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Phytochemical Screening Analysis and Determination of Total Flavonoids and Total Phenolics Content of Ethanol Extract of Sungkai Leaf (Penorema canescens Jack) from Samarinda City Muhammad Ma'ruf; Moch. Saiful Bachri; Laela Hayu Nurani
Jurnal Mandala Pharmacon Indonesia Vol. 9 No. 2 (2023): Jurnal Mandala Pharmacon Indonesia
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v9i2.360

Abstract

Sungkai is a plant that contains compounds such as flavonoids, tannins, phenolics, saponins, steroids, and terpenoids, which have the potential to develop native Indonesian herbal medicines to treat the prevention and treatment of various diseases. This research aimed to analyze phytochemical screening and the total flavonoid and phenolic content in sungkai leaves originating from Samarinda City. This research method was carried out by extracting sungkai leaves with 96% ethanol and evaporating them with a vacuum rotary evaporator. Sungkai leaf extract was analyzed for phytochemical screening using the tube method with color testing from various reagents and total phenolic and flavonoid content testing using the colorimetric method using a UV-Vis spectrophotometer. The results of the phytochemical screening showed that the ethanol extract of sungkai leaves from Samarinda City was positive for containing flavonoids, saponins, alkaloids, phenolics, and steroids. The results of determining the flavonoid and phenolic content of sungkai leaf ethanol extract were 18,210 ± 0.271 mg QE/g sample and 33,666 ± 1.052 mg GAE/g sample.
SKRINING KESEHATAN JIWA, RELIGIUSITAS, DAN KUALITAS HIDUP MAHASISWA FARMASI DAN KEDOKTERAN PADA PANDEMI COVID-19: SCREENING OF MENTAL HEALTH, RELIGIUSITY, AND QUALITY OF LIFE OF PHARMACEUTICAL AND MEDICAL STUDENTS IN THE COVID-19 PANDEMIC Widea Rossi Desvita; Wiwara Awisarita; Muhammad Fikri; Akrom; Laela Hayu Nurani; Isabella Meliawati Sikumbang
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 7 No 2 (2022)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v7i2.342

Abstract

Kesehatan mental mahasiswa Farmasi dan Kedokteran perlu diskrining terhadap keberadaan COVID-19. Faktor internal yang perlu diperhatikan adalah aspek religiusitas dan kualitas hidup. Instrumen yang dikembangkan untuk pemeriksaan kesehatan mental adalah SRQ29 serta religiusitas dan kualitas hidup dengan EQ5D. Tujuan dari penelitian ini adalah untuk mengetahui kesehatan mental dan kualitas hidup mahasiswa Farmasi dan Kedokteran pada masa pandemi COVID-19. Penelitian ini merupakan penelitian cross sectional diskriptif dengan metode survey terhadap 44 responden yang meliputi 36 mahasiswa Fakultas Farmasi dan 8 mahasiswa Fakultas Kedokteran. Metode yang digunakan untuk skrining kesehatan jiwa adalah instrumen kuesioner yaitu SRQ-29 untuk skrining kesehatan jiwa dan religiusitas yang berisi 29 pertanyaan terkait kesehatan jiwa responden, dan skrining kualitas hidup dengan instrumen EQ5D. Hasil penelitian menunjukkan bahwa dari skrining SRQ29 mahasiswa farmasi dan FK  perguruan tinggi di Yogyakarta mengalami gejala PTSD (75%), cemas dan depresi (61,36%), gangguan psikotik (50%), penggunaan zat psikoaktif/ narkoba (2,27%). Berdasarkan hasil skrining religiusitas menunjukkan nilai yang tinggi terhadap keyakinan dan iman kepada Allah SWT (96,8%). Hasil EQ5D menunjukkan bahwa pandemi tidak berdampak besar pada aspek fisik, namun berdampak pada mental/ psikologis. Kesimpulan penelitian terkait kesehatan jiwa pada hasil skrining dengan instrumen SRQ29 menunjukkan bahwa mahasiswa farmasi dan FK  perguruan tinggi di Yogyakarta mengalami masalah psikososial.  Hal ini saat dikaitkan dengan religiusitas dan kualitas hidup menunjukkan adanya pengaruh faktor internal tersebut pada kesehatan jiwa.
ANALISIS PROFIL MINYAK DAUN CENGKEH (Syzygium aromaticum (L.) Merr. & L. M. Perry) DAN PRODUK DI PASARAN : PROFILE ANALYSIS OF CLOVE LEAF OIL (Syzygium aromaticum (L.) Merr. & L. M. Perry) AND PRODUCTS IN THE MARKET Any Guntarti; Putri Lestari; Laela Hayu Nurani
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 8 No 1 (2023)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v8i1.534

Abstract

Minyak cengkeh memiliki nilai komersial dan manfaat yang besar serta permintaan ketersediaan minyak cengkeh di pasaran sangat tinggi, namun hasil ekstraksi minyak cengkeh cukup rendah sekitar 2-3% sehingga dapat menimbulkan motif untuk mengeksplorasi pasokan minyak daun cengkeh dengan tindakan pemalsuan. Tujuan dari penelitian ini untuk menganalisis secara kualitatif profil dan kemurnian minyak daun cengkeh dan produk minyak daun cengkeh di pasaran berdasarkan persyaratan mutu dari Standar Nasional Indonesia (SNI). Jenis penelitian ini berupa penelitian non eksperimental. Minyak daun cengkeh dan minyak produk A, B dan C dilakukan uji kualitas minyak berdasarkan syarat uji SNI 06-2387-2006 yang meliputi organoleptis (warna dan bau), bobot jenis, indeks bias dan kelarutan dalam etanol 70%. Selanjutnya dilakukan analisis KLT dengan menggunakan fase diam silika gel GF254 dan fase gerak Toluen : Etil Asetat (93:7). Hasil destilasi minyak daun cengkeh sebanyak 3 Kg dengan destilasi uap air  didapatkan rendemen sebesar 0,83%. Hasil analisis profil minyak menunjukkan bahwa minyak daun cengkeh dan minyak produk A memenuhi syarat uji SNI 06-2387-2006, sedangkan pada minyak produk B dan C terdapat beberapa parameter pengujian yang tidak memenuhi syarat uji di antaranya bobot jenis, indeks bias dan kelarutan dalam etanol 70%, sedangkan pada hasil uji dengan KLT menunjukkan pola noda yang sama dengan nilai Rf sebesar 0,63.
QUALITY ANALYSIS AND IDENTIFICATION OF FUNCTIONAL GROUPS OF SWEET ORANGE (Citrus sinensis) PEEL ESSENTIAL OIL USING FTIR Citra Dhea Cantika; Laela Hayu Nurani; Nina Salamah
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 8 No 4 (2023)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v8i4.898

Abstract

Organic waste in the form of citrus fruit peels has not been handled seriously, causing environmental pollution, such as the emergence of unpleasant odors.  Therefore, waste management becomes a more valuable product or item.  Citrus peel oil counterfeiting often occurs because there is no Indonesian National Standard (SNI) for citrus peel oil as a quality standard, making it difficult to identify the falsity of finished citrus peel oil products in the market. Research was conducted to test the quality of sweet orange peel oil so that it could be used as a standard to identify the content of sweet orange peel oil using the FTIR method. Oil quality testing included an organoleptic test, specific gravity, refractive index, and acid number. The oil quality test results showed clear yellow oil with a distinctive sweet orange peel aroma, specific gravity of 0.845, refractive index of 9.263, and acid number of 1.1. FTIR analysis revealed spectra that appeared in the region of 1490 with strong intensity (C-H aromatic), 1645 with medium intensity (C=C), and 2860 with medium intensity (C-H aliphatic). FTIR spectra of sweet orange peel oil showed similarity with limonene with a hit quality value of 958, the content of sweet orange peel oil is dominated by limonene compounds. Keywords: sweet orange peel, Essential oil, FTIR, Limonene, Oil quality
THE APOPTOSIS INDUCTION OF Zingiber oficinale ETHANOLIC EXTRACT-Treated HeLa (HUMAN CERVICAL CANCER) CELLS AND ACTIVE COMPOUND PROFILING USING GAS CHROMATOGRAPHY/MASS SPECTROMETRY Laela Hayu Nurani; Siti Rofida; Dwi Utami; Citra Ariani Edityaningrum; Any Guntarti
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 9 No 1 (2024)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v9i1.1059

Abstract

Cervical cancer accounts for the highest percentage of cancer-related deaths in Indonesia, accounting for nearly 60% of all cancer cases.  Therefore, research into the anticancer mechanisms needs to be conducted.  The ethanolic extract of Zingiber officinale (EEZO) contains zingiberene, a chemical known for its anticancer activity. Understanding the mechanism underlying the apoptosis-inducing effects is crucial. This study aimed to elucidate the apoptotic pathway and analyze the gas chromatography/mass spectrometry (GC/MS) profile of EEZO cells. The research commenced with the maceration of Zingiber officinale rhizomes using 75% ethanol to obtain EEZO. Apoptosis assays were conducted on both a negative control group and an EEZO-treated group of HeLa cells (cervical cancer cells). The apoptotic mechanism was evaluated using forward scattered light-side scattered light (FSC-SSC), fluorescein isothiocyanate (FTIC), and phycoerythrin (PE) flow cytometry. Apoptotic results were analyzed by comparing the control and EEZO samples, which revealed the number of viable cells, apoptotic cells, and cells in the sub-G1 phase. The major constituent of EEZO, which is expected to be a potent apoptosis inducer, was detected using GC/MS. The FSC-SSC results indicated a lower number of viable cells in the EEZO-exposed group than in the control group. FTIC results demonstrated that EEZO significantly increased apoptotic cell death, increasing from 68 to 1537 cells. PE flow cytometry revealed an elevated sub-G1 cell population, indicating the induction of apoptosis by EEZO. GC/MS analysis revealed five dominant components in EEZO, which had the potential to induce apoptosis: L-borneol, zingiberene, farnesol, beta-sesquiphellandrene, and alpha-curcumene ...  
Analisis Profil Minyak Atsiri Daun Kayu Putih (Melaleuca leucadendra L.) dan Produk di Pasaran Irfan, Nawwar; Nurani, Laela Hayu; Guntarti, Any; Salamah, Nina; Edityaningrum, Citra Ariani
Journal of Food and Pharmaceutical Sciences Vol 10, No 3 (2022): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.5785

Abstract

Minyak kayu putih merupakan salah satu minyak atsiri yang biasa digunakan dalam sehari-hari. Produksi dalam negeri hanya dapat memproduksi minyak kayu putih 650 ton/tahun dari permintaan kebutuhan dalam negeri dalam setahun sebesar 3.500 ton sehingga memacu pemalsuan. Penelitian ini bertujuan untuk membandingkan profil minyak kayu putih hasil destilasi dengan minyak kayu putih yang ada di pasaran berdasarkan mutu Standar Nasional Indonesia (SNI). Destilasi minyak atsiri dilakukan dengan 5 kg daun kayu putih menggunakan metode destilasi uap air selama 3 hari didapatkan rendemen sebesar 2,32%. Minyak kayu putih hasil destilasi dan minyak produk A, B dan C dilakukan pengujian profil minyak berdasarkan SNI 06-3954-2006 meliputi warna, bau, bobot jenis, indeks bias, kelarutan dalam etanol 70%, dan profil KLT. Hasil pengamatan organoleptis minyak hasil destilasi, produk A, B, dan C memiliki perbedaan warna, bobot jenis, dan kelarutan dalam etanol 70% serta tidak ada perbedaan bau dan indeks bias. Hasil penelitian menunjukkan adanya perbedaan profil minyak produk A, B, dan C yang tidak memenuhi SNI pada pengujian bobot jenis pada produk C. KLT dengan menggunakan fase diam silika gel GF254 dan fase gerak Toluen (100%). Profil KLT pada produk C tidak terlihat noda yang sama dengan standar eucalyptol yang diperlihatkan oleh minyak lainnya. Keywords: Analisis profil, Minyak kayu putih, SNI 06-3954-2006
Enhancing Anticancer Potential: Investigating the Synergistic Impact of Doxorubicin and Curcumin on HeLa and Vero Cells in Vitro Aristiani, Windy; Desvita, Widea Rossi; Khayatulisma, Dika; Edityaningrum, Citra Ariani; Guntarti, Any; Nurani, Laela Hayu
Journal of Food and Pharmaceutical Sciences Vol 12, No 1 (2024): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.10887

Abstract

(1) Background: Cervical cancer ranks as the fourth most prevalent cancer globally and in Asia, standing as the second most common in Indonesia. Despite its efficacy, doxorubicin chemotherapy is associated with significant side effects. To mitigate these adverse effects, a promising approach involves combining conventional drugs with curcumin. Both curcumin and doxorubicin have demonstrated cytotoxic effects against cervical cancer (HeLa). This research aims to determine the synergistic effect of the combination of doxorubicin and curcumin on HeLa and safety in normal cells (Vero cells). (2) Methods: This study adopted an experimental design utilizing doxorubicin and curcumin samples with HeLa and Vero cells. The investigation was initiated with cytotoxic and combination tests using the MTT method. The obtained results included IC50 values and combination indices, and the analysis involved a comparative examination of outcomes between HeLa and Vero cells. (3) Results: Cytotoxic tests revealed IC50 values for doxorubicin and curcumin on HeLa cells, measured in 2,17 ± 0,06 and 26,37 ± 2,00 µg/mL, and 17,32 ± 1,99 and 181,88 ± 30,48 µg/mL on Vero cells. Combination test results were represented by combination index .(4) Conclusions: The synergistic effect is observed at the combination of curcumin at a concentration of 9 μg/mL and doxorubicin at a concentration of 0,125 μg/mL, resulting in a combination index of 0,50. These findings suggest a promising avenue for enhancing the therapeutic potential of doxorubicin in cervical cancer treatment while minimizing adverse effects.
The Hepatoprotective Effect of Moringa oleifera Leaves on Male Wistar Rat Induced Streptozotocin-Nicotinamide Bachri, Moch Saiful; Nurani, Laela Hayu; Edityaningrum, Citra Ariani; Ma'ruf, Muhammad; Sulistyorini, Dwi Agustin; Amelia, Rizki; Hidayati, Sholihatil; Sulistyawati, Rini; Guntarti, Any
Majalah Obat Tradisional Vol 29, No 2 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.90806

Abstract

Diabetes mellitus is a disease characterized by uncontrolled increases in blood sugar levels, which can cause complications in the liver. Disorder liver disease can be seen from the increasing SGOT, SGPT activity, and the number of necrosis cells in the liver. Moringa leaves contain the flavonoid quercetin with antidiabetic and antioxidant activity. This research aimed to determine the effect of administering the ethyl acetate fraction of Moringa leaves on SGOT activity, SGPT, and liver histopathology in rat induced by streptozotocin and nicotinamide. Moringa leaves macerated with 80% ethanol and fractionated with ethyl acetate were used. The research subjects were 30 rats divided into six groups. The rats were induced with streptozotocin at a dose of 65 mg/kgBW and nicotinamide 100 mg/kgBW for five days, then treated for ten days. Serum SGOT and SGPT activity were measured using a Microlab 300 Semi-Automated at a wavelength of 340 nm. Histopathological observations were carried out when the rats were dislocated, their livers were taken, and then preparations were made using Hematoxylin Eosin (HE) staining. Data were analyzed using the SPSS One Way ANOVA method, followed by the Tukey test with a confidence level of 95%. The results of the study showed that administration of Ethyl Acetate Fraction of Moringa oleifera (EA-MO) could reduce the levels of SGOT, SGPT, and the number of liver necrosis cells in rats induced by streptozotocin and nicotinamide.
AUTHENTICATION OF PATCHOULI OIL FROM VARIOUS GROWING REGIONS USING GC-MS METHOD WITH CHEMOMETRIC COMBINATION ON THE PRODUCTS IN THE MARKET Nurani, Laela Hayu; Guntarti, Any; Milanie, Rida Dwi; Gandjar, Ibnu Gholib; Rais, Ichwan Ridwan; Prasasti, Dian; Edityaningrum, Citra Ariani
Jurnal Farmasi Sains dan Praktis Vol 10 No 1 (January-April 2024)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v10i1.10523

Abstract

Adulteration in patchouli oil trade is an ancient practice, where additional substances like turpentine oil are added to increase volume and odor. Therefore, this research aimed to determine the composition of patchouli oil, considering variations in growing regions and potential adulteration in the products available in the market. The analysis was carried out using the Gas Chromatography-Mass Spectrormetry (GC-MS) method with a Principal Component Analysis (PCA) chemometric combination for authentication. The sample obtained from Bantul, Kulon Progo, and Purworejo was isolated through steam and water distillation. Subsequently, it was tested in accordance with SNI standard 06-2385-2006 and analyzed using GC-MS. The results were compared to the products on the market and processed using multivariate chemometric, namely PCA with Minitab 19 software. GC-MS analysis showed the presence of various compounds contained in the oil, including alcohol, alpha-guaiene, beta-humulene, seychellene, trans-caryophyllene, neoalloocimene, and beta-patchoulene. The combination of GC-MS and PCA chemometrics was able to distinguish patchouli oil from others and the products available on the market. PCA chemometric analysis showed that the patchouli oil from various growing regions had the same chemical components as essential oil. Furthermore, PCA chemometric analysis of market products also showed similar results but varied significantly from turpentine oil. This showed that the patchouli oil product available on the market did not contain turpentine oil.
OPTIMIZATION OF JAMBLANG (Syzygium Cumini (L.) Skeels) FRUIT EXTRACT LEVELS IN YOGHURT FORMULATION AS ANTIOXIDANT Yanuarto, Tri; Nurkhasanah; Hayu Nurani, Laela
PHARMACOLOGY, MEDICAL REPORTS, ORTHOPEDIC, AND ILLNESS DETAILS Vol. 1 No. 1 (2022): JANUARY
Publisher : Transpublika Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (519.752 KB) | DOI: 10.55047/comorbid.v1i1.55

Abstract

The reactivity of oxidant compounds that exceed the limits of antioxidant protection ability can cause chain reactions which cause interference with the system. Prevention is needed to neutralize the oxidants, so that exogenous antioxidants are needed. One example is the jamblang fruit (Syzygium cumini (L)) which has high antioxidant activity due to its natural anthocyanin. The purpose of this study was to determine the optimal form of yogurt with the addition of jamblang fruit extract with a concentration of 0%, 5%, 7.5% and 10% and the quality of SNI. Tests carried out include physical, chemical and microbiological tests, namely sensory, acid content, total protein, fat content, and a number of bacterial starters, as well as safety tests with storage at 4° C then 0, to 7, 14th, 21st and 28th day. Data analysis using MANOVA. The results of the effect of the product showed that the fruit of yogurt with 10% extract level provided good stability during storage. Decreased antioxidant levels on day 7 to day 28. Antioxidant activity is more stable than antioxidant jamblang fruit extract. The antioxidant activity of yogurt with 5% jamblang fruit extract; 7.5% and 10% on day 0% % inhibition value of 57.48%; 62.06%; and 73.01%.
Co-Authors Abdul Rohman Abdul Rohman Abdul Rohman Abdul Rohman Achmad Mursyidi Achmad Mursyidi Afiana Rohmani Agitohutomo, Muhammad Ainiyah, Nadia Putri Aisyah, Vani Aisyah, Vani Akrom Akrom, Akrom Aldri Frinaldi ANDI WIJAYA Anggita Rosiana Putri Anjar Windarsih Annisa Fatmawati Any Guntarti Any Guntarti Any Guntarti Any Guntarti Any Guntarti Arif Santoso Aris Asahi Aristiani, Windi Aristiani, Windy Astuti, Ratih Arum Azzahra, Fara Bachri, Mochammad Saiful Citra Ariani Edityaningrum, Citra Citra Dhea Cantika Danang Prasetyaning Amukti Daru Estiningsih Daud, Intan Devi Kumala Dewi Dewi Andini Kunti Mulangsri, Dewi Andini Kunti Dewi, Devi Kemala Dian Prasasti Djannah, Sitti Nur Djannah, Sitti Nur Dwi Utami Edityaningrum, Citra Ariyani Efiana, Nuri Ari Eka Kumalasari Eka Kumalasari Eka Kumalasari Eka Sakti Wahyuningtyas Eka Wahyuning Tyas Elya Zulfa, Elya Endang Darmawan Eti Nurwening Sholikhah Eti Nurwening Sholikhah Fara Azzahra Fara Azzahra Fauziyya, Riri Feri Anggita Hastanto Feri Anggita Hastanto Fikri Achmad Arif Fitriyati, Laeli Galih Dwi Mulyati Gela Setya Ayu Putri Hari Susanti Heni Lutfiyati, Heni Heni Setyowati Esti Rahayu Hidayati, Sholihatil Husnun Khairunnisa Pratiwi Ibnu Gholib Gandjar Ibnu Gholib Gandjar Ibnu Gholib Gandjar Ichsan Luqmana Indra Putra, Ichsan Luqmana Indra Ichwan Ridwan Rais Iin Narwanti Iin Narwanti Iis Wahyuningsih Intan Rahayu Irfan, Nawwar Irnawati Irnawati Isabella Meliawati Sikumbang Isabella Meliawati Sikumbang Jumina Jumina Jumina Jumina Kartikadewi, Arum Khairurrizki, Amanda Khayatulisma, Dika Kurnianto, Erwan Lalu Muhammad Irham Mahardika Agus Wijayanti Ma’ruf, Muhammad Meta Safitri Milanie, Rida Dwi Miratun Syarifah Moch Saiful Bachri Moch. Saiful Bachri Moch. Saiful Bachri Moch. Saiful Bachri Muhammad Fikri Muhammad Ma'ruf Muhammad Yusron Maulana El-Yunusi Mustofa Mustofa Nanik Sulistyani Nasruddin Nasruddin Nasruddin Nasruddin Nasruddin, N Nina Salamah Nining Sugihartini Nining Sugihartini Nugrahini, Febrina Nur Azizah Nur Ismiyati Nurkhasa Nurkhasa, Nurkhasa Nurkhasanah Nurkhasanah Mahfudh Nurkhasanah Nurkhasanah Putri Kurniasiwi Putri Lestari Rahma Dona Ramadhan, Muhammad Marwan Rini Sulistyawati Rizki Amelia Rohman, Abdul Salsabila, Nia Sapto Yuliani SATRIYAS ILYAS Sikumbang, Isabella Meliawati Sitarina Widyarini Sitarina Widyarini Siti Fatmawati Fatimah, Siti Fatmawati Siti Rofida Siti Salma Yusuf Siti Setianingsih Sitti Nur Djannah Sudjadi . Sugiyanto - Sulistyorini, Dwi Agustin Suwidjiyo Pramono Syarif, Yaumi Musfirah Titiek Suhardi Haripurnomo Kushadiwijaya Hidayati Tri Ani Marwati Tri Yanuarto Tria Zakinah Utari, Dina Vani Aisyah Vivi Sofia Wahyu Widyaningsih Wahyuningtyas, Nurma Widea Rossi Desvita Widyarini, Sitarina Wihasty Nur Istyqomah Wiwara Awisarita Yaumi Musfirah Syarif Yeni Dianita Yeni Yeni, Yeni Dianita Yeni, Yeni Dianita Yoga Yuniadi Yusuf, Siti Salma Zainab Zainab Zainab Zainab