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All Journal Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Bioscientist : Jurnal Ilmiah Biologi Jurnal Agro Veteriner

Tjuk Imam Restiadi

Departemen Reproduksi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga

Author-ID : 3693364

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Detection of Fertility Levels of Female Bawean Deer (Axis kuhlii) Based on Fecal Steroid Metabolic Rahmawati, Mitha Ardila; Hariadi, Mas'ud; Restiadi, Tjuk Imam; Rimayanti, Rimayanti; Lestari, Tita Damayanti; Srianto, Pudji
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.84-90

The aim of the study was to detect the fertility level of females Bawean deer (Axis kuhlii) through fecal steroid metabolic which were tested using Enzyme-Linked Immunosorbent Assay (ELISA). The study used five females Bawean deer in Taman Flora Surabaya, which were marked using R1, R2, R3, R4, and R5 necklaces. The feces of each deer was collected on the 1st day, 6th day, 11th day, 16th day, and 21st day. The samples which were collected then extracted using the freeze dry method. Samples were tested using ELISA. The results of the measurement of the levels of fecal steroid metabolic in five Bawean deers showed that three Bawean deer (R1, R4, and R5) were in the luteal or pregnant phase as indicated by the increased of progesterone from 1st day to 21st day. While the other two Bawean deer (R2 and R3) were in the follicular or estrous phase as indicated by the decreased of level of faecal steroid metabolic on 11th day for deer R2 and 16th day for deer R3. The results showed that the levels of the fecal steroid metabolic can be used to determine the follicular phase and luteal phase and the pregnancy status of female Bawean deer.

Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences Amrullah, Muhammad Fajar; Utomo, Budi; Utama, Suzanita; Suprayogi, Tri Wahyu; Lestari, Tita Damayanti; Restiadi, Tjuk Imam; Belgania, Ristaqul Husna
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.125-131

The Leptin gene is the gene that produces the leptin hormone, which is released from adipose tissue and can increase the productivity of animals. This study aimed to identify polymorphic nucleotides, changes in amino acid components, and species of goats based on GenBank Leptin DNA sequence data. A total of five goat leptin DNA sequences were extracted from NCBI GenBank data. The leptin DNA sequence was aligned with Bioedit to locate SNPs and amino acid changes. The tree produces cultivars grown using Clustal Omega Ver. 1.2.4. Based on the DNA sequencing results of leptin genes in five goats, five SNPs were located in the coding sequence (CDS), SNPs g.17T/A, g.43T/A, g.74G/A, g.93C/A. and d. 386A/G. SNP was a missense mutation and a silent mutation. The analysis of phylogenetic trees of Leptin showed that there were three breeds of goats in one branch and two breeds of goats in different branches. These results provided the first report for further studies on the genetic diversity of leptin genes in different local goat breeds.

The Difference Of Feed Consumption, Body Weight, And Feed Conversation Ratio Between Male Muscovy Duck (Cairina moschata) And Male Mule Duck At The Growing Age Restiadi, Tjuk Imam
VITEK : Bidang Kedokteran Hewan Vol. 13 No. 2 (2023): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v13i2.268

This study aims to collect data on comparisons of the differences in body size between Muscovy Duck and Mule Duck that can be observed from feed consumption, weight gain and feed conversion ratio between Cairina moschata and Mule Duck to find out the best waterfowl as a cut poultry. Cairina moschata and Mule Duck that are maintained at the age of the growth phase that is from the age of 5 weeks to 9 weeks with gender male Cairina moschata and male Mule Duck were used in this study. The number of samples that were used was 10 Cairina moschata and 10 Mule Duck. The results of the data processing of this study used a t test method in SPSS. The results of this study were the consumption of Mule Duck feed is much more than Muscovy Duck, the weight gain of Mule Duck is much more than Muscovy Duck, and Mule Duck have a lower feed conversion ratio compared to the Muscovy Duck. This experiment concluded that Mule Duck performance are more superior compared to Muscovy Duck.

The addition of duck egg yolk in skim milk – fructose extender maintains sperm motility and viability of Muscovy duck (Cairina moschata) at 5°C storage Isti Annisa Ramahtia; Tatik Hernawati; Tri Wahyu Suprayogi; Tjuk Imam Restiadi; Djoko Legowo; Samuel Inioluwa Akeju
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.1-8

This study aimed to determine the addition of duck egg yolk in skim milk – fructose extender on sperm motility and viability of Muscovy duck (Cairina moschata) at 5°C storage. Ejaculates of Muscovy duck were divided into four portions equally. The first portion was for the control group (diluted in skim milk – fructose extender), and the second, third, and fourth portions were for the treatment groups of T1, T2, and T3 (diluted in skim milk – fructose extender added with 2.5, 5.0, and 7.5% v/v duck egg yolk). Sperm motility and viability were evaluated two hourly until the motility percentage reached 40%. The data were analyzed using ANOVA and Duncan test. The result showed that the highest percentage of spermatozoa motility and viability (p<0.05) was in the T2 group compared to the other groups. It could be concluded that the Muscovy duck semen extended in skim milk - fructose with 2.5 to 7.5% egg yolk of duck qualified for artificial insemination at storage up to 10 hours in 5°C storage.

Reproductive disorders of cows in several villages of Kedamean district, Gresik regency, East Java, Indonesia in 2023 Utomo, Budi; Rimayanti, Rimayanti; Restiadi, Tjuk Imam; Amrullah, Muhammad Fajar
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.18-29

Reproductive disorders are the main cause of economic losses in beef herds. This study aimed to determine the type of reproductive disorder in beef cows in Kedamean district, Gresik regency, related to feeding, age, and parity. The method used for this study was a survey. Primary data was obtained from field surveys by interviewing farmers, animal health officials, inseminators, and direct inspection of cows and pens. Secondary data was obtained from the Gresik Animal Husbandry and Animal Health Service. The data obtained was then compiled and performed descriptively. The results showed that the number of productive cows in this study area was 935, of which 340 (36.36%) had reproductive disorders. The highest type of reproductive disorder was ovarian hypofunction (OHF) (14.55%, 136/935) and the smallest was cystic ovaries (0.11%, 1/935). Cows aged two and three years (heifers and first parity) have a percentage of reproductive disorders of more than 60% compared to cows of other ages, most of them (13.82% and 12.65%) were OHF. The cows fed straw have a greater percentage of reproductive disorders than cows fed field grass. Cases of OHF engaged the first position followed by cases of silent estrus and persistent corpus luteum. It could be concluded that reproductive disorders in the form of OHF, persistent corpus luteum, and silent estrus in several villages in Kedamean district, Gresik regency, East Java, Indonesia in 2023 mostly occured at the age of two to three years on heifers or first parity, fed straw.

CONCEPTION RATE DAN SERVICE PER CONCEPTION PADA SAPI PERAH AKSEPTOR INSEMINASI BUATAN DI KUD ARGOPURO KECAMATAN KRUCIL KABUPATEN PROBOLINGGO Yohana, Nadia; Samik, Abdul; Aksono, Bimo; Sardjito, Trilas; Hermadi, Herry Agoes; Restiadi, Tjuk Imam
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Reproduction is one of essential factor directly affecting profitability in a dairy production system. Some of the reproductive factors that influenced to the developing of dairy cattle of small holder's farmer are Conception Rate (CR) Service per Conception (S/C). The study was conducted to know the CR and S/C of Friesian Holstein in KUD Argopuro, Probolinggo. The data is taken primary and secondary data. Primary data retrieval is done by direct observation, which includes several variables, where the variables include : the identity of the breeder, cages, and feeding and drinking. As for the secondary data obtained by recording the card Artificial Insemination (AI) owned by Inseminator in KUD Argopuro. The data which by collected from this sample are CR and S/C. This research was a case study. The materials used cattle as the acceptors were 10% from 728 lactation cattle. Data from the results of this research on CR (30%) and S/C (2,49). The conclusion of this research for CR the result were lower than normal and S/C the result were higher than normal.

PENGARUH PEMBERIAN INSULIN-LIKE GROWTH FACTOR-I (IGF-I) DARI SERUM KUDA CROSSBREED BUNTING TERHADAP FOLIKULOGENESIS MENCIT (Mus musculus) Abdullah, Abdullah; Restiadi, Tjuk Imam; Lastuti, Nunuk Dyah Retno; Damayanti, Tita; wurlina, wurlina; Safitri, Erma
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of the research was to know the effect of Insulin-Like Growth Factor-I (IGF-I) derived from pregnant crossbreed mare serum (PMS) in mice (Mus musculus) folliculogenesis. The subject of this research were 20 female mice. The research was arranged by Completely Randomized Design (CRD) with four treatments and five replications. The treatment were K0 = 10 ng/ml of physiological NaCl, P1 = 10 ng/ml of IGF-I PMS, P2 = 20 ng/ml of IGF-I PMS, and P3 = 40 ng/ml of IGF-I PMS. Observed variables are number of primary, secondary, tertiary and de Graff follicles. During the treatment the estrus cycle was also observed. The data of follicles number were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS significantly affect (p<0,05) on increasing of the primary and secondary follicles number. The addition of IGF-I PMS 20 ng/ml and 40 ng/ml can increase the primary and secondary follicle significantly (p<0,05).

UJI BERBAGAI ALAT DETEKSI BIRAHI TERHADAP ANGKA KEBUNTINGAN PADA SAPI PERAH DI KOPERASI TUNAS SETIA BARU KABUPATEN PASURUAN Andriyanti, Silvia Rani; Hariadi, Mas'ud; Darsono, Roesno; Srianto, Pudji; wurlina, wurlina; Restiadi, Tjuk Imam
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This research aims to determine the use of various estrus detection devices before artificial insemination as measured by pregnancy rate. The researcher used a sample of 60 cows were used in this research which is divided into four groups as a control and treatment groups (Thermopin®, Hauptner and Draminski groups), and each cow was detected for the sign of estrus by three devices that were Thermopin®, Hauptner and Draminski prion when doing an artificial insemination. The cows were observed to 45 days after artificial insemination for observing pregnancy. This research method is the exploration scope to prove the effect of various estrus detection tool against pregnancy rates. The result of ANOVA test, there is the effect of using a various estrus detection devices against pregnancy rates. Samples tested by Draminski with average (1.53±0.51) resulted in a 53,3% pregnancy rate, the samples were tested with Hauptner with average (1.67±0,48) resulted in pregnancy rate 66,7%. Without treatment or control, sample with an average of (1.67±0,48) resulted in a 73,3% pregnancy rate. While samples were tested with Thermopin® with average (2.00±0,00) resulted in a 100% pregnancy rate. In conclusion, Thermopin® the best detection tool than other estrus detection devices.

EFISIENSI REPRODUKSI SAPI PERANAKAN LIMOUSIN AKSEPTOR INSEMINASI BUATAN DI KECAMATAN TIKUNG, KABUPATEN LAMONGAN TAHUN 2016 Prastyorini, Vina Ari; Supranianondo, Koesnoto; Safitri, Erma; Restiadi, Tjuk Imam; wurlina, wurlina; Hernawati, Tatik
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this study was known the difference of reproductive performans Limousine Cross Breed that haved artificial insemination from diffrence straw from BBIB Singosari and BBIB Lembang covering Conception Rate, Service per Conception, and Calving Rate in period 2016th. This study uses survey research which data from primary and secondary. Primary data were obtained from interview with Limousine Cross Breed breeders and secondary data were obtained from artificial insemination officer's record. The observed variables are Conception rate (CR), Service per conception (S/C) and Calving rate (CvR). Data were analyzed descriptive that average and Chi Square.Result of research showed no significant difference (p>0.05) on CR, S/C and CvR between Limousin cross breed cows that haved artificial insemination from BBIB Singosari and BIB Lembang.

PENGARUH PEMBERIAN JAMUR Fusarium graminearum TERHADAP HISTOPATOLOGI TUBULUS SEMINIFERUS MENCIT (Mus musculus) Kabayo, Fierda; Samik, Abdul; Ismudiono, Ismudiono; Restiadi, Tjuk Imam; Soeharsono, Soeharsono; Plumeriastuti, Hani
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The aim of this research was to show the influence of seminiferous tubules histopathology of mice (Mus musculus) caused Fusarium graminearumexposure. This research was done in April-May 2017 in Microbilogy Laboratory Faculty of Science and Technology, Animal Laboratory, and Microbilogy Laboratory Faculty of Veterinary Medicine UniversitasAirlangga. This research used 20 male mice (Mus musculus) aged 6 weeks with 18-30 gram body weight. The mice devided into four groups: P0 given 0,25 ml Sodium chloride without Fusarium graminearum exposure by oral; P1 given 0,25 ml Fusarium graminearum exposure with dilution 102 by oral; P2 given 0,25 ml Fusarium graminearum exposure with dilution 103 by oral; and P3 given 0,25 ml Fusarium graminearum exposure with dilution 104 by oral. This treatment carried out for 21 days. Each milliliter dilution containing 228x106 spore for P1, 228x107 spore for P2, and 228x108 spore for P3. Then do the surgery and harvesting the testes then performed histopathological examination by scoring of seminiferous tubules. For data analyzing used non parametric difference Kruskall-Wallis and continued with Mann-Whitney. The result of this research was showed that decreased the spermatogenic cells in the process of spermatogenesis significant.

Co-Authors Abdul Samik, Abdul Abdullah Abdullah Agus Wijaya Agustono, Bodhi Amrullah, Muhammad Fajar Ananda, Hanifah Muslimah Andriyanti, Silvia Rani Bambang Hermanto Belgania, Ristaqul Husna Bimo Aksono Boedi Setiawan Budi Utomo Chusniati, Sri Dadik Rahardjo, Dadik Damayanti, Tita Darsono, Roesno Dian Ayu Permatasari Diba, Lucky Dinul, Ahmad Rajul Djoko Legowo DWI WIJAYANTI Endang Setyaningsih Endang Suprihati Erma Safitri Faisal Fikri Haditanojo, Wiyanto Hana Eliyani Hani Plumeriastuti Hanifah, Novi Fitria Hariadi, Mas'ud Hermin Ratnani Herry Agoes Hermadi Hidajati, Nove IMAM MUSTOFA Indira Vania Carisya Ismudiono Ismudiono, Ismudiono Isti Annisa Ramahtia Iwan Sahrial Hamid Kabayo, Fierda Kusriningrum Rochiman Masruroh, Laili Salisa Mirni Lamid Mochamad Lazuardi Mohamad Safri Sauqi Mujahid Mujahid, Mujahid Mustofa Helmi Effendi Nove Hidajati Nunuk Dyah Retno Lastuti Nusdianto Triakoso Pahlevy, Jolly Rifqi Poedji Hastutiek Pramesti, Nugraheni Anggara Prastiya, Ragil Angga Prastyorini, Vina Ari Pudji Srianto Rahmawati, Mitha Ardila Rimayanti Rimayanti Rochmah Kurnijasanti Samuel Inioluwa Akeju Saputro, Amung Logam Setiawan Koesdarto Setyadi, Arda Wahyu Setyawan, Firman Shinta Putri Quraini Soeharsono Soeharsono Sri Mumpuni Sosiawati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Supranianondo, Koesnoto Suzanita Utama Tatik Hernawati Tehupuring, Benjamin Chr Tias, Eriza Putri Ayu Ning Tita Damayanti Lestari Tri Wahyu Suprayogi Trilas Sardjito Umama, Ayu Riza Ummaisyah, Winadya Reika Vladika, Maulinda Agchirilia Wahjuni, Retno Sri Warsito, Sunaryo Hadi Wibawati, Prima Ayu Wicaksono, M Galih Widiyatno, Thomas V. Widjiati Widjiati, Widjiati Widodo, Oky Setyo Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wurlina, W Yohana, Nadia Yudhana, Aditya Yulianti Mauludy Utami

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