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All Journal Biotika: Jurnal Ilmiah Biologi Indonesian Journal of Applied Sciences Zuriat Jurnal Ilmu Dasar AL KAUNIYAH JURNAL ISTEK Jurnal Florea Jurnal Teknik Sipil Pro-Life Edumatsains Jurnal Bioedukasi Kultivasi Jurnal Biodjati Jurnal Biologi Tropis JMM (Jurnal Masyarakat Mandiri) Jurnal Kreativitas PKM Manuju : Malahayati Nursing Journal Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Geodika: Jurnal Kajian Ilmu dan Pendidikan Geografi JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI KAIBON ABHINAYA : JURNAL PENGABDIAN MASYARAKAT Didaktik : Jurnal Ilmiah PGSD STKIP Subang Sains dan Matematika Indonesian Journal of Global Health research Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis Indonesian Journal of Adult and Community Education (IJACE) Journal of Comprehensive Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Dewantara: Jurnal Pendidikan Sosial Humaniora Jurnal Asuhan Ibu dan Anak Jurnal Keperawatan 'Aisyiyah Indonesian Research Journal on Education Manilkara: Journal of Bioscience Calakan: Jurnal Sastra, Bahasa, dan Budaya Jurnal Budiman: Pembangunan dan Pengabdian Masyarakat Nusantara Jurnal Riset dan Inovasi Pendidikan Sains (JRIPS) Jurnal Inovasi Bahan Lokal dan Pemberdayaan Masyarakat Jurnal Sains dan Kesehatan
Tia Setiawati
Departemen Biologi Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Padjadjaran
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Journal : AL KAUNIYAH

 1 
PENGARUH POLYETHYLENE GLYCOL (PEG) TERHADAP KADAR KUERSETIN KULTUR KALUS Chrysanthemum morifolium Ramat PADA KONDISI PENCAHAYAAN BERBEDA Tia Setiawati; Syifa Fauzia Zazuli; Annisa Annisa; Mohamad Nurzaman; Budi Irawan
Al-Kauniyah: Jurnal Biologi Vol 13, No 1 (2020): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3713.676 KB) | DOI: 10.15408/kauniyah.v13i1.13688

Abstract

AbstrakKrisan (Chrysanthemum morifolium Ramat) mengandung senyawa kuersetin dengan efek farmakologi yang sangat luas. Penambahan polyethilene glycol (PEG) dapat dimanfaatkan untuk meningkatkan produksi metabolit sekunder secara in vitro. Penelitian ini bertujuan untuk mengetahui pengaruh PEG terhadap pertumbuhan kalus krisan dan kadar kuersetin 3-O-rhamnosida pada pencahayaan yang berbeda. Penelitian menggunakan Rancangan Acak Lengkap (RAL). Eksplan kalus berumur 45 hari setelah tanam (HST) disubkultur pada media MS + 4 ppm 2,4-D dengan penambahan PEG dalam lima taraf konsentrasi yaitu 0, 10, 20, 30, dan 40 ppm. Kultur diinkubasi pada kondisi gelap dan terang. Parameter yang diamati adalah warna, tekstur, ukuran, berat basah, dan berat kering kalus serta kadar kuersetin 3-O-rhamnosida. Data kuantitatif dianalisis menggunakan Analisis Varians dan Uji Jarak Berganda Duncan (α 5%), sedangkan data kualitatif dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa kalus pada kondisi terang berwarna cokelat dan cokelat kehijauan, sedangkan pada kondisi gelap berwarna putih kecokelatan. Perlakuan 10 ppm PEG menghasilkan berat basah dan berat kering kalus tertinggi baik pada kondisi terang maupun gelap berturut-turut 1,97 g dan 2,92 g; 0,94 g dan 1,09 g. Kadar kuersetin 3-O-rhamnosida tertinggi pada kondisi gelap dan terang terdapat pada perlakuan 10 ppm PEG berturut-turut  1,72 µg/g berat kering (BK) dan 2,59 µg/g BK.Abstract Chrysanthemum morifolium Ramat. contains quercetin with very broad pharmacological effects. The addition of PEG can be used to increase the production of secondary metabolites using in vitro method. This study aimed to determine the effect of PEG on the growth of Chrysanthemum callus and quercetin 3-O-rhamnoside content in different lighting conditions. The experimental design used was a Completely Randomized Design. After 45 days, callus were subcultured on MS medium + 4 ppm 2,4-D which PEG was added in five concentration levels (0, 10, 20, 30, and 40 ppm). Culture was incubated in dark and light conditions. Parameters observed were color, texture, size, wet weight and dry weight of callus, also quercetin 3-O-rhamnoside levels. Quantitative data was analyzed using Analysis of Variance and Duncan's Multiple Distance (α 5%). Qualitative data was analyzed descriptively. The results showed that in light condition, the callus has brown and greenish brown color, whereas in dark, it has brownish white color. The 10 ppm PEG treatment produced the highest fresh weight and dry weight in both light and dark conditions of 1.97 g and 2.92 g, 0.94 g and 1.09 g, respectively.The highest quercetin 3-O-rhamnoside content  in dark and light conditions were founded in 10 ppm PEG treatment of 1.72 µg/g dry weight (DW) and 2.59 µg/g DW.
Co-Authors , ,. Mulyadi , ,. Suryana Adityawan, Mohammad Bagus Adnin Laila Agung Karuniawan Agus Irpan Nurhidayat Agustine, Leony Alfira, Ajeng Alma Ayalla Amala Lastari Utami Anandira Witri Andiana, Anisa Andri Irawan Anggita Levi Astuti Anggita Leviastuti Ani Lestari Ani Lestari Anisaa Fauziyah Annisa Annisa Annisa Annisa Annisa Annisa Annisa Nur Arofah Arianti, Tanti Arofah, Annisa Nur Asep Zainal Mutaqin Astuti, Sipa Dwi Auliya Zahra Awali, Dian Siti Ayalla, Alma Azifah An’amillah Bari, Ichsan Budi Irawan Budi Irawan Darmawan, Darwis Debby Ustari Dede Winingsih Delisa Lestari Desak Made Malini Devitri Amisa Dian Latifa Ela Noviani Elah Karimah Eli Lusiani Endang Rosdiana Fadhilla Ramadhona Farhurohman, Oman Fatmawati, Ariani Fikriyah, Nanda adzka Fitria, Sania Septiani Fredella Josenia, Carlen Harkunti P. Rahayu, Harkunti P. Hendi N Wicaksono Heri Kurniawan, Heri Hernandi Sujono Huda, Ida Nurul Ibadurrahman, Rifki Inggriane Puspita Dewi, Inggriane Puspita Inneke Febrihardianti Syamsi Irma Wahyuningsih Irvan Herdiana Jannah, Isma Aenul Johan Iskandar Joko Kusmoro Kartiawati Alipin Kartiawati Alipn Kharismala R, Nurlaila Lia Dewi Juliawaty Libriati, Rika Lusiani, Eli Maitala Sari Marcelline, Karen Marheny Lukitasari Marheny Lukitasari Maryam Alifah Maulani, Susan Maulidiyah Maulidiyah Maya Amalia Meilinda, Pudji Melina Melina Moahamad Nurzaman Moahamad Nurzaman, Moahamad Moch. Nurzaman Mohamad Nurzaman Mohammad Farid Muhammad Syahril Badri Kusuma, Muhammad Syahril Badri Nia Rossiana Nining Ratningsih Nisa Hurin Nisa Hurin Nopriyeni Nopriyeni Nopriyeni, Nopriyeni Noviani, Ela Noviyanti Soleha Nuriman, Muhammad Nurullia Fitriani Nurullia Fitriani Otih Rostiana Putri, Deviana Aulia Rachma, Nadhira Zaachrany Radewi Safira Fauzia Radewi Safira Fauzia, Radewi Safira Rahmawati, Fitryasari Rani Rubiyanti, Rani Rasmiaditya Silasari Revita Yanuarsari Rezekikasari, Rezekikasari Rike Yuniaris Rully Budiono Ruly Budiono Rusdi Hasan Rusdi Hasan Rusdi Hasan Saeful Amin, Saeful Senadi Budiman Sephianti, Selvi Siti Mariyani Sofiyah, Yusi Sri Mulyati Sri Ratu Dewi Pridani Sugarti, Dini Sukrido Sukrido Supriatun, T Syifa Fauzia Zazuli T Supriatun Tentani Buhti Amadea Titin Supriatun Titin Supriatun Titin Supriatun Titin Supriatun Sadeli Valentina Adimurti Kusumaningtyas Vera Octavia Subardja Washfanisa, Handina Alya Wibowo, Sigit Sapto Wicaksono, Hendi N Winingsih, Dede Yana Maolana Syah Yana Maolana Syah Yani Astuti Yuliana, Trisna Yuniar, Muthya Yuniaris, Rike Yusi Sofiyah