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Efek flavonoid daun srikaya (Annona squamosa (L)) terhadap pemacuan ekspresi P53 sel HeLa Laela Hayu Nurani; Suwidjiyo Pramono; Sudjadi .
Indonesian Journal of Pharmacy Vol 22 No 4, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm0iss0pp273-278

Abstract

Flavonoids  are  secondary  metabolites  with  many  activities  such  as  an anticancer  and  an  antioxidant. Annona  squamosa L.  leave  are  reported  contain flavonoids. Annona squamosa flavonoids were isolated from ether fraction and separated by paper chromatography with acetic acid 15% as mobile phase. Four flavonoids   with  Rf  value  of  0.11,  0.23,  0.46  and  0.78  were  detected  as  5-hydroxy-7-R-flavonol  (1),  5,3’,4’-trihydroxy-7-R-flavonol  (2),  5-hydroxy-7-Rflavanone  (3)  and  7-R-flavanone  (4),  respectively.  The  cytotoxicity  of  each flavonoid  on  Hella  cell  line  was  carried   out   using  direct  counting  method  and the p53 expression was done with immunocytochemistry. The result showed that LC50  of  flavonoid 1, 2, 3 and  4 was  12.63±3.4,  18.47±3.4,  75.74±6.2  and 204.4±14.0  µg/mL.   Twelve   µg/mL  of  5-hydroxy-7-R-flavonol  (1)   increased p53 expression of eleven fold than control.Keywords: Annona squamosa, HeLa, flavonoid, p53 
Cytotoxic Potential of Arthrospira platensis Extract on Cervical Cancer Cells Line Hela: Study on Antiproliferative, Cell Cycle, Apoptosis Induction and Anti Metastasis Laela Hayu Nurani; Nurkhasanah Mahfudh; Ibnu Gholib Gandjar; Intan Rahayu
Indonesian Journal of Pharmacy Vol 31 No 1, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss1pp19

Abstract

Cervical cancer can be treated conventionally with chemotherapy agents, but its use has side effects and complications in the form of damage to normal cells. This study aims to determine the potential of A. platensis as an alternative anticancer agent that is selective towards normal cells. Based on TLC analysis, A. platensis contains antioxidant compounds such as β-carotene, flavonoids, and terpenoids which are able to inhibit proliferation and trigger apoptosis of cancer cells. The study was conducted using cervical cancer cells HeLa and normal cells HDFa. A. platensis macerated with 96% ethanol at a ratio of 1:4. Based on probit analysis, it is known that ethanol extract of A. platensis has a cytotoxic effect on HeLa cells with IC50 values of 260.444μg/mL and index selectivity towards HDFa cells of 7.931. The mechanism of cytotoxic activity of ethanol extract of A. platensis is related to its ability to extend the doubling time, increase the induction of apoptosis, and reduce the rate of cells migration. Ethanol extract of A. platensis can also increase cells accumulation in the S phase to prevent cells from entering the G2/M phase.
Efek Kapsul Ekstrak Etanol Kelopak Bunga Rosella (Hibiscus sabdariffa L.) terhadap Kadar Bilirubin Sukarelawan Sehat Nur Azizah; Endang Darmawan; Laela Hayu Nurani
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 4 No. 1 (2017): Jurnal Farmasi dan Ilmu Kefarmasian Indonesia
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.832 KB) | DOI: 10.20473/jfiki.v4i12017.13-18

Abstract

Pendahuluan: Hibiscus sabdariffa adalah salah satu ramuan tradisional yang memiliki banyak manfaat termasuk efek imunostimulan, karena banyak mengandung antioksidan di dalamnya termasuk anthocyanin dan flavonoid. Berdasarkan aktivitasnya pada tes praklinis, H.sabdariffa telah memenuhi syarat untuk melanjutkan ke fase uji klinis. Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh kapsul ekstrak etanol kelopak bunga rosella (Hibiscus sabdariffa L.) pada kadar bilirubin  11 sukarelawan sehat  pria dan 10 sukarelawan sehat wanita. Metode: Penelitian ini menggunakan rancangan pre-post treatment. Sukarelawan sehat mengkonsumsi kapsul ekstrak etanol kelopak bunga rosella selama 30 hari dengan dosis 500 mg sekali sehari pada malam hari. Tes Bilirubin dilakukan pada hari ke-0; 31 dan 45, menggunakan metode Jendrassik dan Grof. Hasil dianalisis menggunakan SPSS dengan paired t-test dan uji Wilcoxon. Hasil: Perbandingan nilai rata-rata pada hari ke-0 dan 31, dengan hari ke-31 dan 45, dan perbandingan nilai rata-rata antara hari ke-0 dan ke-45 tidak menunjukkan perbedaan yang signifikan pada kadar bilirubin total dan direct (p > 0,05), sedangkan perbandingan nilai rata-rata untuk kadar bilirubin indirect hari ke-0 dan ke-45 menunjukkan adanya perbedaan yang signifikan (p = 0,05) akan tetapi kadar bilirubin indirect tersebut masih dalam rentang nilai normal. Kesimpulan: Kapsul ekstrak etanol kelopak bunga rosella tidak mempengaruhi kadar bilirubin total, direct dan indirect pada sukarelawan sehat.
EFEK KO-KEMOTERAPI FRAKSI ETIL ASETAT AKAR PASAK BUMI DAN DOXORUBICIN TERHADAP PROLIFERASI DAN EKPRESI BAX JARINGAN PAYUDARA TIKUS SD Tria Zakinah; Laela Hayu Nurani; Sitarina Widyarini
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 14, No 1 (2017)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (741.051 KB) | DOI: 10.24071/jpsc.00559

Abstract

The use of co-chemotherapy agent was needed since there were some toxicities on the normal tissues caused by the use of doxorubicin. The root of Eurycoma longifolia Jack, a part of plant, has a potential activity as co-chemotherapy. This study aimed to determine the effect of co-chemotherapy of ethyl acetate fraction of E. longifolia Jack roots and doxorubicin against cell proliferation activity by AgNOR method and determine expression of Bax protein of breast tissue in rats induced by DMBA. The rats were divided into five groups: I (baseline), II (DMBA 20 mg/kgBW), III (DMBA, doxorubicin 1.12 mg/kg), IV (DMBA, fraction of 100 mg/kg), and V (DMBA, doxorubicin, fractions). All the rats were sacrificed at week 16. Their breast tissue was evacuated. Cell proliferation and expression was observed using AgNOR method and immunohistochemistry, respectively. Results showed the percentage of p-AgNOR obtained by the healthy group, DMBA group, DMBA+Doxorubicin group, DMBA+ethyl acetate fraction of E. longifolia Jack root group, and DMBA+Doxorubicin+Ethyl acetate fraction of E. longifolia group were 00%, 14.672.11%, 1.831.21%, 6.832.03%, and 4.080.95%, respectively. The percentage of Bax expression obtained by the healthy group, DMBA group, DMBA+Doxorubicin group, DMBA+ethyl acetate fraction of E. longifolia Jack root group, and DMBA+Doxorubicin+Ethyl acetate fraction of E. longifolia group were 68.821.52%, 26.863.25%, 44.492.06%, 80.923.27%, and 78.704.87%, respectively. Based on the results, it was concluded that co-chemotherapy agent of ethyl acetate fraction of E. longifolia Jack roots and Doxorubicin could inhibit proliferation and trigger apoptosis through Bax expression in breast tissue of rats induced by DMBA.
VALIDASI METODE KROMATOGRAFI LAPIS TIPIS (KLT)-DENSITOMETRI UNTUK PENETAPAN KADAR β-KAROTEN DALAM TABLET KUNYAH EKSTRAK Spirulina platensis Siti Fatmawati Fatimah; Citra Ariani Edityaningrum; Wihasty Nur Istyqomah; Ibnu Gholib Gandjar; Laela Hayu Nurani
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 5 No 1 (2020): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.6 KB) | DOI: 10.36387/jiis.v5i1.404

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Analytical Validation Method to determine β-carotene in the Spirulina platensis extract chewable tablets need to be done to ensure the quality. The purpose of this study is to prove the validity of the TLC-Densitometry method for determining the levels of β-carotene in the Spirulina platensis extract chewable tablets. Validation of the analysis method was carried out using standard β-carotene and spirulina chewable tablets in ethyl acetate solvents including specificity, linearity, repeatability precision, intermediate precision, limit of detection and limit of quantitation with stationary phase of silica gel 60 F254 and mobile phase of acetone: ethyl acetate (1: 1 v/v) using the TLC method, then quantitative analysis was carried out with a Densitometer at a wavelength of 453 nm. The results showed that the TLC-Densitometry method was linear with an r value of = 0.9938, having a repeatability precision with RSD 1.89%, intermediate precision with an RSD value of 2.401%, an LOD of 0.159 mg/mL and an LOQ of 0.531 mg/mL, and the specificity showed that this method is not specific to β-carotene compounds and the accuracy does not indicate a recovery value. Based on these results it can be concluded that the TLC-densitometry method is invalid for the determination of β-carotene levels in the Spirulina platensis extract chewable tablets.
EFEKTIVITAS KOMBINASI PLASMA JET NON -THERMAL DAN SPRAY Aloe vera (L). Burm. f . PADA PENYEMBUHAN LUKA DIABETES Ratih Arum Astuti; Laela Hayu Nurani; Eka Wahyuning Tyas; Iis Wahyuningsih; Devi Kumala Dewi; Isabella Meliawati Sikumbang; Nasruddin Nasruddin
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 6 No 2 (2021): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (599.87 KB) | DOI: 10.36387/jiis.v6i2.708

Abstract

Plasma jet non thermal has benefit in wound healing, but less control can to accumulation of reactive oxygen species. This study aims to determine the effectiveness of the combination of plasma jet and A. vera spray 1% for wound healing in diabetic wounds mice model with parameters of malondialdehyde levels, the skin wound ratio, and blood profile. This study used male Balb/C were induced by a streptozotocin (STZ) and divided into 4 groups, namely groups of untreated diabetic mice wounds (N), groups of diabetic mice wound with A. vera spray 1% (Av), groups of diabetic mice wound with plasma jet non thermal at distance of 20 mm for 3 minutes (P), and groups of diabetic mice wound with plasma jet non thermal at distance of 20 mm for 3 minutes and A. vera spray 1% (PAv). The solution of A. vera spray 1% preparation consists of A. vera, DMSO, and aquabidest extracts. The wounds of all groups treated for 14 days then the malondialdehyde, skin wound ratio and blood profile were calculated by SPSS Statistic. The results of blood glucose before treatment were 241.74 dL ± 51.99. The combination of plasma jet and A. vera spray 1% groups were significant (p <0.05) in reduce malondialdehyde levels, reduced the the skin wound ratio but not different significantly (p> 0.05), and not effect on blood profile data (p> 0.05). In conclusion, A. vera spray 1% can reduce the side effects of plasma jet in diabetic wounds by reducing malondialdehyde levels.
PENGARUH PEMBERIAN MINYAK BIJI JINTEN HITAM TERHADAP KADAR INTERLEUKIN-6 PADA PEROKOK AKTIF SEHAT Arif Santoso; Akrom Akrom; Laela Hayu Nuraini; Titiek Hidayati
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 7 No 1 (2022): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.385 KB) | DOI: 10.36387/jiis.v7i1.861

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Exposure to cigarette smoke causes cellular oxidative stress and is the key to the inflammatory process in the lungs. Black cumin seed oil (MBJH) is one of the traditional medicines that is proven to contain the active substances timoquinone, nigelon and negelin which have antioxidant and immunomodulatory effects. Interleukin-6 (IL-6) is a proinflammatory cytokine and a multifunctional cytokine that is very important for determining the immune response, cell survival, apoptosis and proliferation and is a protumorogenic agent in many cancers. The purpose of this study was to determine the levels of IL-6 in healthy active smokers after being given MBJH at a dose of 0.5 ml/capsule. The research design used was a single blind Randomized Controlled Trial (RCT). A total of 39 healthy healthy active smokers were divided into 4 groups: Group 1 was given a placebo 3x1 capsules/day, group 2 was given MBJH 3x1 capsules/day, group 3 was given MBJH 3x2 capsules/day and group 4 was given MBJH 3x3 capsules/day. The placebo and MBJH interventions were administered for 30 days. Statistical analysis obtained showed that there was no significant difference in the mean levels of IL-6 between the treatment group and the placebo group with p>0.05. The results showed that the use of MBJH for 30 days can reduce the average level of Interleukin-6 but not significantly in healthy active smokers.
Hubungan antara Konsentrasi Minyak Atsiri Kayu Manis (Cinnamomum burmannii Nees Ex Bl.) dalam Lotion dengan Sifat Fisik dan Tingkat Kesukaan Konsumen Erwan Kurnianto; Nining Sugihartini; Laela Hayu Nurani
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Volume 13 Nomor 1 Juni 2017
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1558.967 KB) | DOI: 10.22435/blb.v13i1.251

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Essential oil of Cinnamomum burmannii contains cinnamaldehyde as active substance as antibacterial and repellant. The study about development of dosage forms was needed in order be useful to people. The purpose of this study was to determine the concentration’s effect of cinnamon to the physical characteristic and results hedonic test of lotion. This study was done in Laboratory of Pharmacy UAD in 2014 that was begun with distillation of essential oil. Then, it was formulated as lotion with concentration 1% (F1), 3% (F II), 5% (F III), 7% (F IV), respectively. The lotion was evaluated its physical characteristic (homogeneity, viscosity, spreadability, adhesivity, pH) and hedonic test to know the level of acceptability based on colour and flavor of lotion. Data was collected by quistionnaire. Results of study showed that all of the lotion was homogenous. The increasing concentrations of cinnamon caused decreasing of viscosity (p <0.05) and adhesivity (P <0.05), an increasing of spreadability (P <0.05), did not affect the pH value (p> 0.05), and increasing hedonic level until 5% concentration. It can be concluded that concentration of essential oil of cinnamon at 5% fullfilled the requirement of spreadability, adhesivity, pH and the most prefered by consumers.
SKRINING KESEHATAN JIWA, RELIGIUSITAS, DAN KUALITAS HIDUP MAHASISWA FARMASI DAN KEDOKTERAN PADA PANDEMI COVID-19: SCREENING OF MENTAL HEALTH, RELIGIUSITY, AND QUALITY OF LIFE OF PHARMACEUTICAL AND MEDICAL STUDENTS IN THE COVID-19 PANDEMIC Widea Rossi Desvita; Wiwara Awisarita; Muhammad Fikri; Akrom; Laela Hayu Nurani; Isabella Meliawati Sikumbang
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 7 No 2 (2022)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v7i2.342

Abstract

Kesehatan mental mahasiswa Farmasi dan Kedokteran perlu diskrining terhadap keberadaan COVID-19. Faktor internal yang perlu diperhatikan adalah aspek religiusitas dan kualitas hidup. Instrumen yang dikembangkan untuk pemeriksaan kesehatan mental adalah SRQ29 serta religiusitas dan kualitas hidup dengan EQ5D. Tujuan dari penelitian ini adalah untuk mengetahui kesehatan mental dan kualitas hidup mahasiswa Farmasi dan Kedokteran pada masa pandemi COVID-19. Penelitian ini merupakan penelitian cross sectional diskriptif dengan metode survey terhadap 44 responden yang meliputi 36 mahasiswa Fakultas Farmasi dan 8 mahasiswa Fakultas Kedokteran. Metode yang digunakan untuk skrining kesehatan jiwa adalah instrumen kuesioner yaitu SRQ-29 untuk skrining kesehatan jiwa dan religiusitas yang berisi 29 pertanyaan terkait kesehatan jiwa responden, dan skrining kualitas hidup dengan instrumen EQ5D. Hasil penelitian menunjukkan bahwa dari skrining SRQ29 mahasiswa farmasi dan FK  perguruan tinggi di Yogyakarta mengalami gejala PTSD (75%), cemas dan depresi (61,36%), gangguan psikotik (50%), penggunaan zat psikoaktif/ narkoba (2,27%). Berdasarkan hasil skrining religiusitas menunjukkan nilai yang tinggi terhadap keyakinan dan iman kepada Allah SWT (96,8%). Hasil EQ5D menunjukkan bahwa pandemi tidak berdampak besar pada aspek fisik, namun berdampak pada mental/ psikologis. Kesimpulan penelitian terkait kesehatan jiwa pada hasil skrining dengan instrumen SRQ29 menunjukkan bahwa mahasiswa farmasi dan FK  perguruan tinggi di Yogyakarta mengalami masalah psikososial.  Hal ini saat dikaitkan dengan religiusitas dan kualitas hidup menunjukkan adanya pengaruh faktor internal tersebut pada kesehatan jiwa.
Peningkatan Pengetahuan Ibu dalam Pengolahan Tanaman Obat Keluarga (TOGA) sebagai Relaksan dan Skrining Kesehatan Jiwa Widea Rossi Desvita; Tri Ani Marwati; Fikri Achmad Arif; Isabella Meliawati Sikumbang; Laela Hayu Nurani
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 7 No Special-1 (2022): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v7iSpecial-1.2514

Abstract

The COVID-19 pandemic impacts maternal psychological problems with an increasing prevalence of symptoms of depression and anxiety. In Branch Aisyiyah Demangan and Sapen DIY, activities were stopped during the pandemic, considering that many of the members were elderly and were in the red zone in June 2021. On this basis, it is necessary to carry out stimulus activities that can be carried out during a pandemic. This activity aims to know mental health conditions by screening mental health, promoting mental health, and using and managing traditional medicines for relaxants. The methods used in this activity are mental health screening, mental health counseling and training, presentation of herbal medicines with the right dose, and making herbal medicine that meets the standards of the Health Office. Activities are carried out online, starting with pretest, delivery of material, mental health screening, management of traditional medicinal plants, post-test. The analysis of the results was carried out by performing a paired t-test statistical test. The pretest and post-test results showed an increase from 82±0.55 to 93±0.84. Psychologically and emotionally, most respondents experienced complaints of fatigue (73.1%), followed by complaints of difficulty understanding feelings (46.2%), then complaints of anxiety, tension, or worry (42.3%). Screening results show that one respondent has a mild mental problem.
Co-Authors Abdul Rohman Abdul Rohman Abdul Rohman Abdul Rohman Achmad Mursyidi Achmad Mursyidi Afiana Rohmani Agitohutomo, Muhammad Ainiyah, Nadia Putri Aisyah, Vani Aisyah, Vani Akrom Akrom, Akrom Aldri Frinaldi ANDI WIJAYA Anggita Rosiana Putri Anjar Windarsih Annisa Fatmawati Any Guntarti Any Guntarti Any Guntarti Any Guntarti Any Guntarti Arif Santoso Aris Asahi Aristiani, Windi Aristiani, Windy Astuti, Ratih Arum Azzahra, Fara Bachri, Mochammad Saiful Citra Ariani Edityaningrum, Citra Citra Dhea Cantika Danang Prasetyaning Amukti Daru Estiningsih Daud, Intan Devi Kumala Dewi Dewi Andini Kunti Mulangsri, Dewi Andini Kunti Dewi, Devi Kemala Dian Prasasti Djannah, Sitti Nur Djannah, Sitti Nur Dwi Utami Edityaningrum, Citra Ariyani Efiana, Nuri Ari Eka Kumalasari Eka Kumalasari Eka Kumalasari Eka Sakti Wahyuningtyas Eka Wahyuning Tyas Elya Zulfa, Elya Endang Darmawan Eti Nurwening Sholikhah Eti Nurwening Sholikhah Fara Azzahra Fara Azzahra Fauziyya, Riri Feri Anggita Hastanto Feri Anggita Hastanto Fikri Achmad Arif Fitriyati, Laeli Galih Dwi Mulyati Gela Setya Ayu Putri Hari Susanti Heni Lutfiyati, Heni Heni Setyowati Esti Rahayu Hidayati, Sholihatil Husnun Khairunnisa Pratiwi Ibnu Gholib Gandjar Ibnu Gholib Gandjar Ibnu Gholib Gandjar Ichsan Luqmana Indra Putra, Ichsan Luqmana Indra Ichwan Ridwan Rais Iin Narwanti Iin Narwanti Iis Wahyuningsih Intan Rahayu Irfan, Nawwar Irnawati Irnawati Isabella Meliawati Sikumbang Isabella Meliawati Sikumbang Jumina Jumina Jumina Jumina Kartikadewi, Arum Khairurrizki, Amanda Khayatulisma, Dika Kurnianto, Erwan Lalu Muhammad Irham Mahardika Agus Wijayanti Ma’ruf, Muhammad Meta Safitri Milanie, Rida Dwi Miratun Syarifah Moch Saiful Bachri Moch. Saiful Bachri Moch. Saiful Bachri Moch. Saiful Bachri Muhammad Fikri Muhammad Ma'ruf Muhammad Yusron Maulana El-Yunusi Mustofa Mustofa Nanik Sulistyani Nasruddin Nasruddin Nasruddin Nasruddin Nasruddin, N Nina Salamah Nining Sugihartini Nining Sugihartini Nugrahini, Febrina Nur Azizah Nur Ismiyati Nurkhasa Nurkhasa, Nurkhasa Nurkhasanah Nurkhasanah Mahfudh Nurkhasanah Nurkhasanah Putri Kurniasiwi Putri Lestari Rahma Dona Ramadhan, Muhammad Marwan Rini Sulistyawati Rizki Amelia Rohman, Abdul Salsabila, Nia Sapto Yuliani SATRIYAS ILYAS Sikumbang, Isabella Meliawati Sitarina Widyarini Sitarina Widyarini Siti Fatmawati Fatimah, Siti Fatmawati Siti Rofida Siti Salma Yusuf Siti Setianingsih Sitti Nur Djannah Sudjadi . Sugiyanto - Sulistyorini, Dwi Agustin Suwidjiyo Pramono Syarif, Yaumi Musfirah Titiek Suhardi Haripurnomo Kushadiwijaya Hidayati Tri Ani Marwati Tri Yanuarto Tria Zakinah Utari, Dina Vani Aisyah Vivi Sofia Wahyu Widyaningsih Wahyuningtyas, Nurma Widea Rossi Desvita Widyarini, Sitarina Wihasty Nur Istyqomah Wiwara Awisarita Yaumi Musfirah Syarif Yeni Dianita Yeni Yeni, Yeni Dianita Yeni, Yeni Dianita Yoga Yuniadi Yusuf, Siti Salma Zainab Zainab Zainab Zainab