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Physiological Response of Cantang Grouper as Zeylanicobdella Control with Papaya Leaf Extract as an Antiparasitic Agent Ikmalia Amali; Gunanti Mahasri
Jurnal Biosains Pascasarjana Vol. 24 No. 2 (2022): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i2.2022.66-73

Abstract

Permintaan pasar terhadap ikan kerapu cantang yang terus meningkat menyebabkan intensifikasi budidaya tidak dapat dihindarkan. Salah satu kendala utama dalam upaya peningkatan produksi ikan kerapu adalah serangan penyakit. Alternatif bahan obat yang dapat digunakan dalam pengendalian penyakit yaitu dengan menggunakan daun pepaya lokal dan daun pepaya jepang. Tujuan dari penelitian ini adalah untuk menganalisis pengaruh konsentrasi dan lama waktu perendaman ekstrak daun pepaya sebagai antiparasit terhadap kadar glukosa darah dan tingkat konsumsi oksigen selama proses pengendalian Zeylanicobdella pada ikan kerapu cantang. Metode yang digunakan adalah metode eksperimental dengan rancangan acak lengkap faktorial 6 perlakuan dan 3 ulangan. Hasil dari penelitian ini yaitu diperoleh hasil rata-rata kadar glukosa yang diberi perlakuan ekstrak daun pepaya jenis berbeda selama dua jam menunjukkan bahwa pada faktor A dan B terdapat pengaruh dan interaksi yang sangat nyata (p<0,01) terhadap kadar glukosa darah, serta interaksi yang nyata (p<0,05) antara faktor A dan B terhadap tingkat konsumsi oksigen. Berdasarkan hasil penelitian dapat disimpulkan bahwa terdapat interaksi yang sangat nyata antara lama waktu perendaman dan konsentrasi ekstrak daun pepaya lokal dan pepaya jepang sebagai bahan antiparasit terhadap peningkatan kadar glukosa darah dan terdapat interaksi yang nyata terhadap tingkat konsumsi oksigen selama proses pengendalian Zeylanicobdella pada ikan kerapu cantang   Kata Kunci: Daun pepaya jepang, Daun pepaya lokal, Kerapu cantang,Zeylanicobdella     Abstract Efforts to increase grouper production are disease attacks. Alternative medicinal ingredients that can be used in disease control are using local papaya leaf and Japanese papaya leaf. The purpose of this study was to analyze the effect of concentration and duration of dipping in papaya leaf extract as an antiparasitic on blood glucose levels and oxygen consumption levels during the Zeylanicobdella control process in cantang grouper. The method used is an experimental method with a completely randomized factorial design with 6 treatments and 3 replications. The results of this study are the average results of glucose levels treated with different types of papaya leaf extract for two hours indicate that factors A and B have a very significant effect and interaction (p<0.01) on blood glucose levels, and significant interaction (p<0.05) between factors A and B on the level of oxygen consumption. Based on the results of the study, it can be concluded that there is a very significant interaction between the length of soaking time and the concentration of local papaya leaf extract and Japanese papaya as antiparasitic ingredients to increase blood glucose levels and there is a significant interaction with the level of oxygen consumption during the Zeylanicobdella control process in cantang grouper.   Keywords:Japanese papaya leaf, Local papaya leaf, Cantang grouper, Zeylanicobdella
Kadar Glukosa Darah dan Infestasi Ektoparasit Flagellata pada Ikan Mas Koki (Carrasius auratus) yang diberi Suspensi Whole Cell Lernaea dengan Dosis dan Waktu Pemeliharaan yang Berbeda Netty Sreani; Laksmi Sulmartiwi; Gunanti mahasri
Journal of Aquaculture Science Vol 8 No 1 (2023): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v8i1.241

Abstract

The high consumer interest in ornamental fish such as goldfish causes aquaculture to try to meet the highest production so that large scale aquaculture activities appear accompanied by problems due to imbalances between the host, the environment and diseases such as pathogens. The purpose of this study was to determine the effect of giving Whole Cell Lernaea as an immunostimulant development material as an effort to prevent pathogen attacks in aquaculture. This study used a completely randomized design (CRD) with a factorial pattern, namely factor A was different doses of Whole cell Lernaea and factor B was different length of maintenance. The results showed that the highest blood glucose levels of goldfish occurred in the 0 ppm treatment on day 0 with a range between 53.60-87.60 mg/dL and the lowest in the 5 ppm treatment on day 21 with a range between 44.20-86.60 mg/dL. Ichthyobodo spp. ectoparasite infestation. occurred on days 0 and 14 with a dose of 0 ppm which is about 2-3 ind/head different from giving a dose of 5 ppm during maintenance for 21 days there were no cases of goldfish that were attacked by ectoparasites. There was an interaction between dose and duration of maintenance on blood glucose levels of goldfish given Whole cell lernaea suspension, while there was no interaction with flagella ectoparasite infestation. The best treatment to reduce blood glucose levels to normal is the administration of Whole Cell Lernaea suspension at a dose of 5 ppm and a maintenance time of 21 days.
Cyprinus carpio hematological changes after dietary Coffea canephora leaf extract for Aeromonas hydrophila infection Shandy Sulistyoningrum; Gunanti Mahasri; Mohammad Faizal Ulkhaq; Hapsari Kenconojati
Depik Vol 12, No 2 (2023): AUGUST 2023 (IN PROGRESS)
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.28682

Abstract

Coffea canephora or robusta coffee was known as a medicinal plant that admitted various biological properties. However, its application as a medicinal plant in fish disease treatment has not been reported. This study aimed to investigate the effect of dietary robusta coffee leaf (RCL) extract on the survival rate and hematology of Cyprinus carpio infected by Aeromonas hydrophila. Two hundred fish were randomly divided into five treatment groups consisting of uninfected fish (UF), infected fish (IF), and a diet supplemented with different concentrations of RCL extract (30%, 40%, and 50%). The negative control group was injected by NaCl 0.9% and other treatment groups were injected by A. hydrophila. Feeding treatment was carried out for 7 days after clinical symptoms appeared. The results showed that RCL extract increases the survival rate and hematological parameters (number of erythrocytes, hemoglobin, and lymphocytes) significantly (P0.05). The highest survival rate (100%) was recorded in groups that accepted 50% of RCL extract. This current study indicates that RCL extract could be considered a potent medicinal plant for treating fish disease caused by A. hydrophila at the optimum dose of 50%.Keywords:AeromonasCoffea canephoraExtractHematologyCyprinus carpio
PENGARUH PUPUK FERMENTASI KARAGENAN TERHADAP DIAMETER SEL DAN KANDUNGAN LIPID Chlorella vulgaris SKALA SEMI MASSAL Alfin Tauhid; Luthfiana Aprilianita Sari; Gunanti Mahasri
Jurnal Teknologi Perikanan dan Kelautan Vol 14 No 2 (2023): NOVEMBER 2023
Publisher : Fakultas Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24319/jtpk.14.149-156

Abstract

Chlorella vulgaris merupakan salah satu mikroalga yang berpotensi digunakan sebagai bahan baku biodiesel. Selain itu juga digunakan sebagai food additive serta sumber pakan alami sehingga perlu dilakukan upaya kultur untuk memenuhi ketersediaan stok C. vulgaris. Kondisi lingkungan serta nutrien merupakan kondisi yang perlu diperhatikan. Salah satu sumber nutrien dapat diperoleh dari hasil fermentasi karagenan. Sedangkan kondisi lingkungan berkaitan dengan sistem kultur yang digunakan. Kultur skala semi massal digunakan untuk meningkatkan biomassa mikroalga. Penelitian tersebut menggunakan uji ANOVA dan uji jarak berganda Duncan dengan 4 perlakuan dan 5 ulangan yaitu P1 (Kontrol): menggunakan pupuk campuran Urea 70 ppm, ZA 40 ppm, TSP 40 ppm, EDTA 5 ppm, FeCl3 1 ppm, dan NPK 5 ppm dengan takaran pemberian disamakan dengan dosis pupuk Walne yakni 1 mL/L, P2: menggunakan pupuk karagenan yang difermentasi dengan dosis 20 ppm, P3: menggunakan pupuk karagenan yang difermentasi dengan dosis 30 ppm, dan P4: menggunakan pupuk karagenan yang difermentasi dengan dosis 40 ppm. Hasil penelitian diperoleh bahwa penambahan pupuk karagenan yang difermentasi diperoleh dosis terbaik 20 ppm dengan hasil diameter sel 2,79 µm dan kandungan lipid 45,02%.
Pengaruh Nanovaksin Berbasis Nanopartikel Hybrid Chitosan Terhadap Sel Darah Ikan Kerapu Cantang Yang Diinfeksi VNN Gian Suryanatha Hartawan; Uun Yanuhar; Muhammad Musa; Heru Suryanto; Gunanti Mahasri; Apri Supii; Nico Rahman Caesar
Jurnal Ilmu Pertanian Indonesia Vol. 28 No. 4 (2023): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.28.4.650

Abstract

Cantang grouper (Epinephelus sp.) is a fish with a high economic value and has been widely cultivated. VNN disase, caused by Betanodavirus infection, can lead to mass mortality of cantang grouper. Precautions considered effective in preventing Viral nervous necrosis (VNN) disease include vaccination. This study aimed to determine the effect of administering recombinant Chlorella vulgaris protein nanovaccine based on chitosan hybrid nanoparticles in increasing the immune response of cantang grouper infected with VNN. The study consisted of five treatments: K+ (cantang grouper infected with VNN), K- (healthy cantang grouper), T1 (healthy cantang grouper given 33 µl of nanovaccine and then challenged with VNN), T2 (healthy cantang grouper given 66 µl of nanovaccine and then tested with a VNN challenge), and T3 (healthy cantang grouper given 112 µl of nanovaccine and then tested with a VNN challenge). The parameters observed were haematology, nanovaccine Relative percent survival (RPS), and Survival rate (SR) levels. The study results showed that administering the nanovaccine increased the immune response of the fish, as indicated by an increase in haematology response, RPS, and SR in cantang grouper compared to the treatment without nanovaccine administration. The best dose of nanovaccine in this study is 33 µl. Keywords: fish disease, nanovaccines, virus, C. vulgaris, grouper
Effect of Whole Protein Spore Myxobolus koi by Oral Treatment on Non-Specific Immune Response of Punten Carp (Cyprinus carpio L.) Infested with Myxobolus koi Wiwin Sumiati; Gunanti Mahasri; Sri Subekti
Journal of Aquaculture and Fish Health Vol. 12 No. 3 (2023): JAFH Vol. 12 No 3 September 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i3.44446

Abstract

Increased intensive cultivation of Punten Carp (Cyprinus carpio L.) is quite profitable, but on the other hand, it can result in a decrease in water quality due to uncontrolled leftover feed. Poor water quality can cause fish to become stressed then susceptible to disease, one of which is Myxobolus koi parasite infestation. The purpose of this study was to determine the effect of immunostimulant treatment of whole protein Myxobolus koi spores on the blood profile of Myxobolus-infested punten carp. The method used was an experiment with two treatments, the treatment without Myxobolus koi spore whole protein (P0) and the treatment of commercial feed added with an immunostimulant from Myxobolus koi spore whole protein of 5 µg/kg feed (P1). The study was conducted for 28 days and observed on days 0, 7, 14, 21, and 28, including the calculation of the number of erythrocytes, leukocytes, leukocyte differential, and water quality as supporting data. The abundance of erythrocytes in fish treated with P1 (1.16x106 cells/mm3-2.44x106 cells/mm3) was higher than in fish treated with P0 (1.09x106 cells/mm3-1.55x106 cells/mm3). The abundance of leukocytes in fish treated with P1 (3.79x104 cells/mm3-11.31x104 cells/mm3) was higher than in fish treated with P0 (3.51x104 cells/mm3-6.58x104 cells/mm3). The results of differential observations of leukocytes in the P0 treatment found basophils (0.3-1.3%), neutrophils (12.3-21.5%), eosinophils (2.5-4.9%), lymphocytes (61.9-76.8%) and monocytes (3.8-6.3%). The results of differential observations of leukocytes in the P1 treatment found basophils (0.3-1.0%), neutrophils (10.7-19.9%), eosinophils (2.4-3.9%), lymphocytes (61.8-72.6%) and monocytes (3.9-5.4%).
Utilizing immunostimulants and probiotics for enhancing vannamei shrimp (Litopenaeus vannamei) aquaculture activities Syifania Hanifah Samara; Gunanti Mahasri; Rahayu Kusdarwati; Dheani Nur Ahadya Syifa Chaerunissa; Anggun Khoirun Nikmah; Elisabeth Benita Indarmastuti
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol 8, No 4 (2023): November 2023
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v8i4.11748

Abstract

Vannamei shrimp is one of the important fisheries commodities in Indonesia. This is because vannamei shrimp are expensive, popularly consumed by people around the world, and can be cultured. However, vannamei aquaculture activities are still hampered by disease, less production, and lack of technology implementation. Kedung Pandan Village (Jabon, Sidoarjo, East Java) is one of the potential areas for v vannamei shrimp aquaculture which is also constrained by this issues, so many farmers have gone out of business. This community service activity aims to increase the productivity of vannamei shrimp cultivation by administering immunostimulants and probiotics. In its implementation, this community service activity collaborates with "Putri Mandiri", a group of vaname shrimp farmers in Kedung Pandan Village. This activity is carried out in the stages of preparation, implementation of activities (socialization-demonstration), and evaluation.  Socialization and demonstration activities were carried out on August 9 2023 and were enthusiastically participated by residents. The results of this activity indicate that the knowledge of farmers in Kedung Pandan Village, Jabon, Sidoarjo has increased and can use immunostimulants and probiotics in vannamei shrimp farming activities.
Prevalensi dan Derajat Infestasi Ektoparasit pada Udang Vaname (Litopenaeus vannamei) di Tambak Intensif dan Tradisional di Kabupaten Gresik [ Prevalence and Degrees of Infestation Ectoparasite on White Shrimp (Litopenaeus vannamei) in Intensive and Extensive Cultivation System in Gresik] Gunanti Mahasri; Hari Suprapto; Abyan Farras
Jurnal Ilmiah Perikanan dan Kelautan Vol. 9 No. 2 (2017): Jurnal Ilmiah Perikanan dan Kelautan
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v9i2.7640

Abstract

                                                          AbstrakUdang putih (Litopenaeus vannamei) adalah komoditas unggulan budidaya yang memiliki harga yang signifikan pada pasar di seluruh dunia. Sepenuhnya permintaan udang, perlu perhatian serius dalam pembenihan vaname, dengan bertujuan untuk mendapatkan panen maksimal dan dapat memenuhi kebutuhan pasar. Tujuan penelitian ini untuk menentukan prevalensi dan derajat infestasi, serta mengetahui perbedaan prevalensi dan derajat infestasi ektoparasit pada udang vaname sistem intensif dan tradisional. Metode yang digunakan dalam penelitian ini menggunakan metode survei, yaitu metode pengamatan sistematis, faktual, dan akurat tentang fakta dan sifat populasi atau pada wilayah tertentu. Prevalensi ektoparasit yang menyerang udang putih di budidaya intensif 57,5% dan budidaya tradisional 56,6% dan dalam kategori sering (sering kali) tingkat infestasi rata - rata yang menginfeksi vaname putih dalam budidaya intensif adalah 76,56 (berat), sedangkan di budidaya tradisional adalah 43,78 (sedang). Hasil analisis data menunjukkan bahwa tidak ada perbedaan antara prevalensi budidaya intensif dan tradisional (p> 0,05), terdapat perbedaan tingkat infestasi ektoparasit antara prevalensi budidaya intensif dan tradisional                                                             Abstract White shrimp (Litopenaeus vannamei) is a species of cultivation and have important value in the worldwide market.Tofully a demand of shrimp, need for serious treatment in prawn hatchery vaname, with the aim that gets the maximum harvest and can fulfill a need market. The aims of this research to determine the prevalence and degree of infestation, as well as know the difference prevalence and degree of infestation ectoparasite on shrimps vaname which are in intensive and extensive cultivation. The method used in this study using survey methods, that is methods to make observation systematical, factual, and accurate about the fact and the nature of the population or to a definite region. The prevalence of ectoparasite that infests white shrimp in intensive cultivation is 57,5% and in traditional cultivation is 56,6% and in category frequently (often times). The average degree of infestation that infects white vaname in intensive cultivation is 76,56 (heavy), while in the traditional cultivation is 43,78 (medium). The results analysis of the data show that there is no difference between the prevalence of intensive and traditional cultivation (p > 0,05), there was difference infestation degree of ectoparasite between the prevalence of intensive and traditional cultivation.
Pengaruh Pemberian Ekstrak Sargassum sp. dengan Pelarut Metanol pada Pakan terhadap Jumlah Eritrosit dan Differensial Leukosit Ikan Lele Dumbo (Clarias gariepinus) [Effect Of Extract Sargassum sp. with Methanol Solvent in Feed on Erythrocythes and Differential Leucocythes of African Catfish (Clarias gariepinus) ] Firly Waliani Rahma; Gunanti Mahasri; Laksmi Surmartiwi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 2 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i2.11209

Abstract

Abstract The main obstacles in successful production is the attack of a disease. Preventive measures in reducing disease one of them is by means an increase in immunity the body of fish use natural compound (Parelberg et al., 2005). One of the natural compound that can be used as obtaining the immune system fish are the vitamin C, iodine, tannin (Bachtiar, dkk., 2012) and polysaccharides and derivatives as fucoidan. (Castro et. al., 2006) which is in Sargassum sp. With the provision of extract Sargassum sp. in feed is expected to optimize the number of erythrocytes and differensial leukocytes (number of neutrophils , monocytes and lymphocytes) at african catfish (Clarias gariepinus) so that it may optimize immunity the body of fish (Brown, 2000). The result of the observation of the blood of fish test is the use of extract Sargassum sp. wich get mixed in feed with doses 5, 10 and 15 g/kilogram feed during 21 days impact on the number of erythrocytes and prosentase lymphocytes blood catfishes. In doses C (15 g extract/ kilogram feed) put a value erythrocytes highest in day 21 namely 16.84 ± 1.56 x 105 cells/mm3, and give prosentase lymphocytes highest in day 21 than those wich others are 86.00 ± 3.81 %. But in a post test, B and C not seen the real difference between treatment. While for statistics the addition of extract not indicates the influence of real to the percentage neutrophils and monocytes of the blood fish. So that it can be said that addition extract Sargassum sp. with doses 15 g extract/kilogram feed give the effectiveness of the optimal response immune system than second doses other.
Gambaran Darah dan Histopatologi Insang, Usus Dan Otak Ikan Koi (Cyprinus carpio Koi) yang Diinfeksi Spora Myxobolus koi secara Oral [Haematology and Histopatology of Gills, Intestine And Brain Koi Fish (Cyprinus carpio Koi) Myxobolus koi Orally Infected] Gunanti Mahasri; Kusnoto Kusnoto; Era Insivitawati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 2 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i2.11210

Abstract

Abstract Koi fish (Cyprinus carpio koi) is one of the ornamental fish are preferred. One of the obstalces in the koi fish is a disease caused by infection myxobollus. This research to determine the effect of the infection myxobollus by oral in blood picture and histopathology. Haematology and histopathology is an indicator of the health of the fish to diagnose disease and to determine changes in the fish tissue. The research was conducted in Politeknik Kelautan dan Perikanan, dan Balai Karantina Ikan Kelas I, Juanda Surabaya from October to December 2014. This study uses the 5 treatments and 5 replications with different doses of infection (0,60,80,100 and 120 spora/fish). Data were analyzed with Annova haematology, followed by Duncan 5%. While histopathology were analyzed by semiquantitative scoring mothod used to determine the degree of histology Kluschal Wallis. The result showed that the haematology has significant result, white histopathology signify the presence of significant damage.
Co-Authors A. Shofy Mubarak Abdul Manan Abdul Manan Abyan Farras Adamu Ayubu Mwendolwa Adamu Ayubu Mwendolwa Adiacahya, Eren Aditya Gita Rohmatullah Adriana Monica Sahidu Agung Pamuji Rahayu Agus Nazarudin Yahya Ahmad Shofy Mubarak Akhmad Taufiq Mukti Alanosi Noor Muhammad Alfan Prianggara Alfin Tauhid Alim Isnansetyo Almira Fardani Lahay Alvira Febrianti Pratiwi Anam, M Khairul Anggun Khoirun Nikmah Anggun Nurani Citrowati Anisa, Hosia Anord Charles Nkuba Apri Supii Ardilas Heryamin Arika Juniarsih Arya Witantama Bahtiar, Sadida Anindya Berliana A Bernathdo Mahendra Robbi Putra Bidayatul Afifah Boedi S. Rahardja Boedi Setya Rahardja Browijoyo Santanamurti Cintia Larasati DARMAWAN SETIA BUDI Desak Ketut Sekar Cempaka Putri Dewi, Nina Nurmalia Dheani Nur Ahadya Syifa Chaerunissa Dieswinta Hardika Aris dika dika Dita Wisudyawati Eka Saputra Elangga Sony Widiharsono Elisabeth Benita Indarmastuti Endah Sih Prihatini Endang Dewi Masithah Era Insivitawati Ewang Mahendra Putra Fahdi Putra Utama Faisol Mas&#039;ud Faisol Mas’ud Farid Nur Salim Farizka Vinka Trinendyah Ferry Dwi Firmansyah Liananda Firly Waliani Rahma Fuad Fuquh Rahmat Shaleh Gian Suryanatha Hartawan Hari Suprapto Heru Suryanto Ika Purnamasari Ika Putri, Lia Oktavia Ikmalia A Ikmalia Amali Indah Hidayati Imani Iqbal Ghazali Irvansyah Irvansyah Isroni, Wahyu Kadek Racmawati Karunia, Fitria Kenconojati, Hapsari Kismiyati , Koesnoto Koesnoto Kusnoto Kusnoto Lailatul Lutfiyah, Lailatul Laksmi Sulmartiwi Laksmi Surmartiwi Larasati, Anastasya Dewi Lestari Wilujeng Lia Oktavia Ika Putri Lia Oktavia Ika Putri Lilis Cahaya Septiana Linnya Prima Agustin Lucia Tri Suwanti, Lucia Tri Luthfiana Aprilianita Sari Lyla Wulandari M Ervany Eshmat M Iqbal Satria Mandele, M. Muhtar Mayangsari, Cholivia Melinda Kusuma Ningrum Miftakhul Munir Moch Saad Mochammad Dwi Hardhianto Mohamad Yusuf Mohammad Faizal Ulkhaq Mufasirin Muhamad Amin Muhamad Amin Muhammad Amin Muhammad Arief Muhammad Herman Muhammad Hilmy Maulana Muhammad Musa N. Juni Triastuti Nafis Putra Laksana Cholil Nanuk Qomariyah Nedi Nedi Netty Sreani Nico Rahman Caesar Niluh Suwasanti Norma Isnawati Nugroho, Sefilia Adhi Nunuk Dyah Retno Lastuti Nur Fais Nurlita Abdulgani Nurul Kumalasari Ochtavia, Sherly Permata Jelita, Chelsea Prayogo Prayogo Pristita Widyastuti Puguh Yugo Wijanarko Putra, Bernathdo Mahendra Robbi Putri, Desak Ketut Sekar Cempaka Rachma Woro Anggarani Racmawati, Kadek Rahayu Kusdarwati Rahayu Kusdarwati Ridwansyah Ririn Agustiya Riris Ulfiana Riza Aryani Romziah Sidik Rozi Rozi Rr. Juni Triastuti Salman Aldo Alfaresi Samara, Syifania Hanifah Santanumurti, Muhammad Browijoyo Sapto Andriyono Saputra, Alfian Rahmadhani Satria Hani Sari, Putri Desi Wulan Saroso, Heri Setiawan Koesdarto Shandy Sulistyoningrum Sherly Ochtavia Shohifah, Isnatul Umu Siti Hamidah Sri Mulyati Sri Mulyati Sri Subekti Sri Subekti Sri Subekti Sudarno Sudarno Sudarno, Sudarno Sulistyoningrum, Shandy Suwasanti, Niluh Suzanita Utama Titom Gusmana Putra Perdana Ulia Fajriah Veryl Hasan W. Angan Indrawan Widodo, Langgeng Wijanarko, Puguh Yugo Wiwin Sumiati Wiwin Sumiati Woro Hastuti Satyantini Woro Hastuti Setyantini Wurlina, W Yanuhar, Uun Yudi Cahyoko Yunifar Amad