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All Journal Media Veteriner Jurnal Veteriner Jurnal Biodjati Jurnal Medik Veteriner Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology Indian Journal of Forensic Medicine & Toxicology journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Jurnal Pengabdian Masyarakat Charitas Jurnal Agro Veteriner

Pudji Srianto

Department Of Veterinary Reproduction, Faculty Of Veterinary Medicine, Universitas Airlangga

Author-ID : 3693346

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Engineering Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Social Sciences Veterinary

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PERSENTASE KEJADIAN KAWIN BERULANG SAPI PERAH YANG TELAH DI IB (INSEMINASI BUATAN) TINGKAT PETERNAK DESA NYAWANG KIDUL DI KUD TANI WILIS KECAMATAN SENDANG KABUPATEN TULUNGAGUNG Setyadi, Arda Wahyu; Srianto, Pudji; Widodo, Oky Setyo; Wurlina, Wurlina; Samik, Abdul; Restiadi, Tjuk Imam
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Dairy cows farm business level of KUD Tani Wilis in Nyawang Kidul, Sendang district, Tulungagung city has some problems resulting low livestock productivity. One of the causes of low levels of dairy reproductivity is the incidence of repeat breeder. Repeat breeder is a condition of a female cow that failed to breed after being mated three or more times with a normal and healthy fertilized bull. The main purpose of this research is to know the risk factor of repeat breeder in dairy cows at farmer level of KUD Tani Wilis in nyawang kidul, Sendang district, Tulungagung city. The type of research is descriptive research. This research was using 32 dairy cows from 6 breeders, the selection of cattles used Simple Random Sampling method. The collection data using questionnaires and direct observations in farms resulted that the evidence rate of dairy cattle's repeat breeder in this research is 45.7%. The results also showed that the influence of knowledge of farmers on disease or reproductive disorders positively correlated with the evidence of repeat breader.

Kualitas spermatozoa post-thawing pejantan sapi Friesian Holstein pada umur yang berbeda Ristiani, Widya Aulia; Yunus, Muhammad; Suprayogi, Tri Wahyu; Srianto, Pudji; Mustofa, Imam; Rimayanti, Rimayanti
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This study aimed to determine the post-thawing spermatozoa quality of Holstein-Friesian (HF) bulls at different ages. The samples were HF frozen semen of 3, 5, and 8-years -old age group produced by the Singosari National Artificial Insemination Center. Nine samples of each group were randomly purposive sampling, thawed in 37ºC water for 15 seconds, and assessed on the percentage of viability, motility, and abnormality of spermatozoa. The obtained data were analyzed using the ANOVA method, followed by Duncan's multiple range test at a 95% confidence level. The result showed that post-thawing spermatozoa viability in 5 and 8-year-old HF bull were 67 ± 6.67%, and 78 ± 7.59, both were higher (p <0.05) compared to 62.78 ± 5.76% in HF bulls 3-yearsold group. Likewise, the motility of those bull aged 5 and 8 years (69.44 ± 6.82 and 65.00 ± 9.68%, respectively) were higher (p <0.05) compared to 55, 56 ± 5.83% in 3-year-old HF bull. Meanwhile, the percentage of post-thawing spermatozoa abnormalities in the three age groups were 4.33 ± 1.96%, 5.89 ± 1.90%, and 5.33 ± 1.41%, not significantly different (p> 0.05) respectively. It concluded that the best quality of post-thawing HF semen based on the percentage of viability, motility, and morphologic abnormality was collected from 5-8 years old bull. Nevertheless, spermatozoa from the three age goups still meet the Indonesia standard quality of frozen semen for artificial insemination.

Angka kebuntingan sapi perah dengan korpus luteum persisten setelah pemberian kombinasi prostaglandin F2α dan gonadotropin Azwani, Nabila; Srianto, Pudji; Madyawati, Sri Pantja; Sardjito, Trilas; Suprihati, Endang
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 2 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i2.2021.59-64

This case study aims to determine the effect of a combination of prostaglandin F2α (PGF2α) with Gonadotropin (PG-600), and human chorionic gonadotropin (hCG) on the estrus and pregnancy rates in Holstein Friesian (HF) cows with persistent corpus luteum (PCL). A total of 15 Holstein Friesian (HF) dairy cows aged 3-5 years with body condition score (BCS) 5-7 (scale 1-9) diagnosed with PCL, were injected with 7.5 mg PGF2α intra-vulvar submucosa. Cows were then divided into three treatment groups. Group P0 was not given the combination treatment, while P1 group was injected with a combination of 300 IU PG-600 and 150 IU hCG, meanwhile P2 group was was injected with a combination of 300 IU PG-600 and 300 IU hCG intra-muscularly. The PGF2α and PG-600 injections were carried out on the same day PCL was diagnosed. Meanwhile, the injection of hCG was carried out at the same time as the AI. The Pregnancy examination was performed 90 days after AI through rectal palpation. The results showed that all treated cows were estrus observed with the fastest onset of estrus was the P2 group, that was significantly different (p <0.05) compared to the P0 and P1 groups. All treated cows were diagnosed as pregnant. It can be concluded that the administration of PGF2α or its combination with gonadotropins in HF cows with PCL could result in 100% estrus and pregnancy rates.

Kualitas semen sapi pejantan berdasarkan umur, suhu, dan kelembaban di Taman Ternak Pendidikan Universitas Airlangga Brillianti, Febby Fairy; Srianto, Pudji; Sardjito, Trilas; Suprayogi, Tri Wahyu; Triana, Indah Norma; Rahardjo, Dadik
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 3 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i3.2021.81-89

This research was conducted in the teaching farm of the Faculty of Veterinary Medicine, Universitas Airlangga. The present study aimed to determine the effect of age, temperature, and humidity on bull semen quality which was processed into frozen semen. Three age groups of bulls (young 3-4 years, middle age 5-6 years, and old 7-8 years) were collected for the temperature and humidity of semen examination. Fresh semen was collected using an artificial vagina, followed by macroscopic and microscopic examination. In addition, post-thawing motility was also evaluated. The results showed that there was no effect of temperature and humidity during semen collection process. Fresh semen quality was affected by age. Moreover, the post-thawing motility of fresh semen of spermatozoa was lower than those of the fresh semen; however, it still qualifies for artificial insemination. It could be concluded that the semen of bulls at the Teaching Farm of Universitas Airlangga has good quality, and is suitable for Artificial Insemination.

Determination of sexual maturity of Indonesian box turtle (Cuora amboinensis couro) based on straight carapace length Daniel Leonardo; Pudji Srianto; Iwan Sahrial Hamid; Boedi Setiawan; Djoko Legowo; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.115-122

Predicting the turtle's reproductive status and sexual maturity is necessary for ex-situ breeding and conservation program. The histology of the seminiferous tubules of the Indonesian box turtle (Cuora amboinensis couro) at various ages has not been studied much. This study aims to develop equations to predict the sexual maturity of Indonesian box turtles based on straight carapace length (SCL). Six turtles with SCL sizes 10-21 cm were sacrificed and their testes were taken for morphometric measurements and histological preparations for Hematoxylin Eosin staining. Pearson's correlation of SCL with testicular maturity parameters was analyzed, followed by Anova Regression. The results showed that there were differences in the morphometry and topographic anatomy of the Indonesian box turtle testes between SCL below 15 cm and above 15 cm. Six turtles with SCL size of 10-21 cm were sacrificed and their testes were taken for morphometric measurements and histological preparations for Hematoxylin Eosin staining. Pearson's correlation of SCL with testicular maturity parameters was analyzed, followed by Regression Anova. The results showed that there were differences in the morphometry and topographic anatomy of the Indonesian box turtle testes between SCL below 15 cm and above 15 cm.

Reproduction record of captive Sumatera elephant (Elephas maximus sumatranus) at Way Kambas National Park, Indonesia Sofie Adi, Keren Jayanti; Budiarto Budiarto; Hermin Ratnani; Pudji Srianto; Budi Utomo; Suzanita Utama; Setiawan Koesdarto; Nurhusien Yimer
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 3 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i3.2023.157-165

This research aims to determine reproductive data on Sumatran elephants (Elephas maximus sumatranus) in the ETC and ERU of Way Kambas National Park, Indonesia during 1988-2021. Data recorded from the elephant population at both locations (ETC and ERU) includes the number, gender of elephants, elephant calves, ages and birth dates. Calving intervals and service periods were calculated from calving records of cows with a minimum parity of two. Data collection produced data on 47 elephant calves from 13 female elephants with at least twice the parity and 12 primiparous cows. Elephant reproductive records at ETC and ERU were: age (37.44 ± 9.03 vs. 29.75 ± 3.30 years), parity (2.78 ± 1.09 vs. 2.50 ± 0.58), and age at first birth (18, 11 ± 3.92 vs. 17.75 ± 0.50 years). While the calving interval was 1857.56 ± 870.81 vs. 1833.00 ± 305.18 days, and service period respectively 1229.44 ± 846.18 vs. 1210.50 ± 283.59 days, respectively. It can be concluded that the calving interval and service period for captive elephants at ETC and ERU were not much different and were within the normal range. Young cows showed better reproductive efficiency than older cows. Monitoring calves is very important. Further assistance is needed to improve elephant mobile veterinary services, increase diagnostic laboratory capacity, and educate camp managers, veterinary assistants, and mahouts about elephant diseases, their monitoring and treatment. In addition, the use of reproductive technology such as monitoring ovulation using ultrasound and carrying out artificial insemination was expected to increase reproductive efficiency.

THE EFFECT OF UREA SUPLEMENTATION IN MATURATION MEDIA OF BOVINE OOCYTE IN VITRO TOWARDS EXPRESSION OF BAX, BCL-2 AND BAX/BCL-2 RATIO Kartikasari, Dhesy; Mulyati, Sri; Utama, Suzanita; srianto, Pudji; Widjiati, Widjiati; Plumeriastuti, Hani
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i1.15283

This study aims to evaluate the expression of BAX, BCL-2, and BAX/BCL-2 ratio in maturation media of cow oocytes which supplemented with Urea in vitro because BAX and BCL-2 are the main regulators of apoptosis.A total of 263 oocytes from follicle aspirations originating from ovaries taken from slaughterhouses and were saturated with 3 addition of urea which was divided into three groups. The control group (P0) was control group without the addition of urea, P1 group was added with urea 20 mg/dL, while P2 group was added with urea 40 mg/dL.The results of in vitro oocyte maturation were continued with identification using immunocytochemical staining with the addition of BAX and BCL-2 antibodies.Positive results showed a brownish color on the oocyte and its cumulus.The results of this study indicated that there were significant differences (P0.05) in BAX and BCL-2 expression, although both curves were equally increase. The increase in BCL-2 was more significant than BAX, while the BAX BCL-2 ratio did not show a significant difference (P0.05) in whichthe curve of BAX/BCL-2 ratio was decreased.It can be concluded that the addition of urea does not affect the level of apoptosis.

Co-Authors . Kholik Abdul Samik, Abdul Abdullah Hasib Aditya Irawan Aftabuddin Rz, Muhammad Agustono, Bodhi Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Aldin Akbar Rahmatullah Ali Saifudin Andriyanti, Silvia Rani Azwani, Nabila Bambang Poernomo Berliana, Erprinanda Galuh Bhaskara, Alfiutha Propha Boedi Setiawan Brillianti, Febby Fairy BUDI UTOMO Budi Utomo Budiarto Budiarto Budiarto Cahya P., Aghid Cindy Ercha Aulia Putri Dadik Rahardjo Dadik Rahardjo, Dadik Dadik Raharjo Dadik Raharjo Daniel Leonardo Darsono, Roesno Deskayanti, Asih Dhesy Kartikasari Dian Ayu Permatasari Dian Ayu Permatasari Dikman, Dicky Mohammad Dimas Kunto Satrio Djoko Legowo Dwi Puspa Sukma Viranda Emy Koestanti Sabdoningrum Endang Suprihati Erlangga, Made Bagus Erma Safitri Erma Safitri ERMA SAFITRI Fajar Ramadhan Subiyantoro Febriano, Muhammad Galang Gandul Atik Yuliani Hani Plumeriastuti Hariadi, Mas'ud Heni Puspitasari Hermin Ratnani Herry Agoes Hermadi Imam Mustofa IMAM MUSTOFA Indah Norma Triana, Indah Norma Ira Sari Yudaniayanti Ismudiono Ismudiono Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Kartikasari, Dhesy Kurniawan, Muhammad ‘Ahdi Laba Mahaputra Lailiyah, Fahlevi Luqman, Epy Muhammad Masyitoh, Herlina Mohammad Auzaie Afandi Muhammad Aulia Rahman Muhammad Munawaroh Muhammad Rama Imam Saputra Muhammad Yunus M’ Izi Kumala Lazuardi Sultoni Nidom, Chairul Anwar Nove Hidajati Nurhusien Yimer Nuurin Ajrin Karim Praja, Ratih Novita Prastiya, Ragil Angga Pratama, Hanif Sabekti Pristian Devon Martyas Wahyuningrum Rahmawati Rahmawati Rahmawati, Mitha Ardila Rimayanti Rimayanti Rimayanti Rimayanti Rimayanti Ristiani, Widya Aulia Rizki, Andaru Rochmah Kurnijasanti Saputro, Amung Logam Seagames Waluyo, Seagames Setiawan Koesdarto Setyadi, Arda Wahyu Sintya Kumalasari Wibowo Sintya Kumalasari Wibowo Soeharsono Soeharsono Soeharsono Soeharsono Sofie Adi, Keren Jayanti Sri Hidanah Sri Mulyati Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Subagio, Muharam Suherni Susilowati Sukma, Puspa Permata Sunarso, Agus Supriyadi Supriyadi Suwarno Suwarno Suzanita Utama Tasya Apritalia Putri Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Viski Fitri Hendrawan Warsito, Sunaryo Hadi Widjiati w Widjiati Widjiati Widjiati Widjiati, Widjiati Widodo, Oky Setyo Widya Paramita Lokapirnasari Wulandari, Shelly Wurlina, W Yustinasari, Lita Rakhma Zahrorona, Amira Halimah

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