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Nurhaida Nurhaida
Fakultas Ekonomi Universitas Syiah Kuala
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Journal : Jurnal Natural

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The validation of urea determination in saliva using optical urea biosensor with p-dimethylaminobenzaldehyde (DMAB) reagent ALDIANSYAH, TEUKU; SAFITRI, EKA; GINTING, BINAWATI; NAZARUDDIN, NAZARUDDIN; SUHUD, KHAIRI; SALEHA, SITTI; NURHAYATI, NURHAYATI; NAZARUDDIN, SALSABILLA LATANSA; AFIFI, MUHAMMAD RIDHO; NURHAIDA, NURHAIDA
Jurnal Natural Volume 25 Number 1, February 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v25i1.41251

Abstract

The validation of urea determination in saliva using the biosensor method and the p-dimethylaminobenzaldehyde (DMAB) method has been successfully conducted. Anthocyanin compounds from Chatarantus roseus flowers were extracted using the maceration method with methanol as the solvent, yielding a total of 22.60% with a total concentration of 6.01 mg/L. The obtained extract was subjected to qualitative anthocyanin testing, showing a positive result was indicated by the formation of a reddish color and quantitative testing with the formation of a faded yellow color. The maximum wavelength ( max) of anthocyanin was 664 nm with an absorbance of 0.674. The saliva samples were obtained from three different ages i.e: children, teenagers, and adults. During the saliva collection procedure, participants were instructed to abstain from consuming any food or beverages, with the exception of water, for a minimum of 1 hour prior to sample collection. All samples were stored in a freezer at 4-8C until needed for analysis. Linearity test results were assessed using a calibration curve, yielding a coefficient of determination (R) of 0.978 with a sensitivity of 0.033 for the biosensor and an R of 0.975 with a sensitivity of 0.685 for DMAB. The limit of detection (LOD) values were determined as 7.203 10 M for the biosensor and 6.984 10 M for DMAB, while the limit of quantification (LOQ) values were 2.182 10 M and 2.116 10 M, respectively. Statistical analysis using a t-test showed tcalculated = 1.314, compared to ttable = 4.302, indicating that tcalculated ttable. This result suggests no statistically significant difference between the biosensor and DMAB methods, confirming that the biosensor method is comparable in performance to the DMAB method. Additionally, urea concentration measurements showed that adult samples exhibited the highest urea levels among the tested samples, which may indicate an association with potential health risks, including dental caries, kidney failure, and liver damage.
Phytochemical screening and antibacterial activity testing of n-hexane extract from Barringtonia asiatica seeds NURHAIDA, NURHAIDA; MURNIANA, MURNIANA; ATANTA, JAINURSUMARMI SAFRULLAH
Jurnal Natural Volume 24 Number 1, February 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v24i1.32597

Abstract

Barringtonia asiatica, a marine plant belonging to the Lecythidaceae family, exhibits bioactivity in all parts of the plant. In the Simeulue region, the seeds of this plant are used for fish trapping. The polar extracts of B. asiatica seeds has shown antibacterial activity, but the non-polar extracts has not been studied yet. Therefore, this study aimed to determine the secondary metabolites of B. asiatica using a non-polar solvent (n-hexane) and evaluate its activity against Gram-positive and Gram-negative bacteria. The extraction method employed using soxhlet extraction, and the antibacterial activity was determined using the Kirby-Bauer method. The presence of secondary metabolites in the B. asiatica seed extract was assessed using phytochemical tests, and its chemical composition was analyzed using Gas Chromatography Mass Spectrophotometry (GC-MS). The yield of n-hexane extract from B. asiatica seeds was 1.7%, and the phytochemical analysis revealed that the B. asiatica seeds n-hexane extract contains only terpenoids compounds. The B. asistica seeds n-hexane extract at concentration of 10% (w/v) showed the highest zone of inhibition against Staphylococcus aureus (9.3 mm) followed by Escherichia coli (9,9 mm). The GC-MS analysis showed that B. asiatica seeds n-hexane extract contains Guaiene, Guaiene, Ledene, 9,12-Octadecadienoic acid, and Ethyl linoleate. Based on these findings, it can be concluded that B. asiatica seed's n-hexane extract exhibited antibacterial activity against S. aureus and E. coli.
Co-Authors Abd. Rasyid Syamsuri Afifi, Muhammad Ridho ahmad yani Ainun Mardhiah, Ainun Ainun Mardiah Alam, Samsu ALDIANSYAH, TEUKU Amrina, Bella Septie andi, adi Anwari, M Sofwan Apriani, Riny Aprilyani, Lilis Dwi Sapta ARDIANSYAH ARDIANSYAH Aritonang, Devinna Riskiana Astuti, Tani ATANTA, JAINURSUMARMI SAFRULLAH Aulia, Astrina Azfa, Naila Arifah Azzahra, Mayochi Binawati Ginting Cut Aja Nuraskin Darwanti, Herlina Dewi, Shoufi Nisma Diana Diana Dina Setyawati Dirhamsyah, Muhammad Eka Safitri Elvi Susanti Lubis Farah Diba Febriani, Henny Femi Earnestly Firdaus Firdaus Fitri Kurnia Fitriani Fitriani Ginting, Bersiap Gitriadi, Heru Gusti Hardiansyah Gustina, Jessi Gustira, Ndari Habibi Habibi Hari Prayogo Hasan Ashari Oramahi Havija Sihotang Hutauruk, Puput Melati Indah Susanti Indrawasih, Indrawasih Isnaini, Nur Uli Khairunisa, Ana Laode Muhammad Harjoni Kilowasid Leli Suwita, SE, MM Lestari, Yayuk M. Dirhamsyah Maharani, Dhevia Marwanto Marwanto, Marwanto Matulessy, Esther Ria Muchlisinalahuddin Mufizarni, Mufizarni Muflihati, . Munadian, Munadian Murniana Murniana Muslim, Fluorina Oyza Mutia, Hera Mutiar, Mutiar N. Nazaruddin Nadia Nadia Najwa Najwa Nanang Masruchin Nasution, Putri Nabila NAZARUDDIN, SALSABILLA LATANSA Nengcy, Silvia Nova Rita, Nova Nugraha, Rezky Adrian Nurhasyifa, Najwa Nurhayati Nurhayati Nurhidayah, Kurnia Nursabila, Najwa Oktavia, Elmi Oktaviani, Maria Gerlina Peterson, Peterson Prita Larasati, Prita Larasati Puguh Setiawan Purwano, Sigit Putri, Puji Pradya R, Siti Raftul Fedri Rahayu, Eka Sri Rangkuti, Miftahul Nur Khairi Ranglaut, Sebastianus Lio Rago Rasyid, Syarifah AB Reca Reca Refiadinata, Jeki Refialdinata, Jeki Ria Matulessy, Esther Rikandi, Meta Riko, Eryesiswanto Sabyan, Muhammad Salihin, Salihin Santoso, Ida Riyanti Saputra, Muhammad Romi Saputri, Melda SARAWA, SARAWA Sarma Siahaan Sesa, Junianto Sinaga, Patresia Siregar, Alfin Siregar, Liesma Maywarni Sitti Saleha, Sitti Sofyan Zainal Sri Wahyuni Suhud, Khairi Suri, Mellan Suryani, Cici Susanti, Baiq Indah Susanti, Yana Yetria Syahfira A Syahna Syahna, Syahfira Ananda Teuku Salfiyadi Trisna Wati, Trisna Usmiar, Usmiar Wahdina, Wahdina Wahyuni, Dian Eka Mayasari Sri Widiyanti, Rina Widyanti, Rina Widyawati Widyawati Wijayanti, Riana Wirawan, Hadi Purnama Wirza Wirza, Wirza Wulandari, Reine Suci Wulandari Yuliaty, Ihdina Yundelfa, Mandria Zulfahmi Zulfahmi