abstract

ABSTRACT This research aims to determine whether SAG2 oral vaccine induced antibody response as high as that of parenteral vaccine (Rabisin and Rabivet Supra 92). The experimental design applied was randomized pre and post control group design with 4 treatments (oral SAG2 vaccine, parenteral rabisin, parenteral Rabivet Supra 92 and control without vaccination) and with 9 replication. As many as 36 kampung dogs used and the anti rabies antibody was determined by enzyme-linked immunosorbent assay (ELISA) at day 0, 21, 56, 84, 119, and 147 post vaccination. The antibody titer induced by oral SAG2 vaccine raised more slowly but persisted longer than parenteral vaccine. On day 21 after vaccination, the average antibody titers induced by oral SAG2 vaccines (0.501 IU/ml) was still significantly lower than those induced by parenteral Rabisin (3.504 IU/ml) and Rabivet Supra92 (2.545 IU/ml). However, by day 119 after vaccination the average antibody titer induced by SAG2 oral vaccine (0.889 IU/ml) was significantly higher (p <0.05) as compared to those of parenteral Rabisin (0.625 IU/ml) and Rabivet Supra 92 (0.223 IU/ml). The antibody response induced by SAG2 oral vaccine appears to persist longer than parenteral vaccines and it can therefore be used as an alternative vaccine to combat rabies in animals, especially for dogs kept in free range.
ORAL INTAKE OF SARDINELLA LONGICEPS OIL THE DECREASE OF TNF-? AND IL-6 LEVELS IN ATHEROSCLEROTIC WISTAR RAT Wahjuni, S.; Sudewa, A.A.G.; Wita, I Wayan; Astawa, I.N. Mantik
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 5, No. 2 Mei 2011
Publisher : Udayana University

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Lifestyle changes to consumption of variegated instant food may be associated several heath hazards, such as obesity, dyslipidemia, and atheroschlerosis. This study was conducted to investigate the effects of orally administered Sardinella longiceps oil as an anti inflammatory agent on the serum levels of TNF-? and IL-6 considered as biomarkers for atherosclerosis.The study design is an true experimental with randomized pretest and posttest control group design, using 50 Wistar rat equaly divided into 5 groups, i.e. placebo control group 0% and 4 treatment groups each treated daily with 10%, 15 %, 20 % and 25 % fish oil respectively for 6 weeks. Before the treatment was started, all rats were orally fed daily with a high cholesterol diet for 13 weeks to induce atherosclerosis. Our study showed that the intake of 20% fish oil had resulted in the significantly greatest decrease of 45,63 % in the TNF-? serum levels,from 28.62 ± 1.25 to 15.56 ± 7.20 ?g/mL and similar significant decrease 15,42% in of IL-6 serum levels from 134.64 ± 1.98 to 113.87 ± 4.30 ?g/mL. The overall results of our study seemed to imply than in the Wistar rats, oral intake of Sardfinella longiceps oils signifacantly decreased serum levels of TNF-? and IL-6 probably through their anti-inflamatory effects. Futher research to determin the magnitude of effects sardinella longiceps oils on the serum levels TNF-? and IL-6 human.
MASSAGE STIMULATION REDUCES TUMOR NECROTIC FACTOR-ALPHA AND INTERLEUKIN-6 IN PRETERM, LOW BIRTH WEIGHT WITH APPROPRIATE GESTATIONAL AGE NEWBORNS Trisna Windiani, I G. A.; Sotjiningsih, S.; Mantik Astawa, N.; Kusnandi, and R.
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol 7 No 2 (2013): IJBS Vol2 No2
Publisher : Udayana University

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Abstract

Preterm newborn is susceptible from various stresses such as infections or non infections. During stress, immune response is activated by synthesizing and releasing of cytokines from activated immune cells into the circulation. One of the efforts to overcome the stress is massage stimulation. Several studies have been carried out to find out the benefit of massage stimulation. The objective of this study is to find out the effect of massage stimulation to reduce of TNF-? and IL-6 levels on preterm, low birth weight, appropriate to gestational age newborn. This was a randomized controlled trial. Subjects of the study were all preterm newborns with a stable medical condition, birth weight 1,500-2,499 gram, appropriate to gestational age. The study was carried out on October-December 2011, in neonatology ward, Sanglah General Hospital, Vali-Indonesia. The number of cases was 35 subjects and the number of controls was 36 subjects. The examination of TNF-?, IL-6 levels and birth weights before and after massage stimulation were performed on both groups. The level of TNF-? and IL-6 was examined by applying ELISA method. The level of confidence was ? = 0.05. All statistical analysis was performed by computer programme. There was different level of cytokine pro-inflammation TNF-? (p=0.025) and IL-6 (p=0,001) comparing before and after condition massage stimulation. Outcome of body weight were also different at before and after massage stimulation, with p value 0.042. The conclusion of this study is that massage stimulation can reduce the level of TNF-?,   IL-6 and increasing of birth weight on preterm, low birth weight appropriate gestational age newborn. Based on this study, in the future, massage stimulation can be done in newborn with stable medical conditions by parents or trained staffs.
Co-Authors A. A. G. P. Wiraguna A.A. Wiradewi Lestari A.A.G. Sudewa AAG Budhitresna AAG Putra Ahmad Hamim Sadewa Aida Lousie Tenden Rompis Alberto Agustinho Pereira Da Costa Joao Anak Agung Ayu Mirah Adi Anak Agung Bagus Bramardipa Anak Agung Gede Sudewa Djelantik Anak Agung Keswari Krisnandika Anak Agung Ngurah Subawa Anak Agung Oka Wijaya Anak Agung Sagung Kendran and R. Kusnandi Andika Budi Kurnianto Anwar Santoso Arthawan Arthawan Bayu Setiabudi Berata , I Ketut Chandra Yowani Dewa Ayu Agus Sri Laksmi DWI SURYANTO Dyah Kanya Wati Faiziah - G.A.M.K. Dewi Gusti Ayu Mayani Kristina Dewi Gusti Ayu Yuniati Kencana Gusti Ngurah Narendra Putra Harjana, Ngakan Putu Anom HARTANINGSIH - Hartaningsih . I D. N. Wibawa I Dewa Made Sukrama I Gede Ngurah Harry Wijaya Surya I GEDE PUTU WIRAWAN I Gusti Agung Arta Putra I Gusti Agung Ayu Suartini I Gusti Agung Dewi Sarihati I Gusti Agung Trisna Windiani I Gusti Ayu Putu Eka Pratiwi I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Gusti Ngurah Sudisma I K. Sukardika I Kadek Swastika I Ketut Berata I Ketut Eli Supartika I Ketut Junitha I Ketut Suada I Ketut Suada I Ketut Suastika I Ketut Suastika I KETUT SUATA I Ketut Suatha I Ketut Suwiyoga I Made Bakta I Made Damriyasa I Made Dwinata I Made Galih Diparayoga I Made Jawi I Made Kardena I Made Subrata I Made Sudarmaja I Made Sukada i Nengah Wandia I Nyoman Agus Bagiada I Nyoman Budi Hartawan I Nyoman Polos I Nyoman Suarsana I Nyoman Suartha I Putu Sudiarta I W. Wita, I W. I Wayan Bebas I Wayan Gorda I Wayan Masa Tenaya I Wayan Megadhana I Wayan Putu Sutirta Yasa I Wayan Suardana I Wayan Wita I.A.P. Apsari I.B.K. Suardana I.H. Utama I.W. Batan Ida Ayu Pasti Apsari Ida Bagus Gede Suparyatha Ida Bagus Kade Suardana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Suardana Ida Bagus Subanada Ignatius Ferdi Yuatmadja Inna Narayani K. Sri Marhaeni Julyasih K. Suata K. Sukardika Kadek Karang Agustina Ketut Budiasa Ketut Santhia Adhy Putra Ketut Suata Ketut Tirtayasa Luh Dewi Anggreni Luh Putu Agustini LUH PUTU AGUSTINI Luh Putu Wrasiati M.D. Rudyanto M.Pd S.T. S.Pd. I Gde Wawan Sudatha . Made Damriyasa, Made Made Oka Ari kamayani, Made Oka Ari Made Wiryana Marissa Divia Dayanti Marson, Fransiska Gratia Sonita Mudinillah, Adam N. T. Suryadhi Ngakan Putu Anom Harjana Ni Luh Putu Eka Diarthini NI LUH PUTU MANIK WIDIYANTI Ni Made Krisna Dewi Ni Made Suaniti NINING HARTANINGSIH Nyoman Agus Bagiada Nyoman Tigeh Suryadi Oka Lely Palagan Senopati Sewoyo Purwitasari, Made Santi Putu Ayu Asri Damayanti Putu Ayu Sisyawati Putriningsih Rasmaya Niruri Rasmaya Niruri S. Soetjiningsih, S. S. Sotjiningsih S.K. Widyastuti sang gede purnama Sewoyo, Palagan Senopati Siti Maryam Soetjiningsih - Soetjiningsih . Sri Kayati Widyastuti Sri Wahjuni SUMARNO Suryadi, Nyoman Tigeh Sutjahjo Suherman, Sutjahjo Suwarno - T. Sari Nindia Tjok Gede Oka Pemayun, Tjok Gede Oka Tjokorda Gde Agung Suwardewa Wayan Tunas Artama Wimpie I Pangkahila Yasunobu Matsumoto Yosevangelika Hutabarat Yoshihiro Hayashi