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Formulasi Gel Ekstrak Daun Kelor (Moringa oleifera Lamk) Sebagai Sediaan Antiinflamasi Sugihartini, Nining; Jannah, Syauqul; Yuwono, Tedjo
Pharmaceutical Sciences and Research Vol. 7, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The development of anti-inflammatory gel of Moringa oleifera leaf extract has been performed. This study aimed to determine the physical properties, irritation index and anti-inflammatory activity of gel with various concentration of Moringa oleifera leaf extract. The extract was obtained by maceration method using ethanol 70%. The extracts were formulated in a gel with a concentration of 3%, 6%, 9% by using Carbopol 940 as gelling agent. The gel was evaluated for its physical properties (viscosity, pH, spreadability, adhesivity), irritation index with rabbit test animals and anti-inflammatory activity with mice test animals based on epidermal thickness parameters. The test results showed that the increasing of extract concentration changed the viscosity, pH, adhesivity, epidermal thickness and spreadability of the gel. Based on the results of this study it can be concluded that the optimum concentration of Moringa oleifera leaf extract in gel as anti-inflammatory was 3%.
The Comparison of Analytical Methods for Determination of Eugenol Content in Water Soluble Base from Clove Flower (Syzygium aromaticum) Essential Oil Ointment using UV-Vis Spectrophotometry and High Performance Liquid Chromatography Puput Herawati Said Hasan; Nining Sugihartini; Hari Susanti
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): April-June
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.4522

Abstract

The eugenol content in clove essential oil can be used as a parameter to determine the chemical stability of clove essential oil ointment. Both UV- Vis and HPLC spectrophotometry methods can be used to determine eugenol levels. However, no studies have compared the two methods. This study aims to find out which analytical method is more valid in determining the eugenol content of clove flower essential oil (Syzygium aromaticum) in water-sea based ointments, between the UV-Vis spectrophotometric method and the HPLC method. The parameters in the validation of the analytical method carried out include parameters of linearity, selectivity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). The results of method validation using UV spectrophotometry showed linearity (R = 0.9998), precision (CV = 0.137%), LOD 0.190 µg/mL, LOQ 0.632µg/mL, accuracy (recovery = 100.05%). Method validation using HPLC produced parameters of linearity (R= 0.998), precision (CV = 0.533%), LOD 0.28 µg/mL, LOQ 0.93 µg/mL, accuracy (recovery = 106.43. Average levels of eugenol in water-soluble base MABC ointment using UV spectrophotometry (% concentration = 2.201 ± 0.037) while using HPLC (% concentration = 3.191 ± 0.023). Both methods met the validation requirements. The assay results using both methods met the requirements according to the Indonesian Pharmacopoeia Edition VI. The two methods did not have a significant difference based on the student test, both in the validation parameters and the results of determining the levels of eugenol.
Optimasi Komposisi Madu Kelulut (Trigona Sp) dan Minyak Zaitun (Olive Oil) dalam Salep Erwin Fauzana; Nining Sugihartini; Sapto Yuliani
Jurnal Farmasetis Vol 12 No 2 (2023): Jurnal Farmasetis: Mei 2023
Publisher : LPPM Sekolah Tinggi Ilmu Kesehatan Kendal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.365 KB) | DOI: 10.32583/far.v12i2.914

Abstract

Madu kelulut (Trigona sp) dan minyak zaitun (Olive oil) telah lama diketahui memiliki potensi farmakologi dalam penyembuhan luka diabetes. Penelitian ini bertujuan untuk mengetahui komposisi optimum kedua bahan tersebut dalam sediaan salep. Tahap pertama orientasi kadar madu kelulut dan minyak zaitun (1:1) dalam salep dengan kadar 5%; 10%; 15%; 20%; 25% dan 30% berdasarkan parameter pH dan viskositas. Tahap kedua optimasi komposisi madu kelulut dan minyak zaitun dengan menggunakan aplikasi Design Expert 13® metode Simplex lattice design. Formula dievaluasi berdasarkan parameter pH, viskositas dan daya hambat bakteri menggunakan S.aureus ATCC 25923. Uji tahap pertama menunjukkan bahwa peningkatan konsentrasi bahan aktif menyebabkan penurunan pH dan viskositas. Hasil uji tahap kedua menunjukkan persamaan untuk parameter pH adalah Y=5,58965(A)+9,25632(B) 4,88017AB, viskositas adalah Y=11502,2552(A)+481,8706(B)+1,318x105AB dan daya hambat bakteri adalah Y=-4,148A+24,409B+15,947AB+72,654AB(A-B). Berdasarkan hasil penelitian diketahui konsentrasi optimum bahan aktif adalah 20% dengan komposisi optimum dari madu kelulut dan minyak zaitun pada perbandingan 0,586 dan 0,414.  
EVALUASI SIFAT FISIK SEDIAAN LOTION DENGAN VARIASI KONSENTRASI EKSTRAK DAUN KELOR (Moringa oleifera L.) Atri Sri Ulandari; Nining Sugihartini
Jurnal Penelitian dan Kajian Ilmiah Kesehatan Politeknik Medica Farma Husada Mataram Vol. 6 No. 1 (2020): JURNAL PENELITIAN DAN KAJIAN ILMIAH KESEHATAN POLITEKNIK MEDICA FARMA HUSADA MA
Publisher : Politeknik Medica Farma Husada Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (582.836 KB) | DOI: 10.33651/jpkik.v6i1.57

Abstract

Daun kelor memiliki banyak manfaat diantaranya adalah sebagai antioksidan karena terdapat kandungan senyawa flavonoid. Flavonoid telah terbukti secara ilmiah berkhasiat sebagai penangkal radikal bebas (antioksidan) dari paparan sinar matahari. Ekstrak etanol 50% daun kelor mengandung flavonoid yang berkhasiat sebagai tabir surya sehingga diformulasikan dalam bentuk lotion. Penelitian ini bertujuan untuk mengetahui pengaruh variasi konsentrasi ekstrak daun kelor terhadap sifat fisik sediaan lotion. Penelitian ini diawali dengan melakukan formulasi sediaan lotion dengan penambahan konsentrasi ekstrak daun kelor 1% (F1); 3% (F2); 5% (F3). Lotion dievaluasi sifat fisiknya dengan parameter pH, daya sebar, daya lekat, viskositas, dan stabilitas fisiknya dan diuji sebagai tabir surya. Data yang diperoleh dianalisis dengan statistik One Way Anova dengan taraf kepercayaan 95% untuk mengetahui adanya perbedaan yang signifikan antar kelompok perlakuan. Hasil penelitian menunjukkan bahwa variasi konsentrasi ekstrak tidak mempengaruhi pH dan daya lekat. Peningkatan konsentrasi menyebabkan penurunan daya sebar (P<0,05) dan peningkatan viskositas (p<0,05). Lotion dengan konsentrasi ekstrak daun kelor 1% tidak mengalami pemisahan setelah sentrifugasi.
Determination of Total Phenolic Levels in Ethanol Extract of Moringa (Moringa oleifera L.) Leaves based on Differences in Growing Sites Any Guntarti; Nining Sugihartini; Siti Athiyah Umaiyah; Nina Salamah
Journal of Food and Pharmaceutical Sciences Vol 9, No 1 (2021): J.Food.Pharm.Sci
Publisher : Institute for Halal Industry and System (IHIS) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.1337

Abstract

Moringa oleifera L. have good nutritional content including phenolic compounds which can be used as antioxidants and can grow in lowlands and highlands. The purpose of this study was to determine the total phenolic content of the ethanol extract of M. oleifera leaves with variations in the area of ​​collection. The 50% ethanol extract was obtained from the simplicia of M. oleifera leaves by using the maceration method. Analysis of total phenolic content in the extract was carried out using a spectrophotometer instrument with the addition of reagent Folin-Ciocalteu and gallic acid as standard. The results of total phenolic content in Sleman, Wonosari, and Wonosobo areas were (127.87 ± 2.71) mg GAE / g extract, (99.40 ± 2.68) mg GAE / g extract, and (142 , 92 ± 1.81) mg GAE / g extract. The highest phenolic content in the ethanol extract of moringa leaves was found in Wonosobo areas.
Effect of ethanol solvent concentrations in pepino melon fruit (Solanum muricatum Aiton) extraction on total flavonoid, phenolic, and beta-carotene content Girly Risma Firsty; Nining Sugihartini; Sri Mulyaningsih
Pharmaciana Vol 13, No 2 (2023): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v13i2.25226

Abstract

The quality of an extract is determined by the type and level of compounds contained therein. The solvent is one of the elements that influences the extract's quality. Therefore, extraction must be carried out using a solvent that can extract bioactive substances. This study was aimed at obtaining the optimal concentration of ethanol in extracting flavonoids, phenolics, and beta-carotene from pepino melon fruit. In this study, dried pepino melon fruit was extracted using ethanol of 50%, 70%, and 96%. The extracts were analyzed qualitatively for phenolic and flavonoid using thin layer chromatography (TLC), and quantitatively using a spectrophotometry. Meanwhile, beta-carotene levels were determined using high pressure liquid chromatography (HPLC). The data of level flavonoid, phenolic, and beta-carotene were statistically analyzed using multivariate analysis of variance. The results showed that 50%, 70%, and 96% ethanol solvent produced extracts with a yield value of 51.8%; 87.4%; 54.6%; total flavonoid content of 0.298 ± 0.04 mgQE/g; 0.559 ± 0.03 mgQE/g; 0.289 ± 0.01 mgQE/g; total phenolic content of 4.763 ± 0.08 mgGAE/g; 3.631 ± 0.12 mgGAE/g; 3.317 ± 0.10 mgGAE/g; beta-carotene level of 0.157 ± 0.02 mg/g; 0.910 ± 0.16 mg/g; 1.054 ± 0.13 mg/g, respectively. The statistical analysis showed that there was a significant difference in different ethanol concentrations in extraction with the content of total flavonoids, total phenolics, and beta-carotene (p<0.05). The optimal ethanol solvent for extracting flavonoids, phenolic compounds, and beta-carotene from pepino melon fruit was 70% ethanol.
Pengaruh Purifikasi Terhadap Kandungan Zat Aktif Dan Aktivitas Antioksidan Ekstrak Etanol 50% Daun Kelor (Moringa oleifera L.) Diyan Sakti Purwanto; Hari Susanti; Nining Sugihartini
CERATA Jurnal Ilmu Farmasi Vol 12 No 2 (2021): Cerata Jurnal Ilmu Farmasi
Publisher : Universitas Muhammadiyah Klaten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61902/cerata.v12i2.196

Abstract

Purifikasi ekstrak etanol 50% daun kelor (Moringa oleifera L.) dilakukan untuk meningkatkan kandungan zat aktif flavanoid, β-karoten, saponin dan tanin yang memiliki aktivitas sebagai antioksidan. Tujuan penelitian ini adalah mengetahui jenis pelarut yang paling optimal antara etil asetat dan n-heksan dalam fraksinasi ekstrak etanol 50% daun kelor. Penelitian dimulai ekstraksi etanol 50% daun kelor dengan maserasi dan dilanjutkan evaporator kemudian waterbath sampai diperoleh ekstrak kental. Ekstrak kental yang diperoleh dari ekstraksi selanjutnya dilakukan purifikasi. Fraksinasi dengan melarutkan ekstrak kental etanol 50% daun kelor (E1) dan aquades pada suhu 70ºC kemudian ditambahkan etil asetat pada corong pisah hingga di peroleh fraksi etil asetat (E2), ekstrak kental etanol 50% daun kelor (E1) ditambahkan n-heksan pada corong pisah hingga terjadi pemisahan fase sehingga diperoleh fraksi n-heksana (E3). Ekstrak dan fraksi dievaluasi parameter meliputi kadar air ekstrak dengan gravimetri, kadar β-karoten dengan HPLC dan aktivitas antioksidan dengan DPPH. Data dianalisis statistik dengan taraf kepercayaam 95%. Hasil Penelitian menunjukkan bahwa kadar air ekstrak etanol 50% daun kelor adalah 4,16 dengan CV 4,62%. Kadar β-karoten paling optimal pada fraksi n-heksana (E3) kemudian etil asetat (E2) dan etanol 50% (E1) yaitu 1.48 ± 0.01%, 1.19 ± 005% dan 0.73 ± 0.01%. Sedangkan aktivitas antioksidan (IC50) paling optimal pada fraksi n-heksana (E3) kemudian etanol 50% (E1) dan etil asetat (E2) yaitu 40.83 ± 0.04 ug/ml, 47.75 ± 0.09 ug/ml dan 58.79 ± 0.10ug/ml. Nilai aktivitas antioksian menunjukkan pada fraksi n-heksana dan ekstrak etanol 50% termasuk dalam kategori sangat kuat sedangkan fraksi etil asetat termasuk dalam kategori kuat sehingga berpotensi sebagai antioksidan.
A Study progress on moringa seed oil with microemulsion: bibliometrics analysis of year 2012-2023 chasanah, Uswatun; Lalu Muhammad Irham; Nining Sugihartini; Sapto Yuliani
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol. 8 No. 1 (2023)
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/farmasains.v8i1.28978

Abstract

Transdermal drug delivery systems increase the effectiveness of a drug substance, and one of them is a microemulsion. Moringa oil has anti-inflammatory, antioxidant, and antibacterial activity. The purpose of this study is to conduct a bibliometric analysis research on moringa oil formulated as microemulsions. A total of 5 English articles from the Scopus databases from 2012 to 2023 US sources were used in the bibliometrics analysis of Moringa oleifera seeds oil related to microemulsions. The bibliometrics analysis shows research trends, including the most used keywords depicted in visualizations using the VOSViewer application, article publishers, affiliations, and the author's country of origin. The most used keywords are microemulsions, microemulsion, Moringa oleifera, antioxidant activities, and antioxidants. The most publishers are Key Engineering Materials, the most affiliated are Chiang Mai University and Thamasat University, and most authors are from Thailand. The study of microemulsion formulations of Moringa seed oil has yet to be widely carried out but has been cited by other researchers.
FORMULASI SEDIAAN FACE WASH EKSTRAK DAUN KELOR (Moringa oleifera L.) DAN EKSTRAK BIJI KOPI ROBUSTA (Coffea canephora P.) DALAM SEDIAAN GEL DAN CAIR Rahmat Hidayat; Nining Sugihartini; Hari Susanti
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 9 No 1 (2024)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v9i1.762

Abstract

Sediaan pembersih wajah (face wash) berfungsi untuk membersihkan sekaligus mencegah timbulnya masalah kulit wajah. Penambahan bahan aktif berupa ekstrak daun kelor dan ekstrak biji kopi diharapkan akan meningkatkan manfaat sediaan face wash tersebut. Tujuan dari penelitian ini untuk mengetahui jenis sediaan yang paling optimal antara sediaan gel dan cair serta variasi komposisi yang optimal antara kedua ekstrak dalam formulasi face wash. Face wash diformulasikan dalam bentuk sediaan gel dan cair dengan variasi komposisi ekstrak daun kelor (Moringa oleifera L.) dan ekstrak biji  kopi robusta (Coffea canephora P.) secara berturut-turut yaitu 1:1, 1:2, 2:1. Sediaan dievaluasi berdasarkan parameter organoleptis, pH, viskositas, tinggi busa dan stabilitas busa serta daya hambat terhadap bakteri Staphylococcus aureus. Data yang diperoleh kemudian di analisa Two Way Anova dalam aplikasi SPSS versi 23. Berdasarkan uji diketahui bahwa face wash gel lebih baik pada uji organoleptis, pH, tinggi busa dan stabilitas busa, viskositas dan daya hambat terhadap bakteri. Peningkatan komposisi ekstrak biji kopi robusta menyebabkan peningkatan pH pada jenis sediaan face wash gel maupun cair. Berdasarkan data maka dapat disimpulkan jenis sediaan face wash gel pada varian komposisi zat aktif ekstrak biji kopi robusta 10% dan ekstrak daun kelor 20% lebih baik dan paling sesuai standar yang ditetapkan SNI (Standar Nasional Indonesia). Kata kunci : Face wash, Ekstrak daun kelor, Ekstrak kopi robusta, gel
Antioxidant, Tyrosinase Inhibition Activity, and In Vitro SPF Evaluation of Pepino Fruit Extract (Solanum muricatum Aiton) in Different Solvent Types and Concentrations Sugihartini, Nining; Firsty, Girly Risma; Laila, Wahyu Kumil; Mulyaningsih, Sri; Rais, Ichwan Ridwan
Pharmaceutical Sciences and Research Vol. 11, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Solvent is a key factor that affects the effectiveness of active compound extraction from plant materials (simplisia). This study aimed to determine the optimal type and concentration of solvent used in the extraction of pepino fruit based on the parameter of antioxidant and tyrosinase inhibition activity, as well as Sun Protection Factor (SPF) value. The extraction was carried out using the maceration method with ethanol or ethyl acetate as the solvent, each at concentrations of 50%, 70%, and 96%, respectively. The antioxidant activity of the extracts was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The inhibition of tyrosinase and the determination of the SPF value were carried out using in vitro test. The results showed that the ethyl acetate extract was better than that of the ethanol extract in terms of antioxidant activity, tyrosinase inhibition, and SPF value. In the ethyl acetate solvent, a concentration of 96% provided the strongest antioxidant, tyrosinase inhibition activity, and the second highest in SPF test. It can be concluded that the optimal solvent for extracting pepino fruit as promising compound for sunscreen formulation is 96% ethyl acetate.
Co-Authors . Sismindari Achmad Fudholi Achmad Fudholi Achmad Fudholi Achmad Fudholi Aditya Noviadi Rakhmatullah Afriyani, Neti Agung Putu S.P.Kristyawan Aina Fatkhil Haque, Aina Fatkhil Aji Pamungkas Alvionida, Fitra Amraini Amelia Ani Guntarti Any Guntarti Any Guntarti Astryna, Syarifah Yanti Atri Sri Ulandari Auliah Rahmi Latif Azis Ikhsanudin Bayu Herdi Al Huda Deasy Vanda Pertiwi Delia Komala Sari, Delia Komala Desrianti, Cindy Desyandri Desyandri Diah Pratimasari Diyan Sakti Purwanto Diyan Sakti Purwanto Endah Nurlaela Endah Nurlaela Endang Lukitaningsih Erma Pranawati ERMAWATI, DIAN EKA Erna Astuti Erna Astuti Erwin Fauzana Evi Nuryanti Fadzil Latifah Firsty, Girly Risma Fitra Alvionida Fitri Rahmawanti Fitriah Ardiawijianti Iriani Fudholi, Achmad Fudholi, Achmad Gina Lestari Gina Lestari, Gina Girly Risma Firsty Gita Puspita Hafid, Indah Fitria Hana Hanifah Hari Susanti Hari Susanti Heru Sasongko Ichwan Ridwan Rais Iis Wahyuningsih Jannah, Syauqul Kintoko Kintoko Kintoko, Kintoko Kurniawan, Muhammad Fariez Kusuma, Nyoman Rudi Laela Hayu Nurani Laila, Wahyu Kumil Lalu Muhammad Irham Lela Sulastri, Lela Lena Maulina Lena Maulina, Lena M Alif Fajri Marchaban . Miratun Syarifah Mohamad Ikram Muhammad Alfian, Muhammad Muhammad Fariez Kurniawan Muhammad Fariez Kurniawan Muhammad Yusron Maulana El-Yunusi Mukhlishah, Neneng Rachmalia Izzatul Nanik Sulistyani Nina Salamah NINGRUM, TYAS SARI RATNA Novitasari, Putri Rachma Nugrahini, Febrina Nurhayati Nurhayati Nuri Ari Efiana Nurlaela, Endah Olvia Becatami Widyantoro Olvia Becatami Widyantoro, Olvia Becatami Pertiwi, Deasy Vanda Pramono, Suwidjiyo Pramono, Suwidjiyo Puput Herawati Said Hasan Rahmat Hidayat Rahmawati, Desty Restia Raka Sukmabayu W.S Ratih Saridewi Rikadyanti Rikadyanti Rinanda Yeshi Wiradhika Ririn Suharsanti, Ririn Ristiono, Hendi Rosmiyanti, Yanti Rukaya, Benazir Evita Ryan Radix Rahadhian, Muhammad Sapto Yuliani Sapto Yuliani Sapto Yuliani Sismindari . Sismindari Sismindari Sismindari Sismindari Sismindari Sismindari Sismindari, Sismindari Sismindari, Sismindari Siti Athiyah Umaiyah Siti Ayu Marlina Sri Mulyaningsih Sri Mulyaningsih Srimuliani Arbie Suwidjiyo Pramono Suwidjiyo Pramono Suwidjiyo Pramono Suwidjiyo Pramono Suwidjiyo Pramono. Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Tedjo Yuwono Teuku Nanda Saifullah, Teuku Nanda Trecya Fujiastuti Trecya Fujiastuti, Trecya Tusilowati, Defita Andri Ulfa Ramdhani M Uswatun Chasanah Vivi Sophia Waliyyin Razan Qanit wardhani, Riska risty Widyantoro, Olvia Becatami Widyasari Putranti Wisnu Kundarto Wuryandari, Tutik Wuryandari, Tutik Yayan Rizikiyan Zaenab Zaenab Zainab Zainab Zainab Zainab Zainab