Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

5.315

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Buletin Hama dan Penyakit Tumbuhan Agrikultura Jurnal Natur Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Hortikultura Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat AGRIVITA, Journal of Agricultural Science Journal of ICT Research and Applications Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Agrotek Tropika JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Entomologi Indonesia Agro Bali: Agricultural Journal Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Proceedings Series on Physical & Formal Sciences

SRI HENDRASTUTI HIDAYAT

Departemen Proteksi Tanaman, Fakultas Pertanian, IPB Jl. Kamper Kampus IPB Dramaga, Bogor 16680

Author-ID : 385601

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Veterinary

Published : 106 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4 5

Response of Various Tomato Genotypes to Begomovirus Infection and Its Improved Diagnostic NOOR AIDAWATI; SRI HENDRASTUTI HIDAYAT; PURNAMA HIDAYAT; RUSMILAH SUSENO; SRIANI SUJIPRIHATI
HAYATI Journal of Biosciences Vol. 14 No. 3 (2007): September 2007
Publisher : Bogor Agricultural University, Indonesia

Begomovirus infection was identified from tomato growing areas in West Java (Bogor), Central Java (Boyolali), and D.I. Yogyakarta (Kaliurang). Efforts to reduce the infection among others are planting resistance varieties. This research was undertaken to evaluate 14 tomato genotypes for their response to the infection. Dot blot hybridization using nonradioactive (digoxigenin) DNA probe was employed to determine the presence of begomovirus in inoculated plants. Polymerase chain reaction-amplified product of DNA clone of tobacco leaf curl virus Ã¢â‚¬â€œIndonesia was used as a source of DNA probe. All of tomato genotypes evaluated in this study was infected separately by three strain of begomovirus (GVPSlm, GVABy, GVCBgr). Tomato genotypes Bonanza, Jelita, Safira, Permata, Presto, PSPT 8, PSPT 5B, Apel-Belgia, Karibia, Mitra, PSPT 9, Marta, and PSPT 2, showed susceptible or highly susceptible response to the three strains of begomovirus. Exception to those was shown by cv. Intan which resulted in moderate resistance when inoculated with GVCBgr although it resulted susceptible response with the other two strains. Dot-blot hybridization technique was proved to be a powerful tool to detect begomovirus infection in plants showing symptom as well as symptom-less plants. Accumulation of the virus in those plants was relatively high, except in cv. Bonanza and Apel-Belgia. Dot-blot hybridization technique using DIG-labeled DNA probe was able to detect begomovirus DNA in infected tissue up to 10-2 dilution factor. Key words: Geminivirus, hybridization, resistance, tomato

Molecular Identification and Sequence Analysis of Tobacco Leaf Curl Begomovirus from Jember, East Java, Indonesia SRI HENDRASTUTI HIDAYAT; ORAWAN CHATCHAWANKAN PANICH; NOOR AIDAWATI
HAYATI Journal of Biosciences Vol. 15 No. 1 (2008): March 2008
Publisher : Bogor Agricultural University, Indonesia

Begomovirus had been proved as the causal agent of leaf curl disease in tobacco in Indonesia, or commonly in Indonesia called as penyakit krupuk tembakau. Association of Begomovirus with the disease was further confirmed by sequence analysis. Amplification of the virus was conducted following whitefly (Bemisia tabaci Genn.) transmission. Fragment of DNA 1.6 kb was amplified by polymerase chain reaction (PCR) located within the replication initiator protein gene and coat protein gene (top region). Conserved sequence of stem loop region was found, included nonanucleotide sequence TAATATTAC present in all geminiviruses. Begomovirus associated with leaf curl disease in tobacco showed the closest relationship with Ageratum yellow vein virus - Zimbabwe, a strain of Tobacco leaf curl virus from Southern Africa. It was also known that Begomovirus associated with leaf curl disease in tobacco from Jember, East Java was different from other Indonesian Begomoviruses reported earlier. Key words: Begomovirus, leaf curl disease

Expression of Recombinant Sugarcane Streak Mosaic Virus Coat Protein Gene in Escherichia coli Hamdayanty Hamdayanty; Sri Hendrastuti Hidayat; Tri Asmira Damayanti
HAYATI Journal of Biosciences Vol. 23 No. 3 (2016): July 2016
Publisher : Bogor Agricultural University, Indonesia

Sugarcane streak mosaic virus (SCSMV) is an important virus causing mosaic disease in sugarcane and transmitted through the cutting cane. Commercial antiserum to detect SCSMV and to monitor the disease development is not available. The research was conducted to produce antigen of SCSMV coat protein (SCSMV-CP) through overexpressing it on bacterial expression which will be used for antiserum production. SCSMV-CP was amplified using specific primers for CP gene containing BamHI and HindIII restriction enzyme sites and cloned into pTZ57R/T. Subsequently, the SCSMV-CP was subcloned into pET28a and transformed on Escherichia coli BL21(DE3) and Rosetta-gami(DE3)pLysS. The concentration of isopropyl β-d-thiogalactopyranoside (IPTG), incubation temperature, and bacterial harvesting time after IPTG induction were optimized. SCSMV-CP gene was successfully amplified with size ±855 bp, subcloned into vector expression, and expressed in insoluble fraction either in both bacterial host. Optimal protein expression of SCSMV-CP recombinant was obtained at 25°C with IPTG concentration 0.25–1.00 mM and harvested at 9–12 hours after IPTG induction in E. coliBL21(DE3), and at 30°C with IPTG concentration 0.25–1.00 mM and harvested 3–12 hours after IPTG induction in E. coli Rosetta(DE3)pLysS. SDS-PAGE analysis showed that protein size of SCSMV-CP recombinant was ±35.4 kDa.

Penyebaran Virus Utama Tebu di Lampung dan Sulawesi Selatan Dwi Subekti; Sri Hendrastuti Hidayat; Tri Asmira Damayanti; Purwono Purwono
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 1 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

One effort to increase domestic sugar production is through the extension of sugarcane growing area outside Java island. The status of sugarcane disease outside Java, particularly those caused by virus(es), has not been evaluated yet; where virus diseases are able to decrease sugarcane productivity. Therefore, the aim of this research was to identify the sugarcane major viruses and their distributions in Lampung and South Sulawesi. Field survey and leaf sampling was collected from Lampung Tengah District, in Lampung Province and three districts in South Sulawesi Province i.e., Bone, Gowa, and Takalar. Virus detection was performed by reverse transcription-polymerase chain reaction (RT-PCR) method using specific primers for Sugarcane mosaic virus (SCMV), Sugarcane streak mosaic virus (SCSMV), and Sugarcane yellow leaf virus (SCYLV). Results indicated that symptoms variation was observed in the field. The highest disease incidence and severity was found in Lampung, i.e. 100% and 61.67-71.67%, respectively; whereas the lowest disease incidence and severity was found in Takalar, i.e. 5-70% and 5-45%, respectively. The disease incidence and severity of viral diseases tend to be low in dry and low rain fall conditions. This information might be helpful for the extensification program of sugarcane development in supporting the sugar self-sufficiency program. Keywords: disease incidence, disease severity, reverse transcription-polymerase chain reaction, sugar self-sufficiency

Pengaruh Empat Galur Bakteri Pemacu Pertumbuhan Tanaman dan Waktu Inokulasi Virus terhadap Keparahan Penyakit Daun Keriting Kuning Cabai Hari Priwiratama; Sri Hendrastuti Hidayat; Widodo .
Jurnal Fitopatologi Indonesia Vol. 8 No. 1 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Plant growth-promoting rhizobacteria (PGPR) has effectively reduced infection of pathogens. Bacillus polimixa BG25, B. subtilis SB3, Pseudomonas fluorescens PG01, P. fluorescens S32, and their combinations were used as seed treatment. Bemisia tabaci (Hemiptera: Aleyrodidae) was used to inoculate Geminivirus at 3, 5, and 6 weeks after planting. Seeds without any bacteria treatment were used as controlled plant. Treatment of PGPR and time of inoculation were significantly effect disease intensity, but not incubation period. Late infection of Geminivirus reduced disease intensity. Based on measurement of plant height, number of branch, and flowers it was concluded that some bacterial treatments was significantly effect plant growth. Key words: Bemisia tabaci, Geminivirus, PGPR, plant growth promoting rhizobacteria

Evaluasi Tiga Metode Preparasi RNA Total untuk Deteksi Turnip mosaic potyvirus dari Benih Brassica rappa dengan Reverse Transcription-Polymerase Chain Reaction Jati Adiputra; Sri Hendrastuti Hidayat; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol. 8 No. 2 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Turnip mosaic virus (TuMV) is an important seedborne virus that infects vegetable crops, especially Brassicae group, in Indonesia. Reliable detection method having high accuracy is required to detect the virus from seeds in order to avoid disease incidence as early as possible. A study was conducted to evaluate three total RNA extraction methods from Brassica seeds for TuMV detection using reverse transcription- polymerase chain reaction. The samples used are Brassica rappa seeds and seedling germinated for 3, 5, and 7 days. Total RNA extraction methods evaluated consisted of Wylie method, Randles method, and commercial kit as comparation. Total RNA extracted using Wylie and Randles methods has high concentration but low level of purity, on the other hand total RNA obtained using commercial kit has low concentration but high level of purity. Detection of TuMV from seed was successfully carried out using Wylie and Randles methods, TuMV from 3 - day old seedlings was detected using all three methodes, TuMV from 5-day old seedlings was only detected using Randles method, whereas TuMV from 7-day old seedlings was not detected using all three methodes. Total RNA extraction method using Wylie and Randles methodes is recommended for detection of TuMV from caisin seed using RT-PCR technique. Key words: Brassica rappa seed, reverse transcription- polymerase chain reaction, Total RNA, Turnip mosaic potyvirus

Penularan Potyvirus Penyebab Penyakit Mosaik pada Tanaman Nilam melalui Vektor Aphis gossypii Rita Noveriza; Gede Suastika; Sri Hendrastuti Hidayat; Utomo Kartosuwondo
Jurnal Fitopatologi Indonesia Vol. 8 No. 3 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Patchouli (Pogostemon cablin) is an economically important plant due to its capability to produce essential oil, i.e. patchouli oil. Pest and diseases may contribute to low production of patchouli oil in Indonesia. Aphids are commonly found associated with patchouli plants showing mosaic symptoms, indicated possible relationship between aphids and mosaic virus. Early diagnosis showed that mosaic disease on patchouli is caused by Potyvirus infection. A study was conducted to identify those aphid species and to examine relationship between the aphid species and mosaic virus. Two aphid species commonly found in patchouli plants in Indonesia are Aphis gossypii and Brachycaudus sp. Further study on the ability of A. gossypii to transmit Potyvirus revealed the possible non-persistent relationship, with 25 min optimum pre-acquisition starvation, 15 min acquisition period and 4 hours optimum inoculation feeding period, respectively.Key words: Aphis gossypii, Brachycaudus, nilam, Pogostemon cablin, Potyvirus

Ketahanan Biokimia Tanaman Cabai terhadap Begomovirus Penyebab Penyakit Daun Keriting Kuning Rokhana Faizah; Sriani Sujiprihati; Muhamad Syukur; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 8 No 5 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Plant biochemical resistance towards virus infection can be evaluated through measurement of salicylic acid accumulation and peroxidase concentration. A research was conducted to study biochemical resistance of chillipepper to infection of Pepper yellow leaf curl Begomovirus (PYLCV) the causal agent of pepper yellow leaf curl disease. Virus inoculation was done using insect vector, Bemisia tabaci. Salicylic acid accumulation and peroxidase concentration on 6 chillipepper genotypes (35C2, IPB C15, IPB C26, IPB C10, IPB C14, dan IPB C12) was measured 120 h after inoculation. The results showed that salicylic acid accumulation and peroxidase concentration were higher on plants after virus infection. Susceptible genotypes (35C2, IPB C15, IPB C26) tend to have higher salicylic acid accumulation and peroxidase concentration than those of resistant genotype (IPB C12). Positive correlation was observed between biochemical resistance (salicylic acid accumulation) and structural resistance (length of palisade meshophyll cell).Key words: Begomovirus, Pepper yellow leaf curl virus, peroxide enzyme, resistance mechanism, salicylic acid

Infeksi Papaya ringspot virus pada Tanaman Pepaya di Provinsi Nanggroe Aceh Darussalam Sri Hendrastuti Hidayat; Sari Nurulita; Suryo Wiyono
Jurnal Fitopatologi Indonesia Vol 8 No 6 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Severe mosaic symptoms was observed on papaya plants in Meusa village, Kutablang sub district, Bireun district and Lambaro Teunom village, Lembah Seulawah sub district, Aceh Besar district. Systemic mosaic was found in leaves, stem, twig, and fruit. Reverse transcription-polymerase chain reaction (RT-PCR) was conducted to detect the virus causing mosaic symptoms using universal primer for Potyvirus. Amplification of 320 bp DNA fragment was successfully obtained from leaves and fruits bearing severe mosaic symptoms. Nucleotide sequencing was proceeded using the DNA amplicon as the template. Further sequence analysis indicated that Potyvirus isolates from papaya in Aceh show the highest homology (92.7% to 94.7%) with Papaya ringspot virus (PRSV) from Philippines, Thailand, and Indonesia. This is the first report on PRSV infection on papaya in Nanggroe Aceh Darussalam province. Key words: Potyvirus, RT-PCR, sequencing

Tomato yellow leaf curl Kanchanaburi virus Penyebab Penyakit Mosaik Kuning pada Tanaman Terung di Jawa Tega Kintasari; Dwi Wiyati Nurul Septariani; Sri Sulandari; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Yellow and yellow mosaic symptoms was observed on eggplant (Solanum melongena) around Bogor, Bandung, Pati, Rembang, and Bantul during a survey in early 2013. Polymerase chain reaction based detection was carried out using total DNA isolated from symptomatic leaf samples and a pair of Begomovirus-universal primers, SPG1/SPG2. The expected size (~900 bp) amplicon was detected from all five symptomatic samples, indicating the presence of Begomovirus infection. Each amplicon was sequenced followed by basic local alignment search tool analysis. It was revealed that the sequence had the highest homology (98.8%) with Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV) sequence. This is the first report on TYLCKaV infection on eggplant in Indonesia.

Co-Authors . SUDARSONO Abdul Muin Adnan ABDUL MUNIF ALI NURMANSYAH Amelia Feryna Bulan Dini Ana Septiana Saputri Anas Dinurrohman Susila Aqlima , Aqlima, nFN Arifin Tasrif Asmar Hasan Asniwita Asniwita Astri Windia Wulandari Wulandari ATI SRI DURIAT Awang Maharijaya Ayu Kartini Parawansa Bambang S. Purwoko Bambang Sapta Purwoko Bonny Poernomo Wahyu Soekarno Bonny Poernomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Budi Tjahjono Darni Rambu D. Siala Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Diny Dinarti Dono Wahyuno Dono Wahyuno Dwi Astuti DWI SUBEKTI Dwi Subekti Dwi Wiyati Nurul Septariani Dwiwiyati Nurul Septariani Efendi, Darda Efi Toding Tondok Eliza Suryati Rusli Endang Nurhayati Endang Nurhayati Evan P. Ramdan Farida, Naimatul Fitrianingrum Kurniawati, Fitrianingrum Gede Suastika Gede Suastika GEDE SUASTIKA GEDE SUASTIKA Gede Suastika Gede Suastika Giyanto Giyanto Giyanto Hamdayanty Hamdayanty Hanif, Andini Hari Priwiratama Harwan Susetio Heriyanto Syafutra I Wayan Winasa Ifa Manzila Ifa Manzila Ika Mariska Ika Mariska Irsan Nuhantoro Ishak Manti ISHAK MANTI Isti Wulandari Jamsari Jamsari Jati Adiputra John Thomas, John Joni Hidayat, Joni Jumanto Harjosudarmo Jumanto Harjosudarmo JUMANTO HARJOSUDARMO Jumsu Trisno Kadwati Kadwati Kikin H Mutaqin KIKIN HAMZAH MUTAQIN Ladja, Fausiah T. Laksono Trisnantoro Listihani, Listihani Mawarni, Sofi Meity S Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Melinda . Meliyana Memen Surahman Miftakhurohmah Miftakhurohmah Miftakhurohmah Mimi Sutrawati Muh. Taufik Muhamad Syukur Muhammad Herman Muhammad Taufik MUHAMMAD TAUFIK Muhammad Taufik Nada, Azmi Khoirin Neni Gunaeni Niken Nur Kasim, Niken Nur Nissa Fawwaz Adilah NOOR AIDAWATI ORAWAN CHATCHAWANKAN PANICH Prabawati Hyunita Putri Puji Lestari PURNAMA HIDAYAT Purwoko, Bambang Sapto Purwono Purwono Rahayuwati, Sat RAHMI YUNIANTI Ramdan, Evan Purnama RAUF, AUNU Redy Gaswanto Redy Gaswanto, Redy Refa Yulianingsih Reymas M.R. Ruimassa Rina Rachmawati Rita Noveriza Rita Noveriza Rita Noveriza RITA NOVERIZA Rizqiyah, Sakinah Inayatur Rokhana Faizah Roy Ibrahim RR. Ella Evrita Hestiandari Rusmilah Suseno RUSMILAH SUSENO Rustiani, Ummu S. Saiful Akhyar Lubis Sari Nurulita Sari Nurulita Sari, Rahmah Dian Sarsidi S astrosumarjo Sarsidi Sastrosumarjo Satya Nugroho Sayekti, Tri Wahono Dyah Ayu Sherli Anggraini Sientje Mandang Sumaraw Siregar, Ivan Arif Rachman Martua Siti Hafsah Siti Shofiya Nasution Slamet Susanto Sobir Sobir Soemartono Sosromarsono Soemartono Sosromarsono Sri Hartati Sri Hartati Sri Hartati Sri Sulandari Sri Sulandari Sri Sulandari SRIANI SUJIPRIHATI Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Sugeng Santoso Supramana Suryo Wiyono Susanti Mugi Lestari Tega Kintasari Titiek YULIANTI TRI ASMIRA DAMAYANTI TRI JOKO SANTOSO Trikoesoemaningtyas Trimuri Habazar TRIMURTI HABAZAR Triyani Dumaria Tutik Harmiyati, Tutik Ummu S. Rustiani Ummu Salamah Rustiani Utomo Kartosuwondo UTOMO KARTOSUWONDO Utomo Kartosuwondo UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO Vinsen Willi Wardhana Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Willing Bagariang Zahratul Millah

Title

Found 106 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 106 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 106 Documents
Search