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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Buletin Hama dan Penyakit Tumbuhan Agrikultura Jurnal Natur Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Hortikultura Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat AGRIVITA, Journal of Agricultural Science Journal of ICT Research and Applications Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Agrotek Tropika JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Entomologi Indonesia Agro Bali: Agricultural Journal Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Proceedings Series on Physical & Formal Sciences

SRI HENDRASTUTI HIDAYAT

Departemen Proteksi Tanaman, Fakultas Pertanian, IPB Jl. Kamper Kampus IPB Dramaga, Bogor 16680

Author-ID : 385601

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Veterinary

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Cendawan Endofit Nonpatogen Asal Tanaman Cabai dan Potensinya sebagai Agens Pemacu Pertumbuhan Evan P. Ramdan; Widodo Widodo; Efi Toding Tondok; Suryo Wiyono; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 9 No 5 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Endophytic fungi have been reported to have mutual symbiosis with their host. This research aimed to obtain nonpathogenic isolates of endophytic fungi from roots, stems, and branches of chili pepper. Endophytic fungi were isolated on 10% malt extract agar (MEA) after serial surface sterilization of plant parts. The effect of endophytic fungi on the development of chili pepper seeds were tested by growing seeds on media containing endophytic fungi isolate. As many as 138 isolates of endophytic fungi were successfully isolated from chili pepper plants from Garut and Bogor. Based on colony morphology the isolates can be differentiated into 9 morphotypes. Among the total isolates, 13.04% are nonpathogenic. Some of them (7,25%) do not effect seedling significantly compared to control and some isolates (5.79%) caused better seedling growth than control. Endophytic fungi having the potential to promote plant growth was identified as Aspergillus sp., Fusarium sp., Penicillium sp., sterile hyphae 1, 2, and 3.

Penggunaan Galur Lemah Chili veinal mottle virus untuk Proteksi Silang Asniwita Asniwita; Sri Hendrastuti Hidayat; Gede Suastika; Slamet Susanto; Sriani Sujiprihati
Jurnal Fitopatologi Indonesia Vol 9 No 5 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Inoculation of mild virus strain prior to severe virus strain to protect plant against viral disease is the principle of cross protection. Five mild strains of Chili veinal mottle virus (ChiVMV), i.e. -KAR, -SPR, -SKT, -CSR, and -PGL were used as cross protection agent to protect chili pepper plants against severe strain infection of ChiVMV-CKB. The mild strains were inoculated mechanically prior inoculation of severe strain and the efficiency of cross protection was evaluated by observing symptom development and measuring crop yield. Inoculation of mild strains 7 days prior inoculation of severe stain was not able to protect the plant from infection of severe strain ChiVMV-CKB. Protective effect was observed when mild strains were inoculated at 14, 21, and 28 days prior inoculation of severe strain. Symptom development was suppressed or delayed, and crop yield was not significantly different with healthy plants. It was suggested that to obtain the best protection against severe strain, the mild strain should be applied as early as possible before the occurrence of severe strain infection.

Sensitivitas Metode Serologi dan Polymerase Chain Reaction untuk Mendeteksi Bean Common Mosaic Potyvirus pada Kacang Panjang Sherli Anggraini; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 10 No 1 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Mosaic disease in yard long bean is caused by Bean common mosaic potyvirus (BCMV) and has been reported to affect yield. Common method to detect infection of BCMV involves serological assay and polymerase chain reaction (PCR). The aims of this research is to assess the sensitivity of three methods, i.e. Indirect Enzym-Linked Immunosorbent Assay (I-ELISA), Dot Immunobinding Assay (DIBA), and reverse transcription (RT)-PCR as detection method for BCMV infection in yard long bean. Sensitivity level of the methods was evaluated by diluting plant extract and antisera for I-ELISA and DIBA, and cDNA as template in RT-PCR. Virus isolate from Cirebon was maintained in yard long bean in screenhouse and used for the assessment. Absorbance value of ELISA showed that dilution end point for I-ELISA was reached at 10-3 and 10-2 of plant extract and antisera dilution, respectively. Positive infection was still detected using DIBA when the plant extract was diluted up to 10-5 based on development of color intensity on nitrocellulose membrane. Specific viral DNA fragment was still amplified when cDNA was diluted up to 10-4, indicated higher sensitivity level of RT-PCR method.

Respons Lima Varietas Kacang Panjang terhadap Bean common mosaic virus Harwan Susetio; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 10 No 4 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Incidence of yellow mosaic disease on yard long bean occurred widely in some areas in West and Central Java in 2008–2009. The causal agent of this disease is Bean common mosaic virus (BCMV) which can be transmitted through seed and aphids. Research was conducted to determine response of five varieties of yard long bean (Vigna sinensis), i.e. Parade, New Jaliteng, Long Silk, Super Sainan, and Pilar to BCMV infection. Yard long bean plants was inoculated by BCMV mechanically, followed by observation of disease incidence and severity, incubation period, flowering time, and yield. All five varieties of yard long bean showed susceptible response to BCMV infection. Incubation period was 6–16 days, disease incidence was 90–100%, and disease severity was 49.1–69.7%. BCMV infection caused severe symptoms of leaf malformation and plant stunting. Flowering time was delayed 2–5 days on infected plants and yield was decreased up to 46.6%. Breeding program to develop resistance varieties to BCMV is necessary.

Keparahan Penyakit Daun Keriting Kuning dan Pertumbuhan Populasi Kutukebul pada Beberapa Genotipe Cabai Nissa Fawwaz Adilah; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 10 No 6 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Incidence of yellow leaf curl disease on chili pepper is very common in Indonesia and becomes major production constrain. The causal agent, Pepper yellow leaf curl virus (PYLCV), is only transmitted by insect vector, i.e. whitefly (Bemisia tabaci). Research was conducted to determine response of 6 chili pepper genotypes [Meteor, Rimbun, Tornado, F1(12X14), IPBC12, and 35C2] against infection of PYLCV and population growth of whitefly on each genotypes. Transmission of PYLCV to chili pepper genotypes was done using whitefly. Whitefly was maintained on each chili pepper genotypes to evaluate its population growth. Genotype IPBC12 was resistant to PYLCV having disease severity 0–9.2% and showed mild symptom. Genotypes Rimbun, Meteor, Tornado, F1(12X14), and 35C2 were susceptible having disease severity >20% and showed severe symptom. Resistance of IPBC12 might be correlated with the growth of whitefly on the genotype. The amount of whitefly eggs and its successful rate to become adult was relatively lower in IPBC12 than those in other genotypes. Genotype IPBC12 might be considered as resistance source in developing resistant variety of chili pepper to PYLCV.

Deteksi Virus Utama Bawang Merah dan Bawang Putih dari Daerah Jawa Barat dan Jawa Tengah Kadwati Kadwati; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 11 No 4 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Viral disease has been reported to cause significant effect on production of shallot (A. cepa) and garlic (A. sativum). The study was conducted to detect main viruses from leaves and bulbs of shallot and garlic using specific antibodies by ELISA method. Leaf and bulb samples was collected from West Java (Bandung, Bogor and Cirebon), Central Java (Brebes), and Yogyakarta (Bantul). Single as well as mix infection of GCLV, SLV, and Potyvirus was successfully detected using specific antibodies. The average percentage of virus infection in the crop ranged from 11.2–14.3% on shallot, and 14.3% on garlic; whereas in the bulb ranged from 11.2–13.3% on shallot, and 9.18% on garlic.

Identifikasi Virus Penyebab Penyakit Kerdil pada Tanaman Padi di Sukamandi, Jawa Barat Amelia Feryna Bulan Dini; I Wayan Winasa; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 11 No 6 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Viral diseases on rice is an important constraint for rice production in Indonesia. Research was conducted to determine the incidence of stunting disease in Sukamandi area at Subang regency (West Java), to identify the virus associated with the disease using RT-PCR method, and to analyze coat protein gene sequences. Field observation during growing period on November– December 2014 indicated that the incidence of stunting disease was low (0.01-10.52%). Grassy stunt and ragged stunt symptoms was observed in the fields. Spesific DNA fragments of coat protein gene of Rice ragged stunt virus (RRSV) and Rice grassy stunt virus (RGSV) was successfully amplified using specific primers. Nucleotide sequence analysis showed that RRSV and RGSV isolates from Subang has the highest homology with RRSV isolates from Vietnam, Philipines and Thailand (97.1 %) and RGSV isolate from Longan, Vietnam (95.8%), respectively.

Penggunaan Pelacak DNA untuk Deteksi Papaya ringspot virus dengan Metode Hibridisasi Asam Nukleat Irsan Nuhantoro; Sri Hendrastuti Hidayat; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol. 14 No. 3 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Use of DNA Probe for Detection of Papaya ringspot virus Using Nucleic Acid Hybridization MethodPapaya ringspot caused by Papaya ringspot virus (PRSV) is one of the most destructive diseases of papaya. The disease had not been found in Indonesia, until disease outbreak in Nangroe Aceh Darussalam was reported in 2012. Since then, the disease spread rapidly in most papaya growing areas in Sumatera, Java and Bali. Papaya ringspot virus (PRSV) is generally detected using serological or polymerase chain reaction methods. Improvement in detection method is necessary to facilitate a more reliable tool for controlling the spread of PRSV. The aim of the research was to construct DNA probe for development of detection method based on nucleic acid hybridization. Molecular characterization based on HCPro gene sequence indicated high homology (97.88 to 99.05%) among PRSV isolates from Boyolali (Central Java), Medan (North Sumatera), Sleman (Yogyakarta) and Tabanan (Bali). Two DNA clones of HCPro gene were selected for probe construction and the probes were then labeled using PCR DIG-dioxigenin. Optimization of nucleic acid dot blot hybridization method to achieve strongest positive reaction was developed, i.e. using stringency washes at 1×SSC, 0.1% SDS, incubation at 60 oC for 15’. The DNA probe for PRSV has a high specificity and sensitifity; it could detect PRSV at the lowest concentration of nucleic acid (0.062 µg µL-1).

Potensi Cendawan Endofit sebagai Pengendali Hayati Penyakit Busuk Pangkal Batang (Phytophthora capsici) pada Bibit Cabai Evan Purnama Ramdan; Efi Toding Tondok; Suryo Wiyono; Sri Hendrastuti Hidayat; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol. 13 No. 5 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Stem rot disease caused by Phytophthora capsici is an important disease on chilli. Eight endophytic fungi that had been isolated and screened based on pathogenecity test were further tested for their potential as the biological control agent of the stem rot disease of chilli. The endophytic fungi suspension was applied twice during the trial. The first application was on 100 seed lot, by soaking them in 100 mL of suspension. The second application was on the 3 weeks-old chili seedlings by drenching them with 10 mL suspension per plant. The concentration of endophytic fungi in the suspension was 2.8 × 106 cfu mL-1. The disease intensity and AUDPC value were measured for 4 weeks after the pathogen inoculation. The growth inhibition test of P. capsici was performed in vitro and the colonization abilities of endophytic fungi were observed at 4 weeks-old chilli seedlings. Eight endophytic fungi inhibited the growth of the P. capsici, and two of those isolates namely Penicillium strain MAG1 and Penicillium strain PAB2 showed antibiosis mechanism. Endophytic fungi has the ability more to colonize at the root (26–60%) than in the stem (20–40%). Fusarium strain MAGR1 has the highest level of endophytic colonization i.e. 60% compared to others. Based on in vivo assay, six endophytic fungi isolates, i.e. Fusarium strain MAGR1, Penicillium strain MAG1, Penicillium strain PAB2, sterile hyphae HAJ1, sterile hyphae HAJ2, and sterile hyphae PBG7, showed the potency to control stem rot disease with inhibition level of 25.5–35.5%

Pengaruh Elektroterapi dan Termoterapi secara in Vitro terhadap Eliminasi Onion yellow dwarf virus Siti Shofiya Nasution; Diny Dinarti; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol. 13 No. 6 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Infection of Onion yellow dwarf virus (OYDV) are reported causing problems in garlic production. Planting virus-free bulbs might help reduce viral disease incidence in the field. This research was aimed to develop method for eliminating OYDV from garlic bulbs using combination of electrotherapy (0, 5, 10, 15, and 20 mA each for 10 minutes) and thermotherapy (23, 28, 33, 38°C each for 4 weeks). Two garlic cultivars, i.e. Sangga Sembalun and Lumbu Hijau were used as seed bulbs for OYDV elimination tests. Virus infection was confirmed using transcription-polymerase chain reaction (RT-PCR). The result showed that thermotherapy at 33 °Cwas the best method to eliminate OYDV in garlic although the efficiency was not the same for all cultivars. The efficiency reached up to 60% for cv. Lumbu Hijau, whereas for cv. Sangga Sembalun only reached up to 40%. Electrotherapy alone or in combination with thermotherapy were not able to produce OYDV-free plantlets.

Co-Authors . SUDARSONO Abdul Muin Adnan ABDUL MUNIF ALI NURMANSYAH Amelia Feryna Bulan Dini Ana Septiana Saputri Anas Dinurrohman Susila Aqlima , Aqlima, nFN Arifin Tasrif Asmar Hasan Asniwita Asniwita Astri Windia Wulandari Wulandari ATI SRI DURIAT Awang Maharijaya Ayu Kartini Parawansa Bambang S. Purwoko Bambang Sapta Purwoko Bonny Poernomo Wahyu Soekarno Bonny Poernomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Bonny Purnomo Wahyu Soekarno Budi Tjahjono Darni Rambu D. Siala Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Diny Dinarti Dono Wahyuno Dono Wahyuno Dwi Astuti Dwi Subekti DWI SUBEKTI Dwi Wiyati Nurul Septariani Dwiwiyati Nurul Septariani Efendi, Darda Efi Toding Tondok Eliza Suryati Rusli Endang Nurhayati Endang Nurhayati Evan P. Ramdan Farida, Naimatul Fitrianingrum Kurniawati, Fitrianingrum GEDE SUASTIKA Gede Suastika Gede Suastika Gede Suastika Gede Suastika GEDE SUASTIKA Giyanto Giyanto Giyanto Hamdayanty Hamdayanty Hanif, Andini Hari Priwiratama Harwan Susetio Heriyanto Syafutra I Wayan Winasa Ifa Manzila Ifa Manzila Ika Mariska Ika Mariska Irsan Nuhantoro ISHAK MANTI Ishak Manti Isti Wulandari Jamsari Jamsari Jati Adiputra John Thomas, John Joni Hidayat, Joni Jumanto Harjosudarmo Jumanto Harjosudarmo JUMANTO HARJOSUDARMO Jumsu Trisno Kadwati Kadwati Kikin H Mutaqin KIKIN HAMZAH MUTAQIN Ladja, Fausiah T. Laksono Trisnantoro Listihani, Listihani Mawarni, Sofi Meity S Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Melinda . Meliyana Memen Surahman Miftakhurohmah Miftakhurohmah Miftakhurohmah Mimi Sutrawati Muh. Taufik Muhamad Syukur Muhammad Herman Muhammad Taufik MUHAMMAD TAUFIK Muhammad Taufik Nada, Azmi Khoirin Neni Gunaeni Niken Nur Kasim, Niken Nur Nissa Fawwaz Adilah NOOR AIDAWATI ORAWAN CHATCHAWANKAN PANICH Prabawati Hyunita Putri Puji Lestari PURNAMA HIDAYAT Purwoko, Bambang Sapto Purwono Purwono Rahayuwati, Sat RAHMI YUNIANTI Ramdan, Evan Purnama RAUF, AUNU Redy Gaswanto Redy Gaswanto, Redy Refa Yulianingsih Reymas M.R. Ruimassa Rina Rachmawati Rita Noveriza RITA NOVERIZA Rita Noveriza Rita Noveriza Rizqiyah, Sakinah Inayatur Rokhana Faizah Roy Ibrahim RR. Ella Evrita Hestiandari Rusmilah Suseno RUSMILAH SUSENO Rustiani, Ummu S. Saiful Akhyar Lubis Sari Nurulita Sari Nurulita Sari, Rahmah Dian Sarsidi S astrosumarjo Sarsidi Sastrosumarjo Satya Nugroho Sayekti, Tri Wahono Dyah Ayu Sherli Anggraini Sientje Mandang Sumaraw Siregar, Ivan Arif Rachman Martua Siti Hafsah Siti Shofiya Nasution Slamet Susanto Sobir Sobir Soemartono Sosromarsono Soemartono Sosromarsono Sri Hartati Sri Hartati Sri Hartati Sri Sulandari Sri Sulandari Sri Sulandari Sriani Sujiprihati Sriani Sujiprihati SRIANI SUJIPRIHATI Sriani Sujiprihati Sugeng Santoso Supramana Suryo Wiyono Susanti Mugi Lestari Tega Kintasari Titiek YULIANTI TRI ASMIRA DAMAYANTI TRI JOKO SANTOSO Trikoesoemaningtyas Trimuri Habazar TRIMURTI HABAZAR Triyani Dumaria Tutik Harmiyati, Tutik Ummu S. Rustiani Ummu Salamah Rustiani Utomo Kartosuwondo UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO Utomo Kartosuwondo UTOMO KARTOSUWONDO Vinsen Willi Wardhana Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Willing Bagariang Zahratul Millah

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